Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,402,412 C→T A74A (GCG→GCA)  nuoB ← NADH dehydrogenase I chain B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,402,4120CT97.9% 904.2 / 2.5 243A74A (GCG→GCA) nuoBNADH dehydrogenase I chain B
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base T (121/117);  total (122/121)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

Only 100 of 255 total aligned reads displayed.

GGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAT  >  NC_000913/2402322‑2402501
                                                                                          |                                                                                         
ggTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACtgc                                                                                         <  2:1447416/93‑1 (MQ=255)
  tAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCgg                                                                                       <  2:2119551/93‑1 (MQ=255)
    aaGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTa                                                                                     <  1:754301/93‑1 (MQ=255)
    aaGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTa                                                                                     >  1:4771412/1‑93 (MQ=255)
      gCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTaaa                                                                                   <  2:1121130/93‑1 (MQ=255)
         ggTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGa                                                                                <  1:4195394/93‑1 (MQ=255)
           ttCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAg                                                                              >  1:5801829/1‑93 (MQ=255)
               tGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCAc                                                                          >  1:2310445/1‑93 (MQ=255)
                gCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCAcc                                                                         >  1:2411642/1‑93 (MQ=255)
                 cAACCGCCATCAGGACAGGCTGACGCGGCGAAGCACGCAATGCTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCa                                                                        <  1:4689356/93‑1 (MQ=255)
                 cAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCa                                                                        <  1:258189/93‑1 (MQ=255)
                 cAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCa                                                                        <  2:3400700/93‑1 (MQ=255)
                  aaCCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAt                                                                       >  2:2161765/1‑93 (MQ=255)
                   accaccATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAtc                                                                      >  2:5872923/1‑93 (MQ=255)
                   accaccATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAtc                                                                      >  2:5033354/1‑93 (MQ=255)
                    ccaccaTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAtct                                                                     >  1:2104513/1‑93 (MQ=255)
                      accaTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCa                                                                   >  1:5927213/1‑93 (MQ=255)
                        caTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAAc                                                                 <  2:5401924/93‑1 (MQ=255)
                         aTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACg                                                                >  1:3229972/1‑93 (MQ=255)
                          tCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGt                                                               <  2:4528905/93‑1 (MQ=255)
                            aGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTaa                                                             <  1:3640019/93‑1 (MQ=255)
                            aGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTaa                                                             >  2:4784441/1‑93 (MQ=255)
                            aGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTaa                                                             >  1:1996844/1‑93 (MQ=255)
                             ggTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAc                                                            <  1:1065004/93‑1 (MQ=255)
                             ggTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAc                                                            <  2:6036553/93‑1 (MQ=255)
                             ggTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAc                                                            >  2:4300086/1‑93 (MQ=255)
                              gTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAca                                                           <  2:2557985/93‑1 (MQ=255)
                              gTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAca                                                           <  1:2985365/93‑1 (MQ=255)
                               tCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAcag                                                          >  2:1417721/1‑93 (MQ=255)
                                 aGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAcagca                                                        <  2:4903603/93‑1 (MQ=255)
                                  gCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAcagcag                                                       >  1:2138665/1‑93 (MQ=255)
                                    cTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGa                                                     >  2:5807880/1‑93 (MQ=255)
                                     tGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGaa                                                    <  1:4401672/93‑1 (MQ=255)
                                      gACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGaaa                                                   <  2:2603725/93‑1 (MQ=255)
                                      gACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGaaa                                                   >  2:1874958/1‑93 (MQ=255)
                                         gcggcgAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGAc                                                >  2:4548933/1‑93 (MQ=255)
                                         gcggcgAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGAc                                                >  2:5736971/1‑93 (MQ=255)
                                         gcggcgAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGAc                                                <  1:517566/93‑1 (MQ=255)
                                            gcgAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGa                                             >  1:3992949/1‑93 (MQ=255)
                                              gAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAg                                           >  1:899111/1‑93 (MQ=255)
                                              gAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAg                                           >  1:5631033/1‑93 (MQ=255)
                                               aaGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGt                                          <  1:2348353/93‑1 (MQ=255)
                                                aGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGtt                                         >  2:2766219/1‑93 (MQ=255)
                                                  cACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTtat                                       <  2:3478415/93‑1 (MQ=255)
                                                  cACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTtat                                       <  1:194846/93‑1 (MQ=255)
                                                   aCGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTtata                                      <  1:3909275/93‑1 (MQ=255)
                                                    cGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAc                                     >  1:3166031/1‑93 (MQ=255)
                                                    cGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAc                                     <  2:1988970/93‑1 (MQ=255)
                                                    cGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAc                                     >  1:4596216/1‑93 (MQ=255)
                                                    cGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAc                                     >  2:2236556/1‑93 (MQ=255)
                                                     gCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACg                                    <  1:670831/93‑1 (MQ=255)
                                                     gCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACg                                    >  1:6052254/1‑93 (MQ=255)
                                                     gCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACg                                    >  1:3242920/1‑93 (MQ=255)
                                                     gCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACg                                    <  1:4857522/93‑1 (MQ=255)
                                                     gCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACg                                    <  2:3894923/93‑1 (MQ=255)
                                                      cAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACgg                                   <  2:622600/93‑1 (MQ=255)
                                                       aaTACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGc                                  <  1:1744604/93‑1 (MQ=255)
                                                       aaTACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGc                                  >  2:5575418/1‑93 (MQ=255)
                                                         tACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCa                                >  1:2877958/1‑93 (MQ=255)
                                                         tACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCa                                >  1:3369625/1‑93 (MQ=255)
                                                         tACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCa                                <  1:1581797/93‑1 (MQ=255)
                                                           cTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCaaa                              >  2:1984045/1‑93 (MQ=255)
                                                            ttCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAAt                             <  2:3665473/93‑1 (MQ=255)
                                                               tGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGa                          >  2:81231/1‑93 (MQ=255)
                                                               tGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGa                          >  1:1842241/1‑93 (MQ=255)
                                                                  gcCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGtt                       >  2:4727207/1‑93 (MQ=255)
                                                                  gcCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGtt                       <  1:1196458/93‑1 (MQ=255)
                                                                   ccAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGttt                      <  2:4048006/93‑1 (MQ=255)
                                                                   ccAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGttt                      >  2:2361945/1‑93 (MQ=255)
                                                                   ccAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGttt                      <  2:1243530/93‑1 (MQ=255)
                                                                     aaaaCGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGttttt                    >  2:1617630/1‑93 (MQ=255)
                                                                      aaaCGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTa                   <  1:5130248/93‑1 (MQ=255)
                                                                      aaaCGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTa                   <  1:1255102/93‑1 (MQ=255)
                                                                         cgcgCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTTAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGa                >  1:498814/1‑93 (MQ=255)
                                                                          gcgcCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAc               >  1:1677565/1‑93 (MQ=255)
                                                                          gcgcCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAc               <  2:3993991/93‑1 (MQ=255)
                                                                           cgcCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAcc              <  1:4280728/93‑1 (MQ=255)
                                                                            gcCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAccc             <  2:3609929/93‑1 (MQ=255)
                                                                            gcCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAccc             >  2:2842330/1‑93 (MQ=255)
                                                                            gcCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAccc             >  1:2489713/1‑93 (MQ=255)
                                                                             ccACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAcccc            <  1:1034006/93‑1 (MQ=255)
                                                                              cACGTCATGCACTGTGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCa           >  2:4273589/1‑93 (MQ=255)
                                                                              cACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCa           <  1:3034694/93‑1 (MQ=255)
                                                                               aCGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAg          >  1:1481786/1‑93 (MQ=255)
                                                                               aCGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAg          >  2:1822170/1‑93 (MQ=255)
                                                                               aCGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAg          >  1:4245607/1‑93 (MQ=255)
                                                                                 gtaaTGCAAGGCGGTAAACTAAGTCTCCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGtt        <  2:3044000/90‑1 (MQ=255)
                                                                                 gTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGtt        <  2:4938501/93‑1 (MQ=255)
                                                                                 gTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGtt        <  2:3043997/93‑1 (MQ=255)
                                                                                 gTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGtt        >  2:5511186/1‑93 (MQ=255)
                                                                                   cATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTaa      >  2:1660430/1‑93 (MQ=255)
                                                                                    aTGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAc     >  1:5422557/1‑93 (MQ=255)
                                                                                    aTGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAc     >  2:3233407/1‑93 (MQ=255)
                                                                                    aTGCACGGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAc     <  1:2635264/93‑1 (MQ=255)
                                                                                     tGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAcc    <  1:5089211/93‑1 (MQ=255)
                                                                                     tGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAcc    >  1:3516128/1‑93 (MQ=255)
                                                                                      gCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCa   <  2:5128310/93‑1 (MQ=255)
                                                                                      gCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCa   <  1:2775903/93‑1 (MQ=255)
                                                                                      gCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCa   <  2:1545613/93‑1 (MQ=255)
                                                                                       cACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAt  <  2:926112/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAT  >  NC_000913/2402322‑2402501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCA  >  NC_000913/2402319‑2402505
                                                                                             |                                                                                             
TTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACT                                                                                               >  SRR4302532.2999228/1‑94 (MQ=60)
TTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACT                                                                                               >  SRR4302532.2999227/1‑94 (MQ=60)
TTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACT                                                                                               <  SRR4302532.4535173/94‑1 (MQ=60)
  TGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGC                                                                                             >  SRR4302532.896487/1‑94 (MQ=60)
  TGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGC                                                                                             >  SRR4302532.6091143/1‑94 (MQ=60)
   GGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCG                                                                                            <  SRR4302532.5308494/94‑1 (MQ=60)
    GTAAAGCAGGTTCCTTCACCCCCCATCAGGTCAGCCTGACTCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGG                                                                                           <  SRR4302532.1686287/94‑1 (MQ=60)
      AAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTA                                                                                         >  SRR4302532.4935451/1‑94 (MQ=60)
      AAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGGA                                                                                         >  SRR4302532.330488/1‑94 (MQ=60)
       AAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAA                                                                                        >  SRR4302532.5048669/1‑94 (MQ=60)
       AAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAA                                                                                        <  SRR4302532.780141/94‑1 (MQ=60)
        AGCAGGTTCCTGCAACCCCCATCAGTTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAGAACGCGCCACGTCATGCACTGCGGTAAA                                                                                       <  SRR4302532.6331107/94‑1 (MQ=60)
        AGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAA                                                                                       <  SRR4302532.3111700/94‑1 (MQ=60)
         GCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAAC                                                                                      <  SRR4302532.244426/94‑1 (MQ=60)
          CAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACG                                                                                     >  SRR4302532.4271998/1‑94 (MQ=60)
          CAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACG                                                                                     <  SRR4302532.3316793/94‑1 (MQ=60)
           AGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGA                                                                                    >  SRR4302532.4395149/1‑94 (MQ=60)
            GGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAA                                                                                   <  SRR4302532.4336189/94‑1 (MQ=60)
            GGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAA                                                                                   <  SRR4302532.4336190/94‑1 (MQ=60)
             GTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAG                                                                                  >  SRR4302532.6003459/1‑94 (MQ=60)
              TTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGT                                                                                 <  SRR4302532.5989929/94‑1 (MQ=60)
                 CTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCAC                                                                              >  SRR4302532.2387323/1‑94 (MQ=60)
                 CTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCAC                                                                              >  SRR4302532.2556049/1‑94 (MQ=60)
                  TGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACC                                                                             >  SRR4302532.2491860/1‑94 (MQ=60)
                    CAACCGCCATCAGGACAGGCTGACGCGGCGAAGCACGCAATGCTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAT                                                                           <  SRR4302532.4849293/94‑1 (MQ=60)
                    CAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAT                                                                           <  SRR4302532.4849292/94‑1 (MQ=60)
                    CAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCAT                                                                           <  SRR4302532.267147/94‑1 (MQ=60)
                     AACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATC                                                                          <  SRR4302532.731784/94‑1 (MQ=60)
                      ACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCT                                                                         >  SRR4302532.2142195/1‑94 (MQ=60)
                      ACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCT                                                                         >  SRR4302532.2174748/1‑94 (MQ=60)
                      ACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCT                                                                         <  SRR4302532.3746887/94‑1 (MQ=60)
                        CACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCA                                                                       >  SRR4302532.6132425/1‑94 (MQ=60)
                        CACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCA                                                                       >  SRR4302532.4075562/1‑94 (MQ=60)
                        CACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCA                                                                       >  SRR4302532.4443760/1‑94 (MQ=60)
                         ACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAA                                                                      >  SRR4302532.2763188/1‑94 (MQ=60)
                          CCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAAC                                                                     <  SRR4302532.534173/94‑1 (MQ=60)
                           CATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACG                                                                    >  SRR4302532.3337187/1‑94 (MQ=60)
                              CAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAA                                                                 >  SRR4302532.2063356/1‑94 (MQ=60)
                              CAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAA                                                                 >  SRR4302532.1131963/1‑94 (MQ=60)
                               AGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAC                                                                >  SRR4302532.3021391/1‑94 (MQ=60)
                               AGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAC                                                                <  SRR4302532.395908/94‑1 (MQ=60)
                               AGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAC                                                                <  SRR4302532.3762158/94‑1 (MQ=60)
                               AGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAAC                                                                <  SRR4302532.3626269/94‑1 (MQ=60)
                                GGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACA                                                               >  SRR4302532.2199869/1‑94 (MQ=60)
                                GGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACA                                                               <  SRR4302532.1100832/94‑1 (MQ=60)
                                 GTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAG                                                              <  SRR4302532.5301728/94‑1 (MQ=60)
                                 GTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAG                                                              <  SRR4302532.3084069/94‑1 (MQ=60)
                                   CAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCA                                                            <  SRR4302532.2960075/94‑1 (MQ=60)
                                    AGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAG                                                           >  SRR4302532.2210087/1‑94 (MQ=60)
                                    AGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAG                                                           <  SRR4302532.1019136/94‑1 (MQ=60)
                                      CCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGA                                                         >  SRR4302532.4221438/1‑94 (MQ=60)
                                       CTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAA                                                        >  SRR4302532.6278593/1‑94 (MQ=60)
                                        TGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAA                                                       <  SRR4302532.4551523/94‑1 (MQ=60)
                                        TGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGATAAACGAAGTCACCATCTCAACGTAACAGCAGGAAA                                                       <  SRR4302532.4935035/94‑1 (MQ=60)
                                         GACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAG                                                      >  SRR4302532.5130939/1‑94 (MQ=60)
                                           CGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGAC                                                    <  SRR4302532.1873877/94‑1 (MQ=60)
                                           CGCGCCGAAGCACGCAATACTGCTGCGCCAATGCGCGCCACGTCATGCACTGCTGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGAC                                                    <  SRR4302532.1565083/94‑1 (MQ=60)
                                            GCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACC                                                   <  SRR4302532.535636/94‑1 (MQ=60)
                                             CGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCG                                                  <  SRR4302532.2601133/94‑1 (MQ=60)
                                              GGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGA                                                 >  SRR4302532.4127441/1‑94 (MQ=60)
                                              GGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGA                                                 >  SRR4302532.2902907/1‑94 (MQ=60)
                                               GCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAA                                                <  SRR4302532.1751347/94‑1 (MQ=60)
                                                CGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAG                                               >  SRR4302532.4413814/1‑94 (MQ=60)
                                                CGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAG                                               >  SRR4302532.929775/1‑94 (MQ=60)
                                                CGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAG                                               <  SRR4302532.4126939/94‑1 (MQ=60)
                                                CGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAG                                               >  SRR4302532.5826536/1‑94 (MQ=60)
                                                CGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAG                                               >  SRR4302532.334965/1‑94 (MQ=60)
                                                CGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAG                                               <  SRR4302532.2037470/94‑1 (MQ=60)
                                                 GAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGT                                              >  SRR4302532.6045380/1‑94 (MQ=60)
                                                  AAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTT                                             <  SRR4302532.5218010/94‑1 (MQ=60)
                                                  AAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTT                                             <  SRR4302532.2426689/94‑1 (MQ=60)
                                                   AGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTA                                            >  SRR4302532.5710769/1‑94 (MQ=60)
                                                    GCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTAT                                           <  SRR4302532.4982175/94‑1 (MQ=60)
                                                    GCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTAT                                           <  SRR4302532.895307/94‑1 (MQ=60)
                                                    GCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTAT                                           <  SRR4302532.3376229/94‑1 (MQ=60)
                                                     CCCGCAATACTTCTGCGCCAAAGCGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATA                                          <  SRR4302532.107833/94‑1 (MQ=60)
                                                     CACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATA                                          <  SRR4302532.201432/94‑1 (MQ=60)
                                                      ACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAC                                         >  SRR4302532.3271175/1‑94 (MQ=60)
                                                      ACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAC                                         <  SRR4302532.2380829/94‑1 (MQ=60)
                                                      ACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAC                                         <  SRR4302532.4041086/94‑1 (MQ=60)
                                                      ACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAC                                         >  SRR4302532.4752901/1‑94 (MQ=60)
                                                       CGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACG                                        >  SRR4302532.6261254/1‑94 (MQ=60)
                                                       CGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACG                                        >  SRR4302532.3350595/1‑94 (MQ=60)
                                                        GCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGG                                       <  SRR4302532.693962/94‑1 (MQ=60)
                                                        GCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGG                                       <  SRR4302532.5024645/94‑1 (MQ=60)
                                                        GCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGG                                       <  SRR4302532.2406077/94‑1 (MQ=60)
                                                         CAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGC                                      >  SRR4302532.2010137/1‑94 (MQ=60)
                                                         CAATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGC                                      >  SRR4302532.123770/1‑94 (MQ=60)
                                                          AATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCC                                     <  SRR4302532.1803286/94‑1 (MQ=60)
                                                           ATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCA                                    >  SRR4302532.3482276/1‑94 (MQ=60)
                                                           ATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCA                                    >  SRR4302532.3090647/1‑94 (MQ=60)
                                                           ATACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCA                                    >  SRR4302532.2974189/1‑94 (MQ=60)
                                                            TACTTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAA                                   <  SRR4302532.1635347/94‑1 (MQ=60)
                                                               TTCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATT                                <  SRR4302532.2439228/94‑1 (MQ=60)
                                                                TCTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTG                               >  SRR4302532.5575764/1‑94 (MQ=60)
                                                                 CTGCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGA                              >  SRR4302532.1903431/1‑94 (MQ=60)
                                                                   GCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGT                            <  SRR4302532.182345/94‑1 (MQ=60)
                                                                   GCGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGT                            <  SRR4302532.3635553/94‑1 (MQ=60)
                                                                    CGCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGGT                           >  SRR4302532.4866292/1‑94 (MQ=60)
                                                                     GCCAAAACTCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTT                          <  SRR4302532.165959/94‑1 (MQ=60)
                                                                     GCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTT                          <  SRR4302532.2586933/94‑1 (MQ=60)
                                                                     GCCAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTT                          >  SRR4302532.5243168/1‑94 (MQ=60)
                                                                     GCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTT                          <  SRR4302532.1236466/94‑1 (MQ=60)
                                                                       CAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTT                        >  SRR4302532.1111668/1‑94 (MQ=60)
                                                                       CAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTT                        <  SRR4302532.3256103/94‑1 (MQ=60)
                                                                       CAAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTT                        <  SRR4302532.2064987/94‑1 (MQ=60)
                                                                        AAAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTA                       <  SRR4302532.2711067/94‑1 (MQ=60)
                                                                         AAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAC                      <  SRR4302532.6288077/94‑1 (MQ=60)
                                                                         AAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAC                      <  SRR4302532.5306731/94‑1 (MQ=60)
                                                                         AAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAC                      <  SRR4302532.1297097/94‑1 (MQ=60)
                                                                         AAACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAC                      >  SRR4302532.4735390/1‑94 (MQ=60)
                                                                           ACGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTTAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGA                    >  SRR4302532.516256/1‑94 (MQ=60)
                                                                            CGCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAC                   >  SRR4302532.1734281/1‑94 (MQ=60)
                                                                             GCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACC                  >  SRR4302532.2727209/1‑94 (MQ=60)
                                                                             GCGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACC                  <  SRR4302532.1509626/94‑1 (MQ=60)
                                                                              CGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCC                 >  SRR4302532.2035705/1‑94 (MQ=60)
                                                                              CGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCC                 >  SRR4302532.2304730/1‑94 (MQ=60)
                                                                              CGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCC                 <  SRR4302532.4424931/94‑1 (MQ=60)
                                                                              CGCCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCC                 >  SRR4302532.2572698/1‑94 (MQ=60)
                                                                                CCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCA               <  SRR4302532.1496402/94‑1 (MQ=60)
                                                                                CCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCA               <  SRR4302532.1068914/94‑1 (MQ=60)
                                                                                CCACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCA               >  SRR4302532.5676550/1‑94 (MQ=60)
                                                                                 CACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAG              >  SRR4302532.1531729/1‑94 (MQ=60)
                                                                                 CACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAG              <  SRR4302532.3134826/94‑1 (MQ=60)
                                                                                 CACGTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAG              >  SRR4302532.4388258/1‑94 (MQ=60)
                                                                                    GTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTA           >  SRR4302532.6153432/1‑94 (MQ=60)
                                                                                    GTCATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTA           >  SRR4302532.4733836/1‑94 (MQ=60)
                                                                                      CATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAC         >  SRR4302532.5610500/1‑94 (MQ=60)
                                                                                       ATGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACC        >  SRR4302532.3633850/1‑94 (MQ=60)
                                                                                       ATGCACGGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACC        <  SRR4302532.2723561/94‑1 (MQ=60)
                                                                                        TGCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCA       <  SRR4302532.5264198/94‑1 (MQ=60)
                                                                                         GCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAT      <  SRR4302532.2869072/94‑1 (MQ=60)
                                                                                         GCACTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAT      <  SRR4302532.3458574/94‑1 (MQ=60)
                                                                                            CTGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTCACCATGTC   >  SRR4302532.3243681/1‑94 (MQ=60)
                                                                                             TGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCA  <  SRR4302532.6167430/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCA  >  NC_000913/2402319‑2402505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑