Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,429,527 Δ1 bp coding (40/510 nt) yrdD ← putative DNA topoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,429,5260C.100.0% 1033.2 / NA 220coding (41/510 nt)yrdDputative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (120/100);  total (120/100)

Only 100 of 230 total aligned reads displayed.

CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614
                                                                                            |                                                                                        
     tGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATa                                                                                    >  2:5617299/1‑93 (MQ=255)
       tCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAc                                                                                  <  2:3387339/93‑1 (MQ=255)
       tCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAc                                                                                  >  2:4038281/1‑93 (MQ=255)
        ccATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAcc                                                                                 <  2:494739/93‑1 (MQ=255)
          aTCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg                                                                               >  1:1757822/1‑93 (MQ=255)
          aTCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg                                                                               >  1:6022813/1‑93 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTAAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              >  1:2098227/1‑93 (MQ=255)
              gCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa                                                                           <  1:4255018/93‑1 (MQ=255)
              gCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa                                                                           <  1:2113344/93‑1 (MQ=255)
               cTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAg                                                                          >  1:675606/1‑93 (MQ=255)
                tGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGc                                                                         >  2:4074957/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        >  2:4717662/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        >  1:2456893/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        <  2:115633/93‑1 (MQ=255)
                  aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGccc                                                                       <  1:505549/93‑1 (MQ=255)
                   aGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc                                                                      <  1:4714168/93‑1 (MQ=255)
                     aTTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGc                                                                    >  2:3075/1‑93 (MQ=255)
                      tttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCa                                                                   <  1:5163255/93‑1 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  >  1:3913937/1‑93 (MQ=255)
                        tCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACt                                                                 <  1:4669897/93‑1 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              >  2:3641388/1‑93 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              <  2:5643161/93‑1 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             <  1:3990900/93‑1 (MQ=255)
                             agacggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            <  1:5107668/92‑1 (MQ=255)
                              gacggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGc                                                           <  2:2970123/93‑1 (MQ=255)
                               acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa                                                          <  2:269542/93‑1 (MQ=255)
                               acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa                                                          <  2:5425443/93‑1 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      <  2:3083155/93‑1 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      <  2:3753960/93‑1 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      <  1:4759209/93‑1 (MQ=255)
                                     gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACta                                                    >  2:6149269/1‑92 (MQ=255)
                                     gTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTc                                                    >  2:349327/1‑93 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   >  2:3902350/1‑93 (MQ=255)
                                       aGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat                                                  <  1:947735/93‑1 (MQ=255)
                                       aGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat                                                  >  2:932707/1‑93 (MQ=255)
                                        gTCGCACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt                                                 <  1:5544968/93‑1 (MQ=255)
                                        gTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt                                                 >  1:3108357/1‑93 (MQ=255)
                                        gTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt                                                 <  2:4271566/93‑1 (MQ=255)
                                        gTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt                                                 <  2:4378666/93‑1 (MQ=255)
                                         tCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatta                                                >  1:1464122/1‑93 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              >  2:4179332/1‑93 (MQ=255)
                                            cacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta                                             <  1:4227527/93‑1 (MQ=255)
                                              cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACg                                           <  2:523174/93‑1 (MQ=255)
                                               gCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGc                                          >  2:2826407/1‑93 (MQ=255)
                                                ccGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCa                                         <  1:3745487/93‑1 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCACGCACTTTGGGCAGGACTCATTATTACGCAc                                        >  2:1720016/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       >  1:3463500/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       >  2:1482726/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  1:4693951/93‑1 (MQ=255)
                                                   gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg                                      >  1:5685872/1‑93 (MQ=255)
                                                    aTACTGTCAGCATCCAAGAAACGGACCGTGTTGCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt                                     <  1:2597950/93‑1 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    >  2:5115062/1‑93 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    >  1:1408785/1‑93 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    >  2:3868918/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:5767297/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTACCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:5383457/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                >  2:5567430/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                >  2:1016594/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:309168/93‑1 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:4825487/93‑1 (MQ=255)
                                                         ggaaGCAACCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:4885851/90‑1 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               <  2:3220003/93‑1 (MQ=255)
                                                           gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt                              <  1:1365667/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            <  2:4895857/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            <  1:1285340/93‑1 (MQ=255)
                                                               aTCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg                          >  1:372839/1‑93 (MQ=255)
                                                               aTCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg                          >  2:4026695/1‑93 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCCGGACTCATTATTACGCACCGTGAACAGTGCTgt                         >  2:5934449/1‑92 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         >  1:241595/1‑93 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         <  2:1873970/93‑1 (MQ=255)
                                                                tCCAAGAAACGAACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         >  1:3686095/1‑93 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        <  2:799350/93‑1 (MQ=255)
                                                                  cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt                       >  1:3326270/1‑93 (MQ=255)
                                                                   aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt                      >  2:2870357/1‑93 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc                     <  1:2924893/93‑1 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc                     >  2:6076233/1‑93 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    >  1:4495445/1‑93 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    <  1:932168/93‑1 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    <  1:5745214/93‑1 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGCGCTGATTTCg                    >  2:4771510/1‑93 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 >  2:4994164/1‑93 (MQ=255)
                                                                           caCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              >  2:4762310/2‑93 (MQ=255)
                                                                            aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaac             >  2:3302724/1‑93 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            >  1:3444345/1‑93 (MQ=255)
                                                                              cGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaa           >  2:1384148/1‑93 (MQ=255)
                                                                              cGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGGGCTGATTTCGCCATaacaa           >  1:462906/1‑93 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          >  1:1703568/1‑93 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          <  2:662543/93‑1 (MQ=255)
                                                                                tgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACt         >  2:2140537/1‑93 (MQ=255)
                                                                                 gtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACtt        >  1:839992/1‑93 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  1:4086671/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  2:3035152/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  1:560405/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  2:4647196/93‑1 (MQ=255)
                                                                                   ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  1:1953906/93‑1 (MQ=255)
                                                                                   ttCCCGGAT‑CAAATAACCAGTTTAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  2:5202175/93‑1 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATg     >  1:2745871/1‑93 (MQ=255)
                                                                                     cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGc    <  1:1839728/93‑1 (MQ=255)
                                                                                     cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGc    >  2:3059761/1‑93 (MQ=255)
                                                                                       cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTg  <  1:4022011/93‑1 (MQ=255)
                                                                                            |                                                                                        
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  >  NC_000913/3429436‑3429619
                                                                                           |                                                                                            
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAA                                                                                           >  SRR4302532.868743/1‑94 (MQ=60)
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAA                                                                                           >  SRR4302532.5760539/1‑94 (MQ=60)
  ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                         <  SRR4302532.4817578/94‑1 (MQ=60)
   TGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAA                                                                                        >  SRR4302532.4114250/1‑94 (MQ=60)
   TGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAA                                                                                        >  SRR4302532.5956982/1‑94 (MQ=60)
     TCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACC                                                                                      <  SRR4302532.3244091/94‑1 (MQ=60)
     TCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACC                                                                                      >  SRR4302532.372779/1‑94 (MQ=60)
      CCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCA                                                                                     <  SRR4302532.2175633/94‑1 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                    >  SRR4302532.6230797/1‑94 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                    >  SRR4302532.4691288/1‑94 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                    >  SRR4302532.1816819/1‑94 (MQ=60)
        ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTAAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                   >  SRR4302532.2168228/1‑94 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                <  SRR4302532.581749/94‑1 (MQ=60)
            GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                               <  SRR4302532.4398079/94‑1 (MQ=60)
            GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                               >  SRR4302532.698853/1‑94 (MQ=60)
            GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                               >  SRR4302532.3108725/1‑94 (MQ=60)
            GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                               <  SRR4302532.2183930/94‑1 (MQ=60)
            GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑AGAATAACCAGTTCAG                                                                               >  SRR4302532.698852/1‑94 (MQ=60)
             CTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGC                                                                              <  SRR4302532.558353/94‑1 (MQ=60)
              TGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTGCCGGAT‑CGAATAACCcggtctttc                                                                             >  SRR4302532.5273502/1‑85 (MQ=60)
              TGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCC                                                                             >  SRR4302532.2538619/1‑94 (MQ=60)
                AAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCC                                                                           <  SRR4302532.523227/94‑1 (MQ=60)
                 AGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCG                                                                          <  SRR4302532.4875017/94‑1 (MQ=60)
                    TTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAC                                                                       <  SRR4302532.5340967/94‑1 (MQ=60)
                    TTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAC                                                                       >  SRR4302532.4045850/1‑94 (MQ=60)
                      TCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTT                                                                     <  SRR4302532.4829121/94‑1 (MQ=60)
                       CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTT                                                                    <  SRR4302532.2734523/94‑1 (MQ=60)
                        AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                   >  SRR4302532.901722/1‑94 (MQ=60)
                         GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGG                                                                  <  SRR4302532.168912/94‑1 (MQ=60)
                         GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGG                                                                  <  SRR4302532.1446225/94‑1 (MQ=60)
                          AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                 <  SRR4302532.4125338/94‑1 (MQ=60)
                           GGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGC                                                                >  SRR4302532.5200789/1‑94 (MQ=60)
                           GGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGC                                                                <  SRR4302532.4057631/94‑1 (MQ=60)
                           GGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTAAGCCCCGCCCTTTGGGG                                                                >  SRR4302532.1359348/1‑94 (MQ=60)
                           AGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGC                                                                <  SRR4302532.5283262/94‑1 (MQ=60)
                             ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                              <  SRR4302532.997508/94‑1 (MQ=60)
                             ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                              <  SRR4302532.5938782/94‑1 (MQ=60)
                              CGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGG                                                             >  SRR4302532.5021443/1‑94 (MQ=60)
                              CGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGG                                                             >  SRR4302532.348868/1‑94 (MQ=60)
                               GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                            >  SRR4302532.6054419/1‑94 (MQ=60)
                               GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                            >  SRR4302532.684465/1‑94 (MQ=60)
                                 ACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACT                                                          <  SRR4302532.4922077/94‑1 (MQ=60)
                                   GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                        <  SRR4302532.3285036/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                       <  SRR4302532.5236169/94‑1 (MQ=60)
                                     AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                      >  SRR4302532.3211464/1‑94 (MQ=60)
                                     AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                      <  SRR4302532.5130113/94‑1 (MQ=60)
                                     AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                      >  SRR4302532.1784894/1‑94 (MQ=60)
                                     AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                      <  SRR4302532.980151/94‑1 (MQ=60)
                                      GTCGCACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTA                                                     <  SRR4302532.5737418/94‑1 (MQ=60)
                                      GTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTA                                                     >  SRR4302532.1513450/1‑94 (MQ=60)
                                      GTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTA                                                     <  SRR4302532.421911/94‑1 (MQ=60)
                                      GTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTA                                                     <  SRR4302532.965089/94‑1 (MQ=60)
                                      GTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTA                                                     >  SRR4302532.554448/1‑94 (MQ=60)
                                        CACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATT                                                   >  SRR4302532.301782/1‑94 (MQ=60)
                                         ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                  >  SRR4302532.2469473/1‑94 (MQ=60)
                                          CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                 <  SRR4302532.1482276/94‑1 (MQ=60)
                                          CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                 <  SRR4302532.4369445/94‑1 (MQ=60)
                                          CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                 <  SRR4302532.1588218/94‑1 (MQ=60)
                                              CCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAC                                             <  SRR4302532.3871376/94‑1 (MQ=60)
                                               CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                            >  SRR4302532.3579504/1‑94 (MQ=60)
                                                GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                           >  SRR4302532.5884334/1‑94 (MQ=60)
                                                GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                           <  SRR4302532.4854041/94‑1 (MQ=60)
                                                  ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                         >  SRR4302532.1456005/1‑94 (MQ=60)
                                                  ATACTGTCAGCATCCAAGAAACGGACCGTGTTGCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                         <  SRR4302532.2684794/94‑1 (MQ=60)
                                                     CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                      >  SRR4302532.5967976/1‑94 (MQ=60)
                                                     CTGTGAGCATCCAAGAAACGGACCGTGTTTACCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                      >  SRR4302532.5569921/1‑94 (MQ=60)
                                                      TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                     >  SRR4302532.4373606/1‑94 (MQ=60)
                                                       ggaagcaaCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                    <  SRR4302532.5053796/86‑1 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                    <  SRR4302532.319886/94‑1 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                    <  SRR4302532.4991611/94‑1 (MQ=60)
                                                         GAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTG                                  <  SRR4302532.1411407/94‑1 (MQ=60)
                                                          AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                 >  SRR4302532.4352419/1‑94 (MQ=60)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                <  SRR4302532.1328327/94‑1 (MQ=60)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                <  SRR4302532.385989/94‑1 (MQ=60)
                                                            CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                               >  SRR4302532.385786/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                              >  SRR4302532.3205247/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                              >  SRR4302532.3572324/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                              >  SRR4302532.249941/1‑94 (MQ=60)
                                                             ATCCAAGAAACGAACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                              >  SRR4302532.3809871/1‑94 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                            >  SRR4302532.3437189/1‑94 (MQ=60)
                                                                CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                           >  SRR4302532.2496758/1‑94 (MQ=60)
                                                                 AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                          >  SRR4302532.370666/1‑94 (MQ=60)
                                                                  AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                         <  SRR4302532.3022233/94‑1 (MQ=60)
                                                                  AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                         >  SRR4302532.4648917/1‑94 (MQ=60)
                                                                  AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                         <  SRR4302532.4015693/94‑1 (MQ=60)
                                                                   GAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC                        <  SRR4302532.964106/94‑1 (MQ=60)
                                                                   GAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC                        <  SRR4302532.5945211/94‑1 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                       >  SRR4302532.4921398/1‑94 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                       >  SRR4302532.4325729/1‑94 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                       >  SRR4302532.5354584/1‑94 (MQ=60)
                                                                      ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                     <  SRR4302532.2880518/94‑1 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                   >  SRR4302532.4452768/1‑94 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                  >  SRR4302532.2303788/1‑94 (MQ=60)
                                                                          ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                 >  SRR4302532.3559734/1‑94 (MQ=60)
                                                                           CCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGGGCTGATTTCGCCATAACAA                >  SRR4302532.479021/1‑94 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC               >  SRR4302532.1761023/1‑94 (MQ=60)
                                                                             GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT              <  SRR4302532.4780663/94‑1 (MQ=60)
                                                                              TGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTT             >  SRR4302532.494785/1‑94 (MQ=60)
                                                                              TGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTT             >  SRR4302532.868813/1‑94 (MQ=60)
                                                                              TGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTT             >  SRR4302532.4133777/1‑94 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT           <  SRR4302532.4223851/94‑1 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT           <  SRR4302532.3844964/94‑1 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT           <  SRR4302532.579940/94‑1 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT           <  SRR4302532.5312902/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG          <  SRR4302532.2018821/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG          >  SRR4302532.2838099/1‑94 (MQ=60)
                                                                                 TGCCCGGAT‑CGAATAACCAGTTCAGCCCCGCTCTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG          <  SRR4302532.2018820/94‑1 (MQ=60)
                                                                                  TCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGC         <  SRR4302532.1983890/94‑1 (MQ=60)
                                                                                   CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT        <  SRR4302532.975186/94‑1 (MQ=60)
                                                                                   CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT        <  SRR4302532.1900851/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       >  SRR4302532.5922959/1‑94 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       >  SRR4302532.2134049/1‑94 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       >  SRR4302532.1858086/1‑94 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       <  SRR4302532.4830146/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG       >  SRR4302532.4881271/1‑94 (MQ=60)
                                                                                     CGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGA      <  SRR4302532.4157555/94‑1 (MQ=60)
                                                                                      GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA     <  SRR4302532.4339194/94‑1 (MQ=60)
                                                                                       GAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCATGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT    >  SRR4302532.4110336/1‑94 (MQ=60)
                                                                                       GAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT    >  SRR4302532.2388296/1‑94 (MQ=60)
                                                                                       GAT‑CGAATAACCAGTTCAGCCCCGCACTTAGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT    <  SRR4302532.3799/94‑1 (MQ=60)
                                                                                         atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT   >  SRR4302532.887081/3‑94 (MQ=60)
                                                                                         atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT   >  SRR4302532.1774084/3‑94 (MQ=60)
                                                                                         atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT   >  SRR4302532.3250802/3‑94 (MQ=60)
                                                                                          TCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  <  SRR4302532.2279523/94‑1 (MQ=60)
                                                                                           |                                                                                            
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA  >  NC_000913/3429436‑3429619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑