Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,252 C→T P744S (CCG→TCG)  b3407 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,2520CT96.3% 769.7 / 9.0 216P744S (CCG→TCG) b3407hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base T (120/88);  total (125/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

Only 100 of 225 total aligned reads displayed.

AACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGT  >  NC_000913/3537160‑3537341
                                                                                            |                                                                                         
aaCGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGcc                                                                                           <  2:4485226/93‑1 (MQ=255)
  cGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCg                                                                                         <  2:2009808/93‑1 (MQ=255)
   gTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCgg                                                                                        >  2:1761165/1‑93 (MQ=255)
       cGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGc                                                                                    >  2:1351769/1‑93 (MQ=255)
        gCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAACACAACCGCTCGGCAGcc                                                                                   >  1:3388006/1‑93 (MQ=255)
        gCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGcc                                                                                   >  2:1154758/1‑93 (MQ=255)
         cccTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCa                                                                                  <  2:2538225/93‑1 (MQ=255)
         cccTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCa                                                                                  >  2:3046492/1‑93 (MQ=255)
          ccTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCaa                                                                                 >  2:5948029/1‑93 (MQ=255)
           cTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGTCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCaaa                                                                                >  1:3963215/1‑93 (MQ=255)
            tGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAAc                                                                               >  2:3136463/1‑93 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             >  1:4944243/1‑93 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             <  1:5138122/93‑1 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             >  2:4994579/1‑93 (MQ=255)
              aCTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCa                                                                             >  2:5862392/1‑93 (MQ=255)
               cTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAc                                                                            <  1:2140716/93‑1 (MQ=255)
                taggcgcCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAcg                                                                           <  2:1345002/90‑1 (MQ=255)
                tATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACTCCCGCAAAACAACCGCTCGGCAGCCAAACCAcc                                                                           >  2:3763661/1‑92 (MQ=255)
                tATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAcg                                                                           <  1:4212742/93‑1 (MQ=255)
                  tGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAcgcg                                                                         >  1:1180570/1‑93 (MQ=255)
                    cgcCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGt                                                                       <  1:4860366/93‑1 (MQ=255)
                    cgcCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGt                                                                       <  2:1329810/93‑1 (MQ=255)
                      ccTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCg                                                                     >  1:5986880/1‑93 (MQ=255)
                      ccTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCg                                                                     >  1:2560542/1‑93 (MQ=255)
                        tGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTg                                                                   <  1:5594953/93‑1 (MQ=255)
                        tGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTg                                                                   >  2:1425985/1‑93 (MQ=255)
                        tGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTg                                                                   >  2:3031500/1‑93 (MQ=255)
                         ggATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGa                                                                  <  2:4602389/93‑1 (MQ=255)
                             gAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAgcg                                                              >  1:4788963/1‑93 (MQ=255)
                              agcagcCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAgcgc                                                             >  2:5141628/1‑93 (MQ=255)
                               gcagcCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCa                                                            <  2:2991383/93‑1 (MQ=255)
                                  gcCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGcc                                                         >  1:2937948/1‑93 (MQ=255)
                                    cTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTg                                                       >  2:4777345/1‑93 (MQ=255)
                                    cTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTg                                                       >  1:4440787/1‑93 (MQ=255)
                                     tGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGc                                                      >  1:4646088/1‑93 (MQ=255)
                                         gAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGt                                                  >  2:4123103/1‑93 (MQ=255)
                                         gAAACCAACGCTCGTCGCGGCGGCGGTAATGAACACCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCgga                                                  >  2:3584488/1‑92 (MQ=255)
                                            aCCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAAt                                               >  1:1811419/1‑93 (MQ=255)
                                            aCCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAAt                                               >  2:2914382/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              >  2:5240636/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              >  1:1568028/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              >  2:2158088/1‑93 (MQ=255)
                                             ccAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATa                                              <  1:1149413/93‑1 (MQ=255)
                                              cAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAg                                             <  2:1858369/93‑1 (MQ=255)
                                               aaCGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAgc                                            <  1:2800099/93‑1 (MQ=255)
                                                 cGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAgcgc                                          <  2:5057324/93‑1 (MQ=255)
                                                  gCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGcgcg                                         >  1:1673614/1‑93 (MQ=255)
                                                  gCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGcgcg                                         >  2:169777/1‑93 (MQ=255)
                                                   cTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCga                                        <  2:5434169/93‑1 (MQ=255)
                                                    tcgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgat                                       >  1:4140192/1‑93 (MQ=255)
                                                    tcgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgat                                       >  2:6029659/1‑93 (MQ=255)
                                                     cgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgatg                                      <  2:2073202/93‑1 (MQ=255)
                                                     cgtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgatg                                      >  1:219924/1‑93 (MQ=255)
                                                      gtcgCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCgatga                                     <  1:3782285/93‑1 (MQ=255)
                                                        cgcgGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGatgatg                                   <  2:3949186/93‑1 (MQ=255)
                                                         gcggcggcgGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgg                                  >  1:3318382/1‑93 (MQ=255)
                                                         gcggcggcgGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgg                                  >  1:540611/1‑93 (MQ=255)
                                                          cggcggcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgga                                 <  1:1409028/93‑1 (MQ=255)
                                                          cggcggcggTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatgga                                 <  2:5710484/93‑1 (MQ=255)
                                                           ggcggcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATgatggat                                <  1:5783559/93‑1 (MQ=255)
                                                              ggcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATgcg                             >  1:1023051/1‑93 (MQ=255)
                                                               gcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATgcgc                            <  2:3307906/93‑1 (MQ=255)
                                                               gcggTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATgcgc                            >  1:4379348/1‑93 (MQ=255)
                                                                  gTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTgg                         <  2:4645468/93‑1 (MQ=255)
                                                                   tAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggc                        <  2:1054717/93‑1 (MQ=255)
                                                                   tAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggc                        >  2:617134/1‑93 (MQ=255)
                                                                   tAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggc                        >  1:4237184/1‑93 (MQ=255)
                                                                    aaTGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcg                       >  2:859263/1‑93 (MQ=255)
                                                                     aTGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcgg                      <  2:3641797/93‑1 (MQ=255)
                                                                      tGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggg                     >  2:2269347/1‑92 (MQ=255)
                                                                      tGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggc                     >  1:3049461/1‑93 (MQ=255)
                                                                      tGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggc                     >  2:4884547/1‑93 (MQ=255)
                                                                       gAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                    >  1:4431794/1‑93 (MQ=255)
                                                                       gAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                    >  1:369657/1‑93 (MQ=255)
                                                                       gAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcg                    >  1:5314875/1‑93 (MQ=255)
                                                                        aaCGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcgg                   >  2:2377539/1‑93 (MQ=255)
                                                                        aaCGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTggcggcgg                   >  1:3352019/1‑93 (MQ=255)
                                                                          cGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCa                 >  1:4518241/1‑93 (MQ=255)
                                                                            cccGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAAt               <  1:3850002/93‑1 (MQ=255)
                                                                             ccGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATg              >  2:886996/1‑93 (MQ=255)
                                                                             ccGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATg              >  1:1908006/1‑93 (MQ=255)
                                                                              cGCAACACAACCGCTCATCAACCAAACCGCGCGGTCGTGAAGCACAGCCTGCCGGTAATAGCGCCATGATGGATGCGCTGGCAGCGGCAATgg             <  2:5875180/93‑1 (MQ=255)
                                                                              cGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATgg             <  2:3548211/93‑1 (MQ=255)
                                                                              cGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATgg             >  1:2457848/1‑93 (MQ=255)
                                                                              cGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATgg             >  1:3540772/1‑93 (MQ=255)
                                                                                 aaaaGAACCGCTCGGCAGCAAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCa          <  1:3831826/93‑1 (MQ=255)
                                                                                 aaaaCAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCa          >  2:1144106/1‑93 (MQ=255)
                                                                                 aaaaCAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCa          >  1:722331/1‑93 (MQ=255)
                                                                                  aaaCAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaa         >  1:1285302/1‑93 (MQ=255)
                                                                                   aacaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaa        >  1:2952665/1‑93 (MQ=255)
                                                                                   aacaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaa        >  1:2892074/1‑93 (MQ=255)
                                                                                   aacaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaa        >  2:4570264/1‑93 (MQ=255)
                                                                                    acaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaa       <  2:2036513/93‑1 (MQ=255)
                                                                                    acaacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaa       >  2:706657/1‑93 (MQ=255)
                                                                                     caacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaa      <  1:1993195/93‑1 (MQ=255)
                                                                                      ccccGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaaa     <  2:1603920/91‑1 (MQ=255)
                                                                                      aacCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCaaaaaa     <  1:4830686/93‑1 (MQ=255)
                                                                                       acCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAAc    >  1:5845155/1‑93 (MQ=255)
                                                                                       acCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAAc    >  2:2798749/1‑93 (MQ=255)
                                                                                         cGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGt  >  1:5679053/1‑93 (MQ=255)
                                                                                            |                                                                                         
AACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGT  >  NC_000913/3537160‑3537341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAA  >  NC_000913/3537159‑3537345
                                                                                             |                                                                                             
AAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCT                                                                                               <  SRR4302532.5402225/94‑1 (MQ=60)
 AACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTC                                                                                              <  SRR4302532.1520761/94‑1 (MQ=60)
   CGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGG                                                                                            <  SRR4302532.4412284/94‑1 (MQ=60)
     TATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCA                                                                                          >  SRR4302532.5984811/1‑94 (MQ=60)
      ATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAG                                                                                         <  SRR4302532.5025742/94‑1 (MQ=60)
        CGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAACACAACCGCTCGGCAGCC                                                                                       >  SRR4302532.3501314/1‑94 (MQ=60)
           CCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGTCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAA                                                                                    >  SRR4302532.4096744/1‑94 (MQ=60)
           CCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAA                                                                                    >  SRR4302532.5280277/1‑94 (MQ=60)
            CTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAAC                                                                                   >  SRR4302532.5412648/1‑94 (MQ=60)
             TGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACC                                                                                  >  SRR4302532.4595546/1‑94 (MQ=60)
             TGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACC                                                                                  <  SRR4302532.5856433/94‑1 (MQ=60)
             TGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACC                                                                                  >  SRR4302532.4269336/1‑94 (MQ=60)
              GACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCA                                                                                 >  SRR4302532.5113942/1‑94 (MQ=60)
              GACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCA                                                                                 >  SRR4302532.4913255/1‑94 (MQ=60)
              GACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCA                                                                                 >  SRR4302532.1932467/1‑94 (MQ=60)
               ACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAC                                                                                <  SRR4302532.2646240/94‑1 (MQ=60)
               ACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAC                                                                                <  SRR4302532.2459304/94‑1 (MQ=60)
               ACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCAC                                                                                <  SRR4302532.5314881/94‑1 (MQ=60)
                CTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACG                                                                               <  SRR4302532.2212211/94‑1 (MQ=60)
                CTATGAGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACG                                                                               <  SRR4302532.1102177/94‑1 (MQ=60)
                 TATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGC                                                                              <  SRR4302532.4354147/94‑1 (MQ=60)
                 TATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGGCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGC                                                                              >  SRR4302532.3867230/1‑94 (MQ=60)
                  ATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCG                                                                             >  SRR4302532.1220008/1‑94 (MQ=60)
                   TGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGG                                                                            <  SRR4302532.3038725/94‑1 (MQ=60)
                     CGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTC                                                                          <  SRR4302532.3852522/94‑1 (MQ=60)
                     CGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTC                                                                          <  SRR4302532.5027567/94‑1 (MQ=60)
                      GCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCG                                                                         >  SRR4302532.6193736/1‑94 (MQ=60)
                      GCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCG                                                                         >  SRR4302532.2645965/1‑94 (MQ=60)
                        CTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTG                                                                       >  SRR4302532.1496708/1‑94 (MQ=60)
                         TGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGA                                                                      <  SRR4302532.5789173/94‑1 (MQ=60)
                          GGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAA                                                                     <  SRR4302532.4360149/94‑1 (MQ=60)
                            ATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGC                                                                   >  SRR4302532.2563720/1‑94 (MQ=60)
                             TGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCG                                                                  >  SRR4302532.4953896/1‑94 (MQ=60)
                                  AGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCC                                                             >  SRR4302532.3657945/1‑94 (MQ=60)
                                  AGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCC                                                             >  SRR4302532.3459975/1‑94 (MQ=60)
                                  AGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCC                                                             >  SRR4302532.3035546/1‑94 (MQ=60)
                                    CCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTG                                                           <  SRR4302532.5774401/94‑1 (MQ=60)
                                    CCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTG                                                           >  SRR4302532.4592105/1‑94 (MQ=60)
                                    CCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTG                                                           <  SRR4302532.5046287/94‑1 (MQ=60)
                                     CTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGC                                                          >  SRR4302532.4804492/1‑94 (MQ=60)
                                       GGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCG                                                        <  SRR4302532.5137149/94‑1 (MQ=60)
                                         CGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGT                                                      >  SRR4302532.3496793/1‑94 (MQ=60)
                                          CAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTA                                                     <  SRR4302532.1436893/94‑1 (MQ=60)
                                            AACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAAT                                                   >  SRR4302532.1650084/1‑94 (MQ=60)
                                            AACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAAT                                                   >  SRR4302532.1871829/1‑94 (MQ=60)
                                             ACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATA                                                  <  SRR4302532.1236130/94‑1 (MQ=60)
                                             ACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATA                                                  >  SRR4302532.1803729/1‑94 (MQ=60)
                                             ACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATA                                                  >  SRR4302532.1621090/1‑94 (MQ=60)
                                              CCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAG                                                 >  SRR4302532.2000335/1‑94 (MQ=60)
                                              CCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAG                                                 <  SRR4302532.1187782/94‑1 (MQ=60)
                                               CAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGC                                                <  SRR4302532.1201263/94‑1 (MQ=60)
                                               CAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGC                                                >  SRR4302532.5946289/1‑94 (MQ=60)
                                                AACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCG                                               <  SRR4302532.2893926/94‑1 (MQ=60)
                                                 ACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGC                                              <  SRR4302532.846648/94‑1 (MQ=60)
                                                 ACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGC                                              >  SRR4302532.3025378/1‑94 (MQ=60)
                                                  CGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCG                                             >  SRR4302532.1730213/1‑94 (MQ=60)
                                                   GCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGA                                            <  SRR4302532.4303211/94‑1 (MQ=60)
                                                    CTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGAT                                           >  SRR4302532.3904365/1‑94 (MQ=60)
                                                    CTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGAT                                           >  SRR4302532.4279082/1‑94 (MQ=60)
                                                    CTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGAG                                           >  SRR4302532.4888681/1‑94 (MQ=60)
                                                     TCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATG                                          >  SRR4302532.227427/1‑94 (MQ=60)
                                                       GTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGAT                                        <  SRR4302532.2908646/94‑1 (MQ=60)
                                                       GTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGAT                                        <  SRR4302532.4573882/94‑1 (MQ=60)
                                                       GTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGAT                                        <  SRR4302532.3909778/94‑1 (MQ=60)
                                                       GTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGAT                                        <  SRR4302532.6249674/94‑1 (MQ=60)
                                                        TCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATG                                       <  SRR4302532.6192916/94‑1 (MQ=60)
                                                         CGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGG                                      >  SRR4302532.3429028/1‑94 (MQ=60)
                                                         CGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGG                                      >  SRR4302532.559446/1‑94 (MQ=60)
                                                           CGGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGAT                                    <  SRR4302532.1456261/94‑1 (MQ=60)
                                                            GGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATG                                   <  SRR4302532.5984706/94‑1 (MQ=60)
                                                            GGCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATG                                   >  SRR4302532.2066273/1‑94 (MQ=60)
                                                             GCGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGC                                  <  SRR4302532.4553781/94‑1 (MQ=60)
                                                              CGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCG                                 <  SRR4302532.2264509/94‑1 (MQ=60)
                                                              CGGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCG                                 >  SRR4302532.1057698/1‑94 (MQ=60)
                                                               GGCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGC                                >  SRR4302532.4528076/1‑94 (MQ=60)
                                                                GCGGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCT                               <  SRR4302532.2450889/94‑1 (MQ=60)
                                                                  GGTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGG                             <  SRR4302532.5856419/94‑1 (MQ=60)
                                                                   GTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGC                            >  SRR4302532.4379488/1‑94 (MQ=60)
                                                                   GTAATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGC                            <  SRR4302532.4201728/94‑1 (MQ=60)
                                                                      ATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGC                         >  SRR4302532.3150125/1‑94 (MQ=60)
                                                                      ATGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGC                         <  SRR4302532.4699014/94‑1 (MQ=60)
                                                                       TGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCG                        >  SRR4302532.4582794/1‑94 (MQ=60)
                                                                       TGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCG                        >  SRR4302532.5498721/1‑94 (MQ=60)
                                                                       TGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCG                        <  SRR4302532.3443802/94‑1 (MQ=60)
                                                                       TGAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCG                        >  SRR4302532.382501/1‑94 (MQ=60)
                                                                        GAACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGG                       >  SRR4302532.3463927/1‑94 (MQ=60)
                                                                          ACGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCA                     >  SRR4302532.4672484/1‑94 (MQ=60)
                                                                           CGCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAA                    >  SRR4302532.1886888/1‑94 (MQ=60)
                                                                            GCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAAT                   <  SRR4302532.2997883/94‑1 (MQ=60)
                                                                            GCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAAT                   <  SRR4302532.3690751/94‑1 (MQ=60)
                                                                            GCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAAT                   >  SRR4302532.3217418/1‑94 (MQ=60)
                                                                            GCCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAAT                   >  SRR4302532.3074935/1‑94 (MQ=60)
                                                                             CCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATG                  >  SRR4302532.4020242/1‑94 (MQ=60)
                                                                             CCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATG                  <  SRR4302532.3980001/94‑1 (MQ=60)
                                                                             CCCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATG                  >  SRR4302532.1971325/1‑94 (MQ=60)
                                                                             CACGCAAAACAACCGCGCGGCAGCCAACCCACGCGGTCGTAAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATG                  <  SRR4302532.3964038/94‑1 (MQ=60)
                                                                              CCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGG                 >  SRR4302532.4587197/1‑94 (MQ=60)
                                                                              CCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGG                 >  SRR4302532.5545104/1‑94 (MQ=60)
                                                                              CCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGG                 >  SRR4302532.3659234/1‑94 (MQ=60)
                                                                              CCGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGG                 >  SRR4302532.2539606/1‑94 (MQ=60)
                                                                               CGCAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGG                >  SRR4302532.1348808/1‑94 (MQ=60)
                                                                                 CAAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCA              >  SRR4302532.746971/1‑94 (MQ=60)
                                                                                  AAAAGAACCGCTCGGCAGCAAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAA             <  SRR4302532.3961193/94‑1 (MQ=60)
                                                                                  AAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAA             >  SRR4302532.1328289/1‑94 (MQ=60)
                                                                                  AAAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAA             <  SRR4302532.3961192/94‑1 (MQ=60)
                                                                                   AAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAA            >  SRR4302532.3050544/1‑94 (MQ=60)
                                                                                   AAACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAA            >  SRR4302532.2988680/1‑94 (MQ=60)
                                                                                     ACAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAA          <  SRR4302532.732760/94‑1 (MQ=60)
                                                                                      CAACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAA         <  SRR4302532.2059523/94‑1 (MQ=60)
                                                                                       AACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAAC        >  SRR4302532.6048002/1‑94 (MQ=60)
                                                                                       AACCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAAC        <  SRR4302532.4996978/94‑1 (MQ=60)
                                                                                         CCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGT      >  SRR4302532.5877323/1‑94 (MQ=60)
                                                                                         CCGCTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGT      <  SRR4302532.4971641/94‑1 (MQ=60)
                                                                                           GCACGTCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTA    <  SRR4302532.3352683/94‑1 (MQ=60)
                                                                                            CTCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAA   <  SRR4302532.5478504/94‑1 (MQ=60)
                                                                                             TCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAA  <  SRR4302532.773576/94‑1 (MQ=60)
                                                                                             TCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAA  >  SRR4302532.2618286/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAACGTATCGCCCTGACTATGCGCCTGGATGAGCAGCCTGGCGAAACCAACGCTCGTCGCGGCGGCGGTAATGAACGCCCGCAAAACAACCGCCCGGCAGCCAAACCACGCGGTCGTGAAGCGCAGCCTGCCGGTAATAGCGCGATGATGGATGCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAA  >  NC_000913/3537159‑3537345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑