Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,077,640 +G intergenic (‑291/+23) cmtB ← / ← tktA PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,077,6401.G100.0% 580.8 / NA 164intergenic (‑291/+23)cmtB/tktAPTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (142/22);  total (142/22)

Only 100 of 178 total aligned reads displayed.

TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC  >  NC_000913/3077548‑3077726
                                                                                             |                                                                                      
 attGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                                        >  2:1225074/1‑92 (MQ=255)
 attGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                                        >  1:4272826/1‑92 (MQ=255)
 attGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                                        >  2:4796419/1‑92 (MQ=255)
  ttGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcga                                                                                       >  2:823534/1‑91 (MQ=255)
  ttGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcga                                                                                       >  2:1420435/1‑91 (MQ=255)
      atatAAATATTTGTGATCTACAACACGCCTTATCTGTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                   >  1:6082413/1‑93 (MQ=255)
      atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                   >  2:2020099/1‑93 (MQ=255)
      atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                   >  2:3849469/1‑93 (MQ=255)
      atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                   >  1:2652387/1‑93 (MQ=255)
       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                  <  1:3505482/93‑1 (MQ=255)
       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                  >  1:3200423/1‑93 (MQ=255)
       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGACGAAGCGACCttt                                                                                  >  2:376222/1‑93 (MQ=255)
        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 >  1:5428918/1‑93 (MQ=255)
        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 >  2:1114812/1‑93 (MQ=255)
        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 >  2:5392888/1‑93 (MQ=255)
        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 <  2:5038902/93‑1 (MQ=255)
         taAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt                                                                                >  1:1460401/1‑93 (MQ=255)
           aaTATTTGTTATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAc                                                                              >  2:3158618/1‑93 (MQ=255)
           aaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAc                                                                              >  1:4456312/1‑93 (MQ=255)
           aaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAc                                                                              >  2:2553697/1‑93 (MQ=255)
            atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAcc                                                                             >  1:159498/1‑93 (MQ=255)
            atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAcc                                                                             >  2:3010252/1‑93 (MQ=255)
            atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAcc                                                                             >  2:4630658/1‑93 (MQ=255)
            atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAcc                                                                             >  1:898730/1‑93 (MQ=255)
             tatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAccc                                                                            >  2:4310009/1‑93 (MQ=255)
              atTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCg                                                                           >  2:4647696/1‑93 (MQ=255)
               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGa                                                                          >  1:5434038/1‑93 (MQ=255)
               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGa                                                                          >  1:4779081/1‑93 (MQ=255)
                ttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaa                                                                         >  2:2446262/1‑93 (MQ=255)
                ttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaa                                                                         >  1:1136185/1‑93 (MQ=255)
                 tgtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaaa                                                                        >  2:4247603/1‑93 (MQ=255)
                  gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGGCATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAt                                                                       >  1:712246/1‑93 (MQ=255)
                  gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAt                                                                       <  2:396798/93‑1 (MQ=255)
                      tCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAATAGGTCGCCGAAGCGACCTTTTTACCTCAAATGCTa                                                                   >  1:2397317/1‑93 (MQ=255)
                      tCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTa                                                                   <  1:3691970/93‑1 (MQ=255)
                      tCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTa                                                                   >  1:2985543/1‑93 (MQ=255)
                       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                                  <  1:2020099/93‑1 (MQ=255)
                       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                                  <  2:208969/93‑1 (MQ=255)
                        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                                 >  2:5752126/1‑93 (MQ=255)
                        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                                 >  2:1491895/1‑93 (MQ=255)
                        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                                 >  1:210923/1‑93 (MQ=255)
                        tACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAt                                                                 >  1:5854986/1‑93 (MQ=255)
                         acaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACGCGAAATGCTAAtt                                                                >  1:3076588/1‑93 (MQ=255)
                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTaa                                                              >  2:2187477/1‑92 (MQ=255)
                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                              >  2:3514155/1‑93 (MQ=255)
                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                              >  2:5559546/1‑93 (MQ=255)
                            acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAca                                                             >  2:671697/1‑93 (MQ=255)
                            acacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAca                                                             >  2:2596966/1‑93 (MQ=255)
                             cacGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcag                                                            >  2:6126572/1‑93 (MQ=255)
                              acGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagc                                                           >  2:3806507/1‑93 (MQ=255)
                               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagca                                                          <  2:2838507/93‑1 (MQ=255)
                                 ccTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGt                                                        >  2:6102110/1‑93 (MQ=255)
                                  cTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGtt                                                       >  1:3251536/1‑93 (MQ=255)
                                  cTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGtt                                                       >  1:2885408/1‑93 (MQ=255)
                                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTc                                                      >  1:5391299/1‑93 (MQ=255)
                                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTc                                                      >  1:372433/1‑93 (MQ=255)
                                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAACTACAGCAGATc                                                      >  1:5479249/1‑93 (MQ=255)
                                    tATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCt                                                     >  2:4407983/1‑93 (MQ=255)
                                     aTCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtt                                                    >  2:593117/1‑93 (MQ=255)
                                      tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTCATTACAGCAGTTCttt                                                   >  1:2495573/1‑93 (MQ=255)
                                      tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCttt                                                   >  2:2659323/1‑93 (MQ=255)
                                       cTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtttt                                                  >  1:5686409/1‑93 (MQ=255)
                                        tATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTg                                                 >  1:3633402/1‑93 (MQ=255)
                                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                                                >  2:2232242/1‑93 (MQ=255)
                                          ttGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTGTTTTGCt                                               >  1:2137704/1‑93 (MQ=255)
                                          ttGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCt                                               >  2:5798134/1‑93 (MQ=255)
                                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                           >  1:1873231/1‑93 (MQ=255)
                                              ttGTCCGCAAACGGACATATCAAGGAAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGATTTCg                                           >  2:1503902/1‑93 (MQ=255)
                                               tGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGc                                          >  2:2259539/1‑93 (MQ=255)
                                               tGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGc                                          >  2:5055123/1‑93 (MQ=255)
                                                  ccGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaac                                       >  1:4899479/1‑93 (MQ=255)
                                                    gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                                     >  1:2190410/1‑93 (MQ=255)
                                                    gCAAACGGACACATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                                     <  2:3463093/93‑1 (MQ=255)
                                                     cAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCaacaac                                    >  2:4956389/1‑93 (MQ=255)
                                                      aaaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACg                                   >  1:759038/1‑93 (MQ=255)
                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  >  1:5145744/1‑93 (MQ=255)
                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  >  1:4943041/1‑93 (MQ=255)
                                                         cGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTa                                >  1:4162086/1‑93 (MQ=255)
                                                           gACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                              <  2:5670516/93‑1 (MQ=255)
                                                             cATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCaa                            >  2:156970/1‑93 (MQ=255)
                                                              atatCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc                           >  1:2246652/1‑93 (MQ=255)
                                                               tataAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa                          >  1:1817427/1‑93 (MQ=255)
                                                                atCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAg                         >  2:4326983/1‑93 (MQ=255)
                                                                 tCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt                        >  1:1152133/1‑93 (MQ=255)
                                                                 tCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt                        >  2:2810904/1‑93 (MQ=255)
                                                                   aaGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                      >  2:5505397/1‑93 (MQ=255)
                                                                   aaGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                      >  1:4704051/1‑93 (MQ=255)
                                                                    aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  2:5121205/1‑93 (MQ=255)
                                                                     ggTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg                    >  1:4557061/1‑93 (MQ=255)
                                                                       taataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGcc                  >  2:1634946/1‑93 (MQ=255)
                                                                       taataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGcc                  >  2:4417996/1‑93 (MQ=255)
                                                                        aataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCg                 >  2:173017/1‑93 (MQ=255)
                                                                        aataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCg                 >  1:3963462/1‑93 (MQ=255)
                                                                          taaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGaa               >  1:1822556/1‑93 (MQ=255)
                                                                           aaaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAc              >  2:5225021/1‑93 (MQ=255)
                                                                           aaaaaaCGTCCCCGAAGCTGCCTTTTTTCGCGAAATGGTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAc              <  2:1201763/93‑1 (MQ=255)
                                                                              aaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActct           >  1:666828/1‑93 (MQ=255)
                                                                                   tCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCaaa      >  2:1753755/1‑93 (MQ=255)
                                                                                      ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcag   >  1:3426779/1‑93 (MQ=255)
                                                                                       cGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcagc  >  2:2364067/1‑93 (MQ=255)
                                                                                             |                                                                                      
TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC  >  NC_000913/3077548‑3077726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  NC_000913/3077547‑3077733
                                                                                             |                                                                                              
TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                >  SRR4302532.3190087/1‑94 (MQ=60)
TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                <  SRR4302532.1498839/94‑1 (MQ=60)
TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                >  SRR4302532.695669/1‑94 (MQ=60)
 TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑G                                                                                              >  SRR4302532.4416688/1‑94 (MQ=60)
     GCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT                                                                                           >  SRR4302532.861410/1‑94 (MQ=60)
     GCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT                                                                                           >  SRR4302532.2194285/1‑94 (MQ=60)
      CATATAAATATTTGTGATCTACAACACGCCTTATCTGTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTT                                                                                          >  SRR4302532.6292448/1‑94 (MQ=60)
      CATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTT                                                                                          >  SRR4302532.2741390/1‑94 (MQ=60)
      CATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTT                                                                                          >  SRR4302532.5790197/1‑94 (MQ=60)
       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302532.3306675/1‑94 (MQ=60)
        TATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTGCGCAAACGGACGTATCAAGGTAATAAAAAAGGTCGCCGAAGCgagctttt                                                                                        >  SRR4302532.1114254/1‑86 (MQ=60)
        TATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTT                                                                                        <  SRR4302532.3622890/94‑1 (MQ=60)
        TATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTT                                                                                        >  SRR4302532.5617072/1‑94 (MQ=60)
         ATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTT                                                                                       >  SRR4302532.5123647/1‑94 (MQ=60)
         ATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTT                                                                                       >  SRR4302532.1509624/1‑94 (MQ=60)
          TAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTA                                                                                      >  SRR4302532.741027/1‑94 (MQ=60)
           AAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAC                                                                                     >  SRR4302532.4608202/1‑94 (MQ=60)
            AATATTTGTGGTCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACC                                                                                    >  SRR4302532.4254601/1‑94 (MQ=60)
            AATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACC                                                                                    >  SRR4302532.164807/1‑94 (MQ=60)
            AATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACC                                                                                    >  SRR4302532.164806/1‑94 (MQ=60)
            AATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACC                                                                                    >  SRR4302532.929377/1‑94 (MQ=60)
              TATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCATGGTAATAAAAATGGTCGCCGAAGCGACCTTTTTACCCG                                                                                  >  SRR4302532.5664866/1‑94 (MQ=60)
               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302532.5622368/1‑94 (MQ=60)
               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302532.4943666/1‑94 (MQ=60)
                TTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAA                                                                                >  SRR4302532.1174084/1‑94 (MQ=60)
                  TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGGCATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAT                                                                              >  SRR4302532.736580/1‑94 (MQ=60)
                    TGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGC                                                                            <  SRR4302532.2153881/94‑1 (MQ=60)
                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAATAGGTCGCCGAAGCGACCTTTTTACCTCAAATGCTA                                                                          >  SRR4302532.2477142/1‑94 (MQ=60)
                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTA                                                                          >  SRR4302532.3084250/1‑94 (MQ=60)
                       TCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAA                                                                         <  SRR4302532.3815923/94‑1 (MQ=60)
                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        >  SRR4302532.6058033/1‑94 (MQ=60)
                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        >  SRR4302532.218122/1‑94 (MQ=60)
                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        <  SRR4302532.2087434/94‑1 (MQ=60)
                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        >  SRR4302532.5896700/1‑94 (MQ=60)
                         TACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACGCGAAATGCTAATT                                                                       >  SRR4302532.3178252/1‑94 (MQ=60)
                         TACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATT                                                                       >  SRR4302532.5474926/1‑94 (MQ=60)
                              CACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGC                                                                  >  SRR4302532.1926948/1‑94 (MQ=60)
                               ACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCgaccttatcccgaagatcggaagagcgtcgt                                                                 >  SRR4302532.5551890/1‑63 (MQ=60)
                               ACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCA                                                                 >  SRR4302532.4407035/1‑94 (MQ=60)
                                 GCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGT                                                               >  SRR4302532.6339196/1‑94 (MQ=60)
                                  CCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTT                                                              >  SRR4302532.2981827/1‑94 (MQ=60)
                                  CCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTT                                                              >  SRR4302532.3359519/1‑94 (MQ=60)
                                  CCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTT                                                              >  SRR4302532.3662121/1‑94 (MQ=60)
                                   CTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTC                                                             >  SRR4302532.5578082/1‑94 (MQ=60)
                                   CTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTC                                                             >  SRR4302532.385371/1‑94 (MQ=60)
                                   CTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAACTACAGCAGATC                                                             >  SRR4302532.5669223/1‑94 (MQ=60)
                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            >  SRR4302532.2067240/1‑94 (MQ=60)
                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            >  SRR4302532.1429685/1‑94 (MQ=60)
                                     TATCTATTGCTTGTCCGCAAACGCACATATCAAGGTAATAAAAAACGTCGCCGAAGCGAACTTTTTACCCGAAATGCTCCTTAAAGCAGTTCTT                                                           >  SRR4302532.4261136/1‑94 (MQ=60)
                                      ATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTCATTACAGCAGTTCTTT                                                          >  SRR4302532.2578773/1‑94 (MQ=60)
                                       TCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTT                                                         >  SRR4302532.5884887/1‑94 (MQ=60)
                                        CTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTG                                                        >  SRR4302532.3755288/1‑94 (MQ=60)
                                          ATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTGTTTTGCT                                                      >  SRR4302532.2209097/1‑94 (MQ=60)
                                           TTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTT                                                     >  SRR4302532.2823815/1‑94 (MQ=60)
                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302532.1935272/1‑94 (MQ=60)
                                                  TCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAAC                                              >  SRR4302532.5067857/1‑94 (MQ=60)
                                                   CCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACA                                             >  SRR4302532.5108742/1‑94 (MQ=60)
                                                   CCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACA                                             <  SRR4302532.4771904/94‑1 (MQ=60)
                                                    CGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAA                                            >  SRR4302532.2263427/1‑94 (MQ=60)
                                                    CGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTGTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAA                                            >  SRR4302532.3891882/1‑94 (MQ=60)
                                                      CAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACG                                          >  SRR4302532.785115/1‑94 (MQ=60)
                                                       AAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGT                                         >  SRR4302532.5322761/1‑94 (MQ=60)
                                                       AAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGT                                         >  SRR4302532.5112702/1‑94 (MQ=60)
                                                         ACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTA                                       >  SRR4302532.4301680/1‑94 (MQ=60)
                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     >  SRR4302532.4890170/1‑94 (MQ=60)
                                                            GACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCA                                    >  SRR4302532.3453808/1‑94 (MQ=60)
                                                              CATATCAAGGTAATAAAAAAGGTCGCCGAAGCTTCCTTTTTGCCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAC                                  <  SRR4302532.4629283/94‑1 (MQ=60)
                                                              CATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAC                                  >  SRR4302532.2321213/1‑94 (MQ=60)
                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302532.794813/1‑94 (MQ=60)
                                                               ATATAAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302532.1878004/1‑94 (MQ=60)
                                                                 ATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAGCGTTATCAcaagt                               >  SRR4302532.2033686/1‑89 (MQ=60)
                                                                 ATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGT                               >  SRR4302532.1190577/1‑94 (MQ=60)
                                                                   CAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGA                             >  SRR4302532.4864540/1‑94 (MQ=60)
                                                                     AGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAG                           >  SRR4302532.230999/1‑94 (MQ=60)
                                                                     AGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAG                           >  SRR4302532.4712534/1‑94 (MQ=60)
                                                                      GGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGCTATCAACAGTGAAGC                          >  SRR4302532.3080107/1‑94 (MQ=60)
                                                                       GTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCC                         >  SRR4302532.2206037/1‑94 (MQ=60)
                                                                        TAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCG                        >  SRR4302532.4096998/1‑94 (MQ=60)
                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302532.1883267/1‑94 (MQ=60)
                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302532.1612016/1‑94 (MQ=60)
                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302532.382996/1‑94 (MQ=60)
                                                                              AAAAGGTCGCCGACGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCT                  >  SRR4302532.2340974/1‑94 (MQ=60)
                                                                              AAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCT                  >  SRR4302532.689852/1‑94 (MQ=60)
                                                                               AAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTT                 <  SRR4302532.3128861/94‑1 (MQ=60)
                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTC                >  SRR4302532.3584754/1‑94 (MQ=60)
                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAAAGTTATCAACAGTGAAGCCGAACTCTTC                >  SRR4302532.2477873/1‑94 (MQ=60)
                                                                                      GCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAG          >  SRR4302532.3541587/1‑94 (MQ=60)
                                                                                       CCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC         >  SRR4302532.5601450/1‑94 (MQ=60)
                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        >  SRR4302532.1133011/1‑94 (MQ=60)
                                                                                         GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAG       <  SRR4302532.4690523/94‑1 (MQ=60)
                                                                                          AAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGC      >  SRR4302532.1979828/1‑94 (MQ=60)
                                                                                          AAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGC      <  SRR4302532.5316995/94‑1 (MQ=60)
                                                                                          AAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTATGCTTTCGCAACAACGTTATCAACAGTGAATCCGAAATCTTCAAACAGCAGC      >  SRR4302532.1979829/1‑94 (MQ=60)
                                                                                           AGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCT     >  SRR4302532.6048756/1‑94 (MQ=60)
                                                                                           AGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCT     >  SRR4302532.6048757/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.599547/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.5945032/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.5475505/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.1498484/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.47228/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.3890582/1‑94 (MQ=60)
                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302532.3708875/1‑94 (MQ=60)
                                                                                             |                                                                                              
TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  NC_000913/3077547‑3077733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑