Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,840	+G	coding (1070/1089 nt)	b3269 →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,837	1	.	G	99.5%	713.3 / ‑4.5	202	T356R (ACG→AGG)	b3269	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base . (0/0); major base G (97/103); minor base A (1/0); total (98/104)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 231 total aligned reads displayed.

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAc                                                                                           <  1:1023882/93‑1 (MQ=255)

gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAc                                                                                           >  2:1125208/1‑93 (MQ=255)

gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAc                                                                                           <  1:3769253/93‑1 (MQ=255)

gTATCTCAACATCGTCAAAAACCCCTCTCTTGCCTCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAc                                                                                           <  2:4935491/93‑1 (MQ=255)

 tATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g                                                                                         >  2:2064918/1‑93 (MQ=255)

   tctcAACCTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑ggg                                                                                       <  2:3720308/93‑1 (MQ=255)

   tctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCTTGCTGAATCAGAC‑ggg                                                                                       >  1:26666/1‑93 (MQ=255)

   tctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑ggg                                                                                       >  2:5105170/1‑93 (MQ=255)

   tcccAACATCGTCAAAAACTCCTCCCTTGCCGCCGCTCTTGGCTATCCCGATATGGTTTCGCTGTTTGCAGGCACCGTGCTGAATCAGAC‑ggg                                                                                       <  1:5302431/90‑1 (MQ=255)

     tcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑ggggc                                                                                     <  1:688580/93‑3 (MQ=255)

       aaCATCGTCAAAAACTCCTCACTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑ggggcaa                                                                                   >  1:2563596/1‑89 (MQ=255)

         cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGGTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGc                                                                                 <  1:829397/93‑1 (MQ=255)

         cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGc                                                                                 >  2:5029843/1‑93 (MQ=255)

         cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGc                                                                                 <  1:2542921/93‑1 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGcc                                                                                >  1:3077291/1‑93 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGcc                                                                                >  1:3851808/1‑93 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGcc                                                                                >  2:5933650/1‑93 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGcc                                                                                >  2:3318361/1‑93 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGcc                                                                                >  2:4228830/1‑93 (MQ=255)

             gTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATc                                                                             >  1:2972075/1‑93 (MQ=255)

              tCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCg                                                                            >  2:1394141/1‑93 (MQ=255)

               cAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCga                                                                           <  2:906501/93‑1 (MQ=255)

                aaaacCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCgag                                                                          <  1:5022845/93‑1 (MQ=255)

                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCgaga                                                                         >  2:2160742/1‑93 (MQ=255)

                  aaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCACCGAGAc                                                                        >  2:1541905/1‑93 (MQ=255)

                   aaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACg                                                                       >  1:3331156/1‑93 (MQ=255)

                     ctcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGAt                                                                     <  2:5530277/93‑1 (MQ=255)

                     ctcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGAt                                                                     >  1:3253171/1‑93 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATa                                                                    >  2:4809248/1‑93 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATa                                                                    >  2:1200411/1‑93 (MQ=255)

                      ccctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATa                                                                    <  2:3915982/92‑1 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAg                                                                   >  1:4931549/1‑93 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGcc                                                                 <  2:4329410/93‑1 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGca                                                                 >  1:2638466/1‑92 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCa                                                                <  2:4022448/93‑1 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCa                                                                <  2:1464174/93‑1 (MQ=255)

                           tctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAt                                                               >  1:5747968/1‑93 (MQ=255)

                           tctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAt                                                               >  1:3411952/1‑93 (MQ=255)

                           cctggccgccGCTATTGGCTATCCCGAGATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAt                                                               <  2:5267030/89‑1 (MQ=255)

                             ttGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGa                                                             <  2:4275635/93‑1 (MQ=255)

                                ccgccgCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCa                                                          <  2:4543534/93‑1 (MQ=255)

                                 cgccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCAt                                                         >  1:5997858/1‑93 (MQ=255)

                                  gccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATg                                                        >  1:4754088/1‑93 (MQ=255)

                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       >  1:3963528/1‑93 (MQ=255)

                                    cgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTc                                                      <  2:4922513/93‑1 (MQ=255)

                                    cgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTc                                                      >  2:4036265/1‑93 (MQ=255)

                                    cgcTATTGGCGATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTc                                                      <  1:2071906/93‑1 (MQ=255)

                                     gcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCg                                                     >  2:3508091/1‑93 (MQ=255)

                                      cTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGACATGACCATGTCgg                                                    >  2:3658401/1‑93 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  >  2:5949223/1‑93 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  >  2:566925/1‑93 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  <  1:2806946/93‑1 (MQ=255)

                                         ttGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCt                                                 >  2:5630182/1‑93 (MQ=255)

                                         ttGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCt                                                 >  2:3366269/1‑93 (MQ=255)

                                          tGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTa                                                >  1:4403235/1‑93 (MQ=255)

                                          tGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTa                                                <  1:2251574/93‑1 (MQ=255)

                                           ggCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAt                                               <  2:3077291/93‑1 (MQ=255)

                                              tATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTg                                            >  1:1433030/1‑93 (MQ=255)

                                               aTCCCGATATGGTTTCGCTGTTTACCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGa                                           >  1:3726484/1‑93 (MQ=255)

                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAGGACCATGACGGTCTATCTGATTa                                        >  1:1922210/1‑93 (MQ=255)

                                                   cGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAt                                       <  1:2995545/93‑1 (MQ=255)

                                                     atatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCa                                     >  1:2004865/1‑93 (MQ=255)

                                                     atatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCa                                     >  2:2786176/1‑93 (MQ=255)

                                                     atatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCa                                     >  2:2808159/1‑93 (MQ=255)

                                                     atatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCa                                     >  2:3207149/1‑93 (MQ=255)

                                                      tatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGGCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCac                                    >  1:1241699/1‑92 (MQ=255)

                                                        tGGTTTCGCTGTTTGCCGCCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                  <  1:1185160/93‑1 (MQ=255)

                                                          gTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                >  2:3026013/1‑93 (MQ=255)

                                                          gTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                >  2:2603953/1‑93 (MQ=255)

                                                                cTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtc                          <  1:719123/93‑1 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         <  1:5130595/93‑1 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         >  1:1404631/1‑93 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         >  1:5608134/1‑93 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACAGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         >  2:773329/1‑93 (MQ=255)

                                                                  gTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                        >  1:1121646/1‑93 (MQ=255)

                                                                  gTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                        <  1:4467181/93‑1 (MQ=255)

                                                                    ttGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgc                      >  1:2168983/1‑93 (MQ=255)

                                                                      cccGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctg                    <  2:162259/92‑1 (MQ=255)

                                                                       ccGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgc                   <  2:6004358/93‑1 (MQ=255)

                                                                       ccGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgc                   <  1:583693/93‑1 (MQ=255)

                                                                          gCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                >  2:255298/1‑93 (MQ=255)

                                                                          gCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                <  1:31707/93‑1 (MQ=255)

                                                                           cACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgat               <  2:353117/93‑1 (MQ=255)

                                                                            cccGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatg              <  2:1192529/92‑1 (MQ=255)

                                                                            aCCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatg              >  2:2490057/1‑93 (MQ=255)

                                                                             ccGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatga             >  1:2725564/1‑93 (MQ=255)

                                                                              cGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGaa            <  1:1842348/93‑1 (MQ=255)

                                                                              cGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGaa            <  1:1552330/93‑1 (MQ=255)

                                                                                tGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAata          >  2:2372244/1‑93 (MQ=255)

                                                                                ggCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAata          <  1:897357/92‑1 (MQ=255)

                                                                                 gCTGAATCATAA‑GGGACGAGCCATCGAGACGATAGCAATGAACATGTCGGTCTATATGATTATCAGCCTGACTATCTCGCTGCTGATGCatgt         <  1:5498560/93‑3 (MQ=255)

                                                                                   tGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCt       >  1:5979690/1‑93 (MQ=255)

                                                                                    gAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCta      <  2:4260541/93‑1 (MQ=255)

                                                                                     aaTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat     <  1:4195728/93‑1 (MQ=255)

                                                                                     aaTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat     <  2:1064900/93‑1 (MQ=255)

                                                                                     aaTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat     <  2:435358/93‑1 (MQ=255)

                                                                                     aaTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat     <  2:724918/93‑1 (MQ=255)

                                                                                      aTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtata    <  2:5193534/93‑1 (MQ=255)

                                                                                      aTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtata    <  2:1758562/93‑1 (MQ=255)

                                                                                      aTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtata    <  2:3355152/93‑1 (MQ=255)

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  >  NC_000913/3418747‑3418933

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCTTGCTGAATCAGAC‑GGG                                                                                               >  SRR4302532.27714/1‑94 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG                                                                                               >  SRR4302532.2267416/1‑94 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG                                                                                               >  SRR4302532.1951871/1‑94 (MQ=60)

 TCCCAACATCGTCAAAAACTCCTCCCTTGCCGCCGCTCTTGGCTATCCCGATATGGTTTCGCTGTTTGCAGGCACCGTGCTGAATCAGAC‑GGGG                                                                                              <  SRR4302532.5485682/94‑1 (MQ=60)

   TCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGgca                                                                                            <  SRR4302532.712202/94‑4 (MQ=60)

    CAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAA                                                                                            >  SRR4302532.5976305/1‑94 (MQ=60)

    CAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAA                                                                                            <  SRR4302532.1465935/94‑1 (MQ=60)

    CAACATCGTCAAAAACTCCTCACTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAA                                                                                            >  SRR4302532.2649143/1‑94 (MQ=60)

     AACATCGTCAAAAACTGCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGCTTCGCTCTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAG                                                                                           >  SRR4302532.962705/1‑94 (MQ=60)

      ACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGC                                                                                          >  SRR4302532.3890711/1‑94 (MQ=60)

      ACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGC                                                                                          <  SRR4302532.4958342/94‑1 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGGTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCC                                                                                         <  SRR4302532.857781/94‑1 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCC                                                                                         <  SRR4302532.729920/94‑1 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCC                                                                                         >  SRR4302532.3981856/1‑94 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCC                                                                                         <  SRR4302532.857565/94‑1 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCC                                                                                         <  SRR4302532.2627733/94‑1 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCC                                                                                         >  SRR4302532.3178979/1‑94 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGgcaaccc                                                                                        <  SRR4302532.3709871/94‑8 (MQ=60)

          CGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATC                                                                                      >  SRR4302532.1790937/1‑94 (MQ=60)

          CGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATC                                                                                      >  SRR4302532.3070439/1‑94 (MQ=60)

           GTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCG                                                                                     <  SRR4302532.6155359/94‑1 (MQ=60)

             CAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCAGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAG                                                                                   <  SRR4302532.6293841/94‑1 (MQ=60)

              AAAACCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGA                                                                                  <  SRR4302532.5195122/94‑1 (MQ=60)

                AAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACG                                                                                >  SRR4302532.3442246/1‑94 (MQ=60)

                 AACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGGTTGCCGGCAACGTGCTGAATCAGACGGGGCAAGCCATCGAGACAA                                                                               >  SRR4302532.2109112/1‑94 (MQ=60)

                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGAT                                                                              >  SRR4302532.3361235/1‑94 (MQ=60)

                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGAT                                                                              >  SRR4302532.1529473/1‑94 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAG                                                                            >  SRR4302532.5100884/1‑94 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAG                                                                            >  SRR4302532.4179760/1‑94 (MQ=60)

                    CCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATTGAGACGATAG                                                                            <  SRR4302532.6196178/94‑1 (MQ=60)

                    CCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAG                                                                            <  SRR4302532.3078399/94‑1 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCA                                                                          >  SRR4302532.2726916/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGACAGCC                                                                          >  SRR4302532.4871488/1‑94 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCAT                                                                        >  SRR4302532.3526215/1‑94 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCAT                                                                        >  SRR4302532.1340044/1‑94 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCAT                                                                        >  SRR4302532.749686/1‑94 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCAT                                                                        >  SRR4302532.5948048/1‑94 (MQ=60)

                         TCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATG                                                                       <  SRR4302532.1866966/94‑1 (MQ=60)

                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGA                                                                      >  SRR4302532.3691154/1‑94 (MQ=60)

                           TTGCCGCCGCTAGTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGAC                                                                     <  SRR4302532.2926482/94‑1 (MQ=60)

                              CCGCCGCTATTGGCTATCCCGGTATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCAT                                                                  <  SRR4302532.938976/94‑1 (MQ=60)

                              CCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCAT                                                                  >  SRR4302532.6205029/1‑94 (MQ=60)

                              CCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCAT                                                                  >  SRR4302532.1176690/1‑94 (MQ=60)

                               CGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATG                                                                 >  SRR4302532.4916663/1‑94 (MQ=60)

                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                                >  SRR4302532.3144420/1‑94 (MQ=60)

                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                                >  SRR4302532.4097066/1‑94 (MQ=60)

                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                                <  SRR4302532.5754600/94‑1 (MQ=60)

                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTC                                                               >  SRR4302532.5586500/1‑94 (MQ=60)

                                  CGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCG                                                              <  SRR4302532.3570262/94‑1 (MQ=60)

                                  CGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCG                                                              <  SRR4302532.3277925/94‑1 (MQ=60)

                                  CGCTATTGGCGATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCG                                                              <  SRR4302532.2140968/94‑1 (MQ=60)

                                      ATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCT                                                          <  SRR4302532.2900958/94‑1 (MQ=60)

                                       TTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTA                                                         >  SRR4302532.4553158/1‑94 (MQ=60)

                                        TGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAT                                                        <  SRR4302532.2326308/94‑1 (MQ=60)

                                         GGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATC                                                       <  SRR4302532.516278/94‑1 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                      >  SRR4302532.1446125/1‑94 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                     >  SRR4302532.1481273/1‑94 (MQ=60)

                                            TATCCCGATATGGTTTCGCTGTTTACCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGA                                                    >  SRR4302532.3851647/1‑94 (MQ=60)

                                             ATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAT                                                   <  SRR4302532.3294640/94‑1 (MQ=60)

                                              TCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACTGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATT                                                  <  SRR4302532.5442197/94‑1 (MQ=60)

                                              TCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATT                                                  >  SRR4302532.4309258/1‑94 (MQ=60)

                                              TCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCAATGACCATGTCCGTCTATCTGATT                                                  >  SRR4302532.456892/1‑94 (MQ=60)

                                               CCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTA                                                 >  SRR4302532.873402/1‑94 (MQ=60)

                                               CCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCAGGACCATGACGGTCTATCTGATTA                                                 >  SRR4302532.1986145/1‑94 (MQ=60)

                                                CCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGGAAGCCATCCAGACGAAAGCCAAGAACATGCCGGGCTATCTGATTCT                                                >  SRR4302532.5796128/1‑94 (MQ=53)

                                                 CGATATGGTTTCGCTGTTTGCCTGCACCGTGCTGAATCACACGGGGCCAGCCATCGCGACGATAACCATGACCATGTCGTTCTATCTGATTATA                                               >  SRR4302532.5248186/1‑94 (MQ=60)

                                                 CGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATC                                               <  SRR4302532.3094513/94‑1 (MQ=60)

                                                  GATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCA                                              >  SRR4302532.2777707/1‑94 (MQ=60)

                                                  GATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCA                                              >  SRR4302532.2071703/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGGCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAC                                             >  SRR4302532.1283248/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                             >  SRR4302532.5888567/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGCGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGACATGACCATGTCGGTCTATCTGCTTATCAG                                             >  SRR4302532.2239897/1‑94 (MQ=60)

                                                    TATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGC                                            >  SRR4302532.3941243/1‑94 (MQ=60)

                                                     ATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCC                                           <  SRR4302532.2138135/94‑1 (MQ=60)

                                                      TGGTTTCGCTTTCTGCCGGCCCCGTGCTCAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          <  SRR4302532.683904/94‑1 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGCCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          <  SRR4302532.1224769/94‑1 (MQ=60)

                                                       GGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTG                                         <  SRR4302532.5251242/94‑1 (MQ=60)

                                                       GGTTCCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTG                                         <  SRR4302532.281600/94‑1 (MQ=60)

                                                         TTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAC                                       <  SRR4302532.3252133/94‑1 (MQ=60)

                                                          TTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                      >  SRR4302532.3492877/1‑94 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                    <  SRR4302532.5022440/94‑1 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                                  <  SRR4302532.743678/94‑1 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                                  >  SRR4302532.1451681/1‑94 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                                  >  SRR4302532.5802787/1‑94 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                 >  SRR4302532.1159100/1‑94 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                 >  SRR4302532.123771/1‑94 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                 <  SRR4302532.5307093/94‑1 (MQ=60)

                                                                GTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCG                                <  SRR4302532.4619486/94‑1 (MQ=60)

                                                                GTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCG                                <  SRR4302532.2633508/94‑1 (MQ=60)

                                                                 TTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGC                               >  SRR4302532.2241512/1‑94 (MQ=60)

                                                                 TTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGC                               <  SRR4302532.1113539/94‑1 (MQ=60)

                                                                     CCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCT                           <  SRR4302532.604049/94‑1 (MQ=60)

                                                                        GCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGAT                        <  SRR4302532.4876092/94‑1 (MQ=60)

                                                                        GCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGAT                        <  SRR4302532.32903/94‑1 (MQ=60)

                                                                         CACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATG                       >  SRR4302532.4075121/1‑94 (MQ=60)

                                                                         CACCGTGCTGAAGCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATG                       <  SRR4302532.3377118/94‑1 (MQ=60)

                                                                          CCCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGA                      <  SRR4302532.2518711/94‑1 (MQ=60)

                                                                          ACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGA                      >  SRR4302532.2817229/1‑94 (MQ=60)

                                                                            CGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAAT                    <  SRR4302532.1903543/94‑1 (MQ=60)

                                                                            CGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAAT                    <  SRR4302532.1604803/94‑1 (MQ=60)

                                                                             GTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                   <  SRR4302532.2692034/94‑1 (MQ=60)

                                                                             GTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                   <  SRR4302532.5198575/94‑1 (MQ=60)

                                                                              TGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT                  <  SRR4302532.3093642/94‑1 (MQ=60)

                                                                              TGCTGAACCAGACGGGGCAAGCCATCGAGACGATAGCAATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT                  <  SRR4302532.5737567/94‑1 (MQ=60)

                                                                              GGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT                  <  SRR4302532.927972/94‑1 (MQ=60)

                                                                               gctgaaacaagcgGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATC                 <  SRR4302532.1926376/81‑1 (MQ=60)

                                                                                CTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT                >  SRR4302532.6186332/1‑94 (MQ=60)

                                                                                CTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT                <  SRR4302532.2034710/94‑1 (MQ=60)

                                                                                   AATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATA             <  SRR4302532.6122352/94‑1 (MQ=60)

                                                                                   AATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATA             <  SRR4302532.4336545/94‑1 (MQ=60)

                                                                                   AATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATA             <  SRR4302532.4386848/94‑1 (MQ=60)

                                                                                    ATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAA            <  SRR4302532.2388995/94‑1 (MQ=60)

                                                                                     TCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC           <  SRR4302532.2858977/94‑1 (MQ=60)

                                                                                      CAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC          <  SRR4302532.2747961/94‑1 (MQ=60)

                                                                                      CAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC          >  SRR4302532.2280479/1‑94 (MQ=60)

                                                                                       AGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCG         >  SRR4302532.6253224/1‑94 (MQ=60)

                                                                                       AGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCG         >  SRR4302532.2533730/1‑94 (MQ=60)

                                                                                         acgGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCC       <  SRR4302532.601576/91‑1 (MQ=60)

                                                                                          cgGGGCACGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCG      <  SRR4302532.38364/92‑1 (MQ=60)

                                                                                             GGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAT   <  SRR4302532.3976768/94‑1 (MQ=60)

                                                                                              GCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  >  SRR4302532.4801550/1‑94 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  >  NC_000913/3418747‑3418933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`