Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,797 T→C F128S (TTT→TCT)  srlE → PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,7970TC100.0% 977.4 / NA 264F128S (TTT→TCT) srlEPTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (145/119);  total (145/119)

Only 100 of 272 total aligned reads displayed.

CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886
                                                                                          |                                                                                         
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         >  2:4529261/1‑93 (MQ=255)
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         <  2:1841946/93‑1 (MQ=255)
cgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCtctt                                                                                         <  1:2420360/93‑1 (MQ=255)
    ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     <  2:5420967/93‑1 (MQ=255)
    ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     >  2:251642/1‑93 (MQ=255)
     gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    >  2:2593559/1‑93 (MQ=255)
      cgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTgg                                                                                   >  1:2506464/1‑93 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  2:6092112/1‑93 (MQ=255)
       gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:5441532/1‑93 (MQ=255)
        cAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                 >  2:3395114/1‑93 (MQ=255)
        cAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                 >  2:3217663/1‑93 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                >  1:4678601/1‑93 (MQ=255)
         aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAACAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                <  2:1190048/93‑1 (MQ=255)
          gTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCg                                                                               <  1:5733571/93‑1 (MQ=255)
           tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              >  1:2284901/1‑93 (MQ=255)
           tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              >  2:42883/1‑93 (MQ=255)
           tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              >  2:4965356/1‑93 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                            >  1:2376372/1‑93 (MQ=255)
             cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGa                                                                            >  1:687538/1‑93 (MQ=255)
              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           >  2:799970/1‑93 (MQ=255)
              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           <  1:881823/93‑1 (MQ=255)
              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           <  2:1712013/93‑1 (MQ=255)
              aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGCCCGTGAc                                                                           <  1:5371836/93‑1 (MQ=255)
               ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                          >  2:2964207/1‑93 (MQ=255)
                 gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                        <  2:6369807/93‑1 (MQ=255)
                  tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  2:371730/1‑93 (MQ=255)
                  tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  2:5948035/1‑93 (MQ=255)
                   ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      <  2:6251270/93‑1 (MQ=255)
                    gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGac                                                                     <  2:4695684/93‑1 (MQ=255)
                    gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                     <  1:3191558/93‑1 (MQ=255)
                        atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  1:4439581/93‑1 (MQ=255)
                         tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                >  2:203439/1‑93 (MQ=255)
                         tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                >  2:3494486/1‑93 (MQ=255)
                          atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               >  2:3875812/1‑93 (MQ=255)
                          atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               >  2:5460556/1‑93 (MQ=255)
                           tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              <  2:488351/93‑1 (MQ=255)
                           tttATGTCNCTGGCGTAAAAGAAGNAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              <  1:129485/93‑1 (MQ=255)
                            ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTaa                                                             >  2:130792/1‑93 (MQ=255)
                              aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                           <  2:4574456/93‑1 (MQ=255)
                              aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGa                                                           >  1:1070051/1‑93 (MQ=255)
                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  1:6409567/1‑93 (MQ=255)
                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          <  1:203186/93‑1 (MQ=255)
                               tGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  1:2775102/1‑93 (MQ=255)
                                gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaaa                                                         >  2:4801728/1‑93 (MQ=255)
                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:6134063/93‑1 (MQ=255)
                                  ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  2:4618262/1‑93 (MQ=255)
                                   tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      <  1:2180949/93‑1 (MQ=255)
                                   tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:265594/1‑93 (MQ=255)
                                     tGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                    >  2:2173852/1‑93 (MQ=255)
                                      ggCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                                   <  2:2231880/93‑1 (MQ=255)
                                       gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcca                                                  >  2:1540472/1‑92 (MQ=255)
                                       gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                                                  >  1:1836221/1‑93 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                >  1:352399/1‑93 (MQ=255)
                                         gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                >  2:4535143/1‑93 (MQ=255)
                                            aaaGAAGAAAACATTACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             >  2:2693264/1‑93 (MQ=255)
                                            aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                             <  1:4554198/93‑1 (MQ=255)
                                              agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           >  1:5243193/1‑93 (MQ=255)
                                                aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                         >  1:1526036/1‑93 (MQ=255)
                                                aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                         <  1:4996533/93‑1 (MQ=255)
                                                 agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                        >  2:643302/1‑93 (MQ=255)
                                                 agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGa                                        <  2:5118885/93‑1 (MQ=255)
                                                  gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       >  1:2712954/1‑93 (MQ=255)
                                                  aaaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       <  1:1154839/92‑1 (MQ=255)
                                                    aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                     >  1:6286269/1‑93 (MQ=255)
                                                     aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGACCAAAGCGATGGt                                    <  1:1828029/93‑1 (MQ=255)
                                                     aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    <  1:3297263/93‑1 (MQ=255)
                                                       cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGttt                                  >  1:1552308/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCTGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  1:4937944/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  1:3068033/1‑93 (MQ=255)
                                                        aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 >  1:2533427/1‑93 (MQ=255)
                                                         tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                >  1:2608540/1‑93 (MQ=255)
                                                          cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               >  2:6000857/1‑93 (MQ=255)
                                                           aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                              >  1:1671190/1‑93 (MQ=255)
                                                           aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                              >  1:6463105/1‑93 (MQ=255)
                                                           aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                              <  2:4501985/93‑1 (MQ=255)
                                                           aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                              >  2:4415307/1‑93 (MQ=255)
                                                            cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             >  2:394669/1‑93 (MQ=255)
                                                             tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:4266146/93‑1 (MQ=255)
                                                             tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:4232436/93‑1 (MQ=255)
                                                              gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           <  2:5296259/93‑1 (MQ=255)
                                                               gagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGa                          <  2:1007959/92‑1 (MQ=255)
                                                                agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                         <  2:498535/93‑1 (MQ=255)
                                                                  tagGTGATGCGACACCACAACCCTCTGCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       <  2:726208/93‑1 (MQ=255)
                                                                   agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt                      >  2:40676/1‑93 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     <  1:4085120/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     >  1:4095350/1‑93 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     <  1:5133416/93‑1 (MQ=255)
                                                                    ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     >  2:2072780/1‑93 (MQ=255)
                                                                        aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:5575034/93‑1 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGCATggg             >  1:1635865/1‑93 (MQ=255)
                                                                            gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             >  2:6576145/1‑93 (MQ=255)
                                                                             acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc            >  2:1421003/1‑93 (MQ=255)
                                                                              caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           <  2:4002077/93‑1 (MQ=255)
                                                                                ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg         <  1:3851409/93‑1 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  2:4352442/93‑1 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  1:6109212/93‑1 (MQ=255)
                                                                                  acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  2:5252301/93‑1 (MQ=255)
                                                                                   caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGTATGGGCATgggg      >  2:5652270/1‑93 (MQ=255)
                                                                                      cccTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTcc   <  2:5907297/93‑1 (MQ=255)
                                                                                       ccTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCa  >  1:5600519/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824707‑2824886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG  >  NC_000913/2824705‑2824889
                                                                                            |                                                                                            
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCT                                                                                             >  SRR4302547.5190582/1‑94 (MQ=60)
 CCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCCCAACCCTCTT                                                                                            <  SRR4302547.2109580/94‑1 (MQ=60)
  CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTC                                                                                           >  SRR4302547.4175934/1‑94 (MQ=60)
  CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTC                                                                                           >  SRR4302547.790007/1‑94 (MQ=60)
  CGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTC                                                                                           <  SRR4302547.2469355/94‑1 (MQ=60)
   GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCC                                                                                          <  SRR4302547.5067650/94‑1 (MQ=60)
     TGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGT                                                                                        >  SRR4302547.6485558/1‑94 (MQ=60)
     TGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGT                                                                                        <  SRR4302547.5166232/94‑1 (MQ=60)
     TGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGT                                                                                        >  SRR4302547.3873609/1‑94 (MQ=60)
       GCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGG                                                                                      <  SRR4302547.1126679/94‑1 (MQ=60)
       GCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGG                                                                                      <  SRR4302547.1439179/94‑1 (MQ=60)
       GCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGG                                                                                      >  SRR4302547.2557130/1‑94 (MQ=60)
        CGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGG                                                                                     >  SRR4302547.5553977/1‑94 (MQ=60)
         GCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGC                                                                                    >  SRR4302547.3298010/1‑94 (MQ=60)
          CAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCC                                                                                   >  SRR4302547.4775515/1‑94 (MQ=60)
           AGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCG                                                                                  >  SRR4302547.5065845/1‑94 (MQ=60)
            GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGT                                                                                 >  SRR4302547.1003750/1‑94 (MQ=60)
            GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGT                                                                                 >  SRR4302547.2331160/1‑94 (MQ=60)
            GTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGT                                                                                 <  SRR4302547.5852027/94‑1 (MQ=60)
             TACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTG                                                                                <  SRR4302547.6439567/94‑1 (MQ=60)
              ACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGA                                                                               >  SRR4302547.2424515/1‑94 (MQ=60)
              ACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGA                                                                               >  SRR4302547.700226/1‑94 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                             <  SRR4302547.2572173/94‑1 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACT                                                                             <  SRR4302547.898769/94‑1 (MQ=60)
                ATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGCCCGTGACT                                                                             <  SRR4302547.5482696/94‑1 (MQ=60)
                  TGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAT                                                                           <  SRR4302547.493194/94‑1 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACACTCTTCCGTGGGCCGTGACTATGA                                                                         >  SRR4302547.3136019/1‑94 (MQ=60)
                    TGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTAATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGA                                                                         <  SRR4302547.3393141/94‑1 (MQ=60)
                     GGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGAC                                                                        >  SRR4302547.5697918/1‑94 (MQ=60)
                      GAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACA                                                                       <  SRR4302547.3255151/94‑1 (MQ=60)
                      GAAGATATTTATGTCTCTGGCATAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACA                                                                       <  SRR4302547.5729391/94‑1 (MQ=60)
                       AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                      <  SRR4302547.763740/94‑1 (MQ=60)
                       AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                      >  SRR4302547.5532364/1‑94 (MQ=60)
                       AAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAC                                                                      >  SRR4302547.2226578/1‑94 (MQ=60)
                        AGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACC                                                                     >  SRR4302547.1287449/1‑94 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                   <  SRR4302547.5427783/94‑1 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                   <  SRR4302547.4531380/94‑1 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                   <  SRR4302547.4016433/94‑1 (MQ=60)
                          ATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAG                                                                   >  SRR4302547.1792033/1‑94 (MQ=60)
                           TATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGT                                                                  <  SRR4302547.6590753/94‑1 (MQ=60)
                             TTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                                >  SRR4302547.1338700/1‑94 (MQ=60)
                             TTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                                <  SRR4302547.1865293/94‑1 (MQ=60)
                             TTTATGTCNCTGGCGTAAAAGAAGNAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAA                                                                <  SRR4302547.131824/94‑1 (MQ=60)
                               TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                              >  SRR4302547.2550499/1‑94 (MQ=60)
                               TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                              >  SRR4302547.1815340/1‑94 (MQ=60)
                               TATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGA                                                              >  SRR4302547.1091361/1‑94 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                             <  SRR4302547.1890786/94‑1 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                             >  SRR4302547.6543235/1‑94 (MQ=60)
                                ATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAA                                                             >  SRR4302547.2830691/1‑94 (MQ=60)
                                 TGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAA                                                            <  SRR4302547.206987/94‑1 (MQ=60)
                                  GTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAA                                                           >  SRR4302547.1677034/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                         >  SRR4302547.457339/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                         >  SRR4302547.270562/1‑94 (MQ=60)
                                    CTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATC                                                         <  SRR4302547.6261310/94‑1 (MQ=60)
                                     TCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCA                                                        <  SRR4302547.624210/94‑1 (MQ=60)
                                     TCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCA                                                        <  SRR4302547.2224634/94‑1 (MQ=60)
                                      CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAC                                                       >  SRR4302547.261955/1‑94 (MQ=60)
                                      CTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAC                                                       <  SRR4302547.3547879/94‑1 (MQ=60)
                                       TGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACC                                                      <  SRR4302547.2764526/94‑1 (MQ=60)
                                        GGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCG                                                     >  SRR4302547.1872521/1‑94 (MQ=60)
                                         GCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGCAAGAAAATAACCGA                                                    >  SRR4302547.1677524/1‑94 (MQ=60)
                                          CGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAA                                                   >  SRR4302547.358946/1‑94 (MQ=60)
                                           GTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAC                                                  >  SRR4302547.5696644/1‑94 (MQ=60)
                                            TAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACA                                                 <  SRR4302547.5550604/94‑1 (MQ=60)
                                              AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                               >  SRR4302547.2455356/1‑94 (MQ=60)
                                              AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                               >  SRR4302547.4615104/1‑94 (MQ=60)
                                              AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAA                                               <  SRR4302547.4648403/94‑1 (MQ=60)
                                              AAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACACTCTTCCGTGGGCCGTGACTATGACATCAGTAAGAAAATCAACGAAGAAA                                               >  SRR4302547.65988/1‑94 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACGCCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                              <  SRR4302547.2534432/94‑1 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                              >  SRR4302547.3939243/1‑94 (MQ=60)
                                               AAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAG                                              >  SRR4302547.5351109/1‑94 (MQ=60)
                                                 GAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCG                                            >  SRR4302547.1556406/1‑94 (MQ=60)
                                                 GAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCG                                            <  SRR4302547.6202512/94‑1 (MQ=60)
                                                  AAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGA                                           <  SRR4302547.5099853/94‑1 (MQ=60)
                                                   AGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAT                                          >  SRR4302547.2767414/1‑94 (MQ=60)
                                                    GAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                         <  SRR4302547.2122958/94‑1 (MQ=60)
                                                    AAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATG                                         <  SRR4302547.1178207/94‑1 (MQ=60)
                                                     AAAACATCACTGTAGTAGGTGATGCGACACCACCACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                        <  SRR4302547.6784356/94‑1 (MQ=60)
                                                     AAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGG                                        >  SRR4302547.6416881/1‑94 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGACCAAAGCGATGGTT                                      <  SRR4302547.1864163/94‑1 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTT                                      >  SRR4302547.5406832/1‑94 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTT                                      >  SRR4302547.2821843/1‑94 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTT                                      >  SRR4302547.4428527/1‑94 (MQ=60)
                                                       AACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTT                                      <  SRR4302547.3363587/94‑1 (MQ=60)
                                                        ACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                     >  SRR4302547.1583204/1‑94 (MQ=60)
                                                        ACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                     >  SRR4302547.2959041/1‑94 (MQ=60)
                                                        ACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTT                                     <  SRR4302547.2365228/94‑1 (MQ=60)
                                                         CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCTGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                    >  SRR4302547.5040218/1‑94 (MQ=60)
                                                         CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                    >  SRR4302547.3129017/1‑94 (MQ=60)
                                                         CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                    >  SRR4302547.1562941/1‑94 (MQ=60)
                                                         CATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTA                                    >  SRR4302547.2584632/1‑94 (MQ=60)
                                                          ATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAC                                   >  SRR4302547.2661116/1‑94 (MQ=60)
                                                           TCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACT                                  <  SRR4302547.1656295/94‑1 (MQ=60)
                                                           TCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACT                                  <  SRR4302547.2600746/94‑1 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                 >  SRR4302547.1704112/1‑94 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                 <  SRR4302547.2921957/94‑1 (MQ=60)
                                                            CACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTG                                 >  SRR4302547.6597693/1‑94 (MQ=60)
                                                             ACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGG                                <  SRR4302547.2698301/94‑1 (MQ=60)
                                                              CTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGC                               >  SRR4302547.6361350/1‑94 (MQ=60)
                                                               TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                              >  SRR4302547.196984/1‑94 (MQ=60)
                                                               TGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCG                              >  SRR4302547.2161050/1‑94 (MQ=60)
                                                                 TAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAA                            <  SRR4302547.2453940/94‑1 (MQ=60)
                                                                    TAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGG                         >  SRR4302547.1631536/1‑94 (MQ=60)
                                                                     AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG                        >  SRR4302547.4178628/1‑94 (MQ=60)
                                                                     AGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTG                        >  SRR4302547.5730579/1‑94 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                       <  SRR4302547.5682926/94‑1 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                       <  SRR4302547.4168149/94‑1 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                       <  SRR4302547.5239356/94‑1 (MQ=60)
                                                                      GGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGG                       <  SRR4302547.5239187/94‑1 (MQ=60)
                                                                       GTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGG                      <  SRR4302547.6268175/94‑1 (MQ=60)
                                                                        TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                     >  SRR4302547.5108985/1‑94 (MQ=60)
                                                                        TGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGA                     >  SRR4302547.1211402/1‑94 (MQ=60)
                                                                         GATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAA                    >  SRR4302547.2362067/1‑94 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                   <  SRR4302547.6018523/94‑1 (MQ=60)
                                                                          ATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAAT                   <  SRR4302547.5689988/94‑1 (MQ=60)
                                                                           TGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATG                  <  SRR4302547.4250765/94‑1 (MQ=60)
                                                                            GCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGG                 <  SRR4302547.2691766/94‑1 (MQ=60)
                                                                            GCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGG                 <  SRR4302547.486776/94‑1 (MQ=60)
                                                                             CGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGCATGGG                >  SRR4302547.1668212/1‑94 (MQ=60)
                                                                              GACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGC               <  SRR4302547.913441/94‑1 (MQ=60)
                                                                                  CCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGG           <  SRR4302547.3929807/94‑1 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG          <  SRR4302547.4707225/94‑1 (MQ=60)
                                                                                   CACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGG          <  SRR4302547.3621709/94‑1 (MQ=60)
                                                                                    ACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGG         <  SRR4302547.6235931/94‑1 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA     >  SRR4302547.5716048/1‑94 (MQ=60)
                                                                                        CCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA     >  SRR4302547.279122/1‑94 (MQ=60)
                                                                                         CCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCAC    >  SRR4302547.5774691/1‑94 (MQ=60)
                                                                                         CCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCAC    <  SRR4302547.319924/94‑1 (MQ=60)
                                                                                         CCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGGCCAC    >  SRR4302547.3864920/1‑94 (MQ=60)
                                                                                          CTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACC   <  SRR4302547.82710/94‑1 (MQ=60)
                                                                                           TCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG  >  SRR4302547.4191481/1‑94 (MQ=60)
                                                                                            |                                                                                            
TCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCG  >  NC_000913/2824705‑2824889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑