Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,797 T→C F128S (TTT→TCT)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,7970TC100.0% 827.9 / NA 230F128S (TTT→TCT) srlEPTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (125/105);  total (125/105)

Only 100 of 234 total aligned reads displayed.

CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCC  >  NC_000913/2824707‑2824885
                                                                                          |                                                                                        
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                        >  1:1436904/1‑93 (MQ=255)
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                        >  1:4792623/1‑93 (MQ=255)
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                        >  1:4251549/1‑93 (MQ=255)
 gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                       <  2:1058137/93‑1 (MQ=255)
   tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                     >  1:2022255/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                   >  1:4966529/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                   <  2:809383/93‑1 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                   <  2:4659218/93‑1 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                 >  1:3634006/1‑93 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                               >  1:108303/1‑93 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                               <  2:2110084/93‑1 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                               >  1:3834624/1‑93 (MQ=255)
           tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                             <  2:4675659/93‑1 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                           <  2:514939/93‑1 (MQ=255)
              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                          <  2:5303931/93‑1 (MQ=255)
               ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                         >  2:3308798/1‑93 (MQ=255)
                 gtgGAAGATATTTATGTCTCTGGCGTACACGAAGCCAACATCACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTAt                                                                       <  1:5458033/93‑1 (MQ=255)
                 gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                       <  2:717371/93‑1 (MQ=255)
                 gtgGAAGATATTTATGACTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTAt                                                                       <  1:5822765/93‑1 (MQ=255)
                  tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                      >  1:557782/1‑93 (MQ=255)
                    gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATgcc                                                                    >  1:3257038/1‑91 (MQ=255)
                    gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                    <  2:304666/93‑1 (MQ=255)
                    gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                    <  1:1941476/93‑1 (MQ=255)
                      aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                                  >  1:3413842/1‑93 (MQ=255)
                      aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGacac                                                                  >  1:3767852/1‑93 (MQ=255)
                       gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                 >  1:798451/1‑93 (MQ=255)
                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                <  1:2206424/93‑1 (MQ=255)
                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                >  1:1580011/1‑93 (MQ=255)
                          atTTATGTCTCTGGCGTAAAAGAAGAAAACTTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                              <  1:3585272/93‑1 (MQ=255)
                            ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                            <  2:3968326/93‑1 (MQ=255)
                            ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                            >  2:4970625/1‑93 (MQ=255)
                             tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                           >  1:4419673/1‑93 (MQ=255)
                              aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                          >  1:2702007/1‑93 (MQ=255)
                                gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                                        >  2:2789538/1‑93 (MQ=255)
                                 tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaaa                                                       >  2:242724/1‑93 (MQ=255)
                                    ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                    >  2:3612846/1‑93 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                   <  1:688717/93‑1 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGACGTGACTATGACACCAGTAAGAAAATCAc                                                   >  1:2708386/1‑93 (MQ=255)
                                     tGGCGTAAAAAAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                   <  2:3625214/93‑1 (MQ=255)
                                      ggCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                                  <  2:5581753/93‑1 (MQ=255)
                                        cGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGa                                                >  1:6026923/1‑93 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCgca                                               >  2:6024298/1‑91 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                               <  2:570380/93‑1 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                               <  1:5683414/93‑1 (MQ=255)
                                          tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                              >  2:2606934/1‑93 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                             >  2:2954377/1‑93 (MQ=255)
                                           aaaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                             >  2:1628937/1‑93 (MQ=255)
                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                            >  1:5979095/1‑93 (MQ=255)
                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                            >  2:5549595/1‑93 (MQ=255)
                                              ataagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                          <  2:1826189/91‑1 (MQ=255)
                                              agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                          <  1:55292/93‑1 (MQ=255)
                                              agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                          >  2:4379743/1‑93 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                         >  2:4496253/1‑93 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                         >  2:542252/1‑93 (MQ=255)
                                               gaagaaAACATCACTGTAGTAGGTAATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                         <  2:3865065/93‑1 (MQ=255)
                                                aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                        >  2:4430929/1‑93 (MQ=255)
                                                  gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                      >  1:374633/1‑93 (MQ=255)
                                                    aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                    >  2:173481/1‑93 (MQ=255)
                                                      aCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                                  <  2:3679203/93‑1 (MQ=255)
                                                      aCATCACTGTAGTAGGTGATGCGACACAACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGtt                                  <  1:1333997/93‑1 (MQ=255)
                                                       cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt                                 <  1:3344270/93‑1 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                >  1:5186892/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                >  2:2995183/1‑93 (MQ=255)
                                                          cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                              <  2:4026002/93‑1 (MQ=255)
                                                           aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                             >  2:1344495/1‑93 (MQ=255)
                                                            cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                            >  1:1646507/1‑93 (MQ=255)
                                                             tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                           <  1:4537519/93‑1 (MQ=255)
                                                              gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAACAACCGAACAAAGCGAGGGTTTACTGGCg                          >  1:3463243/1‑93 (MQ=255)
                                                                agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                        <  2:3419968/93‑1 (MQ=255)
                                                                agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                        <  2:3495993/93‑1 (MQ=255)
                                                                  tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                      >  2:3499797/1‑93 (MQ=255)
                                                                   agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt                     <  1:3361255/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                    >  1:2851655/1‑93 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                    <  1:2629807/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGCAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGTAgggg                    >  1:4584842/1‑91 (MQ=255)
                                                                     gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                   >  2:1906796/1‑93 (MQ=255)
                                                                      tGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTggg                  >  2:3515691/1‑93 (MQ=255)
                                                                       gATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                 >  2:3100849/1‑93 (MQ=255)
                                                                          gCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGACTCCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg              <  2:2834908/93‑1 (MQ=255)
                                                                          gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg              <  1:5628539/93‑1 (MQ=255)
                                                                           cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg             <  1:4655266/93‑1 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg            <  1:4397905/93‑1 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg            <  1:47337/93‑1 (MQ=255)
                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc           >  1:4118325/1‑93 (MQ=255)
                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa          <  2:4886168/93‑1 (MQ=255)
                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa          >  1:1187442/1‑93 (MQ=255)
                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa          <  1:3983517/93‑1 (MQ=255)
                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa          <  1:3115203/93‑1 (MQ=255)
                                                                               accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt         <  2:382585/93‑1 (MQ=255)
                                                                               accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt         >  2:3264968/1‑93 (MQ=255)
                                                                                ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg        <  1:183692/93‑1 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg      <  2:4097060/93‑1 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg      >  2:1846876/1‑93 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg      <  1:5274822/93‑1 (MQ=255)
                                                                                   caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg     >  1:984627/1‑93 (MQ=255)
                                                                                   caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg     <  1:2275452/93‑1 (MQ=255)
                                                                                    aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGt    <  2:4315807/93‑1 (MQ=255)
                                                                                     aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTc   >  2:1202238/1‑93 (MQ=255)
                                                                                     aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTc   >  1:4517340/1‑93 (MQ=255)
                                                                                      cccTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTcc  >  2:1533039/1‑93 (MQ=255)
                                                                                          |                                                                                        
CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCC  >  NC_000913/2824707‑2824885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  NC_000913/2824704‑2824890
                                                                                             |                                                                                             
GTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTC                                                                                               <  SRR4302532.5955643/94‑1 (MQ=60)
  CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTT                                                                                             >  SRR4302532.4957693/1‑94 (MQ=60)
  CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTT                                                                                             >  SRR4302532.4394423/1‑94 (MQ=60)
  CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTT                                                                                             <  SRR4302532.3963491/94‑1 (MQ=60)
  CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTT                                                                                             >  SRR4302532.1485307/1‑94 (MQ=60)
    GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                           <  SRR4302532.4564106/94‑1 (MQ=60)
    CCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                           <  SRR4302532.986906/94‑1 (MQ=60)
     CTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCG                                                                                          >  SRR4302532.2089667/1‑94 (MQ=60)
       GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                        <  SRR4302532.3074825/94‑1 (MQ=60)
       GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                        >  SRR4302532.3628493/1‑94 (MQ=60)
       GGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTG                                                                                        >  SRR4302532.5136826/1‑94 (MQ=60)
         CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      >  SRR4302532.3755913/1‑94 (MQ=60)
         CGCAGTACATTGTGGAAGATATTTATGGCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                      <  SRR4302532.2155493/94‑1 (MQ=60)
          GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                     >  SRR4302532.3879301/1‑94 (MQ=60)
          GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                     >  SRR4302532.323118/1‑94 (MQ=60)
           CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    >  SRR4302532.3964097/1‑94 (MQ=60)
           CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    >  SRR4302532.112033/1‑94 (MQ=60)
           CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                    <  SRR4302532.1247162/94‑1 (MQ=60)
            AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                   >  SRR4302532.4693840/1‑94 (MQ=60)
             GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGT                                                                                  >  SRR4302532.5844335/1‑94 (MQ=60)
              TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACATCACAACCCTCTTCCGTGGGCCGTG                                                                                 >  SRR4302532.5743764/1‑94 (MQ=60)
              TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                 >  SRR4302532.1864569/1‑94 (MQ=60)
              TACATTGCGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                 <  SRR4302532.5221281/94‑1 (MQ=60)
                CATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAC                                                                               <  SRR4302532.811435/94‑1 (MQ=60)
                 ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                              >  SRR4302532.5814176/1‑94 (MQ=60)
                   TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302532.4119657/94‑1 (MQ=60)
                   TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302532.2745692/94‑1 (MQ=60)
                   TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                            <  SRR4302532.2745691/94‑1 (MQ=60)
                    GTGGAAGATATTTATGTCTCTGGCGTACACGAAGCCAACATCACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATG                                                                           <  SRR4302532.5647250/94‑1 (MQ=60)
                    GTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATG                                                                           <  SRR4302532.1137740/94‑1 (MQ=60)
                    GTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATG                                                                           >  SRR4302532.577231/1‑94 (MQ=60)
                    GTGGAAGATATTTATGACTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATG                                                                           <  SRR4302532.6024973/94‑1 (MQ=60)
                      GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGCC                                                                         >  SRR4302532.3365253/1‑94 (MQ=60)
                       GAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACA                                                                        <  SRR4302532.2006029/94‑1 (MQ=60)
                        AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                       >  SRR4302532.3894656/1‑94 (MQ=60)
                        AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                       >  SRR4302532.3528185/1‑94 (MQ=60)
                         AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                      <  SRR4302532.4540655/94‑1 (MQ=60)
                         AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                      >  SRR4302532.2795013/1‑94 (MQ=60)
                         AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                      >  SRR4302532.825746/1‑94 (MQ=60)
                          GATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCA                                                                     >  SRR4302532.1633510/1‑94 (MQ=60)
                           ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                    <  SRR4302532.2279790/94‑1 (MQ=60)
                            TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGA                                                                   >  SRR4302532.1661907/1‑94 (MQ=60)
                             ATTTATGTCTCTGGCGTAAAAGAAGAAAACTTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTA                                                                  <  SRR4302532.3705211/94‑1 (MQ=60)
                             ATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACAACAACCCTCTTCCGAGGGCCGTGACTATGAGACCAGTA                                                                  >  SRR4302532.2064470/1‑94 (MQ=60)
                              TTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                                 >  SRR4302532.5455926/1‑94 (MQ=60)
                               TTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAG                                                                >  SRR4302532.4570220/1‑94 (MQ=60)
                                TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                               >  SRR4302532.2792788/1‑94 (MQ=60)
                                 ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              >  SRR4302532.4912523/1‑94 (MQ=60)
                                 ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                              <  SRR4302532.3735771/94‑1 (MQ=60)
                                   GTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAA                                                            >  SRR4302532.1720951/1‑94 (MQ=60)
                                     CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                          >  SRR4302532.3467691/1‑94 (MQ=60)
                                       CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGACGTGACTATGACACCAGTAAGAAAATCAC                                                        >  SRR4302532.2799459/1‑94 (MQ=60)
                                        TGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACC                                                       <  SRR4302532.5510516/94‑1 (MQ=60)
                                        TGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACC                                                       <  SRR4302532.712346/94‑1 (MQ=60)
                                         GGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCG                                                      >  SRR4302532.5767727/1‑94 (MQ=60)
                                          GCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGA                                                     >  SRR4302532.6235085/1‑94 (MQ=60)
                                           CGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                    >  SRR4302532.6190655/1‑94 (MQ=60)
                                           CGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                    >  SRR4302532.5960105/1‑94 (MQ=60)
                                            GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   >  SRR4302532.4555207/1‑94 (MQ=60)
                                            GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                   <  SRR4302532.5881811/94‑1 (MQ=60)
                                             TAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACATCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAATAAAATCGCCGAACA                                                  <  SRR4302532.3533481/94‑1 (MQ=60)
                                             TAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGAGACCAGTAAGAAAATCACCGAACA                                                  >  SRR4302532.3610036/1‑94 (MQ=60)
                                             TAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACA                                                  >  SRR4302532.477760/1‑94 (MQ=60)
                                              AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                                 >  SRR4302532.6185727/1‑94 (MQ=60)
                                              AAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAA                                                 <  SRR4302532.1481450/94‑1 (MQ=60)
                                               AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                                >  SRR4302532.3044360/1‑94 (MQ=60)
                                               AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                                >  SRR4302532.1408450/1‑94 (MQ=60)
                                                 AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              <  SRR4302532.57270/94‑1 (MQ=60)
                                                 AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              >  SRR4302532.340490/1‑94 (MQ=60)
                                                 AGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              >  SRR4302532.4604602/1‑94 (MQ=60)
                                                 AGAAGAAAACATCACAGTAGTAGGTGATGCGACACCACAACCCTATTCCGGTGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGC                                              <  SRR4302532.3386904/94‑1 (MQ=60)
                                                   AAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGA                                            >  SRR4302532.1922276/1‑94 (MQ=60)
                                                    AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                           >  SRR4302532.387633/1‑94 (MQ=60)
                                                     GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                          <  SRR4302532.4949841/94‑1 (MQ=60)
                                                      AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                         >  SRR4302532.1025204/1‑94 (MQ=60)
                                                      AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                         <  SRR4302532.5367067/94‑1 (MQ=60)
                                                       AAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGT                                        >  SRR4302532.3887301/1‑94 (MQ=60)
                                                         ACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                      <  SRR4302532.4351963/94‑1 (MQ=60)
                                                         ACATCACTGTAGTAGGTGATGCGACACAACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                      <  SRR4302532.1378607/94‑1 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                     >  SRR4302532.5365619/1‑94 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                     <  SRR4302532.6328938/94‑1 (MQ=60)
                                                          CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                     <  SRR4302532.3455889/94‑1 (MQ=60)
                                                           ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                    <  SRR4302532.6185551/94‑1 (MQ=60)
                                                             CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  <  SRR4302532.872203/94‑1 (MQ=60)
                                                             CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                  >  SRR4302532.2932535/1‑94 (MQ=60)
                                                              ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                 <  SRR4302532.4052639/94‑1 (MQ=60)
                                                              ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                 >  SRR4302532.1702291/1‑94 (MQ=60)
                                                               CTGGAGTAGGTGATGCGACACCACCACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATCGGTTACTGGG                                >  SRR4302532.3935795/1‑94 (MQ=60)
                                                                TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                               <  SRR4302532.3527609/94‑1 (MQ=60)
                                                                TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                               <  SRR4302532.4692405/94‑1 (MQ=60)
                                                                TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                               <  SRR4302532.4692404/94‑1 (MQ=60)
                                                                TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAACAACCGAACAAAGCGAGGGTTTACTGGCG                               >  SRR4302532.3579243/1‑94 (MQ=60)
                                                                    GCAGGCGATGCGACACCACAAACCTCTTCCGTGGGCCGTGACTATGAGACCATTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGG                           >  SRR4302532.4572977/1‑94 (MQ=60)
                                                                     TAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGT                          <  SRR4302532.2128646/94‑1 (MQ=60)
                                                                     TAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGT                          <  SRR4302532.3463784/94‑1 (MQ=60)
                                                                     TAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGT                          <  SRR4302532.4136824/94‑1 (MQ=60)
                                                                      AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG                         >  SRR4302532.2946993/1‑94 (MQ=60)
                                                                      AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG                         <  SRR4302532.3473553/94‑1 (MQ=60)
                                                                      AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGCAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGTAGGGG                         >  SRR4302532.4741157/1‑94 (MQ=60)
                                                                       GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                        <  SRR4302532.5882455/94‑1 (MQ=60)
                                                                       GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                        <  SRR4302532.2717896/94‑1 (MQ=60)
                                                                        GTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGG                       <  SRR4302532.259040/94‑1 (MQ=60)
                                                                         TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCCAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                      >  SRR4302532.4707771/1‑94 (MQ=60)
                                                                          GATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAA                     >  SRR4302532.3796443/1‑94 (MQ=60)
                                                                          GATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAA                     >  SRR4302532.4877596/1‑94 (MQ=60)
                                                                             GCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGG                  <  SRR4302532.5823888/94‑1 (MQ=60)
                                                                              CGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGG                 <  SRR4302532.4813973/94‑1 (MQ=60)
                                                                               GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                <  SRR4302532.49009/94‑1 (MQ=60)
                                                                               GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                <  SRR4302532.4547572/94‑1 (MQ=60)
                                                                               GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC                >  SRR4302532.4256546/1‑94 (MQ=60)
                                                                                ACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCA               >  SRR4302532.1227137/1‑94 (MQ=60)
                                                                                 CACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAT              <  SRR4302532.4117705/94‑1 (MQ=60)
                                                                                 CACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAT              <  SRR4302532.3218573/94‑1 (MQ=60)
                                                                                   CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG            <  SRR4302532.189884/94‑1 (MQ=60)
                                                                                    CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG           <  SRR4302532.5656475/94‑1 (MQ=60)
                                                                                     CCAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG          <  SRR4302532.1486444/94‑1 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG          <  SRR4302532.5456719/94‑1 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG          <  SRR4302532.5011228/94‑1 (MQ=60)
                                                                                     ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG          >  SRR4302532.1018255/1‑94 (MQ=60)
                                                                                      CAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGT         <  SRR4302532.2350964/94‑1 (MQ=60)
                                                                                      CAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGT         <  SRR4302532.2350963/94‑1 (MQ=60)
                                                                                       AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTC        >  SRR4302532.4671556/1‑94 (MQ=60)
                                                                                       AACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGAATGGGGTC        >  SRR4302532.64349/1‑94 (MQ=60)
                                                                                             CTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  SRR4302532.189970/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGT  >  NC_000913/2824704‑2824890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑