Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,585,834 A→G H339H (CAT→CAC)  ugpC ← ATP‑binding component of sn‑glycerol 3‑phosphate transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,585,8340AG92.5% 611.2 / 28.0 202H339H (CAT→CAC) ugpCATP‑binding component of sn‑glycerol 3‑phosphate transport system
Reads supporting (aligned to +/- strand):  ref base A (7/8);  new base G (93/93);  total (101/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 212 total aligned reads displayed.

TACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATC  >  NC_000913/3585744‑3585924
                                                                                          |                                                                                          
tACTCGTTGTCGTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTg                                                                                          <  1:3774632/93‑1 (MQ=255)
  cTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTgcg                                                                                        >  1:5167726/1‑93 (MQ=255)
   tCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTgcgc                                                                                       >  1:5611958/1‑93 (MQ=255)
      ttGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAg                                                                                    >  2:1558997/1‑93 (MQ=255)
      ttGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAg                                                                                    >  1:1478481/1‑93 (MQ=255)
        gTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGGCAGTc                                                                                  >  2:671362/1‑93 (MQ=255)
        gTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTc                                                                                  <  2:4875323/93‑1 (MQ=255)
           cTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcc                                                                               >  2:1974444/1‑92 (MQ=255)
           cTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGca                                                                               >  2:5085624/1‑93 (MQ=255)
            tGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcac                                                                              >  2:4598445/1‑93 (MQ=255)
             gTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcacc                                                                             >  1:4900299/1‑93 (MQ=255)
             gTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcacc                                                                             >  1:3478873/1‑93 (MQ=255)
              tttCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcaccc                                                                            >  1:121099/1‑92 (MQ=255)
              tttCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcacca                                                                            >  1:2195327/1‑93 (MQ=255)
              tttCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcacca                                                                            >  1:2547716/1‑93 (MQ=255)
              tttCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGcacca                                                                            <  1:2765494/93‑1 (MQ=255)
                tCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCaccacc                                                                          <  1:3097657/93‑1 (MQ=255)
                tCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCaccacc                                                                          >  2:428528/1‑93 (MQ=255)
                 cACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCAccacca                                                                         <  1:1885648/93‑1 (MQ=255)
                 cACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCAccacca                                                                         <  1:106945/93‑1 (MQ=255)
                  aCCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAg                                                                        <  2:5282025/93‑1 (MQ=255)
                    cATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCt                                                                      >  2:335623/1‑93 (MQ=255)
                     aTCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCtt                                                                     >  2:1480118/1‑93 (MQ=255)
                      tCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTc                                                                    >  2:4474413/1‑93 (MQ=255)
                      tCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTc                                                                    <  1:3514907/93‑1 (MQ=255)
                      tCAAAAAGATGCAGATGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTACCGTCGGGCGCATCTGGTGGGCCAGTCGCCCCAGCAGCttg                                                                    >  1:3077941/1‑92 (MQ=255)
                       cAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCt                                                                   >  2:6489/1‑93 (MQ=255)
                       cAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCt                                                                   <  1:2365702/93‑1 (MQ=255)
                       cAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCt                                                                   <  1:1001609/93‑1 (MQ=255)
                         aaaaGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGc                                                                 >  2:2281484/1‑93 (MQ=255)
                         aaaaGATGCAGCTGATGTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGc                                                                 >  1:5491567/1‑93 (MQ=255)
                          aaaGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCt                                                                >  1:3674209/1‑93 (MQ=255)
                          aaaGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCt                                                                <  1:3706293/93‑1 (MQ=255)
                           aaGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTc                                                               >  2:5400200/1‑93 (MQ=255)
                           aaGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTc                                                               >  2:3800580/1‑93 (MQ=255)
                           aaGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTc                                                               <  2:3104808/93‑1 (MQ=255)
                             gATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGc                                                             >  2:2170125/1‑93 (MQ=255)
                               tGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGccc                                                           >  1:1905723/1‑93 (MQ=255)
                               tGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGccc                                                           >  1:3703515/1‑93 (MQ=255)
                                gCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGcccc                                                          <  1:3593003/93‑1 (MQ=255)
                                 cAGATGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCa                                                         <  2:3240804/93‑1 (MQ=255)
                                  aGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAg                                                        <  2:5105426/93‑1 (MQ=255)
                                   gCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGc                                                       <  2:4778905/93‑1 (MQ=255)
                                      gATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTc                                                    >  2:2794825/1‑93 (MQ=255)
                                      gATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTc                                                    >  1:939583/1‑93 (MQ=255)
                                        ttttCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCg                                                  <  1:532564/93‑1 (MQ=255)
                                        ttttCCGCCAGATGCAGCCACACCGTGCTGCCTGCCGTCGGTCGCTCCTGGGGCGCCAGTGGCACAGCCACCTTCCGCTCGCCCCACCGCCCg                                                  >  2:3527862/1‑93 (MQ=255)
                                               ccAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCa                                           >  2:4068098/1‑93 (MQ=255)
                                               ccAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCa                                           >  2:1150651/1‑93 (MQ=255)
                                                cAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAg                                          <  1:4002115/93‑1 (MQ=255)
                                                  gATGCCGCGACAGCGTGCTGCCTGCCGTCGGGCACTCCTGGTGCGCCAGTCGCACCACCAGCTCCTGCTCGCCCCAGCGTCCGTGCGCCAGGt                                        <  1:3654218/93‑1 (MQ=255)
                                                  gATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGt                                        >  1:5332533/1‑93 (MQ=255)
                                                  gATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGt                                        >  2:3629639/1‑93 (MQ=255)
                                                     gCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTAt                                     <  2:5464852/93‑1 (MQ=255)
                                                     gCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTAt                                     <  1:1457544/93‑1 (MQ=255)
                                                      cAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATc                                    >  2:853771/1‑93 (MQ=255)
                                                       aGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCt                                   <  2:2555330/93‑1 (MQ=255)
                                                        gCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTg                                  <  2:4729848/93‑1 (MQ=255)
                                                        gCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTg                                  >  1:4258730/1‑93 (MQ=255)
                                                         ccACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgc                                 <  1:4368712/93‑1 (MQ=255)
                                                         ccACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCACCGTCCGTGCGCCAGGTTATCTgc                                 >  2:908308/1‑93 (MQ=255)
                                                          cacaGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgcg                                <  1:707799/93‑1 (MQ=255)
                                                          cacaGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgcg                                >  1:5681997/1‑93 (MQ=255)
                                                          cacaGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTgcg                                >  1:5056207/1‑93 (MQ=255)
                                                            caGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGcc                              >  2:1101622/1‑93 (MQ=255)
                                                              gCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGa                            >  2:2659839/1‑93 (MQ=255)
                                                              gCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGa                            <  1:879354/93‑1 (MQ=255)
                                                              gCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGa                            <  1:6058605/93‑1 (MQ=255)
                                                                 ttctgcctgcCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGa                         <  2:2775140/91‑1 (MQ=255)
                                                                 tgctgcCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGa                         <  2:2125824/93‑1 (MQ=255)
                                                                 tgctgcCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGa                         <  2:4583226/93‑1 (MQ=255)
                                                                 tgctgcCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGa                         <  2:1637193/93‑1 (MQ=255)
                                                                  gctgcCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGAt                        <  1:1019327/93‑1 (MQ=255)
                                                                  gctgcCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGCAt                        >  1:4800846/1‑93 (MQ=255)
                                                                     gcctgccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGAtctc                     <  1:6123632/93‑1 (MQ=255)
                                                                     gcctgccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGAtctc                     >  2:2930445/1‑93 (MQ=255)
                                                                     gcctgccGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGAtctc                     <  2:2424851/93‑1 (MQ=255)
                                                                      cctgccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTcc                    >  2:3071339/1‑93 (MQ=255)
                                                                      cctgccGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTcc                    <  2:1855047/93‑1 (MQ=255)
                                                                       ctgccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCa                   >  1:5856626/1‑93 (MQ=255)
                                                                        tgcgGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAg                  <  1:5309312/93‑1 (MQ=255)
                                                                        tgccGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTTCAg                  <  2:5601449/93‑1 (MQ=255)
                                                                          ccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAggg                >  1:4663112/1‑91 (MQ=255)
                                                                          ccGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCg                <  1:545657/93‑1 (MQ=255)
                                                                           cGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCgt               <  2:5734763/93‑1 (MQ=255)
                                                                            gTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATTTGCGCCGAGGATCTCCAGCggg              >  1:5646428/1‑91 (MQ=255)
                                                                            gTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCgtg              >  1:2954806/1‑93 (MQ=255)
                                                                               gggCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTcc           <  1:4600184/93‑1 (MQ=255)
                                                                                  ggcTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATc        <  1:2237203/92‑1 (MQ=255)
                                                                                  cgcTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATc        <  1:792596/93‑1 (MQ=255)
                                                                                    cTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCAc      <  2:2055624/93‑1 (MQ=255)
                                                                                    cTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCAc      >  2:1307039/1‑93 (MQ=255)
                                                                                     tCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCAcc     >  2:4176484/1‑93 (MQ=255)
                                                                                      ccTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCa    <  1:4700148/93‑1 (MQ=255)
                                                                                      ccTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCa    <  2:733757/93‑1 (MQ=255)
                                                                                       cTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCCCCAt   >  1:1728415/1‑93 (MQ=255)
                                                                                       cTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCAt   <  1:4444115/93‑1 (MQ=255)
                                                                                       cTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCAt   >  2:5839359/1‑93 (MQ=255)
                                                                                       cTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCAt   <  2:1659401/93‑1 (MQ=255)
                                                                                        tGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATc  <  1:5938449/93‑1 (MQ=255)
                                                                                          |                                                                                          
TACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATC  >  NC_000913/3585744‑3585924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGG  >  NC_000913/3585741‑3585926
                                                                                             |                                                                                            
TCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGG                                                                                              <  SRR4302532.4853322/94‑1 (MQ=60)
TCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGG                                                                                              <  SRR4302532.779754/94‑1 (MQ=60)
 CATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGT                                                                                             >  SRR4302532.3185878/1‑94 (MQ=60)
  ATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTG                                                                                            <  SRR4302532.594445/94‑1 (MQ=60)
  ATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTG                                                                                            <  SRR4302532.594444/94‑1 (MQ=60)
  ATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTG                                                                                            >  SRR4302532.1483655/1‑94 (MQ=60)
   TACTCGTTGTCGTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGC                                                                                           <  SRR4302532.3901728/94‑1 (MQ=60)
   TACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGC                                                                                           >  SRR4302532.1983762/1‑94 (MQ=60)
    ACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCG                                                                                          >  SRR4302532.5345687/1‑94 (MQ=60)
    ACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCG                                                                                          <  SRR4302532.5150236/94‑1 (MQ=60)
     CTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGC                                                                                         >  SRR4302532.859157/1‑94 (MQ=60)
     CTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGC                                                                                         >  SRR4302532.5806711/1‑94 (MQ=60)
        GTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAG                                                                                      >  SRR4302532.1528313/1‑94 (MQ=60)
        GTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAG                                                                                      <  SRR4302532.6232341/94‑1 (MQ=60)
         TTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGT                                                                                     <  SRR4302532.699909/94‑1 (MQ=60)
          TGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTC                                                                                    <  SRR4302532.550939/94‑1 (MQ=60)
               TGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACC                                                                               >  SRR4302532.3595403/1‑94 (MQ=60)
               TGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACC                                                                               <  SRR4302532.4672640/94‑1 (MQ=60)
               TGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACC                                                                               >  SRR4302532.5068697/1‑94 (MQ=60)
                GTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCCGCCACAGCGTGCTGCCTGCCGTCGGGCGGTCCTGGTGAGCCAGGCGCACCC                                                                              >  SRR4302532.125222/1‑94 (MQ=60)
                GTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCC                                                                              >  SRR4302532.125223/1‑94 (MQ=60)
                GTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCA                                                                              >  SRR4302532.2268456/1‑94 (MQ=60)
                GTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCA                                                                              >  SRR4302532.770366/1‑94 (MQ=60)
                GTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCA                                                                              >  SRR4302532.2632680/1‑94 (MQ=60)
                 TTTCACCATTAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCAC                                                                             >  SRR4302532.1957846/1‑94 (MQ=60)
                 TTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCAC                                                                             <  SRR4302532.2858351/94‑1 (MQ=60)
                   TCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCA                                                                           >  SRR4302532.2271072/1‑94 (MQ=60)
                   TCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCA                                                                           <  SRR4302532.3200370/94‑1 (MQ=60)
                    CACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAG                                                                          <  SRR4302532.110631/94‑1 (MQ=60)
                    CACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAG                                                                          <  SRR4302532.1948173/94‑1 (MQ=60)
                       CATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTT                                                                       >  SRR4302532.1803648/1‑94 (MQ=60)
                        ATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTC                                                                      >  SRR4302532.1060050/1‑94 (MQ=60)
                        ATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTC                                                                      >  SRR4302532.3179457/1‑94 (MQ=60)
                         TCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCT                                                                     <  SRR4302532.3632594/94‑1 (MQ=60)
                          CAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTG                                                                    <  SRR4302532.2444610/94‑1 (MQ=60)
                          CAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTG                                                                    <  SRR4302532.4410301/94‑1 (MQ=60)
                          CAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTG                                                                    <  SRR4302532.1035704/94‑1 (MQ=60)
                           AAAAAGATGCAGCTGATGTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGC                                                                   >  SRR4302532.5682004/1‑94 (MQ=60)
                            AAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCT                                                                  >  SRR4302532.3797629/1‑94 (MQ=60)
                            AAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCT                                                                  <  SRR4302532.395837/94‑1 (MQ=60)
                            AAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCT                                                                  >  SRR4302532.3797630/1‑94 (MQ=60)
                             AAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTC                                                                 <  SRR4302532.3830717/94‑1 (MQ=60)
                               AGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGC                                                               >  SRR4302532.4572628/1‑94 (MQ=60)
                                 ATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCC                                                             >  SRR4302532.1968942/1‑94 (MQ=60)
                                 ATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCC                                                             >  SRR4302532.3827839/1‑94 (MQ=60)
                                  TGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCC                                                            <  SRR4302532.5948702/94‑1 (MQ=60)
                                  TGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCC                                                            <  SRR4302532.5585050/94‑1 (MQ=60)
                                   GCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCA                                                           <  SRR4302532.3713167/94‑1 (MQ=60)
                                    cacccGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCCCTCCTGGTGCCCCAGTCGCACCACCAGCTTCTGCTCGCCCCAG                                                          <  SRR4302532.2037816/89‑1 (MQ=60)
                                    GAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAG                                                          <  SRR4302532.5846538/94‑1 (MQ=60)
                                    CAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAG                                                          <  SRR4302532.3829936/94‑1 (MQ=60)
                                     AGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGC                                                         <  SRR4302532.2448370/94‑1 (MQ=60)
                                     AGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGC                                                         <  SRR4302532.5813224/94‑1 (MQ=60)
                                     AGCCGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGC                                                         >  SRR4302532.3193375/1‑94 (MQ=60)
                                      GCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCTCAGCG                                                        >  SRR4302532.3779704/1‑94 (MQ=60)
                                      GCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCG                                                        <  SRR4302532.5308820/94‑1 (MQ=60)
                                        TGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTC                                                      >  SRR4302532.971784/1‑94 (MQ=60)
                                        TGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTC                                                      >  SRR4302532.3704037/1‑94 (MQ=60)
                                        TGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTC                                                      <  SRR4302532.6082468/94‑1 (MQ=60)
                                         GATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCC                                                     >  SRR4302532.2908386/1‑94 (MQ=60)
                                           TTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGT                                                   <  SRR4302532.2425949/94‑1 (MQ=60)
                                           TTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGT                                                   <  SRR4302532.551138/94‑1 (MQ=60)
                                           TTTACCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCCCCAGCTTCTGCTCGCCCCAGCGTCCGT                                                   <  SRR4302532.4934089/94‑1 (MQ=60)
                                              TCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCTCCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCG                                                <  SRR4302532.481097/94‑1 (MQ=60)
                                              TCCGCCAGATGCAGCCACAGCGGGCTGCCTGCCGTCGGGCGCTCCTGGTGAGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCG                                                >  SRR4302532.5371174/1‑94 (MQ=60)
                                                  CCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAG                                            <  SRR4302532.3884438/94‑1 (MQ=60)
                                                  CCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAG                                            >  SRR4302532.2666051/1‑94 (MQ=60)
                                                   CAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGG                                           <  SRR4302532.4136939/94‑1 (MQ=60)
                                                    AGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGT                                          >  SRR4302532.5517459/1‑94 (MQ=60)
                                                     GATGCCGCGACAGCGTGCTGCCTGCCGTCGGGCACTCCTGGTGCGCCAGTCGCACCACCAGCTCCTGCTCGCCCCAGCGTCCGTGCGCCAGGTT                                         <  SRR4302532.3776885/94‑1 (MQ=60)
                                                       agcagccccggcgtcctcCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCGCCGCCAGCTTCTCCTCCCCCCAGCGTCCGTGCGCCAGGTTAT                                       <  SRR4302532.3942760/76‑1 (MQ=60)
                                                        GCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATC                                      <  SRR4302532.1506679/94‑1 (MQ=60)
                                                          AGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTG                                    <  SRR4302532.3302846/94‑1 (MQ=60)
                                                          AGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTG                                    <  SRR4302532.6298112/94‑1 (MQ=60)
                                                          AGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTG                                    >  SRR4302532.4401997/1‑94 (MQ=60)
                                                            CCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCG                                  >  SRR4302532.5880361/1‑94 (MQ=60)
                                                            CCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCG                                  <  SRR4302532.4516910/94‑1 (MQ=60)
                                                            CCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCG                                  >  SRR4302532.5229749/1‑94 (MQ=60)
                                                            CCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCG                                  >  SRR4302532.5058801/1‑94 (MQ=60)
                                                             CACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGC                                 <  SRR4302532.731983/94‑1 (MQ=60)
                                                                 GCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAG                             <  SRR4302532.909424/94‑1 (MQ=60)
                                                                 GCGTGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAG                             <  SRR4302532.6267798/94‑1 (MQ=60)
                                                                  CGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCCGCGTCCGTGCGCCAGGTTATCTGCGCCGAGG                            >  SRR4302532.1516813/1‑94 (MQ=60)
                                                                   GTGCTGCCTGCCGTCGGGTGCTCCTGGTGCGTCAGTCGCACCACCTGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGGTATCTGCGCCGAGGT                           >  SRR4302532.5824472/1‑94 (MQ=60)
                                                                    TGCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGCAT                          >  SRR4302532.4966183/1‑94 (MQ=60)
                                                                     GCTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATC                         <  SRR4302532.1053904/94‑1 (MQ=60)
                                                                      CTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCT                        >  SRR4302532.4376513/1‑94 (MQ=60)
                                                                      CTGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCT                        <  SRR4302532.3942574/94‑1 (MQ=60)
                                                                       TGCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCGGCGTCCGTGCGCCAGGTTATCTGCGCAGAGGATCTC                       >  SRR4302532.5168067/1‑94 (MQ=60)
                                                                        GCCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCC                      <  SRR4302532.6334950/94‑1 (MQ=60)
                                                                         CCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCA                     <  SRR4302532.2044644/94‑1 (MQ=60)
                                                                         CCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCA                     >  SRR4302532.6059713/1‑94 (MQ=60)
                                                                         CCTGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCA                     <  SRR4302532.1685994/94‑1 (MQ=60)
                                                                          CTGCCGTCGGGCGCTCCTGGTGCACCTGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAG                    <  SRR4302532.3599123/94‑1 (MQ=60)
                                                                           TGCGGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGC                   <  SRR4302532.5492904/94‑1 (MQ=60)
                                                                           TGCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGC                   >  SRR4302532.10481/1‑94 (MQ=60)
                                                                            GCCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGGG                  >  SRR4302532.4822105/1‑94 (MQ=60)
                                                                             CCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGT                 <  SRR4302532.564667/94‑1 (MQ=60)
                                                                             CCGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGT                 >  SRR4302532.5446939/1‑94 (MQ=60)
                                                                              CGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATTTGCGCCGAGGATCTCCAGCGGG                >  SRR4302532.5842817/1‑94 (MQ=60)
                                                                              CGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTG                >  SRR4302532.3052730/1‑94 (MQ=60)
                                                                              CGTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTG                <  SRR4302532.342909/94‑1 (MQ=60)
                                                                              CGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGGGCCGAGGATCTCCAGCGTG                >  SRR4302532.957076/1‑94 (MQ=60)
                                                                               GTCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGT               >  SRR4302532.4839364/1‑94 (MQ=60)
                                                                                TCGGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTC              >  SRR4302532.5791937/1‑94 (MQ=60)
                                                                                  GGGCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCA            <  SRR4302532.4757020/94‑1 (MQ=60)
                                                                                    GCGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATC          <  SRR4302532.3727915/94‑1 (MQ=60)
                                                                                     GGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCA         <  SRR4302532.2311436/94‑1 (MQ=60)
                                                                                     CGCTCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCA         <  SRR4302532.819721/94‑1 (MQ=60)
                                                                                        TCCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCA      >  SRR4302532.872456/1‑94 (MQ=60)
                                                                                         CCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCCCCAT     >  SRR4302532.1786607/1‑94 (MQ=60)
                                                                                         CCTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCAT     <  SRR4302532.4860483/94‑1 (MQ=60)
                                                                                         CCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCAT     <  SRR4302532.5654616/94‑1 (MQ=60)
                                                                                          CTGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATC    <  SRR4302532.4595568/94‑1 (MQ=60)
                                                                                          CTGGTGCGCCAGGCCCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATC    <  SRR4302532.5514567/94‑1 (MQ=60)
                                                                                           TGGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCG   <  SRR4302532.5229967/94‑1 (MQ=60)
                                                                                           TGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCG   <  SRR4302532.6143951/94‑1 (MQ=60)
                                                                                            GGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGG  <  SRR4302532.787220/94‑1 (MQ=60)
                                                                                            GGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGG  <  SRR4302532.6056725/94‑1 (MQ=60)
                                                                                            GGTGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGG  <  SRR4302532.3053599/94‑1 (MQ=60)
                                                                                             |                                                                                            
TCATACTCGTTGTCCTGTTTCACCATCAAAAAGATGCAGCTGATTTTCCGCCAGATGCAGCCACAGCGTGCTGCCTGCCGTCGGGCGCTCCTGATGCGCCAGTCGCACCACCAGCTTCTGCTCGCCCCAGCGTCCGTGCGCCAGGTTATCTGCGCCGAGGATCTCCAGCGTGTCCATCACCATCGG  >  NC_000913/3585741‑3585926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑