Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,627,1401.G100.0% 847.0 / NA 233intergenic (‑22/+25)yhiH/yhiIputative ATP‑binding component of a transport system, fragment 1/putative membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (127/106);  total (127/106)

Only 100 of 272 total aligned reads displayed.

ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227
                                                                                             |                                                                                        
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                         <  2:43773/93‑1 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gg                                                                                        >  2:2992450/1‑93 (MQ=255)
   aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggg                                                                                       >  1:5673732/1‑92 (MQ=255)
   aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggg                                                                                       >  2:2346477/1‑92 (MQ=255)
     tcAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggac                                                                                     <  2:1945609/92‑4 (MQ=255)
     aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggac                                                                                     >  1:1972589/1‑90 (MQ=255)
     aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggac                                                                                     >  1:2764522/1‑90 (MQ=255)
     aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggac                                                                                     <  1:2335704/93‑4 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACggg                                                                                  >  2:2678954/1‑93 (MQ=255)
          gggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGaa                                                                                >  2:5167039/1‑93 (MQ=255)
            gCCGGAATATCGAGAGTAATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCGCGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAAcc                                                                              <  1:1849683/93‑1 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGtt                                                                          >  1:5093265/1‑93 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTcc                                                                        <  2:3928111/93‑1 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTcc                                                                        <  2:4008618/93‑1 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCa                                                                       >  1:450534/1‑93 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCa                                                                       >  2:704359/1‑93 (MQ=255)
                    atCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGGACCAGTTCCAg                                                                      >  1:3701649/1‑93 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGa                                                                     <  2:4868361/93‑1 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTAACGCCCGCCAGTTGCGCGACAGGCGGG‑CCGGGAACCAGTTcccgc                                                                     <  1:5489765/93‑4 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGAt                                                                    >  1:6139067/1‑93 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATg                                                                   >  2:4543669/1‑93 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATg                                                                   >  1:4961626/1‑93 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATg                                                                   >  2:4980808/1‑93 (MQ=255)
                        agagTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGc                                                                  >  1:5190193/1‑93 (MQ=255)
                           gtaatatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTc                                                               <  1:2239756/90‑1 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTc                                                               <  1:3389852/93‑1 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTc                                                               >  1:1573683/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTc                                                               >  1:6067389/1‑93 (MQ=255)
                            tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCa                                                              <  2:5060634/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGTTTTTCCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAtt                                                            <  1:252483/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGtactt                                                            >  1:4913192/1‑89 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAtt                                                            >  2:4605645/1‑93 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCAtt                                                            <  1:2610497/93‑1 (MQ=255)
                               tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTg                                                           <  1:1809463/93‑1 (MQ=255)
                               tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTg                                                           <  1:1351592/93‑1 (MQ=255)
                               tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTg                                                           >  1:1417600/1‑93 (MQ=255)
                                atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGc                                                          <  2:3695729/93‑1 (MQ=255)
                                atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGc                                                          <  2:2423688/93‑1 (MQ=255)
                                atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGCTGCGCGACAGGCGGG‑ACGGGGACCAGGTTCAGATGCGTCATTGc                                                          >  2:146348/1‑93 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         <  1:4340579/93‑1 (MQ=255)
                                  tGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑GCGGGAACCAGTTCCAGATGCGTCATTGCgg                                                        >  2:116203/1‑93 (MQ=255)
                                  tGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCgg                                                        <  1:2481010/93‑1 (MQ=255)
                                   gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCCGTTCCAGATGCGTCATTGCgca                                                       >  2:5814557/1‑91 (MQ=255)
                                   gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                       >  1:4674956/1‑93 (MQ=255)
                                   gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                       >  1:803415/1‑93 (MQ=255)
                                    ttCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCa                                                      >  2:1365173/1‑93 (MQ=255)
                                      cAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAc                                                    <  2:1622470/93‑1 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑GCGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   >  2:2227193/1‑93 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   <  2:6087575/93‑1 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   <  1:2197824/93‑1 (MQ=255)
                                         cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTc                                                 <  2:1087733/93‑1 (MQ=255)
                                          gcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTca                                                <  2:4915122/93‑1 (MQ=255)
                                            aaCTGTTTTTCCATAATGCTTGCTCACGCCCTCCAGTTGTGCTACTGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCTCTGCGGCAACCTctcc                                              >  2:5481273/1‑93 (MQ=255)
                                            aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATCGCGGCAACCTcacc                                              <  1:5260127/93‑1 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccc                                             >  2:5360604/1‑92 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             >  1:1735378/1‑93 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            <  2:4759561/93‑1 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            <  2:4295733/93‑1 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            <  2:2108931/93‑1 (MQ=255)
                                                gTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                          <  1:358885/93‑1 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACTCCCGCCAGTTGCGCGACAGGC‑GGAACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACgtg                                         <  1:3252354/93‑3 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         >  2:2215261/1‑93 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         >  2:3088471/1‑93 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgt                                       <  1:2635215/93‑1 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgt                                       <  2:3847178/93‑1 (MQ=255)
                                                       cATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTTCGGCAACCTCACCACGAGgtcgtc                                   >  1:2042258/1‑93 (MQ=255)
                                                       cATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtc                                   <  1:3912388/93‑1 (MQ=255)
                                                       cATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgccgtc                                   >  2:1683286/1‑93 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGGGgccgtcg                                  >  1:3016642/1‑92 (MQ=255)
                                                        aTAATGCGGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  <  1:10179/93‑1 (MQ=255)
                                                         tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 <  2:3034533/93‑1 (MQ=255)
                                                         tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 >  2:2829726/1‑93 (MQ=255)
                                                         tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 >  2:1810589/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               >  2:66421/1‑93 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              <  2:3894110/93‑1 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              >  2:1381238/1‑93 (MQ=255)
                                                                 gCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGa                         <  2:3533250/93‑1 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        >  2:3429831/1‑93 (MQ=255)
                                                                   tCACGCCCACCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       <  2:5755350/93‑1 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      <  1:3606947/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCGCCACGAGGTCGTCAGGCCACGGAAGttc                    <  1:2876017/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    <  2:3393635/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    <  1:5343540/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    <  1:1209111/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    >  1:4332148/1‑93 (MQ=255)
                                                                         ccgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttcttc                 <  2:4144236/93‑1 (MQ=255)
                                                                          cgccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                <  1:4018899/93‑1 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               <  1:2138200/93‑1 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              >  2:910286/1‑93 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              >  2:822696/1‑93 (MQ=255)
                                                                               gTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  1:588376/1‑93 (MQ=255)
                                                                               gTTGCGCGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  1:1047167/1‑93 (MQ=255)
                                                                                  gcgcGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAcccc        >  2:1335953/1‑92 (MQ=255)
                                                                                  gcgcGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg        >  1:854060/1‑93 (MQ=255)
                                                                                  gcgcGACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg        >  2:5957916/1‑93 (MQ=255)
                                                                                     cgACAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAc     <  1:4798701/93‑1 (MQ=255)
                                                                                       aCAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc   >  2:3237033/1‑93 (MQ=255)
                                                                                       aCAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc   >  1:3559881/1‑93 (MQ=255)
                                                                                       aCAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACAACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc   >  1:3316986/1‑93 (MQ=255)
                                                                                        cAGGCGGG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCa  >  1:4266091/1‑93 (MQ=255)
                                                                                             |                                                                                        
ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGC  >  NC_000913/3627049‑3627235
                                                                                              |                                                                                               
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                                 >  SRR4302532.2763834/1‑94 (MQ=60)
CCAGACAGCGGGCCGGAATATCGAGAGTGATATTGTTCATCGCAACGGTTTTTCCATAATGCTGGCTCACCCCCGCCAGTTGCGCGACAGGC‑GG                                                                                                 <  SRR4302532.4040689/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGTTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑‑G                                                                                              <  SRR4302532.4479962/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑‑G                                                                                              >  SRR4302532.5871697/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑‑G                                                                                              >  SRR4302532.3407466/1‑94 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                               <  SRR4302532.5430319/94‑3 (MQ=60)
   TGCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                              <  SRR4302532.5088861/94‑4 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                              <  SRR4302532.4743216/94‑4 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                              >  SRR4302532.2038110/1‑91 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                              >  SRR4302532.2857346/1‑91 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACG                                                                                            >  SRR4302532.3000801/1‑94 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACG                                                                                            <  SRR4302532.1350013/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACG                                                                                            <  SRR4302532.2413513/94‑1 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGG                                                                                           <  SRR4302532.693104/94‑1 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGG                                                                                          >  SRR4302532.6209267/1‑94 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAA                                                                                        >  SRR4302532.5268050/1‑94 (MQ=60)
           GCCGGAATATCGAGAGTAATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCGCGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCA                                                                                     <  SRR4302532.1911073/94‑1 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAG                                                                                    <  SRR4302532.67626/94‑1 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAG                                                                                    >  SRR4302532.2600055/1‑94 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACTAGT                                                                                   >  SRR4302532.3462013/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTT                                                                                  >  SRR4302532.5268409/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTT                                                                                  <  SRR4302532.2131068/94‑1 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTT                                                                                  >  SRR4302532.4400803/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTT                                                                                  <  SRR4302532.4728986/94‑1 (MQ=60)
               GAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTC                                                                                 <  SRR4302532.615111/94‑1 (MQ=60)
               GAAGATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTC                                                                                 <  SRR4302532.495889/94‑1 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCC                                                                                <  SRR4302532.5136779/94‑1 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGCTCC                                                                                >  SRR4302532.2295556/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCA                                                                               >  SRR4302532.466183/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCA                                                                               >  SRR4302532.2103835/1‑94 (MQ=60)
                  TATCGAGAGTGCCATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAG                                                                              <  SRR4302532.5103307/94‑1 (MQ=60)
                  TATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGGACCAGTTCCAG                                                                              >  SRR4302532.3825924/1‑94 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGA                                                                             <  SRR4302532.2932081/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGAT                                                                            <  SRR4302532.534934/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGAT                                                                            >  SRR4302532.6350842/1‑94 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTAACGCCCGCCAGTTGCGCGACAGGCGGG‑‑CCGGGAACCAGTTCCcgct                                                                            <  SRR4302532.5680141/94‑5 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATG                                                                           >  SRR4302532.2218730/1‑94 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATG                                                                           >  SRR4302532.5131795/1‑94 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATG                                                                           >  SRR4302532.1582903/1‑94 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATG                                                                           <  SRR4302532.2095807/94‑1 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATG                                                                           <  SRR4302532.583886/94‑1 (MQ=60)
                      GAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGC                                                                          >  SRR4302532.5369066/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCG                                                                         >  SRR4302532.1656581/1‑94 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGT                                                                        >  SRR4302532.6021754/1‑94 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGT                                                                        >  SRR4302532.502144/1‑94 (MQ=60)
                         AGTGATATTGTTGAGCGCAACGGTGTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTC                                                                       <  SRR4302532.1983104/94‑1 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTC                                                                       >  SRR4302532.1626956/1‑94 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTC                                                                       <  SRR4302532.4100170/94‑1 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTC                                                                       >  SRR4302532.6276931/1‑94 (MQ=60)
                          GTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCA                                                                      <  SRR4302532.3503235/94‑1 (MQ=60)
                          GTAATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCA                                                                      <  SRR4302532.2314077/94‑1 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCAT                                                                     >  SRR4302532.2291561/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTTGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGATCCAGCTGCGTCATT                                                                    >  SRR4302532.1740128/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTactt                                                                    >  SRR4302532.5081999/1‑90 (MQ=60)
                             ATATTGTTCAGCGCAACGTTTTTCCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302532.261262/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302532.1873431/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                   >  SRR4302532.2036748/1‑94 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                   >  SRR4302532.1465169/1‑94 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302532.2697820/94‑1 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGC                                                                  <  SRR4302532.1396789/94‑1 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGC                                                                  <  SRR4302532.1869821/94‑1 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGC                                                                  <  SRR4302532.3690292/94‑1 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                 >  SRR4302532.4143679/1‑94 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGG                                                                <  SRR4302532.4487193/94‑1 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGC                                                               >  SRR4302532.4834359/1‑94 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGC                                                               >  SRR4302532.830853/1‑94 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGC                                                               <  SRR4302532.2563674/94‑1 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                              >  SRR4302532.2395820/1‑94 (MQ=60)
                                   TTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAC                                                             >  SRR4302532.893770/1‑94 (MQ=60)
                                    TCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAC                                                            <  SRR4302532.1839067/94‑1 (MQ=60)
                                     CAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGAAACCAGTTCCAGAACAGCCATTGCGGCAACC                                                           >  SRR4302532.3515293/1‑94 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCT                                                          <  SRR4302532.2271010/94‑1 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGGCAGTTGCGCGACAGGC‑GGGTCGGGGAACC‑GATCCAGATGCGTCATTGCGGCAAGCTCAC                                                       >  SRR4302532.5040334/1‑94 (MQ=60)
                                         CCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                       <  SRR4302532.3532176/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      <  SRR4302532.1592364/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      <  SRR4302532.3323475/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      >  SRR4302532.5028303/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      >  SRR4302532.4372534/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                     >  SRR4302532.2704621/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                     >  SRR4302532.1793786/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATCGCGGCAACCTCACCA                                                     <  SRR4302532.5441510/94‑1 (MQ=60)
                                            AGGGTTTTTCCATAATACTGACTCCCGCCCGGCAGTTGAGCGACAGCCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCCC                                                    <  SRR4302532.2727528/94‑1 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                    <  SRR4302532.1315042/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                                 >  SRR4302532.124483/1‑94 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                                 <  SRR4302532.371320/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACTCCCGCCAGTTGCGCGACAGGC‑GGA‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGTGG                                                <  SRR4302532.3360396/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                <  SRR4302532.5726070/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                <  SRR4302532.3921911/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                <  SRR4302532.3719809/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                <  SRR4302532.1819745/94‑1 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGT                                               <  SRR4302532.5451065/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                              <  SRR4302532.2723511/94‑1 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTTCGGCAACCTCACCACGAGGTCGTC                                           >  SRR4302532.2110275/1‑94 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGgggccgtcg                                          >  SRR4302532.3116187/1‑85 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                          <  SRR4302532.4044266/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                          >  SRR4302532.303292/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                         >  SRR4302532.1191038/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAAACAGTTCCAGATGCGTCATTGCGGTAACCTCACCACGAGGGCGTCAG                                         >  SRR4302532.963607/1‑94 (MQ=60)
                                                       ATAATGCGGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                         <  SRR4302532.10614/94‑1 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGGTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        >  SRR4302532.3753081/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                       >  SRR4302532.2752037/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                   >  SRR4302532.6004451/1‑94 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                   >  SRR4302532.2367579/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCCCCACGAGGTCGTCAGGCCACGG                                  >  SRR4302532.3710871/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCCCGG                                  >  SRR4302532.4590467/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGG                                  >  SRR4302532.6287487/1‑94 (MQ=60)
                                                               GGCTCACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGCCGTCAGGCCACGGA                                 >  SRR4302532.5498199/1‑94 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                             <  SRR4302532.3727516/94‑1 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                             <  SRR4302532.3475088/94‑1 (MQ=60)
                                                                    ACGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTC                            >  SRR4302532.4478498/1‑94 (MQ=60)
                                                                     CGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCGCCACGAGGTCGTCAGGCCACGGAAGTTCT                           <  SRR4302532.2972188/94‑1 (MQ=60)
                                                                     CGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCT                           <  SRR4302532.1249567/94‑1 (MQ=60)
                                                                     CGCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCT                           <  SRR4302532.5528926/94‑1 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                          <  SRR4302532.848094/94‑1 (MQ=60)
                                                                        CCGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCA                        >  SRR4302532.4521758/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                       <  SRR4302532.4154328/94‑1 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                       >  SRR4302532.855024/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAGGGAAGTTCTTCATT                      >  SRR4302532.2237517/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                      >  SRR4302532.5613894/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                      <  SRR4302532.2209606/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                    <  SRR4302532.2662214/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                    <  SRR4302532.4431654/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                    <  SRR4302532.543360/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                    <  SRR4302532.29093/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   >  SRR4302532.2678360/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   <  SRR4302532.1066402/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   >  SRR4302532.608900/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   >  SRR4302532.1082468/1‑94 (MQ=60)
                                                                              GTTGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACC                  <  SRR4302532.4457167/94‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG                >  SRR4302532.883419/1‑94 (MQ=60)
                                                                                   GCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAC             >  SRR4302532.1440345/1‑94 (MQ=60)
                                                                                   GCGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAC             <  SRR4302532.2536386/94‑1 (MQ=60)
                                                                                    CGACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC            <  SRR4302532.4963974/94‑1 (MQ=60)
                                                                                     GACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC           >  SRR4302532.3678997/1‑94 (MQ=60)
                                                                                     GACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACAACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC           >  SRR4302532.3427587/1‑94 (MQ=60)
                                                                                      ACAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA          >  SRR4302532.4409693/1‑94 (MQ=60)
                                                                                       CAGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC         >  SRR4302532.6326910/1‑94 (MQ=60)
                                                                                        AGGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACG        >  SRR4302532.1042512/1‑94 (MQ=60)
                                                                                         GGCGGG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGC       <  SRR4302532.4074607/94‑1 (MQ=60)
                                                                                           G‑GG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC    <  SRR4302532.3855347/94‑1 (MQ=60)
                                                                                              G‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGC  <  SRR4302532.1202028/94‑1 (MQ=60)
                                                                                              |                                                                                               
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG‑‑ACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGC  >  NC_000913/3627049‑3627235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑