Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,893	+C	coding (405/423 nt)	yhaQ ←	putative L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,891	1	.	C	99.5%	776.5 / ‑4.0	217	coding (407/423 nt)	yhaQ	putative L‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (119/97); total (120/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 236 total aligned reads displayed.

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256800‑3256978

tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                         >  1:2281905/1‑93 (MQ=255)

   cGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccct                                                                                      <  2:1327510/93‑3 (MQ=255)

     aCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cccttc                                                                                    >  2:5765110/1‑89 (MQ=255)

     aCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cccttc                                                                                    <  2:1218533/93‑5 (MQ=255)

     aCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cccttc                                                                                    <  1:2353728/93‑5 (MQ=255)

       aTCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCa                                                                                  >  2:2112940/1‑93 (MQ=255)

        tCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAg                                                                                 >  1:630647/1‑93 (MQ=255)

        tCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAg                                                                                 >  1:3714296/1‑93 (MQ=255)

         cGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAgg                                                                                <  1:4811925/93‑1 (MQ=255)

          gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGc                                                                               <  1:5594206/93‑1 (MQ=255)

          gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGc                                                                               <  1:743445/93‑1 (MQ=255)

               aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGa                                                                          <  1:2881885/93‑1 (MQ=255)

                  ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATcc                                                                       >  1:1211972/1‑93 (MQ=255)

                   gCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTACAGGCAGTGATCCg                                                                      >  1:3218895/1‑93 (MQ=255)

                     cGAAGTGTTCTTCTTCGACCATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCa                                                                    <  1:5467651/93‑1 (MQ=255)

                     cGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCa                                                                    >  2:1770025/1‑93 (MQ=255)

                     cGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCa                                                                    <  2:2363986/93‑1 (MQ=255)

                      gAAGTGTTCTTCTTCGACAATACACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGATCTTCCTGTGCC‑TTCCAGGCAGTGATCCGCAt                                                                   >  1:6121018/1‑93 (MQ=255)

                      gAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCAt                                                                   >  2:4801134/1‑93 (MQ=255)

                       aaGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCAtt                                                                  <  2:3559987/93‑1 (MQ=255)

                         gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGATCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTcc                                                                >  2:786090/1‑93 (MQ=255)

                          tgtTCTTCTTCGAAAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCg                                                               <  1:1993978/93‑1 (MQ=255)

                           gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGt                                                              >  2:4920628/1‑93 (MQ=255)

                            ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCGTTCCAGGCAGTGATCCGCAGTCCGtt                                                             >  2:5679584/1‑93 (MQ=255)

                             tcttcttcGACAATAAACCCGCCGCCGACAGAGGAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGGtc                                                            >  1:936753/1‑93 (MQ=255)

                               ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtctc                                                          >  1:4040324/1‑93 (MQ=255)

                               ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtctc                                                          >  2:3224534/1‑93 (MQ=255)

                                tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         >  2:5723403/1‑93 (MQ=255)

                                tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         <  2:1926021/93‑1 (MQ=255)

                                 cttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCAt                                                        >  1:3928212/1‑93 (MQ=255)

                                 cttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCAt                                                        >  2:515437/1‑93 (MQ=255)

                                  ttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATg                                                       >  2:3533594/1‑93 (MQ=255)

                                    cGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGc                                                     <  2:2634168/93‑1 (MQ=255)

                                     gACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCg                                                    <  1:1523531/93‑1 (MQ=255)

                                     gACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCg                                                    >  2:385432/1‑93 (MQ=255)

                                      aCAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGa                                                   <  1:4208440/93‑1 (MQ=255)

                                       cAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg                                                  <  1:1123107/93‑1 (MQ=255)

                                       cAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg                                                  >  2:5330980/1‑93 (MQ=255)

                                        aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  1:4932216/1‑93 (MQ=255)

                                        aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  1:5788446/1‑93 (MQ=255)

                                        aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  2:2274445/1‑93 (MQ=255)

                                        aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 >  1:3571822/1‑93 (MQ=255)

                                        aaTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                 <  2:5440670/93‑1 (MQ=255)

                                          tAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa                                               >  2:5308525/1‑93 (MQ=255)

                                           aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa                                              >  1:1918627/1‑93 (MQ=255)

                                             aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa                                            >  2:4360039/1‑93 (MQ=255)

                                             aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa                                            <  2:5628496/93‑1 (MQ=255)

                                              cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAt                                           >  2:4292004/1‑93 (MQ=255)

                                              cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAt                                           <  2:155757/93‑1 (MQ=255)

                                                cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                         <  2:4085407/93‑1 (MQ=255)

                                                cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                         >  2:3990992/1‑93 (MQ=255)

                                                  ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                       >  1:3893338/1‑93 (MQ=255)

                                                   cgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg                                      <  2:3398979/93‑1 (MQ=255)

                                                    gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCggg                                     >  2:4695058/1‑93 (MQ=255)

                                                     ccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGa                                    <  2:917787/93‑1 (MQ=255)

                                                       gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                  >  1:3914227/1‑93 (MQ=255)

                                                        aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                 <  1:1879941/93‑1 (MQ=255)

                                                         cAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa                                >  2:1517100/1‑93 (MQ=255)

                                                          agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa                               <  1:527402/93‑1 (MQ=255)

                                                           gagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                              <  1:16756/93‑1 (MQ=255)

                                                           gagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                              >  1:3027799/1‑93 (MQ=255)

                                                           gagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGAAGAGACCCGCATTCCTTTCTCTTGGCGTGGCAACATTTCTGGATCGacg                              >  2:2706118/1‑91 (MQ=255)

                                                            agTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGa                             <  1:5229141/93‑1 (MQ=255)

                                                             gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGaa                            >  1:1314896/1‑92 (MQ=255)

                                                             gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt                            >  2:5515622/1‑93 (MQ=255)

                                                              tAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATa                           <  2:3162886/93‑1 (MQ=255)

                                                               aaTAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa                          <  1:1639785/93‑1 (MQ=255)

                                                               aaTAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa                          <  2:2814913/93‑1 (MQ=255)

                                                               aaTAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa                          <  1:328883/93‑1 (MQ=255)

                                                                aTAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt                         >  1:4726912/1‑93 (MQ=255)

                                                                 tAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        >  1:4391187/1‑93 (MQ=255)

                                                                   ggTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                      <  1:2755868/93‑1 (MQ=255)

                                                                    gTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCACCATTTCGGGATGGAAGATAATGTTc                     <  2:2562475/93‑1 (MQ=255)

                                                                     ttttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                    >  2:1681596/1‑93 (MQ=255)

                                                                      tttACTTAATAGCTCGTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAGGTTCtt                   <  1:5847754/93‑1 (MQ=255)

                                                                       ttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGATGCAACATTTCGGGATGGAAGATAATGTTCttt                  <  2:1042164/93‑1 (MQ=255)

                                                                       ttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                  <  2:706680/93‑1 (MQ=255)

                                                                        tACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTg                 >  2:4258319/1‑93 (MQ=255)

                                                                        tACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTg                 >  1:2122943/1‑93 (MQ=255)

                                                                         aCTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                >  2:4563643/1‑93 (MQ=255)

                                                                         aCTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                <  2:3506784/93‑1 (MQ=255)

                                                                          cTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCt               <  1:1271833/93‑1 (MQ=255)

                                                                          cTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCt               >  2:4998704/1‑93 (MQ=255)

                                                                            tAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             <  2:947766/93‑1 (MQ=255)

                                                                            tAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             >  2:4025328/1‑93 (MQ=255)

                                                                            tAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             <  2:731224/93‑1 (MQ=255)

                                                                            tAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             >  1:1083054/1‑93 (MQ=255)

                                                                            tAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             >  1:3142430/1‑93 (MQ=255)

                                                                                aGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTGGCTACAGGa         <  2:6058398/93‑1 (MQ=255)

                                                                                 gCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaa        >  1:305999/1‑93 (MQ=255)

                                                                                   tctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      >  2:4323121/1‑93 (MQ=255)

                                                                                    ctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt     >  2:2934786/1‑93 (MQ=255)

                                                                                    ctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt     <  2:5961003/93‑1 (MQ=255)

                                                                                    ctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt     <  2:2448280/93‑1 (MQ=255)

                                                                                     ttCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc    >  1:1751978/1‑93 (MQ=255)

                                                                                     ttCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACACGAAAATc    >  1:5044179/1‑93 (MQ=255)

                                                                                     ttCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGAAAATc    <  2:5966441/93‑1 (MQ=255)

                                                                                      tCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATca   >  1:5761374/1‑93 (MQ=255)

                                                                                       ccTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaa  >  1:2203407/1‑93 (MQ=255)

                                                                                       ccTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaa  <  1:903262/93‑1 (MQ=255)

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256800‑3256978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  NC_000913/3256802‑3256986

TCGACATCGTGCGACAGGCCGAAGTGTTCTCCTGCAAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCCT                                                                                             <  SRR4302532.575851/94‑1 (MQ=60)

   ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCC                                                                                           <  SRR4302532.2347659/94‑1 (MQ=60)

   ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCC                                                                                           <  SRR4302532.2432264/94‑1 (MQ=60)

   ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCC                                                                                           <  SRR4302532.2432265/94‑1 (MQ=60)

     cacggggcACAGGCCGAAGGTTTCTTCTTCGACAAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCA                                                                                          <  SRR4302532.5779529/86‑1 (MQ=60)

     ATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAG                                                                                         <  SRR4302532.6133229/94‑1 (MQ=60)

     ATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAG                                                                                         >  SRR4302532.652672/1‑94 (MQ=60)

     ATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAG                                                                                         >  SRR4302532.3838969/1‑94 (MQ=60)

     ATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCctttcag                                                                                        >  SRR4302532.4346670/1‑87 (MQ=60)

       CGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGC                                                                                       <  SRR4302532.4977635/94‑1 (MQ=60)

        GTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCA                                                                                      <  SRR4302532.5788397/94‑1 (MQ=60)

        GTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCA                                                                                      <  SRR4302532.768866/94‑1 (MQ=60)

         TGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAG                                                                                     <  SRR4302532.3277327/94‑1 (MQ=60)

             ACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGAT                                                                                 <  SRR4302532.2978206/94‑1 (MQ=60)

              CAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATC                                                                                >  SRR4302532.1635703/1‑94 (MQ=60)

               AGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCC                                                                               >  SRR4302532.1252521/1‑94 (MQ=60)

                GGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTACAGGCAGTGATCCG                                                                              >  SRR4302532.3325775/1‑94 (MQ=60)

                  CCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCA                                                                            >  SRR4302532.3919466/1‑94 (MQ=60)

                   CGAAGTGTTCTTCTTCGAC‑CATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAT                                                                           <  SRR4302532.5657220/94‑1 (MQ=60)

                   CGAAGTGTTCTTCTTCGAC‑AATACACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGATCTTCCTGTGCCTTCCAGGCAGTGATCCGCAT                                                                           >  SRR4302532.6332254/1‑94 (MQ=60)

                   CGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAT                                                                           >  SRR4302532.6332253/1‑94 (MQ=60)

                      AGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCC                                                                        >  SRR4302532.1021308/1‑94 (MQ=60)

                        TGTTCTTCTTCGAA‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGT                                                                      <  SRR4302532.2060357/94‑1 (MQ=60)

                         GTTCTTCTTCGAC‑AATAAACCCGCCGCCGAGAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTT                                                                     >  SRR4302532.4016816/1‑94 (MQ=60)

                         GTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTT                                                                     >  SRR4302532.5982998/1‑94 (MQ=60)

                          ttatgctgatAC‑AATAAACCCGCCCCCGACAGAGCAATAGGTTTTACTTAAGAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTC                                                                    <  SRR4302532.2353649/84‑1 (MQ=60)

                          TTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTC                                                                    >  SRR4302532.3678589/1‑94 (MQ=60)

                          TTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGGAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGGTC                                                                    >  SRR4302532.968850/1‑94 (MQ=60)

                            CTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTC                                                                  >  SRR4302532.4176419/1‑94 (MQ=60)

                            CTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTC                                                                  <  SRR4302532.4808407/94‑1 (MQ=60)

                              TCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                >  SRR4302532.5831411/1‑94 (MQ=60)

                              TCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                >  SRR4302532.4060568/1‑94 (MQ=60)

                              TCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                >  SRR4302532.4060569/1‑94 (MQ=60)

                              TCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                >  SRR4302532.1972294/1‑94 (MQ=60)

                               CTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                               >  SRR4302532.3505217/1‑94 (MQ=60)

                                 TGGAC‑AAAAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGC                                                             <  SRR4302532.5142962/94‑1 (MQ=60)

                                 TCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGC                                                             <  SRR4302532.5245988/94‑1 (MQ=60)

                                 TCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGC                                                             >  SRR4302532.1205515/1‑94 (MQ=60)

                                   GAC‑AATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGA                                                           <  SRR4302532.1574995/94‑1 (MQ=60)

                                    AC‑AATAAACCGGACGCGGACAGAGTAATAGGTTTTGCTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAG                                                          <  SRR4302532.4349700/94‑1 (MQ=60)

                                    AC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAG                                                          <  SRR4302532.1699604/94‑1 (MQ=60)

                                    AC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAG                                                          <  SRR4302532.4349701/94‑1 (MQ=60)

                                     C‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                         >  SRR4302532.5101579/1‑94 (MQ=60)

                                     C‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                         >  SRR4302532.3691355/1‑94 (MQ=60)

                                     C‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                         <  SRR4302532.1160606/94‑1 (MQ=60)

                                     C‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                         >  SRR4302532.4657908/1‑94 (MQ=60)

                                     C‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGG                                                         >  SRR4302532.5989710/1‑94 (MQ=60)

                                       AATAATCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                        <  SRR4302532.6336578/94‑1 (MQ=60)

                                       AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                        >  SRR4302532.1616470/1‑94 (MQ=60)

                                       AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                        >  SRR4302532.1520403/1‑94 (MQ=60)

                                        ATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                       <  SRR4302532.952024/94‑1 (MQ=60)

                                         TAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAA                                                      >  SRR4302532.1982437/1‑94 (MQ=60)

                                           AACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACA                                                    <  SRR4302532.3168347/94‑1 (MQ=60)

                                            ACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAT                                                   >  SRR4302532.5441276/1‑94 (MQ=60)

                                               CGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTC                                                <  SRR4302532.3139742/94‑1 (MQ=60)

                                                GCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCG                                               <  SRR4302532.1809984/94‑1 (MQ=60)

                                                GCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCG                                               <  SRR4302532.2114034/94‑1 (MQ=60)

                                                GCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCG                                               >  SRR4302532.4024681/1‑94 (MQ=60)

                                                    CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           <  SRR4302532.2462494/94‑1 (MQ=60)

                                                     CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          >  SRR4302532.4046147/1‑94 (MQ=60)

                                                     CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          >  SRR4302532.1849971/1‑94 (MQ=60)

                                                     CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          >  SRR4302532.1849970/1‑94 (MQ=60)

                                                     CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          <  SRR4302532.6031025/94‑1 (MQ=60)

                                                     CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          <  SRR4302532.1266130/94‑1 (MQ=60)

                                                      GACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGG                                         >  SRR4302532.3693274/1‑94 (MQ=60)

                                                      GACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGG                                         >  SRR4302532.403102/1‑94 (MQ=60)

                                                       ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                        <  SRR4302532.1942252/94‑1 (MQ=60)

                                                        CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       >  SRR4302532.3049848/1‑94 (MQ=60)

                                                        CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       >  SRR4302532.5095438/1‑94 (MQ=60)

                                                         AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      >  SRR4302532.3127707/1‑94 (MQ=60)

                                                         AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      <  SRR4302532.545804/94‑1 (MQ=60)

                                                          GAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGA                                     <  SRR4302532.17443/94‑1 (MQ=60)

                                                           AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                    <  SRR4302532.5409479/94‑1 (MQ=60)

                                                           AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                    >  SRR4302532.4061690/1‑94 (MQ=60)

                                                           AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAA                                    >  SRR4302532.1358857/1‑94 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   >  SRR4302532.4123257/1‑94 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   >  SRR4302532.799300/1‑94 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   >  SRR4302532.2877061/1‑94 (MQ=60)

                                                             TAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                  <  SRR4302532.2232464/94‑1 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 <  SRR4302532.340293/94‑1 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 <  SRR4302532.1695300/94‑1 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302532.4888234/1‑94 (MQ=60)

                                                               ATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATG                                >  SRR4302532.127345/1‑94 (MQ=60)

                                                               ATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATG                                >  SRR4302532.4540501/1‑94 (MQ=60)

                                                                TAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               <  SRR4302532.131798/94‑1 (MQ=60)

                                                                  GGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTC                             <  SRR4302532.2848423/94‑1 (MQ=60)

                                                                  GGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTC                             >  SRR4302532.5955014/1‑94 (MQ=60)

                                                                    TTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT                           >  SRR4302532.3363668/1‑94 (MQ=60)

                                                                    TTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT                           <  SRR4302532.2281934/94‑1 (MQ=60)

                                                                     TTTACTTAATAGCTCGTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAGGTTCTTT                          <  SRR4302532.6050666/94‑1 (MQ=60)

                                                                      TTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTG                         >  SRR4302532.2193861/1‑94 (MQ=60)

                                                                      TTACTTAATAGCTCTTCCTGTCCCTTCCAAGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTG                         >  SRR4302532.3216559/1‑94 (MQ=60)

                                                                       TACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGC                        >  SRR4302532.4399038/1‑94 (MQ=60)

                                                                         CTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTA                      <  SRR4302532.1314371/94‑1 (MQ=60)

                                                                          TTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                     >  SRR4302532.2899942/1‑94 (MQ=60)

                                                                          TTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                     >  SRR4302532.1119346/1‑94 (MQ=60)

                                                                          TTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                     >  SRR4302532.3246718/1‑94 (MQ=60)

                                                                           TAATAGCTCGTCCTGTCCCTTCCAGGCAGTGATCCGCTTTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACA                    <  SRR4302532.2044638/94‑1 (MQ=60)

                                                                            AATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                   <  SRR4302532.5140094/94‑1 (MQ=60)

                                                                            AATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                   <  SRR4302532.4921255/94‑1 (MQ=60)

                                                                             ATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGG                  >  SRR4302532.878356/1‑94 (MQ=60)

                                                                             ATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGG                  >  SRR4302532.1152072/1‑94 (MQ=60)

                                                                               AGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAA                >  SRR4302532.316615/1‑94 (MQ=60)

                                                                                 CTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAA              >  SRR4302532.5474424/1‑94 (MQ=60)

                                                                                  TCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAT             >  SRR4302532.2141234/1‑94 (MQ=60)

                                                                                   CTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATC            >  SRR4302532.1810831/1‑94 (MQ=60)

                                                                                   CTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACACGAAAATC            >  SRR4302532.5217217/1‑94 (MQ=60)

                                                                                    ttactgtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCA           <  SRR4302532.3200017/86‑1 (MQ=60)

                                                                                    TTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCA           >  SRR4302532.5961884/1‑94 (MQ=60)

                                                                                     TCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA          <  SRR4302532.5533247/94‑1 (MQ=60)

                                                                                     TCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA          >  SRR4302532.2276716/1‑94 (MQ=60)

                                                                                      CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         <  SRR4302532.934067/94‑1 (MQ=60)

                                                                                      CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302532.1049598/1‑94 (MQ=60)

                                                                                      CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302532.146983/1‑94 (MQ=60)

                                                                                      CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302532.4290008/1‑94 (MQ=60)

                                                                                      CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302532.3366638/1‑94 (MQ=60)

                                                                                      CCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302532.3922188/1‑94 (MQ=60)

                                                                                       CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        >  SRR4302532.1196134/1‑94 (MQ=60)

                                                                                       CTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302532.4970953/94‑1 (MQ=60)

                                                                                        TGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       >  SRR4302532.1499775/1‑94 (MQ=60)

                                                                                        TGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       >  SRR4302532.4536215/1‑94 (MQ=60)

                                                                                         gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      >  SRR4302532.4062447/4‑94 (MQ=60)

                                                                                         gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      >  SRR4302532.3334081/4‑94 (MQ=60)

                                                                                         gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      >  SRR4302532.3334082/4‑94 (MQ=60)

                                                                                         gtcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      >  SRR4302532.6330856/4‑94 (MQ=60)

                                                                                          tcCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGCGGCAACATTTCGGGATGGAAGATAATGGTCCTTGCTACAGGAAAATCAACAATA     >  SRR4302532.4654187/3‑94 (MQ=60)

                                                                                            CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   <  SRR4302532.552624/94‑1 (MQ=60)

                                                                                            CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   >  SRR4302532.5233802/1‑94 (MQ=60)

                                                                                             CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  <  SRR4302532.3731493/94‑1 (MQ=60)

                                                                                             CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  <  SRR4302532.631426/94‑1 (MQ=60)

                                                                                             CTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  SRR4302532.2534259/1‑94 (MQ=60)

                                                                                             CTTCCAGACAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  SRR4302532.4627496/1‑94 (MQ=60)

TCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGAC‑AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  NC_000913/3256802‑3256986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`