Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,691,024 A→T F847Y (TTT→TAT)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,0240AT100.0% 799.6 / NA 211F847Y (TTT→TAT) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (90/121);  total (90/121)

Only 100 of 220 total aligned reads displayed.

CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGC  >  NC_000913/2690934‑2691113
                                                                                          |                                                                                         
cGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataa                                                                                         <  2:5911162/93‑1 (MQ=255)
 gCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                                        <  1:5707930/93‑1 (MQ=255)
 gCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGataaa                                                                                        >  1:5125691/1‑93 (MQ=255)
   tgtgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAcc                                                                                      >  1:1167252/1‑93 (MQ=255)
    gtgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCt                                                                                     <  2:5753094/93‑1 (MQ=255)
     tgCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAACTg                                                                                    >  1:1832827/1‑93 (MQ=255)
      gCAACCAGCCCCTGAAGCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATATGCCGGTTTGTCGCCAAGCTGACGATAAACCTgc                                                                                   <  1:4684827/93‑1 (MQ=255)
         aCCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTCGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGcc                                                                                >  1:4754735/1‑93 (MQ=255)
          ccAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCa                                                                               <  1:4477076/93‑1 (MQ=255)
          ccAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCa                                                                               >  2:2210481/1‑93 (MQ=255)
          ccAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTGGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCa                                                                               <  2:3683546/93‑1 (MQ=255)
           cAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAg                                                                              <  2:4660890/93‑1 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                                             >  2:4032518/1‑93 (MQ=255)
            aGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgc                                                                             >  1:897454/1‑93 (MQ=255)
             gcgcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAgcc                                                                            >  2:1984833/1‑92 (MQ=255)
               gcCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGcc                                                                          >  1:5511483/1‑93 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  1:1027863/93‑1 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        >  2:3914695/1‑93 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  2:3973840/93‑1 (MQ=255)
                 cTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCATACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGt                                                                        <  1:4287014/93‑1 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtt                                                                       <  1:1966201/93‑1 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtt                                                                       >  2:5438977/1‑93 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGtt                                                                       >  1:3204048/1‑93 (MQ=255)
                  tGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAACTGACGATAAACCTGCGCCAGCGCCGtt                                                                       >  1:3015753/1‑93 (MQ=255)
                   gAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTg                                                                      >  1:332308/1‑93 (MQ=255)
                    aaTCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTGCATCTGCCGGTTTGTCGCAAAGCTGACGATAAACCTGCGCCAGCGCCGTTgc                                                                     <  1:4383220/93‑1 (MQ=255)
                      tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcgc                                                                   <  2:4509890/93‑1 (MQ=255)
                      tCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTgcgc                                                                   >  1:1004538/1‑93 (MQ=255)
                           tCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                                                              >  2:908932/1‑93 (MQ=255)
                           gcATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgc                                                              <  2:528611/92‑1 (MQ=255)
                            cATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAACCCTGCGCCAGCGCCGTTGCGCCCAgcg                                                             <  2:3157829/93‑1 (MQ=255)
                            cATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcg                                                             <  1:2727686/93‑1 (MQ=255)
                             aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCTAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                                            >  2:3771028/1‑93 (MQ=255)
                             aTAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAgcgc                                                            >  1:3133009/1‑93 (MQ=255)
                                gAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGtt                                                         >  2:6055939/1‑93 (MQ=255)
                                 aaGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTa                                                        >  2:5879932/1‑93 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  1:3764182/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  2:5143728/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       >  2:4890444/1‑93 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       <  1:123668/93‑1 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAt                                                       >  2:4171662/1‑93 (MQ=255)
                                  aGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGCTAt                                                       >  1:3717080/1‑93 (MQ=255)
                                     cTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGc                                                    <  2:6089413/93‑1 (MQ=255)
                                      tttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                                   >  1:1885736/1‑93 (MQ=255)
                                      tttCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGcc                                                   >  2:4716755/1‑93 (MQ=255)
                                         cAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCttt                                                <  1:689942/93‑1 (MQ=255)
                                          aGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTg                                               <  2:3765027/93‑1 (MQ=255)
                                           gTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGc                                              <  1:855438/93‑1 (MQ=255)
                                            ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                                             <  2:167807/93‑1 (MQ=255)
                                            ttGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGcc                                             <  2:1448634/93‑1 (MQ=255)
                                                 cgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat                                        <  2:2882658/93‑1 (MQ=255)
                                                 cgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat                                        <  1:5428935/93‑1 (MQ=255)
                                                 cgACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaat                                        <  1:485785/93‑1 (MQ=255)
                                                  gACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatc                                       <  2:3206048/93‑1 (MQ=255)
                                                  gACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatc                                       >  2:127056/1‑93 (MQ=255)
                                                   aCATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatca                                      >  1:2831716/1‑93 (MQ=255)
                                                     aTCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat                                    >  2:5585487/1‑93 (MQ=255)
                                                     aTCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat                                    <  1:2393208/93‑1 (MQ=255)
                                                     aTCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat                                    >  2:161458/1‑93 (MQ=255)
                                                     aTCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat                                    <  2:1064480/93‑1 (MQ=255)
                                                     aTCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAaatcaat                                    >  1:5902244/1‑93 (MQ=255)
                                                       cgcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAatcaatca                                  <  1:1534208/93‑1 (MQ=255)
                                                       cgcgTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAatcaatca                                  <  2:4311251/93‑1 (MQ=255)
                                                          gTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagca                               >  1:3883899/1‑93 (MQ=255)
                                                          gTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagca                               <  2:299557/93‑1 (MQ=255)
                                                           tACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATcagcag                              >  1:1210491/1‑93 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          >  1:5765975/1‑93 (MQ=255)
                                                               tCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGt                          >  2:3548446/1‑93 (MQ=255)
                                                                 tGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGc                        <  1:2105688/93‑1 (MQ=255)
                                                                 tGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGc                        <  1:511581/93‑1 (MQ=255)
                                                                 tGCCGGTTTGTCACCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGc                        <  2:1976402/93‑1 (MQ=255)
                                                                  gCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCg                       >  2:671966/1‑93 (MQ=255)
                                                                   ccGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGt                      >  1:5794737/1‑93 (MQ=255)
                                                                    cGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGtt                     >  2:4242026/1‑93 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCATCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    <  1:5150807/93‑1 (MQ=255)
                                                                     ggTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTa                    <  2:4402219/93‑1 (MQ=255)
                                                                      gTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAt                   >  1:2153727/1‑93 (MQ=255)
                                                                      gTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAt                   >  2:2627732/1‑93 (MQ=255)
                                                                       tttGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATc                  >  1:4506379/1‑93 (MQ=255)
                                                                        ttGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCGCAAATCAAGCAGCAGCAGTGCGTTATCt                 <  1:4736103/93‑1 (MQ=255)
                                                                        ttGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCt                 <  1:433265/93‑1 (MQ=255)
                                                                         tGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCtt                <  1:645108/93‑1 (MQ=255)
                                                                           tCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCGGTGCGTTATCTTCg              <  1:3109034/93‑1 (MQ=255)
                                                                           tCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCg              <  1:4271787/93‑1 (MQ=255)
                                                                           tCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCg              >  2:891163/1‑93 (MQ=255)
                                                                           tCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCg              >  1:2316451/1‑93 (MQ=255)
                                                                              ccAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTa           >  1:1183622/1‑93 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          <  2:3347781/93‑1 (MQ=255)
                                                                               cAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAg          <  2:5353738/93‑1 (MQ=255)
                                                                                aaGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGa         <  1:2824509/93‑1 (MQ=255)
                                                                                 aGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGaa        >  1:3842438/1‑93 (MQ=255)
                                                                                  gCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGaaa       >  1:257912/1‑93 (MQ=255)
                                                                                  gCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGaaa       <  2:1777681/93‑1 (MQ=255)
                                                                                    tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGc     <  1:1326513/93‑1 (MQ=255)
                                                                                    tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGc     >  2:2525280/1‑93 (MQ=255)
                                                                                    tGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGc     <  2:5701863/93‑1 (MQ=255)
                                                                                      aCGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTg   <  2:2750994/93‑1 (MQ=255)
                                                                                      aCGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTg   >  1:2803178/1‑93 (MQ=255)
                                                                                      aCGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTg   >  1:5370186/1‑93 (MQ=255)
                                                                                       cGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTgc  <  1:3990903/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGC  >  NC_000913/2690934‑2691113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCG  >  NC_000913/2690934‑2691117
                                                                                          |                                                                                             
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                            >  SRR4302532.5302007/1‑94 (MQ=60)
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                            >  SRR4302532.2000559/1‑94 (MQ=60)
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAA                                                                                            >  SRR4302532.5858914/1‑94 (MQ=60)
 GCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAC                                                                                           <  SRR4302532.5906919/94‑1 (MQ=60)
  CTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACC                                                                                          >  SRR4302532.1206240/1‑94 (MQ=60)
   TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                         <  SRR4302532.2072433/94‑1 (MQ=60)
   TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                         >  SRR4302532.619909/1‑94 (MQ=60)
   TGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCT                                                                                         <  SRR4302532.1268398/94‑1 (MQ=60)
    GTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAAACTG                                                                                        >  SRR4302532.1893787/1‑94 (MQ=60)
      GCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCG                                                                                      >  SRR4302532.5620505/1‑94 (MQ=60)
      GCAACCAGCCCCTGAAGCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATATGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCG                                                                                      <  SRR4302532.4844597/94‑1 (MQ=60)
        AACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCC                                                                                    >  SRR4302532.2018515/1‑94 (MQ=60)
        AACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCC                                                                                    >  SRR4302532.4917348/1‑94 (MQ=60)
        AACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTCGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCC                                                                                    >  SRR4302532.4917349/1‑94 (MQ=60)
          ctagctcctaaaaCGCGTCATAGAGGCCGTTCAGTTGCGAGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAACCCTGCGCCAG                                                                                  <  SRR4302532.4629805/81‑1 (MQ=60)
          CCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAG                                                                                  <  SRR4302532.4629807/94‑1 (MQ=60)
          CCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAG                                                                                  <  SRR4302532.4629806/94‑1 (MQ=60)
           CAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGC                                                                                 >  SRR4302532.928074/1‑94 (MQ=60)
              CGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCC                                                                              >  SRR4302532.5702735/1‑94 (MQ=60)
               GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                             >  SRR4302532.1538245/1‑94 (MQ=60)
               GCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCG                                                                             >  SRR4302532.5931624/1‑94 (MQ=60)
                CCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGT                                                                            >  SRR4302532.4870565/1‑94 (MQ=60)
                 CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           <  SRR4302532.1062617/94‑1 (MQ=60)
                 CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           >  SRR4302532.3310431/1‑94 (MQ=60)
                 CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           >  SRR4302532.2190385/1‑94 (MQ=60)
                 CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAACTGACGATAAACCTGCGCCAGCGCCGTT                                                                           >  SRR4302532.3115276/1‑94 (MQ=60)
                 CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCATACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           <  SRR4302532.4431591/94‑1 (MQ=60)
                 CTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCATACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTT                                                                           <  SRR4302532.4431592/94‑1 (MQ=60)
                  TGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTG                                                                          <  SRR4302532.2031512/94‑1 (MQ=60)
                  TGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTG                                                                          >  SRR4302532.343833/1‑94 (MQ=60)
                    AATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTGCATCTGCCGGTTTGTCGCAAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCG                                                                        <  SRR4302532.4532132/94‑1 (MQ=60)
                    AATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCG                                                                        >  SRR4302532.2657129/1‑94 (MQ=60)
                     ATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGC                                                                       >  SRR4302532.1038722/1‑94 (MQ=60)
                     ATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGC                                                                       >  SRR4302532.4446519/1‑94 (MQ=60)
                       CGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCC                                                                     >  SRR4302532.3072888/1‑94 (MQ=60)
                         CGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGAGCCAGCGCCGTTGCGCCCAG                                                                   >  SRR4302532.4767226/1‑94 (MQ=60)
                            CATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGC                                                                >  SRR4302532.3237005/1‑94 (MQ=60)
                            CATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGC                                                                <  SRR4302532.2819398/94‑1 (MQ=60)
                             ATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCG                                                               <  SRR4302532.698754/94‑1 (MQ=60)
                              TAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGT                                                              >  SRR4302532.2890982/1‑94 (MQ=60)
                               AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                             <  SRR4302532.431633/94‑1 (MQ=60)
                               AGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTT                                                             <  SRR4302532.3120188/94‑1 (MQ=60)
                                 AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           >  SRR4302532.5578457/1‑94 (MQ=60)
                                 AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTAT                                                           <  SRR4302532.2738845/94‑1 (MQ=60)
                                 AAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGCTAT                                                           >  SRR4302532.3841839/1‑94 (MQ=60)
                                  AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTCTT                                                          >  SRR4302532.2917368/1‑94 (MQ=60)
                                  AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          <  SRR4302532.1127142/94‑1 (MQ=60)
                                  AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          >  SRR4302532.4245517/1‑94 (MQ=60)
                                  AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          <  SRR4302532.127870/94‑1 (MQ=60)
                                  AGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATT                                                          <  SRR4302532.3890846/94‑1 (MQ=60)
                                   GCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTG                                                         <  SRR4302532.3510442/94‑1 (MQ=60)
                                    CCTTTCAGTTGCGCGACATCGCGTACATCTGGCGGTTTGTCTCCAAGCTTAAGATAAACCTGCGCCAGCCCCGCTGCGCCCAGCGggtgatttc                                                        >  SRR4302532.2719496/1‑85 (MQ=60)
                                    CCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGC                                                        >  SRR4302532.987523/1‑94 (MQ=60)
                                     CTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCC                                                       >  SRR4302532.1948263/1‑94 (MQ=60)
                                       TTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTT                                                     >  SRR4302532.901113/1‑94 (MQ=60)
                                         CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302532.713620/94‑1 (MQ=60)
                                         CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302532.1261560/94‑1 (MQ=60)
                                         CAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTG                                                   <  SRR4302532.3350124/94‑1 (MQ=60)
                                           GTTGCGCGACATCGCGTACGTCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCC                                                 <  SRR4302532.884832/94‑1 (MQ=60)
                                           GTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCC                                                 <  SRR4302532.884833/94‑1 (MQ=60)
                                            TTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCC                                                >  SRR4302532.4014957/1‑94 (MQ=60)
                                                 CGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATC                                           <  SRR4302532.502747/94‑1 (MQ=60)
                                                 CGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATC                                           <  SRR4302532.5617089/94‑1 (MQ=60)
                                                  GACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCA                                          >  SRR4302532.2926409/1‑94 (MQ=60)
                                                    CATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAAT                                        >  SRR4302532.6106627/1‑94 (MQ=60)
                                                     ATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATC                                       <  SRR4302532.2472922/94‑1 (MQ=60)
                                                      TCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCA                                      <  SRR4302532.1651191/94‑1 (MQ=60)
                                                       CGCGTCCATCTGCCGGTTTGTCCCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                     <  SRR4302532.5983184/94‑1 (MQ=60)
                                                       CGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAG                                     <  SRR4302532.1586047/94‑1 (MQ=60)
                                                         CGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCA                                   <  SRR4302532.4927155/94‑1 (MQ=60)
                                                         CGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCA                                   >  SRR4302532.4014974/1‑94 (MQ=60)
                                                         CGTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCA                                   <  SRR4302532.555566/94‑1 (MQ=60)
                                                          GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                  >  SRR4302532.1250990/1‑94 (MQ=60)
                                                          GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                  >  SRR4302532.6118601/1‑94 (MQ=60)
                                                          GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                  <  SRR4302532.5137349/94‑1 (MQ=60)
                                                          GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                  <  SRR4302532.3213838/94‑1 (MQ=60)
                                                          GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAG                                  <  SRR4302532.1233870/94‑1 (MQ=60)
                                                          GTACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCCCCCAGCGCGTTATTGCTGTTGCCCAAATCAATAAGCAG                                  <  SRR4302532.3934875/94‑1 (MQ=60)
                                                           TACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGC                                 >  SRR4302532.1922214/1‑94 (MQ=60)
                                                           TACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGC                                 >  SRR4302532.5620036/1‑94 (MQ=60)
                                                            ACATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCA                                <  SRR4302532.3590255/94‑1 (MQ=60)
                                                             CATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAG                               <  SRR4302532.454979/94‑1 (MQ=60)
                                                              ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGT                              >  SRR4302532.5966613/1‑94 (MQ=60)
                                                              ATCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATAAGCAGCAGG                              >  SRR4302532.105980/1‑94 (MQ=60)
                                                               TCTGCCTGTTTGTCGCCAAGCTCACGATAATCCTGCGCCACCGCTGTTGCGCCCACCGCTTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTG                             <  SRR4302532.602597/94‑1 (MQ=60)
                                                               TCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTG                             <  SRR4302532.602598/94‑1 (MQ=60)
                                                               TCTGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTG                             <  SRR4302532.2448012/94‑1 (MQ=60)
                                                                 TGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCG                           <  SRR4302532.2175965/94‑1 (MQ=60)
                                                                 TGCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCG                           <  SRR4302532.529450/94‑1 (MQ=60)
                                                                  GCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGTCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGT                          >  SRR4302532.2989582/1‑94 (MQ=60)
                                                                  GCCGGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGT                          >  SRR4302532.5996165/1‑94 (MQ=60)
                                                                     GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCATCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                       <  SRR4302532.5328001/94‑1 (MQ=60)
                                                                     GGTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                       >  SRR4302532.2225716/1‑94 (MQ=60)
                                                                     GGTTTGTCGACAAGCTGACGATAAACCTGCGCCATCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTAT                       <  SRR4302532.5327819/94‑1 (MQ=60)
                                                                      GTTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATC                      >  SRR4302532.4660239/1‑94 (MQ=60)
                                                                       TTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCT                     <  SRR4302532.2925461/94‑1 (MQ=60)
                                                                       TTTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCT                     <  SRR4302532.3846440/94‑1 (MQ=60)
                                                                        TTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCGCAAATCAAGCAGCAGCAGTGCGTTATCTT                    <  SRR4302532.4897801/94‑1 (MQ=60)
                                                                        TTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTT                    <  SRR4302532.448263/94‑1 (MQ=60)
                                                                        TTGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTT                    <  SRR4302532.2370708/94‑1 (MQ=60)
                                                                         TGTCGCCAAGCTGACGATAATCCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTC                   <  SRR4302532.4035397/94‑1 (MQ=60)
                                                                         TGTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTC                   <  SRR4302532.667514/94‑1 (MQ=60)
                                                                          GTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCG                  <  SRR4302532.4659561/94‑1 (MQ=60)
                                                                          GTCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCG                  >  SRR4302532.2393558/1‑94 (MQ=60)
                                                                           TCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCGGTGCGTTATCTTCGG                 <  SRR4302532.3212162/94‑1 (MQ=60)
                                                                           TCGCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGG                 <  SRR4302532.4415623/94‑1 (MQ=60)
                                                                             GCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTA               <  SRR4302532.5293703/94‑1 (MQ=60)
                                                                             GCCAAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTA               >  SRR4302532.1223162/1‑94 (MQ=60)
                                                                                AAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAA            <  SRR4302532.2919003/94‑1 (MQ=60)
                                                                                AAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAA            >  SRR4302532.3972167/1‑94 (MQ=60)
                                                                                AAGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAA            <  SRR4302532.521631/94‑1 (MQ=60)
                                                                                 AGCTGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAA           >  SRR4302532.266860/1‑94 (MQ=60)
                                                                                    TGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCT        <  SRR4302532.3276425/94‑1 (MQ=60)
                                                                                    TGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCT        >  SRR4302532.2494683/1‑94 (MQ=60)
                                                                                    TGACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCT        <  SRR4302532.1370833/94‑1 (MQ=60)
                                                                                     GACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTG       >  SRR4302532.2897088/1‑94 (MQ=60)
                                                                                     GACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTG       >  SRR4302532.4520657/1‑94 (MQ=60)
                                                                                     GACGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTG       >  SRR4302532.5556273/1‑94 (MQ=60)
                                                                                       CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302532.1217487/94‑1 (MQ=60)
                                                                                       CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302532.4125341/94‑1 (MQ=60)
                                                                                       CGATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCG     <  SRR4302532.1349536/94‑1 (MQ=60)
                                                                                        GATAAACCTGCGCCAGCGCCGTTGCGCCCAGTGCGTTATTGCCTTTGCCCAAATGAATCAGCAGCAGTGCGTTATCTTCGTTAGCAAGCTGCTG    >  SRR4302532.5365560/1‑94 (MQ=60)
                                                                                        GATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGG    >  SRR4302532.3275078/1‑94 (MQ=60)
                                                                                        GATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGG    <  SRR4302532.4378052/94‑1 (MQ=60)
                                                                                         ATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC   >  SRR4302532.4901560/1‑94 (MQ=60)
                                                                                         ATAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGC   >  SRR4302532.4166399/1‑94 (MQ=60)
                                                                                          TAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCG  <  SRR4302532.815700/94‑1 (MQ=60)
                                                                                          |                                                                                             
CGCTGTGCAACCAGCGCCTGAATCGCGTCATAGAAGCCTTTCAGTTGCGCGACATCGCGTACATCTGCCGGTTTGTCGCCAAGCTGACGAAAAACCTGCGCCAGCGCCGTTGCGCCCAGCGCGTTATTGCCTTTGCCCAAATCAATCAGCAGCAGTGCGTTATCTTCGGTAGAAAGCTGCGGCG  >  NC_000913/2690934‑2691117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑