Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,512,523 A→G A223A (GCA→GCG)  b1442 → putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,512,5230AG100.0% 634.4 / NA 185A223A (GCA→GCG) b1442putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (88/97);  total (88/97)

Only 100 of 198 total aligned reads displayed.

TATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACT  >  NC_000913/1512433‑1512612
                                                                                          |                                                                                         
tatCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGcgcgcg                                                                                         >  1:2730859/1‑93 (MQ=255)
 atCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGt                                                                                        <  2:2794504/93‑1 (MQ=255)
 atCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGt                                                                                        <  2:6105889/93‑1 (MQ=255)
   cTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTcc                                                                                      <  1:1644641/93‑1 (MQ=255)
    tGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCa                                                                                     <  2:1530228/93‑1 (MQ=255)
    tGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCa                                                                                     >  2:4966017/1‑93 (MQ=255)
        tGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGAc                                                                                 >  1:1005377/1‑93 (MQ=255)
        tGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGAc                                                                                 >  1:2087881/1‑93 (MQ=255)
         gCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACa                                                                                >  2:200167/1‑93 (MQ=255)
         gCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACa                                                                                >  2:3094271/1‑93 (MQ=255)
            gTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAAc                                                                             >  2:1951723/1‑93 (MQ=255)
            gTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAAc                                                                             <  2:1191331/93‑1 (MQ=255)
             ttCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAAcc                                                                            >  2:2268890/1‑93 (MQ=255)
             ttCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAAcc                                                                            >  2:2732481/1‑93 (MQ=255)
              tCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCt                                                                           <  2:161356/93‑1 (MQ=255)
                aTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCttt                                                                         >  1:1784305/1‑93 (MQ=255)
                aTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCttt                                                                         >  2:37856/1‑93 (MQ=255)
                aTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCttt                                                                         <  1:3050449/93‑1 (MQ=255)
                 tGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTc                                                                        <  2:2500270/93‑1 (MQ=255)
                 tGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTc                                                                        >  2:6056658/1‑93 (MQ=255)
                  gATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCg                                                                       >  1:6042965/1‑93 (MQ=255)
                   aTCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGc                                                                      <  1:5609625/93‑1 (MQ=255)
                   aTCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGc                                                                      >  1:767472/1‑93 (MQ=255)
                   aTCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGc                                                                      >  1:5826859/1‑93 (MQ=255)
                    tCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCt                                                                     <  1:4569106/93‑1 (MQ=255)
                      cTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTAt                                                                   <  2:2395528/93‑1 (MQ=255)
                       tGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTAtg                                                                  >  1:3842002/1‑93 (MQ=255)
                         cccGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTAtgtg                                                                <  1:2933682/93‑1 (MQ=255)
                         cccGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTAtgtg                                                                <  2:4099516/93‑1 (MQ=255)
                            gTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATgtggtg                                                             >  2:6271130/1‑93 (MQ=255)
                            gTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATgtggtg                                                             <  1:799867/93‑1 (MQ=255)
                            gTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATgtggtg                                                             >  1:5760280/1‑93 (MQ=255)
                            gTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATgtggtg                                                             >  1:5051999/1‑93 (MQ=255)
                              tCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGtgct                                                           <  1:4076885/93‑1 (MQ=255)
                                aGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                                         <  1:137797/93‑1 (MQ=255)
                                aGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                                         >  1:633515/1‑93 (MQ=255)
                                 ggcggcGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGcc                                                        >  2:5523996/1‑93 (MQ=255)
                                  gcggcgCGTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                                       <  1:3230567/93‑1 (MQ=255)
                                    ggcgCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCt                                                     >  1:3424056/1‑93 (MQ=255)
                                     gcgcTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTg                                                    <  2:1756469/93‑1 (MQ=255)
                                      cgcTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTgg                                                   <  1:5223584/93‑1 (MQ=255)
                                        cTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCa                                                 >  1:5923116/1‑93 (MQ=255)
                                        cTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCa                                                 >  1:3981188/1‑93 (MQ=255)
                                         ttGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCaa                                                <  1:5289800/93‑1 (MQ=255)
                                         ttGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCaa                                                >  2:4008945/1‑93 (MQ=255)
                                           gAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATc                                              >  2:372082/1‑93 (MQ=255)
                                            aGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                                             >  2:2163342/1‑93 (MQ=255)
                                            aGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                                             <  1:1219017/93‑1 (MQ=255)
                                            aGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                                             >  2:3694917/1‑93 (MQ=255)
                                             gCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATccc                                            >  2:3535616/1‑93 (MQ=255)
                                              cttttGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCg                                           <  2:458444/91‑1 (MQ=255)
                                                 ttGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGt                                        <  1:4303726/93‑1 (MQ=255)
                                                 ttGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGt                                        <  2:5173340/93‑1 (MQ=255)
                                                 ttGCCGCCGTCATTGTTGCAGGCATCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGt                                        <  1:4109905/93‑1 (MQ=255)
                                                    ccgccgTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTAtt                                     <  2:734624/93‑1 (MQ=255)
                                                    ccgccgTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTAtt                                     <  1:5004576/93‑1 (MQ=255)
                                                     cgccgTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTg                                    <  2:297035/93‑1 (MQ=255)
                                                       ccgTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGcc                                  >  1:2550178/1‑93 (MQ=255)
                                                        cgTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg                                 <  1:4219262/93‑1 (MQ=255)
                                                         gTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGc                                >  1:1610020/1‑93 (MQ=255)
                                                          tCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCt                               <  2:5068782/93‑1 (MQ=255)
                                                           cATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTg                              <  2:635443/93‑1 (MQ=255)
                                                           cATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTg                              >  2:3148214/1‑93 (MQ=255)
                                                           cATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGCGGTGCTGCCGCTGGCAATCCCGGGAATTGCCGccg                              >  1:1997150/1‑91 (MQ=255)
                                                            aTTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTgg                             <  1:977569/93‑1 (MQ=255)
                                                             ttgttgCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGc                            <  2:2461433/93‑1 (MQ=255)
                                                             ttcttgCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGc                            <  1:555014/90‑1 (MQ=255)
                                                               gttgCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGctc                          <  1:6205547/93‑1 (MQ=255)
                                                                ttgCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGctct                         <  1:6082487/93‑1 (MQ=255)
                                                                   cAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATc                      <  1:1724558/93‑1 (MQ=255)
                                                                    agccGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt                     <  1:2236510/90‑1 (MQ=255)
                                                                    aGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt                     <  1:6170633/93‑1 (MQ=255)
                                                                    aGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt                     <  2:3273861/93‑1 (MQ=255)
                                                                    aGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt                     <  2:3408597/93‑1 (MQ=255)
                                                                     ggCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCtt                    <  2:5380585/93‑1 (MQ=255)
                                                                       cGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTa                  <  1:6291805/93‑1 (MQ=255)
                                                                        gTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAc                 >  1:228622/1‑93 (MQ=255)
                                                                         tCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAcc                >  1:5868113/1‑93 (MQ=255)
                                                                          cGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCt               >  1:4645160/1‑93 (MQ=255)
                                                                          cGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCt               >  2:5643294/1‑93 (MQ=255)
                                                                          cGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCt               <  1:4253020/93‑1 (MQ=255)
                                                                           ggCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCtt              <  2:4804746/93‑1 (MQ=255)
                                                                            gCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTtc             <  2:2049645/93‑1 (MQ=255)
                                                                            gCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTtc             <  2:6170305/93‑1 (MQ=255)
                                                                             cTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTtct            <  1:381414/93‑1 (MQ=255)
                                                                              tGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTtctc           >  2:5779215/1‑93 (MQ=255)
                                                                              tGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTtctc           <  1:4724058/93‑1 (MQ=255)
                                                                              tGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTtctc           >  1:3735368/1‑93 (MQ=255)
                                                                               gATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctca          <  2:1050459/93‑1 (MQ=255)
                                                                               gATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctca          >  1:2956172/1‑93 (MQ=255)
                                                                                aTCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcac         <  2:4237408/93‑1 (MQ=255)
                                                                                aTCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcac         <  1:3478746/93‑1 (MQ=255)
                                                                                  ctcGGCGCGCGTCCACGACAATCCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcactc       <  1:1446066/93‑1 (MQ=255)
                                                                                  ctcGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcactc       <  2:1859568/93‑1 (MQ=255)
                                                                                   tcGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcactca      >  2:4755253/1‑93 (MQ=255)
                                                                                   tcGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcactca      >  1:3434819/1‑93 (MQ=255)
                                                                                   ccGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTctcactca      <  1:6405157/92‑1 (MQ=255)
                                                                                      gcgcgcGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCacac   <  2:4398052/93‑1 (MQ=255)
                                                                                      gcgcgcGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCacac   >  1:1896718/1‑93 (MQ=255)
                                                                                       cgcgcgTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACt  <  1:3677327/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACT  >  NC_000913/1512433‑1512612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGG  >  NC_000913/1512430‑1512615
                                                                                             |                                                                                            
CTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCG                                                                                              <  SRR4302534.1984762/94‑1 (MQ=60)
CTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCG                                                                                              <  SRR4302534.179282/94‑1 (MQ=60)
 TATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGC                                                                                             >  SRR4302534.6044905/1‑94 (MQ=60)
  ATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCG                                                                                            >  SRR4302534.2802865/1‑94 (MQ=60)
   TATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGT                                                                                           >  SRR4302534.5656441/1‑94 (MQ=60)
    ATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTC                                                                                          >  SRR4302534.1860942/1‑94 (MQ=60)
      CTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCA                                                                                        >  SRR4302534.3013129/1‑94 (MQ=60)
      CTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCA                                                                                        <  SRR4302534.1687512/94‑1 (MQ=60)
        GGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACG                                                                                      <  SRR4302534.3870781/94‑1 (MQ=60)
        GGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACG                                                                                      <  SRR4302534.1666705/94‑1 (MQ=60)
          TTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGAC                                                                                    >  SRR4302534.1031167/1‑94 (MQ=60)
          TTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGAC                                                                                    >  SRR4302534.2142600/1‑94 (MQ=60)
             CCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAA                                                                                 >  SRR4302534.4384229/1‑94 (MQ=60)
                  CATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTT                                                                            >  SRR4302534.1830640/1‑94 (MQ=60)
                   ATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTC                                                                           <  SRR4302534.3130473/94‑1 (MQ=60)
                    TGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCT                                                                          <  SRR4302534.5377472/94‑1 (MQ=60)
                    TGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCG                                                                          >  SRR4302534.6207241/1‑94 (MQ=60)
                    TGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCG                                                                          >  SRR4302534.4786994/1‑94 (MQ=60)
                     GATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCaa                                                                         >  SRR4302534.1700567/1‑92 (MQ=60)
                     GATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGC                                                                         >  SRR4302534.5985021/1‑94 (MQ=60)
                     GATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGC                                                                         >  SRR4302534.786546/1‑94 (MQ=60)
                      ATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCT                                                                        <  SRR4302534.5761377/94‑1 (MQ=60)
                       TCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTA                                                                       <  SRR4302534.4692974/94‑1 (MQ=60)
                        CCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTAT                                                                      >  SRR4302534.2460424/1‑94 (MQ=60)
                         CTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATG                                                                     >  SRR4302534.3945203/1‑94 (MQ=60)
                          TGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGT                                                                    >  SRR4302534.6030942/1‑94 (MQ=60)
                           GCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTG                                                                   >  SRR4302534.2937332/1‑94 (MQ=60)
                            CCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGG                                                                  <  SRR4302534.3010691/94‑1 (MQ=60)
                              CGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTG                                                                >  SRR4302534.5188431/1‑94 (MQ=60)
                              CGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTG                                                                >  SRR4302534.5916544/1‑94 (MQ=60)
                               GTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGC                                                               <  SRR4302534.819955/94‑1 (MQ=60)
                                 TCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTG                                                             <  SRR4302534.5744788/94‑1 (MQ=60)
                                 TCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTG                                                             <  SRR4302534.4186293/94‑1 (MQ=60)
                                 TCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTG                                                             <  SRR4302534.2785347/94‑1 (MQ=60)
                                  CAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGC                                                            >  SRR4302534.648888/1‑94 (MQ=60)
                                   AGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCC                                                           <  SRR4302534.141399/94‑1 (MQ=60)
                                   AGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCC                                                           <  SRR4302534.4049515/94‑1 (MQ=60)
                                    GGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCG                                                          <  SRR4302534.38079/94‑1 (MQ=60)
                                    GGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCG                                                          <  SRR4302534.991226/94‑1 (MQ=60)
                                     GCGGCGCGTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGC                                                         <  SRR4302534.3316423/94‑1 (MQ=60)
                                      CGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCT                                                        >  SRR4302534.3516056/1‑94 (MQ=60)
                                      CGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCT                                                        <  SRR4302534.3643881/94‑1 (MQ=60)
                                       GGCGCTAGAGCGTTTGCCGCTGTCATTGTTGCAGGCGTCGGTTGATCTCGGCGCGCGTCCACGACAAGCCTTTCGCTATGTGGTGCTGCCGCTG                                                       <  SRR4302534.786894/94‑1 (MQ=60)
                                        GCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGG                                                      <  SRR4302534.6132988/94‑1 (MQ=60)
                                        GCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTCCGCTATGTGGTGCTGCCGCTGG                                                      <  SRR4302534.6003180/94‑1 (MQ=60)
                                         CGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGC                                                     <  SRR4302534.5364584/94‑1 (MQ=60)
                                         CGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGC                                                     >  SRR4302534.767635/1‑94 (MQ=60)
                                          GCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCA                                                    >  SRR4302534.6083944/1‑94 (MQ=60)
                                          GCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCA                                                    >  SRR4302534.4087716/1‑94 (MQ=60)
                                          GCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCA                                                    <  SRR4302534.2007139/94‑1 (MQ=60)
                                           CTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAA                                                   >  SRR4302534.134664/1‑94 (MQ=60)
                                           CTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAA                                                   >  SRR4302534.3934422/1‑94 (MQ=60)
                                           CTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAA                                                   >  SRR4302534.633875/1‑94 (MQ=60)
                                            TTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAAT                                                  <  SRR4302534.5432586/94‑1 (MQ=60)
                                            TTGAGCGTTGGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAAT                                                  >  SRR4302534.6473361/1‑94 (MQ=60)
                                               AGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCC                                               <  SRR4302534.1251109/94‑1 (MQ=60)
                                               AGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCC                                               <  SRR4302534.80305/94‑1 (MQ=60)
                                               AGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCC                                               <  SRR4302534.5165531/94‑1 (MQ=60)
                                                  GTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGG                                            <  SRR4302534.3058086/94‑1 (MQ=60)
                                                   TTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGT                                           >  SRR4302534.4909255/1‑94 (MQ=60)
                                                    TTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTA                                          <  SRR4302534.1559360/94‑1 (MQ=60)
                                                    TTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTA                                          <  SRR4302534.4420393/94‑1 (MQ=60)
                                                    TTGCCGCCGTCATTGTTGCAGGCATCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTA                                          <  SRR4302534.4220458/94‑1 (MQ=60)
                                                      GCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATT                                        <  SRR4302534.3253134/94‑1 (MQ=60)
                                                       CCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTG                                       <  SRR4302534.5139865/94‑1 (MQ=60)
                                                         GCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCC                                     >  SRR4302534.5227321/1‑94 (MQ=60)
                                                         GCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCC                                     >  SRR4302534.2617790/1‑94 (MQ=60)
                                                           CGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGC                                   >  SRR4302534.1652054/1‑94 (MQ=60)
                                                           CGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGC                                   >  SRR4302534.638546/1‑94 (MQ=60)
                                                           CGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGC                                   <  SRR4302534.4333017/94‑1 (MQ=60)
                                                           CGTCATTGTTGCAGGCGTCGGCTGATCTCGGCCCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTAgtggcct                                   >  SRR4302534.1043851/1‑87 (MQ=60)
                                                             TCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTG                                 <  SRR4302534.4098196/94‑1 (MQ=60)
                                                             TCATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGCGGTGCTGCCGCTGGCAATCCCGGGAATTGCCGCCG                                 >  SRR4302534.2049453/1‑94 (MQ=60)
                                                             TCATGGTTGCAGACGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAACCCCGGGTATTGCCGCTG                                 <  SRR4302534.6306683/94‑1 (MQ=60)
                                                              CATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGG                                >  SRR4302534.1786718/1‑94 (MQ=60)
                                                               ATTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGC                               <  SRR4302534.1002520/94‑1 (MQ=60)
                                                                TTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCT                              <  SRR4302534.1433990/94‑1 (MQ=60)
                                                                TTGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCT                              <  SRR4302534.4351498/94‑1 (MQ=60)
                                                                TTCTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCT                              <  SRR4302534.568696/94‑1 (MQ=60)
                                                                 TGTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTC                             >  SRR4302534.5930180/1‑94 (MQ=60)
                                                                  GTTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCT                            <  SRR4302534.6374277/94‑1 (MQ=60)
                                                                   TTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTA                           <  SRR4302534.5764165/94‑1 (MQ=60)
                                                                   TTGCAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTA                           <  SRR4302534.6247899/94‑1 (MQ=60)
                                                                      CAGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCT                        <  SRR4302534.1769346/94‑1 (MQ=60)
                                                                       AGGCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTT                       <  SRR4302534.6338485/94‑1 (MQ=60)
                                                                       AGCCGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTT                       <  SRR4302534.2295775/94‑1 (MQ=60)
                                                                          CGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAC                    <  SRR4302534.6462021/94‑1 (MQ=60)
                                                                          CGTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAC                    >  SRR4302534.234594/1‑94 (MQ=60)
                                                                           GTCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACC                   >  SRR4302534.6027444/1‑94 (MQ=60)
                                                                            TCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCT                  <  SRR4302534.4204803/94‑1 (MQ=60)
                                                                            TCGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCT                  >  SRR4302534.4771252/1‑94 (MQ=60)
                                                                             CGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTT                 >  SRR4302534.3425441/1‑94 (MQ=60)
                                                                             CGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTT                 <  SRR4302534.4367777/94‑1 (MQ=60)
                                                                             CGGCTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTT                 >  SRR4302534.4473732/1‑94 (MQ=60)
                                                                                CTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTC              <  SRR4302534.391165/94‑1 (MQ=60)
                                                                                CTGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTC              >  SRR4302534.3835714/1‑94 (MQ=60)
                                                                                 TGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCA             >  SRR4302534.3033651/1‑94 (MQ=60)
                                                                                 TGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCA             <  SRR4302534.4852354/94‑1 (MQ=60)
                                                                                 TGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCA             >  SRR4302534.2332331/1‑94 (MQ=60)
                                                                                 TGATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCA             >  SRR4302534.355303/1‑94 (MQ=60)
                                                                                   ATCTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACT           <  SRR4302534.3572250/94‑1 (MQ=60)
                                                                                     CTCGGCGCGCGTCCACGACAATCCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCA         <  SRR4302534.1484126/94‑1 (MQ=60)
                                                                                     CTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCA         >  SRR4302534.6211544/1‑94 (MQ=60)
                                                                                     CTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCA         >  SRR4302534.3527118/1‑94 (MQ=60)
                                                                                     CTCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCA         <  SRR4302534.4330434/94‑1 (MQ=60)
                                                                                      TCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCAC        >  SRR4302534.3517670/1‑94 (MQ=60)
                                                                                      CCGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCAC        <  SRR4302534.6578064/94‑1 (MQ=60)
                                                                                       CGGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACA       >  SRR4302534.4693757/1‑94 (MQ=60)
                                                                                        GGCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACAC      >  SRR4302534.1946183/1‑94 (MQ=60)
                                                                                         GCGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACT     >  SRR4302534.2139629/1‑94 (MQ=60)
                                                                                          CGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTG    >  SRR4302534.589453/1‑94 (MQ=60)
                                                                                          CGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTG    >  SRR4302534.3557056/1‑94 (MQ=60)
                                                                                          CGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTG    <  SRR4302534.3776196/94‑1 (MQ=60)
                                                                                          CGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTG    >  SRR4302534.2772587/1‑94 (MQ=60)
                                                                                          CGCGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTG    <  SRR4302534.2184189/94‑1 (MQ=60)
                                                                                           GCGCGTCCACGACAACCCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGG   <  SRR4302534.2471343/94‑1 (MQ=60)
                                                                                            CGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGG  <  SRR4302534.2820884/94‑1 (MQ=60)
                                                                                            CGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGG  <  SRR4302534.794408/94‑1 (MQ=60)
                                                                                            CGCGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGG  <  SRR4302534.619870/94‑1 (MQ=60)
                                                                                             |                                                                                            
CTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTACCTTCTCACTCACACTGGG  >  NC_000913/1512430‑1512615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑