Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,703,602 2 bp→GC intergenic (‑183/+124) dppB ← / ← dppA dipeptide transport system permease protein 1/dipeptide transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,703,6020CG100.0% 1007.0 / NA 283intergenic (‑183/+125)dppB/dppAdipeptide transport system permease protein 1/dipeptide transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (127/156);  total (127/156)
*NC_0009133,703,6030GC100.0% 1011.1 / NA 284intergenic (‑184/+124)dppB/dppAdipeptide transport system permease protein 1/dipeptide transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (126/158);  total (126/158)

Only 100 of 291 total aligned reads displayed.

TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGCGGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGC  >  NC_000913/3703515‑3703689
                                                                                       ||                                                                                      
tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTa                                                                                    >  1:3397601/1‑93 (MQ=255)
 gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                   <  1:5558131/93‑1 (MQ=255)
   tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACg                                                                                 >  1:1408495/1‑93 (MQ=255)
    gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGc                                                                                <  1:1571523/93‑1 (MQ=255)
     ccGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                               <  2:4286864/93‑1 (MQ=255)
      cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                              >  2:268717/1‑93 (MQ=255)
       ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                             <  1:970692/93‑1 (MQ=255)
        gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                            >  1:2713590/1‑93 (MQ=255)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                          >  2:3902674/1‑93 (MQ=255)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                          >  2:639273/1‑93 (MQ=255)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                          <  2:4214186/93‑1 (MQ=255)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                          <  2:4447436/93‑1 (MQ=255)
          tGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                          <  1:5153565/93‑1 (MQ=255)
           ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                         >  2:3011377/1‑93 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                      >  2:4672084/1‑93 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                      >  2:305401/1‑93 (MQ=255)
              gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                      >  1:1183052/1‑93 (MQ=255)
                 gCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTa                                                                   <  1:2038957/93‑1 (MQ=255)
                  cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                  <  2:2415095/93‑1 (MQ=255)
                   aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACa                                                                 >  2:5545768/1‑93 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                <  2:1662019/93‑1 (MQ=255)
                    cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                >  1:3811526/1‑93 (MQ=255)
                       agagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAt                                                             <  2:5160929/93‑1 (MQ=255)
                        gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                            >  1:3632041/1‑93 (MQ=255)
                        gagGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATc                                                            >  1:3831818/1‑93 (MQ=255)
                          ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                          >  2:5849178/1‑93 (MQ=255)
                             acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                       >  2:5428344/1‑93 (MQ=255)
                             acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                       >  1:5071500/1‑93 (MQ=255)
                             acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                       <  1:4878106/93‑1 (MQ=255)
                             acaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                       >  2:5193940/1‑93 (MQ=255)
                              caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCaa                                                      <  2:538281/93‑1 (MQ=255)
                                gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                    >  1:3732713/1‑93 (MQ=255)
                                   ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                 >  1:5977819/1‑93 (MQ=255)
                                    cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                <  1:527176/93‑1 (MQ=255)
                                    cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                <  1:2133325/93‑1 (MQ=255)
                                    cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa                                                >  2:1459651/1‑93 (MQ=255)
                                     aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                               <  1:6111202/93‑1 (MQ=255)
                                       cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                             <  1:2787473/93‑1 (MQ=255)
                                         ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                           >  1:2521072/1‑93 (MQ=255)
                                         ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                           <  2:2832656/93‑1 (MQ=255)
                                         ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                           <  1:3792612/93‑1 (MQ=255)
                                          gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                          <  2:5276657/93‑1 (MQ=255)
                                          gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                          <  2:2252112/93‑1 (MQ=255)
                                           tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                         <  2:4880821/93‑1 (MQ=255)
                                           tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                         <  2:1358784/93‑1 (MQ=255)
                                            ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                        >  2:503220/1‑93 (MQ=255)
                                            ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                        <  1:3528443/93‑1 (MQ=255)
                                            ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                        <  1:3196962/93‑1 (MQ=255)
                                             taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                       >  2:4499898/1‑93 (MQ=255)
                                             taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                       >  1:2935981/1‑93 (MQ=255)
                                              aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                      >  2:5613583/1‑93 (MQ=255)
                                              aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                      >  2:2644946/1‑93 (MQ=255)
                                              aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                      >  2:181417/1‑93 (MQ=255)
                                              aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                      <  2:1512662/93‑1 (MQ=255)
                                               cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                     <  1:385487/93‑1 (MQ=255)
                                                  aTTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt                                  <  1:6121197/93‑1 (MQ=255)
                                                   ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAGATCGTGCAAATTCAATACATTGCAGAAttt                                 >  1:6009948/1‑93 (MQ=255)
                                                    tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtttt                                >  1:195777/1‑93 (MQ=255)
                                                      ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCg                              >  2:3340061/1‑93 (MQ=255)
                                                      ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCg                              >  1:913692/1‑93 (MQ=255)
                                                      ttgACAAAGAGCGCTTTGTTTATGCCGGACGCGGCGTTAACACCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCg                              >  2:4125714/1‑93 (MQ=14)
                                                       tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGt                             >  1:1346953/1‑93 (MQ=255)
                                                        gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                            <  2:364163/93‑1 (MQ=255)
                                                        gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                            >  2:1907904/1‑93 (MQ=255)
                                                        gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTa                            >  2:2907838/1‑93 (MQ=255)
                                                         aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAg                           <  2:1219874/93‑1 (MQ=255)
                                                         aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAg                           <  1:1671857/93‑1 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAgg                          >  2:3210843/1‑93 (MQ=255)
                                                          cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAgg                          <  2:883515/93‑1 (MQ=255)
                                                           aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGc                         >  2:4736936/1‑93 (MQ=255)
                                                            aaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGcc                        >  2:3565529/1‑93 (MQ=255)
                                                              gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTg                      >  1:391575/1‑93 (MQ=255)
                                                               tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGa                     >  1:4645833/1‑93 (MQ=255)
                                                                 cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATa                   <  2:5705642/93‑1 (MQ=255)
                                                                 cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATa                   >  1:5961678/1‑93 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATaa                  >  1:1154843/1‑93 (MQ=255)
                                                                  gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATaa                  >  1:4527206/1‑93 (MQ=255)
                                                                   cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAg                 >  1:687110/1‑93 (MQ=255)
                                                                    tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGc                <  1:560367/93‑1 (MQ=255)
                                                                      tGTTTATGGCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCgg              <  2:2470912/93‑1 (MQ=255)
                                                                      tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCgg              <  2:6070596/93‑1 (MQ=255)
                                                                       gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGa             >  1:5666796/1‑93 (MQ=255)
                                                                       gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGa             <  1:5826418/93‑1 (MQ=255)
                                                                       gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGa             <  1:5424865/93‑1 (MQ=255)
                                                                       gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGa             <  1:4682460/93‑1 (MQ=255)
                                                                        tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAg            <  1:243611/93‑1 (MQ=255)
                                                                         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc           <  1:496888/93‑1 (MQ=255)
                                                                         ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgc           <  2:3449198/93‑1 (MQ=255)
                                                                          tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcg          <  2:3996697/93‑1 (MQ=255)
                                                                           aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcgc         <  2:1088643/93‑1 (MQ=255)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAGATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAgcgtc        >  2:4844099/1‑91 (MQ=14)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCa        >  1:4386798/1‑93 (MQ=255)
                                                                            tGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCa        <  1:3149371/93‑1 (MQ=255)
                                                                              ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATc      <  1:1798693/93‑1 (MQ=255)
                                                                              ccGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATc      <  2:2024311/93‑1 (MQ=255)
                                                                                ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg    >  2:2609059/1‑93 (MQ=255)
                                                                                ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg    >  2:170150/1‑93 (MQ=255)
                                                                                ggATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAg    <  2:4798849/93‑1 (MQ=255)
                                                                                 gATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAgg   <  1:756663/93‑1 (MQ=255)
                                                                                  aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGc  <  1:425940/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGCGGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGC  >  NC_000913/3703515‑3703689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGCGGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAT  >  NC_000913/3703509‑3703691
                                                                                             |                                                                                         
AAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGG                                                                                           <  SRR4302532.1771/94‑1 (MQ=60)
AAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGG                                                                                           <  SRR4302532.25049/94‑1 (MQ=60)
     TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTA                                                                                      >  SRR4302532.3511336/1‑94 (MQ=60)
       TCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAC                                                                                    <  SRR4302532.2041957/94‑1 (MQ=60)
       GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAC                                                                                    <  SRR4302532.6254120/94‑1 (MQ=60)
       GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAC                                                                                    <  SRR4302532.3193326/94‑1 (MQ=60)
       GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAC                                                                                    <  SRR4302532.4082226/94‑1 (MQ=60)
       GCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAC                                                                                    <  SRR4302532.5751110/94‑1 (MQ=60)
        CTGCCGGATGGGGCTGCACGCAGAGGAACAGGGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCCGATGCGGCGTTAACG                                                                                   >  SRR4302532.2121778/1‑94 (MQ=60)
        CTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACG                                                                                   <  SRR4302532.455009/94‑1 (MQ=60)
        CTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACG                                                                                   >  SRR4302532.1455707/1‑94 (MQ=60)
          GCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCC                                                                                 <  SRR4302532.746593/94‑1 (MQ=60)
          GCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCC                                                                                 <  SRR4302532.1624712/94‑1 (MQ=60)
           CCGGATGGCGCTGCACGCAGAGGGACGGCGTCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATTCCGGATGCGGCGTTAACGCCT                                                                                >  SRR4302532.1896071/1‑94 (MQ=54)
           CCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCT                                                                                <  SRR4302532.5641032/94‑1 (MQ=60)
           CCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCT                                                                                >  SRR4302532.1713174/1‑94 (MQ=60)
            CGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTT                                                                               >  SRR4302532.3891951/1‑94 (MQ=60)
            CGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTT                                                                               >  SRR4302532.4595425/1‑94 (MQ=60)
             GGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTA                                                                              >  SRR4302532.2804859/1‑94 (MQ=60)
             GGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTA                                                                              <  SRR4302532.1003806/94‑1 (MQ=60)
                TGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCC                                                                           <  SRR4302532.1511993/94‑1 (MQ=60)
                TGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCC                                                                           <  SRR4302532.5330872/94‑1 (MQ=60)
                 GGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCG                                                                          <  SRR4302532.5577045/94‑1 (MQ=60)
                   CGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGC                                                                        >  SRR4302532.1222567/1‑94 (MQ=60)
                     CTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCT                                                                      >  SRR4302532.1398601/1‑94 (MQ=60)
                     CTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCT                                                                      <  SRR4302532.2909941/94‑1 (MQ=60)
                     CTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCT                                                                      <  SRR4302532.4514659/94‑1 (MQ=60)
                      TGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTA                                                                     <  SRR4302532.5628892/94‑1 (MQ=60)
                       GCACGCGGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC                                                                    <  SRR4302532.428114/94‑1 (MQ=60)
                       GCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC                                                                    <  SRR4302532.2106879/94‑1 (MQ=60)
                       GCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC                                                                    <  SRR4302532.3365260/94‑1 (MQ=60)
                         ACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAA                                                                  >  SRR4302532.3940210/1‑94 (MQ=60)
                         ACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAA                                                                  <  SRR4302532.3746072/94‑1 (MQ=60)
                          CGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAA                                                                 >  SRR4302532.3000097/1‑94 (MQ=60)
                           GCAGAGGCACAGCGCCACCGGGGATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAA                                                                >  SRR4302532.6262537/1‑94 (MQ=60)
                            CAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAT                                                               >  SRR4302532.5172646/1‑94 (MQ=60)
                            CAGAGGCACAGCGCCACCGGGTATACAGATTGATGACAAAGTGCGCTTTGTTTGTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAAT                                                               <  SRR4302532.2384384/94‑1 (MQ=51)
                             AGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATC                                                              >  SRR4302532.3753879/1‑94 (MQ=58)
                             AGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATC                                                              <  SRR4302532.1246076/94‑1 (MQ=58)
                             AGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATC                                                              >  SRR4302532.3961185/1‑94 (MQ=58)
                             AGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATC                                                              <  SRR4302532.39559/94‑1 (MQ=58)
                             AGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATC                                                              <  SRR4302532.4833368/94‑1 (MQ=58)
                              GAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCG                                                             <  SRR4302532.6171001/94‑1 (MQ=55)
                              GAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCG                                                             >  SRR4302532.661835/1‑94 (MQ=55)
                               AGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGT                                                            >  SRR4302532.4780465/1‑94 (MQ=53)
                                GGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTG                                                           <  SRR4302532.5118460/94‑1 (MQ=51)
                                  CACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCA                                                         >  SRR4302532.5245820/1‑94 (MQ=46)
                                  CACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCA                                                         >  SRR4302532.981009/1‑94 (MQ=46)
                                  CACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCA                                                         >  SRR4302532.5773045/1‑94 (MQ=46)
                                   ACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAA                                                        >  SRR4302532.5285342/1‑94 (MQ=43)
                                   ACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAA                                                        <  SRR4302532.5045825/94‑1 (MQ=43)
                                     AGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAT                                                      >  SRR4302532.3858109/1‑94 (MQ=39)
                                      GCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATT                                                     <  SRR4302532.5850242/94‑1 (MQ=36)
                                       CGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTC                                                    <  SRR4302532.2300450/94‑1 (MQ=34)
                                       CGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTC                                                    >  SRR4302532.61826/1‑94 (MQ=34)
                                       CGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTC                                                    <  SRR4302532.57643/94‑1 (MQ=34)
                                       CGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTC                                                    <  SRR4302532.2528180/94‑1 (MQ=34)
                                        GCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA                                                   >  SRR4302532.6184419/1‑94 (MQ=31)
                                        GCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA                                                   >  SRR4302532.6201524/1‑94 (MQ=31)
                                        GCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCA                                                   <  SRR4302532.1974617/94‑1 (MQ=31)
                                          CACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAT                                                 >  SRR4302532.5379251/1‑94 (MQ=29)
                                          CACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAT                                                 >  SRR4302532.703211/1‑94 (MQ=29)
                                          CACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAT                                                 <  SRR4302532.545567/94‑1 (MQ=29)
                                          CACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAT                                                 <  SRR4302532.2204567/94‑1 (MQ=29)
                                           ACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATA                                                >  SRR4302532.2079631/1‑94 (MQ=29)
                                           ACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATA                                                <  SRR4302532.6322133/94‑1 (MQ=29)
                                           ACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATA                                                <  SRR4302532.1834640/94‑1 (MQ=29)
                                            CCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAC                                               <  SRR4302532.5427588/94‑1 (MQ=29)
                                             CGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACA                                              <  SRR4302532.2880950/94‑1 (MQ=29)
                                              GGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAT                                             >  SRR4302532.2605197/1‑94 (MQ=29)
                                               GGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATT                                            <  SRR4302532.287143/94‑1 (MQ=29)
                                               GGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATT                                            <  SRR4302532.3920584/94‑1 (MQ=29)
                                               GGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATT                                            <  SRR4302532.3920585/94‑1 (MQ=29)
                                                GTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTG                                           >  SRR4302532.3462858/1‑94 (MQ=29)
                                                GTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTG                                           <  SRR4302532.4197491/94‑1 (MQ=29)
                                                GTATACAGATTGTTGACAAAGCGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAgtc                                           >  SRR4302532.4404176/1‑91 (MQ=21)
                                                 TATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC                                          <  SRR4302532.3936789/94‑1 (MQ=29)
                                                 TATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGA                                          >  SRR4302532.1442868/1‑94 (MQ=26)
                                                  ATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA                                         >  SRR4302532.5602438/1‑94 (MQ=29)
                                                  ATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA                                         >  SRR4302532.3033530/1‑94 (MQ=29)
                                                  ATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA                                         <  SRR4302532.3646547/94‑1 (MQ=29)
                                                  ATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCA                                         <  SRR4302532.3303102/94‑1 (MQ=29)
                                                   TACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAG                                        >  SRR4302532.5158027/1‑94 (MQ=29)
                                                   TACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAG                                        >  SRR4302532.3174213/1‑94 (MQ=29)
                                                    ACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGA                                       >  SRR4302532.5947507/1‑94 (MQ=29)
                                                     CAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAA                                      <  SRR4302532.6270492/94‑1 (MQ=29)
                                                     CAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAA                                      <  SRR4302532.398978/94‑1 (MQ=29)
                                                     CAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAA                                      >  SRR4302532.6322862/1‑94 (MQ=29)
                                                     CAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAA                                      <  SRR4302532.398979/94‑1 (MQ=29)
                                                      AGATTGTTGACAAAGTTTGCTTTGTTTATGCTGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT                                     <  SRR4302532.2551622/94‑1 (MQ=27)
                                                       GATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT                                    >  SRR4302532.1567433/1‑94 (MQ=32)
                                                       GATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT                                    >  SRR4302532.1358627/1‑94 (MQ=34)
                                                        ATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAGATCGTGCAAATTCAATACATTGCAGAATTT                                   >  SRR4302532.6217490/1‑94 (MQ=60)
                                                        ATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTT                                   <  SRR4302532.6332442/94‑1 (MQ=34)
                                                        ATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTT                                   <  SRR4302532.582212/94‑1 (MQ=34)
                                                        ATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTT                                   >  SRR4302532.493614/1‑94 (MQ=34)
                                                        ATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGAGGCGTTAACGCATTATACGGCCTACAAAATCGTGCAAATTCAATACATTGCAGACTTT                                   >  SRR4302532.1755358/1‑94 (MQ=20)
                                                         TTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTT                                  >  SRR4302532.2153138/1‑94 (MQ=34)
                                                         TTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTT                                  >  SRR4302532.202413/1‑94 (MQ=34)
                                                          TGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTC                                 <  SRR4302532.3968784/94‑1 (MQ=34)
                                                           GTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCG                                >  SRR4302532.944935/1‑94 (MQ=34)
                                                            TTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGT                               >  SRR4302532.1391990/1‑94 (MQ=34)
                                                              GACAAATTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAG                             <  SRR4302532.908722/94‑1 (MQ=37)
                                                              GACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAG                             <  SRR4302532.33040/94‑1 (MQ=39)
                                                              GACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAG                             >  SRR4302532.6190456/1‑94 (MQ=39)
                                                               ACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGG                            <  SRR4302532.6089340/94‑1 (MQ=41)
                                                               ACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGG                            <  SRR4302532.764589/94‑1 (MQ=41)
                                                               ACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGG                            <  SRR4302532.1627544/94‑1 (MQ=41)
                                                               ACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGG                            <  SRR4302532.1728423/94‑1 (MQ=41)
                                                                CAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGC                           <  SRR4302532.5942018/94‑1 (MQ=43)
                                                                  AAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCT                         >  SRR4302532.1949788/1‑94 (MQ=43)
                                                                   AGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTG                        >  SRR4302532.405273/1‑94 (MQ=46)
                                                                    GTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGA                       >  SRR4302532.4804228/1‑94 (MQ=53)
                                                                      GCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATA                     >  SRR4302532.6167847/1‑94 (MQ=58)
                                                                       CGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAA                    >  SRR4302532.4681729/1‑94 (MQ=60)
                                                                       CGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAA                    >  SRR4302532.1193372/1‑94 (MQ=60)
                                                                        GCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAG                   >  SRR4302532.710678/1‑94 (MQ=60)
                                                                          TTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCG                 <  SRR4302532.579900/94‑1 (MQ=60)
                                                                           TTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGG                <  SRR4302532.2796545/94‑1 (MQ=60)
                                                                            TGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGA               >  SRR4302532.5864331/1‑94 (MQ=60)
                                                                             TTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGTCCTGATAAGCGGAG              <  SRR4302532.4842148/94‑1 (MQ=59)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAGAATCGTGCAAATTCAATACATTGCAGAATTTTCTTAGGCCTGATAAGCGGAG              <  SRR4302532.3526150/94‑1 (MQ=49)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAG              <  SRR4302532.4842149/94‑1 (MQ=60)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAG              <  SRR4302532.5612884/94‑1 (MQ=60)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAG              <  SRR4302532.3537244/94‑1 (MQ=60)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAG              >  SRR4302532.4717964/1‑94 (MQ=60)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAG              <  SRR4302532.6028729/94‑1 (MQ=60)
                                                                             GTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAG              <  SRR4302532.6028839/94‑1 (MQ=60)
                                                                              TTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGC             <  SRR4302532.252032/94‑1 (MQ=60)
                                                                              TTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGC             <  SRR4302532.3993275/94‑1 (MQ=60)
                                                                               TTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCG            <  SRR4302532.514259/94‑1 (MQ=60)
                                                                               TTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCG            >  SRR4302532.2828613/1‑94 (MQ=60)
                                                                                 ATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCA          >  SRR4302532.309028/1‑94 (MQ=60)
                                                                                 ATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCA          >  SRR4302532.3817961/1‑94 (MQ=60)
                                                                                 ATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCA          >  SRR4302532.3971232/1‑94 (MQ=60)
                                                                                 ATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCA          >  SRR4302532.4535878/1‑94 (MQ=60)
                                                                                 ATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGAAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCA          >  SRR4302532.885488/1‑94 (MQ=25)
                                                                                  TGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCAT         <  SRR4302532.3253862/94‑1 (MQ=60)
                                                                                  TGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGAGCAT         <  SRR4302532.998809/94‑1 (MQ=60)
                                                                                   GCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATC        <  SRR4302532.5022711/94‑1 (MQ=60)
                                                                                    CCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCA       >  SRR4302532.1214589/1‑94 (MQ=60)
                                                                                    CCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCA       <  SRR4302532.1858752/94‑1 (MQ=60)
                                                                                    CCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCA       <  SRR4302532.3706697/94‑1 (MQ=60)
                                                                                     CGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAG      <  SRR4302532.5091913/94‑1 (MQ=60)
                                                                                     CGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATACAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAG      <  SRR4302532.3890438/94‑1 (MQ=60)
                                                                                      GGATGCGGCGTTAACGCCTTATCCGGCCTGCAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGG     >  SRR4302532.3153665/1‑94 (MQ=49)
                                                                                      GGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGG     <  SRR4302532.3294781/94‑1 (MQ=60)
                                                                                      GGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGG     >  SRR4302532.112533/1‑94 (MQ=60)
                                                                                      GGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGG     >  SRR4302532.2802466/1‑94 (MQ=60)
                                                                                      GGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGG     >  SRR4302532.6030949/1‑94 (MQ=60)
                                                                                      GGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGG     <  SRR4302532.3790201/94‑1 (MQ=60)
                                                                                       GATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGC    <  SRR4302532.782593/94‑1 (MQ=60)
                                                                                        ATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCA   >  SRR4302532.159313/1‑94 (MQ=60)
                                                                                        ATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCA   <  SRR4302532.440691/94‑1 (MQ=60)
                                                                                         TGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAT  >  SRR4302532.30595/1‑94 (MQ=60)
                                                                                         TGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAT  <  SRR4302532.6264430/94‑1 (MQ=60)
                                                                                             |                                                                                         
AAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGCGGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATTTTCGTAGGCCTGATAAGCGGAGCGCATCAGGCAT  >  NC_000913/3703509‑3703691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑