Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,634,770 T→C I91V (ATC→GTC)  yhiN ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,634,7700TC87.3% 718.1 / 88.1 268I91V (ATC→GTC) yhiNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (14/20);  new base C (93/141);  total (107/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

Only 100 of 272 total aligned reads displayed.

CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGC  >  NC_000913/3634679‑3634860
                                                                                           |                                                                                          
 ccTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGc                                                                                          >  1:86856/1‑93 (MQ=255)
 ccTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGc                                                                                          >  2:5216117/1‑93 (MQ=255)
 ccTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGc                                                                                          <  2:3964924/93‑1 (MQ=255)
 ccTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGc                                                                                          >  1:1717704/1‑93 (MQ=255)
   ttCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGATGCCCTAACGTTTTCTCGTGCCAGGCGACGCCg                                                                                        >  2:863153/1‑93 (MQ=255)
     cccGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCgtg                                                                                      <  1:4271347/91‑1 (MQ=255)
      tcGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCgtgt                                                                                     <  2:5190053/93‑1 (MQ=255)
      tcGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTGTTCTCGTGCCAGGCGATGCCgtgt                                                                                     <  1:2807629/93‑1 (MQ=255)
       cGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGtt                                                                                    <  2:2580838/93‑1 (MQ=255)
        gCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGttt                                                                                   >  2:3737620/1‑93 (MQ=255)
        gCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGttt                                                                                   >  1:5407771/1‑93 (MQ=255)
         cACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTa                                                                                  <  2:6541685/93‑1 (MQ=255)
           cTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTAtt                                                                                <  2:6278861/93‑1 (MQ=255)
           cTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTAtt                                                                                >  2:4943324/1‑93 (MQ=255)
            tCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTg                                                                               <  2:4093921/93‑1 (MQ=255)
             cATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGa                                                                              >  2:3349615/1‑93 (MQ=255)
             cATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGa                                                                              <  2:4522688/93‑1 (MQ=255)
              aTCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGAc                                                                             <  1:3255217/93‑1 (MQ=255)
               tCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGAcc                                                                            >  2:2092052/1‑93 (MQ=255)
               tCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGAcc                                                                            >  2:4263089/1‑93 (MQ=255)
                ccaccaGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCa                                                                           <  2:6470455/93‑1 (MQ=255)
                 caccaGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAg                                                                          <  1:414408/93‑1 (MQ=255)
                  accaGCATGTCGATAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGa                                                                         <  2:161737/93‑1 (MQ=255)
                  accaGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGa                                                                         <  2:6619859/93‑1 (MQ=255)
                    caGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATc                                                                       <  1:2182042/93‑1 (MQ=255)
                     aGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCa                                                                      >  1:2523313/1‑93 (MQ=255)
                      gCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCaa                                                                     >  2:5649401/1‑93 (MQ=255)
                       cATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAAt                                                                    <  2:936110/93‑1 (MQ=255)
                       cATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAAt                                                                    >  1:3389838/1‑93 (MQ=255)
                        aTGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATg                                                                   >  1:2043096/1‑93 (MQ=255)
                        aTGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATg                                                                   <  1:1247660/93‑1 (MQ=255)
                         tGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGa                                                                  >  1:2094066/1‑93 (MQ=255)
                         tGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGa                                                                  <  2:2299641/93‑1 (MQ=255)
                           tCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGaaa                                                                >  1:4809910/1‑93 (MQ=255)
                           tCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGaaa                                                                <  2:2805813/93‑1 (MQ=255)
                           tCGACAATATGCGGCGCGGAGTCATCGCAGAAGAGGTGCCCTAACGTATTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGata                                                                <  1:5355674/93‑3 (MQ=255)
                            cGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAAt                                                               >  2:1108021/1‑93 (MQ=255)
                              aCAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATcc                                                             <  1:4559512/93‑1 (MQ=255)
                              aCAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATcc                                                             <  2:5276400/93‑1 (MQ=255)
                              aCAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATcc                                                             <  1:1811924/93‑1 (MQ=255)
                              aCAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATcc                                                             <  2:2907719/93‑1 (MQ=255)
                               cAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATccc                                                            >  1:263745/1‑93 (MQ=255)
                               cAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATccc                                                            <  2:4456215/93‑1 (MQ=255)
                               cAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATccc                                                            <  2:4963786/93‑1 (MQ=255)
                                aaTCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCa                                                           <  1:4881073/93‑1 (MQ=255)
                                 aTCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCAc                                                          >  1:621600/1‑93 (MQ=255)
                                  tCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACt                                                         >  1:3604049/1‑93 (MQ=255)
                                  tCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACt                                                         >  2:4773680/1‑93 (MQ=255)
                                    tgctgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTgg                                                       <  2:6311285/93‑1 (MQ=255)
                                     gctgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTggg                                                      >  2:3230229/1‑93 (MQ=255)
                                     gctgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTggg                                                      <  1:5674675/93‑1 (MQ=255)
                                       tgcGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTa                                                    >  1:1097038/1‑93 (MQ=255)
                                        gcgcGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTaa                                                   >  2:2758496/1‑93 (MQ=255)
                                         cgcgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTaaa                                                  >  1:420291/1‑93 (MQ=255)
                                          gcgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTaaaa                                                 <  1:3989301/93‑1 (MQ=255)
                                          gcgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTaaaa                                                 >  1:835621/1‑93 (MQ=255)
                                           cgGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTTACCAGATCAATGAAATCCCACTGGGTAAAAc                                                >  1:778266/1‑93 (MQ=255)
                                            ggAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACg                                               >  1:153712/1‑93 (MQ=255)
                                            ggAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACg                                               <  1:1360773/93‑1 (MQ=255)
                                             gAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGt                                              >  1:3960440/1‑93 (MQ=255)
                                             gAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGt                                              >  1:4708249/1‑93 (MQ=255)
                                             gAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGt                                              >  2:1482533/1‑93 (MQ=255)
                                              aGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTg                                             <  1:5989916/93‑1 (MQ=255)
                                              aGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTg                                             <  1:4240402/93‑1 (MQ=255)
                                                tCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCg                                           >  1:1187390/1‑93 (MQ=255)
                                                tCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCg                                           <  2:4678018/93‑1 (MQ=255)
                                                   tCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGt                                        <  1:174640/93‑1 (MQ=255)
                                                   tCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGt                                        <  1:6630745/93‑1 (MQ=255)
                                                    cGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTg                                       <  2:1321692/93‑1 (MQ=255)
                                                      cAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCa                                     <  1:5819038/93‑1 (MQ=255)
                                                      cAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCa                                     >  2:180980/1‑93 (MQ=255)
                                                       agaagaGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAg                                    <  1:2469241/93‑1 (MQ=255)
                                                              ttGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACa                             <  2:472904/93‑1 (MQ=255)
                                                               tGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACaa                            <  1:2759651/93‑1 (MQ=255)
                                                                  ccTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTTAAACGTGCGAGTGCAGACTTACaaaaa                         >  1:6070743/1‑93 (MQ=255)
                                                                  ccTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACaaaaa                         <  2:218558/93‑1 (MQ=255)
                                                                  ccTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACaaaaa                         >  1:4992141/1‑93 (MQ=255)
                                                                   cTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAAt                        >  1:3771107/1‑93 (MQ=255)
                                                                   cTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAAt                        <  2:3425960/93‑1 (MQ=255)
                                                                   cTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAAt                        <  1:5114873/93‑1 (MQ=255)
                                                                   cTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAAt                        <  2:1176556/93‑1 (MQ=255)
                                                                    tAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATg                       >  2:5083582/1‑93 (MQ=255)
                                                                    tAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATg                       >  1:4316493/1‑93 (MQ=255)
                                                                      aCGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCg                     <  1:3149025/93‑1 (MQ=255)
                                                                      aCGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCg                     <  1:2335273/93‑1 (MQ=255)
                                                                        gTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGa                   <  1:1209943/93‑1 (MQ=255)
                                                                        gTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGa                   >  1:5248126/1‑93 (MQ=255)
                                                                         ttttCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGAt                  <  2:4134520/93‑1 (MQ=255)
                                                                         ttttCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGAt                  >  1:5267814/1‑93 (MQ=255)
                                                                           ttCTCGTGCCAGGCGATGCCGCGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTc                <  1:5485469/93‑1 (MQ=255)
                                                                             ctcGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTg              <  2:1668155/93‑1 (MQ=255)
                                                                                 tGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTc          <  2:516477/93‑1 (MQ=255)
                                                                                 gggcAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTc          <  2:6069983/90‑1 (MQ=255)
                                                                                  gCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCa         >  2:5859548/1‑93 (MQ=255)
                                                                                    cAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGa       <  1:1007156/93‑1 (MQ=255)
                                                                                    cAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGa       <  2:3750241/93‑1 (MQ=255)
                                                                                       gCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATaa    <  1:3291794/93‑1 (MQ=255)
                                                                                        cGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAg   <  2:2517814/93‑1 (MQ=255)
                                                                                        cGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAg   <  2:744042/93‑1 (MQ=255)
                                                                                         gATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAgc  <  1:4378396/93‑1 (MQ=255)
                                                                                           |                                                                                          
CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGC  >  NC_000913/3634679‑3634860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCC  >  NC_000913/3634677‑3634863
                                                                                             |                                                                                             
TGCCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGAC                                                                                               >  SRR4302547.496765/1‑94 (MQ=60)
TGCCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGAC                                                                                               >  SRR4302547.563750/1‑94 (MQ=60)
 GCCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACG                                                                                              <  SRR4302547.1924967/94‑1 (MQ=60)
  CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGC                                                                                             >  SRR4302547.88459/1‑94 (MQ=60)
  CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGC                                                                                             >  SRR4302547.3527377/1‑94 (MQ=60)
  CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGC                                                                                             >  SRR4302547.1751485/1‑94 (MQ=60)
  CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGC                                                                                             <  SRR4302547.4052888/94‑1 (MQ=60)
  CCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGC                                                                                             >  SRR4302547.5361764/1‑94 (MQ=60)
   CCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCC                                                                                            <  SRR4302547.3345821/94‑1 (MQ=60)
    CTTCTCGCGCTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCG                                                                                           <  SRR4302547.4402424/94‑1 (MQ=60)
    CTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCG                                                                                           >  SRR4302547.1842114/1‑94 (MQ=60)
    CTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCG                                                                                           >  SRR4302547.4387173/1‑94 (MQ=60)
     TTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGT                                                                                          <  SRR4302547.539427/94‑1 (MQ=60)
      TCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTG                                                                                         >  SRR4302547.3813534/1‑94 (MQ=60)
      TCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTG                                                                                         <  SRR4302547.2565777/94‑1 (MQ=60)
       CTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGT                                                                                        >  SRR4302547.6289107/1‑94 (MQ=60)
       CTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGT                                                                                        >  SRR4302547.4646200/1‑94 (MQ=60)
       CTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGT                                                                                        >  SRR4302547.3084626/1‑94 (MQ=60)
       CCCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGT                                                                                        <  SRR4302547.4359017/94‑1 (MQ=60)
        TCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTT                                                                                       <  SRR4302547.73654/94‑1 (MQ=60)
        TCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTGTTCTCGTGCCAGGCGATGCCGTGTT                                                                                       <  SRR4302547.2863817/94‑1 (MQ=60)
         CGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTT                                                                                      >  SRR4302547.2180121/1‑94 (MQ=60)
         CGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTT                                                                                      >  SRR4302547.2001286/1‑94 (MQ=60)
         CGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTT                                                                                      >  SRR4302547.5519577/1‑94 (MQ=60)
          GCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTA                                                                                     <  SRR4302547.1890064/94‑1 (MQ=60)
           CACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTAT                                                                                    >  SRR4302547.1209699/1‑94 (MQ=60)
            ACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATT                                                                                   >  SRR4302547.5238054/1‑94 (MQ=60)
            ACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATT                                                                                   >  SRR4302547.1586976/1‑94 (MQ=60)
             CTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTG                                                                                  <  SRR4302547.4808392/94‑1 (MQ=60)
             CTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTG                                                                                  >  SRR4302547.5487725/1‑94 (MQ=60)
             CTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTG                                                                                  <  SRR4302547.2639461/94‑1 (MQ=60)
              ccacCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGA                                                                                 <  SRR4302547.5172553/90‑1 (MQ=60)
                ATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACC                                                                               <  SRR4302547.3320260/94‑1 (MQ=60)
                 TCCGCCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCA                                                                              <  SRR4302547.1551066/94‑1 (MQ=60)
                 TCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCA                                                                              <  SRR4302547.863632/94‑1 (MQ=60)
                  CCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAG                                                                             <  SRR4302547.2745182/94‑1 (MQ=60)
                   CACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGA                                                                            <  SRR4302547.422096/94‑1 (MQ=60)
                    ACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGAT                                                                           <  SRR4302547.3424937/94‑1 (MQ=60)
                     CCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATC                                                                          <  SRR4302547.4420792/94‑1 (MQ=60)
                     CCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATC                                                                          <  SRR4302547.923932/94‑1 (MQ=60)
                     CCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATC                                                                          >  SRR4302547.5042004/1‑94 (MQ=60)
                      CAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCA                                                                         <  SRR4302547.2225754/94‑1 (MQ=60)
                      CAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCA                                                                         >  SRR4302547.2574304/1‑94 (MQ=60)
                      CAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCA                                                                         <  SRR4302547.1294411/94‑1 (MQ=60)
                       AGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAA                                                                        >  SRR4302547.2320341/1‑94 (MQ=60)
                        GCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAAT                                                                       >  SRR4302547.3458803/1‑94 (MQ=60)
                         CATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATG                                                                      >  SRR4302547.2083851/1‑94 (MQ=60)
                         CATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATG                                                                      <  SRR4302547.1201831/94‑1 (MQ=60)
                          ATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGA                                                                     >  SRR4302547.2135806/1‑94 (MQ=60)
                          ATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGA                                                                     <  SRR4302547.1272842/94‑1 (MQ=60)
                          ATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGA                                                                     <  SRR4302547.5546850/94‑1 (MQ=60)
                            GTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAA                                                                   >  SRR4302547.4909606/1‑94 (MQ=60)
                             TCGACAATATGCGGCGCGGAGTCATCGCAGAAGAGGTGCCCTAACGTATTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGATAT                                                                  <  SRR4302547.5466116/94‑1 (MQ=60)
                              CGACAATCTGCTGCGCGGCGACATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCCATGAGATC                                                                 >  SRR4302547.1017886/1‑94 (MQ=60)
                              CGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATC                                                                 <  SRR4302547.4313111/94‑1 (MQ=60)
                              CGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATC                                                                 <  SRR4302547.4333762/94‑1 (MQ=60)
                              CGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATC                                                                 >  SRR4302547.4214105/1‑94 (MQ=60)
                                ACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCC                                                               >  SRR4302547.268670/1‑94 (MQ=60)
                                ACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCC                                                               <  SRR4302547.1847724/94‑1 (MQ=60)
                                ACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCC                                                               <  SRR4302547.4653842/94‑1 (MQ=60)
                                  AATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCAC                                                             <  SRR4302547.4982194/94‑1 (MQ=60)
                                  AATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCAC                                                             <  SRR4302547.3854687/94‑1 (MQ=60)
                                  AATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCAC                                                             >  SRR4302547.633094/1‑94 (MQ=60)
                                   ATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACT                                                            >  SRR4302547.1480463/1‑94 (MQ=60)
                                   ATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACT                                                            <  SRR4302547.5710519/94‑1 (MQ=60)
                                   ATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACT                                                            >  SRR4302547.3677546/1‑94 (MQ=60)
                                    TCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTG                                                           <  SRR4302547.3325990/94‑1 (MQ=60)
                                    TCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTG                                                           <  SRR4302547.4115986/94‑1 (MQ=60)
                                    TCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTG                                                           >  SRR4302547.3651535/1‑94 (MQ=60)
                                       GCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGT                                                        <  SRR4302547.5791844/94‑1 (MQ=60)
                                        CTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTA                                                       >  SRR4302547.1119046/1‑94 (MQ=60)
                                        CTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTA                                                       >  SRR4302547.6094235/1‑94 (MQ=60)
                                         TGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAA                                                      >  SRR4302547.2771258/1‑94 (MQ=60)
                                          GCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAA                                                     >  SRR4302547.428086/1‑94 (MQ=60)
                                           CGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAA                                                    >  SRR4302547.851573/1‑94 (MQ=60)
                                            GCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAAC                                                   <  SRR4302547.4070421/94‑1 (MQ=60)
                                            GCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTTACCAGATCAATGAAATCCCACTGGGTAAAAC                                                   >  SRR4302547.792983/1‑94 (MQ=60)
                                             ggcAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGGCCAGATCAATGAAATCCCACTGGGTAAAACG                                                  <  SRR4302547.2664951/91‑1 (MQ=60)
                                             CGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACG                                                  >  SRR4302547.156516/1‑94 (MQ=60)
                                             CGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACG                                                  <  SRR4302547.5877688/94‑1 (MQ=60)
                                              TGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGT                                                 >  SRR4302547.5445417/1‑94 (MQ=60)
                                              GGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGT                                                 <  SRR4302547.4350985/94‑1 (MQ=60)
                                              GGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGT                                                 >  SRR4302547.4805751/1‑94 (MQ=60)
                                              GGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGT                                                 <  SRR4302547.1388079/94‑1 (MQ=60)
                                              GGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGT                                                 >  SRR4302547.4041004/1‑94 (MQ=60)
                                               GAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTG                                                >  SRR4302547.4424989/1‑94 (MQ=60)
                                               GAGTCAACACAGAAGACTTGCGCTAACGTTTTCTCGTGCCAGGGGACGCCGTGTTTATTGACCAGATCAATGAAATCTCGCTGGGTAAAACGTG                                                <  SRR4302547.5000914/94‑1 (MQ=60)
                                                AGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGC                                               <  SRR4302547.4327114/94‑1 (MQ=60)
                                                AGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGC                                               <  SRR4302547.6114189/94‑1 (MQ=60)
                                                AGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGC                                               <  SRR4302547.2160106/94‑1 (MQ=60)
                                                 GTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCG                                              >  SRR4302547.1211373/1‑94 (MQ=60)
                                                  TCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGA                                             <  SRR4302547.87577/94‑1 (MQ=60)
                                                   CATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCACTGAAATCCCACTGGGTAAAACGTGCGAG                                            <  SRR4302547.2509492/94‑1 (MQ=60)
                                                   CATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAG                                            >  SRR4302547.6130111/1‑94 (MQ=60)
                                                    ATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGT                                           <  SRR4302547.5915302/94‑1 (MQ=60)
                                                    ATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGT                                           <  SRR4302547.702060/94‑1 (MQ=60)
                                                     TCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTG                                          <  SRR4302547.170353/94‑1 (MQ=60)
                                                     TCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTG                                          <  SRR4302547.6768775/94‑1 (MQ=60)
                                                     TCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTG                                          <  SRR4302547.177844/94‑1 (MQ=60)
                                                     TCGCAGAAGAGTTGCCCTAACGTTTCCTCGTGCCAGGCGACGCCGTGTTTATTGCCCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTG                                          <  SRR4302547.1791993/94‑1 (MQ=60)
                                                        CAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAG                                       <  SRR4302547.4272493/94‑1 (MQ=60)
                                                        CAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAG                                       <  SRR4302547.5939426/94‑1 (MQ=60)
                                                        CAGAAGAGTGGCCCTAACGTTTTCTCGTGCCAGGCGACTCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAG                                       <  SRR4302547.521485/94‑1 (MQ=60)
                                                         AGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGA                                      <  SRR4302547.2519150/94‑1 (MQ=60)
                                                           AAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACT                                    <  SRR4302547.6017797/94‑1 (MQ=60)
                                                            AGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTT                                   <  SRR4302547.3818319/94‑1 (MQ=60)
                                                                TTGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAA                               <  SRR4302547.3275702/94‑1 (MQ=60)
                                                                 TGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAA                              >  SRR4302547.2135483/1‑94 (MQ=60)
                                                                 TGCCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAA                              <  SRR4302547.2814962/94‑1 (MQ=60)
                                                                  GGCCTAACGGTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCTCACTCGGTAAAACGTGCGAGTGCAGACTTACAAGA                             >  SRR4302547.3223300/1‑94 (MQ=60)
                                                                   CCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTTAAACGTGCGAGTGCAGACTTACAAAAA                            >  SRR4302547.6196664/1‑94 (MQ=60)
                                                                   CCCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAA                            >  SRR4302547.5095330/1‑94 (MQ=60)
                                                                    CCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAAT                           >  SRR4302547.3848005/1‑94 (MQ=60)
                                                                    CCTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAAT                           >  SRR4302547.4238447/1‑94 (MQ=60)
                                                                     CTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATG                          >  SRR4302547.4405327/1‑94 (MQ=60)
                                                                     CTAACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATG                          <  SRR4302547.5220271/94‑1 (MQ=60)
                                                                      TAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGC                         >  SRR4302547.2502367/1‑94 (MQ=60)
                                                                       AACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCG                        >  SRR4302547.6557163/1‑94 (MQ=60)
                                                                       AACGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCG                        <  SRR4302547.1250848/94‑1 (MQ=60)
                                                                        ACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGG                       <  SRR4302547.2382603/94‑1 (MQ=60)
                                                                        ACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGG                       <  SRR4302547.3211606/94‑1 (MQ=60)
                                                                         CGTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGA                      >  SRR4302547.5356165/1‑94 (MQ=60)
                                                                          GTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGAT                     >  SRR4302547.5376333/1‑94 (MQ=60)
                                                                          GTTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGAT                     <  SRR4302547.1234360/94‑1 (MQ=60)
                                                                           TTTTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATT                    <  SRR4302547.2843392/94‑1 (MQ=60)
                                                                             TTCTCGTGCCAGGCGATGCCGCGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCT                  <  SRR4302547.5598743/94‑1 (MQ=60)
                                                                             TTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCT                  <  SRR4302547.5488650/94‑1 (MQ=60)
                                                                             TTCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCT                  >  SRR4302547.4704813/1‑94 (MQ=60)
                                                                             ATCTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCT                  >  SRR4302547.3911601/1‑94 (MQ=60)
                                                                               CTCGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGG                >  SRR4302547.4941082/1‑94 (MQ=60)
                                                                                 CGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCT              <  SRR4302547.4800991/94‑1 (MQ=60)
                                                                                 CGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCT              <  SRR4302547.1064342/94‑1 (MQ=60)
                                                                                 CGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCT              <  SRR4302547.1230526/94‑1 (MQ=60)
                                                                                 CGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCT              <  SRR4302547.2344044/94‑1 (MQ=60)
                                                                                 CGTGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCT              >  SRR4302547.6498574/1‑94 (MQ=60)
                                                                                   TGCCAGGCTACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCA            <  SRR4302547.5678678/94‑1 (MQ=60)
                                                                                   TGCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCA            <  SRR4302547.507406/94‑1 (MQ=60)
                                                                                    GCCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAG           >  SRR4302547.1352674/1‑94 (MQ=60)
                                                                                     CCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGA          >  SRR4302547.819618/1‑94 (MQ=60)
                                                                                     CCAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGA          <  SRR4302547.1219053/94‑1 (MQ=60)
                                                                                      CAGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGAT         <  SRR4302547.1026820/94‑1 (MQ=60)
                                                                                       AGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATA        >  SRR4302547.6340319/1‑94 (MQ=60)
                                                                                       AGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATA        <  SRR4302547.1763936/94‑1 (MQ=60)
                                                                                       AGGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATA        >  SRR4302547.5148463/1‑94 (MQ=60)
                                                                                        GGCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAA       <  SRR4302547.4887296/94‑1 (MQ=60)
                                                                                         GCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAG      <  SRR4302547.3357897/94‑1 (MQ=60)
                                                                                         GCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAG      <  SRR4302547.4067196/94‑1 (MQ=60)
                                                                                         GCGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAG      <  SRR4302547.3063995/94‑1 (MQ=60)
                                                                                          CGACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGC     >  SRR4302547.1980969/1‑94 (MQ=60)
                                                                                           GATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCG    <  SRR4302547.4468394/94‑1 (MQ=60)
                                                                                           GACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCG    >  SRR4302547.6443920/1‑94 (MQ=60)
                                                                                           GACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCG    <  SRR4302547.3933168/94‑1 (MQ=60)
                                                                                           GACGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCG    >  SRR4302547.5690483/1‑94 (MQ=60)
                                                                                             CGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCC  >  SRR4302547.6370021/1‑94 (MQ=60)
                                                                                             |                                                                                             
TGCCCTTCTCGCACTCATCCACCAGCATGTCGACAATCTGCTGCGCGGAGTCATCGCAGAAGAGTTGCCCTAACGTTTTCTCGTGCCAGGCGATGCCGTGTTTATTGACCAGATCAATGAAATCCCACTGGGTAAAACGTGCGAGTGCAGACTTACAAAAATGCGGATTCTGGCTCAGATAAGCGCC  >  NC_000913/3634677‑3634863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑