Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,520,878 A→G V71A (GTG→GCG)  b1451 ← putative outer membrane receptor for iron transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,8780AG100.0% 775.3 / NA 224V71A (GTG→GCG) b1451putative outer membrane receptor for iron transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (102/122);  total (102/122)

Only 100 of 227 total aligned reads displayed.

ACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCC  >  NC_000913/1520790‑1520967
                                                                                        |                                                                                         
accgTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGcc                                                                                       <  2:501004/93‑1 (MQ=255)
 ccgTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCg                                                                                      >  2:6388522/1‑93 (MQ=255)
  cgTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCgg                                                                                     <  1:4241769/93‑1 (MQ=255)
   gTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGt                                                                                    >  2:443187/1‑93 (MQ=255)
   gTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGt                                                                                    <  2:3556348/93‑1 (MQ=255)
   gTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGt                                                                                    <  2:1881878/93‑1 (MQ=255)
   gTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGt                                                                                    <  2:1552787/93‑1 (MQ=255)
    tAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTc                                                                                   <  2:3887834/93‑1 (MQ=255)
    tAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTc                                                                                   <  1:1597388/93‑1 (MQ=255)
    tAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTc                                                                                   >  1:296205/1‑93 (MQ=255)
       gTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGt                                                                                <  2:5835561/93‑1 (MQ=255)
        tGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTg                                                                               <  1:4743660/93‑1 (MQ=255)
         ggAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGa                                                                              >  1:1026880/1‑93 (MQ=255)
           aGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGAtt                                                                            >  2:6016439/1‑93 (MQ=255)
           aGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGAtt                                                                            >  2:3105656/1‑93 (MQ=255)
           aGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGAtt                                                                            <  2:5314077/93‑1 (MQ=255)
           aGCGGCAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTCCCTGCAAACCAGGCGCGCCGGTCAGTGAtt                                                                            <  1:3327295/93‑1 (MQ=255)
            gCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTc                                                                           <  1:1252559/93‑1 (MQ=255)
            gCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTc                                                                           >  1:1428341/1‑93 (MQ=255)
            gCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTc                                                                           <  1:5118108/93‑1 (MQ=255)
               ggAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGa                                                                        <  2:4342044/93‑1 (MQ=255)
                  gCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACaa                                                                     >  1:1779918/1‑93 (MQ=255)
                      aaTCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTa                                                                 <  2:619341/93‑1 (MQ=255)
                      aaTCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTa                                                                 >  1:4871464/1‑93 (MQ=255)
                       aTCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTaa                                                                >  2:3772487/1‑93 (MQ=255)
                       aTCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTaa                                                                <  1:3780918/93‑1 (MQ=255)
                       aTCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTaa                                                                <  2:5496129/93‑1 (MQ=255)
                        tCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGATAAGTTAAt                                                               <  1:6319442/93‑1 (MQ=255)
                          cgcgAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATgc                                                             <  1:3496662/93‑1 (MQ=255)
                          cgcgAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATgc                                                             >  1:2740353/1‑93 (MQ=255)
                           gcgAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATgcg                                                            >  1:5688671/1‑93 (MQ=255)
                            cgAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATgcgc                                                           <  1:525748/93‑1 (MQ=255)
                             gAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGcgcg                                                          <  2:5655577/93‑1 (MQ=255)
                              aaTCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCgg                                                         >  1:3023789/1‑93 (MQ=255)
                              aaTCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCgg                                                         >  2:5996973/1‑93 (MQ=255)
                              aaTCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCgg                                                         <  2:4705893/93‑1 (MQ=255)
                               aTCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGgt                                                        >  1:3082243/1‑93 (MQ=255)
                                 cGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGgtgt                                                      <  1:4074113/93‑1 (MQ=255)
                                    cAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGc                                                   >  1:3648323/1‑93 (MQ=255)
                                    cAGCTGCAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGc                                                   >  1:3992558/1‑93 (MQ=255)
                                     aGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGcc                                                  >  2:4624576/1‑93 (MQ=255)
                                     aGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGcc                                                  <  2:1238542/93‑1 (MQ=255)
                                     aGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGcc                                                  >  1:5059265/1‑93 (MQ=255)
                                     aGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGca                                                  >  2:1186338/1‑92 (MQ=255)
                                      gCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCa                                                 <  2:4520274/93‑1 (MQ=255)
                                       cTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAg                                                <  2:4790503/93‑1 (MQ=255)
                                       cTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGCGATTCGGACAAGTTAATGCGCGGTGTTGCCAg                                                >  2:4291848/1‑93 (MQ=255)
                                        tGTAAACCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAATCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAgg                                               <  1:5502737/93‑1 (MQ=255)
                                          tAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGgcg                                             <  1:2302661/93‑1 (MQ=255)
                                           aaaTCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGgcgc                                            <  1:3562201/93‑1 (MQ=255)
                                            aaTCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGgcgcg                                           >  1:2862650/1‑92 (MQ=255)
                                             aTCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGgcgcgt                                          >  2:5110728/1‑91 (MQ=255)
                                              tCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGtc                                         <  1:2999832/93‑1 (MQ=255)
                                              tCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGtc                                         <  1:4648289/93‑1 (MQ=255)
                                                 tGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTctcc                                      <  1:4273896/93‑1 (MQ=255)
                                                  gcgcATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTctcct                                     <  1:2814000/93‑1 (MQ=255)
                                                   cgcATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTctcctc                                    <  2:2565178/93‑1 (MQ=255)
                                                     cATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGc                                  <  1:2004206/93‑1 (MQ=255)
                                                      aTAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGcc                                 >  2:3518848/1‑93 (MQ=255)
                                                        aGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCAt                               >  1:3211783/1‑93 (MQ=255)
                                                         gTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATc                              >  2:2629518/1‑93 (MQ=255)
                                                         gTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATc                              >  1:586761/1‑93 (MQ=255)
                                                           tCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATcca                            >  2:3296008/1‑93 (MQ=255)
                                                              gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                         >  2:2128961/1‑93 (MQ=255)
                                                              gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                         <  1:4570871/93‑1 (MQ=255)
                                                              gCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATccacca                         <  1:739248/93‑1 (MQ=255)
                                                               ccGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCcaccac                        <  2:2240207/93‑1 (MQ=255)
                                                                 ggTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGc                      >  2:434025/1‑93 (MQ=255)
                                                                  gTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATGCACCACGCt                     >  2:1918750/1‑93 (MQ=255)
                                                                  gTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCt                     >  1:3841303/1‑93 (MQ=255)
                                                                  gTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCt                     <  1:1642179/93‑1 (MQ=255)
                                                                   ttttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCtt                    <  2:2390619/93‑1 (MQ=255)
                                                                    tttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTa                   <  1:612624/93‑1 (MQ=255)
                                                                     ttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAc                  <  2:831700/93‑1 (MQ=255)
                                                                     ttGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAc                  <  2:3936781/93‑1 (MQ=255)
                                                                       gTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActg                >  1:4986736/1‑93 (MQ=255)
                                                                       gTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActg                <  1:963926/93‑1 (MQ=255)
                                                                        tagcTGCGAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgc               <  1:4356738/90‑1 (MQ=255)
                                                                        tACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgc               <  1:1979377/93‑1 (MQ=255)
                                                                        tACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgc               >  1:5375190/1‑93 (MQ=255)
                                                                          ccTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACATGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctg             <  1:2887254/93‑1 (MQ=255)
                                                                           cTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgc            <  2:871015/93‑1 (MQ=255)
                                                                           cTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgc            <  1:2662237/93‑1 (MQ=255)
                                                                             gCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgctg          <  2:3008401/93‑1 (MQ=255)
                                                                             gCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgctg          >  1:4517842/1‑93 (MQ=255)
                                                                             gCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTActgctgctg          >  2:5533253/1‑93 (MQ=255)
                                                                               aaaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTggg        <  2:650372/93‑1 (MQ=255)
                                                                               aaaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTggg        <  1:6408633/93‑1 (MQ=255)
                                                                               aaaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTggg        <  1:2462071/93‑1 (MQ=255)
                                                                                aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgggg       <  2:1379730/93‑1 (MQ=255)
                                                                                aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgggg       >  1:2491928/1‑93 (MQ=255)
                                                                                aaCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTgggg       <  1:3148834/93‑1 (MQ=255)
                                                                                 aCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGt      <  2:2861881/93‑1 (MQ=255)
                                                                                  ccAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTa     <  1:3936778/93‑1 (MQ=255)
                                                                                  ccAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTa     <  1:4378693/93‑1 (MQ=255)
                                                                                    aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc   >  2:3015660/1‑93 (MQ=255)
                                                                                    aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc   >  1:238797/1‑93 (MQ=255)
                                                                                    aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc   >  2:280051/1‑93 (MQ=255)
                                                                                    aGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATc   >  1:185096/1‑93 (MQ=255)
                                                                                     ggCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATcc  >  1:1088032/1‑93 (MQ=255)
                                                                                        |                                                                                         
ACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCC  >  NC_000913/1520790‑1520967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTT  >  NC_000913/1520786‑1520971
                                                                                            |                                                                                             
GAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGC                                                                                              >  SRR4302534.3576682/1‑94 (MQ=60)
 AATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCG                                                                                             <  SRR4302534.1927407/94‑1 (MQ=60)
     CCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGG                                                                                         >  SRR4302534.3980708/1‑94 (MQ=60)
      CGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGT                                                                                        <  SRR4302534.4356147/94‑1 (MQ=60)
      CGTAAGTGGAGCGGCAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTCCCTGCAAACCAGGCGCGCCGGT                                                                                        <  SRR4302534.681110/94‑1 (MQ=60)
       GTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTC                                                                                       >  SRR4302534.303866/1‑94 (MQ=60)
       GTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTC                                                                                       >  SRR4302534.5607976/1‑94 (MQ=60)
       GTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTC                                                                                       <  SRR4302534.2010662/94‑1 (MQ=60)
        TAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCA                                                                                      >  SRR4302534.2941892/1‑94 (MQ=60)
        TAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCA                                                                                      <  SRR4302534.1639128/94‑1 (MQ=60)
        TAAGTGGAGCGGGAGCCAAATCCGAGCATCGACACCTGTAAATCTTGCGCATAGTTCTCCCGGTTTTGTACCTGCAACCCAGGGGAGCCGGTCA                                                                                      <  SRR4302534.5250596/94‑1 (MQ=60)
        AAAGTGAAGCGGTAGCCAAATCGGCAAATCGACAGCTGTAAATCTTGCGCATACTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCA                                                                                      <  SRR4302534.5819503/94‑1 (MQ=60)
         AAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAG                                                                                     >  SRR4302534.3064120/1‑94 (MQ=60)
          AGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGT                                                                                    >  SRR4302534.4767494/1‑94 (MQ=60)
           GTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTG                                                                                   <  SRR4302534.1595836/94‑1 (MQ=60)
            TGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGA                                                                                  <  SRR4302534.4872431/94‑1 (MQ=60)
            TGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGA                                                                                  >  SRR4302534.1053330/1‑94 (MQ=60)
             GGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGAT                                                                                 >  SRR4302534.3636136/1‑94 (MQ=60)
             GGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGAT                                                                                 >  SRR4302534.44797/1‑94 (MQ=60)
             GGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGAT                                                                                 >  SRR4302534.2821312/1‑94 (MQ=60)
               AGCGGGAGCTAAATCTGCGAATCGACAGCTGTAAATCTTGCGCTTAGTACTGCCGGTTTTGTACCTGCAAACAAGGCGCGCCGGTCAGTGATTC                                                                               <  SRR4302534.3764928/94‑1 (MQ=60)
               AGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTC                                                                               >  SRR4302534.1465932/1‑94 (MQ=60)
               AGCGGCAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTCCCTGCAAACCAGGCGCGCCGGTCAGTGATTC                                                                               <  SRR4302534.3416277/94‑1 (MQ=60)
                GCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCG                                                                              <  SRR4302534.1285569/94‑1 (MQ=60)
                GCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCG                                                                              <  SRR4302534.5256271/94‑1 (MQ=60)
                 CGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGG                                                                             >  SRR4302534.3837858/1‑94 (MQ=60)
                    GAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACA                                                                          >  SRR4302534.3915609/1‑94 (MQ=60)
                     AGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAA                                                                         >  SRR4302534.1826137/1‑94 (MQ=60)
                      GCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAG                                                                        <  SRR4302534.5894513/94‑1 (MQ=60)
                       CCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGT                                                                       <  SRR4302534.1910970/94‑1 (MQ=60)
                       CCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGT                                                                       <  SRR4302534.1899073/94‑1 (MQ=60)
                        CAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTT                                                                      <  SRR4302534.6357551/94‑1 (MQ=60)
                         AAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTA                                                                     <  SRR4302534.4647649/94‑1 (MQ=60)
                         AAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTA                                                                     >  SRR4302534.5003586/1‑94 (MQ=60)
                           ATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAAT                                                                   <  SRR4302534.3882436/94‑1 (MQ=60)
                            TCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGATAAGTTAATG                                                                  <  SRR4302534.6490217/94‑1 (MQ=60)
                            TCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATG                                                                  >  SRR4302534.131114/1‑94 (MQ=60)
                             CCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGC                                                                 <  SRR4302534.3100621/94‑1 (MQ=60)
                             CCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGC                                                                 >  SRR4302534.2812559/1‑94 (MQ=60)
                              CGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCG                                                                <  SRR4302534.3590692/94‑1 (MQ=60)
                              CGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCG                                                                >  SRR4302534.5842742/1‑94 (MQ=60)
                                CGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCG                                                              >  SRR4302534.3391107/1‑94 (MQ=60)
                                CGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCG                                                              >  SRR4302534.5490898/1‑94 (MQ=60)
                                CGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCG                                                              <  SRR4302534.296291/94‑1 (MQ=60)
                                CGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCG                                                              <  SRR4302534.538809/94‑1 (MQ=60)
                                CGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCG                                                              <  SRR4302534.4533207/94‑1 (MQ=60)
                                 GAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGG                                                             >  SRR4302534.3103024/1‑94 (MQ=60)
                                  AATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGT                                                            >  SRR4302534.2521525/1‑94 (MQ=60)
                                  AATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGT                                                            >  SRR4302534.3163229/1‑94 (MQ=60)
                                    TCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGT                                                          <  SRR4302534.5690157/94‑1 (MQ=60)
                                    TCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGT                                                          <  SRR4302534.2539906/94‑1 (MQ=60)
                                     CGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTT                                                         <  SRR4302534.4183452/94‑1 (MQ=60)
                                      GACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTG                                                        <  SRR4302534.3134516/94‑1 (MQ=60)
                                       ACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGC                                                       >  SRR4302534.3746364/1‑94 (MQ=60)
                                       ACAGCTGCAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGC                                                       >  SRR4302534.4099431/1‑94 (MQ=60)
                                        CAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCC                                                      >  SRR4302534.5195906/1‑94 (MQ=60)
                                        CAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGGTGCC                                                      >  SRR4302534.4560008/1‑94 (MQ=60)
                                         AGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGGGATTCGGACAAGTTAATGCGCGGAGTTGCCA                                                     >  SRR4302534.1227555/1‑94 (MQ=60)
                                          GCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAG                                                    <  SRR4302534.3245211/94‑1 (MQ=60)
                                           CTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGG                                                   >  SRR4302534.2977980/1‑94 (MQ=60)
                                           CTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGG                                                   >  SRR4302534.2524427/1‑94 (MQ=60)
                                            TGTAAACCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAATCCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGC                                                  <  SRR4302534.5651146/94‑1 (MQ=60)
                                             GTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCG                                                 >  SRR4302534.1071321/1‑94 (MQ=60)
                                              TAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGATAAGTTAATGCGCGGTGTTGCCAGGCGC                                                <  SRR4302534.2417105/94‑1 (MQ=60)
                                              TAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGC                                                <  SRR4302534.2363780/94‑1 (MQ=60)
                                               AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                               <  SRR4302534.3658031/94‑1 (MQ=60)
                                               AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                               <  SRR4302534.4357309/94‑1 (MQ=60)
                                               AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                               >  SRR4302534.2937835/1‑94 (MQ=60)
                                               AAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCG                                               >  SRR4302534.2763504/1‑94 (MQ=60)
                                                AATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGT                                              >  SRR4302534.3208548/1‑94 (MQ=60)
                                                  TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCT                                            <  SRR4302534.4774475/94‑1 (MQ=60)
                                                  TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCT                                            <  SRR4302534.3078289/94‑1 (MQ=60)
                                                  TCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGAGCGGTGTTGCCAGGCGCGTCT                                            >  SRR4302534.2705861/1‑94 (MQ=60)
                                                     TGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCT                                         <  SRR4302534.4389483/94‑1 (MQ=60)
                                                      GCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTC                                        <  SRR4302534.4856182/94‑1 (MQ=60)
                                                      GCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTC                                        <  SRR4302534.2887977/94‑1 (MQ=60)
                                                      GCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTC                                        <  SRR4302534.4956255/94‑1 (MQ=60)
                                                       CGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCAGACAAGgtaacgcgccgtggcgccaggcgcgactccccc                                       >  SRR4302534.5766224/1‑61 (MQ=60)
                                                         CATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCC                                     <  SRR4302534.2056656/94‑1 (MQ=60)
                                                         CATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCC                                     >  SRR4302534.2766129/1‑94 (MQ=60)
                                                          ATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCA                                    <  SRR4302534.3208666/94‑1 (MQ=60)
                                                          ATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCA                                    >  SRR4302534.2334963/1‑94 (MQ=60)
                                                           TAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCAT                                   >  SRR4302534.5806423/1‑94 (MQ=60)
                                                           TAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCAT                                   >  SRR4302534.3297007/1‑94 (MQ=60)
                                                            AGTTCTGCTGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATC                                  <  SRR4302534.16504/94‑1 (MQ=60)
                                                            AGTTCTGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATC                                  >  SRR4302534.601092/1‑94 (MQ=60)
                                                                 TGCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCA                             <  SRR4302534.4048065/94‑1 (MQ=60)
                                                                  GCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                            >  SRR4302534.3525606/1‑94 (MQ=60)
                                                                  GCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                            <  SRR4302534.757555/94‑1 (MQ=60)
                                                                  GCCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                            <  SRR4302534.4694798/94‑1 (MQ=60)
                                                                  GCCGGTTTTGTACATGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCAC                            >  SRR4302534.3927203/1‑94 (MQ=60)
                                                                   CCGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACG                           <  SRR4302534.1785806/94‑1 (MQ=60)
                                                                    CGGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGC                          >  SRR4302534.5020672/1‑94 (MQ=60)
                                                                     GGTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCT                         >  SRR4302534.3944486/1‑94 (MQ=60)
                                                                      GTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTT                        >  SRR4302534.3426235/1‑94 (MQ=60)
                                                                      GTTTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTT                        <  SRR4302534.1684999/94‑1 (MQ=60)
                                                                        TTTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTAC                      <  SRR4302534.627511/94‑1 (MQ=60)
                                                                         TTGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACT                     <  SRR4302534.2034426/94‑1 (MQ=60)
                                                                          TGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTG                    <  SRR4302534.6111473/94‑1 (MQ=60)
                                                                          TGTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTG                    >  SRR4302534.5121678/1‑94 (MQ=60)
                                                                           GTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGC                   >  SRR4302534.5520030/1‑94 (MQ=60)
                                                                           GTACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGC                   <  SRR4302534.988482/94‑1 (MQ=60)
                                                                            TAGCTGCGAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                  <  SRR4302534.4474307/94‑1 (MQ=60)
                                                                            TACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                  >  SRR4302534.4838834/1‑94 (MQ=60)
                                                                            TACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                  <  SRR4302534.4100462/94‑1 (MQ=60)
                                                                            TACCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCT                  <  SRR4302534.2031203/94‑1 (MQ=60)
                                                                              CCTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACATGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGC                <  SRR4302534.2963016/94‑1 (MQ=60)
                                                                               CTGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCT               <  SRR4302534.2732617/94‑1 (MQ=60)
                                                                                TGCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTG              >  SRR4302534.4640221/1‑94 (MQ=60)
                                                                                 GCAAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGG             <  SRR4302534.2909096/94‑1 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCCTCCACCACGCTTACTGCTGCTGCGG           >  SRR4302534.6552177/1‑94 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG           >  SRR4302534.2558129/1‑94 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG           <  SRR4302534.887209/94‑1 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG           <  SRR4302534.6581590/94‑1 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG           >  SRR4302534.646152/1‑94 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG           <  SRR4302534.4405738/94‑1 (MQ=60)
                                                                                   AAACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGG           <  SRR4302534.2527479/94‑1 (MQ=60)
                                                                                    AACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGT          <  SRR4302534.4189427/94‑1 (MQ=60)
                                                                                    AACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGT          <  SRR4302534.3231679/94‑1 (MQ=60)
                                                                                    AACCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGT          <  SRR4302534.5127351/94‑1 (MQ=60)
                                                                                      CCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAT        <  SRR4302534.4496721/94‑1 (MQ=60)
                                                                                      CCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAT        >  SRR4302534.4213/1‑94 (MQ=60)
                                                                                      CCAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTAT        <  SRR4302534.4042227/94‑1 (MQ=60)
                                                                                       CAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATC       >  SRR4302534.189960/1‑94 (MQ=60)
                                                                                       CAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATC       >  SRR4302534.245048/1‑94 (MQ=60)
                                                                                       CAGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATC       <  SRR4302534.1506486/94‑1 (MQ=60)
                                                                                        AGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCC      <  SRR4302534.3738620/94‑1 (MQ=60)
                                                                                        AGGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCC      >  SRR4302534.1116333/1‑94 (MQ=60)
                                                                                         GGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCA     <  SRR4302534.525522/94‑1 (MQ=60)
                                                                                         GGCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCA     <  SRR4302534.5207601/94‑1 (MQ=60)
                                                                                          GCGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAG    >  SRR4302534.3687159/1‑94 (MQ=60)
                                                                                           CGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGT   >  SRR4302534.5152951/1‑94 (MQ=60)
                                                                                           CGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGT   <  SRR4302534.1963015/94‑1 (MQ=60)
                                                                                           CGCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGT   <  SRR4302534.5538546/94‑1 (MQ=60)
                                                                                            GCGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCGTCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTT  >  SRR4302534.4695805/1‑94 (MQ=60)
                                                                                            |                                                                                             
GAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGATTCGGACAAGTTAATGCGCGGTGTTGCCAGGCGCATCTCCTCGCCATCCACCACGCTTACTGCTGCTGGGGTATCCAGTT  >  NC_000913/1520786‑1520971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑