Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,107,469 +C intergenic (‑296/‑101) b2965 ← / → yqgA ornithine decarboxylase isozyme/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,4691.C100.0% 848.8 / NA 234intergenic (‑296/‑101)b2965/yqgAornithine decarboxylase isozyme/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (108/126);  total (108/126)

Only 100 of 269 total aligned reads displayed.

GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC  >  NC_000913/3107377‑3107555
                                                                                              |                                                                                      
gAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑t                                                                                         >  1:1333125/1‑93 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tc                                                                                        <  1:5130865/93‑2 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tc                                                                                        >  2:3363983/1‑92 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tc                                                                                        >  2:6417376/1‑92 (MQ=255)
   aCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcgc                                                                                      <  1:867280/93‑4 (MQ=255)
   aCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcgc                                                                                      >  1:36105/1‑90 (MQ=255)
   aCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcgc                                                                                      <  2:6068035/93‑4 (MQ=255)
      gCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATAATGGCACCTT‑TCGCTaa                                                                                   <  1:3170061/93‑1 (MQ=255)
       cGATAAGCGAGAGAATGTCAGGCGACGGCGTGCCTCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTaaa                                                                                  <  2:15843/93‑1 (MQ=255)
       cGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTaaa                                                                                  <  2:4621620/93‑1 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAg                                                                                 <  2:2881745/93‑1 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAg                                                                                 >  1:1886311/1‑93 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGTGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGc                                                                                <  2:1690349/93‑1 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGc                                                                                <  2:3983471/93‑1 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TAGCTAAAGc                                                                                >  1:6631140/1‑93 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCg                                                                               >  2:2994823/1‑93 (MQ=255)
           aaGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGt                                                                              >  2:6207316/1‑93 (MQ=255)
           aaGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGt                                                                              >  1:5227861/1‑93 (MQ=255)
             gCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGc                                                                            >  2:5679443/1‑93 (MQ=255)
             gCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGc                                                                            <  2:5434686/93‑1 (MQ=255)
              cGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCa                                                                           <  2:311089/93‑1 (MQ=255)
               gagagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCaa                                                                          <  1:3344171/93‑1 (MQ=255)
               gagagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCaa                                                                          >  2:5247613/1‑93 (MQ=255)
                agagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAg                                                                         >  1:2333028/1‑93 (MQ=255)
                agagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAg                                                                         >  1:1598095/1‑93 (MQ=255)
                agagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAg                                                                         <  2:588543/93‑1 (MQ=255)
                 gagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTCAAGCGTGCAAGc                                                                        >  1:1764999/1‑93 (MQ=255)
                 gagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGc                                                                        >  2:5630820/1‑93 (MQ=255)
                  agaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGcc                                                                       <  2:5039658/93‑1 (MQ=255)
                   gaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCa                                                                      >  1:4034015/1‑93 (MQ=255)
                    aaTGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAg                                                                     <  1:1949832/93‑1 (MQ=255)
                      tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGa                                                                   <  1:1724483/93‑1 (MQ=255)
                         aaGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACtt                                                                <  1:4371479/93‑1 (MQ=255)
                           ggAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTg                                                              >  2:6350819/1‑93 (MQ=255)
                              gaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGa                                                           <  2:59517/93‑1 (MQ=255)
                               aTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaa                                                          <  1:3997277/93‑1 (MQ=255)
                                tGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                         <  2:2561912/93‑1 (MQ=255)
                                 ggCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaaca                                                        >  1:4923834/1‑93 (MQ=255)
                                  gCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaa                                                       >  2:1929111/1‑93 (MQ=255)
                                   cGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaa                                                      <  2:686249/93‑1 (MQ=255)
                                    gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaac                                                     <  2:3846820/93‑1 (MQ=255)
                                    gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaac                                                     <  1:6454992/93‑1 (MQ=255)
                                    gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaac                                                     >  1:504835/1‑93 (MQ=255)
                                      gCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAacaaacaa                                                   <  2:4454816/93‑1 (MQ=255)
                                        aTCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGc                                                 >  1:1492920/1‑93 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              <  1:6295062/93‑1 (MQ=255)
                                              cAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGa                                           <  1:4448463/93‑1 (MQ=255)
                                              cAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGa                                           <  1:3827213/93‑1 (MQ=255)
                                              cAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGa                                           >  1:5472680/1‑93 (MQ=255)
                                               aagcTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  2:6324547/90‑1 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         >  1:6221958/1‑93 (MQ=255)
                                                 aCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACgg                                        >  1:941559/1‑93 (MQ=255)
                                                  cTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGc                                       <  2:3829255/93‑1 (MQ=255)
                                                    ccGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAc                                     <  1:1249720/93‑1 (MQ=255)
                                                    ccGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAGCGACGGCAc                                     <  1:1094193/93‑1 (MQ=255)
                                                     cGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAcc                                    >  2:778199/1‑93 (MQ=255)
                                                       tACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaa                                  <  2:5281474/93‑1 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGCCTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 >  1:1277457/1‑93 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaac                                 >  2:2045326/1‑92 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 >  1:4713305/1‑93 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 <  1:22444/93‑1 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                <  1:2745487/93‑1 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                <  2:4128207/93‑1 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                <  1:768344/93‑1 (MQ=255)
                                                          agagagGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACg                               >  1:2900369/1‑93 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              <  1:5420383/93‑1 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              >  1:997339/1‑93 (MQ=255)
                                                             gaggagAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTc                            >  2:3418492/1‑93 (MQ=255)
                                                             gaggagAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTc                            <  2:6504344/93‑1 (MQ=255)
                                                              aggagAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCa                           <  2:549743/93‑1 (MQ=255)
                                                              aggagAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCa                           >  2:2686640/1‑93 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         >  1:892114/1‑93 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         <  1:5610049/93‑1 (MQ=255)
                                                                  gaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataat                       >  2:1255629/1‑93 (MQ=255)
                                                                  gaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataat                       >  1:5757358/1‑93 (MQ=255)
                                                                  gaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataat                       >  2:2420348/1‑93 (MQ=255)
                                                                    aaaTTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAtaataa                     <  2:4312500/93‑1 (MQ=255)
                                                                      aTTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGa                   >  2:4710248/1‑93 (MQ=255)
                                                                       ttCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaa                  <  2:3234742/93‑1 (MQ=255)
                                                                         cgcgCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaaaa                <  1:601165/93‑1 (MQ=255)
                                                                         cgcgCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaaaa                <  2:2946556/93‑1 (MQ=255)
                                                                          gcgcAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt               <  1:2431722/93‑1 (MQ=255)
                                                                           cgcAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATc              >  1:1043037/1‑93 (MQ=255)
                                                                           cgcAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATc              <  2:5080016/93‑1 (MQ=255)
                                                                              aaTCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa           >  1:2108394/1‑93 (MQ=255)
                                                                               aTCATGGCACCTTGC‑GCTAAAGCGTGTAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc          <  2:1308415/93‑1 (MQ=255)
                                                                               aTCATGGCACCTT‑TCGCTAAGGCGTGCAAGCCAGGCCTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc          <  1:4175029/93‑1 (MQ=255)
                                                                               aTCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc          <  1:2690336/93‑1 (MQ=255)
                                                                               aTCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc          >  1:2565933/1‑93 (MQ=255)
                                                                                tCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa         >  2:2361421/1‑93 (MQ=255)
                                                                                 cATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACaa        <  2:138755/93‑1 (MQ=255)
                                                                                 cATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACaa        <  2:2467154/93‑1 (MQ=255)
                                                                                  aTGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAt       <  1:352272/93‑1 (MQ=255)
                                                                                    ggCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAc     <  2:5845704/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    <  1:5661986/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    <  2:2350003/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    <  1:3697847/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    >  2:36065/1‑93 (MQ=255)
                                                                                       aCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGc  >  2:186883/1‑93 (MQ=255)
                                                                                       aCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGGCATAATAATAAAATCAAACAATACAGc  >  2:2477010/1‑93 (MQ=255)
                                                                                              |                                                                                      
GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC  >  NC_000913/3107377‑3107555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562
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CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T                                                                                                <  SRR4302547.899952/94‑1 (MQ=60)
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T                                                                                                <  SRR4302547.6176318/94‑1 (MQ=60)
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T                                                                                                >  SRR4302547.1359923/1‑94 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑C                                                                                              >  SRR4302547.6551330/1‑94 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑Tcg                                                                                              <  SRR4302547.4537961/94‑3 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑Tcg                                                                                              <  SRR4302547.1795350/94‑3 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑Tcg                                                                                              >  SRR4302547.1218920/1‑92 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑Tcg                                                                                              <  SRR4302547.5236584/94‑3 (MQ=60)
   AACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑Tcgc                                                                                             >  SRR4302547.36764/1‑91 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCT                                                                                            >  SRR4302547.294872/1‑94 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCT                                                                                            <  SRR4302547.4245295/94‑1 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCT                                                                                            >  SRR4302547.345468/1‑94 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCT                                                                                            <  SRR4302547.6557356/94‑1 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCT                                                                                            >  SRR4302547.2489079/1‑94 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCT                                                                                            <  SRR4302547.883877/94‑1 (MQ=60)
     CGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTA                                                                                           <  SRR4302547.2018409/94‑1 (MQ=60)
     CGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTCCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTA                                                                                           >  SRR4302547.2390963/1‑94 (MQ=60)
      GGCGATAATCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAA                                                                                          <  SRR4302547.1675493/94‑1 (MQ=60)
      GGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAA                                                                                          <  SRR4302547.4251081/94‑1 (MQ=60)
       GCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATAATGGCACCTT‑TCGCTAAA                                                                                         <  SRR4302547.3233146/94‑1 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAG                                                                                        <  SRR4302547.4910199/94‑1 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAG                                                                                        <  SRR4302547.4974168/94‑1 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAG                                                                                        >  SRR4302547.1923580/1‑94 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAG                                                                                        >  SRR4302547.1970803/1‑94 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TAGCTAAAGC                                                                                       >  SRR4302547.6769177/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCG                                                                                      <  SRR4302547.262812/94‑1 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCG                                                                                      <  SRR4302547.2994229/94‑1 (MQ=60)
           TAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGT                                                                                     >  SRR4302547.5335477/1‑94 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTG                                                                                    <  SRR4302547.998753/94‑1 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTG                                                                                    >  SRR4302547.1446703/1‑94 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTG                                                                                    >  SRR4302547.1827279/1‑94 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCTCCTT‑TCGCTAAAGCGTGC                                                                                   <  SRR4302547.3364700/94‑1 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAG                                                                                >  SRR4302547.5361994/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAG                                                                                <  SRR4302547.3411874/94‑1 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAG                                                                                >  SRR4302547.2380308/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAG                                                                                >  SRR4302547.1629810/1‑94 (MQ=60)
                 AGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTCAAGCGTGCAAGC                                                                               >  SRR4302547.1800006/1‑94 (MQ=60)
                  GAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCC                                                                              <  SRR4302547.5401549/94‑1 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCA                                                                             >  SRR4302547.4115926/1‑94 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302547.1988493/94‑1 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGAC                                                                         <  SRR4302547.1758379/94‑1 (MQ=60)
                         TAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTT                                                                       <  SRR4302547.2994105/94‑1 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      <  SRR4302547.4461287/94‑1 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     >  SRR4302547.911975/1‑94 (MQ=60)
                            GGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    >  SRR4302547.4231147/1‑94 (MQ=60)
                            GGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    <  SRR4302547.1328307/94‑1 (MQ=60)
                              AGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGA                                                                  >  SRR4302547.5546839/1‑94 (MQ=60)
                               GATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAA                                                                 <  SRR4302547.6783613/94‑1 (MQ=60)
                               GATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAA                                                                 <  SRR4302547.2243269/94‑1 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                >  SRR4302547.1077393/1‑94 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                <  SRR4302547.4078532/94‑1 (MQ=60)
                                 TGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACA                                                               >  SRR4302547.5025826/1‑94 (MQ=60)
                                    TGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAAC                                                            >  SRR4302547.5113905/1‑94 (MQ=60)
                                    CGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAAC                                                            >  SRR4302547.514013/1‑94 (MQ=60)
                                     GTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACA                                                           <  SRR4302547.6589445/94‑1 (MQ=60)
                                      TGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAA                                                          >  SRR4302547.5045105/1‑94 (MQ=60)
                                        CATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGC                                                        >  SRR4302547.1522705/1‑94 (MQ=60)
                                           GGGGCAACCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCCCCTTCC‑GCTAAAGCGTGCAAGCCAGGCCTTTGCGAACAAACAAGCCAT                                                     <  SRR4302547.5032840/94‑1 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    <  SRR4302547.6425922/94‑1 (MQ=60)
                                              GCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGA                                                  >  SRR4302547.5585727/1‑94 (MQ=60)
                                               CAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAC                                                 <  SRR4302547.4540446/94‑1 (MQ=60)
                                               CAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAC                                                 <  SRR4302547.3905171/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302547.2464025/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302547.1285942/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                >  SRR4302547.6351168/1‑94 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               >  SRR4302547.6244932/1‑94 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               >  SRR4302547.959807/1‑94 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               >  SRR4302547.4562607/1‑94 (MQ=60)
                                                  ACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAAAAACAAGCAATCGACGGC                                              >  SRR4302547.889521/1‑94 (MQ=60)
                                                    TCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAC                                            >  SRR4302547.3967230/1‑94 (MQ=60)
                                                     CCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACC                                           <  SRR4302547.1274938/94‑1 (MQ=60)
                                                     CCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAGCGACGGCACC                                           <  SRR4302547.1116132/94‑1 (MQ=60)
                                                     CCGTACAGAGAGGAGAAAATACGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACC                                           >  SRR4302547.6600744/1‑94 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGACAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          >  SRR4302547.915804/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGCCTTTGCGAACAAACAAGCCATCGACGGCACCAAA                                        >  SRR4302547.1303184/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAA                                        >  SRR4302547.4810886/1‑94 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGTCATCGACGGCACCACAC                                       >  SRR4302547.409508/1‑94 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302547.22876/94‑1 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302547.6412745/94‑1 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302547.2753500/94‑1 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       >  SRR4302547.3874292/1‑94 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302547.6064559/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      >  SRR4302547.2958204/1‑94 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302547.5264064/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302547.2800547/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302547.782866/94‑1 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     >  SRR4302547.3641141/1‑94 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     >  SRR4302547.1016786/1‑94 (MQ=60)
                                                            GAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGT                                    <  SRR4302547.5532493/94‑1 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   <  SRR4302547.6316864/94‑1 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                <  SRR4302547.898471/94‑1 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                >  SRR4302547.909267/1‑94 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                >  SRR4302547.1285890/1‑94 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               >  SRR4302547.6015937/1‑94 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302547.5725812/94‑1 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              >  SRR4302547.5876402/1‑94 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              >  SRR4302547.5742437/1‑94 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              <  SRR4302547.6726582/94‑1 (MQ=60)
                                                                   GAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATA                             <  SRR4302547.3978525/94‑1 (MQ=60)
                                                                    AAAATTCGCGCAATCGTGCCCCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTGGCGACCAAACAAGCCATCGACGGCACCAAACGGTCATAATAA                            <  SRR4302547.777478/94‑1 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302547.3812527/94‑1 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         <  SRR4302547.4346824/94‑1 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         >  SRR4302547.5771755/1‑94 (MQ=60)
                                                                        TTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAA                        <  SRR4302547.2617220/94‑1 (MQ=60)
                                                                         TCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAA                       <  SRR4302547.1866589/94‑1 (MQ=60)
                                                                         TCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGAACCAAACGGTCATAATAAGAAAA                       >  SRR4302547.2692878/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      >  SRR4302547.2912578/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      <  SRR4302547.612292/94‑1 (MQ=60)
                                                                          CGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      <  SRR4302547.5813315/94‑1 (MQ=60)
                                                                           GCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATC                     >  SRR4302547.2325767/1‑94 (MQ=60)
                                                                           GCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATC                     <  SRR4302547.2480916/94‑1 (MQ=60)
                                                                           GCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATC                     >  SRR4302547.1063623/1‑94 (MQ=60)
                                                                           GCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATC                     <  SRR4302547.1789976/94‑1 (MQ=60)
                                                                            CGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCA                    >  SRR4302547.117419/1‑94 (MQ=60)
                                                                             GCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAA                   <  SRR4302547.81851/94‑1 (MQ=60)
                                                                              CAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  >  SRR4302547.2150392/1‑94 (MQ=60)
                                                                              CAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  >  SRR4302547.4528304/1‑94 (MQ=60)
                                                                              CAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  >  SRR4302547.1566481/1‑94 (MQ=60)
                                                                               AATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGGCTTTGCGAACAAACAAGCCATCGACGGCAGCAAACGGTCATAATAAGAAAggcaaag                 >  SRR4302547.4977619/1‑87 (MQ=60)
                                                                               AATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 >  SRR4302547.5648909/1‑94 (MQ=60)
                                                                               AATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 <  SRR4302547.1373200/94‑1 (MQ=60)
                                                                               AATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 >  SRR4302547.2617764/1‑94 (MQ=60)
                                                                               AATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAAAATAAGAAAATCAAAA                 >  SRR4302547.5648908/1‑94 (MQ=60)
                                                                                ATCATGGCACCTT‑TCGCTAAGGCGTGCAAGCCAGGCCTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302547.4260084/94‑1 (MQ=60)
                                                                                ATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302547.4327776/94‑1 (MQ=60)
                                                                                ATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302547.2744343/94‑1 (MQ=60)
                                                                                ATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302547.2130406/94‑1 (MQ=60)
                                                                                ATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302547.3663936/94‑1 (MQ=60)
                                                                                 TCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302547.5278880/94‑1 (MQ=60)
                                                                                 TCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACAGCACCAAACGGTCATAATAAGAAAATCAAACAA               >  SRR4302547.5207773/1‑94 (MQ=60)
                                                                                   ATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATA             <  SRR4302547.358819/94‑1 (MQ=60)
                                                                                   ATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATA             <  SRR4302547.3434776/94‑1 (MQ=60)
                                                                                    TGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            >  SRR4302547.2405657/1‑94 (MQ=60)
                                                                                     GGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           >  SRR4302547.2207421/1‑94 (MQ=60)
                                                                                     GGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           <  SRR4302547.1637328/94‑1 (MQ=60)
                                                                                      GCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302547.3773319/94‑1 (MQ=60)
                                                                                      GCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302547.5778867/94‑1 (MQ=60)
                                                                                       CACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         >  SRR4302547.1608822/1‑94 (MQ=60)
                                                                                       CACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         <  SRR4302547.374157/94‑1 (MQ=60)
                                                                                         CCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       <  SRR4302547.2119351/94‑1 (MQ=60)
                                                                                          CTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA      >  SRR4302547.753939/1‑94 (MQ=60)
                                                                                          CTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA      <  SRR4302547.5570855/94‑1 (MQ=60)
                                                                                           TT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     >  SRR4302547.4372089/1‑94 (MQ=60)
                                                                                           TT‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACA    <  SRR4302547.6481449/94‑1 (MQ=60)
                                                                                           TT‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACA    >  SRR4302547.6246574/1‑94 (MQ=60)
                                                                                            T‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAG   <  SRR4302547.6222182/94‑1 (MQ=60)
                                                                                              C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302547.5081844/1‑94 (MQ=60)
                                                                                              C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302547.3813986/1‑94 (MQ=60)
                                                                                              |                                                                                              
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑