Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,718,191 A→G T98A (ACT→GCT)  slyB → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,718,1910AG99.5% 662.4 / NA 188T98A (ACT→GCT) slyBputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (83/104);  total (83/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 192 total aligned reads displayed.

GCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA  >  NC_000913/1718101‑1718280
                                                                                          |                                                                                         
 cTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTg                                                                                        <  1:4489463/93‑1 (MQ=255)
 cTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTg                                                                                        >  1:1118518/1‑93 (MQ=255)
 cTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTg                                                                                        <  2:5251104/93‑1 (MQ=255)
 cTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTg                                                                                        <  2:4905518/93‑1 (MQ=255)
  tgtgCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       <  2:5885180/93‑1 (MQ=255)
  tGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTgg                                                                                       >  2:1735863/1‑93 (MQ=255)
   gTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTGGCTGGt                                                                                      >  1:3534111/1‑93 (MQ=255)
   gTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGt                                                                                      >  2:1280006/1‑93 (MQ=255)
    ttCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc                                                                                     >  2:5991075/1‑93 (MQ=255)
    ttCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc                                                                                     >  2:1040124/1‑93 (MQ=255)
    ttCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTc                                                                                     <  1:2509624/93‑1 (MQ=255)
     tCTTGGTGGTTTCCTGGGGAATGCTGTTGGTGGCCGAACCGGGCGTCCTATGCCTGCTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCa                                                                                    <  1:4754597/93‑1 (MQ=255)
     tCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCa                                                                                    >  2:3570941/1‑93 (MQ=255)
      cTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAg                                                                                   <  2:5688403/93‑1 (MQ=255)
       ttGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAgg                                                                                  >  2:4729371/1‑93 (MQ=255)
       ttGGTGGTGTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAgg                                                                                  <  1:208677/93‑1 (MQ=255)
         tgtggtgTCGTGGGGAATACTGTTGGTGGCCGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc                                                                                <  1:394380/92‑1 (MQ=255)
         ggtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc                                                                                <  1:708864/93‑1 (MQ=255)
         ggtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc                                                                                >  1:841651/1‑93 (MQ=255)
         ggtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGc                                                                                <  1:1872283/93‑1 (MQ=255)
          gtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCg                                                                               >  1:4604569/1‑93 (MQ=255)
            ggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTa                                                                             <  2:260935/93‑1 (MQ=255)
             gtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAc                                                                            >  1:3195211/1‑93 (MQ=255)
             gtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAc                                                                            <  2:5042060/93‑1 (MQ=255)
              tttCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACa                                                                           >  2:873559/1‑93 (MQ=255)
                tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga                                                                         <  1:789871/93‑1 (MQ=255)
                tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga                                                                         >  1:2765728/1‑93 (MQ=255)
                tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACaga                                                                         <  2:1768501/93‑1 (MQ=255)
                 ccTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACagag                                                                        <  2:4206720/93‑1 (MQ=255)
                  cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACagagg                                                                       >  2:21001/1‑92 (MQ=255)
                  cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGt                                                                       >  2:3328850/1‑93 (MQ=255)
                  cTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGt                                                                       <  1:1400414/93‑1 (MQ=255)
                    ggggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGc                                                                     >  1:5694594/1‑93 (MQ=255)
                      ggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCaa                                                                   <  1:1940148/93‑1 (MQ=255)
                       gAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAAt                                                                  <  2:5445996/93‑1 (MQ=255)
                       gAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAAt                                                                  >  2:4816260/1‑93 (MQ=255)
                        aaTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATg                                                                 >  1:4003636/1‑93 (MQ=255)
                            atGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACa                                                             <  1:5201039/92‑1 (MQ=255)
                                  gtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGc                                                       <  1:2392505/93‑1 (MQ=255)
                                   tggCGGAGCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCa                                                      <  1:745967/93‑1 (MQ=255)
                                   tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCa                                                      <  1:545276/93‑1 (MQ=255)
                                    ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg                                                     <  1:5312910/93‑1 (MQ=255)
                                    ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAg                                                     >  1:262423/1‑93 (MQ=255)
                                     gCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAgg                                                    >  1:1505573/1‑93 (MQ=255)
                                     gCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAgg                                                    >  2:4854022/1‑93 (MQ=255)
                                      cGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAggg                                                   <  1:3364472/93‑1 (MQ=255)
                                       ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgt                                                  <  2:2395411/93‑1 (MQ=255)
                                        gAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGgtg                                                 <  1:5635129/93‑1 (MQ=255)
                                          aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTc                                               <  2:4059830/93‑1 (MQ=255)
                                           ccGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCg                                              <  1:4359729/93‑1 (MQ=255)
                                            cGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                             >  1:167285/1‑93 (MQ=255)
                                             gggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAg                                            <  2:1113802/93‑1 (MQ=255)
                                              ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGc                                           >  2:44572/1‑93 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCGGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          >  2:3012210/1‑93 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          >  1:4083028/1‑93 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          <  2:3559945/93‑1 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                          >  1:4083029/1‑93 (MQ=255)
                                               gCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCAATGAACAAAACGCAGGGTGTCGAgtt                                          >  1:5369428/1‑91 (MQ=255)
                                                 gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTgg                                        >  1:2715317/1‑93 (MQ=255)
                                                  ttCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa                                       >  1:4796895/1‑93 (MQ=255)
                                                  ttCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGa                                       >  2:2525676/1‑93 (MQ=255)
                                                    ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa                                     >  1:858610/1‑93 (MQ=255)
                                                    ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa                                     <  1:2332690/93‑1 (MQ=255)
                                                      ctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAtt                                   <  1:4895612/93‑1 (MQ=255)
                                                      ctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAtt                                   >  1:4243284/1‑93 (MQ=255)
                                                       tGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTc                                  <  2:4345328/93‑1 (MQ=255)
                                                          cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               <  2:295270/93‑1 (MQ=255)
                                                          cTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                               >  1:771648/1‑93 (MQ=255)
                                                           tACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaa                              <  1:1819257/93‑1 (MQ=255)
                                                           tACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaa                              >  1:4193065/1‑93 (MQ=255)
                                                           tACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaa                              <  2:3779588/93‑1 (MQ=255)
                                                           tACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaa                              >  2:4141155/1‑93 (MQ=255)
                                                            aCTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaaa                             >  2:160045/1‑93 (MQ=255)
                                                            aCTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTaaa                             >  1:5030717/1‑93 (MQ=255)
                                                              tGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGa                           <  1:4686719/93‑1 (MQ=255)
                                                               gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                          <  2:2056144/93‑1 (MQ=255)
                                                               gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                          <  1:5218327/93‑1 (MQ=255)
                                                                cagcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACg                         <  2:5463244/93‑1 (MQ=255)
                                                                cagcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACg                         <  1:3043688/93‑1 (MQ=255)
                                                                 agcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGa                        <  2:4642917/93‑1 (MQ=255)
                                                                  gcagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAt                       >  1:2463400/1‑93 (MQ=255)
                                                                     ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATgga                    >  2:5214036/1‑92 (MQ=255)
                                                                      gcgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTa                   <  1:5428961/93‑1 (MQ=255)
                                                                       cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTaa                  <  2:2795331/93‑1 (MQ=255)
                                                                        gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 <  1:5763957/93‑1 (MQ=255)
                                                                        gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 <  2:3489841/93‑1 (MQ=255)
                                                                        gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAt                 <  2:1925887/93‑1 (MQ=255)
                                                                         cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATa                <  2:4575490/93‑1 (MQ=255)
                                                                         cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATa                <  1:1204396/93‑1 (MQ=255)
                                                                          tGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAc               <  2:6139651/93‑1 (MQ=255)
                                                                            ttGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACca             <  1:1018914/93‑1 (MQ=255)
                                                                              gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc           >  2:4689423/1‑93 (MQ=255)
                                                                              gCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc           <  1:5905696/93‑1 (MQ=255)
                                                                               cAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatca          <  1:5952363/93‑1 (MQ=255)
                                                                                aGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatcat         <  2:3098562/93‑1 (MQ=255)
                                                                                  gTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatcatag       <  1:1968366/93‑3 (MQ=255)
                                                                                  gTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtgg       >  2:2701367/1‑93 (MQ=255)
                                                                                   tGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggt      <  2:219178/93‑1 (MQ=255)
                                                                                    ggCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggtg     <  1:1002005/93‑1 (MQ=255)
                                                                                       gTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTa  >  1:966458/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA  >  NC_000913/1718101‑1718280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  >  NC_000913/1718098‑1718283
                                                                                             |                                                                                              
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAG                                                                                                <  SRR4302532.3267715/94‑1 (MQ=60)
 GTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGC                                                                                               <  SRR4302532.6133060/94‑1 (MQ=60)
  TGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCT                                                                                              >  SRR4302532.3042323/1‑94 (MQ=60)
   GCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTG                                                                                             >  SRR4302532.1155878/1‑94 (MQ=60)
    CTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGG                                                                                            <  SRR4302532.4642702/94‑1 (MQ=60)
     TGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTGGCTGGT                                                                                           >  SRR4302532.3652365/1‑94 (MQ=60)
       TTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCA                                                                                         <  SRR4302532.2593347/94‑1 (MQ=60)
        TCTTGGTGGTTTCCTGGGGAATGCTGTTGGTGGCCGAACCGGGCGTCCTATGCCTGCTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAG                                                                                        <  SRR4302532.4917202/94‑1 (MQ=60)
         CTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGG                                                                                       <  SRR4302532.2507890/94‑1 (MQ=60)
         CTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGG                                                                                       >  SRR4302532.2687990/1‑94 (MQ=60)
          TTGGTGGTGTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGG                                                                                      <  SRR4302532.215799/94‑1 (MQ=60)
           TGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGC                                                                                     >  SRR4302532.1528038/1‑94 (MQ=60)
           TGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGC                                                                                     >  SRR4302532.870545/1‑94 (MQ=60)
            tgtggtgtcgTGGGGAATACTGTTGGTGGCCGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCG                                                                                    <  SRR4302532.408182/84‑1 (MQ=60)
            GGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCG                                                                                    <  SRR4302532.1934290/94‑1 (MQ=60)
            GGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCG                                                                                    <  SRR4302532.733081/94‑1 (MQ=60)
            GGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCG                                                                                    >  SRR4302532.4761571/1‑94 (MQ=60)
              TGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTA                                                                                  >  SRR4302532.1898579/1‑94 (MQ=60)
               GGTTTCCTGGGGAATACTGTTGGTGTCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAC                                                                                 <  SRR4302532.3541965/94‑1 (MQ=60)
               GGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAC                                                                                 >  SRR4302532.3301304/1‑94 (MQ=60)
               GGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTAC                                                                                 >  SRR4302532.3253745/1‑94 (MQ=60)
                GTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACA                                                                                <  SRR4302532.6148925/94‑1 (MQ=60)
                  TTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGA                                                                              >  SRR4302532.2858594/1‑94 (MQ=60)
                   TCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAG                                                                             <  SRR4302532.816940/94‑1 (MQ=60)
                   TCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAG                                                                             <  SRR4302532.1439941/94‑1 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                            <  SRR4302532.5465718/94‑1 (MQ=60)
                    CCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGT                                                                            <  SRR4302532.852741/94‑1 (MQ=60)
                     CTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTG                                                                           <  SRR4302532.1447341/94‑1 (MQ=60)
                      TGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGC                                                                          >  SRR4302532.5893268/1‑94 (MQ=60)
                       GGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA                                                                         >  SRR4302532.3423488/1‑94 (MQ=60)
                         GGATTACTGTTGGCTGCGGAACCTGGCGTTCTCTGGCTACAGCAGCAGGCGCTGTTGCAGGTGGCTTAGCTGGCTAGGGCGCACAGAGTGCA‑‑AG                                                                     >  SRR4302532.5654549/1‑94 (MQ=42)
                         GGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑AT                                                                     <  SRR4302532.2004663/94‑1 (MQ=60)
                          GAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATG                                                                    >  SRR4302532.4138513/1‑94 (MQ=60)
                           AATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGA                                                                   >  SRR4302532.6331618/1‑94 (MQ=60)
                            ATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAA                                                                  >  SRR4302532.4369978/1‑94 (MQ=60)
                            ATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAA                                                                  <  SRR4302532.2714659/94‑1 (MQ=60)
                               ATGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAA                                                               <  SRR4302532.5380375/94‑1 (MQ=60)
                                TGTTGGTGGCGGAGCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAA                                                              <  SRR4302532.1771172/94‑1 (MQ=60)
                                 GTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAA                                                             <  SRR4302532.1017039/94‑1 (MQ=60)
                                    GGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGC                                                          <  SRR4302532.4860491/94‑1 (MQ=60)
                                     GTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCA                                                         <  SRR4302532.2472201/94‑1 (MQ=60)
                                      TGGCGGAGCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAG                                                        <  SRR4302532.771497/94‑1 (MQ=60)
                                      TGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAG                                                        <  SRR4302532.564276/94‑1 (MQ=60)
                                      TGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAG                                                        >  SRR4302532.271535/1‑94 (MQ=60)
                                      GGGCGGAACCGTGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACATAGTGCA‑‑ATGAACAAAACGCAG                                                        <  SRR4302532.1958599/94‑1 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGG                                                       >  SRR4302532.1556396/1‑94 (MQ=60)
                                       GGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGG                                                       <  SRR4302532.5496656/94‑1 (MQ=60)
                                         CGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGT                                                     <  SRR4302532.3476909/94‑1 (MQ=60)
                                          GGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTG                                                    >  SRR4302532.5130838/1‑94 (MQ=60)
                                          GGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTG                                                    <  SRR4302532.4258307/94‑1 (MQ=60)
                                           GAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGT                                                   <  SRR4302532.5830862/94‑1 (MQ=60)
                                              CCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGA                                                <  SRR4302532.4507444/94‑1 (MQ=60)
                                              CCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGA                                                >  SRR4302532.172893/1‑94 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAG                                               <  SRR4302532.1156991/94‑1 (MQ=60)
                                               CGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAG                                               >  SRR4302532.4705275/1‑94 (MQ=60)
                                                 GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCT                                             >  SRR4302532.4220106/1‑94 (MQ=60)
                                                 GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCT                                             >  SRR4302532.4220105/1‑94 (MQ=60)
                                                 GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCT                                             >  SRR4302532.4184688/1‑94 (MQ=60)
                                                 GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCT                                             <  SRR4302532.4108542/94‑1 (MQ=60)
                                                 GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGGGCA‑‑ATGAACAAAACGCAGGGTGTCGAGTT                                             >  SRR4302532.5555492/1‑94 (MQ=60)
                                                   CGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGG                                           >  SRR4302532.2126815/1‑94 (MQ=60)
                                                   CGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGG                                           >  SRR4302532.2806642/1‑94 (MQ=60)
                                                    GTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGA                                          <  SRR4302532.2575090/94‑1 (MQ=60)
                                                    GTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGA                                          >  SRR4302532.4962102/1‑94 (MQ=60)
                                                     TTCTCTGGCTACTGCAGGAGGCGCTGTTGCAGGCGGCGTAGCTGGTCAGGGCGTACAGA‑‑GCAACATGAACAAAACGCAGGGAGTCGAGCTGGCA                                         >  SRR4302532.4309190/1‑94 (MQ=60)
                                                      TCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAA                                        >  SRR4302532.888110/1‑94 (MQ=60)
                                                       CTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAAT                                       <  SRR4302532.5536612/94‑1 (MQ=60)
                                                       CTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAAT                                       <  SRR4302532.2410389/94‑1 (MQ=60)
                                                        TCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATT                                      >  SRR4302532.4385842/1‑94 (MQ=60)
                                                         CTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTC                                     >  SRR4302532.5911/1‑94 (MQ=60)
                                                         CTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTC                                     <  SRR4302532.6067511/94‑1 (MQ=60)
                                                         CTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTC                                     <  SRR4302532.5063863/94‑1 (MQ=60)
                                                          TGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCG                                    >  SRR4302532.461956/1‑94 (MQ=60)
                                                            GCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTA                                  >  SRR4302532.798273/1‑94 (MQ=60)
                                                             CTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAA                                 >  SRR4302532.4333773/1‑94 (MQ=60)
                                                              TACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAA                                >  SRR4302532.5203268/1‑94 (MQ=60)
                                                              TACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAA                                <  SRR4302532.1879898/94‑1 (MQ=60)
                                                              TACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAA                                <  SRR4302532.5630941/94‑1 (MQ=60)
                                                               ACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAG                               <  SRR4302532.1707218/94‑1 (MQ=60)
                                                                 TGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAC                             <  SRR4302532.4846566/94‑1 (MQ=60)
                                                                 TGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAC                             <  SRR4302532.5580648/94‑1 (MQ=60)
                                                                  GCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACG                            <  SRR4302532.522780/94‑1 (MQ=60)
                                                                  GCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACG                            <  SRR4302532.5398294/94‑1 (MQ=60)
                                                                   CAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGA                           <  SRR4302532.3144131/94‑1 (MQ=60)
                                                                    AGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAT                          >  SRR4302532.2545363/1‑94 (MQ=60)
                                                                    AGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGAT                          >  SRR4302532.1137316/1‑94 (MQ=60)
                                                                     GCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATG                         <  SRR4302532.4457486/94‑1 (MQ=60)
                                                                     GCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATG                         <  SRR4302532.2331581/94‑1 (MQ=60)
                                                                      CAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGG                        >  SRR4302532.878391/1‑94 (MQ=60)
                                                                         GCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAA                     <  SRR4302532.5617115/94‑1 (MQ=60)
                                                                          CGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAT                    <  SRR4302532.4999884/94‑1 (MQ=60)
                                                                          CGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAATGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAAT                    >  SRR4302532.5941119/1‑94 (MQ=60)
                                                                           GCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATA                   <  SRR4302532.5964543/94‑1 (MQ=60)
                                                                            CTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAC                  <  SRR4302532.1244692/94‑1 (MQ=60)
                                                                             TGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACC                 >  SRR4302532.4956082/1‑94 (MQ=60)
                                                                              GTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTATAGACGATGGTAATACCA                >  SRR4302532.890504/1‑94 (MQ=60)
                                                                               TTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCAT               <  SRR4302532.1053481/94‑1 (MQ=60)
                                                                                 GCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCA             <  SRR4302532.6110213/94‑1 (MQ=60)
                                                                                  CAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAT            >  SRR4302532.2857364/1‑94 (MQ=60)
                                                                                  CAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAT            <  SRR4302532.6158314/94‑1 (MQ=60)
                                                                                    GGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGG          <  SRR4302532.4814894/94‑1 (MQ=60)
                                                                                     GTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGT         <  SRR4302532.2033758/94‑1 (MQ=60)
                                                                                     GTGGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATAGT         <  SRR4302532.2033759/94‑1 (MQ=60)
                                                                                       GGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGG       <  SRR4302532.1036115/94‑1 (MQ=60)
                                                                                       GGCGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGG       >  SRR4302532.784836/1‑94 (MQ=60)
                                                                                         CGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA     <  SRR4302532.3731331/94‑1 (MQ=60)
                                                                                         CGTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTA     >  SRR4302532.999449/1‑94 (MQ=60)
                                                                                          GTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTAC    <  SRR4302532.756778/94‑1 (MQ=60)
                                                                                          GTAGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTAC    >  SRR4302532.3373837/1‑94 (MQ=60)
                                                                                            AGCTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  <  SRR4302532.1376620/94‑1 (MQ=60)
                                                                                             |                                                                                              
GGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAACTGGTCAGGGCGTACAGAGTGCA‑‑ATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAG  >  NC_000913/1718098‑1718283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑