Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,429,527 Δ1 bp coding (40/510 nt) yrdD ← putative DNA topoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,429,5260C.100.0% 1064.1 / NA 225coding (41/510 nt)yrdDputative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (119/106);  total (119/106)

Only 100 of 241 total aligned reads displayed.

CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614
                                                                                            |                                                                                        
 gATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg                                                                                         >  2:856753/1‑92 (MQ=255)
 gATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg                                                                                         >  2:2307473/1‑92 (MQ=255)
  atatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga                                                                                        >  1:5628805/1‑91 (MQ=255)
    atGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaat                                                                                      >  2:1467513/1‑89 (MQ=255)
        ccATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAcc                                                                                 >  2:5041744/1‑93 (MQ=255)
         cATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa                                                                                >  2:641611/1‑93 (MQ=255)
          aTCCGCTGAAGATTTCAGAGGGCGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg                                                                               >  2:6596883/1‑93 (MQ=255)
          aTCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg                                                                               <  1:5243342/93‑1 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              >  2:5150093/1‑93 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              <  1:4180166/93‑1 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              >  2:2946687/1‑93 (MQ=255)
            ccGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtt                                                                             >  1:993915/1‑93 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            >  2:3896015/1‑93 (MQ=255)
              gCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa                                                                           <  2:4296013/93‑1 (MQ=255)
                tGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGNAANGGNCCGTGTTTCACGGAT‑CGAATAACCAGTTCAGc                                                                         >  1:5007033/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        <  1:6112547/93‑1 (MQ=255)
                  aaGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGccc                                                                       >  2:5279950/1‑93 (MQ=255)
                   aGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc                                                                      >  2:185463/1‑93 (MQ=255)
                   aGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcacc                                                                      >  2:5344014/1‑93 (MQ=255)
                    gATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg                                                                     >  2:4958345/1‑93 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  <  2:4367636/93‑1 (MQ=255)
                          agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGGATAACCAGATCAGTCCCGCAAttt                                                               >  1:5491258/1‑93 (MQ=255)
                          agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt                                                               <  1:5437632/93‑1 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              >  2:3506319/1‑93 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              <  2:3696742/93‑1 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              >  1:4669883/1‑93 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              <  1:1076204/93‑1 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑GGAATAACCAGTTCACCCCCCCACttttg                                                             >  1:6286480/1‑91 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             <  1:598690/93‑1 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             <  1:1331411/93‑1 (MQ=255)
                             ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            <  2:5554091/93‑1 (MQ=255)
                             ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            >  1:6350233/1‑93 (MQ=255)
                             ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            >  2:2041363/1‑93 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       >  1:4389817/1‑93 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       >  2:1426379/1‑93 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      <  1:1566519/93‑1 (MQ=255)
                                    cgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACt                                                     <  2:3555965/93‑1 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   >  1:795509/1‑93 (MQ=255)
                                       aGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat                                                  <  1:290804/93‑1 (MQ=255)
                                         tCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatta                                                >  2:1786279/1‑93 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              <  1:1135426/93‑1 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              <  1:2880349/93‑1 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              >  1:3518377/1‑93 (MQ=255)
                                             acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc                                            >  2:2446967/1‑93 (MQ=255)
                                             acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc                                            >  2:1345946/1‑93 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc                                        <  2:4901070/93‑1 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc                                        >  2:3231396/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  1:4910234/93‑1 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  1:2282495/93‑1 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       >  1:5145593/1‑93 (MQ=255)
                                                   gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg                                      <  2:5995033/93‑1 (MQ=255)
                                                   gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg                                      <  1:3504835/93‑1 (MQ=255)
                                                    aTACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGt                                     >  2:5890093/1‑93 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    <  1:481139/93‑1 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    >  2:6309908/1‑93 (MQ=255)
                                                      aCTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGa                                   >  1:165880/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:1029215/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:2928546/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  2:257578/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:1005482/93‑1 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                >  1:5876810/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  2:3314569/93‑1 (MQ=255)
                                                           gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt                              <  1:4517893/93‑1 (MQ=255)
                                                            aGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTg                             <  1:2467205/93‑1 (MQ=255)
                                                            aGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTg                             <  1:2828331/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  1:3654490/1‑93 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  1:2618616/1‑93 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            <  1:4266078/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  2:3100350/1‑93 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt                           <  2:5401668/93‑1 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt                           <  1:2522016/93‑1 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt                           <  1:1948431/93‑1 (MQ=255)
                                                               aTCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg                          >  1:4341507/1‑93 (MQ=255)
                                                               aTCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTg                          >  1:6417719/1‑93 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         <  1:3941853/93‑1 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        >  1:409258/1‑93 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        <  2:2114197/93‑1 (MQ=255)
                                                                  cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt                       <  1:4952400/93‑1 (MQ=255)
                                                                   aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt                      >  1:3243906/1‑93 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc                     <  2:341578/93‑1 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTc                     <  1:2114908/93‑1 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    >  2:2742367/1‑93 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    >  1:3897582/1‑93 (MQ=255)
                                                                      aaaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGc                   >  2:5931818/1‑93 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 <  2:616516/93‑1 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 <  1:5687912/93‑1 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 <  1:3697100/93‑1 (MQ=255)
                                                                         cGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAt                >  2:3853623/1‑93 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTACGCCATaa              <  1:6457915/93‑1 (MQ=255)
                                                                            gccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaac             <  1:6081732/92‑1 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            >  2:5772779/1‑93 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            <  2:4647097/93‑1 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            <  2:4380136/93‑1 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGCTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            >  2:965994/1‑93 (MQ=255)
                                                                                 gtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACtt        <  2:3761094/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       >  1:5201686/1‑93 (MQ=255)
                                                                                   ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  2:4801820/93‑1 (MQ=255)
                                                                                   ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  2:693938/93‑1 (MQ=255)
                                                                                      ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCt   >  1:5385511/1‑93 (MQ=255)
                                                                                       cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCCTTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTg  <  1:6555029/93‑1 (MQ=255)
                                                                                            |                                                                                        
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  NC_000913/3429434‑3429620
                                                                                             |                                                                                             
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACGCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               <  SRR4302547.4995322/94‑1 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               <  SRR4302547.6092522/94‑1 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               <  SRR4302547.630436/94‑1 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               >  SRR4302547.2230548/1‑94 (MQ=60)
  ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAA                                                                                            >  SRR4302547.6542277/1‑94 (MQ=60)
    ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                          <  SRR4302547.4646349/94‑1 (MQ=60)
    ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                          >  SRR4302547.1408217/1‑94 (MQ=60)
         CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                     >  SRR4302547.5376798/1‑94 (MQ=60)
         CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                     <  SRR4302547.3710577/94‑1 (MQ=60)
          ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                    <  SRR4302547.5351261/94‑1 (MQ=60)
          ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                    <  SRR4302547.4423721/94‑1 (MQ=60)
          ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                    >  SRR4302547.3820219/1‑94 (MQ=60)
           TCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTT                                                                                   >  SRR4302547.1013279/1‑94 (MQ=60)
           TCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTT                                                                                   <  SRR4302547.4265355/94‑1 (MQ=60)
            CCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTC                                                                                  <  SRR4302547.129787/94‑1 (MQ=60)
             CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                 <  SRR4302547.6649809/94‑1 (MQ=60)
              GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                                >  SRR4302547.4016262/1‑94 (MQ=60)
              GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                                >  SRR4302547.70710/1‑94 (MQ=60)
               CTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGNAANGGNCCGTGTTTCACGGAT‑CGAATAACCAGTTCAGC                                                                               >  SRR4302547.5110560/1‑94 (MQ=60)
                TGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCC                                                                              >  SRR4302547.1066741/1‑94 (MQ=60)
                 GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCC                                                                             <  SRR4302547.6239347/94‑1 (MQ=60)
                  AAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCC                                                                            <  SRR4302547.5302337/94‑1 (MQ=60)
                  AAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCC                                                                            >  SRR4302547.4292731/1‑94 (MQ=60)
                  AAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCC                                                                            <  SRR4302547.2528320/94‑1 (MQ=60)
                   AGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCG                                                                           >  SRR4302547.5602752/1‑94 (MQ=60)
                   AGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCG                                                                           >  SRR4302547.5049969/1‑94 (MQ=60)
                    GATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGC                                                                          <  SRR4302547.4099949/94‑1 (MQ=60)
                      TTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAC                                                                        >  SRR4302547.4445977/1‑94 (MQ=60)
                         CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGGATAACCAGATCAGTCCCGCAATTT                                                                     >  SRR4302547.5604645/1‑94 (MQ=60)
                          AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    >  SRR4302547.4766589/1‑94 (MQ=60)
                          AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    >  SRR4302547.3410840/1‑94 (MQ=60)
                          AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    <  SRR4302547.5550008/94‑1 (MQ=60)
                           GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑GGAATAACCAGTTCACCCCCCCACTTTTG                                                                   >  SRR4302547.6417096/1‑94 (MQ=60)
                           GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGG                                                                   <  SRR4302547.1097681/94‑1 (MQ=60)
                            AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                  <  SRR4302547.609731/94‑1 (MQ=60)
                            AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                  >  SRR4302547.6482560/1‑94 (MQ=60)
                            AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                  <  SRR4302547.1358173/94‑1 (MQ=60)
                             GGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGC                                                                 <  SRR4302547.4617100/94‑1 (MQ=60)
                                CGTACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGG                                                              <  SRR4302547.5529076/94‑1 (MQ=60)
                                 GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                             >  SRR4302547.4480148/1‑94 (MQ=60)
                                  GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                            >  SRR4302547.4958622/1‑94 (MQ=60)
                                   ACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACT                                                           <  SRR4302547.1597716/94‑1 (MQ=60)
                                     GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         >  SRR4302547.3585138/1‑94 (MQ=60)
                                     GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         >  SRR4302547.810567/1‑94 (MQ=60)
                                      TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302547.5940072/94‑1 (MQ=60)
                                       AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                       <  SRR4302547.296202/94‑1 (MQ=60)
                                          CACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATT                                                    >  SRR4302547.3590195/1‑94 (MQ=60)
                                           ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   >  SRR4302547.4217055/1‑94 (MQ=60)
                                           ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   <  SRR4302547.1158356/94‑1 (MQ=60)
                                           ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   <  SRR4302547.2937836/94‑1 (MQ=60)
                                            CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                  >  SRR4302547.5785936/1‑94 (MQ=60)
                                                 CGGATACTGTGCGCATCCGAGAGACGGACCGTGTTTCCCGGAT‑CGATTAACCGATTCCGCCCCGCTCTTTGAGCAGGTCTCATTATTACGCtca                                             <  SRR4302547.6792291/94‑4 (MQ=23)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             <  SRR4302547.1673972/94‑1 (MQ=60)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             <  SRR4302547.5202585/94‑1 (MQ=60)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             <  SRR4302547.5090531/94‑1 (MQ=60)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             >  SRR4302547.2964884/1‑94 (MQ=60)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             >  SRR4302547.5251577/1‑94 (MQ=60)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             >  SRR4302547.573339/1‑94 (MQ=60)
                                                  GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            >  SRR4302547.6193422/1‑94 (MQ=60)
                                                  GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            <  SRR4302547.2328698/94‑1 (MQ=60)
                                                  GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            >  SRR4302547.2152129/1‑94 (MQ=60)
                                                  GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCA                                            <  SRR4302547.5011915/94‑1 (MQ=60)
                                                   GATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGT                                           <  SRR4302547.3576359/94‑1 (MQ=60)
                                                    ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                          <  SRR4302547.5249470/94‑1 (MQ=60)
                                                    ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                          >  SRR4302547.6420909/1‑94 (MQ=60)
                                                    ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                          >  SRR4302547.3643349/1‑94 (MQ=60)
                                                     TACTGTGAGGATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGGTCAGATCCGCACTATGGGCAGCACTGATGATTACGCACgggga                                         >  SRR4302547.1153172/1‑89 (MQ=39)
                                                     TACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGA                                         <  SRR4302547.489969/94‑1 (MQ=60)
                                                     TACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGA                                         >  SRR4302547.4537177/1‑94 (MQ=60)
                                                     TACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGA                                         >  SRR4302547.168928/1‑94 (MQ=60)
                                                     TACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGA                                         >  SRR4302547.631501/1‑94 (MQ=60)
                                                      ACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAA                                        <  SRR4302547.2806426/94‑1 (MQ=60)
                                                      ACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAA                                        >  SRR4302547.2758158/1‑94 (MQ=60)
                                                      ACTGTGAGCATCCAAGAAACGCACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAA                                        <  SRR4302547.4423657/94‑1 (MQ=60)
                                                       CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                       >  SRR4302547.4235734/1‑94 (MQ=60)
                                                       CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                       >  SRR4302547.2986934/1‑94 (MQ=60)
                                                       CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                       >  SRR4302547.1049433/1‑94 (MQ=60)
                                                        TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      >  SRR4302547.2981080/1‑94 (MQ=60)
                                                        TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      >  SRR4302547.5998479/1‑94 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302547.1025102/94‑1 (MQ=60)
                                                           GAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTG                                   <  SRR4302547.488099/94‑1 (MQ=60)
                                                           GAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTG                                   <  SRR4302547.4611226/94‑1 (MQ=60)
                                                            AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  <  SRR4302547.2884888/94‑1 (MQ=60)
                                                            AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  >  SRR4302547.2671378/1‑94 (MQ=60)
                                                            AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  <  SRR4302547.2517077/94‑1 (MQ=60)
                                                            AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  >  SRR4302547.3729039/1‑94 (MQ=60)
                                                             GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 <  SRR4302547.4353505/94‑1 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                >  SRR4302547.4430778/1‑94 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                <  SRR4302547.60794/94‑1 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                <  SRR4302547.2572984/94‑1 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                <  SRR4302547.1987057/94‑1 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                >  SRR4302547.6551528/1‑94 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                <  SRR4302547.3824667/94‑1 (MQ=60)
                                                               ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               <  SRR4302547.2387010/94‑1 (MQ=60)
                                                               ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CAAATAACCAGTTCAGCCCGGCACTTTGGGCAGGACTCATTATTACGCTCCGTGAACAGTGCTGA                               <  SRR4302547.713210/94‑1 (MQ=60)
                                                                TCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAT                              <  SRR4302547.4022011/94‑1 (MQ=60)
                                                                TCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAT                              >  SRR4302547.416848/1‑94 (MQ=60)
                                                                 CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             >  SRR4302547.3023466/1‑94 (MQ=60)
                                                                  CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            <  SRR4302547.5054931/94‑1 (MQ=60)
                                                                  CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            >  SRR4302547.3308695/1‑94 (MQ=60)
                                                                   AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                           >  SRR4302547.831461/1‑94 (MQ=60)
                                                                   AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                           <  SRR4302547.294001/94‑1 (MQ=60)
                                                                    AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                          >  SRR4302547.3976833/1‑94 (MQ=60)
                                                                    AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                          <  SRR4302547.2157050/94‑1 (MQ=60)
                                                                     GAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC                         >  SRR4302547.3749810/1‑94 (MQ=60)
                                                                      AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        >  SRR4302547.5347313/1‑94 (MQ=60)
                                                                      AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        >  SRR4302547.3537662/1‑94 (MQ=60)
                                                                        ACGGACCGTGTTTCCCGGAT‑GGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                      >  SRR4302547.6549139/1‑94 (MQ=60)
                                                                        ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                      <  SRR4302547.2410831/94‑1 (MQ=60)
                                                                        ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                      <  SRR4302547.3772560/94‑1 (MQ=60)
                                                                        ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                      <  SRR4302547.5805359/94‑1 (MQ=60)
                                                                          GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    >  SRR4302547.5309440/1‑94 (MQ=60)
                                                                          GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    >  SRR4302547.1105119/1‑94 (MQ=60)
                                                                           GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTACGCCATAAC                   <  SRR4302547.6592415/94‑1 (MQ=60)
                                                                            GCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                  <  SRR4302547.6207909/94‑1 (MQ=60)
                                                                            ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                  >  SRR4302547.6558996/1‑94 (MQ=60)
                                                                             CCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAA                 >  SRR4302547.1691956/1‑94 (MQ=60)
                                                                               GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               <  SRR4302547.2019039/94‑1 (MQ=60)
                                                                               GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               <  SRR4302547.1136548/94‑1 (MQ=60)
                                                                                 GTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTA             >  SRR4302547.5308721/1‑94 (MQ=60)
                                                                                 GTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTA             >  SRR4302547.5596454/1‑94 (MQ=60)
                                                                                  TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT            <  SRR4302547.289970/94‑1 (MQ=60)
                                                                                  TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT            <  SRR4302547.2603888/94‑1 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTTCTGATTTCGCCATAACAACTTATGCT         <  SRR4302547.5615302/94‑1 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT         >  SRR4302547.5496716/1‑94 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT         >  SRR4302547.619559/1‑94 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT         >  SRR4302547.1048928/1‑94 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCGGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302547.1367229/94‑1 (MQ=60)
                                                                                       CGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCCTTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGA       <  SRR4302547.6691522/94‑1 (MQ=60)
                                                                                        GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTTAA      <  SRR4302547.6685691/94‑1 (MQ=60)
                                                                                        GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA      <  SRR4302547.2966432/94‑1 (MQ=60)
                                                                                        GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA      >  SRR4302547.1800056/1‑94 (MQ=60)
                                                                                        GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA      <  SRR4302547.3439826/94‑1 (MQ=60)
                                                                                         GAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT     >  SRR4302547.3366015/1‑94 (MQ=60)
                                                                                         GAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT     <  SRR4302547.3729256/94‑1 (MQ=60)
                                                                                           atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT    <  SRR4302547.2712028/92‑1 (MQ=60)
                                                                                           atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT    >  SRR4302547.5042477/3‑94 (MQ=60)
                                                                                            TCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA   <  SRR4302547.3069016/94‑1 (MQ=60)
                                                                                             CGAATAACCAGTTCAGCCCCGCACTTTGGGCAAGCCTCATTATTAAGCACCGTGAACAGTACTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302547.3075619/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  NC_000913/3429434‑3429620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑