Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,655,609 G→C R449R (CGC→CGG)  hscA ← HscA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,655,6090GC97.3% 665.6 / 5.7 188R449R (CGC→CGG) hscAHscA
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base C (77/106);  total (79/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

Only 100 of 193 total aligned reads displayed.

ACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGC  >  NC_000913/2655519‑2655698
                                                                                          |                                                                                         
aCCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGAAGCGCAAAACGCGCCAGTGACCg                                                                                         >  1:2848494/1‑93 (MQ=255)
   gTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCa                                                                                      <  2:1823447/93‑1 (MQ=255)
      ggCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTc                                                                                   >  2:406734/1‑93 (MQ=255)
       gCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTcc                                                                                  <  1:479233/93‑1 (MQ=255)
        cATCGACGTGGAACGTCACGCGAATCGGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCt                                                                                 <  1:860931/93‑1 (MQ=255)
         aTCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTg                                                                                >  2:4568496/1‑93 (MQ=255)
          tCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGc                                                                               >  2:497313/1‑93 (MQ=255)
             aCCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCAcc                                                                            <  2:5433423/93‑1 (MQ=255)
              ccTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCa                                                                           >  1:6075783/1‑93 (MQ=255)
              ccTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCa                                                                           >  1:1963571/1‑93 (MQ=255)
               cTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAg                                                                          >  1:971484/1‑93 (MQ=255)
               cTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAg                                                                          >  2:5820083/1‑93 (MQ=255)
                tGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGt                                                                         >  2:2038142/1‑93 (MQ=255)
                tGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGt                                                                         >  2:1739006/1‑93 (MQ=255)
                   accGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTcg                                                                      <  2:1057842/91‑1 (MQ=255)
                    aCGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTcgc                                                                     <  2:3630365/93‑1 (MQ=255)
                    aCGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTcgc                                                                     >  2:5405139/1‑93 (MQ=255)
                      gTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCgcgc                                                                   >  2:161420/1‑93 (MQ=255)
                      gTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCgcgc                                                                   >  2:4149393/1‑93 (MQ=255)
                       tCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCt                                                                  <  1:1028858/93‑1 (MQ=255)
                        cACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTc                                                                 >  2:3172517/1‑93 (MQ=255)
                           gcgAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCAcc                                                              >  2:4332124/1‑93 (MQ=255)
                            cgAATATGCGCCCCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCAccc                                                             <  1:2130298/93‑1 (MQ=255)
                            cgAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCAccc                                                             <  2:1499182/93‑1 (MQ=255)
                            cgAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCAccc                                                             <  2:4696898/93‑1 (MQ=255)
                             gAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTAGCGCTCACCCt                                                            >  2:3948719/1‑93 (MQ=255)
                              aaTATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTg                                                           <  2:1805173/93‑1 (MQ=255)
                                tatGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCa                                                         <  1:312680/93‑1 (MQ=255)
                                 atGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCAt                                                        <  1:3413869/93‑1 (MQ=255)
                                   gcgcACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTa                                                      >  2:4112149/1‑93 (MQ=255)
                                    cgcACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTAc                                                     <  1:5714731/93‑1 (MQ=255)
                                     gcACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACa                                                    >  1:4249214/1‑93 (MQ=255)
                                     gcACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACa                                                    >  2:4458830/1‑93 (MQ=255)
                                     gcACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACa                                                    <  2:515096/93‑1 (MQ=255)
                                      cACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACAt                                                   <  2:14177/93‑1 (MQ=255)
                                      cACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACAt                                                   <  1:3367011/93‑1 (MQ=255)
                                         cGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGa                                                >  2:4460474/1‑93 (MQ=255)
                                          gCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGAt                                               >  1:5528612/1‑93 (MQ=255)
                                           ccAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATa                                              <  1:729667/93‑1 (MQ=255)
                                           ccAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATa                                              <  1:1551570/93‑1 (MQ=255)
                                           caaGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATa                                              <  2:4846094/91‑1 (MQ=255)
                                              gCCGGTAGCGCCGGAATACCAAGCAGCGCAAAACGCGCCAGTGACAGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGAc                                           >  1:4219164/1‑93 (MQ=255)
                                               ccGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACa                                          <  2:2341855/93‑1 (MQ=255)
                                                cGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACAt                                         >  1:5895393/1‑93 (MQ=255)
                                                 ggTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATc                                        >  1:472778/1‑93 (MQ=255)
                                                  gTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCg                                       >  1:3371205/1‑93 (MQ=255)
                                                  gTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCg                                       >  1:4294898/1‑93 (MQ=255)
                                                    aGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGcc                                     <  1:3991183/93‑1 (MQ=255)
                                                    aGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGcc                                     <  1:2671711/93‑1 (MQ=255)
                                                       gcCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTc                                  <  1:799305/93‑1 (MQ=255)
                                                       gcCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTc                                  <  2:729552/93‑1 (MQ=255)
                                                       gcCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTc                                  >  1:1600297/1‑93 (MQ=255)
                                                         cGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTg                                <  1:3581741/93‑1 (MQ=255)
                                                          ggAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGa                               >  1:3938455/1‑93 (MQ=255)
                                                          ggAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGa                               <  2:3735017/93‑1 (MQ=255)
                                                           gAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGAc                              >  1:1318071/1‑93 (MQ=255)
                                                           gAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGAc                              >  1:2904936/1‑93 (MQ=255)
                                                             aTACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCa                            <  1:3188130/93‑1 (MQ=255)
                                                              tACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCAt                           >  1:897097/1‑93 (MQ=255)
                                                              tACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCAt                           >  1:828411/1‑93 (MQ=255)
                                                              tACCACGCAGCGCAAAACGCGCCAGTGACCGGAAGTCCTGCACCAGTTCGCGGTCACACTGCATTACATGGATAGACATCGCCGTCTGACCAt                           >  2:261804/1‑93 (MQ=255)
                                                                ccACGCAGCGCAAAACGCGCCAGTGACCGGCCGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCt                         >  2:4224704/1‑93 (MQ=255)
                                                                ccACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCt                         <  2:392345/93‑1 (MQ=255)
                                                                 cACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCtt                        <  2:3876798/93‑1 (MQ=255)
                                                                  aCGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCttt                       >  1:3796126/1‑93 (MQ=255)
                                                                  aCGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCttt                       <  2:4187013/93‑1 (MQ=255)
                                                                      aGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAg                   <  2:1878621/93‑1 (MQ=255)
                                                                       gcgcAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAgg                  >  1:2065091/1‑93 (MQ=255)
                                                                       gcgcAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAgg                  >  1:5006954/1‑93 (MQ=255)
                                                                        cgcAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAggt                 <  1:3232163/93‑1 (MQ=255)
                                                                        cgcAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCACTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAggt                 <  2:2285726/93‑1 (MQ=255)
                                                                          cAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAggtgg               <  1:4652155/93‑1 (MQ=255)
                                                                           aaaaCGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAggtggt              <  2:2789446/93‑1 (MQ=255)
                                                                            aaaCGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGgtggtg             <  2:441122/93‑1 (MQ=255)
                                                                            aaaCGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGgtggtg             <  2:2211171/93‑1 (MQ=255)
                                                                             aaCGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGa            >  2:5935039/1‑93 (MQ=255)
                                                                              aCGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGAGaa           <  1:4141141/93‑1 (MQ=255)
                                                                               cgcgCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGaaa          <  2:2179650/93‑1 (MQ=255)
                                                                               cgcgCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGaaa          <  2:4520914/93‑1 (MQ=255)
                                                                               cgcgCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGaaa          <  2:1781826/93‑1 (MQ=255)
                                                                               cgcgCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGaaa          <  1:880044/93‑1 (MQ=255)
                                                                                 cgcCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATc        <  2:5155195/93‑1 (MQ=255)
                                                                                 cgcCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATc        <  1:1155646/93‑1 (MQ=255)
                                                                                 cgcCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATc        <  2:2366170/93‑1 (MQ=255)
                                                                                 cgcCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATc        <  1:3782382/93‑1 (MQ=255)
                                                                                 caccAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATc        <  1:795109/91‑1 (MQ=255)
                                                                                  gcCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATcc       <  2:2591367/93‑1 (MQ=255)
                                                                                  gcCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATcc       >  1:409742/1‑93 (MQ=255)
                                                                                  gcCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATcc       <  2:3985073/93‑1 (MQ=255)
                                                                                   ccAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCt      <  1:2288260/93‑1 (MQ=255)
                                                                                    cAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTg     <  1:725371/93‑1 (MQ=255)
                                                                                    cAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTg     <  1:2712872/93‑1 (MQ=255)
                                                                                    cAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTg     >  2:5248432/1‑93 (MQ=255)
                                                                                    cAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTg     >  1:4715968/1‑93 (MQ=255)
                                                                                     aGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGa    <  2:1130716/93‑1 (MQ=255)
                                                                                     aGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGa    >  2:1398221/1‑93 (MQ=255)
                                                                                     aGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGa    <  2:1966081/93‑1 (MQ=255)
                                                                                       tGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAgc  <  1:3206745/93‑1 (MQ=255)
                                                                                       tGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAgc  <  2:2893586/93‑1 (MQ=255)
                                                                                       tGACCGGCAGCCCTGCACCAGTTTGCGCTAACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAgc  >  1:4317150/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGC  >  NC_000913/2655519‑2655698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGG  >  NC_000913/2655517‑2655702
                                                                                            |                                                                                             
agcccggcgcCATCGACCGGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACC                                                                                              <  SRR4302532.2055480/84‑1 (MQ=60)
AGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACC                                                                                              <  SRR4302532.5139027/94‑1 (MQ=60)
AGACCGTCGCCATCGACCTGAAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACC                                                                                              <  SRR4302532.3510518/94‑1 (MQ=60)
 GACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCG                                                                                             <  SRR4302532.2949688/94‑1 (MQ=60)
 GACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGAAGCGCAAAACGCGCCAGTGACCG                                                                                             >  SRR4302532.2943721/1‑94 (MQ=60)
         GCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCT                                                                                     <  SRR4302532.3275394/94‑1 (MQ=60)
         GCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCT                                                                                     <  SRR4302532.495939/94‑1 (MQ=60)
          CATCGACGTGGAACGTCACGCGAATCGGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTG                                                                                    <  SRR4302532.890491/94‑1 (MQ=60)
          CATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCACAACGCGCCAGTGACCCGCAGTCCTG                                                                                    >  SRR4302532.866797/1‑94 (MQ=60)
               ACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCA                                                                               >  SRR4302532.6285599/1‑94 (MQ=60)
               ACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCA                                                                               >  SRR4302532.2028787/1‑94 (MQ=60)
                CCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAG                                                                              >  SRR4302532.1004630/1‑94 (MQ=60)
                CCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAG                                                                              <  SRR4302532.799002/94‑1 (MQ=60)
                CCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAG                                                                              <  SRR4302532.854387/94‑1 (MQ=60)
                 CTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGT                                                                             >  SRR4302532.3359270/1‑94 (MQ=60)
                 CTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGT                                                                             >  SRR4302532.2878987/1‑94 (MQ=60)
                  TGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTT                                                                            >  SRR4302532.1698348/1‑94 (MQ=60)
                     AACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACAAAGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGC                                                                         >  SRR4302532.4461584/1‑94 (MQ=60)
                         TCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTC                                                                     >  SRR4302532.980154/1‑94 (MQ=60)
                         TCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTC                                                                     <  SRR4302532.1063641/94‑1 (MQ=60)
                           ACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCAC                                                                   >  SRR4302532.2308349/1‑94 (MQ=60)
                            CGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGCTCGCGCTCcac                                                                  >  SRR4302532.923885/1‑91 (MQ=60)
                              CGAATATGCGCCCCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCT                                                                <  SRR4302532.2201456/94‑1 (MQ=60)
                                 ATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCCGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCA                                                             >  SRR4302532.3336120/1‑94 (MQ=60)
                                  TATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCAT                                                            <  SRR4302532.323524/94‑1 (MQ=60)
                                   ATGCGCCCCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATT                                                           <  SRR4302532.2589818/94‑1 (MQ=60)
                                   ATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATT                                                           <  SRR4302532.3528212/94‑1 (MQ=60)
                                   AGGCGCACTGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATT                                                           >  SRR4302532.3984924/1‑94 (MQ=60)
                                      CGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACA                                                        <  SRR4302532.5913856/94‑1 (MQ=60)
                                      CGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACA                                                        >  SRR4302532.1472418/1‑94 (MQ=60)
                                      CGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACA                                                        >  SRR4302532.4391993/1‑94 (MQ=60)
                                        CACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATG                                                      <  SRR4302532.3479554/94‑1 (MQ=60)
                                          CCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGA                                                    >  SRR4302532.2776686/1‑94 (MQ=60)
                                          CCGCCAGCCGGTAGCCCCGGAATACCACGCAGCGCATAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACACTGCATTACATGGT                                                    >  SRR4302532.5269336/1‑94 (MQ=60)
                                           CGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGAT                                                   >  SRR4302532.5720471/1‑94 (MQ=60)
                                             CCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAG                                                 >  SRR4302532.4296511/1‑94 (MQ=60)
                                             CCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAG                                                 <  SRR4302532.1604022/94‑1 (MQ=60)
                                             CCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAG                                                 <  SRR4302532.754535/94‑1 (MQ=60)
                                              CAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGA                                                <  SRR4302532.455000/94‑1 (MQ=60)
                                               AGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGAC                                               >  SRR4302532.753003/1‑94 (MQ=60)
                                               AGCCGGTAGCGCCGGAATACCAAGCAGCGCAAAACGCGCCAGTGACAGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGAC                                               >  SRR4302532.4360779/1‑94 (MQ=60)
                                                 CCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACAT                                             >  SRR4302532.6099555/1‑94 (MQ=60)
                                                  CGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATC                                            >  SRR4302532.489251/1‑94 (MQ=60)
                                                  CGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATC                                            >  SRR4302532.6148822/1‑94 (MQ=60)
                                                  CGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATC                                            >  SRR4302532.489250/1‑94 (MQ=60)
                                                   GGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCG                                           >  SRR4302532.3483924/1‑94 (MQ=60)
                                                   GGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCG                                           >  SRR4302532.3483925/1‑94 (MQ=60)
                                                   GGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCG                                           >  SRR4302532.4439941/1‑94 (MQ=60)
                                                   GGTAGCGCCGGAATACCACGCAGCACAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCG                                           >  SRR4302532.3819071/1‑94 (MQ=60)
                                                     aaggactGGAATGCCACGCAGCGCAAAGCGCTCCAGTGACCGGCAGTCATGCCCCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCC                                         <  SRR4302532.5898653/87‑1 (MQ=60)
                                                      AGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCG                                        <  SRR4302532.4125626/94‑1 (MQ=60)
                                                      AGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCG                                        <  SRR4302532.2761380/94‑1 (MQ=60)
                                                      AGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCG                                        <  SRR4302532.3509252/94‑1 (MQ=60)
                                                        CGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATGGCCGTC                                      >  SRR4302532.1994473/1‑94 (MQ=60)
                                                        CGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTC                                      >  SRR4302532.1654464/1‑94 (MQ=60)
                                                         GCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCT                                     <  SRR4302532.826629/94‑1 (MQ=60)
                                                         CCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCT                                     <  SRR4302532.2181928/94‑1 (MQ=60)
                                                         CCCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCT                                     <  SRR4302532.1273035/94‑1 (MQ=60)
                                                          CCGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTG                                    <  SRR4302532.905241/94‑1 (MQ=60)
                                                           CGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGA                                   >  SRR4302532.4071168/1‑94 (MQ=60)
                                                           CGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGA                                   >  SRR4302532.4916080/1‑94 (MQ=60)
                                                           CGGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGA                                   <  SRR4302532.3701564/94‑1 (MQ=60)
                                                            GGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGAC                                  >  SRR4302532.3001845/1‑94 (MQ=60)
                                                            GGAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGAC                                  >  SRR4302532.1362130/1‑94 (MQ=60)
                                                             GAATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACC                                 >  SRR4302532.4362939/1‑94 (MQ=60)
                                                              AATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTGCTGCCCCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCTTCTGACCA                                <  SRR4302532.3380818/94‑1 (MQ=60)
                                                               ATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCAT                               >  SRR4302532.927698/1‑94 (MQ=60)
                                                               ATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCAT                               >  SRR4302532.856752/1‑94 (MQ=60)
                                                               ATACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCAT                               <  SRR4302532.3293981/94‑1 (MQ=60)
                                                                 ACCACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCT                             <  SRR4302532.4820710/94‑1 (MQ=60)
                                                                 ACCACGAGGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACAGGGATAGCCATCGCCGTCTGACCATCT                             <  SRR4302532.3555934/94‑1 (MQ=60)
                                                                   CACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTT                           >  SRR4302532.3924237/1‑94 (MQ=60)
                                                                    ACGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTA                          <  SRR4302532.2966228/94‑1 (MQ=60)
                                                                     CGCAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAA                         <  SRR4302532.2050229/94‑1 (MQ=60)
                                                                       CAGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAG                       >  SRR4302532.5940052/1‑94 (MQ=60)
                                                                        AGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGG                      >  SRR4302532.2133893/1‑94 (MQ=60)
                                                                        AGCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGG                      >  SRR4302532.5178559/1‑94 (MQ=60)
                                                                         GCGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGT                     <  SRR4302532.5902958/94‑1 (MQ=60)
                                                                          CGCAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTG                    <  SRR4302532.3339436/94‑1 (MQ=60)
                                                                          CCCAAAACGCGCCAGGGACCGGCAGTCCTGCACCAGTTCGCCCTCACCCTCCAATACATGTATAGACATCGCCGTCTGACCATCTTTAAAGGTG                    >  SRR4302532.2122730/1‑94 (MQ=60)
                                                                            CAAAACGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGT                  <  SRR4302532.4810764/94‑1 (MQ=60)
                                                                                ACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGAGAAA              <  SRR4302532.4280064/94‑1 (MQ=60)
                                                                                 CGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAAT             <  SRR4302532.4130722/94‑1 (MQ=60)
                                                                                 CGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAAT             <  SRR4302532.910138/94‑1 (MQ=60)
                                                                                 CGCGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAAT             <  SRR4302532.425969/94‑1 (MQ=60)
                                                                                   CGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCC           <  SRR4302532.3909881/94‑1 (MQ=60)
                                                                                   CGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCC           <  SRR4302532.1194203/94‑1 (MQ=60)
                                                                                   CGCCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCC           >  SRR4302532.424026/1‑94 (MQ=60)
                                                                                   CACCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCC           <  SRR4302532.822303/94‑1 (MQ=60)
                                                                                     CCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTG         <  SRR4302532.2364204/94‑1 (MQ=60)
                                                                                     CCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTG         >  SRR4302532.4876878/1‑94 (MQ=60)
                                                                                     CCAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTG         <  SRR4302532.3911790/94‑1 (MQ=60)
                                                                                      CAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGA        <  SRR4302532.5022104/94‑1 (MQ=60)
                                                                                      CAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGA        <  SRR4302532.750093/94‑1 (MQ=60)
                                                                                      CAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGA        >  SRR4302532.668199/1‑94 (MQ=60)
                                                                                      CAGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGA        <  SRR4302532.2804117/94‑1 (MQ=60)
                                                                                       AGTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAG       <  SRR4302532.1632440/94‑1 (MQ=60)
                                                                                        GTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGC      <  SRR4302532.3072573/94‑1 (MQ=60)
                                                                                        GTGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGC      <  SRR4302532.4089968/94‑1 (MQ=60)
                                                                                        GTGACCGGCAGCCCTGCACCAGTTTGCGCTAACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGC      >  SRR4302532.4463086/1‑94 (MQ=60)
                                                                                         TGACCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCG     <  SRR4302532.3313216/94‑1 (MQ=60)
                                                                                            CCGGCAGTCCTGCATCAGTTCGCGCTCCCCCTGCATTACGTGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGG  <  SRR4302532.2313473/94‑1 (MQ=60)
                                                                                            CCGGCAGTCCTGCACGAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGG  <  SRR4302532.870361/94‑1 (MQ=60)
                                                                                            |                                                                                             
AGACCGTCGGCATCGACCTGGAACGTCACGCGAATATGCGCACCGCCAGCCGGTAGCGCCGGAATACCACGCAGCGCAAAACGCGCCAGTGAGCGGCAGTCCTGCACCAGTTCGCGCTCACCCTGCATTACATGGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGG  >  NC_000913/2655517‑2655702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑