Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,197	A→C	R145S (AGA→AGC)	agaA →	putative N‑acetylgalactosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,197	0	A	C	100.0%	815.9 / NA	229	R145S (AGA→AGC)	agaA	putative N‑acetylgalactosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (94/135); total (94/135)

Only 100 of 237 total aligned reads displayed.

GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         <  2:15970/93‑1 (MQ=255)

ggAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGt                                                                                         >  1:3868332/1‑93 (MQ=255)

 gAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc                                                                                        <  1:6032809/93‑1 (MQ=255)

 gAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTc                                                                                        >  1:3959544/1‑93 (MQ=255)

   aGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGcgtcgt                                                                                      >  2:3471732/1‑93 (MQ=255)

    gCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt                                                                                     >  1:5437114/1‑93 (MQ=255)

    gCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt                                                                                     >  1:3262226/1‑93 (MQ=255)

    accatccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGtt                                                                                     <  1:5051849/92‑1 (MQ=255)

      catccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc                                                                                   <  2:5867900/93‑1 (MQ=255)

      catccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgc                                                                                   >  2:331876/1‑93 (MQ=255)

        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 >  2:1863456/1‑93 (MQ=255)

        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 >  2:2193574/1‑93 (MQ=255)

        tccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 <  1:3396054/93‑1 (MQ=255)

        tccatATGGCGTCGCTGCATCCGACGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTgcgc                                                                                 <  1:4299199/93‑1 (MQ=255)

         ccatATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCt                                                                                >  1:3440319/1‑93 (MQ=255)

          catATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTg                                                                               <  1:5567118/93‑1 (MQ=255)

           atatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTgg                                                                              >  2:3919619/1‑93 (MQ=255)

            tatGGCGTCGGTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGTATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGa                                                                             <  1:1426628/93‑1 (MQ=255)

            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa                                                                             >  1:4786406/1‑93 (MQ=255)

            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa                                                                             >  2:1898797/1‑93 (MQ=255)

            tatGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGa                                                                             <  2:6108700/93‑1 (MQ=255)

             atGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAt                                                                            <  2:2032183/93‑1 (MQ=255)

               ggCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAg                                                                          <  1:2895609/93‑1 (MQ=255)

               ggCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGCATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGAtgc                                                                          >  2:5961231/1‑91 (MQ=255)

                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         >  2:4590704/1‑93 (MQ=255)

                gCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGc                                                                         >  1:1133637/1‑93 (MQ=255)

                 cggcGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCg                                                                        <  1:4781012/90‑1 (MQ=255)

                 cGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCg                                                                        <  2:4739473/93‑1 (MQ=255)

                 cGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCg                                                                        <  2:4308627/93‑1 (MQ=255)

                  gTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCgg                                                                       <  1:2987970/93‑1 (MQ=255)

                   tCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCggg                                                                      >  1:4686944/1‑93 (MQ=255)

                    cGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc                                                                     >  1:1446100/1‑93 (MQ=255)

                    cGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc                                                                     <  2:4441059/93‑1 (MQ=255)

                    cGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGc                                                                     <  1:3119894/93‑1 (MQ=255)

                     gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCt                                                                    >  2:5178720/1‑93 (MQ=255)

                     gCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCt                                                                    >  2:5693733/1‑93 (MQ=255)

                      cTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa                                                                   >  1:4853017/1‑93 (MQ=255)

                      cTGCATCCGGAGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTa                                                                   >  1:1757350/1‑93 (MQ=255)

                       tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc                                                                  >  2:2148842/1‑93 (MQ=255)

                       tGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAc                                                                  <  1:612013/93‑1 (MQ=255)

                        gCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACa                                                                 <  1:912106/93‑1 (MQ=255)

                         cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt                                                                <  2:5647842/93‑1 (MQ=255)

                         cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt                                                                <  1:3604285/93‑1 (MQ=255)

                         cATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAt                                                                >  2:4272257/1‑93 (MQ=255)

                               gcgcGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCTGGCTACATGTGcaa                                                          <  1:4339917/93‑1 (MQ=255)

                                cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac                                                         <  2:3948173/93‑1 (MQ=255)

                                cgcgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaac                                                         <  1:5388556/93‑1 (MQ=255)

                                  cgAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGcaacaa                                                       >  1:4532104/1‑93 (MQ=255)

                                     aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc                                                    >  2:5204086/1‑93 (MQ=255)

                                     aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc                                                    >  1:1189551/1‑93 (MQ=255)

                                     aTGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATc                                                    >  1:2461798/1‑93 (MQ=255)

                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   >  1:5775460/1‑93 (MQ=255)

                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   <  1:4076062/93‑1 (MQ=255)

                                      tGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCt                                                   <  1:502181/93‑1 (MQ=255)

                                        cTGGGTGTTGATGGTGTTCTGGGATCGCTTAATCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgt                                                 <  2:5900237/93‑2 (MQ=255)

                                        cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg                                                 <  1:5834523/93‑1 (MQ=255)

                                        cTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTgg                                                 >  1:1879300/1‑93 (MQ=255)

                                         tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa                                                >  2:801386/1‑93 (MQ=255)

                                         tGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGa                                                <  2:238794/93‑1 (MQ=255)

                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  1:3499132/93‑1 (MQ=255)

                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  2:2293551/93‑1 (MQ=255)

                                              gtTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAg                                           <  1:639114/93‑1 (MQ=255)

                                               ttGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAgg                                          <  1:6090641/93‑1 (MQ=255)

                                                 gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt                                        >  1:3297679/1‑93 (MQ=255)

                                                 gATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGt                                        <  1:103887/93‑1 (MQ=255)

                                                  aTGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTc                                       <  2:6127239/93‑1 (MQ=255)

                                                    ggTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCaa                                     >  2:759855/1‑93 (MQ=255)

                                                     gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAt                                    <  1:2781244/93‑1 (MQ=255)

                                                     gtgtTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAt                                    >  2:5858358/1‑93 (MQ=255)

                                                      ggtTCTGGCAGCGCTTAAACCGGGCAACCGCGCGCGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCACGGTCAAtt                                   <  2:1500734/92‑1 (MQ=255)

                                                       tttCTGGGATCGCTTAAACCGGGCAGACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTa                                  <  1:440514/92‑1 (MQ=255)

                                                        ttCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAg                                 >  2:1057625/1‑93 (MQ=255)

                                                         tCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGCGCAACAAATCTGGATTCAGGGTCAATTAGc                                >  1:1944301/1‑93 (MQ=255)

                                                          cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt                               <  1:5936898/93‑1 (MQ=255)

                                                          cTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCt                               <  1:313237/93‑1 (MQ=255)

                                                           tGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCtt                              <  1:2932286/93‑1 (MQ=255)

                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  2:3255935/93‑1 (MQ=255)

                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  1:3808838/93‑1 (MQ=255)

                                                            gggATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTc                             <  1:2445692/93‑1 (MQ=255)

                                                               aTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGtt                          <  1:3945325/93‑1 (MQ=255)

                                                                  gCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTg                       >  2:2398546/1‑93 (MQ=255)

                                                                   cTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGa                      >  2:2486627/1‑93 (MQ=255)

                                                                     tAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATa                    <  1:2258628/93‑1 (MQ=255)

                                                                      aaaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAg                   >  1:1497532/1‑93 (MQ=255)

                                                                       aaCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGt                  <  1:2029631/93‑1 (MQ=255)

                                                                           gggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGc              >  2:3167448/1‑93 (MQ=255)

                                                                            ggCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCt             >  1:2358983/1‑93 (MQ=255)

                                                                             gCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTc            >  2:1241407/1‑93 (MQ=255)

                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          >  2:313091/1‑93 (MQ=255)

                                                                               aaaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCt          <  2:2590123/93‑1 (MQ=255)

                                                                                accgcgCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  1:2631981/91‑1 (MQ=255)

                                                                                aaCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCtt         <  2:3716056/93‑1 (MQ=255)

                                                                                  cgcgCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTa       <  2:1012981/93‑1 (MQ=255)

                                                                                   gcgcCAGCGTCGTTGCGCTGGATAGCGGGGTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      <  2:2784525/93‑1 (MQ=255)

                                                                                   gcgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      <  1:5238976/93‑1 (MQ=255)

                                                                                   ccgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAt      <  1:5698853/92‑1 (MQ=255)

                                                                                    cgcCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTAtt     <  1:1419166/93‑1 (MQ=255)

                                                                                      ccAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTgg   <  1:15330/93‑1 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  <  2:4178671/93‑1 (MQ=255)

                                                                                       cAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTggg  >  1:4887738/1‑93 (MQ=255)

GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG  >  NC_000913/3279107‑3279286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279105‑3279290

GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              <  SRR4302532.3061266/94‑1 (MQ=60)

GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              >  SRR4302532.5294108/1‑94 (MQ=60)

GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCG                                                                                              <  SRR4302532.2074989/94‑1 (MQ=60)

 CGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGT                                                                                             >  SRR4302532.3998874/1‑94 (MQ=60)

  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302532.4555093/1‑94 (MQ=60)

  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302532.4193433/1‑94 (MQ=60)

  GGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTC                                                                                            >  SRR4302532.4092958/1‑94 (MQ=60)

  GGAAGCCATCCATAGGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTCGGATCGCTTAATCCGGGCAAACGCGCCAGCGTC                                                                                            <  SRR4302532.2704891/94‑1 (MQ=60)

   GAAGCCATCCATATGGCTGCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCG                                                                                           <  SRR4302532.1482061/94‑1 (MQ=60)

   GAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCG                                                                                           <  SRR4302532.6241176/94‑1 (MQ=60)

     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         >  SRR4302532.3370695/1‑94 (MQ=60)

     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         >  SRR4302532.5625546/1‑94 (MQ=60)

     AGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTT                                                                                         <  SRR4302532.1892164/94‑1 (MQ=60)

      GCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        <  SRR4302532.547704/94‑1 (MQ=60)

      ACCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTG                                                                                        <  SRR4302532.5225203/94‑1 (MQ=60)

        CATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCG                                                                                      >  SRR4302532.3041159/1‑94 (MQ=60)

         ATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGC                                                                                     >  SRR4302532.4947081/1‑94 (MQ=60)

          TGCATATGGCGTCGCTGCATTCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302532.3509712/94‑1 (MQ=60)

          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    >  SRR4302532.3555589/1‑94 (MQ=60)

          TCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302532.3509711/94‑1 (MQ=60)

          TCCATATGGCGTCGCTGCATCCGACGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCT                                                                                    <  SRR4302532.4444464/94‑1 (MQ=60)

            CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  <  SRR4302532.2012014/94‑1 (MQ=60)

            CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  <  SRR4302532.5760437/94‑1 (MQ=60)

            CATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGG                                                                                  >  SRR4302532.1177006/1‑94 (MQ=60)

             ATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGA                                                                                 >  SRR4302532.4951215/1‑94 (MQ=60)

              TATGGCGTCGGTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGTATCGCTTAAACCGGGCAACCGCGCCAGCGTCGTTGCGCTGGAT                                                                                <  SRR4302532.1474551/94‑1 (MQ=60)

                TGGCGTCGCTGCGTCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302532.3006074/94‑1 (MQ=60)

                TGGCGTCGCTGCATCCGGCGCGAATGCTGGTTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAG                                                                              <  SRR4302532.1279529/94‑1 (MQ=60)

                 GGCGTCGCTGCATCCGGCGCGAATGTTGGGTGTTGATGGTTTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302532.4745144/94‑1 (MQ=60)

                 GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             >  SRR4302532.1171465/1‑94 (MQ=60)

                 GGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGC                                                                             <  SRR4302532.2992286/94‑1 (MQ=60)

                  GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302532.3184412/94‑1 (MQ=60)

                  GCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCG                                                                            <  SRR4302532.2616583/94‑1 (MQ=60)

                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302532.2349707/94‑1 (MQ=60)

                   CGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302532.2790922/94‑1 (MQ=60)

                   CGGCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGG                                                                           <  SRR4302532.4945666/94‑1 (MQ=60)

                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          <  SRR4302532.3233322/94‑1 (MQ=60)

                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          <  SRR4302532.3086749/94‑1 (MQ=60)

                    GTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGG                                                                          >  SRR4302532.4846796/1‑94 (MQ=60)

                     TCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGC                                                                         >  SRR4302532.1494867/1‑94 (MQ=60)

                     TCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGC                                                                         >  SRR4302532.1494866/1‑94 (MQ=60)

                      CGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCT                                                                        <  SRR4302532.3223416/94‑1 (MQ=60)

                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302532.2109906/1‑94 (MQ=60)

                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302532.5020012/1‑94 (MQ=60)

                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       <  SRR4302532.697521/94‑1 (MQ=60)

                       GCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302532.3950841/1‑94 (MQ=60)

                       GCTGCATCCGGAGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTA                                                                       >  SRR4302532.1816341/1‑94 (MQ=60)

                        CTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTAC                                                                      >  SRR4302532.2034790/1‑94 (MQ=60)

                         TGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACA                                                                     <  SRR4302532.633348/94‑1 (MQ=60)

                         TGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACA                                                                     <  SRR4302532.633349/94‑1 (MQ=60)

                          GCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACAT                                                                    <  SRR4302532.943271/94‑1 (MQ=60)

                           CATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATG                                                                   <  SRR4302532.3724755/94‑1 (MQ=60)

                            ATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGT                                                                  >  SRR4302532.5052825/1‑94 (MQ=60)

                              CCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGC                                                                <  SRR4302532.1934335/94‑1 (MQ=60)

                              CCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGC                                                                <  SRR4302532.3658210/94‑1 (MQ=60)

                               CGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCA                                                               <  SRR4302532.6115422/94‑1 (MQ=60)

                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCTGGCTACATGTGCAAC                                                             <  SRR4302532.4486513/94‑1 (MQ=60)

                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302532.323519/94‑1 (MQ=60)

                                 GCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGATAGATAGCGGGCTACATGTGCAAC                                                             <  SRR4302532.5738432/94‑1 (MQ=60)

                                  CGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACA                                                            <  SRR4302532.5575221/94‑1 (MQ=60)

                                   GCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAA                                                           >  SRR4302532.4686789/1‑94 (MQ=60)

                                    CGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAA                                                          >  SRR4302532.5115925/1‑94 (MQ=60)

                                     GAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAAT                                                         <  SRR4302532.5910961/94‑1 (MQ=60)

                                      AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302532.1229318/1‑94 (MQ=60)

                                      AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302532.2543697/1‑94 (MQ=60)

                                      AATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATC                                                        >  SRR4302532.2543696/1‑94 (MQ=60)

                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       <  SRR4302532.2815439/94‑1 (MQ=60)

                                       ATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCT                                                       >  SRR4302532.5976380/1‑94 (MQ=60)

                                        TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302532.4212935/94‑1 (MQ=60)

                                        TGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTG                                                      <  SRR4302532.519736/94‑1 (MQ=60)

                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     >  SRR4302532.1315813/1‑94 (MQ=60)

                                         GCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGG                                                     >  SRR4302532.1941584/1‑94 (MQ=60)

                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302532.4328587/1‑94 (MQ=60)

                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    <  SRR4302532.6037075/94‑1 (MQ=60)

                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    >  SRR4302532.4994010/1‑94 (MQ=60)

                                          CTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGA                                                    <  SRR4302532.521892/94‑1 (MQ=60)

                                           TGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGAT                                                   <  SRR4302532.407329/94‑1 (MQ=60)

                                             GGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTC                                                 >  SRR4302532.6301009/1‑94 (MQ=60)

                                               TGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAG                                               >  SRR4302532.481126/1‑94 (MQ=60)

                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302532.3945298/94‑1 (MQ=60)

                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302532.6247801/94‑1 (MQ=60)

                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              >  SRR4302532.6349870/1‑94 (MQ=60)

                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302532.3616359/94‑1 (MQ=60)

                                                GTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGG                                              <  SRR4302532.661369/94‑1 (MQ=60)

                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302532.6300915/94‑1 (MQ=60)

                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             <  SRR4302532.4981300/94‑1 (MQ=60)

                                                 TTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGG                                             >  SRR4302532.974021/1‑94 (MQ=60)

                                                  TGATGGTGTTCTGGGATCGCTTAAGCCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            <  SRR4302532.4531689/94‑1 (MQ=60)

                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGT                                            >  SRR4302532.3407411/1‑94 (MQ=60)

                                                  TGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGC                                            >  SRR4302532.3355943/1‑94 (MQ=60)

                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           <  SRR4302532.107473/94‑1 (MQ=60)

                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           >  SRR4302532.57871/1‑94 (MQ=60)

                                                   GATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTC                                           <  SRR4302532.3001238/94‑1 (MQ=60)

                                                     TGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAA                                         >  SRR4302532.3509695/1‑94 (MQ=60)

                                                     TGGTGTTCTGGGACCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAA                                         >  SRR4302532.3509694/1‑94 (MQ=60)

                                                      GGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAAT                                        <  SRR4302532.4907205/94‑1 (MQ=60)

                                                       GTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATT                                       <  SRR4302532.2874541/94‑1 (MQ=60)

                                                         TTTCTGGGATCGCTTAAACCGGGCAGACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     <  SRR4302532.455752/94‑1 (MQ=60)

                                                         GTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAG                                     >  SRR4302532.6013917/1‑94 (MQ=60)

                                                          TTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGCGCAACAAATCTGGATTCAGGGTCAATTAGC                                    >  SRR4302532.2008950/1‑94 (MQ=60)

                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302532.324108/94‑1 (MQ=60)

                                                            CTGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTT                                  <  SRR4302532.6142357/94‑1 (MQ=60)

                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 >  SRR4302532.1650416/1‑94 (MQ=60)

                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302532.3029770/94‑1 (MQ=60)

                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 <  SRR4302532.5369094/94‑1 (MQ=60)

                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 >  SRR4302532.5431179/1‑94 (MQ=60)

                                                             TGGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTC                                 >  SRR4302532.702810/1‑94 (MQ=60)

                                                              GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302532.2527004/94‑1 (MQ=60)

                                                              GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302532.2527006/94‑1 (MQ=60)

                                                              GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302532.3937438/94‑1 (MQ=60)

                                                              GGGATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCG                                <  SRR4302532.6135572/94‑1 (MQ=60)

                                                                GATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTT                              >  SRR4302532.4450489/1‑94 (MQ=60)

                                                                 GTCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGGTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTT                             <  SRR4302532.4089099/94‑1 (MQ=60)

                                                                 ATCGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTT                             <  SRR4302532.4078285/94‑1 (MQ=60)

                                                                 ATCGCTTAAACAGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTT                             <  SRR4302532.2981675/94‑1 (MQ=60)

                                                                   CGCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTG                           <  SRR4302532.4118703/94‑1 (MQ=60)

                                                                    GCTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGA                          <  SRR4302532.3717979/94‑1 (MQ=60)

                                                                     CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         >  SRR4302532.3404054/1‑94 (MQ=60)

                                                                     CTTAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGAT                         <  SRR4302532.5794276/94‑1 (MQ=60)

                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302532.2333577/94‑1 (MQ=60)

                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       >  SRR4302532.1548043/1‑94 (MQ=60)

                                                                       TAAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAG                       <  SRR4302532.4676918/94‑1 (MQ=60)

                                                                        AAACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGT                      <  SRR4302532.1992843/94‑1 (MQ=60)

                                                                         AACCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTT                     <  SRR4302532.2097268/94‑1 (MQ=60)

                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   <  SRR4302532.1103747/94‑1 (MQ=60)

                                                                           CCGGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTG                   <  SRR4302532.520427/94‑1 (MQ=60)

                                                                             GGGCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCT                 >  SRR4302532.2437681/1‑94 (MQ=60)

                                                                               GCAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCC               >  SRR4302532.2466206/1‑94 (MQ=60)

                                                                                CAAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCT              >  SRR4302532.6209686/1‑94 (MQ=60)

                                                                                 AAACGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTT             <  SRR4302532.4704686/94‑1 (MQ=60)

                                                                                  ACCGCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTT            <  SRR4302532.2720168/94‑1 (MQ=60)

                                                                                     GCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         <  SRR4302532.5419606/94‑1 (MQ=60)

                                                                                     GCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         <  SRR4302532.1584433/94‑1 (MQ=60)

                                                                                     CCGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATT         <  SRR4302532.5897633/94‑1 (MQ=60)

                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302532.5580116/94‑1 (MQ=60)

                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302532.1521085/94‑1 (MQ=60)

                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302532.1466793/94‑1 (MQ=60)

                                                                                      CGCCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTG        <  SRR4302532.1466792/94‑1 (MQ=60)

                                                                                        CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      <  SRR4302532.15953/94‑1 (MQ=60)

                                                                                        CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302532.5055724/1‑94 (MQ=60)

                                                                                        CCAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGG      >  SRR4302532.3842706/1‑94 (MQ=60)

                                                                                         CAGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGC     <  SRR4302532.3576601/94‑1 (MQ=60)

                                                                                          AGCGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCC    >  SRR4302532.561406/1‑94 (MQ=60)

                                                                                            CGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  <  SRR4302532.702033/94‑1 (MQ=60)

GCGGAAGCCATCCATATGGCGTCGCTGCATCCGGCGCGAATGCTGGGTGTTGATGGTGTTCTGGGATCGCTTAAACCGGGCAAACGCGCCAGAGTCGTTGCGCTGGATAGCGGGCTACATGTGCAACAAATCTGGATTCAGGGTCAATTAGCTTCGTTTTGATAGTTTGCTCCTTTATTGGGCCTT  >  NC_000913/3279105‑3279290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`