Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,926 +C coding (1052/1434 nt) ygjI → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,9241.C99.5% 747.7 / ‑5.2 208Y350Y (TAT→TAC) ygjIputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  major base C (84/121);  minor base G (1/0);  total (86/122)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 241 total aligned reads displayed.

CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224831‑3225011
                                                                                              |                                                                                       
 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                        >  1:1633094/1‑93 (MQ=255)
 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                        <  2:3986727/93‑1 (MQ=255)
 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                        <  1:1760518/93‑1 (MQ=255)
 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                        >  1:5791897/1‑93 (MQ=255)
  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                                       <  2:5540462/93‑1 (MQ=255)
  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                                       <  2:5996506/93‑1 (MQ=255)
  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                                       >  1:1919947/1‑93 (MQ=255)
   tGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccc                                                                                      <  2:1793339/93‑2 (MQ=255)
    ggTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccg                                                                                     >  2:2373497/1‑91 (MQ=255)
     gTAAGAAAGCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                                    <  1:1988406/93‑4 (MQ=255)
     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                                    <  1:2125678/93‑4 (MQ=255)
      tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                                                                   >  2:964914/1‑89 (MQ=255)
      tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                                                                   >  1:1499998/1‑89 (MQ=255)
         gAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCt                                                                                 >  1:2870799/1‑93 (MQ=255)
          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                                                <  1:3350037/93‑1 (MQ=255)
          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                                                <  1:4257938/93‑1 (MQ=255)
          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                                                <  2:268803/93‑1 (MQ=255)
            aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCgg                                                                              <  1:481152/93‑1 (MQ=255)
               cGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGGTCGGTTc                                                                           <  2:2145063/93‑1 (MQ=255)
               cGTCGAACTGAACGAAAACAGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTc                                                                           >  1:2542700/1‑93 (MQ=255)
                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                                                                          >  1:6116823/1‑93 (MQ=255)
                gTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTcc                                                                          >  2:7869/1‑93 (MQ=255)
                 tCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCa                                                                         >  1:5011459/1‑93 (MQ=255)
                  cGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCaa                                                                        <  1:547836/93‑1 (MQ=255)
                   gAACTGAACGAAAACGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAAt                                                                       <  2:4485908/93‑1 (MQ=255)
                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                                      <  2:2251624/93‑1 (MQ=255)
                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                                      >  1:2223962/1‑93 (MQ=255)
                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                                     >  2:3319381/1‑93 (MQ=255)
                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                                     >  2:1953981/1‑93 (MQ=255)
                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                                     <  1:2356421/93‑1 (MQ=255)
                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                                                                    <  2:5483090/93‑1 (MQ=255)
                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                                                                    >  2:4722328/1‑93 (MQ=255)
                      atGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                                                                    <  1:116416/92‑1 (MQ=255)
                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGt                                                                  <  2:16485/93‑1 (MQ=255)
                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGt                                                                  <  2:4112418/93‑1 (MQ=255)
                         aaCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                                 >  2:5608159/1‑93 (MQ=255)
                         aaCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTg                                                                 >  1:1666151/1‑93 (MQ=255)
                          aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGc                                                                <  1:1054692/93‑1 (MQ=255)
                           cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                                               >  2:1323800/1‑93 (MQ=255)
                           cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATACTCGGTTCCAATACCGTGCa                                                               <  1:4766652/93‑1 (MQ=255)
                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAg                                                              <  2:1111781/93‑1 (MQ=255)
                              agaCGGCGTTCTGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGa                                                            <  1:4664279/91‑1 (MQ=255)
                              aaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGa                                                            <  1:384274/93‑1 (MQ=255)
                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                           >  2:2837348/1‑93 (MQ=255)
                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc                                                          <  2:2480732/93‑1 (MQ=255)
                                 cGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCt                                                         >  1:5060322/1‑93 (MQ=255)
                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                       >  2:3462940/1‑93 (MQ=255)
                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                       <  2:581500/93‑1 (MQ=255)
                                    cGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                                                      <  1:6035582/93‑1 (MQ=255)
                                    cGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgat                                                      <  1:1433903/93‑1 (MQ=255)
                                     cttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                     <  2:5047676/92‑1 (MQ=255)
                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                    >  2:3733882/1‑93 (MQ=255)
                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatga                                                    >  1:1122904/1‑93 (MQ=255)
                                       tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                                                   <  2:1246432/93‑1 (MQ=255)
                                         cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                 <  1:696207/93‑1 (MQ=255)
                                         cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                 >  1:3678827/1‑93 (MQ=255)
                                            gcgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACt                                               <  2:5914699/89‑1 (MQ=255)
                                               cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                            <  2:4623646/92‑1 (MQ=255)
                                                gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                           >  1:3038403/1‑93 (MQ=255)
                                                 cgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtattat                                          <  1:3138742/93‑1 (MQ=255)
                                                  gcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                                         <  2:4974046/93‑1 (MQ=255)
                                                    aGCGTGGATCCAGTTCCTTATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       <  2:2031720/93‑1 (MQ=255)
                                                    aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       <  2:4227132/93‑1 (MQ=255)
                                                    aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       >  1:1099735/1‑93 (MQ=255)
                                                    aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       >  1:846760/1‑93 (MQ=255)
                                                     gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      <  1:1805982/93‑1 (MQ=255)
                                                     gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      <  1:3101423/93‑1 (MQ=255)
                                                     gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      <  1:3276645/93‑1 (MQ=255)
                                                      cGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                     <  2:682977/93‑1 (MQ=255)
                                                      cGTGGATCCAGTTCCTCATCGTCATCCCGCTGATGATTATCCCGATCCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAata                                     <  1:4409719/93‑1 (MQ=255)
                                                       gtagATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  1:3553447/90‑1 (MQ=255)
                                                       gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    >  1:2449302/1‑93 (MQ=255)
                                                       gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  1:5381398/93‑1 (MQ=255)
                                                        tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                                   <  2:326315/93‑1 (MQ=255)
                                                        tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                                   <  2:4252079/93‑1 (MQ=255)
                                                        tGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATg                                   <  2:532528/93‑1 (MQ=255)
                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                  <  2:4834312/93‑1 (MQ=255)
                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                  >  2:4026562/1‑93 (MQ=255)
                                                            tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                               <  2:5902445/93‑1 (MQ=255)
                                                                gTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgc                           <  2:1459458/93‑1 (MQ=255)
                                                                gTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgc                           <  1:1264626/93‑1 (MQ=255)
                                                                 ttCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          <  1:2652682/93‑1 (MQ=255)
                                                                 ttCCTGATCGTCATCCCGCTGATGATTATCCCAATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          >  2:1214991/1‑93 (MQ=255)
                                                                  tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                         >  1:4832434/1‑93 (MQ=255)
                                                                  tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                         <  1:4868885/93‑1 (MQ=255)
                                                                     tGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATTACCGCCGTCGCGt                      >  1:3968725/1‑93 (MQ=255)
                                                                      gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                     <  1:3642611/93‑1 (MQ=255)
                                                                      gATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTc                     >  2:4336835/1‑93 (MQ=255)
                                                                        tCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   <  1:1575526/93‑1 (MQ=255)
                                                                        tCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   <  1:832054/93‑1 (MQ=255)
                                                                         cGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAt                  <  2:5356960/93‑1 (MQ=255)
                                                                              tCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCGTGCAGGATCTGATGACTACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             >  2:3433/1‑93 (MQ=255)
                                                                               cccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTcc            <  2:5721890/93‑1 (MQ=255)
                                                                                     gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      <  1:564387/93‑1 (MQ=255)
                                                                                     gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      >  1:2824554/1‑93 (MQ=255)
                                                                                     gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      >  2:3128255/1‑93 (MQ=255)
                                                                                     gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      >  2:704041/1‑93 (MQ=255)
                                                                                       tgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt    >  1:5712813/1‑93 (MQ=255)
                                                                                       tgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt    >  1:4331078/1‑93 (MQ=255)
                                                                                         atTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATtc  >  1:981918/1‑93 (MQ=255)
                                                                                              |                                                                                       
CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224831‑3225011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224832‑3225019
                                                                                               |                                                                                              
TTTTGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC                                                                                                <  SRR4302532.1747672/94‑1 (MQ=60)
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC                                                                                                <  SRR4302532.1819576/94‑1 (MQ=60)
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC                                                                                                >  SRR4302532.1983796/1‑94 (MQ=60)
    GTAAGAAAGCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            <  SRR4302532.2054491/94‑1 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            <  SRR4302532.2196699/94‑1 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            <  SRR4302532.5198489/94‑1 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            >  SRR4302532.1550609/1‑94 (MQ=60)
    GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            >  SRR4302532.2966971/1‑94 (MQ=60)
     TAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATG                                                                                           >  SRR4302532.4233952/1‑94 (MQ=60)
      AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGC                                                                                          >  SRR4302532.2948076/1‑94 (MQ=60)
      AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGC                                                                                          >  SRR4302532.870278/1‑94 (MQ=60)
       AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCT                                                                                         <  SRR4302532.3028349/94‑1 (MQ=60)
       AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCT                                                                                         >  SRR4302532.1018354/1‑94 (MQ=60)
       AGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCT                                                                                         >  SRR4302532.2966801/1‑94 (MQ=60)
         AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCG                                                                                       <  SRR4302532.4401176/94‑1 (MQ=60)
         AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCG                                                                                       <  SRR4302532.3461889/94‑1 (MQ=60)
           AACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGT                                                                                     <  SRR4302532.497931/94‑1 (MQ=60)
            ACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GGTGCTCGGTT                                                                                    >  SRR4302532.1579186/1‑94 (MQ=60)
             CCGTCGAACTGAACGAAAACAGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTC                                                                                   >  SRR4302532.2627509/1‑94 (MQ=60)
              CGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCC                                                                                  >  SRR4302532.6327921/1‑94 (MQ=60)
               GTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCA                                                                                 >  SRR4302532.5183234/1‑94 (MQ=60)
                 CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               >  SRR4302532.1690719/1‑94 (MQ=60)
                 CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               >  SRR4302532.3445072/1‑94 (MQ=60)
                 CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               <  SRR4302532.566935/94‑1 (MQ=60)
                  GAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATA                                                                              >  SRR4302532.2297782/1‑94 (MQ=60)
                  GAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATA                                                                              <  SRR4302532.4780119/94‑1 (MQ=60)
                    ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302532.2788558/94‑1 (MQ=60)
                    ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302532.4301258/94‑1 (MQ=60)
                    ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302532.2435037/94‑1 (MQ=60)
                    ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302532.1475512/94‑1 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           <  SRR4302532.691992/94‑1 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           >  SRR4302532.1528009/1‑94 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           <  SRR4302532.4516304/94‑1 (MQ=60)
                     CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGGGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           <  SRR4302532.120408/94‑1 (MQ=60)
                     ATGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           <  SRR4302532.120410/94‑1 (MQ=60)
                       GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         <  SRR4302532.3939328/94‑1 (MQ=60)
                       GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         >  SRR4302532.1836428/1‑94 (MQ=60)
                       GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         >  SRR4302532.1722555/1‑94 (MQ=60)
                         ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCA                                                                       <  SRR4302532.1090253/94‑1 (MQ=60)
                          CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCTTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302532.3894361/94‑1 (MQ=60)
                          CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATACTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302532.4930115/94‑1 (MQ=60)
                             AGACGGCGTTCTGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGAT                                                                   <  SRR4302532.4823297/94‑1 (MQ=60)
                             AAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGAT                                                                   <  SRR4302532.397710/94‑1 (MQ=60)
                             AAACGGCGTCCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGAT                                                                   <  SRR4302532.1006124/94‑1 (MQ=60)
                              AACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATC                                                                  <  SRR4302532.379078/94‑1 (MQ=60)
                               ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCTGTTCCAATACCGTGCAGGATCT                                                                 <  SRR4302532.6263207/94‑1 (MQ=60)
                               ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCT                                                                 >  SRR4302532.5234037/1‑94 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302532.6353148/1‑94 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302532.6244015/94‑1 (MQ=60)
                                   CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             <  SRR4302532.1482181/94‑1 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302532.3149412/1‑94 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302532.1160397/1‑94 (MQ=60)
                                    GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            >  SRR4302532.5470066/1‑94 (MQ=60)
                                     TTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA                                                           <  SRR4302532.722093/94‑1 (MQ=60)
                                     TTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA                                                           <  SRR4302532.4970508/94‑1 (MQ=60)
                                      TCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATTGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAT                                                          >  SRR4302532.5154543/1‑94 (MQ=60)
                                       CCGGCGC‑GCGCAGCGTGGATCCATTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA                                                         <  SRR4302532.2027968/94‑1 (MQ=60)
                                       CCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA                                                         >  SRR4302532.3802410/1‑94 (MQ=60)
                                       CCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA                                                         <  SRR4302532.2027967/94‑1 (MQ=60)
                                        CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        <  SRR4302532.720050/94‑1 (MQ=60)
                                        CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        <  SRR4302532.4985347/94‑1 (MQ=60)
                                         GGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT                                                       <  SRR4302532.1733577/94‑1 (MQ=60)
                                          GCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTA                                                      <  SRR4302532.2289009/94‑1 (MQ=60)
                                             gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    >  SRR4302532.2580455/3‑94 (MQ=60)
                                              CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   >  SRR4302532.3138651/1‑94 (MQ=60)
                                              CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   <  SRR4302532.5974342/94‑1 (MQ=60)
                                              CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATACC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   >  SRR4302532.2756139/1‑94 (MQ=60)
                                               GCGCAGCGTGGATCCAGTTCCTGCTCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTAT                                                  <  SRR4302532.3608547/94‑1 (MQ=60)
                                                CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302532.3242908/94‑1 (MQ=60)
                                                  CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               >  SRR4302532.875856/1‑94 (MQ=60)
                                                  CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               <  SRR4302532.4965148/94‑1 (MQ=60)
                                                  CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               >  SRR4302532.1136491/1‑94 (MQ=60)
                                                   AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302532.1941369/1‑94 (MQ=60)
                                                   AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302532.3230541/1‑94 (MQ=60)
                                                    GCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA                                             <  SRR4302532.3385615/94‑1 (MQ=60)
                                                    GCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA                                             <  SRR4302532.1866264/94‑1 (MQ=60)
                                                    GCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA                                             <  SRR4302532.3204281/94‑1 (MQ=60)
                                                     GGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAGGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302532.2116675/94‑1 (MQ=60)
                                                     CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302532.2724020/94‑1 (MQ=60)
                                                     CGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            >  SRR4302532.2530744/1‑94 (MQ=60)
                                                     CGTGGATCCAGTTCCTCATCGTCATCCCGCTGATGATTATCCC‑GATCCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATAT                                            <  SRR4302532.4559941/94‑1 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           <  SRR4302532.518257/94‑1 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           <  SRR4302532.5567795/94‑1 (MQ=60)
                                                      GTGGATCCAGTTCCTGATCGCCATCCCGCTGATGATTATGCC‑GATTCTCGGTTCCAATACCATGCAGGATCTGATGAATACTATTATTAATATA                                           >  SRR4302532.5624727/1‑94 (MQ=60)
                                                      GTAGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           <  SRR4302532.3672342/94‑1 (MQ=60)
                                                       TGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGA                                          <  SRR4302532.1246258/94‑1 (MQ=60)
                                                        GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         <  SRR4302532.1175688/94‑1 (MQ=60)
                                                        GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         <  SRR4302532.1003381/94‑1 (MQ=60)
                                                         GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302532.3719635/94‑1 (MQ=60)
                                                         GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302532.2383929/94‑1 (MQ=60)
                                                           TCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGC                                      >  SRR4302532.5755574/1‑94 (MQ=60)
                                                             CAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCG                                    >  SRR4302532.1661712/1‑94 (MQ=60)
                                                              AGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGC                                   >  SRR4302532.3334715/1‑94 (MQ=60)
                                                               GTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCA                                  <  SRR4302532.1306928/94‑1 (MQ=60)
                                                                TTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAG                                 <  SRR4302532.2741703/94‑1 (MQ=60)
                                                                TTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAG                                 >  SRR4302532.4998776/1‑94 (MQ=60)
                                                                 TCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                >  SRR4302532.5291584/1‑94 (MQ=60)
                                                                 TCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                <  SRR4302532.5036299/94‑1 (MQ=60)
                                                                   CTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATTACCGCCGTCGCGT                              >  SRR4302532.4102423/1‑94 (MQ=60)
                                                                   CTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGT                              >  SRR4302532.3999181/1‑94 (MQ=60)
                                                                    TGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTC                             >  SRR4302532.5908447/1‑94 (MQ=60)
                                                                     GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            <  SRR4302532.3764838/94‑1 (MQ=60)
                                                                      ATCGTCATCCCGCGGATGATTATCCC‑GAGGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCA                           <  SRR4302532.1120755/94‑1 (MQ=60)
                                                                       TCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          <  SRR4302532.1628866/94‑1 (MQ=60)
                                                                       TCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          <  SRR4302532.1628867/94‑1 (MQ=60)
                                                                       TCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          <  SRR4302532.860571/94‑1 (MQ=60)
                                                                       TCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          <  SRR4302532.6162799/94‑1 (MQ=60)
                                                                        CGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302532.4722202/94‑1 (MQ=60)
                                                                          TCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       >  SRR4302532.2228398/1‑94 (MQ=60)
                                                                            ATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTC                     >  SRR4302532.5698980/1‑94 (MQ=60)
                                                                              CCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCG                   >  SRR4302532.4223790/1‑94 (MQ=60)
                                                                                CGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 <  SRR4302532.2552704/94‑1 (MQ=60)
                                                                                CGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCC                 >  SRR4302532.4452897/1‑94 (MQ=60)
                                                                                  CTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGT               >  SRR4302532.1155540/1‑94 (MQ=60)
                                                                                   TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGTCGTT              >  SRR4302532.1599853/1‑94 (MQ=60)
                                                                                   TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              <  SRR4302532.1428714/94‑1 (MQ=60)
                                                                                   TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              >  SRR4302532.2919049/1‑94 (MQ=60)
                                                                                   TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              >  SRR4302532.329729/1‑94 (MQ=60)
                                                                                    GATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTA             <  SRR4302532.584074/94‑1 (MQ=60)
                                                                                     ATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAT            >  SRR4302532.5911903/1‑94 (MQ=60)
                                                                                     ATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAT            >  SRR4302532.4477399/1‑94 (MQ=60)
                                                                                     ATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAT            <  SRR4302532.4922891/94‑1 (MQ=60)
                                                                                       GATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC          >  SRR4302532.3539495/1‑94 (MQ=60)
                                                                                       GATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC          >  SRR4302532.1015445/1‑94 (MQ=60)
                                                                                         TTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        <  SRR4302532.1252170/94‑1 (MQ=60)
                                                                                           atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      >  SRR4302532.4531387/4‑94 (MQ=60)
                                                                                           atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      >  SRR4302532.3947302/4‑94 (MQ=60)
                                                                                            tcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302532.5101533/92‑1 (MQ=60)
                                                                                            T‑CCTGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302532.3240770/94‑1 (MQ=60)
                                                                                            C‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATG    >  SRR4302532.165283/1‑94 (MQ=60)
                                                                                              CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGC   <  SRR4302532.3134458/94‑1 (MQ=60)
                                                                                               C‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  <  SRR4302532.2283168/94‑1 (MQ=60)
                                                                                               C‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  <  SRR4302532.3694078/94‑1 (MQ=60)
                                                                                               C‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  SRR4302532.2631488/1‑94 (MQ=60)
                                                                                               |                                                                                              
TTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224832‑3225019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑