Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,781,134 T→C D32G (GAC→GGC)  b2648 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,781,1340TC93.8% 651.3 / 22.0 209D32G (GAC→GGC) b2648hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/8);  new base C (119/77);  total (124/85)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 218 total aligned reads displayed.

TTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGC  >  NC_000913/2781042‑2781223
                                                                                            |                                                                                         
ttACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGt                                                                                           <  2:4623407/93‑1 (MQ=255)
  aCATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGccc                                                                                         <  2:3408721/93‑1 (MQ=255)
   cATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGcccc                                                                                        >  1:3236098/1‑93 (MQ=255)
    aTAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCcccc                                                                                       >  1:3679679/1‑93 (MQ=255)
    aTAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCcccc                                                                                       <  1:2021106/93‑1 (MQ=255)
      aaTCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCcccccc                                                                                     >  1:4302900/1‑93 (MQ=255)
      aaTCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCcccccc                                                                                     >  2:5518918/1‑93 (MQ=255)
      aaTCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCcccccc                                                                                     >  1:5449976/1‑93 (MQ=255)
      aaTCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCcccccc                                                                                     >  2:896328/1‑93 (MQ=255)
       aTCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCa                                                                                    >  1:5996430/1‑93 (MQ=255)
        tCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCAt                                                                                   >  1:4208030/1‑93 (MQ=255)
           tttCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCAttgt                                                                                >  2:874000/1‑93 (MQ=255)
           tttCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCAttgt                                                                                >  2:3352734/1‑93 (MQ=255)
           tttCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCAttgt                                                                                >  2:3654614/1‑93 (MQ=255)
           tttCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCAttgt                                                                                >  2:4915974/1‑93 (MQ=255)
             tCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCAttgttg                                                                              <  1:4801131/93‑1 (MQ=255)
              cTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTgg                                                                             <  2:1934914/93‑1 (MQ=255)
               tgatgaATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTggg                                                                            >  2:2852270/1‑93 (MQ=255)
                   gaATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCaaa                                                                        >  2:4044782/1‑93 (MQ=255)
                   gaATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCaaa                                                                        >  2:5017166/1‑93 (MQ=255)
                   gaATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCaaa                                                                        >  2:3103260/1‑93 (MQ=255)
                    tatatCTTCTGCCCACATAAAAATCACACAATAACTTTGAGATCGTAGATTGTGTTACTTTTACAGCATTCGCCCTCCCATTGTTGGGCAAat                                                                       >  1:5494358/2‑93 (MQ=37)
                    aaTATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAat                                                                       >  2:1522413/1‑93 (MQ=255)
                      tatCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAatat                                                                     <  1:1049881/93‑1 (MQ=255)
                      tatCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAatat                                                                     >  1:2484959/1‑93 (MQ=255)
                      tatCTTCTGCTCACATAAAAATCACACAATAACATTGTGATCGCCGATTGTTTTACTTTTACAGCATTCGCCCCCCCCTTGTTGGGCAAataa                                                                     >  1:4451068/1‑92 (MQ=37)
                       atCTTCTGCTCTCATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAAtata                                                                    >  2:781388/1‑93 (MQ=255)
                       atCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAAtata                                                                    <  1:3322333/93‑1 (MQ=255)
                       atCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAAtata                                                                    <  2:845640/93‑1 (MQ=255)
                       atCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAAtata                                                                    >  2:5297277/1‑93 (MQ=255)
                         cttctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGa                                                                  <  2:2742428/93‑1 (MQ=255)
                         cttctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGa                                                                  <  2:1460262/93‑1 (MQ=255)
                         cttctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGa                                                                  <  2:5695374/93‑1 (MQ=255)
                         cttctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGa                                                                  <  2:3974328/93‑1 (MQ=255)
                          ttctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGAt                                                                 >  2:3468928/1‑93 (MQ=255)
                           tctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGAtt                                                                <  1:2708540/93‑1 (MQ=255)
                            ttGCTCACATAAAAATCACACAATAACTTTGAGCTCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTg                                                               <  1:4704759/92‑1 (MQ=255)
                            ctGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTg                                                               <  2:5432946/93‑1 (MQ=255)
                             tGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTgg                                                              >  2:2154672/1‑93 (MQ=255)
                             tGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTgg                                                              >  1:5774690/1‑93 (MQ=255)
                             tGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTgg                                                              >  1:3418248/1‑93 (MQ=255)
                              gCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTggg                                                             >  2:2646047/1‑93 (MQ=255)
                               cTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGc                                                            >  1:4724304/1‑93 (MQ=255)
                                tCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGcc                                                           >  2:1729130/1‑93 (MQ=255)
                                 cacaTAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCa                                                          >  2:793408/1‑93 (MQ=255)
                                  acaTAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCag                                                         >  2:1284838/1‑93 (MQ=255)
                                  acaTAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCag                                                         <  2:3260842/93‑1 (MQ=255)
                                   caTAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCaga                                                        <  2:4562097/93‑1 (MQ=255)
                                    aTAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCagag                                                       >  1:2923872/1‑93 (MQ=255)
                                    aTAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCagag                                                       >  1:3704705/1‑93 (MQ=255)
                                     tAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGc                                                      <  1:2239289/93‑1 (MQ=255)
                                     tAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGc                                                      >  2:5220014/1‑93 (MQ=255)
                                        aaaTCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACg                                                   >  1:5222998/1‑93 (MQ=255)
                                           tCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGaaa                                                <  1:4077519/93‑1 (MQ=255)
                                           tCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGaaa                                                <  2:2574601/93‑1 (MQ=255)
                                           tCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGaaa                                                <  1:121453/93‑1 (MQ=255)
                                           tCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGaaa                                                <  2:169860/93‑1 (MQ=255)
                                           tCACACAATAACTCTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGaaa                                                <  1:3348597/93‑1 (MQ=255)
                                            cacacaATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAg                                               <  2:6077577/93‑1 (MQ=255)
                                             acacaATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGt                                              <  1:2122660/93‑1 (MQ=255)
                                                caATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTaa                                           <  1:1245709/93‑1 (MQ=255)
                                                caATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTaa                                           >  2:2290094/1‑93 (MQ=255)
                                                caATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTaa                                           <  1:1358439/93‑1 (MQ=255)
                                                caATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTaa                                           >  1:776848/1‑93 (MQ=255)
                                                caATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTaa                                           >  2:5928620/1‑93 (MQ=255)
                                                caATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTaa                                           >  2:5558063/1‑93 (MQ=255)
                                                     aCTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCa                                      >  1:6082905/1‑93 (MQ=255)
                                                      cTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCAc                                     <  1:4025902/93‑1 (MQ=255)
                                                       tttGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACg                                    >  2:4267057/1‑93 (MQ=255)
                                                        ttGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGt                                   <  1:1694382/93‑1 (MQ=255)
                                                            gaTCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGc                               >  1:656010/1‑93 (MQ=255)
                                                            gaTCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGc                               >  2:3732395/1‑93 (MQ=255)
                                                               cGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGcaca                            >  2:1163115/1‑93 (MQ=255)
                                                               cGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGcaca                            <  1:180211/93‑1 (MQ=255)
                                                                gCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCCCACAg                           >  2:4394479/1‑93 (MQ=255)
                                                                 cAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGc                          <  1:5082455/93‑1 (MQ=255)
                                                                 cAGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGc                          >  2:4526375/1‑93 (MQ=255)
                                                                 cAGATTGTTTTACTTTGACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGc                          <  1:12820/93‑1 (MQ=255)
                                                                  aGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGct                         >  2:1590650/1‑93 (MQ=255)
                                                                  aGATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGct                         >  1:5929848/1‑93 (MQ=255)
                                                                   gATTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGctc                        >  1:1985498/1‑93 (MQ=255)
                                                                    aTTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGctcc                       >  1:900922/1‑93 (MQ=255)
                                                                    aTTGTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGctcc                       <  1:3538180/93‑1 (MQ=255)
                                                                       gTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCtcctcc                    >  2:3859347/1‑93 (MQ=255)
                                                                       gTTTTACTTTTACAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCtcctcc                    >  1:253384/1‑93 (MQ=255)
                                                                        ttttacttttacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGATCGCACAGCTACTCCa                   >  1:4866007/1‑93 (MQ=255)
                                                                              ttttacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCa             >  1:5700585/1‑93 (MQ=255)
                                                                               tttacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCAc            >  2:3758815/1‑93 (MQ=255)
                                                                               tttacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCAc            >  2:1971623/1‑93 (MQ=255)
                                                                                 tacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGa          >  1:3156550/1‑93 (MQ=255)
                                                                                 tacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGa          >  1:5592691/1‑93 (MQ=255)
                                                                                 tacAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGa          <  2:1224982/93‑1 (MQ=255)
                                                                                  acAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGAc         <  1:926758/93‑1 (MQ=255)
                                                                                   cAGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACa        >  1:2662450/1‑93 (MQ=255)
                                                                                    aGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACaa       >  2:3197957/1‑93 (MQ=255)
                                                                                    aGCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACaa       >  1:435030/1‑93 (MQ=255)
                                                                                     gCATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAg      >  1:1466231/1‑93 (MQ=255)
                                                                                      cATTCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGa     <  1:988975/93‑1 (MQ=255)
                                                                                        ttCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATg   <  2:6103949/93‑1 (MQ=255)
                                                                                         tCGCCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGc  >  2:2955144/1‑93 (MQ=255)
                                                                                            |                                                                                         
TTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGTCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGC  >  NC_000913/2781042‑2781223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGCCACA  >  NC_000913/2781042‑2781227
                                                                                             |                                                                                             
TTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CC                                                                                              >  SRR4302532.1287381/1‑94 (MQ=60)
TTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CC                                                                                              <  SRR4302532.2125981/94‑1 (MQ=60)
  ACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCC                                                                                            >  SRR4302532.3343537/1‑94 (MQ=60)
   CATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCC                                                                                           >  SRR4302532.3803292/1‑94 (MQ=60)
    ATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCC                                                                                          <  SRR4302532.2088481/94‑1 (MQ=60)
    ATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCC                                                                                          >  SRR4302532.5013211/1‑94 (MQ=60)
     TAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCC                                                                                         >  SRR4302532.4448362/1‑94 (MQ=60)
     TAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCC                                                                                         >  SRR4302532.5638873/1‑94 (MQ=60)
      AATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCA                                                                                        >  SRR4302532.6203571/1‑94 (MQ=60)
      AATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCA                                                                                        <  SRR4302532.4013108/94‑1 (MQ=60)
       ATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCAT                                                                                       >  SRR4302532.4349280/1‑94 (MQ=60)
        TCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATT                                                                                      <  SRR4302532.5772838/94‑1 (MQ=60)
         CGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTG                                                                                     >  SRR4302532.2658463/1‑94 (MQ=60)
         CGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTG                                                                                     <  SRR4302532.440791/94‑1 (MQ=60)
          GTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCGCCCCCCCCATTG                                                                                     <  SRR4302532.2066636/94‑1 (MQ=60)
          GTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGT                                                                                    >  SRR4302532.5933433/1‑94 (MQ=60)
           TTTCTGATGAATATCTTCTTCTCACATAAAAATCACACAATAACTTTTAAATCGCATATTTTTTTACGTTTACAGCAATCG‑CCCCCCCATTGTT                                                                                   <  SRR4302532.1178676/94‑1 (MQ=60)
             TCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGG                                                                                 <  SRR4302532.4966483/94‑1 (MQ=60)
              CTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGG                                                                                >  SRR4302532.4192688/1‑94 (MQ=60)
              CTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGG                                                                                >  SRR4302532.2596889/1‑94 (MQ=60)
                GATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCA                                                                              >  SRR4302532.3799881/1‑94 (MQ=60)
                 ATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAA                                                                             >  SRR4302532.5618486/1‑94 (MQ=60)
                  TGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAA                                                                            >  SRR4302532.3492092/1‑94 (MQ=60)
                   GTATATCTTCTGCCCACATAAAAATCACACAATAACTTTGAGATCGTAGATTGTGTTACTTTTACAGCATTCG‑CCCTCCCATTGTTGGGCAAAT                                                                           >  SRR4302532.5684894/1‑94 (MQ=60)
                    AATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATA                                                                          <  SRR4302532.4970288/94‑1 (MQ=60)
                     ATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATAT                                                                         >  SRR4302532.2567747/1‑94 (MQ=60)
                     ATATCTTCTGCTCACATAAAAATCACACAATAACATTGTGATCGCCGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCCTTGTTGGGCAAATAA                                                                         >  SRR4302532.4602768/1‑94 (MQ=60)
                      TATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATA                                                                        <  SRR4302532.1085278/94‑1 (MQ=60)
                       ATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAG                                                                       >  SRR4302532.1536584/1‑94 (MQ=60)
                       ATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAG                                                                       <  SRR4302532.3433109/94‑1 (MQ=60)
                          TTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATT                                                                    <  SRR4302532.781952/94‑1 (MQ=60)
                          TTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATT                                                                    <  SRR4302532.3429516/94‑1 (MQ=60)
                          TTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACGTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATT                                                                    >  SRR4302532.5655269/1‑94 (MQ=60)
                           TCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTG                                                                   <  SRR4302532.2799620/94‑1 (MQ=60)
                            TTGCTCACATAAAAATCACACAATAACTTTGAGCTCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGG                                                                  <  SRR4302532.4865265/94‑1 (MQ=60)
                            CTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGG                                                                  >  SRR4302532.3532748/1‑94 (MQ=60)
                            CTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGG                                                                  >  SRR4302532.5975586/1‑94 (MQ=60)
                            CTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGG                                                                  <  SRR4302532.4865266/94‑1 (MQ=60)
                             TGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGG                                                                 <  SRR4302532.378757/94‑1 (MQ=60)
                              GCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGC                                                                >  SRR4302532.4885513/1‑94 (MQ=60)
                              GCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGC                                                                >  SRR4302532.126904/1‑94 (MQ=60)
                                  ACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGA                                                            <  SRR4302532.4437073/94‑1 (MQ=60)
                                  ACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGA                                                            >  SRR4302532.1373622/1‑94 (MQ=60)
                                  ACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGA                                                            >  SRR4302532.209496/1‑94 (MQ=60)
                                  ACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGA                                                            >  SRR4302532.3267782/1‑94 (MQ=60)
                                   CATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAG                                                           >  SRR4302532.3829070/1‑94 (MQ=60)
                                   CATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAG                                                           >  SRR4302532.3021198/1‑94 (MQ=60)
                                     TAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCA                                                         <  SRR4302532.2313597/94‑1 (MQ=60)
                                     TAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCA                                                         <  SRR4302532.2313596/94‑1 (MQ=60)
                                       AAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACG                                                       >  SRR4302532.5403116/1‑94 (MQ=60)
                                         AATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAA                                                     >  SRR4302532.1229413/1‑94 (MQ=60)
                                           TCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAG                                                   <  SRR4302532.4214436/94‑1 (MQ=60)
                                           TCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAG                                                   <  SRR4302532.125590/94‑1 (MQ=60)
                                           TCACACAATAACTCTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAG                                                   <  SRR4302532.3460395/94‑1 (MQ=60)
                                             ACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTT                                                 <  SRR4302532.2193571/94‑1 (MQ=60)
                                             ACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTT                                                 >  SRR4302532.942193/1‑94 (MQ=60)
                                             ACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTT                                                 >  SRR4302532.2753660/1‑94 (MQ=60)
                                              CACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTA                                                >  SRR4302532.3012116/1‑94 (MQ=60)
                                               ACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAA                                               >  SRR4302532.803591/1‑94 (MQ=60)
                                                CAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAAT                                              <  SRR4302532.2367320/94‑1 (MQ=60)
                                                CAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAAT                                              <  SRR4302532.1287388/94‑1 (MQ=60)
                                                CAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAAT                                              <  SRR4302532.1403924/94‑1 (MQ=60)
                                                    AACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCA                                          >  SRR4302532.6292958/1‑94 (MQ=60)
                                                     ACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCAC                                         >  SRR4302532.2306775/1‑94 (MQ=60)
                                                      CTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACG                                        <  SRR4302532.4161577/94‑1 (MQ=60)
                                                       TTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGT                                       >  SRR4302532.3455465/1‑94 (MQ=60)
                                                        TTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTT                                      <  SRR4302532.1751586/94‑1 (MQ=60)
                                                        TTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTT                                      <  SRR4302532.494592/94‑1 (MQ=60)
                                                         TGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTC                                     <  SRR4302532.655095/94‑1 (MQ=60)
                                                           AGATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGC                                   >  SRR4302532.678742/1‑94 (MQ=60)
                                                             ATCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGAGCCAGAGCACGAAAGTTAATCCCACGTTCGCAC                                 >  SRR4302532.4541550/1‑94 (MQ=60)
                                                              TCGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACA                                <  SRR4302532.6243201/94‑1 (MQ=60)
                                                               CGCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAG                               <  SRR4302532.186273/94‑1 (MQ=60)
                                                                GCAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGC                              <  SRR4302532.907528/94‑1 (MQ=60)
                                                                 CAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCT                             <  SRR4302532.13353/94‑1 (MQ=60)
                                                                 CAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCT                             <  SRR4302532.5257206/94‑1 (MQ=60)
                                                                 CAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCT                             >  SRR4302532.1933642/1‑94 (MQ=60)
                                                                 CAGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCT                             >  SRR4302532.6135128/1‑94 (MQ=60)
                                                                 CAGATTGTTTTACTTTGACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCT                             <  SRR4302532.13354/94‑1 (MQ=60)
                                                                  AGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTC                            >  SRR4302532.2051457/1‑94 (MQ=60)
                                                                  AGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTC                            >  SRR4302532.4303114/1‑94 (MQ=60)
                                                                  AGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTC                            >  SRR4302532.3102757/1‑94 (MQ=60)
                                                                  AGATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTC                            >  SRR4302532.6168007/1‑94 (MQ=60)
                                                                   GATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCC                           <  SRR4302532.4495003/94‑1 (MQ=60)
                                                                   GATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCC                           >  SRR4302532.931640/1‑94 (MQ=60)
                                                                    ATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCT                          <  SRR4302532.1024602/94‑1 (MQ=60)
                                                                    ATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCT                          <  SRR4302532.3626007/94‑1 (MQ=60)
                                                                    ATTGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCT                          <  SRR4302532.3656557/94‑1 (MQ=60)
                                                                      TGTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCC                        >  SRR4302532.262191/1‑94 (MQ=60)
                                                                       GTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCA                       <  SRR4302532.656681/94‑1 (MQ=60)
                                                                       GTTTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGATCGCACAGCTACTCCA                       >  SRR4302532.5033354/1‑94 (MQ=60)
                                                                         TTTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAAC                     >  SRR4302532.576975/1‑94 (MQ=60)
                                                                          TTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACA                    <  SRR4302532.3066610/94‑1 (MQ=60)
                                                                          TTACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTACTCCAACA                    >  SRR4302532.959452/1‑94 (MQ=60)
                                                                            ACTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGC                  >  SRR4302532.149585/1‑94 (MQ=60)
                                                                             CTTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCA                 >  SRR4302532.5899404/1‑94 (MQ=60)
                                                                              TTTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTACAACAGCAC                <  SRR4302532.5877037/94‑1 (MQ=60)
                                                                               TTTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACG               >  SRR4302532.5302232/1‑94 (MQ=60)
                                                                                TTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGA              >  SRR4302532.5786823/1‑94 (MQ=60)
                                                                                TTACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGA              >  SRR4302532.3261321/1‑94 (MQ=60)
                                                                                 TACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGAC             >  SRR4302532.6106532/1‑94 (MQ=60)
                                                                                 TACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATCCCACGTTCGCACAGCTCCTCCAACAGCACGAC             <  SRR4302532.4708626/94‑1 (MQ=60)
                                                                                 TACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTTGCACAGCTCCTCCAACAGCACGAC             >  SRR4302532.5171118/1‑94 (MQ=60)
                                                                                  ACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACA            >  SRR4302532.2751806/1‑94 (MQ=60)
                                                                                  ACAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACA            <  SRR4302532.958504/94‑1 (MQ=60)
                                                                                   CAGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAA           >  SRR4302532.450091/1‑94 (MQ=60)
                                                                                    AGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAG          >  SRR4302532.1684935/1‑94 (MQ=60)
                                                                                    AGCATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAG          >  SRR4302532.1515628/1‑94 (MQ=60)
                                                                                      CATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGAT        <  SRR4302532.1022757/94‑1 (MQ=60)
                                                                                      CATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATAACACGTTCGCACAGCTCCTCCAACAGCACGACAAGAT        <  SRR4302532.3825418/94‑1 (MQ=60)
                                                                                       ATTCG‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATG       >  SRR4302532.1853464/1‑94 (MQ=60)
                                                                                           G‑CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGCCAC   >  SRR4302532.1525660/1‑94 (MQ=60)
                                                                                             CCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGCCACA  <  SRR4302532.4863871/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTTTACTTTTACAGCATTCG‑TCCCCCCATTGTTGGGCAAATATAGATTGGGCCAGAGCACGAAAGTTAATACCACGTTCGCACAGCTCCTCCAACAGCACGACAAGATGCCACA  >  NC_000913/2781042‑2781227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑