Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,107,469 +C intergenic (‑296/‑101) b2965 ← / → yqgA ornithine decarboxylase isozyme/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,4691.C100.0% 825.6 / NA 231intergenic (‑296/‑101)b2965/yqgAornithine decarboxylase isozyme/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (102/129);  total (102/129)

Only 100 of 254 total aligned reads displayed.

GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA  >  NC_000913/3107377‑3107558
                                                                                             |                                                                                         
gAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCttt                                                                                            >  1:851833/1‑93 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtttc                                                                                           >  2:5958494/1‑92 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtttc                                                                                           >  2:2095873/1‑92 (MQ=255)
  aaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtttcg                                                                                          <  1:389496/93‑3 (MQ=255)
  aaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtttcg                                                                                          <  2:5561137/93‑3 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAg                                                                                    >  1:1726352/1‑93 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGc                                                                                   >  2:1225663/1‑93 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGc                                                                                   <  1:4187736/93‑1 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGc                                                                                   <  2:5355016/93‑1 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCg                                                                                  <  2:737564/93‑1 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCg                                                                                  >  2:2521808/1‑93 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCg                                                                                  >  1:1433253/1‑93 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCg                                                                                  >  1:6120269/1‑93 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCg                                                                                  <  1:113749/93‑1 (MQ=255)
           aaGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGt                                                                                 <  1:604136/93‑1 (MQ=255)
           aaGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGt                                                                                 <  2:387495/93‑1 (MQ=255)
           aaGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGt                                                                                 <  2:3525615/93‑1 (MQ=255)
            aGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTg                                                                                <  2:5444391/93‑1 (MQ=255)
             gCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGc                                                                               <  2:252770/93‑1 (MQ=255)
              cGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCa                                                                              <  1:5081561/93‑1 (MQ=255)
              cGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCa                                                                              <  1:3378751/93‑1 (MQ=255)
               gagagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCaa                                                                             <  2:3920337/93‑1 (MQ=255)
                agagaATGTAAGGAGATGGCGTGGATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGTGTGCAAg                                                                            >  1:4367320/1‑93 (MQ=255)
                agagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAg                                                                            >  1:1303032/1‑93 (MQ=255)
                 gagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATTGCACCTTTCGCTAAAGCGTGCAAGc                                                                           <  1:2549521/93‑1 (MQ=255)
                   gaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCa                                                                         <  1:2356710/93‑1 (MQ=255)
                     aTGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAgg                                                                       >  1:772022/1‑93 (MQ=255)
                      tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGa                                                                      <  2:3167928/93‑1 (MQ=255)
                          aGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACttt                                                                  <  2:345086/93‑1 (MQ=255)
                           ggAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTg                                                                 >  2:2300862/1‑93 (MQ=255)
                            gagaTGGCGGGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTCGc                                                                <  1:4755071/93‑1 (MQ=255)
                              gaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGa                                                              <  2:1126157/93‑1 (MQ=255)
                                tGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                            <  1:670707/93‑1 (MQ=255)
                                tGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                            >  2:5796682/1‑93 (MQ=255)
                                tGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                            >  2:1410282/1‑93 (MQ=255)
                                   cGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGAGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaa                                                         <  2:1591505/93‑1 (MQ=255)
                                    gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaac                                                        >  1:4971735/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                       >  2:6012362/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                       >  2:3501874/1‑93 (MQ=255)
                                      gCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGGAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAacaaacaa                                                      <  2:2789872/93‑1 (MQ=255)
                                        aTCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGc                                                    >  2:1553261/1‑93 (MQ=255)
                                         tCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGcc                                                   <  2:2225731/93‑1 (MQ=255)
                                         tCGGGAAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGcc                                                   <  1:2790895/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCTCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                                 <  2:2647509/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                                 <  1:2292846/93‑1 (MQ=255)
                                            ggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATc                                                <  2:538540/93‑1 (MQ=255)
                                            ggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATc                                                <  2:1033457/93‑1 (MQ=255)
                                             gCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCg                                               <  2:4384790/93‑1 (MQ=255)
                                             gCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCg                                               >  1:2841849/1‑93 (MQ=255)
                                              cAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGa                                              <  2:1897874/93‑1 (MQ=255)
                                              cAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGa                                              <  1:1765553/93‑1 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                             >  2:1987729/1‑93 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                             >  2:89297/1‑93 (MQ=255)
                                                 aCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACgc                                           >  1:4875690/1‑92 (MQ=255)
                                                  cTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGc                                          <  2:649582/93‑1 (MQ=255)
                                                   tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCa                                         >  1:646758/1‑93 (MQ=255)
                                                   tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCa                                         >  2:2337516/1‑93 (MQ=255)
                                                    ccGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAc                                        <  2:5051474/93‑1 (MQ=255)
                                                    ccGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAc                                        <  2:1772717/93‑1 (MQ=255)
                                                     cGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAcc                                       >  1:1260996/1‑93 (MQ=255)
                                                     cGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAcc                                       >  2:4737528/1‑93 (MQ=255)
                                                      gTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCa                                      <  2:514612/93‑1 (MQ=255)
                                                       tACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAGAAACAAGCCATCGACGGCACCaa                                     >  1:3497764/1‑93 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                   <  1:2974233/93‑1 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                   <  1:2734385/93‑1 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                   <  1:4644910/93‑1 (MQ=255)
                                                          agagagGAGAAAATTCGCGCAATCATTGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACg                                  <  1:4510557/93‑1 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                                 <  1:5675059/93‑1 (MQ=255)
                                                            agagGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGt                                <  2:3008679/93‑1 (MQ=255)
                                                            agagGAAAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGt                                <  1:1358286/93‑1 (MQ=255)
                                                             gaggagAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTc                               >  2:2324249/1‑93 (MQ=255)
                                                              aggagAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCa                              >  1:3779594/1‑93 (MQ=255)
                                                              aggagAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCa                              <  2:5694342/93‑1 (MQ=255)
                                                               ggagAAAATTCGCGCAATCATGGCACTTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCat                             >  1:1887896/1‑93 (MQ=255)
                                                               ggagAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCat                             >  1:3996391/1‑93 (MQ=255)
                                                               ggagAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCat                             <  1:199149/93‑1 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                            <  1:5725116/93‑1 (MQ=255)
                                                                  gaAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataat                          <  1:3068422/93‑1 (MQ=255)
                                                                   aaaaTTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataata                         <  1:1068096/93‑1 (MQ=255)
                                                                    aaaTTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAtaataa                        <  1:1017137/93‑1 (MQ=255)
                                                                     aaTTCGCGCAATCATGGCACCTTTCGATAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAg                       <  2:5250067/93‑1 (MQ=255)
                                                                          gcgcAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt                  >  1:1500919/1‑93 (MQ=255)
                                                                          gcgcAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt                  >  2:4061414/1‑93 (MQ=255)
                                                                          gcgcAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt                  >  1:3959078/1‑93 (MQ=255)
                                                                           cgcAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATc                 <  2:1256132/93‑1 (MQ=255)
                                                                              aaTCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa              <  1:210524/93‑1 (MQ=255)
                                                                               aTCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc             >  1:1441300/1‑93 (MQ=255)
                                                                               aTCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc             >  2:1094755/1‑93 (MQ=255)
                                                                                tCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa            <  1:3265178/93‑1 (MQ=255)
                                                                                tCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa            >  2:2030476/1‑93 (MQ=255)
                                                                                gcATGACACCTTTCGCTAAAGCGTCCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa            <  1:552856/92‑1 (MQ=255)
                                                                                 cATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACaa           >  2:484326/1‑93 (MQ=255)
                                                                                 cATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACaa           <  2:4489444/93‑1 (MQ=255)
                                                                                   tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa         <  2:1485995/93‑1 (MQ=255)
                                                                                   tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa         <  1:2589175/93‑1 (MQ=255)
                                                                                   tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa         <  1:1175520/93‑1 (MQ=255)
                                                                                   tGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa         <  1:4087780/93‑1 (MQ=255)
                                                                                    ggCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAc        <  1:2211137/93‑1 (MQ=255)
                                                                                     gCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa       <  2:3793173/93‑1 (MQ=255)
                                                                                      cACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAg      <  1:5515003/93‑1 (MQ=255)
                                                                                             |                                                                                         
GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA  >  NC_000913/3107377‑3107558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTT‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562
                                                                                              |                                                                                              
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTT                                                                                                >  SRR4302532.881134/1‑94 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTcg                                                                                              >  SRR4302532.2381849/1‑92 (MQ=60)
   AACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTcgc                                                                                             <  SRR4302532.403115/94‑4 (MQ=60)
     CGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTA                                                                                           >  SRR4302532.1721480/1‑94 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAACCTCCGTACAGAGAGGAGAAAATTAGCGCCATCTTGGCACC‑TTTCGCTAAAG                                                                                        >  SRR4302532.3425179/1‑94 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAG                                                                                        >  SRR4302532.1784481/1‑94 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGC                                                                                       <  SRR4302532.4284575/94‑1 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCG                                                                                      >  SRR4302532.6331479/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCG                                                                                      <  SRR4302532.4328234/94‑1 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCG                                                                                      >  SRR4302532.1481509/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCG                                                                                      >  SRR4302532.1481507/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGGACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCG                                                                                      >  SRR4302532.1481508/1‑94 (MQ=60)
           TAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGT                                                                                     <  SRR4302532.117655/94‑1 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTG                                                                                    <  SRR4302532.625242/94‑1 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTG                                                                                    <  SRR4302532.1555882/94‑1 (MQ=60)
               CGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAA                                                                                 >  SRR4302532.542327/1‑94 (MQ=60)
               CGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAA                                                                                 <  SRR4302532.71076/94‑1 (MQ=60)
               CGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAA                                                                                 <  SRR4302532.5256281/94‑1 (MQ=60)
               CGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAA                                                                                 <  SRR4302532.3491741/94‑1 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGGATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGTGTGCAAG                                                                                >  SRR4302532.4515465/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAG                                                                                >  SRR4302532.4515464/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAG                                                                                >  SRR4302532.1346644/1‑94 (MQ=60)
                 AGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGC                                                                               >  SRR4302532.4844843/1‑94 (MQ=60)
                 AGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGC                                                                               >  SRR4302532.3310563/1‑94 (MQ=60)
                 AGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGC                                                                               >  SRR4302532.134076/1‑94 (MQ=60)
                  GAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATTGCACC‑TTTCGCTAAAGCGTGCAAGCC                                                                              <  SRR4302532.2634553/94‑1 (MQ=60)
                  GAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCC                                                                              >  SRR4302532.285239/1‑94 (MQ=60)
                    GAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAG                                                                            <  SRR4302532.2435333/94‑1 (MQ=60)
                    GAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAG                                                                            >  SRR4302532.4038196/1‑94 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302532.587431/94‑1 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302532.2740679/94‑1 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGG                                                                           >  SRR4302532.798660/1‑94 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGAC                                                                         >  SRR4302532.302827/1‑94 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGAC                                                                         >  SRR4302532.2653322/1‑94 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGAC                                                                         >  SRR4302532.2653321/1‑94 (MQ=60)
                        GTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACT                                                                        >  SRR4302532.1351415/1‑94 (MQ=60)
                        GTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACT                                                                        >  SRR4302532.2086709/1‑94 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      >  SRR4302532.6255127/1‑94 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      <  SRR4302532.1795780/94‑1 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      >  SRR4302532.2625459/1‑94 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     >  SRR4302532.802952/1‑94 (MQ=60)
                            agagatgggcgGCATCGGGCAAACCCCGTAAAGAGGAGAAAAAATTCGCCCAATTATGGCCCC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    <  SRR4302532.1493548/83‑1 (MQ=27)
                            GGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    >  SRR4302532.315751/1‑94 (MQ=60)
                            GGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    >  SRR4302532.5709487/1‑94 (MQ=60)
                             GAGATGGCGGGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTCGCG                                                                   <  SRR4302532.4917710/94‑1 (MQ=60)
                               GATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAA                                                                 <  SRR4302532.2041451/94‑1 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                >  SRR4302532.2977926/1‑94 (MQ=60)
                                 TGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACA                                                               <  SRR4302532.693831/94‑1 (MQ=60)
                                    CGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAAC                                                            >  SRR4302532.5142177/1‑94 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       <  SRR4302532.1398084/94‑1 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       >  SRR4302532.514433/1‑94 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       <  SRR4302532.5727706/94‑1 (MQ=60)
                                          TCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCA                                                      >  SRR4302532.1842355/1‑94 (MQ=60)
                                          TCGGGAAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCA                                                      <  SRR4302532.2884468/94‑1 (MQ=60)
                                           CGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAT                                                     <  SRR4302532.4159284/94‑1 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    <  SRR4302532.2368954/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   <  SRR4302532.3460050/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   <  SRR4302532.755900/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   >  SRR4302532.2936841/1‑94 (MQ=60)
                                              GCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGA                                                  >  SRR4302532.2903170/1‑94 (MQ=60)
                                               CAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAC                                                 <  SRR4302532.1869306/94‑1 (MQ=60)
                                               CAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAC                                                 <  SRR4302532.1824725/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302532.2266626/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302532.1495490/94‑1 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGC                                               >  SRR4302532.5043314/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             <  SRR4302532.5988768/94‑1 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             >  SRR4302532.669211/1‑94 (MQ=60)
                                                     CCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACC                                           <  SRR4302532.3816670/94‑1 (MQ=60)
                                                     CCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACC                                           >  SRR4302532.1303180/1‑94 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          <  SRR4302532.3778502/94‑1 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          >  SRR4302532.1583614/1‑94 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          >  SRR4302532.4183952/1‑94 (MQ=60)
                                                       GTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAGAAACAAGCCATCGACGGCACCAA                                         >  SRR4302532.3614955/1‑94 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302532.1197031/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302532.3072648/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      >  SRR4302532.6146431/1‑94 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302532.4803277/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302532.2826292/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGAGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302532.4020120/94‑1 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATTATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     >  SRR4302532.4127323/1‑94 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATCATTGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     <  SRR4302532.4664559/94‑1 (MQ=60)
                                                            GAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGT                                    >  SRR4302532.5632566/1‑94 (MQ=60)
                                                            GAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGT                                    <  SRR4302532.5873093/94‑1 (MQ=60)
                                                             AGAGGAAAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   <  SRR4302532.1403766/94‑1 (MQ=60)
                                                              GAGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCA                                  >  SRR4302532.3906932/1‑94 (MQ=60)
                                                               AGGAGAAAATTCGCGCAATCATGGCACT‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAT                                 >  SRR4302532.1950490/1‑94 (MQ=60)
                                                               AGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAACGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAT                                 <  SRR4302532.4401501/94‑1 (MQ=60)
                                                               AGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAT                                 >  SRR4302532.4131017/1‑94 (MQ=60)
                                                               AGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAT                                 <  SRR4302532.5734131/94‑1 (MQ=60)
                                                               AGGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAT                                 <  SRR4302532.5004224/94‑1 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                <  SRR4302532.3549050/94‑1 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                <  SRR4302532.205948/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302532.1153712/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302532.5924509/94‑1 (MQ=60)
                                                                  AGAAAATTGGCTCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              <  SRR4302532.836400/94‑1 (MQ=60)
                                                                   GAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATA                             <  SRR4302532.3169811/94‑1 (MQ=60)
                                                                   GAAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATA                             >  SRR4302532.204653/1‑94 (MQ=60)
                                                                    AAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAA                            <  SRR4302532.1226489/94‑1 (MQ=60)
                                                                    AAAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAA                            <  SRR4302532.1104002/94‑1 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACC‑TTTCGGTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           >  SRR4302532.2429556/1‑94 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           >  SRR4302532.4945285/1‑94 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302532.5116990/94‑1 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302532.1051663/94‑1 (MQ=60)
                                                                      AATTCGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCGAGCCAGGACTTTGCGAACAAGCAAGCCATCGACGGCACCAAACGGTCATTATAAGA                          >  SRR4302532.5378169/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      >  SRR4302532.4092480/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      >  SRR4302532.1551568/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      >  SRR4302532.4092479/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      >  SRR4302532.4796511/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      <  SRR4302532.2318129/94‑1 (MQ=60)
                                                                           GCGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATC                     <  SRR4302532.689648/94‑1 (MQ=60)
                                                                            CGCAATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCA                    >  SRR4302532.1252187/1‑94 (MQ=60)
                                                                               AATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 <  SRR4302532.217703/94‑1 (MQ=60)
                                                                               AATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 >  SRR4302532.1489883/1‑94 (MQ=60)
                                                                                ATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                >  SRR4302532.1101757/1‑94 (MQ=60)
                                                                                ATCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                >  SRR4302532.4703282/1‑94 (MQ=60)
                                                                                 TCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.5175291/94‑1 (MQ=60)
                                                                                 TCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.2256619/94‑1 (MQ=60)
                                                                                 TCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.4595635/94‑1 (MQ=60)
                                                                                 TCATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.3373766/94‑1 (MQ=60)
                                                                                 GCATGACACC‑TTTCGCTAAAGCGTCCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.572141/94‑1 (MQ=60)
                                                                                 ACATGGCACCGTTC‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.638315/94‑1 (MQ=60)
                                                                                 ACATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302532.638314/94‑1 (MQ=60)
                                                                                   ATGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCCCCAAACGGTCATAATAAGAAAATCAACCAATA             >  SRR4302532.3353225/1‑94 (MQ=60)
                                                                                    TGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            <  SRR4302532.1214794/94‑1 (MQ=60)
                                                                                    TGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            <  SRR4302532.4224999/94‑1 (MQ=60)
                                                                                    TGGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            <  SRR4302532.2675726/94‑1 (MQ=60)
                                                                                     GGCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           <  SRR4302532.2284616/94‑1 (MQ=60)
                                                                                      GCACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302532.1134116/94‑1 (MQ=60)
                                                                                       CACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         >  SRR4302532.2826419/1‑94 (MQ=60)
                                                                                       CACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         <  SRR4302532.5706385/94‑1 (MQ=60)
                                                                                        ACC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCA        >  SRR4302532.2249668/1‑94 (MQ=60)
                                                                                         CC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       <  SRR4302532.6108368/94‑1 (MQ=60)
                                                                                         CC‑TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       <  SRR4302532.5812391/94‑1 (MQ=60)
                                                                                          C‑TTC‑GCTAAAGCGTGCAAGCCAGGACTGTGCGAACAAACAAGCCATCGACTGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     <  SRR4302532.1465511/94‑1 (MQ=60)
                                                                                             TC‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAG   <  SRR4302532.3096786/94‑1 (MQ=60)
                                                                                              C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302532.300533/1‑94 (MQ=60)
                                                                                              C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302532.1999501/1‑94 (MQ=60)
                                                                                              C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302532.1586783/1‑94 (MQ=60)
                                                                                              C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  <  SRR4302532.5610976/94‑1 (MQ=60)
                                                                                              |                                                                                              
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑TTT‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑