Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,875	T→G	T61P (ACC→CCC)	sspA ←	stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,875	0	T	G	99.6%	796.0 / ‑5.8	223	T61P (ACC→CCC)	sspA	stringent starvation protein A
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (117/105); total (117/106)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 232 total aligned reads displayed.

CAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT  >  NC_000913/3374785‑3374964

cAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCAAAGGGTCAGCTCACGATCCACCAGGGGCg                                                                                         >  2:511069/1‑93 (MQ=255)

cAGGCATCAGTGGCGGATGCGGGAAACACTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCg                                                                                         <  2:1394917/93‑1 (MQ=255)

 aGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCgg                                                                                        >  1:1450245/1‑93 (MQ=255)

   gCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGaa                                                                                      >  2:1075163/1‑93 (MQ=255)

     aTCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACg                                                                                    >  2:4773702/1‑93 (MQ=255)

      tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGc                                                                                   >  1:4596604/1‑93 (MQ=255)

      tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGc                                                                                   >  1:4047324/1‑93 (MQ=255)

      tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGc                                                                                   >  2:2428664/1‑93 (MQ=255)

      tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGc                                                                                   >  2:2904307/1‑93 (MQ=255)

       cAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGct                                                                                  >  1:3141989/1‑93 (MQ=255)

        aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGctc                                                                                 <  1:3208815/93‑1 (MQ=255)

           ggCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGACCGCTCTGa                                                                              >  1:4360597/1‑93 (MQ=255)

           ggCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGa                                                                              <  1:546738/93‑1 (MQ=255)

            gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAt                                                                             >  2:1458701/1‑93 (MQ=255)

            gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAt                                                                             <  1:287577/93‑1 (MQ=255)

            gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAt                                                                             >  1:3068186/1‑93 (MQ=255)

              ggATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTc                                                                           <  2:4470201/93‑1 (MQ=255)

              ggATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTc                                                                           >  1:5296869/1‑93 (MQ=255)

               gATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCg                                                                          >  1:859654/1‑93 (MQ=255)

                aTGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCgg                                                                         <  2:3043988/93‑1 (MQ=255)

                aTGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCgg                                                                         <  1:2608869/93‑1 (MQ=255)

                aTGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCgg                                                                         <  1:2037589/93‑1 (MQ=255)

                 tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCggg                                                                        <  1:2653332/93‑1 (MQ=255)

                 tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCggg                                                                        <  1:189376/93‑1 (MQ=255)

                  gCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGt                                                                       <  1:778531/93‑1 (MQ=255)

                   cGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGtt                                                                      >  2:2084276/1‑93 (MQ=255)

                     ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGa                                                                    >  2:4267141/1‑93 (MQ=255)

                     ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGa                                                                    <  1:1828388/93‑1 (MQ=255)

                      gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   <  2:4636708/93‑1 (MQ=255)

                      gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   <  1:4204181/93‑1 (MQ=255)

                      gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   <  1:4426208/93‑1 (MQ=255)

                      gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   >  2:4884346/1‑93 (MQ=255)

                       aaaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAgg                                                                  <  2:465911/93‑1 (MQ=255)

                       aaaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAgg                                                                  >  2:817999/1‑93 (MQ=255)

                        aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGt                                                                 <  1:4248393/93‑1 (MQ=255)

                            cTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAt                                                             <  1:4944600/93‑1 (MQ=255)

                             tCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCCCGCTCCACCAGGGGCGGAACGCTCGGATTCGGGTTGAGGTCaaca                                                            >  2:4680512/1‑91 (MQ=255)

                              cATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCa                                                           <  1:3928862/93‑1 (MQ=255)

                              cATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCa                                                           >  1:5918420/1‑93 (MQ=255)

                               aTCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCATCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAg                                                          >  2:2342834/1‑93 (MQ=255)

                                tCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGa                                                         <  1:5266633/93‑1 (MQ=255)

                                tCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGa                                                         >  1:2342180/1‑93 (MQ=255)

                                 ccAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGAt                                                        <  2:1396822/93‑1 (MQ=255)

                                  cAGATATTCCATACTGATGCGAGATTCCCACAGGGTCAGCCCGCGATCCACCAGGGGCGGAACGCTCTGATTCGGGTGGAGGTCAATCAGATc                                                       <  1:5683589/93‑1 (MQ=255)

                                   aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGagcg                                                      <  1:1039684/93‑4 (MQ=255)

                                   aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      <  1:3008087/93‑1 (MQ=255)

                                   aGACATTCCATAATGATGCGAGATGCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      <  1:4554612/93‑1 (MQ=255)

                                    gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     >  1:618702/1‑93 (MQ=255)

                                    gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     <  2:196980/93‑1 (MQ=255)

                                    gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     >  1:3631844/1‑93 (MQ=255)

                                         tCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGc                                                <  2:2285703/93‑1 (MQ=255)

                                           cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                              >  2:2582301/1‑93 (MQ=255)

                                            aTAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGa                                             <  1:5036303/93‑1 (MQ=255)

                                             tAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAt                                            <  1:5838682/93‑1 (MQ=255)

                                              aaTGATGCGAGATTCCCACAGGGTCAGCGCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                                           <  1:1527600/93‑1 (MQ=255)

                                                tgatgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGt                                         >  2:5768669/1‑93 (MQ=255)

                                                   tgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCt                                      <  1:3563601/93‑1 (MQ=255)

                                                   tgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCt                                      <  1:4682859/93‑1 (MQ=255)

                                                    gCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCtt                                     <  2:1729667/93‑1 (MQ=255)

                                                     cGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCttt                                    <  1:4169127/93‑1 (MQ=255)

                                                      gagaTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGGTGCGGTCAATCAGATCCTGAGGCGGATTGTCCtttt                                   >  2:3250202/1‑93 (MQ=255)

                                                       agaTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTc                                  <  2:4132546/93‑1 (MQ=255)

                                                         aTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                                <  1:1656520/93‑1 (MQ=255)

                                                           tCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACg                              <  2:718649/93‑1 (MQ=255)

                                                            cccACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgt                             <  2:5637978/93‑1 (MQ=255)

                                                            cccACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgt                             >  2:6059573/1‑93 (MQ=255)

                                                             ccACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtg                            >  1:3858779/1‑93 (MQ=255)

                                                             ccACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtg                            >  2:3857157/1‑93 (MQ=255)

                                                               acaGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                          <  1:1979375/93‑1 (MQ=255)

                                                               acaGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                          >  2:1170291/1‑93 (MQ=255)

                                                               acaGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                          >  2:4052314/1‑93 (MQ=255)

                                                                caGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTc                         <  1:1104174/93‑1 (MQ=255)

                                                                  gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       >  2:5238136/1‑93 (MQ=255)

                                                                  gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       <  1:2099864/93‑1 (MQ=255)

                                                                  gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       >  1:977600/1‑93 (MQ=255)

                                                                  gggTCAGCGCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       <  1:3122552/93‑1 (MQ=255)

                                                                    gTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtc                     <  1:728543/93‑1 (MQ=255)

                                                                    gTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtc                     <  1:4315216/93‑1 (MQ=255)

                                                                     gcAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtct                    <  1:1546889/92‑1 (MQ=255)

                                                                      cAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtctc                   <  2:5949991/93‑1 (MQ=255)

                                                                          tCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGaaa               >  1:2244230/1‑93 (MQ=255)

                                                                           tccGAACCCCCCGGGGCGGAACGCCTTGGTTCCGGTTTAAGTCAAACAGAACCTTGGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAc              <  1:249467/91‑1 (MQ=255)

                                                                           cccGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAc              <  1:4855663/91‑1 (MQ=255)

                                                                           cACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAc              >  2:3002631/1‑93 (MQ=255)

                                                                            aCGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGGCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCCATCTCGAAACt             >  1:2616880/1‑93 (MQ=255)

                                                                              gATCCACCAGGGGCGGGACGCTCTGATTCGGGCTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTCTTCGATCTCGAAACttc           >  1:5911213/1‑92 (MQ=255)

                                                                              gATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTa           >  2:3479825/1‑93 (MQ=255)

                                                                              gATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTa           <  2:4914893/93‑1 (MQ=255)

                                                                               aTCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTac          >  1:478791/1‑93 (MQ=255)

                                                                                tCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTaca         <  2:5372940/93‑1 (MQ=255)

                                                                                 ccaccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTacac        >  2:283562/1‑93 (MQ=255)

                                                                                  caccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACAcc       <  1:1155291/93‑1 (MQ=255)

                                                                                   cccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCt      <  2:1908267/92‑1 (MQ=255)

                                                                                   cccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCt      <  2:3917166/92‑1 (MQ=255)

                                                                                   accaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCt      >  1:5792195/1‑93 (MQ=255)

                                                                                   accaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCCATCTCGACACTTACACCt      >  1:3343582/1‑93 (MQ=255)

                                                                                    ccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCtt     <  2:1300409/93‑1 (MQ=255)

                                                                                    ccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCtt     <  1:175299/93‑1 (MQ=255)

                                                                                    ccaGGGGCGGAACGCTCTGATGCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCtt     <  1:3656987/93‑1 (MQ=255)

                                                                                       ggggCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtct  <  1:956367/93‑1 (MQ=255)

CAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT  >  NC_000913/3374785‑3374964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  >  NC_000913/3374782‑3374967

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGG                                                                                              >  SRR4302532.4800585/1‑94 (MQ=60)

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGG                                                                                              >  SRR4302532.2488859/1‑94 (MQ=60)

 AACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATTCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGC                                                                                             <  SRR4302532.4426827/94‑1 (MQ=60)

   CAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGG                                                                                           >  SRR4302532.582254/1‑94 (MQ=60)

   CAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGG                                                                                           >  SRR4302532.1499141/1‑94 (MQ=60)

    AGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGAGTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGA                                                                                          <  SRR4302532.1679415/94‑1 (MQ=60)

     GGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAC                                                                                         >  SRR4302532.1201219/1‑94 (MQ=60)

        ATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGC                                                                                      >  SRR4302532.4183568/1‑94 (MQ=60)

        ATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGC                                                                                      >  SRR4302532.4197877/1‑94 (MQ=60)

        ATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGC                                                                                      >  SRR4302532.4753304/1‑94 (MQ=60)

         TCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCT                                                                                     >  SRR4302532.3246263/1‑94 (MQ=60)

         TCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCT                                                                                     <  SRR4302532.1790543/94‑1 (MQ=60)

           AGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCT                                                                                   <  SRR4302532.3315363/94‑1 (MQ=60)

             TGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGACCGCTCTGA                                                                                 >  SRR4302532.4508367/1‑94 (MQ=60)

              GGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAT                                                                                >  SRR4302532.3169569/1‑94 (MQ=60)

              GGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAT                                                                                <  SRR4302532.565781/94‑1 (MQ=60)

               GCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATT                                                                               <  SRR4302532.297499/94‑1 (MQ=60)

                CGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTC                                                                              >  SRR4302532.5479812/1‑94 (MQ=60)

                 GGTTGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCG                                                                             >  SRR4302532.5653064/1‑94 (MQ=60)

                 GGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGCACGCTCTGATTCG                                                                             >  SRR4302532.270542/1‑94 (MQ=60)

                 GGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCG                                                                             >  SRR4302532.889185/1‑94 (MQ=60)

                  GATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGG                                                                            <  SRR4302532.3767775/94‑1 (MQ=60)

                  GATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGG                                                                            >  SRR4302532.5096035/1‑94 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           <  SRR4302532.2696119/94‑1 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           <  SRR4302532.1520735/94‑1 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           <  SRR4302532.2105461/94‑1 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           <  SRR4302532.1580341/94‑1 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           >  SRR4302532.760263/1‑94 (MQ=60)

                    TGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGT                                                                          <  SRR4302532.2742384/94‑1 (MQ=60)

                    TGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGT                                                                          <  SRR4302532.195753/94‑1 (MQ=60)

                     GCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTT                                                                         <  SRR4302532.805326/94‑1 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      <  SRR4302532.1889243/94‑1 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      >  SRR4302532.2172809/1‑94 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      <  SRR4302532.3170820/94‑1 (MQ=60)

                         GAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGG                                                                     <  SRR4302532.4576990/94‑1 (MQ=60)

                         GAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGG                                                                     <  SRR4302532.4345331/94‑1 (MQ=60)

                         GAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGG                                                                     <  SRR4302532.561841/94‑1 (MQ=60)

                          AAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGT                                                                    <  SRR4302532.2592217/94‑1 (MQ=60)

                           AACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTC                                                                   <  SRR4302532.4391134/94‑1 (MQ=60)

                           AACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTC                                                                   >  SRR4302532.4647543/1‑94 (MQ=60)

                              GCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAT                                                                >  SRR4302532.6123286/1‑94 (MQ=60)

                              GCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAT                                                                <  SRR4302532.1500846/94‑1 (MQ=60)

                              GCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAT                                                                >  SRR4302532.732582/1‑94 (MQ=60)

                              GCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAT                                                                >  SRR4302532.5019478/1‑94 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTTACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               >  SRR4302532.6339460/1‑94 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               <  SRR4302532.5114313/94‑1 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               >  SRR4302532.2800286/1‑94 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               >  SRR4302532.2370120/1‑94 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               >  SRR4302532.1951147/1‑94 (MQ=60)

                                TCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCCCCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCA                                                              <  SRR4302532.4340119/94‑1 (MQ=60)

                                TCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCA                                                              >  SRR4302532.6123386/1‑94 (MQ=60)

                                TCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCA                                                              <  SRR4302532.2064908/94‑1 (MQ=60)

                                 CATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAG                                                             <  SRR4302532.4061237/94‑1 (MQ=60)

                                 CATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAG                                                             >  SRR4302532.5928648/1‑94 (MQ=60)

                                  ATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGA                                                            >  SRR4302532.2420251/1‑94 (MQ=60)

                                   TCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGAT                                                           <  SRR4302532.524218/94‑1 (MQ=60)

                                   TCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGAT                                                           <  SRR4302532.5448244/94‑1 (MQ=60)

                                    CCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATC                                                          >  SRR4302532.3901636/1‑94 (MQ=60)

                                    CCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATC                                                          >  SRR4302532.3493752/1‑94 (MQ=60)

                                     CAGATATTCCATACTGATGCGAGATTCCCACAGGGTCAGCCCGCGATCCACCAGGGGCGGAACGCTCTGATTCGGGTGGAGGTCAATCAGATCC                                                         <  SRR4302532.5881993/94‑1 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGAgcgt                                                        <  SRR4302532.1074749/94‑5 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        >  SRR4302532.3753667/1‑94 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        >  SRR4302532.640266/1‑94 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        >  SRR4302532.3423814/1‑94 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        <  SRR4302532.3107389/94‑1 (MQ=60)

                                      AGACATTCCATAATGATGCGAGATGCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        <  SRR4302532.4710023/94‑1 (MQ=60)

                                       GATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTG                                                       <  SRR4302532.4854296/94‑1 (MQ=60)

                                        ATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGA                                                      >  SRR4302532.3231848/1‑94 (MQ=60)

                                         TATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAG                                                     <  SRR4302532.46077/94‑1 (MQ=60)

                                          ATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGG                                                    <  SRR4302532.453851/94‑1 (MQ=60)

                                          ATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGG                                                    <  SRR4302532.6083823/94‑1 (MQ=60)

                                            TCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCG                                                  <  SRR4302532.4413542/94‑1 (MQ=60)

                                             CCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATGAGATCCTGAGGCGG                                                 >  SRR4302532.1155154/1‑94 (MQ=60)

                                              CATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGA                                                <  SRR4302532.6005869/94‑1 (MQ=60)

                                              CATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGA                                                <  SRR4302532.996172/94‑1 (MQ=60)

                                               ATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAT                                               <  SRR4302532.5209034/94‑1 (MQ=60)

                                               ATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAT                                               <  SRR4302532.3060173/94‑1 (MQ=60)

                                               ATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAT                                               <  SRR4302532.3142440/94‑1 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              <  SRR4302532.6041331/94‑1 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              >  SRR4302532.1822105/1‑94 (MQ=60)

                                                 AATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTG                                             <  SRR4302532.3911286/94‑1 (MQ=60)

                                                 AATGATGCGAGATTCCCACAGGGTCAGCGCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTG                                             <  SRR4302532.1579206/94‑1 (MQ=60)

                                                  ATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGT                                            >  SRR4302532.5175543/1‑94 (MQ=60)

                                                  ATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGT                                            >  SRR4302532.1983775/1‑94 (MQ=60)

                                                  ATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGT                                            >  SRR4302532.320920/1‑94 (MQ=60)

                                                   TGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTC                                           >  SRR4302532.3295590/1‑94 (MQ=60)

                                                    GATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCC                                          <  SRR4302532.4710920/94‑1 (MQ=60)

                                                     ATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCT                                         >  SRR4302532.3688968/1‑94 (MQ=60)

                                                      TGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTT                                        <  SRR4302532.4842560/94‑1 (MQ=60)

                                                      TGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTT                                        <  SRR4302532.3682833/94‑1 (MQ=60)

                                                        CGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTT                                      <  SRR4302532.4308952/94‑1 (MQ=60)

                                                         GAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTC                                     >  SRR4302532.4914213/1‑94 (MQ=60)

                                                         GAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTC                                     <  SRR4302532.926040/94‑1 (MQ=60)

                                                          AGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCC                                    >  SRR4302532.2169036/1‑94 (MQ=60)

                                                           GATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGACCGCTCTGATTCGGGTGGAGGTGAATCAGATCCTGAGGCGGATTGTCCTTTTCCA                                   <  SRR4302532.4375836/94‑1 (MQ=60)

                                                            ATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAC                                  <  SRR4302532.1712661/94‑1 (MQ=60)

                                                              TCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGT                                <  SRR4302532.404580/94‑1 (MQ=60)

                                                              TCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGT                                <  SRR4302532.4456821/94‑1 (MQ=60)

                                                               CCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTG                               >  SRR4302532.3989060/1‑94 (MQ=60)

                                                                CCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGT                              <  SRR4302532.899711/94‑1 (MQ=60)

                                                                  ACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTC                            <  SRR4302532.2045131/94‑1 (MQ=60)

                                                                  ACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTC                            <  SRR4302532.2269484/94‑1 (MQ=60)

                                                                   CAGGGTCAGCTCGCGATCCACCAGGGGCGCAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGGGTTCG                           <  SRR4302532.1075294/94‑1 (MQ=60)

                                                                   CAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCG                           <  SRR4302532.1141085/94‑1 (MQ=60)

                                                                   CAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCG                           >  SRR4302532.2709960/1‑94 (MQ=60)

                                                                    AGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGA                          >  SRR4302532.1010965/1‑94 (MQ=60)

                                                                     GGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAT                         <  SRR4302532.2169922/94‑1 (MQ=60)

                                                                     GGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAT                         >  SRR4302532.868006/1‑94 (MQ=60)

                                                                     GGGTCAGCGCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAT                         <  SRR4302532.3226182/94‑1 (MQ=60)

                                                                      GGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATC                        <  SRR4302532.5471123/94‑1 (MQ=60)

                                                                       GTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCT                       <  SRR4302532.753370/94‑1 (MQ=60)

                                                                       GTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCT                       >  SRR4302532.3442811/1‑94 (MQ=60)

                                                                       GTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCT                       <  SRR4302532.4461106/94‑1 (MQ=60)

                                                                        GCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTC                      <  SRR4302532.1599156/94‑1 (MQ=60)

                                                                          AGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGA                    >  SRR4302532.2605431/1‑94 (MQ=60)

                                                                            CTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAA                  >  SRR4302532.2318713/1‑94 (MQ=60)

                                                                             TCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAC                 <  SRR4302532.1990222/94‑1 (MQ=60)

                                                                              CCCGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACT                <  SRR4302532.5022736/94‑1 (MQ=60)

                                                                              CACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACT                >  SRR4302532.475959/1‑94 (MQ=60)

                                                                              CACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGGCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCCATCTCGAAACT                >  SRR4302532.2704456/1‑94 (MQ=60)

                                                                               ACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTT               <  SRR4302532.6006739/94‑1 (MQ=60)

                                                                                CGATCCACCAGGGGCGGGACGCTCTGATTCGGGCTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTCTTCGATCTCGAAACTTC              >  SRR4302532.6115933/1‑94 (MQ=60)

                                                                                 GATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTAC             >  SRR4302532.5179930/1‑94 (MQ=60)

                                                                                 GATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTAC             >  SRR4302532.2774465/1‑94 (MQ=60)

                                                                                 GATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTAC             >  SRR4302532.495484/1‑94 (MQ=60)

                                                                                  ATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACA            <  SRR4302532.1636753/94‑1 (MQ=60)

                                                                                     CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCT         <  SRR4302532.1811210/94‑1 (MQ=60)

                                                                                     CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCT         >  SRR4302532.5993559/1‑94 (MQ=60)

                                                                                     CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCT         <  SRR4302532.1193835/94‑1 (MQ=60)

                                                                                     CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCCATCTCGACACTTACACCT         >  SRR4302532.3455178/1‑94 (MQ=60)

                                                                                       CCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTT       <  SRR4302532.181169/94‑1 (MQ=60)

                                                                                       CCAGGGGCGGAACGCTCTGATGCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTT       <  SRR4302532.3779769/94‑1 (MQ=60)

                                                                                         AGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     <  SRR4302532.430649/94‑1 (MQ=60)

                                                                                         AGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     <  SRR4302532.66734/94‑1 (MQ=60)

                                                                                         AGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     <  SRR4302532.2426908/94‑1 (MQ=60)

                                                                                         AGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCATTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     >  SRR4302532.4503444/1‑94 (MQ=60)

                                                                                          GGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTC    >  SRR4302532.4194816/1‑94 (MQ=60)

                                                                                          GGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTC    <  SRR4302532.989023/94‑1 (MQ=60)

                                                                                          GGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTC    <  SRR4302532.4091912/94‑1 (MQ=60)

                                                                                            GGCGGAGCGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  <  SRR4302532.1257899/94‑1 (MQ=60)

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  >  NC_000913/3374782‑3374967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`