Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,659,028 A→G T329A (ACC→GCC)  yhiV → putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,659,0280AG80.4% 618.5 / 154.6 287T329A (ACC→GCC) yhiVputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base A (31/25);  new base G (121/109);  total (152/135)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 302 total aligned reads displayed.

GCCCTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  NC_000913/3658938‑3659120
                                                                                          |                                                                                            
   cTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACg                                                                                         >  2:2206459/1‑93 (MQ=255)
    tGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGc                                                                                        <  2:3308127/93‑1 (MQ=255)
    tGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGc                                                                                        >  2:6393553/1‑93 (MQ=255)
    tGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGc                                                                                        <  1:2410789/93‑1 (MQ=255)
    tGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGc                                                                                        >  2:2802139/1‑93 (MQ=255)
      gATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCg                                                                                      <  2:361082/93‑1 (MQ=255)
        tACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGtt                                                                                    <  2:6327357/93‑1 (MQ=255)
           cTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTAt                                                                                 <  2:1353498/93‑1 (MQ=255)
           cTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTAt                                                                                 <  2:6112171/93‑1 (MQ=255)
           cTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTAt                                                                                 <  2:4611980/93‑1 (MQ=255)
             cgcgGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCg                                                                               >  2:1127525/1‑93 (MQ=255)
             cgcgGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCg                                                                               <  2:4218343/93‑1 (MQ=255)
              gcgGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGa                                                                              >  2:2173896/1‑93 (MQ=255)
               cgGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGaa                                                                             >  2:2273548/1‑93 (MQ=255)
               cgGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGaa                                                                             >  1:2960717/1‑93 (MQ=255)
               cgGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGGTTATCCTTACGACACCACGCCGTTTATCGaa                                                                             >  1:1126393/1‑93 (MQ=255)
                gggCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGaaa                                                                            <  2:3698434/93‑1 (MQ=255)
                gggCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGaaa                                                                            >  1:465893/1‑93 (MQ=255)
                gggCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGaaa                                                                            >  1:4001374/1‑93 (MQ=255)
                 ggCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAAt                                                                           <  1:4784075/93‑1 (MQ=255)
                 ggCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAAt                                                                           >  2:3877460/1‑93 (MQ=255)
                  gCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAATACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAAtt                                                                          >  2:627389/1‑93 (MQ=255)
                  gCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAAtt                                                                          >  2:3244496/1‑93 (MQ=255)
                  gCAGTAAAAGAGAAACTCAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAAtt                                                                          <  2:5870130/93‑1 (MQ=255)
                    aGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTc                                                                        >  1:5331167/1‑93 (MQ=255)
                     gTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCt                                                                       >  1:4983193/1‑93 (MQ=255)
                     atCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCt                                                                       >  1:6384074/2‑93 (MQ=255)
                       cAAAGAGGAGCTGATCCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTAt                                                                     <  1:3755129/93‑1 (MQ=255)
                          agagGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCa                                                                  <  1:3696718/93‑1 (MQ=255)
                          agagGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCa                                                                  >  1:5469163/1‑93 (MQ=255)
                          agagGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCa                                                                  >  1:1896659/1‑93 (MQ=255)
                            agGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAgg                                                                <  2:2830126/93‑1 (MQ=255)
                               aaCTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAg                                                             >  1:4136067/1‑93 (MQ=255)
                                aCTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGt                                                            >  1:1768093/1‑93 (MQ=255)
                                 cTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGtt                                                           <  2:1915135/93‑1 (MQ=255)
                                  tGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGttt                                                          <  2:2422250/93‑1 (MQ=255)
                                    aaCCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTc                                                        <  1:4852051/93‑1 (MQ=255)
                                    aaCCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTc                                                        >  2:1434727/1‑93 (MQ=255)
                                      ccGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCaa                                                      <  1:5974169/93‑1 (MQ=255)
                                        gCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCaaaa                                                    <  2:5584645/93‑1 (MQ=255)
                                        gCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGGTTATCCTTACGACGCCACGCCGCTTATCGAAATTTCTATTCAGGAAGTTTTCaaaa                                                    >  1:752353/1‑93 (MQ=255)
                                         cTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAac                                                   >  2:3457870/1‑93 (MQ=255)
                                           tATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAacac                                                 >  2:4379556/1‑93 (MQ=255)
                                             tCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTg                                               <  1:728688/93‑1 (MQ=255)
                                             tCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTg                                               >  2:508490/1‑93 (MQ=255)
                                              cAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGCAATTTCTATTCAGGAAGTTTTCAAAACACTgg                                              >  1:4907604/1‑93 (MQ=255)
                                              cAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTgg                                              >  1:1980651/1‑93 (MQ=255)
                                                gCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGtt                                            <  1:2566779/93‑1 (MQ=255)
                                                gCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGtt                                            >  2:1024300/1‑93 (MQ=255)
                                                gCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGtt                                            <  1:2149329/93‑1 (MQ=255)
                                                gCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGtt                                            <  2:4857611/93‑1 (MQ=255)
                                                gCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGtt                                            >  1:3308119/1‑93 (MQ=255)
                                                 ccTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTg                                           >  1:427464/1‑93 (MQ=255)
                                                   tATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAGAACACTGGTTGAg                                         <  1:5819490/93‑1 (MQ=255)
                                                   tATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAg                                         >  1:3461502/1‑93 (MQ=255)
                                                   tATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAg                                         <  2:810929/93‑1 (MQ=255)
                                                   tATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAg                                         <  2:4080844/93‑1 (MQ=255)
                                                    aTTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAgg                                        <  1:2890095/93‑1 (MQ=255)
                                                     tttCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGc                                       <  1:1044967/93‑1 (MQ=255)
                                                     tttCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGc                                       <  1:3120542/93‑1 (MQ=255)
                                                     tttCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGc                                       <  1:2485036/93‑1 (MQ=255)
                                                     tttCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGc                                       >  1:6561675/1‑93 (MQ=255)
                                                      ttCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCAAGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCt                                      >  2:3187599/1‑93 (MQ=255)
                                                        cccGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGCTTATCGAAATTTCTATTCAGGAAGTTTTCAACACACTGGTTGAGGCTat                                    >  2:4710619/1‑93 (MQ=255)
                                                         ccGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTatc                                   >  2:596157/1‑93 (MQ=255)
                                                          cGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTatca                                  <  2:6639917/93‑1 (MQ=255)
                                                           ggCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTatcat                                 >  1:5592916/1‑93 (MQ=255)
                                                           ggCAAGTCTGAAGACGGATTATGCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGATatcat                                 >  2:6498689/1‑93 (MQ=255)
                                                            gCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTatcatc                                <  1:6062122/93‑1 (MQ=255)
                                                             cAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATcc                               <  2:1590768/93‑1 (MQ=255)
                                                              aaGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCt                              >  2:1293674/1‑93 (MQ=255)
                                                              aaGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCt                              >  1:444534/1‑93 (MQ=255)
                                                               aGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTa                             >  1:5209112/1‑93 (MQ=255)
                                                                gTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAg                            >  2:5239765/1‑93 (MQ=255)
                                                                gTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAg                            >  2:3388187/1‑93 (MQ=255)
                                                                  cTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTc                          <  1:5092489/93‑1 (MQ=255)
                                                                   tGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCt                         <  1:267778/93‑1 (MQ=255)
                                                                   tGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCt                         >  2:1812366/1‑93 (MQ=255)
                                                                      aGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTcc                      >  1:908507/1‑93 (MQ=255)
                                                                       gACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCt                     <  1:3578960/93‑1 (MQ=255)
                                                                       gACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCt                     <  2:1652781/93‑1 (MQ=255)
                                                                        aCGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTg                    <  1:574097/93‑1 (MQ=255)
                                                                        aCGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTg                    <  1:1306525/93‑1 (MQ=255)
                                                                           gTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTc                 <  2:6220096/93‑1 (MQ=255)
                                                                            tttATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCa                >  2:2692240/1‑93 (MQ=255)
                                                                             ttATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCAt               <  2:5642990/93‑1 (MQ=255)
                                                                             ttATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCAt               >  2:4812150/1‑93 (MQ=255)
                                                                              tATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATg              <  2:3125931/93‑1 (MQ=255)
                                                                               aTCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGt             >  1:1003583/1‑93 (MQ=255)
                                                                                 ccTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTAt           >  2:4332828/1‑93 (MQ=255)
                                                                                  cTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATc          <  1:3187862/93‑1 (MQ=255)
                                                                                   ttACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCt         <  1:1637472/93‑1 (MQ=255)
                                                                                    tACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTg        >  2:5711034/1‑93 (MQ=255)
                                                                                    tACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTg        >  1:394881/1‑93 (MQ=255)
                                                                                    tACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTg        <  2:304764/93‑1 (MQ=255)
                                                                                     acgacgCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGt       >  2:2540927/1‑93 (MQ=255)
                                                                                     aCGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGt       <  1:1376449/93‑1 (MQ=255)
                                                                                      cGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGtt      <  2:717127/93‑1 (MQ=255)
                                                                                       gACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGttt     >  2:5288247/1‑93 (MQ=255)
                                                                                          accacGCCATTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTg  >  1:6480251/1‑93 (MQ=255)
                                                                                          |                                                                                            
GCCCTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  NC_000913/3658938‑3659120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  NC_000913/3658937‑3659120
                                                                                           |                                                                                            
CGCCCTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCC                                                                                            >  SRR4302547.331313/1‑94 (MQ=60)
 GCCCTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCA                                                                                           >  SRR4302547.611484/1‑94 (MQ=60)
    CTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGC                                                                                        >  SRR4302547.3782070/1‑94 (MQ=60)
    CTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGC                                                                                        >  SRR4302547.745469/1‑94 (MQ=60)
    CTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGC                                                                                        >  SRR4302547.5068492/1‑94 (MQ=60)
    CTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGC                                                                                        >  SRR4302547.834239/1‑94 (MQ=60)
    CTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAAACGCTTATCAGCCTATTTCCCGGCAAGTCTGATGACGGTTTATCCTTACGACGCCCCGC                                                                                        >  SRR4302547.4658548/1‑94 (MQ=60)
     TGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCC                                                                                       <  SRR4302547.4602694/94‑1 (MQ=60)
     TGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCC                                                                                       <  SRR4302547.2459594/94‑1 (MQ=60)
      GGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCG                                                                                      <  SRR4302547.1041636/94‑1 (MQ=60)
      GGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCG                                                                                      >  SRR4302547.5116527/1‑94 (MQ=60)
       GATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGT                                                                                     >  SRR4302547.5125229/1‑94 (MQ=60)
          GCCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCCCGCCGTTTA                                                                                  <  SRR4302547.3849653/94‑1 (MQ=60)
          ACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTA                                                                                  <  SRR4302547.2394562/94‑1 (MQ=60)
            CTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATC                                                                                >  SRR4302547.2074105/1‑94 (MQ=60)
              CGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGA                                                                              >  SRR4302547.3202844/1‑94 (MQ=60)
               GCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAA                                                                             >  SRR4302547.3019649/1‑94 (MQ=60)
               GCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGGTTATCCTTACGACACCACGCCGTTTATCGAA                                                                             >  SRR4302547.1149124/1‑94 (MQ=60)
                CGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAA                                                                            >  SRR4302547.474478/1‑94 (MQ=60)
                CGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAA                                                                            >  SRR4302547.4082706/1‑94 (MQ=60)
                CGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAA                                                                            >  SRR4302547.4930488/1‑94 (MQ=60)
                CGGGCAGTCAAAGAGGAACTGAACAGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAA                                                                            >  SRR4302547.5446958/1‑94 (MQ=60)
                  GGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATT                                                                          >  SRR4302547.757915/1‑94 (MQ=60)
                  GGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATT                                                                          <  SRR4302547.4883158/94‑1 (MQ=60)
                   GCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTT                                                                         <  SRR4302547.2008274/94‑1 (MQ=60)
                   GCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTT                                                                         >  SRR4302547.501154/1‑94 (MQ=60)
                    CAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTC                                                                        >  SRR4302547.5441049/1‑94 (MQ=60)
                     AGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGCCACCACGCCGTTTATCGAAATTTCT                                                                       >  SRR4302547.1915379/1‑94 (MQ=60)
                     AGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCT                                                                       <  SRR4302547.6119824/94‑1 (MQ=60)
                     AGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCT                                                                       >  SRR4302547.5086248/1‑94 (MQ=60)
                     AATCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCT                                                                       >  SRR4302547.6517295/1‑94 (MQ=60)
                      GTCAAAGAGGAACTTAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTA                                                                      <  SRR4302547.1510026/94‑1 (MQ=60)
                       TCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTAT                                                                     <  SRR4302547.1050310/94‑1 (MQ=60)
                        CAAAGAGGAGCTGATCCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATT                                                                    <  SRR4302547.3831751/94‑1 (MQ=60)
                        CAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATT                                                                    <  SRR4302547.6486222/94‑1 (MQ=60)
                        CAAAGAGGAACTCAACCGCTTATCACCGTATTTCCCGGCACGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATG                                                                    <  SRR4302547.5264278/94‑1 (MQ=60)
                          AAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACTCCACGCCGTTTATCGAAATTTCTATTCA                                                                  <  SRR4302547.923721/94‑1 (MQ=60)
                          AAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCA                                                                  >  SRR4302547.5582153/1‑94 (MQ=60)
                          AAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCA                                                                  >  SRR4302547.1934168/1‑94 (MQ=60)
                           AGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAG                                                                 <  SRR4302547.3772174/94‑1 (MQ=60)
                           AGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAG                                                                 >  SRR4302547.1523089/1‑94 (MQ=60)
                           AGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAG                                                                 >  SRR4302547.10921/1‑94 (MQ=60)
                           AGAGAAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAG                                                                 <  SRR4302547.5717769/94‑1 (MQ=60)
                             AGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGA                                                               <  SRR4302547.4494083/94‑1 (MQ=60)
                               GAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAG                                                             >  SRR4302547.4220222/1‑94 (MQ=60)
                                AACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGT                                                            >  SRR4302547.1803154/1‑94 (MQ=60)
                                  agcccctttttttatttttttgccgcgcaagttaaaaagcggtTTTATCTTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTT                                                          <  SRR4302547.3349373/51‑1 (MQ=60)
                                  CTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTT                                                          >  SRR4302547.5803288/1‑94 (MQ=60)
                                  CTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTT                                                          <  SRR4302547.2538307/94‑1 (MQ=60)
                                  CTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTT                                                          <  SRR4302547.494179/94‑1 (MQ=60)
                                  CTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTT                                                          >  SRR4302547.1954093/1‑94 (MQ=60)
                                   TGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTT                                                         <  SRR4302547.5503518/94‑1 (MQ=60)
                                     AACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCA                                                       <  SRR4302547.4952591/94‑1 (MQ=60)
                                     AACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCA                                                       >  SRR4302547.2507218/1‑94 (MQ=60)
                                      ACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAA                                                      <  SRR4302547.432168/94‑1 (MQ=60)
                                      ACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAA                                                      >  SRR4302547.5320424/1‑94 (MQ=60)
                                       CCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAA                                                     <  SRR4302547.6098081/94‑1 (MQ=60)
                                        CGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGGTTATCCTTACGACGCCACGCCGCTTATCGAAATTTCTATTCAGGAAGTTTTCAAAA                                                    >  SRR4302547.766541/1‑94 (MQ=60)
                                           TTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACAC                                                 >  SRR4302547.4805214/1‑94 (MQ=60)
                                            TATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACT                                                <  SRR4302547.5648093/94‑1 (MQ=60)
                                             ATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTG                                               >  SRR4302547.3386665/1‑94 (MQ=60)
                                             ATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTAAAAAAACTG                                               >  SRR4302547.4359861/1‑94 (MQ=60)
                                              TCAGCTTATTTCCTGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGG                                              <  SRR4302547.3191180/94‑1 (MQ=60)
                                              TCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGCAATTTCTATTCAGGAAGTTTTCAAAACACTGG                                              >  SRR4302547.5009236/1‑94 (MQ=60)
                                              TCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGG                                              <  SRR4302547.4424956/94‑1 (MQ=60)
                                              TCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGG                                              >  SRR4302547.2019980/1‑94 (MQ=60)
                                              TCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGG                                              <  SRR4302547.742282/94‑1 (MQ=60)
                                              TCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGG                                              <  SRR4302547.3507336/94‑1 (MQ=60)
                                               CAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGT                                             >  SRR4302547.3751296/1‑94 (MQ=60)
                                                AGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTT                                            >  SRR4302547.3744288/1‑94 (MQ=60)
                                                AGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTT                                            >  SRR4302547.2517029/1‑94 (MQ=60)
                                                AGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTT                                            <  SRR4302547.1264772/94‑1 (MQ=60)
                                                AGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTT                                            >  SRR4302547.3374822/1‑94 (MQ=60)
                                                 GCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTG                                           <  SRR4302547.2192225/94‑1 (MQ=60)
                                                 GCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTG                                           <  SRR4302547.2618622/94‑1 (MQ=60)
                                                 GCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTG                                           >  SRR4302547.435380/1‑94 (MQ=60)
                                                 GCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTG                                           <  SRR4302547.6620398/94‑1 (MQ=60)
                                                   CTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAG                                         >  SRR4302547.6721705/1‑94 (MQ=60)
                                                   CTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAG                                         >  SRR4302547.3532090/1‑94 (MQ=60)
                                                   CTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAG                                         <  SRR4302547.1894302/94‑1 (MQ=60)
                                                   CTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAG                                         <  SRR4302547.4000102/94‑1 (MQ=60)
                                                    TATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAGAACACTGGTTGAGG                                        <  SRR4302547.5939890/94‑1 (MQ=60)
                                                    TATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGG                                        >  SRR4302547.936528/1‑94 (MQ=60)
                                                    TATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGG                                        >  SRR4302547.1379402/1‑94 (MQ=60)
                                                     ATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGC                                       <  SRR4302547.2947740/94‑1 (MQ=60)
                                                     ATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGC                                       >  SRR4302547.6698310/1‑94 (MQ=60)
                                                      TTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCT                                      <  SRR4302547.1065607/94‑1 (MQ=60)
                                                      TTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCT                                      >  SRR4302547.602448/1‑94 (MQ=60)
                                                      TTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCT                                      <  SRR4302547.3182414/94‑1 (MQ=60)
                                                      TTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCT                                      <  SRR4302547.2535247/94‑1 (MQ=60)
                                                       TTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTA                                     <  SRR4302547.6355722/94‑1 (MQ=60)
                                                        TCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTAT                                    <  SRR4302547.2919569/94‑1 (MQ=60)
                                                          CCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCA                                  <  SRR4302547.1281753/94‑1 (MQ=60)
                                                          CCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCA                                  >  SRR4302547.3558328/1‑94 (MQ=60)
                                                          CCGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCA                                  <  SRR4302547.2541741/94‑1 (MQ=60)
                                                          CCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCA                                  >  SRR4302547.5933264/1‑94 (MQ=60)
                                                           TGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGGAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCAT                                 >  SRR4302547.1782784/1‑94 (MQ=60)
                                                           CGGCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCAT                                 >  SRR4302547.5708294/1‑94 (MQ=60)
                                                           CGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCAT                                 >  SRR4302547.1120784/1‑94 (MQ=60)
                                                             GCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCC                               <  SRR4302547.4949580/94‑1 (MQ=60)
                                                             GCAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCC                               <  SRR4302547.6187866/94‑1 (MQ=60)
                                                              CAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCT                              <  SRR4302547.3181364/94‑1 (MQ=60)
                                                              CAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCT                              <  SRR4302547.3092271/94‑1 (MQ=60)
                                                              CAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCT                              <  SRR4302547.1749857/94‑1 (MQ=60)
                                                              CAAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCT                              >  SRR4302547.2990189/1‑94 (MQ=60)
                                                              CAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCT                              >  SRR4302547.452813/1‑94 (MQ=60)
                                                               AAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTA                             >  SRR4302547.416854/1‑94 (MQ=60)
                                                               AAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTA                             >  SRR4302547.4065677/1‑94 (MQ=60)
                                                               AAGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTA                             >  SRR4302547.5316313/1‑94 (MQ=60)
                                                                AGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAG                            >  SRR4302547.2620832/1‑94 (MQ=60)
                                                                AGTCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAG                            <  SRR4302547.6682508/94‑1 (MQ=60)
                                                                  TCTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTC                          <  SRR4302547.3599865/94‑1 (MQ=60)
                                                                   CTGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCT                         <  SRR4302547.5197519/94‑1 (MQ=60)
                                                                    TGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTT                        <  SRR4302547.2046920/94‑1 (MQ=60)
                                                                    TGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTT                        <  SRR4302547.272789/94‑1 (MQ=60)
                                                                    TGAAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTT                        <  SRR4302547.585894/94‑1 (MQ=60)
                                                                      AAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCC                      >  SRR4302547.926015/1‑94 (MQ=60)
                                                                      AAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCC                      <  SRR4302547.2997757/94‑1 (MQ=60)
                                                                      AAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCC                      >  SRR4302547.6527719/1‑94 (MQ=60)
                                                                      AAGACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCC                      <  SRR4302547.3938674/94‑1 (MQ=60)
                                                                        GACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTG                    <  SRR4302547.3651962/94‑1 (MQ=60)
                                                                         ACGGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGG                   <  SRR4302547.584404/94‑1 (MQ=60)
                                                                         ACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGG                   <  SRR4302547.1332799/94‑1 (MQ=60)
                                                                           GGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTC                 <  SRR4302547.65077/94‑1 (MQ=60)
                                                                           GGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTC                 >  SRR4302547.961754/1‑94 (MQ=60)
                                                                           GGTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTC                 >  SRR4302547.1374670/1‑94 (MQ=60)
                                                                            GTTTATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCA                <  SRR4302547.3777524/94‑1 (MQ=60)
                                                                             TTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCAT               >  SRR4302547.4881224/1‑94 (MQ=60)
                                                                             TTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCAT               >  SRR4302547.3267283/1‑94 (MQ=60)
                                                                               TATCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGT             >  SRR4302547.1023161/1‑94 (MQ=60)
                                                                                 TCCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTAT           >  SRR4302547.6065415/1‑94 (MQ=60)
                                                                                 TCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTAT           <  SRR4302547.2858236/94‑1 (MQ=60)
                                                                                  CCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATC          >  SRR4302547.5628715/1‑94 (MQ=60)
                                                                                  CCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATC          >  SRR4302547.2706572/1‑94 (MQ=60)
                                                                                  CCTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATC          >  SRR4302547.4039065/1‑94 (MQ=60)
                                                                                   CTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCT         >  SRR4302547.717791/1‑94 (MQ=60)
                                                                                   CTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCT         <  SRR4302547.3251385/94‑1 (MQ=60)
                                                                                   CTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCT         >  SRR4302547.2251674/1‑94 (MQ=60)
                                                                                   CTTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCT         >  SRR4302547.5259339/1‑94 (MQ=60)
                                                                                    TTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTG        >  SRR4302547.402215/1‑94 (MQ=60)
                                                                                    TTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTG        <  SRR4302547.1669843/94‑1 (MQ=60)
                                                                                    TTACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTG        <  SRR4302547.535052/94‑1 (MQ=60)
                                                                                      ACGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTT      <  SRR4302547.4968075/94‑1 (MQ=60)
                                                                                      ACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTT      <  SRR4302547.1404042/94‑1 (MQ=60)
                                                                                       CGACGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTT     >  SRR4302547.4212909/1‑94 (MQ=60)
                                                                                          CGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  SRR4302547.3283610/1‑94 (MQ=60)
                                                                                          CGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  SRR4302547.4546686/1‑94 (MQ=60)
                                                                                          CGCCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  SRR4302547.2124737/1‑94 (MQ=60)
                                                                                          CACCACGCCATTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  SRR4302547.6615166/1‑94 (MQ=60)
                                                                                           |                                                                                            
CGCCCTGGATACCTCGCGGGCAGTCAAAGAGGAACTGAACCGCTTATCAGCCTATTTCCCGGCAAGTCTGAAGACGGTTTATCCTTACGACACCACGCCGTTTATCGAAATTTCTATTCAGGAAGTTTTCAAAACACTGGTTGAGGCTATCATCCTAGTCTTCCTGGTCATGTATCTGTTTTTG  >  NC_000913/3658937‑3659120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑