Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,185,776 T→C F121L (TTC→CTC)  yqiG → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,185,7760TC100.0% 918.1 / NA 249F121L (TTC→CTC) yqiGputative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (121/128);  total (121/128)

Only 100 of 257 total aligned reads displayed.

GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG  >  NC_000913/3185686‑3185865
                                                                                          |                                                                                         
ggATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTAtctc                                                                                         >  2:5172222/1‑93 (MQ=255)
 gATTTACTGCTGAATTTATTGAAAAACCTCAGCTAACCCTGCTCGATGGTTGTTACCCTATCGAAAGGGAACAACAAATTCCAACTTATCTCg                                                                                        <  1:5476155/93‑1 (MQ=255)
 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        <  1:664045/93‑1 (MQ=255)
 gATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCg                                                                                        <  1:2134969/93‑1 (MQ=255)
   tttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAt                                                                                      >  2:5193842/1‑93 (MQ=255)
   tttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAt                                                                                      <  1:4326114/93‑1 (MQ=255)
    ttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATa                                                                                     >  1:569291/1‑93 (MQ=255)
    ttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATa                                                                                     <  2:317680/93‑1 (MQ=255)
    ttACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATa                                                                                     >  2:4865361/1‑93 (MQ=255)
     tACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACCAATTACAACTTATCTCGATaa                                                                                    >  1:225584/1‑93 (MQ=255)
      tttgaggaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGATAAAGAAAAACAAACTACAATTTATCTCGATaaa                                                                                   >  2:5478241/3‑93 (MQ=255)
       cTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAg                                                                                  <  2:5639596/93‑1 (MQ=255)
       cTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAg                                                                                  <  1:2356251/93‑1 (MQ=255)
        tgatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAgg                                                                                 <  1:4646442/93‑1 (MQ=255)
         gatgaATTTATTGAAAAAACTTAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:4014398/1‑93 (MQ=255)
         gatgaATTTATTGAAAAAACTCAGCAAAACCTGGTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:4940014/1‑93 (MQ=255)
         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:245303/1‑93 (MQ=255)
         gatgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAggg                                                                                >  1:5613514/1‑93 (MQ=255)
          atgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAAGTACAACTTATCTCGATAAAGGGa                                                                               >  1:3487493/1‑93 (MQ=255)
           tgaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaa                                                                              <  2:495862/93‑1 (MQ=255)
            gaATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaa                                                                             <  1:601325/93‑1 (MQ=255)
             aaTTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaaa                                                                            >  2:815312/1‑93 (MQ=255)
              aTTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGaaaag                                                                           >  1:4061040/1‑92 (MQ=255)
              aTTTATTGAAAAAACTCAGCAAAACCTGATCGATGATTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAAt                                                                           >  1:6084330/1‑93 (MQ=255)
               tttATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATg                                                                          >  1:5436660/1‑93 (MQ=255)
                 tATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAGAATAAAAACAAATTACAACTTATCTCGATTAAGGGAAAATGCa                                                                        >  1:4232733/1‑93 (MQ=255)
                 tATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCa                                                                        >  1:4853065/1‑93 (MQ=255)
                 tATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCa                                                                        >  1:2675491/1‑93 (MQ=255)
                   ttGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAAt                                                                      >  2:895956/1‑93 (MQ=255)
                     gAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTa                                                                    <  2:2289459/93‑1 (MQ=255)
                     gAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTa                                                                    <  2:398186/93‑1 (MQ=255)
                      aaaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAt                                                                   >  2:6036638/1‑93 (MQ=255)
                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  <  2:1239034/93‑1 (MQ=255)
                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  <  1:1550652/93‑1 (MQ=255)
                       aaaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATc                                                                  <  1:4600081/93‑1 (MQ=255)
                        aaaaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATcc                                                                 >  1:4199666/1‑93 (MQ=255)
                          aaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCat                                                               >  1:2946028/1‑93 (MQ=255)
                          aaCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCat                                                               >  2:3514612/1‑93 (MQ=255)
                           aCTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCata                                                              <  1:1682423/93‑1 (MQ=255)
                            cTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatat                                                             >  2:459844/1‑93 (MQ=255)
                             tCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCatatc                                                            >  2:375674/1‑92 (MQ=255)
                               aGCAAAACCTGATCGATGGTTGTGACCCTATCGAAAAAAAAAAACAAATTACAACTTATCTCGATAAATGTAAAATGGAATTATCCATATCTg                                                          >  1:1910575/1‑93 (MQ=255)
                                 cAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCa                                                        <  2:1096480/93‑1 (MQ=255)
                                  aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                       <  2:5501010/93‑1 (MQ=255)
                                  aaaaCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCAc                                                       >  2:3903622/1‑93 (MQ=255)
                                     aCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTc                                                    >  1:5528466/1‑93 (MQ=255)
                                     aCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTc                                                    <  2:6137659/93‑1 (MQ=255)
                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  2:1753033/93‑1 (MQ=255)
                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   >  2:680944/1‑93 (MQ=255)
                                      ccTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCa                                                   <  2:5063920/93‑1 (MQ=255)
                                       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                  <  2:5699707/93‑1 (MQ=255)
                                       cTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAg                                                  >  1:1822948/1‑93 (MQ=255)
                                        tGATCGATGGTTGTTACCATATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAgg                                                 <  1:3335153/93‑1 (MQ=255)
                                          aTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCa                                               <  1:2151545/93‑1 (MQ=255)
                                             gATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATgg                                            <  2:594836/93‑1 (MQ=255)
                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACATCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  2:1598520/93‑1 (MQ=255)
                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  1:2650019/93‑1 (MQ=255)
                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  1:3023856/93‑1 (MQ=255)
                                                   tgttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTaaaa                                      <  2:2015498/93‑1 (MQ=255)
                                                    gttACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAt                                     >  1:1975430/1‑93 (MQ=255)
                                                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                  <  1:1464425/93‑1 (MQ=255)
                                                       aCCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACa                                  >  1:3673828/1‑93 (MQ=255)
                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                >  2:4061315/1‑93 (MQ=255)
                                                         ccTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACaaa                                >  2:3457678/1‑93 (MQ=255)
                                                           tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                              <  2:2552899/93‑1 (MQ=255)
                                                           tATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGa                              <  2:3377190/93‑1 (MQ=255)
                                                            aTCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAt                             <  2:2802306/93‑1 (MQ=255)
                                                             tCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATg                            >  1:4490127/1‑93 (MQ=255)
                                                              cGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                           >  2:2460078/1‑93 (MQ=255)
                                                              cGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGc                           >  2:5872437/1‑93 (MQ=255)
                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  1:1135979/1‑93 (MQ=255)
                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          >  2:578506/1‑93 (MQ=255)
                                                               gaaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCa                          <  2:4661516/93‑1 (MQ=255)
                                                                aaaaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCaa                         <  1:4957344/93‑1 (MQ=255)
                                                                  aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                       >  2:1471576/1‑93 (MQ=255)
                                                                  aaagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAc                       >  1:3645349/1‑93 (MQ=255)
                                                                   aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACt                      >  2:366490/1‑93 (MQ=255)
                                                                   aagaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTGAAAATACAAAGATGCAAACt                      <  1:5933469/93‑1 (MQ=255)
                                                                    agaaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTg                     >  2:3011373/1‑93 (MQ=255)
                                                                      aaaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGa                   <  1:1749893/93‑1 (MQ=255)
                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  2:4099982/1‑93 (MQ=255)
                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:1530459/1‑93 (MQ=255)
                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:4720366/1‑93 (MQ=255)
                                                                       aaaaCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAc                  >  1:4357864/1‑93 (MQ=255)
                                                                          aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc               <  2:335113/93‑1 (MQ=255)
                                                                          aCAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcc               >  1:3742557/1‑93 (MQ=255)
                                                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct              <  2:4230725/93‑1 (MQ=255)
                                                                           cAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcct              >  1:1644047/1‑93 (MQ=255)
                                                                            aaaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctc             <  1:1000044/93‑1 (MQ=255)
                                                                             aaTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcc            >  1:3202839/1‑93 (MQ=255)
                                                                              aTTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGcctcct           <  1:5650614/93‑1 (MQ=255)
                                                                               ttACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTg          <  1:5785758/93‑1 (MQ=255)
                                                                                 aCAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGaa        >  2:747769/1‑93 (MQ=255)
                                                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt      >  2:5257925/1‑93 (MQ=255)
                                                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt      >  1:3163549/1‑93 (MQ=255)
                                                                                   aaCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACt      >  2:4001183/1‑93 (MQ=255)
                                                                                    aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt     >  1:2827854/1‑93 (MQ=255)
                                                                                    aCTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACtt     >  1:5306394/1‑93 (MQ=255)
                                                                                     cTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACttt    <  1:1348759/93‑1 (MQ=255)
                                                                                       tATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTgg  >  1:2460755/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG  >  NC_000913/3185686‑3185865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATC  >  NC_000913/3185684‑3185869
                                                                                            |                                                                                             
TAGGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCT                                                                                              <  SRR4302532.1487669/94‑1 (MQ=60)
  GGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCG                                                                                            <  SRR4302532.247304/94‑1 (MQ=60)
   GATTTACTGCTGAATTTATTGAAAAACCTCAGCTAACCCTGCTCGATGGTTGTTACCCTATCGAAAGGGAACAACAAATTCCAACTTATCTCGA                                                                                           <  SRR4302532.5666035/94‑1 (MQ=42)
   GATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGA                                                                                           <  SRR4302532.686999/94‑1 (MQ=60)
   GATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGA                                                                                           <  SRR4302532.2206279/94‑1 (MQ=60)
    ATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAT                                                                                          >  SRR4302532.4343212/1‑94 (MQ=60)
    ATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGAT                                                                                          <  SRR4302532.5919309/94‑1 (MQ=60)
     TTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATA                                                                                         <  SRR4302532.4472285/94‑1 (MQ=60)
     TTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATA                                                                                         >  SRR4302532.589157/1‑94 (MQ=60)
      TTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACCAATTACAACTTATCTCGATAA                                                                                        >  SRR4302532.233289/1‑94 (MQ=60)
      TTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAA                                                                                        >  SRR4302532.233292/1‑94 (MQ=60)
      TTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAA                                                                                        >  SRR4302532.233291/1‑94 (MQ=60)
       TGCTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAA                                                                                       <  SRR4302532.4443674/94‑1 (MQ=60)
       TACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAA                                                                                       <  SRR4302532.5067592/94‑1 (MQ=60)
       TACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAA                                                                                       >  SRR4302532.4826677/1‑94 (MQ=60)
         CTGATGAATTTATTGAAAAAACTCAGCAAACCCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTAGAACTTATCTCGATAAAGG                                                                                     <  SRR4302532.3683287/94‑1 (MQ=60)
         CTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGG                                                                                     >  SRR4302532.4624553/1‑94 (MQ=60)
         CTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGG                                                                                     <  SRR4302532.2434863/94‑1 (MQ=60)
          TGATGAATTTATTGAAAAAACTTAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    >  SRR4302532.4149663/1‑94 (MQ=60)
          TGATGAATTTATTGAAAAAACTCAGCAAAACCTGGTCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    >  SRR4302532.5109581/1‑94 (MQ=60)
          TGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    <  SRR4302532.1745885/94‑1 (MQ=60)
          TGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    <  SRR4302532.619066/94‑1 (MQ=60)
          TGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    >  SRR4302532.5808301/1‑94 (MQ=60)
          TGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    <  SRR4302532.4804856/94‑1 (MQ=60)
          TGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGG                                                                                    >  SRR4302532.253804/1‑94 (MQ=60)
           GATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGA                                                                                   >  SRR4302532.3394785/1‑94 (MQ=60)
           GATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGA                                                                                   <  SRR4302532.3270640/94‑1 (MQ=60)
           GATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAAGTACAACTTATCTCGATAAAGGGA                                                                                   >  SRR4302532.3604340/1‑94 (MQ=60)
            ATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAA                                                                                  <  SRR4302532.2977036/94‑1 (MQ=60)
             TGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAA                                                                                 >  SRR4302532.3392976/1‑94 (MQ=60)
              GAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAA                                                                                <  SRR4302532.622328/94‑1 (MQ=60)
               AATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAAT                                                                               <  SRR4302532.4524476/94‑1 (MQ=60)
               AATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAAT                                                                               <  SRR4302532.5112237/94‑1 (MQ=60)
               AATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAAG                                                                               >  SRR4302532.4197530/1‑94 (MQ=60)
               AATTTATTGAAAAAACTCAGCAAAACCTGATCGATGATTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAAT                                                                               >  SRR4302532.6294426/1‑94 (MQ=60)
                ATTTATTGAAAAAACTCAGCAAACCTTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAATGTAAAATG                                                                              <  SRR4302532.3851686/94‑1 (MQ=60)
                ATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATG                                                                              <  SRR4302532.1820491/94‑1 (MQ=60)
                ATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATG                                                                              >  SRR4302532.5625075/1‑94 (MQ=60)
                ATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATG                                                                              >  SRR4302532.2822743/1‑94 (MQ=60)
                  TTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAGAATAAAAACAAATTACAACTTATCTCGATTAAGGGAAAATGCA                                                                            >  SRR4302532.4374841/1‑94 (MQ=60)
                  TTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCA                                                                            >  SRR4302532.2765292/1‑94 (MQ=60)
                  TTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCA                                                                            >  SRR4302532.5020060/1‑94 (MQ=60)
                  TTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCA                                                                            >  SRR4302532.4474344/1‑94 (MQ=60)
                  TCATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCA                                                                            >  SRR4302532.3771647/1‑94 (MQ=60)
                   TATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAA                                                                           <  SRR4302532.6305304/94‑1 (MQ=60)
                   TATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAA                                                                           >  SRR4302532.1724001/1‑94 (MQ=60)
                     TTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATT                                                                         <  SRR4302532.5315746/94‑1 (MQ=60)
                     TTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATT                                                                         <  SRR4302532.1954152/94‑1 (MQ=60)
                       GAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAT                                                                       >  SRR4302532.6269976/1‑94 (MQ=60)
                       GAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAT                                                                       >  SRR4302532.4190626/1‑94 (MQ=60)
                       GAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAT                                                                       <  SRR4302532.4267165/94‑1 (MQ=60)
                       GAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTAT                                                                       <  SRR4302532.305062/94‑1 (MQ=60)
                         AAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCC                                                                     <  SRR4302532.5792707/94‑1 (MQ=60)
                         AAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCC                                                                     >  SRR4302532.4340616/1‑94 (MQ=60)
                         AAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCC                                                                     <  SRR4302532.1603069/94‑1 (MQ=60)
                         AAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCC                                                                     <  SRR4302532.4756915/94‑1 (MQ=60)
                         AAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCA                                                                     >  SRR4302532.1781194/1‑94 (MQ=60)
                           AAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCAT                                                                   >  SRR4302532.3043768/1‑94 (MQ=60)
                           AAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCAT                                                                   <  SRR4302532.1513797/94‑1 (MQ=60)
                             ACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATAT                                                                 <  SRR4302532.1739282/94‑1 (MQ=60)
                             ACCCAACAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATAT                                                                 <  SRR4302532.2244280/94‑1 (MQ=60)
                              CTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATC                                                                >  SRR4302532.5224918/1‑94 (MQ=60)
                                CAGCAAAACCTGATCGATGGTTGTGACCCTATCGAAAAAAAAAAACAAATTACAACTTATCTCGATAAATGTAAAATGGAATTATCCATATCTG                                                              >  SRR4302532.1974030/1‑94 (MQ=60)
                                  GCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCA                                                            >  SRR4302532.395593/1‑94 (MQ=60)
                                  GCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCA                                                            <  SRR4302532.1545745/94‑1 (MQ=60)
                                    AAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACC                                                          >  SRR4302532.5697897/1‑94 (MQ=60)
                                      AACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTC                                                        >  SRR4302532.5720323/1‑94 (MQ=60)
                                      AACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTC                                                        >  SRR4302532.5569697/1‑94 (MQ=60)
                                       ACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCA                                                       <  SRR4302532.4981410/94‑1 (MQ=60)
                                        CCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAG                                                      >  SRR4302532.1883666/1‑94 (MQ=60)
                                        CCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAG                                                      <  SRR4302532.183105/94‑1 (MQ=60)
                                          TGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGC                                                    <  SRR4302532.6274261/94‑1 (MQ=60)
                                          TGATCGATGGTTGTTACCATATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGC                                                    <  SRR4302532.3446414/94‑1 (MQ=60)
                                            ATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCAT                                                  <  SRR4302532.3205554/94‑1 (MQ=60)
                                            ATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCAT                                                  <  SRR4302532.2223438/94‑1 (MQ=60)
                                            ATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCAT                                                  <  SRR4302532.2223437/94‑1 (MQ=60)
                                              CGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGG                                                <  SRR4302532.5822966/94‑1 (MQ=60)
                                              CGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGG                                                >  SRR4302532.6098663/1‑94 (MQ=60)
                                              CGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGG                                                >  SRR4302532.2529488/1‑94 (MQ=60)
                                                ATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTT                                              >  SRR4302532.1276858/1‑94 (MQ=60)
                                                ATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTT                                              <  SRR4302532.4648049/94‑1 (MQ=60)
                                                  GGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAA                                            >  SRR4302532.961423/1‑94 (MQ=60)
                                                     TGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAT                                         >  SRR4302532.2041045/1‑94 (MQ=60)
                                                     TGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAT                                         <  SRR4302532.2535373/94‑1 (MQ=60)
                                                     TGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAT                                         <  SRR4302532.2738920/94‑1 (MQ=60)
                                                     TGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAAT                                         <  SRR4302532.3123600/94‑1 (MQ=60)
                                                      GTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATA                                        >  SRR4302532.2895345/1‑94 (MQ=60)
                                                      GTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATA                                        >  SRR4302532.3504534/1‑94 (MQ=60)
                                                       TTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATAC                                       >  SRR4302532.4139709/1‑94 (MQ=60)
                                                       TTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATAC                                       <  SRR4302532.1587234/94‑1 (MQ=60)
                                                       TTACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATAC                                       >  SRR4302532.1412691/1‑94 (MQ=60)
                                                        TACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACA                                      >  SRR4302532.3797235/1‑94 (MQ=60)
                                                         ACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAA                                     <  SRR4302532.6093344/94‑1 (MQ=60)
                                                         ACCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAA                                     <  SRR4302532.1513767/94‑1 (MQ=60)
                                                          CCCTATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAA                                    >  SRR4302532.5855516/1‑94 (MQ=60)
                                                             TATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAT                                 >  SRR4302532.483944/1‑94 (MQ=60)
                                                             TATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGAT                                 <  SRR4302532.4236922/94‑1 (MQ=60)
                                                              ATCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATG                                >  SRR4302532.4643395/1‑94 (MQ=60)
                                                               TCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGC                               >  SRR4302532.2755880/1‑94 (MQ=60)
                                                               TCGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGC                               <  SRR4302532.5840267/94‑1 (MQ=60)
                                                                CGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCA                              >  SRR4302532.1173872/1‑94 (MQ=60)
                                                                CGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCA                              >  SRR4302532.3854089/1‑94 (MQ=60)
                                                                CGAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCA                              >  SRR4302532.1067629/1‑94 (MQ=60)
                                                                 GAAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAA                             >  SRR4302532.5631310/1‑94 (MQ=60)
                                                                  AAAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAA                            <  SRR4302532.5127393/94‑1 (MQ=60)
                                                                   AAAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAAC                           >  SRR4302532.3767665/1‑94 (MQ=60)
                                                                    AAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACT                          <  SRR4302532.3237555/94‑1 (MQ=60)
                                                                    AAAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACT                          >  SRR4302532.5921592/1‑94 (MQ=60)
                                                                     AAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTG                         >  SRR4302532.1678595/1‑94 (MQ=60)
                                                                     AAGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTGAAAATACAAAGATGCAAACTG                         <  SRR4302532.6138840/94‑1 (MQ=60)
                                                                      AGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGG                        <  SRR4302532.4762204/94‑1 (MQ=60)
                                                                      AGAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGG                        <  SRR4302532.2219369/94‑1 (MQ=60)
                                                                       GAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGA                       >  SRR4302532.5550001/1‑94 (MQ=60)
                                                                       GAAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGA                       >  SRR4302532.1984924/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.2545902/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      <  SRR4302532.3670906/94‑1 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.2239304/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.4439758/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.5055222/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.4881423/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.4505474/1‑94 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      <  SRR4302532.1808698/94‑1 (MQ=60)
                                                                        AAAAACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGAC                      >  SRR4302532.1582163/1‑94 (MQ=60)
                                                                           AACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCC                   >  SRR4302532.3868339/1‑94 (MQ=60)
                                                                           AACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCC                   >  SRR4302532.2693578/1‑94 (MQ=60)
                                                                            ACAAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCT                  >  SRR4302532.1699734/1‑94 (MQ=60)
                                                                              AAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCC                <  SRR4302532.1034097/94‑1 (MQ=60)
                                                                              AAATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCC                >  SRR4302532.3309182/1‑94 (MQ=60)
                                                                               AATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCT               <  SRR4302532.2089658/94‑1 (MQ=60)
                                                                                ATTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTG              <  SRR4302532.5847241/94‑1 (MQ=60)
                                                                                 TTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGA             <  SRR4302532.1697408/94‑1 (MQ=60)
                                                                                 TTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGA             <  SRR4302532.5986963/94‑1 (MQ=60)
                                                                                 TTACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGA             <  SRR4302532.4725340/94‑1 (MQ=60)
                                                                                  TACAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAA            <  SRR4302532.5694830/94‑1 (MQ=60)
                                                                                    CAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACT          >  SRR4302532.1997215/1‑94 (MQ=60)
                                                                                    CAACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACT          >  SRR4302532.3268603/1‑94 (MQ=60)
                                                                                     AACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTT         >  SRR4302532.2922438/1‑94 (MQ=60)
                                                                                     AACTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTT         >  SRR4302532.5489859/1‑94 (MQ=60)
                                                                                       CTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTG       <  SRR4302532.3101151/94‑1 (MQ=60)
                                                                                       CTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTG       <  SRR4302532.1393856/94‑1 (MQ=60)
                                                                                       CTTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTG       <  SRR4302532.1393855/94‑1 (MQ=60)
                                                                                        TTATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGG      >  SRR4302532.2542630/1‑94 (MQ=60)
                                                                                          ATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAA    >  SRR4302532.610209/1‑94 (MQ=60)
                                                                                          ATCTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAA    >  SRR4302532.3434332/1‑94 (MQ=60)
                                                                                            CTCGATAAAGGGAAAATGCAATTATCCATATCTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATC  >  SRR4302532.3570063/1‑94 (MQ=60)
                                                                                            |                                                                                             
TAGGATTTACTGATGAATTTATTGAAAAAACTCAGCAAAACCTGATCGATGGTTGTTACCCTATCGAAAAAGAAAAACAAATTACAACTTATTTCGATAAAGGGAAAATGCAATTATCCATATTTGCACCTCAGGCATGGTTAAAATACAAAGATGCAAACTGGACGCCTCCTGAACTTTGGAATC  >  NC_000913/3185684‑3185869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑