Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,548,005	C→T	G701D (GGT→GAT)	malP ←	maltodextrin phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,548,005	0	C	T	99.5%	782.5 / NA	212	G701D (GGT→GAT)	malP	maltodextrin phosphorylase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (99/112); total (99/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 248 total aligned reads displayed.

ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT  >  NC_000913/3547915‑3548094

aTACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCaccttatct                                                                                         <  2:2993677/93‑1 (MQ=255)

 tACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCaccttatctt                                                                                        <  1:4835752/93‑1 (MQ=255)

 tACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCaccttatctt                                                                                        <  1:3771620/93‑1 (MQ=255)

   cTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttc                                                                                      <  2:2730790/93‑1 (MQ=255)

   cTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttc                                                                                      <  2:4835534/93‑1 (MQ=255)

   cTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttc                                                                                      <  1:2598564/93‑1 (MQ=255)

    tgtgCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTttct                                                                                     <  2:2243241/93‑1 (MQ=255)

     gtgCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTAtctttctt                                                                                    >  1:3492395/1‑93 (MQ=255)

       gcagcaTCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTcc                                                                                  >  1:4399665/1‑93 (MQ=255)

       gcagcaTCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTcc                                                                                  >  1:3251384/1‑93 (MQ=255)

         agcaTCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGc                                                                                >  2:2161378/1‑93 (MQ=255)

          gcaTCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGcc                                                                               <  1:3661666/93‑1 (MQ=255)

           caTCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCa                                                                              <  2:5560055/93‑1 (MQ=255)

             tCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAtt                                                                            >  1:3816876/1‑93 (MQ=255)

             tCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAtt                                                                            >  2:4198716/1‑93 (MQ=255)

                ggTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCa                                                                         >  2:3420754/1‑93 (MQ=255)

                 gTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAc                                                                        >  1:4310474/1‑93 (MQ=255)

                 gTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAc                                                                        <  2:5643656/93‑1 (MQ=255)

                 gTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAc                                                                        <  1:3208630/93‑1 (MQ=255)

                 gTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAc                                                                        <  2:2815297/93‑1 (MQ=255)

                   cGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCg                                                                      <  2:5254376/93‑1 (MQ=255)

                    gAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCgg                                                                     >  2:5738085/1‑93 (MQ=255)

                     aaGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCggg                                                                    >  2:896965/1‑93 (MQ=255)

                     aaGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCggg                                                                    >  1:2202152/1‑93 (MQ=255)

                      aGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTGCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGt                                                                   <  1:4975875/93‑1 (MQ=255)

                       ggCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTc                                                                  >  2:3528788/1‑93 (MQ=255)

                        gCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCg                                                                 >  2:2475229/1‑93 (MQ=255)

                         cATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGt                                                                <  1:216222/93‑1 (MQ=255)

                           tGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAg                                                              >  2:5390078/1‑93 (MQ=255)

                           tGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAg                                                              >  1:2043895/1‑93 (MQ=255)

                            gCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGc                                                             <  1:2291349/93‑1 (MQ=255)

                              ttATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCt                                                           >  1:4581198/1‑93 (MQ=255)

                               tATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCtt                                                          <  1:1554812/93‑1 (MQ=255)

                                aTCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCttt                                                         <  2:6060965/93‑1 (MQ=255)

                                aTCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCttt                                                         <  1:4409626/93‑1 (MQ=255)

                                aTCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCttt                                                         <  1:2459235/93‑1 (MQ=255)

                                aTCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCttt                                                         >  2:4658617/1‑93 (MQ=255)

                                 tCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTg                                                        <  1:3575902/93‑1 (MQ=255)

                                 tCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTg                                                        <  1:345856/93‑1 (MQ=255)

                                 tCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTg                                                        <  1:5903318/93‑1 (MQ=255)

                                  cGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTgg                                                       <  1:544648/93‑1 (MQ=255)

                                         gCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGGGTCCAGCCCTTAACCTTCCTTCGGCCATTTCACCGGGTCGTACCTTTTGGCAAGAAt                                                >  2:5178089/1‑93 (MQ=255)

                                           tGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCGTTTCGCCAGAAttc                                              >  1:2721506/1‑92 (MQ=255)

                                            gTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGc                                             <  2:4510363/93‑1 (MQ=255)

                                            gTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGc                                             >  1:1892568/1‑93 (MQ=255)

                                             tATTTACCGCTTGCCAGCTCTTTCAATCCTTCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGcc                                            <  2:3140806/93‑1 (MQ=255)

                                               tttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCtt                                          >  1:1311586/1‑93 (MQ=255)

                                               tttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCtt                                          >  1:2444086/1‑93 (MQ=255)

                                               tttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCtt                                          >  1:4656515/1‑93 (MQ=255)

                                                ttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTc                                         <  1:2884044/93‑1 (MQ=255)

                                                ttACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTc                                         <  2:5595834/93‑1 (MQ=255)

                                                 tACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCa                                        <  2:4426787/93‑1 (MQ=255)

                                                 tACCGCTTCCCAGCTCTTTCAACACTGCGTCCAGCACCTTATCTTTCTCCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCa                                        <  1:3080560/93‑1 (MQ=255)

                                                  aCCGCTTTCCAACTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAc                                       >  1:3686419/1‑93 (MQ=255)

                                                   ccGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACt                                      <  2:3481808/93‑1 (MQ=255)

                                                     gCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTg                                    <  1:651100/93‑1 (MQ=255)

                                                       tttCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTggt                                  >  1:6005173/1‑91 (MQ=255)

                                                        ttCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTc                                 >  2:5946203/1‑93 (MQ=255)

                                                         tCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTcc                                <  2:196254/93‑1 (MQ=255)

                                                         tCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTcc                                <  1:4496229/93‑1 (MQ=255)

                                                            aGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACg                             <  2:4978293/93‑1 (MQ=255)

                                                            aGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACg                             <  1:314910/93‑1 (MQ=255)

                                                             gCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACgg                            >  2:4282070/1‑93 (MQ=255)

                                                             gCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACgg                            <  2:3936091/93‑1 (MQ=255)

                                                             gCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACgg                            <  1:551294/93‑1 (MQ=255)

                                                              ctctTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGt                           >  1:1832861/1‑93 (MQ=255)

                                                              ctctTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGt                           <  2:5873013/93‑1 (MQ=255)

                                                              ctctTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGt                           <  2:3377551/93‑1 (MQ=255)

                                                               tctTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTa                          >  1:1296744/1‑93 (MQ=255)

                                                                ctTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAt                         <  1:4049198/93‑1 (MQ=255)

                                                                ctTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAt                         <  1:5579598/93‑1 (MQ=255)

                                                                  ttCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGa                       <  2:107249/93‑1 (MQ=255)

                                                                   tCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAc                      <  1:1116680/93‑1 (MQ=255)

                                                                   tCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAc                      >  2:1830186/1‑93 (MQ=255)

                                                                    cAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAcc                     >  2:5323901/1‑93 (MQ=255)

                                                                     aaTACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCa                    >  1:4223709/1‑93 (MQ=255)

                                                                      aTACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCaa                   >  1:275124/1‑93 (MQ=255)

                                                                       tACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCaaa                  >  1:1067301/1‑93 (MQ=255)

                                                                        aCTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCaaaa                 <  1:475747/93‑1 (MQ=255)

                                                                         cTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCaaaaa                <  2:3704229/93‑1 (MQ=255)

                                                                         cTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCaaaaa                <  1:767629/93‑1 (MQ=255)

                                                                          tGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAAt               >  2:2692122/1‑93 (MQ=255)

                                                                           gCGTCCAGCCCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATa              <  2:1216114/93‑1 (MQ=255)

                                                                             gTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATaaa            <  1:1695665/93‑1 (MQ=255)

                                                                              tCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAg           >  2:612358/1‑93 (MQ=255)

                                                                              tCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAg           >  2:2602941/1‑93 (MQ=255)

                                                                                cAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAACATAAAGat         >  2:1365965/1‑93 (MQ=255)

                                                                                cAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGat         <  2:2147828/93‑1 (MQ=255)

                                                                                cAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGat         <  1:4003549/93‑1 (MQ=255)

                                                                                 aGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGata        <  2:4104172/93‑1 (MQ=255)

                                                                                   cACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAtt      <  2:2503120/93‑1 (MQ=255)

                                                                                    accttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAttt     <  1:3273501/93‑1 (MQ=255)

                                                                                    accttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAttt     <  2:4711743/93‑1 (MQ=255)

                                                                                    accttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAttt     >  1:1590258/1‑93 (MQ=255)

                                                                                     ccttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAtttt    <  2:2230771/93‑1 (MQ=255)

                                                                                     ccttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAtttt    >  1:5771431/1‑93 (MQ=255)

                                                                                     ccttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATAtttt    >  1:5323941/1‑93 (MQ=255)

                                                                                      cttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTttc   <  1:1206065/93‑1 (MQ=255)

                                                                                      cttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTttc   >  2:72837/1‑93 (MQ=255)

                                                                                       ttatcttTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTttct  <  2:1549161/93‑1 (MQ=255)

ATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCT  >  NC_000913/3547915‑3548094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCAC  >  NC_000913/3547914‑3548098

GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCT                                                                                              <  SRR4302532.5386869/94‑1 (MQ=60)

GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCT                                                                                              <  SRR4302532.6130053/94‑1 (MQ=60)

GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCT                                                                                              <  SRR4302532.4262037/94‑1 (MQ=60)

  TACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTT                                                                                            <  SRR4302532.5002199/94‑1 (MQ=60)

  TACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTT                                                                                            <  SRR4302532.3898596/94‑1 (MQ=60)

  TACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTT                                                                                            <  SRR4302532.494200/94‑1 (MQ=60)

  TACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTT                                                                                            <  SRR4302532.132772/94‑1 (MQ=60)

    CTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCT                                                                                          <  SRR4302532.2685439/94‑1 (MQ=60)

    CTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCT                                                                                          >  SRR4302532.1936452/1‑94 (MQ=60)

     TGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTT                                                                                         >  SRR4302532.3609411/1‑94 (MQ=60)

       TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCC                                                                                       >  SRR4302532.4549410/1‑94 (MQ=60)

       TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCC                                                                                       >  SRR4302532.3359361/1‑94 (MQ=60)

       TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCC                                                                                       <  SRR4302532.4480148/94‑1 (MQ=60)

       TGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCC                                                                                       >  SRR4302532.3359362/1‑94 (MQ=60)

        GCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCG                                                                                      <  SRR4302532.2970923/94‑1 (MQ=60)

          AGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCC                                                                                    >  SRR4302532.1417986/1‑94 (MQ=60)

           GCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCA                                                                                   <  SRR4302532.3784613/94‑1 (MQ=60)

            CATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTATCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAT                                                                                  >  SRR4302532.3727299/1‑94 (MQ=60)

            CATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAT                                                                                  <  SRR4302532.2268911/94‑1 (MQ=60)

            CATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCAT                                                                                  >  SRR4302532.1743445/1‑94 (MQ=60)

             ATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATT                                                                                 >  SRR4302532.3945740/1‑94 (MQ=60)

                 GGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCAC                                                                             >  SRR4302532.4456198/1‑94 (MQ=60)

                  GTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC                                                                            <  SRR4302532.4678945/94‑1 (MQ=60)

                  GTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACC                                                                            <  SRR4302532.3315173/94‑1 (MQ=60)

                    CGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGG                                                                          <  SRR4302532.3821891/94‑1 (MQ=60)

                    CGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGG                                                                          >  SRR4302532.4192663/1‑94 (MQ=60)

                    CGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGG                                                                          <  SRR4302532.5381245/94‑1 (MQ=60)

                     GAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGG                                                                         >  SRR4302532.2275419/1‑94 (MQ=60)

                       AGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCCCCTTATCTTTCTTCCGCCATTTCACCGGGTC                                                                       <  SRR4302532.3228781/94‑1 (MQ=60)

                       AGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTGCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTC                                                                       <  SRR4302532.5146441/94‑1 (MQ=60)

                          CATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTA                                                                    <  SRR4302532.223614/94‑1 (MQ=60)

                           ATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAG                                                                   >  SRR4302532.2111959/1‑94 (MQ=60)

                           ATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAG                                                                   >  SRR4302532.3606091/1‑94 (MQ=60)

                             GCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCC                                                                 <  SRR4302532.2367403/94‑1 (MQ=60)

                              CTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCT                                                                >  SRR4302532.4737401/1‑94 (MQ=60)

                               TTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTT                                                               >  SRR4302532.4268280/1‑94 (MQ=60)

                                TATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTT                                                              <  SRR4302532.1607386/94‑1 (MQ=60)

                                 TTCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTG                                                             <  SRR4302532.1348307/94‑1 (MQ=60)

                                 ATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCCCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTG                                                             <  SRR4302532.2541042/94‑1 (MQ=60)

                                 ATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTG                                                             <  SRR4302532.2541044/94‑1 (MQ=60)

                                 ATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTG                                                             <  SRR4302532.4559843/94‑1 (MQ=60)

                                 ATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTG                                                             <  SRR4302532.3809515/94‑1 (MQ=60)

                                 ATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTCATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTG                                                             <  SRR4302532.2541043/94‑1 (MQ=60)

                                  TCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGG                                                            <  SRR4302532.357828/94‑1 (MQ=60)

                                  TCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGG                                                            <  SRR4302532.6107736/94‑1 (MQ=60)

                                  TCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGG                                                            <  SRR4302532.3695547/94‑1 (MQ=60)

                                   CGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGC                                                           >  SRR4302532.5951126/1‑94 (MQ=60)

                                   CGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGC                                                           <  SRR4302532.563629/94‑1 (MQ=60)

                                   CGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGC                                                           >  SRR4302532.4565404/1‑94 (MQ=60)

                                      CGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAG                                                        >  SRR4302532.3322530/1‑94 (MQ=60)

                                      CGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAG                                                        >  SRR4302532.2723693/1‑94 (MQ=60)

                                       GTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGA                                                       <  SRR4302532.4057943/94‑1 (MQ=60)

                                          GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                    >  SRR4302532.6155521/1‑94 (MQ=60)

                                          GCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATT                                                    <  SRR4302532.5672045/94‑1 (MQ=60)

                                           CTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCGTTTCGCCAGAATTC                                                   >  SRR4302532.2813034/1‑94 (MQ=60)

                                            TGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGC                                                  >  SRR4302532.5493344/1‑94 (MQ=60)

                                            TGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGC                                                  >  SRR4302532.1955318/1‑94 (MQ=60)

                                             GTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCC                                                 <  SRR4302532.1647575/94‑1 (MQ=60)

                                              TAGTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCT                                                <  SRR4302532.5546069/94‑1 (MQ=60)

                                               ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                               >  SRR4302532.4815263/1‑94 (MQ=60)

                                               ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                               >  SRR4302532.1355465/1‑94 (MQ=60)

                                               ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                               >  SRR4302532.6035987/1‑94 (MQ=60)

                                               ATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTT                                               >  SRR4302532.2525357/1‑94 (MQ=60)

                                                 TTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCA                                             <  SRR4302532.2980422/94‑1 (MQ=60)

                                                  TACCGCTTTCCAACTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAC                                            >  SRR4302532.3810205/1‑94 (MQ=60)

                                                  TACCGCTTCCCAGCTCTTTCAACACTGCGTCCAGCACCTTATCTTTCTCCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCAC                                            <  SRR4302532.3182415/94‑1 (MQ=60)

                                                   ACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACT                                           >  SRR4302532.4422599/1‑94 (MQ=60)

                                                   ACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACT                                           <  SRR4302532.3412061/94‑1 (MQ=60)

                                                    CCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTT                                          >  SRR4302532.4521849/1‑94 (MQ=60)

                                                     CGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTT                                         >  SRR4302532.5701068/1‑94 (MQ=60)

                                                     CGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTG                                         <  SRR4302532.3818072/94‑1 (MQ=60)

                                                      GCTTTCCGGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGT                                        <  SRR4302532.279339/94‑1 (MQ=60)

                                                      GCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGT                                        <  SRR4302532.1296389/94‑1 (MQ=60)

                                                      GCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGT                                        <  SRR4302532.673676/94‑1 (MQ=60)

                                                       CTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGGT                                       >  SRR4302532.6212548/1‑94 (MQ=60)

                                                        TTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTC                                      <  SRR4302532.4644717/94‑1 (MQ=60)

                                                        TTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTC                                      >  SRR4302532.3934071/1‑94 (MQ=60)

                                                         TTCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCC                                     >  SRR4302532.2161436/1‑94 (MQ=60)

                                                          TCCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCA                                    <  SRR4302532.4649725/94‑1 (MQ=60)

                                                           CCAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCAC                                   <  SRR4302532.2581316/94‑1 (MQ=60)

                                                            CAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACG                                  <  SRR4302532.5737771/94‑1 (MQ=60)

                                                            CAGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACG                                  <  SRR4302532.1145941/94‑1 (MQ=60)

                                                             AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                 >  SRR4302532.4775049/1‑94 (MQ=60)

                                                             AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                 <  SRR4302532.325839/94‑1 (MQ=60)

                                                             AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                 <  SRR4302532.325840/94‑1 (MQ=60)

                                                             AGCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGG                                 >  SRR4302532.3737865/1‑94 (MQ=60)

                                                              GCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGT                                >  SRR4302532.1893821/1‑94 (MQ=60)

                                                              GCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGT                                >  SRR4302532.283530/1‑94 (MQ=60)

                                                              GCTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGT                                <  SRR4302532.570528/94‑1 (MQ=60)

                                                               CTCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTA                               >  SRR4302532.1340141/1‑94 (MQ=60)

                                                                TCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAT                              >  SRR4302532.4840099/1‑94 (MQ=60)

                                                                TCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAT                              <  SRR4302532.150098/94‑1 (MQ=60)

                                                                TCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAT                              >  SRR4302532.4776573/1‑94 (MQ=60)

                                                                TCTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGACTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTAT                              >  SRR4302532.72925/1‑94 (MQ=60)

                                                                 CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                             <  SRR4302532.5108860/94‑1 (MQ=60)

                                                                 CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                             <  SRR4302532.5773316/94‑1 (MQ=60)

                                                                 CTTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG                             <  SRR4302532.4185475/94‑1 (MQ=60)

                                                                  TTTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGCATG‑A                           >  SRR4302532.647445/1‑94 (MQ=60)

                                                                   TTCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑AC                          <  SRR4302532.6146414/94‑1 (MQ=60)

                                                                    TCAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACC                         <  SRR4302532.1153979/94‑1 (MQ=60)

                                                                     CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCA                        <  SRR4302532.4977084/94‑1 (MQ=60)

                                                                     CAATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCA                        >  SRR4302532.4365451/1‑94 (MQ=60)

                                                                      AATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGAACCA                        >  SRR4302532.284655/1‑94 (MQ=60)

                                                                      AATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAA                       >  SRR4302532.284654/1‑94 (MQ=60)

                                                                       ATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAA                      >  SRR4302532.1103189/1‑94 (MQ=60)

                                                                       ATACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAA                      >  SRR4302532.1794341/1‑94 (MQ=60)

                                                                         ACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAA                    >  SRR4302532.3091047/1‑94 (MQ=60)

                                                                         ACTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAA                    <  SRR4302532.492329/94‑1 (MQ=60)

                                                                          CTGCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAAT                   <  SRR4302532.794121/94‑1 (MQ=60)

                                                                              GTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAG               <  SRR4302532.1752900/94‑1 (MQ=60)

                                                                               TCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGA              >  SRR4302532.1210182/1‑94 (MQ=60)

                                                                               TCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGA              <  SRR4302532.366133/94‑1 (MQ=60)

                                                                               TCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGA              <  SRR4302532.2292809/94‑1 (MQ=60)

                                                                                CCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGAT             <  SRR4302532.3498861/94‑1 (MQ=60)

                                                                                 CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATA            >  SRR4302532.4146389/1‑94 (MQ=60)

                                                                                 CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATA            <  SRR4302532.1954659/94‑1 (MQ=60)

                                                                                 CAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATA            <  SRR4302532.4138424/94‑1 (MQ=60)

                                                                                  AGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATAT           <  SRR4302532.942550/94‑1 (MQ=60)

                                                                                  AGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATAT           <  SRR4302532.232428/94‑1 (MQ=60)

                                                                                  AGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATAT           <  SRR4302532.3252247/94‑1 (MQ=60)

                                                                                    CACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTT         <  SRR4302532.2409298/94‑1 (MQ=60)

                                                                                    CACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTT         >  SRR4302532.1644093/1‑94 (MQ=60)

                                                                                    CACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTT         <  SRR4302532.1975446/94‑1 (MQ=60)

                                                                                     CCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTT        <  SRR4302532.4045853/94‑1 (MQ=60)

                                                                                     CCCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTT        <  SRR4302532.1622364/94‑1 (MQ=60)

                                                                                     ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTT        >  SRR4302532.5972256/1‑94 (MQ=60)

                                                                                     ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTT        <  SRR4302532.3382357/94‑1 (MQ=60)

                                                                                     ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTT        <  SRR4302532.2155661/94‑1 (MQ=60)

                                                                                     ACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTT        >  SRR4302532.5508417/1‑94 (MQ=60)

                                                                                      CCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTC       <  SRR4302532.3315992/94‑1 (MQ=60)

                                                                                       CTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCT      <  SRR4302532.4557455/94‑1 (MQ=60)

                                                                                       CTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCT      <  SRR4302532.1246430/94‑1 (MQ=60)

                                                                                        TTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTT     <  SRR4302532.5812531/94‑1 (MQ=60)

                                                                                         TATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTC    >  SRR4302532.5478147/1‑94 (MQ=60)

                                                                                          ATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCA   >  SRR4302532.271304/1‑94 (MQ=60)

                                                                                          ATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCA   <  SRR4302532.5237888/94‑1 (MQ=60)

                                                                                          ATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCA   >  SRR4302532.3620521/1‑94 (MQ=60)

                                                                                          ATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCA   <  SRR4302532.3790753/94‑1 (MQ=60)

                                                                                           TCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCAC  <  SRR4302532.422552/94‑1 (MQ=60)

GATACTGTGCAGCATCTGGTCGAAGGCATGCTTATCGCCGTCGCTGTATTTACCGCTTTCCAGCTCTTTCAATACTGCGTCCAGCACCTTACCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATG‑ACCAAAAATAAAGATATTTTCTTCAC  >  NC_000913/3547914‑3548098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`