Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,718,640 A→G L72P (CTG→CCG)  slyA ← SlyA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,718,6400AG86.3% 519.5 / 65.4 197L72P (CTG→CCG) slyASlyA
Reads supporting (aligned to +/- strand):  ref base A (14/13);  new base G (90/80);  total (104/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 199 total aligned reads displayed.

ATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATG  >  NC_000913/1718550‑1718728
                                                                                          |                                                                                        
  cAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTg                                                                                      <  2:6298670/93‑1 (MQ=255)
   aGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTgg                                                                                     >  1:483994/1‑93 (MQ=255)
    gCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGt                                                                                    >  2:5170478/1‑93 (MQ=255)
      ggCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTcc                                                                                  <  1:1238754/93‑1 (MQ=255)
       gCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCa                                                                                 <  1:6613332/93‑1 (MQ=255)
        ctctGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTGCag                                                                                >  2:855799/1‑93 (MQ=255)
        ctctGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCag                                                                                >  2:4775873/1‑93 (MQ=255)
          ctGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCagag                                                                              <  1:1471071/93‑1 (MQ=255)
            gCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTa                                                                            <  2:3042396/93‑1 (MQ=255)
            gCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTa                                                                            >  2:4434489/1‑93 (MQ=255)
            gCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTa                                                                            <  1:2550507/93‑1 (MQ=255)
             ccTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTAc                                                                           >  1:5999294/1‑93 (MQ=255)
              cTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACg                                                                          >  1:5148149/1‑93 (MQ=255)
               ttttCTGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACgg                                                                         <  2:3447214/93‑1 (MQ=255)
               ttttCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACgg                                                                         >  2:6163/1‑93 (MQ=255)
               ttttCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACgg                                                                         >  1:3808559/1‑93 (MQ=255)
                tttCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGa                                                                        >  1:5702163/1‑93 (MQ=255)
                 ttCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGAc                                                                       <  1:4343384/93‑1 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCa                                                                     >  1:1155092/1‑93 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCa                                                                     <  2:1392027/93‑1 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCa                                                                     >  1:675905/1‑93 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCa                                                                     >  2:5756577/1‑93 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCa                                                                     >  1:1844297/1‑93 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCa                                                                     <  1:6216855/93‑1 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCa                                                                     <  2:5745358/93‑1 (MQ=255)
                   ccGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTGTTCTTCCGGTTGGTCCAGAGTACGGACCa                                                                     >  1:2201101/1‑93 (MQ=255)
                    cGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAg                                                                    >  2:2578372/1‑93 (MQ=255)
                     gTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGt                                                                   <  2:2702061/93‑1 (MQ=255)
                     gTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGt                                                                   >  2:2916385/1‑93 (MQ=255)
                     gTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGt                                                                   <  2:4759718/93‑1 (MQ=255)
                      tCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTg                                                                  >  1:3954443/1‑93 (MQ=255)
                      tCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTg                                                                  >  2:6489702/1‑93 (MQ=255)
                      tCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTg                                                                  >  1:4534740/1‑93 (MQ=255)
                      tCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTg                                                                  <  2:4152954/93‑1 (MQ=255)
                       cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGa                                                                 <  2:665251/93‑1 (MQ=255)
                       cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGa                                                                 <  2:5035076/93‑1 (MQ=255)
                       cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGCTGGTCCAGAGTACGGACCAGTGa                                                                 >  1:1696244/1‑93 (MQ=255)
                       cAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGa                                                                 >  1:3628658/1‑93 (MQ=255)
                         gTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATg                                                               >  2:4289401/1‑93 (MQ=255)
                         gTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATg                                                               <  2:2675900/93‑1 (MQ=255)
                          tttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAGATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATgg                                                              >  2:6009880/1‑93 (MQ=255)
                          tttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATgg                                                              >  1:3830618/1‑93 (MQ=255)
                           ttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGgc                                                             >  2:1694989/1‑93 (MQ=255)
                           ttAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGgc                                                             <  1:437937/93‑1 (MQ=255)
                              aTACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGgctgc                                                          >  1:588406/1‑93 (MQ=255)
                              aTACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGgctgc                                                          <  1:4353231/93‑1 (MQ=255)
                               tACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGgctgct                                                         <  1:4776455/93‑1 (MQ=255)
                                  gTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGa                                                      <  1:1267161/93‑1 (MQ=255)
                                  gTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGa                                                      >  1:2377853/1‑93 (MQ=255)
                                   tttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGAt                                                     <  1:2110050/93‑1 (MQ=255)
                                   tttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGAt                                                     <  1:2874043/93‑1 (MQ=255)
                                    ttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATg                                                    >  2:27876/1‑93 (MQ=255)
                                    ttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATg                                                    <  1:5623296/93‑1 (MQ=255)
                                    ttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATg                                                    <  2:3165917/93‑1 (MQ=255)
                                    ttAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATg                                                    <  2:1543825/93‑1 (MQ=255)
                                          cgacgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATc                                              >  1:2247793/1‑93 (MQ=255)
                                            acgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGc                                            >  1:5223289/1‑93 (MQ=255)
                                            acgaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGc                                            <  2:2995137/93‑1 (MQ=255)
                                              gaTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtt                                          <  2:6566053/93‑1 (MQ=255)
                                               aTCGCTGGCACAAGTTTGATGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                         >  1:6495720/1‑93 (MQ=255)
                                               aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                         >  2:1869483/1‑93 (MQ=255)
                                               aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                         >  1:907216/1‑93 (MQ=255)
                                               aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                         >  2:5433077/1‑93 (MQ=255)
                                               aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                         <  1:2599417/93‑1 (MQ=255)
                                               aTCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCttt                                         <  1:5175970/93‑1 (MQ=255)
                                                tCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCtttt                                        <  2:1515370/93‑1 (MQ=255)
                                                   cTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcc                                     >  1:1979691/1‑93 (MQ=255)
                                                   cTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGcc                                     >  2:1030180/1‑93 (MQ=255)
                                                    tGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCa                                    >  1:4539189/1‑93 (MQ=255)
                                                    tGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCa                                    <  1:582417/93‑1 (MQ=255)
                                                    tGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCa                                    >  2:1692363/1‑93 (MQ=255)
                                                      gCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGt                                  >  2:4488888/1‑93 (MQ=255)
                                                        acaAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTg                                >  1:6255064/1‑93 (MQ=255)
                                                          aaGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGaa                              <  2:4231225/93‑1 (MQ=255)
                                                          aaGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGaa                              >  2:4875134/1‑93 (MQ=255)
                                                            gTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAtt                            >  2:2472690/1‑93 (MQ=255)
                                                            gTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAtt                            <  1:5812526/93‑1 (MQ=255)
                                                             tttGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAttt                           <  1:3370766/93‑1 (MQ=255)
                                                             tttGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAAttt                           <  1:3746906/93‑1 (MQ=255)
                                                                    cgAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTg                    <  1:4565960/93‑1 (MQ=255)
                                                                     gAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTgg                   >  2:3926843/1‑93 (MQ=255)
                                                                      aaaTTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGt                  >  1:2463942/1‑93 (MQ=255)
                                                                      aaaTTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGt                  <  1:4265396/93‑1 (MQ=255)
                                                                          tAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTgg              >  1:3486597/1‑93 (MQ=255)
                                                                            aCCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAg            >  1:5305323/1‑93 (MQ=255)
                                                                            aCCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAg            >  1:506462/1‑93 (MQ=255)
                                                                             cccTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAgg           <  2:1221766/93‑1 (MQ=255)
                                                                             cccTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAgg           >  2:4011344/1‑93 (MQ=255)
                                                                               cTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa         <  1:4136479/93‑1 (MQ=255)
                                                                               cTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTa         <  1:2944046/93‑1 (MQ=255)
                                                                                tttttCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTaa        >  1:1550322/1‑93 (MQ=255)
                                                                                tttttCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTaa        >  2:549188/1‑93 (MQ=255)
                                                                                tttttCTTCCGATTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTaa        <  1:1105642/93‑1 (MQ=255)
                                                                                 ttttCTTCCGGTTGGTCCAGAGTACGGCCCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAc       <  2:1275100/93‑1 (MQ=255)
                                                                                 ttttCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAc       <  2:4969975/93‑1 (MQ=255)
                                                                                 ttttCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAc       >  1:2712318/1‑93 (MQ=255)
                                                                                  tttCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACt      >  2:559512/1‑93 (MQ=255)
                                                                                  tttCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACt      <  2:2248498/93‑1 (MQ=255)
                                                                                     cttcCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGAt   <  2:2141915/93‑1 (MQ=255)
                                                                                      ttcCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATg  >  1:633610/1‑93 (MQ=255)
                                                                                          |                                                                                        
ATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATG  >  NC_000913/1718550‑1718728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATAT  >  NC_000913/1718547‑1718733
                                                                                             |                                                                                             
CTGATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCG                                                                                               <  SRR4302547.890188/94‑1 (MQ=60)
CTGATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCG                                                                                               >  SRR4302547.4216866/1‑94 (MQ=60)
CTGATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCG                                                                                               >  SRR4302547.4468079/1‑94 (MQ=60)
     CAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGG                                                                                          >  SRR4302547.492864/1‑94 (MQ=60)
     CAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGG                                                                                          >  SRR4302547.5280354/1‑94 (MQ=60)
      AGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGT                                                                                         >  SRR4302547.1474805/1‑94 (MQ=60)
         GGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCA                                                                                      <  SRR4302547.1263761/94‑1 (MQ=60)
          GCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAG                                                                                     <  SRR4302547.6750986/94‑1 (MQ=60)
             CTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGT                                                                                  <  SRR4302547.1500457/94‑1 (MQ=60)
             CTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGT                                                                                  <  SRR4302547.617154/94‑1 (MQ=60)
             CTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGT                                                                                  >  SRR4302547.3903784/1‑94 (MQ=60)
               GCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTAC                                                                                <  SRR4302547.2531063/94‑1 (MQ=60)
               GCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTAC                                                                                <  SRR4302547.1284168/94‑1 (MQ=60)
               GCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTAC                                                                                >  SRR4302547.6123800/1‑94 (MQ=60)
               GCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTAC                                                                                <  SRR4302547.2602011/94‑1 (MQ=60)
                CCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACG                                                                               >  SRR4302547.5254170/1‑94 (MQ=60)
                CCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACG                                                                               >  SRR4302547.208360/1‑94 (MQ=60)
                CCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACG                                                                               <  SRR4302547.2489531/94‑1 (MQ=60)
                 CTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGG                                                                              >  SRR4302547.4097167/1‑94 (MQ=60)
                 CTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGG                                                                              >  SRR4302547.3241853/1‑94 (MQ=60)
                 CTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGG                                                                              >  SRR4302547.3886188/1‑94 (MQ=60)
                  TTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGC                                                                             >  SRR4302547.4416902/1‑94 (MQ=60)
                  TTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGA                                                                             >  SRR4302547.5819952/1‑94 (MQ=60)
                  TTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACACTTTTTCTTCCGGTTGGGCCAGAGTACGGA                                                                             >  SRR4302547.4643999/1‑94 (MQ=60)
                    TTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACC                                                                           <  SRR4302547.4432678/94‑1 (MQ=60)
                     TCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCA                                                                          >  SRR4302547.1178464/1‑94 (MQ=60)
                     TCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCA                                                                          >  SRR4302547.688348/1‑94 (MQ=60)
                     TCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCA                                                                          >  SRR4302547.197245/1‑94 (MQ=60)
                     TCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCA                                                                          >  SRR4302547.1880731/1‑94 (MQ=60)
                     TCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTGTTCTTCCGGTTGGTCCAGAGTACGGACCA                                                                          >  SRR4302547.2245321/1‑94 (MQ=60)
                      CCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAG                                                                         <  SRR4302547.6345966/94‑1 (MQ=60)
                       CGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGT                                                                        >  SRR4302547.4252799/1‑94 (MQ=60)
                       CGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGT                                                                        >  SRR4302547.6690425/1‑94 (MQ=60)
                        GTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTG                                                                       <  SRR4302547.84599/94‑1 (MQ=60)
                        GTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTG                                                                       >  SRR4302547.4034877/1‑94 (MQ=60)
                        GTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTG                                                                       >  SRR4302547.4628457/1‑94 (MQ=60)
                        GTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTG                                                                       <  SRR4302547.1254872/94‑1 (MQ=60)
                         TCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGCTGGTCCAGAGTACGGACCAGTGA                                                                      >  SRR4302547.1729597/1‑94 (MQ=60)
                         TCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGA                                                                      >  SRR4302547.3702680/1‑94 (MQ=60)
                          CAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGAT                                                                     <  SRR4302547.5994109/94‑1 (MQ=60)
                           AGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATG                                                                    <  SRR4302547.2682891/94‑1 (MQ=60)
                            GTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGG                                                                   >  SRR4302547.3908642/1‑94 (MQ=60)
                            GTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGG                                                                   <  SRR4302547.4463552/94‑1 (MQ=60)
                             TTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGC                                                                  <  SRR4302547.5314531/94‑1 (MQ=60)
                              TTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCT                                                                 <  SRR4302547.446100/94‑1 (MQ=60)
                              TTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCT                                                                 <  SRR4302547.3625417/94‑1 (MQ=60)
                               TAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCTGGTTGGTCCAGAGCACGGACCAGTGATtgatg                                                                >  SRR4302547.4109549/1‑89 (MQ=60)
                                AATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGC                                                               >  SRR4302547.371982/1‑94 (MQ=60)
                                AATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGC                                                               >  SRR4302547.599040/1‑94 (MQ=60)
                                 ATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCT                                                              <  SRR4302547.4442671/94‑1 (MQ=60)
                                  TACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTC                                                             <  SRR4302547.4875408/94‑1 (MQ=60)
                                    CGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGA                                                           >  SRR4302547.2426028/1‑94 (MQ=60)
                                     GTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGAT                                                          <  SRR4302547.1292681/94‑1 (MQ=60)
                                      TTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATG                                                         <  SRR4302547.944072/94‑1 (MQ=60)
                                      TTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATG                                                         <  SRR4302547.2152083/94‑1 (MQ=60)
                                      TTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATG                                                         <  SRR4302547.2931419/94‑1 (MQ=60)
                                       TTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGC                                                        <  SRR4302547.5755509/94‑1 (MQ=60)
                                       TTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGC                                                        <  SRR4302547.5739337/94‑1 (MQ=60)
                                           CCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAAT                                                    >  SRR4302547.5924436/1‑94 (MQ=60)
                                            CCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATC                                                   >  SRR4302547.2293167/1‑94 (MQ=60)
                                             CGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCG                                                  <  SRR4302547.5221250/94‑1 (MQ=60)
                                             CGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCG                                                  >  SRR4302547.1554549/1‑94 (MQ=60)
                                             CGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGAACAGTGATGGCTGCTCGATGCCAATCG                                                  >  SRR4302547.6083588/1‑94 (MQ=60)
                                              GACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGC                                                 >  SRR4302547.5330799/1‑94 (MQ=60)
                                              GACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGC                                                 <  SRR4302547.4514849/94‑1 (MQ=60)
                                              GACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGC                                                 >  SRR4302547.6354444/1‑94 (MQ=60)
                                              GACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGC                                                 >  SRR4302547.2631813/1‑94 (MQ=60)
                                              GACGATCGCTGGCACAAGCTTGACGCGAAATTAACCCTTTTTCTTCCGGGTGGTTCAGAGTACGGACCAGTGAAGGCTGCTCGCTGCCAAGCGC                                                 >  SRR4302547.968337/1‑94 (MQ=60)
                                                CGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTT                                               >  SRR4302547.682848/1‑94 (MQ=60)
                                                CGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTT                                               >  SRR4302547.4442649/1‑94 (MQ=60)
                                                 GATCGCTGGCACAAGTTTGATGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTT                                              >  SRR4302547.6630933/1‑94 (MQ=60)
                                                 GATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTT                                              >  SRR4302547.924702/1‑94 (MQ=60)
                                                  ATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTT                                             <  SRR4302547.2651818/94‑1 (MQ=60)
                                                  ATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTT                                             <  SRR4302547.5282519/94‑1 (MQ=60)
                                                   TCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTG                                            >  SRR4302547.5335078/1‑94 (MQ=60)
                                                    CGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGC                                           >  SRR4302547.6402743/1‑94 (MQ=60)
                                                     GCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCC                                          >  SRR4302547.2018995/1‑94 (MQ=60)
                                                      CTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCA                                         >  SRR4302547.4633007/1‑94 (MQ=60)
                                                      CTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCA                                         <  SRR4302547.2848471/94‑1 (MQ=60)
                                                       TGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAG                                        <  SRR4302547.592884/94‑1 (MQ=60)
                                                         GCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCAAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTT                                      <  SRR4302547.4896227/94‑1 (MQ=60)
                                                          CACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTG                                     >  SRR4302547.6384988/1‑94 (MQ=60)
                                                           ACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGA                                    >  SRR4302547.2163340/1‑94 (MQ=60)
                                                           ACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGA                                    <  SRR4302547.4020257/94‑1 (MQ=60)
                                                               GTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTT                                <  SRR4302547.5932790/94‑1 (MQ=60)
                                                               GTTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTT                                >  SRR4302547.5935316/1‑94 (MQ=60)
                                                                TTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTG                               <  SRR4302547.3439224/94‑1 (MQ=60)
                                                                TTTGACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTG                               <  SRR4302547.3823389/94‑1 (MQ=60)
                                                                   GACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGA                            >  SRR4302547.2309169/1‑94 (MQ=60)
                                                                    ACGCGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGAC                           >  SRR4302547.3835488/1‑94 (MQ=60)
                                                                      GCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTG                         <  SRR4302547.5514847/94‑1 (MQ=60)
                                                                       CGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGG                        <  SRR4302547.4660431/94‑1 (MQ=60)
                                                                       CGAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGG                        <  SRR4302547.2283599/94‑1 (MQ=60)
                                                                        GAAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGT                       >  SRR4302547.2513746/1‑94 (MQ=60)
                                                                         AAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTC                      >  SRR4302547.3002663/1‑94 (MQ=60)
                                                                         AAATTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTC                      <  SRR4302547.4352775/94‑1 (MQ=60)
                                                                          AATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCT                     <  SRR4302547.5112303/94‑1 (MQ=60)
                                                                          AAATAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCT                     >  SRR4302547.3536526/1‑94 (MQ=60)
                                                                            TTAACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGG                   >  SRR4302547.3557729/1‑94 (MQ=60)
                                                                              AACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAG                 >  SRR4302547.5414755/1‑94 (MQ=60)
                                                                              AACCCTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAG                 >  SRR4302547.515661/1‑94 (MQ=60)
                                                                                  CTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAA             >  SRR4302547.465459/1‑94 (MQ=60)
                                                                                  CTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAA             <  SRR4302547.4220641/94‑1 (MQ=60)
                                                                                  CTTTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAA             >  SRR4302547.1581187/1‑94 (MQ=60)
                                                                                  CTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAA             <  SRR4302547.3002694/94‑1 (MQ=60)
                                                                                   TTTTTCTTCCGATTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAC            <  SRR4302547.1127892/94‑1 (MQ=60)
                                                                                   TTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAAC            >  SRR4302547.2766769/1‑94 (MQ=60)
                                                                                    TTTTCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGTCTGCTCGATGCCAATCGCTTTGGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACT           <  SRR4302547.1801407/94‑1 (MQ=60)
                                                                                       TCTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGGAACTGAT        >  SRR4302547.6622868/1‑94 (MQ=60)
                                                                                        CTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATG       >  SRR4302547.645314/1‑94 (MQ=60)
                                                                                        CTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATG       <  SRR4302547.4632711/94‑1 (MQ=60)
                                                                                        CTTCCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATG       >  SRR4302547.160737/1‑94 (MQ=60)
                                                                                           CCGGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGAT    >  SRR4302547.4805968/1‑94 (MQ=60)
                                                                                             GGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATAT  >  SRR4302547.6537930/1‑94 (MQ=60)
                                                                                             GGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATAT  >  SRR4302547.3419860/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGATCAGCGGCTCTGCCTTTTCCGTCAGTTTAATACGTTTAGCCCGACGATCGCTGGCACAAGTTTGACGCGAAATTAACCCTTTTTCTTCCAGTTGGTCCAGAGTACGGACCAGTGATGGCTGCTCGATGCCAATCGCTTTTGCCAGTTGAATTTGCGACTGGTCTGGAGGTAACTGATGGATAT  >  NC_000913/1718547‑1718733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑