Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,766,473 A→G D248G (GAT→GGT)  b2632 → putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,766,4730AG99.4% 611.0 / NA 170D248G (GAT→GGT) b2632putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (78/91);  total (78/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA  >  NC_000913/2766386‑2766561
                                                                                       |                                                                                        
gcagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                                                                     >  2:3173815/1‑93 (MQ=255)
 cagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt                                                                                    <  1:288997/93‑1 (MQ=255)
 cagcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt                                                                                    <  2:3372806/93‑1 (MQ=255)
   gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                                                                  >  1:4121771/1‑93 (MQ=255)
   gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAc                                                                                  <  2:1509656/93‑1 (MQ=255)
     ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                                                                                <  1:4243038/93‑1 (MQ=255)
     ccGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTc                                                                                >  1:5705598/1‑93 (MQ=255)
      cGGTAGTAGCGCTACTGCAACATTCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                                                                               >  1:4751612/1‑93 (MQ=255)
      cGGTAGTAGAGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                                                                               >  1:1695467/1‑93 (MQ=255)
        gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                                                                             <  2:4427883/93‑1 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:2556315/93‑1 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  1:2941609/93‑1 (MQ=255)
         tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  2:1422402/93‑1 (MQ=255)
         tagtagCGCTACGGCAACATCCCGTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                                            <  1:193660/93‑1 (MQ=255)
          agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                                                                           <  2:3921130/93‑1 (MQ=255)
          agtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc                                                                           <  1:1450273/93‑1 (MQ=255)
           gtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATaag                                                                          >  2:4489866/1‑91 (MQ=255)
           gtagCGCTACTGCAACATCACTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg                                                                          <  2:5457595/93‑1 (MQ=255)
            tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                                                                         >  1:1962781/1‑93 (MQ=255)
            tagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACgg                                                                         <  1:1879839/93‑1 (MQ=255)
             agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                                        <  2:2070126/93‑1 (MQ=255)
             agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                                        <  1:596565/93‑1 (MQ=255)
             agCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                                        <  1:2206692/93‑1 (MQ=255)
              gcgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa                                                                       <  2:635169/93‑1 (MQ=255)
              gcgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa                                                                       <  1:5214098/93‑1 (MQ=255)
              gcgcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa                                                                       >  1:4339456/1‑93 (MQ=255)
                gcTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCTATACTCGCTAAGGTAAg                                                                     >  2:835263/1‑93 (MQ=255)
                 cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    >  2:2531854/1‑93 (MQ=255)
                 cTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                                    >  1:1470620/1‑93 (MQ=255)
                  tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGcc                                                                   >  1:4847056/1‑92 (MQ=255)
                  tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCa                                                                   <  1:1272865/93‑1 (MQ=255)
                  tACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCa                                                                   <  2:5211890/93‑1 (MQ=255)
                     tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGGCATACTCGATACGGTAAGCAACt                                                                >  1:2533749/1‑93 (MQ=255)
                     tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                                                <  2:1291659/93‑1 (MQ=255)
                     tGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                                                <  2:1291658/93‑1 (MQ=255)
                     ggCAACATCCCTTTCGAACAACGGGAGCGCGGGAGCAGGCATGTGCCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAATCACCt                                                                <  1:5391932/92‑1 (MQ=255)
                      gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               <  1:4248524/93‑1 (MQ=255)
                      gCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATGTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt                                                               >  1:5475248/1‑93 (MQ=255)
                        aaCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc                                                             >  1:5542120/1‑93 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            <  2:466763/93‑1 (MQ=255)
                         aCATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcc                                                            >  1:511168/1‑93 (MQ=255)
                           aTCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc                                                          >  1:4721223/1‑93 (MQ=255)
                            tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt                                                         >  2:4805727/1‑93 (MQ=255)
                            tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt                                                         >  1:5336168/1‑93 (MQ=255)
                            tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt                                                         >  1:6112528/1‑93 (MQ=255)
                            tCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCt                                                         <  1:742845/93‑1 (MQ=255)
                             cccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCtt                                                        >  1:1168427/1‑93 (MQ=255)
                              ccTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTa                                                       <  2:3614490/93‑1 (MQ=255)
                               cTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAt                                                      >  2:3521558/1‑93 (MQ=255)
                                 ttCGAACAATGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTc                                                    <  1:4163377/93‑1 (MQ=255)
                                         acggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCg                                            <  2:4259754/91‑1 (MQ=255)
                                          cggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCgg                                           <  2:3657028/92‑1 (MQ=255)
                                          cggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCgg                                           <  2:3890588/92‑1 (MQ=255)
                                           ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGt                                          >  1:739502/1‑93 (MQ=255)
                                           ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGt                                          <  2:5359200/93‑1 (MQ=255)
                                           ggTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGt                                          <  2:720256/93‑1 (MQ=255)
                                            gTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTg                                         <  1:3227772/93‑1 (MQ=255)
                                               gCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg                                      >  2:3779553/1‑93 (MQ=255)
                                                 ccGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAc                                    >  1:1859246/1‑93 (MQ=255)
                                                  cGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCTGCCCCGGTGCGGAcc                                   >  2:2232845/1‑93 (MQ=255)
                                                  cGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                                   >  2:5131725/1‑93 (MQ=255)
                                                  cGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                                   >  1:3039592/1‑93 (MQ=255)
                                                  cGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                                   >  1:3903713/1‑93 (MQ=255)
                                                   gggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCa                                  >  2:2065153/1‑93 (MQ=255)
                                                    ggAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat                                 >  1:3920799/1‑93 (MQ=255)
                                                     gAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCatt                                <  1:3578307/93‑1 (MQ=255)
                                                     gAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCAGCCCCGGTGCGGACCatt                                >  2:1665650/1‑93 (MQ=255)
                                                         aGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTc                            >  1:2574824/1‑93 (MQ=255)
                                                          ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           <  2:5714088/93‑1 (MQ=255)
                                                          ggCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCa                           <  1:1644654/93‑1 (MQ=255)
                                                           gCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAg                          <  2:1653363/93‑1 (MQ=255)
                                                           gCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAg                          <  2:4544303/93‑1 (MQ=255)
                                                            cACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAgg                         >  1:1107843/1‑93 (MQ=255)
                                                            cACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAgg                         >  1:3787367/1‑93 (MQ=255)
                                                             aCGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGc                        <  1:2231866/93‑1 (MQ=255)
                                                             aCGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGc                        >  1:2512250/1‑93 (MQ=255)
                                                                tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt                     >  1:4244766/1‑93 (MQ=255)
                                                                tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt                     >  2:5969/1‑93 (MQ=255)
                                                                tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGt                     <  1:5369098/93‑1 (MQ=255)
                                                                tGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGACGt                     >  1:2417367/1‑93 (MQ=255)
                                                                  aCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGc                   >  1:3654210/1‑93 (MQ=255)
                                                                   cGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCg                  >  1:4543292/1‑93 (MQ=255)
                                                                   cGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCg                  >  1:4992614/1‑93 (MQ=255)
                                                                    gATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGt                 >  2:1905988/1‑93 (MQ=255)
                                                                      tttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTc               <  2:1796069/93‑1 (MQ=255)
                                                                       ttCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTcc              <  2:3123398/93‑1 (MQ=255)
                                                                         cGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTc            >  2:2934350/1‑93 (MQ=255)
                                                                          ggTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCg           <  1:2998320/93‑1 (MQ=255)
                                                                            tGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggt         <  1:2172297/93‑1 (MQ=255)
                                                                            ggAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggt         <  1:4606918/92‑1 (MQ=255)
                                                                             gAAACCGTAGGTGCCATACTCGATACGGTAAGCCCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtg        <  1:5786547/93‑1 (MQ=255)
                                                                             gAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtg        >  2:2461605/1‑93 (MQ=255)
                                                                             gAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtg        >  2:747704/1‑93 (MQ=255)
                                                                               aaCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCggtggg      >  1:86217/1‑92 (MQ=255)
                                                                                aCCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGgtggtg     <  1:834318/93‑1 (MQ=255)
                                                                                 ccGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGgtgt    <  2:5460536/93‑1 (MQ=255)
                                                                                 ccGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGCTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGgtgt    >  1:5585689/1‑93 (MQ=255)
                                                                                  cGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTc   <  2:1137160/93‑1 (MQ=255)
                                                                                   gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  <  2:3794222/93‑1 (MQ=255)
                                                                                   gTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCa  >  2:2871103/1‑93 (MQ=255)
                                                                                       |                                                                                        
GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCA  >  NC_000913/2766386‑2766561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGT  >  NC_000913/2766380‑2766559
                                                                                             |                                                                                      
AGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGG                                                                                        <  SRR4302532.5543346/94‑1 (MQ=60)
 GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                                                                       <  SRR4302532.4040331/94‑1 (MQ=60)
 GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                                                                       <  SRR4302532.1908130/94‑1 (MQ=60)
 GGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGT                                                                                       <  SRR4302532.4049574/94‑1 (MQ=60)
      GCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAT                                                                                  <  SRR4302532.1066291/94‑1 (MQ=60)
       CAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATA                                                                                 <  SRR4302532.298965/94‑1 (MQ=60)
        CGCCCGGTAGGAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                                                                                <  SRR4302532.5451136/94‑1 (MQ=60)
        AGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATAC                                                                                >  SRR4302532.4260095/1‑94 (MQ=60)
          CCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTC                                                                              >  SRR4302532.5904530/1‑94 (MQ=60)
           CCGGTAGTAGCGCTACTGCAACATTCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                                                                             >  SRR4302532.4914073/1‑94 (MQ=60)
           CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                                                                             <  SRR4302532.4385590/94‑1 (MQ=60)
           CCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                                                                             >  SRR4302532.1971164/1‑94 (MQ=60)
           CCGGTAGTAGCGCTAATACAACATCCCTTTCGAACAACGGAAGCCCGAGAGCAGGCACGTGACGATTTCGGTGAAACCGTAAAAACAATACTCG                                                                             <  SRR4302532.5684058/94‑1 (MQ=46)
           CCGGTAGTAGAGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCG                                                                             >  SRR4302532.1752700/1‑94 (MQ=60)
            CGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGA                                                                            <  SRR4302532.4312797/94‑1 (MQ=60)
               TAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                         <  SRR4302532.3039281/94‑1 (MQ=60)
               TAGTAGCGCTACGGCAACATCCCGTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAC                                                                         <  SRR4302532.200195/94‑1 (MQ=60)
                AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                                                                        <  SRR4302532.4684419/94‑1 (MQ=60)
                AGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACG                                                                        <  SRR4302532.1499171/94‑1 (MQ=60)
                 GTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGG                                                                       >  SRR4302532.2027963/1‑94 (MQ=60)
                  TAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGT                                                                      <  SRR4302532.1942142/94‑1 (MQ=60)
                   AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                     <  SRR4302532.617394/94‑1 (MQ=60)
                   AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                     <  SRR4302532.3136599/94‑1 (MQ=60)
                   AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                     >  SRR4302532.4486033/1‑94 (MQ=60)
                   AGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTA                                                                     <  SRR4302532.2280061/94‑1 (MQ=60)
                    GCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAA                                                                    <  SRR4302532.5393910/94‑1 (MQ=60)
                     CGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAG                                                                   <  SRR4302532.767207/94‑1 (MQ=60)
                      GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                                                                  >  SRR4302532.2486240/1‑94 (MQ=60)
                      GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                                                                  >  SRR4302532.171173/1‑94 (MQ=60)
                      GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                                                                  >  SRR4302532.1520170/1‑94 (MQ=60)
                      GCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGC                                                                  <  SRR4302532.4890142/94‑1 (MQ=60)
                       CTTCTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACATGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                                                                 <  SRR4302532.5577498/94‑1 (MQ=60)
                       CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCC                                                                 >  SRR4302532.5013908/1‑94 (MQ=60)
                       CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                                                                 <  SRR4302532.5792850/94‑1 (MQ=60)
                       CTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCA                                                                 <  SRR4302532.2989146/94‑1 (MQ=60)
                        TACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCAC                                                                <  SRR4302532.1315441/94‑1 (MQ=60)
                          CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGGCATACTCGATACGGTAAGCAACT                                                              >  SRR4302532.2618278/1‑94 (MQ=60)
                          CTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCT                                                              <  SRR4302532.1846892/94‑1 (MQ=60)
                           TGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATGTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTT                                                             >  SRR4302532.5665084/1‑94 (MQ=60)
                           GGCAACATCCCTTTCGAACAACGGGAGCGCGGGAGCAGGCATGTGCCGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAATCACCTT                                                             <  SRR4302532.5578733/94‑1 (MQ=40)
                            GCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT                                                            <  SRR4302532.4391270/94‑1 (MQ=60)
                             caacatcctttcagaacagcGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC                                                           <  SRR4302532.319544/74‑1 (MQ=60)
                             CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC                                                           >  SRR4302532.5734464/1‑94 (MQ=60)
                             CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC                                                           >  SRR4302532.3190819/1‑94 (MQ=60)
                             CAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTC                                                           >  SRR4302532.4175062/1‑94 (MQ=60)
                              AACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCC                                                          >  SRR4302532.529024/1‑94 (MQ=60)
                                CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC                                                        >  SRR4302532.6359876/1‑94 (MQ=60)
                                CATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCC                                                        >  SRR4302532.4882309/1‑94 (MQ=60)
                                 ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                       >  SRR4302532.6323497/1‑94 (MQ=60)
                                 ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                       >  SRR4302532.5521231/1‑94 (MQ=60)
                                 ATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCT                                                       <  SRR4302532.2023787/94‑1 (MQ=60)
                                  TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTT                                                      >  SRR4302532.1207465/1‑94 (MQ=60)
                                  TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTT                                                      <  SRR4302532.768230/94‑1 (MQ=60)
                                  TCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTT                                                      <  SRR4302532.389418/94‑1 (MQ=60)
                                       ttcgaacaatGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCC                                                 <  SRR4302532.4303002/84‑1 (MQ=60)
                                       ttcgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCC                                                 >  SRR4302532.5551536/11‑94 (MQ=60)
                                        tcgaacaacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCC                                                <  SRR4302532.4043973/85‑1 (MQ=60)
                                             caacGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCC                                           >  SRR4302532.4289682/5‑94 (MQ=60)
                                               cgctgaaccgctcttcgatcttcgggatATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTTCCCTTATTCCCGCCCCGG                                         >  SRR4302532.2848581/29‑94 (MQ=60)
                                                CGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGT                                        >  SRR4302532.764710/1‑94 (MQ=60)
                                                  GTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGC                                      <  SRR4302532.3334907/94‑1 (MQ=60)
                                                      CCCGGGAGCAGGCACGTGACTATTTGGGTGAAACCGTAGGTCCCCTCCTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAC                                  <  SRR4302532.4871045/94‑1 (MQ=60)
                                                      CCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAC                                  <  SRR4302532.1584342/94‑1 (MQ=60)
                                                      CCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAC                                  >  SRR4302532.1920881/1‑94 (MQ=60)
                                                      CCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAC                                  >  SRR4302532.2032449/1‑94 (MQ=60)
                                                       CCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACC                                 >  SRR4302532.3139880/1‑94 (MQ=60)
                                                       CCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACC                                 >  SRR4302532.4035355/1‑94 (MQ=60)
                                                         GGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCAT                               >  SRR4302532.4052884/1‑94 (MQ=60)
                                                         GGGAGCAGGAACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGAAAGCACCTTTACCCTTATTCACGCCCCGGGGCGGACCAT                               >  SRR4302532.531614/1‑94 (MQ=60)
                                                          GGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATT                              >  SRR4302532.2524450/1‑94 (MQ=60)
                                                           GAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTA                             <  SRR4302532.3698025/94‑1 (MQ=60)
                                                             GCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATT                           >  SRR4302532.3578260/1‑94 (MQ=60)
                                                             GCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATT                           <  SRR4302532.3940746/94‑1 (MQ=60)
                                                              CAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                          >  SRR4302532.4453292/1‑94 (MQ=60)
                                                              CAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTC                          >  SRR4302532.2660837/1‑94 (MQ=60)
                                                                GGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAG                        <  SRR4302532.1700371/94‑1 (MQ=60)
                                                                 GCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                       >  SRR4302532.1144883/1‑94 (MQ=60)
                                                                 GCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                       >  SRR4302532.3915133/1‑94 (MQ=60)
                                                                 GCACGTGACCACTTCGGTGAAACGGTAGGTGCCATACCCGATTCGGTAAGCACCTTTGCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                       <  SRR4302532.4910118/94‑1 (MQ=60)
                                                                 GCACGTAAAGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGG                       <  SRR4302532.5389092/94‑1 (MQ=60)
                                                                  CACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGC                      >  SRR4302532.5275546/1‑94 (MQ=60)
                                                                  CACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGC                      >  SRR4302532.2596066/1‑94 (MQ=60)
                                                                   ACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCC                     <  SRR4302532.2305929/94‑1 (MQ=60)
                                                                   ACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCC                     <  SRR4302532.5942941/94‑1 (MQ=60)
                                                                    CGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCG                    >  SRR4302532.1228571/1‑94 (MQ=60)
                                                                     GTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT                   >  SRR4302532.4387386/1‑94 (MQ=60)
                                                                     GTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT                   >  SRR4302532.4387384/1‑94 (MQ=60)
                                                                     GTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGT                   <  SRR4302532.2975191/94‑1 (MQ=60)
                                                                     GTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGACGT                   >  SRR4302532.2497747/1‑94 (MQ=60)
                                                                      TGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTG                  <  SRR4302532.4817372/94‑1 (MQ=60)
                                                                      TGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTG                  <  SRR4302532.5555154/94‑1 (MQ=60)
                                                                       GACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC                 >  SRR4302532.3776877/1‑94 (MQ=60)
                                                                        ACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCG                >  SRR4302532.5163733/1‑94 (MQ=60)
                                                                        ACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCG                >  SRR4302532.4698374/1‑94 (MQ=60)
                                                                         CGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGT               >  SRR4302532.2283027/1‑94 (MQ=60)
                                                                             TTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCT           >  SRR4302532.4945643/1‑94 (MQ=60)
                                                                                GGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGG        <  SRR4302532.3097361/94‑1 (MQ=60)
                                                                                 GTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGT       <  SRR4302532.5608329/94‑1 (MQ=60)
                                                                                  TGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTG      <  SRR4302532.2244905/94‑1 (MQ=60)
                                                                                  GGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTG      <  SRR4302532.4764001/94‑1 (MQ=60)
                                                                                   GAAACCGTAGGTGCCATACTCGATACGGTAAGCCCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGG     <  SRR4302532.5987768/94‑1 (MQ=60)
                                                                                   GAAACCGTAGGTGCCATACTCGATACGGTAAGCCCCTTCCCCCTTATCCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGG     <  SRR4302532.1320475/94‑1 (MQ=60)
                                                                                   GAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGG     <  SRR4302532.2641839/94‑1 (MQ=60)
                                                                                    AAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGT    <  SRR4302532.6028465/94‑1 (MQ=60)
                                                                                    AAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGG    >  SRR4302532.89267/1‑94 (MQ=60)
                                                                                      ACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGT  <  SRR4302532.862931/94‑1 (MQ=60)
                                                                                      ACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGCTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGT  >  SRR4302532.5779606/1‑94 (MQ=60)
                                                                                             |                                                                                      
AGCCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGGACATGGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGATACCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGT  >  NC_000913/2766380‑2766559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑