Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,224,926 +C coding (1052/1434 nt) ygjI → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,224,9241.C99.6% 826.5 / ‑4.5 226coding (1050/1434 nt)ygjIputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (110/115);  total (110/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224831‑3225011
                                                                                              |                                                                                       
cTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAt                                                                                          <  1:6593324/93‑1 (MQ=255)
 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                        >  2:3744428/1‑93 (MQ=255)
 tttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑c                                                                                        >  1:5797016/1‑93 (MQ=255)
  ttGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cc                                                                                       <  2:5212501/93‑1 (MQ=255)
     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                                    <  2:5534993/93‑4 (MQ=255)
     gTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccga                                                                                    <  2:3387225/93‑4 (MQ=255)
      tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑cccgat                                                                                   >  1:5257282/1‑89 (MQ=255)
      tAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑ccccac                                                                                   >  2:201130/1‑89 (MQ=255)
        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                                                                  >  1:417565/1‑93 (MQ=255)
        aGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGAAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                                                                  >  2:4667384/1‑93 (MQ=255)
        aGAAAACCGTCGAACTGAACGAAAACGGCGCTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGc                                                                                  >  2:142579/1‑93 (MQ=255)
          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                                                <  2:1654437/93‑1 (MQ=255)
          aaaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTc                                                                                <  2:1790181/93‑1 (MQ=255)
           aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                                                               <  1:2537212/93‑1 (MQ=255)
           aaaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCg                                                                               >  1:1728614/1‑93 (MQ=255)
            aaCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCgg                                                                              >  2:6191765/1‑93 (MQ=255)
             aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGt                                                                             <  2:2628407/93‑1 (MQ=255)
             aCCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGt                                                                             >  2:1754447/1‑93 (MQ=255)
                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                                      <  2:4838841/93‑1 (MQ=255)
                    aaCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATa                                                                      <  2:4248916/93‑1 (MQ=255)
                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                                     >  1:2054028/1‑93 (MQ=255)
                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                                     <  1:32953/93‑1 (MQ=255)
                     aCTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAc                                                                     >  1:5691425/1‑93 (MQ=255)
                      cTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATAcc                                                                    >  1:711840/1‑93 (MQ=255)
                        gAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCgg                                                                  >  1:1661179/1‑92 (MQ=255)
                          aCGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGc                                                                <  1:2401307/93‑1 (MQ=255)
                           cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                                               <  1:6365695/93‑1 (MQ=255)
                           cGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCa                                                               <  1:3032106/93‑1 (MQ=255)
                            gAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAg                                                              <  1:4913984/93‑1 (MQ=255)
                             aaaaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAgg                                                             >  1:5409115/1‑93 (MQ=255)
                               aaCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                           >  2:2298309/1‑93 (MQ=255)
                               aaCGGCGTTCCGGCGC‑GCCCAGCTTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGAt                                                           <  2:744696/93‑1 (MQ=255)
                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc                                                          <  1:4513563/93‑1 (MQ=255)
                                aCGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATc                                                          <  2:2123368/93‑1 (MQ=255)
                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                       >  2:6630657/1‑93 (MQ=255)
                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                       <  1:1506913/93‑1 (MQ=255)
                                   gCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtga                                                       >  1:2937120/1‑93 (MQ=255)
                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCGTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                     <  1:398143/93‑1 (MQ=255)
                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                     >  2:1148047/1‑93 (MQ=255)
                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                     >  1:798665/1‑93 (MQ=255)
                                     gTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCtgatg                                                     >  2:3580137/1‑93 (MQ=255)
                                      ttCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCCATACCGTGCAGAATCtgatga                                                    >  1:3528073/1‑93 (MQ=255)
                                       tCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGaa                                                   <  2:6411217/93‑1 (MQ=255)
                                         cggcgc‑gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATa                                                 <  1:1820376/93‑1 (MQ=255)
                                          ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                                                <  2:3125131/93‑1 (MQ=255)
                                          ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                                                <  2:3176428/93‑1 (MQ=255)
                                          ggcgc‑gcgcaGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAc                                                <  1:3867211/93‑1 (MQ=255)
                                             cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                                              <  2:2349403/90‑1 (MQ=255)
                                             cgcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACta                                              <  2:52382/90‑1 (MQ=255)
                                              gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                                             <  1:245754/91‑1 (MQ=255)
                                              gcgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtat                                             <  1:848941/91‑1 (MQ=255)
                                               cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                            >  1:5108623/2‑93 (MQ=255)
                                               cgcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatt                                            <  2:3518300/92‑1 (MQ=255)
                                                gcgcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACtatta                                           <  2:2156037/93‑1 (MQ=255)
                                                  gcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                                         <  2:3681321/93‑1 (MQ=255)
                                                  gcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                                         <  1:3017087/93‑1 (MQ=255)
                                                  gcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTattatt                                         >  2:3077448/1‑93 (MQ=255)
                                                  gcAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATATGATGAATACtattata                                         >  1:5717900/1‑92 (MQ=255)
                                                   cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                        >  1:566031/1‑93 (MQ=255)
                                                   cAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAttatta                                        <  1:2974374/93‑1 (MQ=255)
                                                    aGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTaa                                       <  1:3811540/93‑1 (MQ=255)
                                                     gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      >  1:4966674/1‑93 (MQ=255)
                                                     gCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAat                                      >  1:1586706/1‑93 (MQ=255)
                                                       gTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAatat                                    <  1:279343/93‑1 (MQ=255)
                                                         ggATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGa                                  >  1:6072893/1‑93 (MQ=255)
                                                          gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                 <  2:454896/93‑1 (MQ=255)
                                                          gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                 <  2:6068461/93‑1 (MQ=255)
                                                          gATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAc                                 >  2:697033/1‑93 (MQ=255)
                                                           aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                <  2:2983447/93‑1 (MQ=255)
                                                           aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                >  2:3978125/1‑93 (MQ=255)
                                                           aTCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAcc                                >  2:630371/1‑93 (MQ=255)
                                                            tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                               >  2:5512886/1‑93 (MQ=255)
                                                            tCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccg                               >  2:666578/1‑93 (MQ=255)
                                                             ccAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgc                              <  2:3402481/93‑1 (MQ=255)
                                                              cAGTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAccgcc                             >  2:5095907/1‑93 (MQ=255)
                                                                gTTCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACcgccgc                           >  2:6332775/1‑93 (MQ=255)
                                                                 ttCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          <  2:5644285/93‑1 (MQ=255)
                                                                 ttCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCa                          >  1:5770103/1‑93 (MQ=255)
                                                                  tCCTGATCTTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                         <  1:4266539/93‑1 (MQ=255)
                                                                  tCCTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAg                         <  1:6524681/93‑1 (MQ=255)
                                                                   ccTGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGc                        <  2:1985183/93‑1 (MQ=255)
                                                                     tGATCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGt                      >  2:2756513/1‑93 (MQ=255)
                                                                       aTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTcc                    >  1:1237055/1‑93 (MQ=255)
                                                                       aTCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCCTcc                    <  2:5928007/93‑1 (MQ=255)
                                                                        tCGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCa                   >  2:3913118/1‑93 (MQ=255)
                                                                         cGTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAt                  >  1:6051313/1‑93 (MQ=255)
                                                                          gTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATg                 <  1:103841/93‑1 (MQ=255)
                                                                          gTCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATg                 <  2:5025681/93‑1 (MQ=255)
                                                                           tCATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGc                <  1:3750206/93‑1 (MQ=255)
                                                                            cATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt               <  2:4404064/93‑1 (MQ=255)
                                                                            cATCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCt               >  1:4606278/1‑93 (MQ=255)
                                                                              tCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             <  2:5373666/93‑1 (MQ=255)
                                                                              tCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             <  1:561668/93‑1 (MQ=255)
                                                                              tCCCGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTc             <  2:4498677/93‑1 (MQ=255)
                                                                                ccGCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccg           >  1:741548/1‑93 (MQ=255)
                                                                                  gCTGATGATTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc         <  1:437707/93‑1 (MQ=255)
                                                                                  gCTGATGACTATCCCGATTCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTccgcc         <  1:940748/93‑1 (MQ=255)
                                                                                     gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      >  1:5349296/1‑93 (MQ=255)
                                                                                     gatgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGtt      >  1:4733985/1‑93 (MQ=255)
                                                                                       tgatTATCCCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAt    <  1:2302369/93‑1 (MQ=255)
                                                                                              |                                                                                       
CTTTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCGATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTC  >  NC_000913/3224831‑3225011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224833‑3225019
                                                                                              |                                                                                              
TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑C                                                                                              <  SRR4302547.5110882/94‑1 (MQ=60)
 TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                                                                              <  SRR4302547.2931288/94‑3 (MQ=60)
 TGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcg                                                                                              <  SRR4302547.4283799/94‑3 (MQ=60)
  GGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CCcga                                                                                             >  SRR4302547.5696485/1‑91 (MQ=60)
   GTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GAT                                                                                            >  SRR4302547.5365535/1‑94 (MQ=60)
    TAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATG                                                                                           >  SRR4302547.1299387/1‑94 (MQ=60)
     AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGC                                                                                          >  SRR4302547.3034910/1‑94 (MQ=60)
     AAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGC                                                                                          >  SRR4302547.425306/1‑94 (MQ=60)
        AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCG                                                                                       <  SRR4302547.2444395/94‑1 (MQ=60)
        AAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCG                                                                                       >  SRR4302547.1762575/1‑94 (MQ=60)
         AAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGG                                                                                      <  SRR4302547.2588476/94‑1 (MQ=60)
               TCTAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAA                                                                                <  SRR4302547.3297933/94‑1 (MQ=60)
                CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               <  SRR4302547.6183649/94‑1 (MQ=60)
                CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               <  SRR4302547.2579202/94‑1 (MQ=60)
                CGAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAAT                                                                               >  SRR4302547.683129/1‑94 (MQ=60)
                 GAACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATA                                                                              <  SRR4302547.3070842/94‑1 (MQ=60)
                  AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATAC                                                                             <  SRR4302547.4641860/94‑1 (MQ=60)
                  AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATAC                                                                             >  SRR4302547.2094992/1‑94 (MQ=60)
                  AACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATAC                                                                             >  SRR4302547.5808965/1‑94 (MQ=60)
                   ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            <  SRR4302547.33550/94‑1 (MQ=60)
                   ACTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACC                                                                            >  SRR4302547.725005/1‑94 (MQ=60)
                    CTGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCG                                                                           >  SRR4302547.3853854/1‑94 (MQ=60)
                     TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCCATACCGG                                                                          >  SRR4302547.1693932/1‑94 (MQ=60)
                     TGAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGT                                                                          >  SRR4302547.6163331/1‑94 (MQ=60)
                      GAACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTG                                                                         >  SRR4302547.2623570/1‑94 (MQ=60)
                        ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCA                                                                       <  SRR4302547.3985319/94‑1 (MQ=60)
                        ACGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCA                                                                       <  SRR4302547.2449939/94‑1 (MQ=60)
                         CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302547.3092403/94‑1 (MQ=60)
                         CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      >  SRR4302547.4403953/1‑94 (MQ=60)
                         CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      <  SRR4302547.6498497/94‑1 (MQ=60)
                         CGAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGCTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAG                                                                      >  SRR4302547.4804578/1‑94 (MQ=60)
                          GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCGCATACCGTGCAGG                                                                     >  SRR4302547.6080018/1‑94 (MQ=60)
                          GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGG                                                                     >  SRR4302547.5520954/1‑94 (MQ=60)
                          GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGG                                                                     >  SRR4302547.5396586/1‑94 (MQ=60)
                          GAAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGG                                                                     <  SRR4302547.5015745/94‑1 (MQ=60)
                           AAAACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGA                                                                    >  SRR4302547.1539005/1‑94 (MQ=60)
                              ACGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCT                                                                 <  SRR4302547.4606819/94‑1 (MQ=60)
                               CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTG                                                                >  SRR4302547.5832403/1‑94 (MQ=60)
                               CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTG                                                                <  SRR4302547.5743717/94‑1 (MQ=60)
                               CGGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTG                                                                <  SRR4302547.5863053/94‑1 (MQ=60)
                                GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGA                                                               >  SRR4302547.2995649/1‑94 (MQ=60)
                                GGCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGCTCCAATACCGTGCAGGATCTGA                                                               >  SRR4302547.2995650/1‑94 (MQ=60)
                                 GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGAT                                                              <  SRR4302547.6451977/94‑1 (MQ=60)
                                 GCGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGAT                                                              <  SRR4302547.1536949/94‑1 (MQ=60)
                                  CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302547.813784/1‑94 (MQ=60)
                                  CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302547.6444588/1‑94 (MQ=60)
                                  CGTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATG                                                             >  SRR4302547.438997/1‑94 (MQ=60)
                                   GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCGTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            <  SRR4302547.405549/94‑1 (MQ=60)
                                   GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCCATACCGTGCAGAATCTGATGA                                                            >  SRR4302547.3600112/1‑94 (MQ=60)
                                   GTTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGA                                                            <  SRR4302547.4157308/94‑1 (MQ=60)
                                    TTCCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAA                                                           >  SRR4302547.4560746/1‑94 (MQ=60)
                                      CCGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATA                                                         <  SRR4302547.486753/94‑1 (MQ=60)
                                       CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        <  SRR4302547.1856362/94‑1 (MQ=60)
                                       CGGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATAC                                                        <  SRR4302547.3944518/94‑1 (MQ=60)
                                        GGCGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACT                                                       <  SRR4302547.3945945/94‑1 (MQ=60)
                                          CGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAT                                                     >  SRR4302547.1787858/1‑94 (MQ=60)
                                          CGC‑GCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTAT                                                     >  SRR4302547.2879191/1‑94 (MQ=60)
                                            gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302547.865153/92‑1 (MQ=60)
                                            gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    <  SRR4302547.250345/92‑1 (MQ=60)
                                            gcGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATT                                                    >  SRR4302547.5213907/3‑94 (MQ=60)
                                             CGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTA                                                   <  SRR4302547.5158984/94‑1 (MQ=60)
                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATT                                                 <  SRR4302547.5576085/94‑1 (MQ=60)
                                               CGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATATGATGAATACTATTATA                                                 >  SRR4302547.5836002/1‑94 (MQ=60)
                                                GCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTA                                                >  SRR4302547.576212/1‑94 (MQ=60)
                                                GCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTA                                                <  SRR4302547.3077081/94‑1 (MQ=60)
                                                GCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTA                                                <  SRR4302547.3088852/94‑1 (MQ=60)
                                                 CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               <  SRR4302547.3033565/94‑1 (MQ=60)
                                                 CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               >  SRR4302547.4126600/1‑94 (MQ=60)
                                                 CAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAA                                               >  SRR4302547.5399900/1‑94 (MQ=60)
                                                  AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302547.1618247/1‑94 (MQ=60)
                                                  AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              >  SRR4302547.5069452/1‑94 (MQ=60)
                                                  AGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAAT                                              <  SRR4302547.3889216/94‑1 (MQ=60)
                                                   GCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATA                                             >  SRR4302547.1642327/1‑94 (MQ=60)
                                                     GTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATG                                           <  SRR4302547.284552/94‑1 (MQ=60)
                                                      TGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGA                                          >  SRR4302547.6198871/1‑94 (MQ=60)
                                                       GGATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTGCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGAC                                         >  SRR4302547.6693869/1‑94 (MQ=60)
                                                        GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        >  SRR4302547.5859357/1‑94 (MQ=60)
                                                        GATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACC                                        <  SRR4302547.897819/94‑1 (MQ=60)
                                                         ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       <  SRR4302547.1885771/94‑1 (MQ=60)
                                                         ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       <  SRR4302547.3658251/94‑1 (MQ=60)
                                                         ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCG                                       >  SRR4302547.5031986/1‑94 (MQ=60)
                                                         ATCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAAGACTATTATTAATATGACCG                                       >  SRR4302547.814765/1‑94 (MQ=60)
                                                          TCCAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGC                                      <  SRR4302547.1716201/94‑1 (MQ=60)
                                                            CAGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCG                                    <  SRR4302547.3771623/94‑1 (MQ=60)
                                                             AGTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGC                                   <  SRR4302547.72586/94‑1 (MQ=60)
                                                              GTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCA                                  <  SRR4302547.4832445/94‑1 (MQ=60)
                                                              GTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCA                                  >  SRR4302547.5889443/1‑94 (MQ=60)
                                                              GTTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCA                                  <  SRR4302547.2571611/94‑1 (MQ=60)
                                                               TTCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAG                                 <  SRR4302547.2458872/94‑1 (MQ=60)
                                                                TCCTGATCTTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                <  SRR4302547.4353988/94‑1 (MQ=60)
                                                                TCCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGC                                <  SRR4302547.6660512/94‑1 (MQ=60)
                                                                 CCTGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCG                               >  SRR4302547.5517374/1‑94 (MQ=60)
                                                                   TGATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTC                             >  SRR4302547.2047257/1‑94 (MQ=60)
                                                                    GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302547.1262029/1‑94 (MQ=60)
                                                                    GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302547.1543536/1‑94 (MQ=60)
                                                                    GATCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCC                            >  SRR4302547.806535/1‑94 (MQ=60)
                                                                      TCGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCAT                          >  SRR4302547.6176847/1‑94 (MQ=60)
                                                                       CGTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATG                         <  SRR4302547.6566581/94‑1 (MQ=60)
                                                                        GTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGC                        <  SRR4302547.105737/94‑1 (MQ=60)
                                                                        GTCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGC                        >  SRR4302547.5901830/1‑94 (MQ=60)
                                                                         TCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       <  SRR4302547.578942/94‑1 (MQ=60)
                                                                         TCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       <  SRR4302547.3826747/94‑1 (MQ=60)
                                                                         TCATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCT                       >  SRR4302547.4701604/1‑94 (MQ=60)
                                                                           ATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTC                     >  SRR4302547.2253481/1‑94 (MQ=60)
                                                                           ATCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTC                     >  SRR4302547.3319492/1‑94 (MQ=60)
                                                                            tccggctgatgaggatctC‑GATGCTCGGTTCCAATCCCGTGCAGGATCTGATGAAGACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302547.3780880/76‑1 (MQ=60)
                                                                            TCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302547.571786/94‑1 (MQ=60)
                                                                            TCCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCC                    <  SRR4302547.3424962/94‑1 (MQ=60)
                                                                             CCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCG                   >  SRR4302547.5799402/1‑94 (MQ=60)
                                                                             CCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCG                   >  SRR4302547.755474/1‑94 (MQ=60)
                                                                             CCCGCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCG                   <  SRR4302547.1389058/94‑1 (MQ=60)
                                                                                GCTGATGTTTAG‑CCTGATGCTCGATTCCAATGCCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCTCAGCGTCCATGCTTCCGCCG                <  SRR4302547.35319/94‑1 (MQ=60)
                                                                                GCTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCG                <  SRR4302547.445869/94‑1 (MQ=60)
                                                                                GCTGATGACTATCCC‑GATTCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCG                <  SRR4302547.958972/94‑1 (MQ=60)
                                                                                 CTGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGT               >  SRR4302547.2373074/1‑94 (MQ=60)
                                                                                  TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              >  SRR4302547.5459596/1‑94 (MQ=60)
                                                                                  TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              >  SRR4302547.4832102/1‑94 (MQ=60)
                                                                                  TGATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTT              >  SRR4302547.6685707/1‑94 (MQ=60)
                                                                                    ATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAT            <  SRR4302547.1097602/94‑1 (MQ=60)
                                                                                    ATGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTAT            <  SRR4302547.4697033/94‑1 (MQ=60)
                                                                                    ATGATTATCCC‑GATGCTCGGTTCCAATACCGCGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTACGCCGTTAT            >  SRR4302547.725732/1‑94 (MQ=60)
                                                                                     TGATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATT           <  SRR4302547.2349027/94‑1 (MQ=60)
                                                                                       ATTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCA         <  SRR4302547.849685/94‑1 (MQ=60)
                                                                                        TTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCATCGTCCATGCTTCCGCCGTTATTCAT        <  SRR4302547.6540539/94‑1 (MQ=60)
                                                                                        TTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        >  SRR4302547.1658665/1‑94 (MQ=60)
                                                                                        TTATCCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCAT        <  SRR4302547.733439/94‑1 (MQ=60)
                                                                                          atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      <  SRR4302547.1978187/91‑1 (MQ=60)
                                                                                          atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      <  SRR4302547.1110855/91‑1 (MQ=60)
                                                                                          atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      >  SRR4302547.1544859/4‑94 (MQ=60)
                                                                                          atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      <  SRR4302547.1586371/91‑1 (MQ=60)
                                                                                          atcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCA      >  SRR4302547.2863252/4‑94 (MQ=60)
                                                                                           tcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     >  SRR4302547.6184715/3‑94 (MQ=60)
                                                                                           tcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     <  SRR4302547.1299284/92‑1 (MQ=60)
                                                                                           tcCC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCAT     >  SRR4302547.663085/3‑94 (MQ=60)
                                                                                           C‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATG    >  SRR4302547.2276328/1‑94 (MQ=60)
                                                                                           C‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATG    <  SRR4302547.1246786/94‑1 (MQ=60)
                                                                                             CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGC   >  SRR4302547.5590203/1‑94 (MQ=60)
                                                                                              C‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  SRR4302547.1706582/1‑94 (MQ=60)
                                                                                              |                                                                                              
TTGGTAAGAAAACCGTCGAACTGAACGAAAACGGCGTTCCGGCGCGGCGCAGCGTGGATCCAGTTCCTGATCGTCATCCCGCTGATGATTAT‑CC‑GATGCTCGGTTCCAATACCGTGCAGGATCTGATGAATACTATTATTAATATGACCGCCGCAGCGTCCATGCTTCCGCCGTTATTCATCATGCT  >  NC_000913/3224833‑3225019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑