Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,626 T→C E180E (GAA→GAG)  rspA ← starvation sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,6260TC99.6% 791.6 / ‑4.8 226E180E (GAA→GAG) rspAstarvation sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (117/108);  minor base G (1/0);  total (118/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 247 total aligned reads displayed.

CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715
                                                                                          |                                                                                         
cagcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctc                                                                                         <  1:5971409/93‑1 (MQ=255)
 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        >  1:1987428/1‑93 (MQ=255)
 agcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctct                                                                                        <  2:6018109/93‑1 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       >  1:2165576/1‑93 (MQ=255)
  gcaAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctctt                                                                                       <  2:2996811/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAGCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  2:3969183/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  1:1645059/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  1:1670574/93‑1 (MQ=255)
   caAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGctcttc                                                                                      <  2:27443/93‑1 (MQ=255)
     aaTGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCg                                                                                    >  1:3692342/1‑93 (MQ=255)
       tGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGc                                                                                  <  2:489404/93‑1 (MQ=255)
          tCATTAAAACAAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCCTCGACCACAGCTGCTCTTCCGGCCAc                                                                               >  1:1880632/1‑93 (MQ=255)
           taTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                              <  2:4507754/92‑1 (MQ=255)
           cATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACt                                                                              <  2:3552082/93‑1 (MQ=255)
            aTTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTg                                                                             >  1:3203700/1‑93 (MQ=255)
             ttAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGt                                                                            >  1:4753829/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        >  1:3277963/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        >  2:3857917/1‑93 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        <  2:3581843/93‑1 (MQ=255)
                 aaCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTATCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCtt                                                                        <  2:210778/93‑1 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       <  2:1851745/93‑1 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       <  2:1486290/93‑1 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       >  2:5845477/1‑93 (MQ=255)
                  aCCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCttt                                                                       >  2:857469/1‑93 (MQ=255)
                   ccAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTg                                                                      <  2:1115151/93‑1 (MQ=255)
                    cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                                     <  2:2225653/93‑1 (MQ=255)
                    cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                                     >  2:53206/1‑93 (MQ=255)
                    cAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTgg                                                                     >  1:1188180/1‑93 (MQ=255)
                      aaCTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGtt                                                                   <  2:593425/93‑1 (MQ=255)
                         ttGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCg                                                                <  2:5583659/93‑1 (MQ=255)
                           gTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCggg                                                              <  2:4859763/93‑1 (MQ=255)
                              aCGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTc                                                           >  1:2354067/1‑93 (MQ=255)
                               cGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCa                                                          >  2:5012953/1‑93 (MQ=255)
                                gTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAt                                                         <  2:3351962/93‑1 (MQ=255)
                                  aCCGCGTCAAGCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       <  2:5234792/93‑1 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       >  2:3080163/1‑93 (MQ=255)
                                  aCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       >  1:343866/1‑93 (MQ=255)
                                  aCCGCGTCAAACAATATCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATaa                                                       <  2:6223521/93‑1 (MQ=255)
                                    cgcgTCAAACAATTTCGGCATGAAATCGAGGTATTACTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGc                                                     <  1:6230845/93‑1 (MQ=255)
                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    <  2:2620110/93‑1 (MQ=255)
                                     gcgTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGcc                                                    >  2:4554457/1‑93 (MQ=255)
                                      cgTCAAGCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCTTAAGCCa                                                   <  1:6131299/93‑1 (MQ=255)
                                       gTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTACTGCCACTGTCCTTTGGTTGCGGGCTCATAAGCCAg                                                  >  2:3947026/1‑93 (MQ=255)
                                         cAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGac                                                <  1:2615964/93‑1 (MQ=255)
                                          aaaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacc                                               >  2:5156069/1‑93 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                              >  1:2983196/1‑93 (MQ=255)
                                           aaCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacct                                              <  2:129741/93‑1 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:3776660/1‑93 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:5543173/1‑93 (MQ=255)
                                            aCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctt                                             >  2:1541104/1‑93 (MQ=255)
                                             cAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttt                                            <  1:1462576/93‑1 (MQ=255)
                                              aaTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGaccttta                                           >  1:4174744/1‑93 (MQ=255)
                                               aTTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttac                                          >  2:1259521/1‑93 (MQ=255)
                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                        <  1:1798339/93‑1 (MQ=255)
                                                 ttCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacct                                        <  2:2511845/93‑1 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       >  1:872009/1‑93 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       <  1:2047558/93‑1 (MQ=255)
                                                  tCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGacctttacctt                                       <  2:3686084/93‑1 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGGTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     >  1:1305842/1‑93 (MQ=255)
                                                    ggCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTc                                     >  2:1030277/1‑93 (MQ=255)
                                                     gCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCg                                    >  1:1098881/1‑93 (MQ=255)
                                                      cATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGa                                   >  2:5310747/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  1:3568041/93‑1 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  2:3493913/93‑1 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 >  1:5104310/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 >  1:6037429/1‑93 (MQ=255)
                                                        tGAAATCGAGGTATTTCCCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACa                                 <  1:5013132/93‑1 (MQ=255)
                                                         gAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAt                                <  1:1582909/93‑1 (MQ=255)
                                                           aaTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGCAATGc                              >  1:5583750/1‑93 (MQ=255)
                                                            aTCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGcc                             >  2:4252517/1‑93 (MQ=255)
                                                               gAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTa                          >  2:6081782/1‑93 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         >  2:429258/1‑93 (MQ=255)
                                                                aGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAg                         >  1:4431512/1‑93 (MQ=255)
                                                                  gTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggt                       <  1:1763494/93‑1 (MQ=255)
                                                                   tATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGGTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtg                      >  1:5504050/1‑93 (MQ=255)
                                                                    aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg                     <  2:1003471/93‑1 (MQ=255)
                                                                    aTTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtgg                     >  2:3380361/1‑93 (MQ=255)
                                                                     tttCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAggtggt                    <  1:28138/93‑1 (MQ=255)
                                                                      ttttcCGTCGACCACAGCTGCTTTTCCGGCCACTGTCCTTTGGTTGCGGGATCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGtt                   <  2:3442183/90‑1 (MQ=255)
                                                                       tctcCGTCGACCCCAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGttt                  <  1:4502570/93‑1 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                <  1:2841165/93‑1 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                >  1:1060598/1‑93 (MQ=255)
                                                                         tcCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTc                >  2:6178156/1‑93 (MQ=255)
                                                                          ccGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCa               >  2:2044873/1‑93 (MQ=255)
                                                                           cGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAt              <  1:4009585/93‑1 (MQ=255)
                                                                            gTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATa             >  1:5290578/1‑93 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            <  1:3653255/93‑1 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            <  1:4563912/93‑1 (MQ=255)
                                                                             tCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAc            >  1:4672640/1‑93 (MQ=255)
                                                                              cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAcc           >  1:813122/1‑93 (MQ=255)
                                                                              cGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGATCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAca           >  1:4244983/1‑92 (MQ=255)
                                                                                aCCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAg         >  1:5684881/1‑93 (MQ=255)
                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        >  2:469363/1‑93 (MQ=255)
                                                                                 ccACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        <  2:2835939/93‑1 (MQ=255)
                                                                                 ccACAGCTCCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAgg        <  2:437789/93‑1 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGa      >  1:3053226/1‑93 (MQ=255)
                                                                                   acaGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGa      <  2:5155130/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt    <  2:5210008/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt    <  1:3873978/93‑1 (MQ=255)
                                                                                     aGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGAtt    >  2:6287099/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCC  >  NC_000913/1652536‑1652715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  NC_000913/1652535‑1652719
                                                                                           |                                                                                             
GCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTC                                                                                             <  SRR4302534.1495804/94‑1 (MQ=60)
GCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTC                                                                                             <  SRR4302534.1218008/94‑1 (MQ=60)
GCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTC                                                                                             <  SRR4302534.2036510/94‑1 (MQ=60)
 CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCT                                                                                            <  SRR4302534.6133590/94‑1 (MQ=60)
 CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCT                                                                                            >  SRR4302534.2039471/1‑94 (MQ=60)
 CAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCT                                                                                            >  SRR4302534.1287463/1‑94 (MQ=60)
  AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                           >  SRR4302534.2222778/1‑94 (MQ=60)
  AGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTT                                                                                           <  SRR4302534.5131186/94‑1 (MQ=60)
   GCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTC                                                                                          >  SRR4302534.1265948/1‑94 (MQ=60)
    CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                         <  SRR4302534.3718991/94‑1 (MQ=60)
    CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                         <  SRR4302534.1714057/94‑1 (MQ=60)
    CAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCC                                                                                         <  SRR4302534.1687943/94‑1 (MQ=60)
     AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                        >  SRR4302534.3791628/1‑94 (MQ=60)
     AAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCG                                                                                        >  SRR4302534.1096117/1‑94 (MQ=60)
      AATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGG                                                                                       <  SRR4302534.4625794/94‑1 (MQ=60)
      AATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGG                                                                                       <  SRR4302534.5258543/94‑1 (MQ=60)
       ATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      <  SRR4302534.5993227/94‑1 (MQ=60)
       ATGTTCATTAAAACCAAACTTGATACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGC                                                                                      >  SRR4302534.4201863/1‑94 (MQ=60)
          TTCATTAAAACAAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCCTCGACCACAGCTGCTCTTCCGGCCAC                                                                                   >  SRR4302534.1929621/1‑94 (MQ=60)
           TCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACT                                                                                  >  SRR4302534.181208/1‑94 (MQ=60)
            CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 <  SRR4302534.1520733/94‑1 (MQ=60)
            CATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTG                                                                                 >  SRR4302534.3288651/1‑94 (MQ=60)
             ATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGT                                                                                <  SRR4302534.6159402/94‑1 (MQ=60)
             ATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGT                                                                                >  SRR4302534.4882881/1‑94 (MQ=60)
             ATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGT                                                                                >  SRR4302534.4296436/1‑94 (MQ=60)
               TAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCC                                                                              <  SRR4302534.6560459/94‑1 (MQ=60)
               TAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCC                                                                              >  SRR4302534.2337805/1‑94 (MQ=60)
                 AAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTT                                                                            >  SRR4302534.3365323/1‑94 (MQ=60)
                  AACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTT                                                                           >  SRR4302534.3816580/1‑94 (MQ=60)
                   ACCAAACTTGTTACGTCCCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGTCCACTGTCCTTTG                                                                          <  SRR4302534.3082332/94‑1 (MQ=60)
                    CCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGG                                                                         >  SRR4302534.1219425/1‑94 (MQ=60)
                    CCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGG                                                                         <  SRR4302534.442507/94‑1 (MQ=60)
                      AAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTT                                                                       >  SRR4302534.266985/1‑94 (MQ=60)
                        ACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGC                                                                     >  SRR4302534.3495534/1‑94 (MQ=60)
                         CTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCG                                                                    <  SRR4302534.3534106/94‑1 (MQ=60)
                          TTTTTACGTCCCGCGTAAAACAATTTCGGCATGAAAGCGAGGTATTTCTCGGTCGACCACAGCTGCTCTCCCGGCCACTGTCCTTTGGTTGCGG                                                                   <  SRR4302534.6075954/94‑1 (MQ=60)
                              TACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTC                                                               >  SRR4302534.2416540/1‑94 (MQ=60)
                                CGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCAT                                                             <  SRR4302534.2913847/94‑1 (MQ=60)
                                  TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                           >  SRR4302534.352668/1‑94 (MQ=60)
                                  TACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAA                                                           >  SRR4302534.1350198/1‑94 (MQ=60)
                                   ACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAG                                                          >  SRR4302534.811843/1‑94 (MQ=60)
                                    CCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGC                                                         >  SRR4302534.1131436/1‑94 (MQ=60)
                                     CGCGTCAAACAATTTCGGCATGAAATCGAGGTATTACTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCC                                                        <  SRR4302534.6399984/94‑1 (MQ=60)
                                     CGCGTCAAACAATTTCGGCATGAAATCGAGGCATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGCTCATAAGCC                                                        >  SRR4302534.2096875/1‑94 (MQ=60)
                                       CGTCAAGCAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCTTAAGCCAG                                                      <  SRR4302534.6298084/94‑1 (MQ=60)
                                          CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACC                                                   <  SRR4302534.2685189/94‑1 (MQ=60)
                                          CAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCATCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGAAC                                                   >  SRR4302534.5663537/1‑94 (MQ=60)
                                           AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  <  SRR4302534.4043838/94‑1 (MQ=60)
                                           AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  <  SRR4302534.1576280/94‑1 (MQ=60)
                                           AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  >  SRR4302534.3061240/1‑94 (MQ=60)
                                           AAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCT                                                  >  SRR4302534.1985179/1‑94 (MQ=60)
                                            AACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTT                                                 <  SRR4302534.1932996/94‑1 (MQ=60)
                                             ACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTT                                                <  SRR4302534.3172452/94‑1 (MQ=60)
                                              CAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTA                                               <  SRR4302534.1501030/94‑1 (MQ=60)
                                              CAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTA                                               <  SRR4302534.3327834/94‑1 (MQ=60)
                                              CAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTA                                               >  SRR4302534.4287511/1‑94 (MQ=60)
                                               AATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTAC                                              >  SRR4302534.4833087/1‑94 (MQ=60)
                                                  TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           <  SRR4302534.1845038/94‑1 (MQ=60)
                                                  TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           <  SRR4302534.6286349/94‑1 (MQ=60)
                                                  TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           >  SRR4302534.4613295/1‑94 (MQ=60)
                                                  TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           <  SRR4302534.4375588/94‑1 (MQ=60)
                                                  TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           >  SRR4302534.894039/1‑94 (MQ=60)
                                                  TTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTT                                           >  SRR4302534.171384/1‑94 (MQ=60)
                                                   TCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTT                                          <  SRR4302534.2101067/94‑1 (MQ=60)
                                                   TCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTT                                          <  SRR4302534.2531465/94‑1 (MQ=60)
                                                   TCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTT                                          >  SRR4302534.3006730/1‑94 (MQ=60)
                                                    CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGGTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302534.1340284/1‑94 (MQ=60)
                                                    CGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTC                                         >  SRR4302534.2207300/1‑94 (MQ=60)
                                                     GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                        >  SRR4302534.1127504/1‑94 (MQ=60)
                                                     GGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCG                                        <  SRR4302534.4184651/94‑1 (MQ=60)
                                                       CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      >  SRR4302534.6267195/1‑94 (MQ=60)
                                                       CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      <  SRR4302534.5048788/94‑1 (MQ=60)
                                                       CATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGAC                                      >  SRR4302534.636612/1‑94 (MQ=60)
                                                        ATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACA                                     >  SRR4302534.5242070/1‑94 (MQ=60)
                                                        ATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACA                                     >  SRR4302534.6201505/1‑94 (MQ=60)
                                                         TGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                    <  SRR4302534.3664016/94‑1 (MQ=60)
                                                         TGAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                    <  SRR4302534.1689948/94‑1 (MQ=60)
                                                         TGAAATCGAGGTATTTCCCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACAT                                    <  SRR4302534.5148626/94‑1 (MQ=60)
                                                          GAAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATG                                   <  SRR4302534.1624311/94‑1 (MQ=60)
                                                           AAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGCAATGC                                  >  SRR4302534.5734628/1‑94 (MQ=60)
                                                           AAATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGC                                  >  SRR4302534.5273487/1‑94 (MQ=60)
                                                             ATCGAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCG                                <  SRR4302534.5597595/94‑1 (MQ=60)
                                                                GAGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAG                             >  SRR4302534.4551171/1‑94 (MQ=60)
                                                                 AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                            >  SRR4302534.4701405/1‑94 (MQ=60)
                                                                 AGGTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGG                            <  SRR4302534.2117006/94‑1 (MQ=60)
                                                                   GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          <  SRR4302534.1809252/94‑1 (MQ=60)
                                                                   GTATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGGTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTG                          >  SRR4302534.5652488/1‑94 (MQ=60)
                                                                    TATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGG                         >  SRR4302534.950292/1‑94 (MQ=60)
                                                                    TATTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGG                         >  SRR4302534.1841558/1‑94 (MQ=60)
                                                                      TTTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTT                       <  SRR4302534.28901/94‑1 (MQ=60)
                                                                       TTCTCTGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTT                      <  SRR4302534.5182135/94‑1 (MQ=60)
                                                                       TTCTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGGTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTT                      >  SRR4302534.6109799/1‑94 (MQ=60)
                                                                        TCTCCGTCGACCCCAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTT                     <  SRR4302534.4624437/94‑1 (MQ=60)
                                                                         CTCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTC                    >  SRR4302534.1088087/1‑94 (MQ=60)
                                                                          TCCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCA                   <  SRR4302534.2915818/94‑1 (MQ=60)
                                                                           CCGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCAT                  >  SRR4302534.2938078/1‑94 (MQ=60)
                                                                            CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                 <  SRR4302534.850204/94‑1 (MQ=60)
                                                                            CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                 <  SRR4302534.4116956/94‑1 (MQ=60)
                                                                            CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                 >  SRR4302534.5433374/1‑94 (MQ=60)
                                                                            CGTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCAAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATA                 <  SRR4302534.3260329/94‑1 (MQ=60)
                                                                             GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTCCCTTTCGACATGCCGTAGGTGGTTTTCATAC                <  SRR4302534.3915306/94‑1 (MQ=60)
                                                                             GTCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATAC                >  SRR4302534.4799473/1‑94 (MQ=60)
                                                                              TCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACC               >  SRR4302534.833557/1‑94 (MQ=60)
                                                                              TCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACC               <  SRR4302534.4687645/94‑1 (MQ=60)
                                                                              TCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACC               <  SRR4302534.3751427/94‑1 (MQ=60)
                                                                              TCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGAGGTTTTCATACC               >  SRR4302534.4671961/1‑94 (MQ=60)
                                                                              TCGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGATCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACA               >  SRR4302534.4359472/1‑94 (MQ=60)
                                                                               CGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCA              >  SRR4302534.3712869/1‑94 (MQ=60)
                                                                               CGACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCA              >  SRR4302534.5686374/1‑94 (MQ=60)
                                                                                GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302534.1857875/1‑94 (MQ=60)
                                                                                GACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAG             >  SRR4302534.5838824/1‑94 (MQ=60)
                                                                                 ACCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGG            >  SRR4302534.2768434/1‑94 (MQ=60)
                                                                                  CCACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGG           >  SRR4302534.3768920/1‑94 (MQ=60)
                                                                                   CACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGA          <  SRR4302534.1747450/94‑1 (MQ=60)
                                                                                   CACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGA          >  SRR4302534.1659525/1‑94 (MQ=60)
                                                                                   CACAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGA          >  SRR4302534.3133331/1‑94 (MQ=60)
                                                                                     CAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATT        <  SRR4302534.3385618/94‑1 (MQ=60)
                                                                                     CAGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATT        >  SRR4302534.5778098/1‑94 (MQ=60)
                                                                                      AGCTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTC       <  SRR4302534.3977991/94‑1 (MQ=60)
                                                                                        CTGCTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCG     >  SRR4302534.1435802/1‑94 (MQ=60)
                                                                                           CTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  SRR4302534.3654240/1‑94 (MQ=60)
                                                                                           CTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  <  SRR4302534.6041092/94‑1 (MQ=60)
                                                                                           |                                                                                             
GCAGCAAATGTTCATTAAAACCAAACTTGTTACGTACCGCGTCAAACAATTTCGGCATGAAATCGAGGTATTTCTCCGTCGACCACAGCTGTTCTTCCGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCAC  >  NC_000913/1652535‑1652719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑