Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,714,436 2 bp→GC coding (261‑262/660 nt) yiaD → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,714,4360CG99.6% 905.1 / NA 257N87K (AAC→AAG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (91/165);  minor base T (0/1);  total (91/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,714,4370GC99.6% 891.6 / NA 256V88L (GTG→CTG) yiaDputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (90/165);  minor base T (0/1);  total (90/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714349‑3714523
                                                                                       ||                                                                                      
 cgcgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTgcgc                                                                                   <  2:5180727/93‑1 (MQ=255)
   cgGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGGTGCGCGa                                                                                 >  2:3794527/1‑93 (MQ=255)
    ggTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAc                                                                                >  2:3667943/1‑93 (MQ=255)
    ggTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAc                                                                                <  2:3465498/93‑1 (MQ=255)
     gTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACa                                                                               <  1:535649/93‑1 (MQ=255)
     gTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACa                                                                               >  2:2883626/1‑93 (MQ=255)
      tAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaa                                                                              <  1:571638/93‑1 (MQ=255)
       aaaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaa                                                                             <  2:621604/93‑1 (MQ=255)
        aaGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACaaaa                                                                            >  1:3954783/1‑93 (MQ=255)
         aGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGTCGGCGTCGTTGGTGAGTACAATTAAGTGTTGGAAGCGAAGCTGCGTGACAAAAt                                                                           >  1:5484692/1‑93 (MQ=255)
          ggCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATg                                                                          <  2:4126896/93‑1 (MQ=255)
           gcgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc                                                                         <  2:1153053/93‑1 (MQ=255)
           gcgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgc                                                                         <  2:6053261/93‑1 (MQ=255)
            cgcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcg                                                                        <  2:1495707/93‑1 (MQ=255)
             gcgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATgcgc                                                                       <  1:2350290/93‑1 (MQ=255)
              cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  2:3344755/93‑1 (MQ=255)
              cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  1:4566295/93‑1 (MQ=255)
              cgcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGcgcg                                                                      <  1:275502/93‑1 (MQ=255)
               gcTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg                                                                     <  1:1062428/93‑1 (MQ=255)
               gcTGAGTGGCGCAGCAGCAGGCGCGGCCCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCgg                                                                     <  1:5229388/93‑1 (MQ=255)
                cTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGc                                                                    >  2:46110/1‑93 (MQ=255)
                 tGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa                                                                   >  1:839946/1‑93 (MQ=255)
                 tGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCa                                                                   >  2:275377/1‑93 (MQ=255)
                  gATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATTCGCGGCAc                                                                  <  1:771636/93‑1 (MQ=255)
                   aTTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACt                                                                 <  2:4370803/93‑1 (MQ=255)
                   aTTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACt                                                                 >  2:5097904/1‑93 (MQ=255)
                    ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                <  2:3856849/93‑1 (MQ=255)
                    ttGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTg                                                                <  2:1355229/93‑1 (MQ=255)
                     tGGCACAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTgg                                                               <  1:2124824/93‑1 (MQ=255)
                      ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              <  2:1615446/93‑1 (MQ=255)
                      ggCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGgt                                                              <  1:4229955/93‑1 (MQ=255)
                         gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt                                                           <  1:5800113/93‑1 (MQ=255)
                         gcagcagcaGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGtt                                                           <  1:1743463/93‑1 (MQ=255)
                          cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa                                                          <  2:2249979/93‑1 (MQ=255)
                          cagcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTa                                                          <  1:4802216/93‑1 (MQ=255)
                            gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAgt                                                        >  1:1108198/1‑92 (MQ=255)
                            gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  1:6089653/93‑1 (MQ=255)
                            gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  1:1635650/93‑1 (MQ=255)
                            gcagcagGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGc                                                        <  2:715210/93‑1 (MQ=255)
                              agcagGTGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTTGTGTTAGCGt                                                      <  1:5051691/93‑1 (MQ=255)
                                 agGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAc                                                   <  2:4899214/93‑1 (MQ=255)
                                  ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  <  1:4103827/93‑1 (MQ=255)
                                  ggCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAcc                                                  >  2:5550634/1‑93 (MQ=255)
                                     gcAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAATGGTGTTAGCGTAACCCGc                                               >  2:5229202/1‑93 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  2:2021186/93‑1 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  1:1074406/93‑1 (MQ=255)
                                      cAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCa                                              <  2:4994243/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGTGCGGCACTGGTGTTAGCGTAACCCGCAg                                             <  2:1823151/93‑1 (MQ=255)
                                       aGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAg                                             >  2:6123719/1‑93 (MQ=255)
                                        gCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGc                                            >  2:5700688/1‑93 (MQ=255)
                                          tctGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCgg                                          >  2:2665547/1‑93 (MQ=255)
                                            tGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGCTGTTAGCGTAACCCGCAGCgggg                                        >  1:3538347/1‑93 (MQ=255)
                                             gggCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGa                                       >  1:223823/1‑93 (MQ=255)
                                              ggcggcggcGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAt                                      <  2:4706138/93‑1 (MQ=255)
                                                  gcggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACa                                  <  2:2384074/93‑1 (MQ=255)
                                                    ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                <  2:1440593/93‑1 (MQ=255)
                                                    ggcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAtt                                <  2:4819125/93‑1 (MQ=255)
                                                     gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               <  1:3826744/93‑1 (MQ=255)
                                                     gcgTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTa                               <  1:5602384/93‑1 (MQ=255)
                                                       gTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc                             >  2:1012556/1‑93 (MQ=255)
                                                       gTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATc                             >  1:5062568/1‑93 (MQ=255)
                                                         tGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCt                           >  1:951103/1‑93 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          >  1:243882/1‑93 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          <  2:2294897/93‑1 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          <  1:2007679/93‑1 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          >  2:2726718/1‑93 (MQ=255)
                                                          ggTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTc                          <  1:5198055/93‑1 (MQ=255)
                                                           gTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCa                         >  2:2580784/1‑93 (MQ=255)
                                                            ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        >  2:5818515/1‑93 (MQ=255)
                                                            ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  1:1681776/93‑1 (MQ=255)
                                                            ttattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCaa                        <  2:3132261/93‑1 (MQ=255)
                                                             tattaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       <  1:4360409/93‑1 (MQ=255)
                                                             tagtaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAat                       <  1:3175653/90‑1 (MQ=255)
                                                              attaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAata                      <  1:2919438/93‑1 (MQ=255)
                                                               ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAatat                     <  1:263946/93‑1 (MQ=255)
                                                               ttaCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCAGGGATAACATTATCCTCAatat                     <  2:3106376/93‑1 (MQ=255)
                                                                taCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATg                    <  1:4476520/93‑1 (MQ=255)
                                                                taCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATg                    <  1:5970824/93‑1 (MQ=255)
                                                                 aCATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGc                   >  2:1917230/1‑93 (MQ=255)
                                                                     ggATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGaa               >  2:4464264/1‑93 (MQ=255)
                                                                      gATGTGCAGGAAGCGAATCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              <  1:3394811/93‑1 (MQ=255)
                                                                      gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              <  1:1222966/93‑1 (MQ=255)
                                                                      gATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAAc              <  1:188298/93‑1 (MQ=255)
                                                                        tgtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACaa            <  1:4685651/93‑1 (MQ=255)
                                                                         gtgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAt           >  2:3634202/1‑93 (MQ=255)
                                                                          tgCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAtg          <  1:3737707/93‑1 (MQ=255)
                                                                              ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      >  2:1762161/1‑93 (MQ=255)
                                                                              ggAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGGGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAc      >  2:1224546/1‑93 (MQ=255)
                                                                               gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     >  2:2955391/1‑93 (MQ=255)
                                                                               gaagcgaagcTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAcc     <  2:5942784/93‑1 (MQ=255)
                                                                                aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    >  1:4772351/1‑93 (MQ=255)
                                                                                aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  2:5328575/93‑1 (MQ=255)
                                                                                aaGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCCCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCt    <  1:127968/93‑1 (MQ=255)
                                                                                 aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   <  1:4649641/93‑1 (MQ=255)
                                                                                 aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   <  1:4892651/93‑1 (MQ=255)
                                                                                 aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   <  2:3287804/93‑1 (MQ=255)
                                                                                 aGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCtt   <  2:4490441/93‑1 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  2:2173690/93‑1 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  <  2:1748828/93‑1 (MQ=255)
                                                                                  gCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTc  >  1:4054446/1‑93 (MQ=255)
                                                                                       ||                                                                                      
TCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC  >  NC_000913/3714349‑3714523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714343‑3714525
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GAAAGATCGCGGTAAAGGCGCGCTTATTGGCGCAGGAGCAGGCGCAGCTGGGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           <  SRR4302532.2032745/94‑1 (MQ=60)
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           <  SRR4302532.2032746/94‑1 (MQ=60)
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           <  SRR4302532.3446998/94‑1 (MQ=60)
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAG                                                                                           <  SRR4302532.4879735/94‑1 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                      >  SRR4302532.6020285/1‑94 (MQ=60)
     ATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCG                                                                                      <  SRR4302532.4091459/94‑1 (MQ=60)
         CGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGAC                                                                                  >  SRR4302532.6051129/1‑94 (MQ=60)
           GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                <  SRR4302532.5471195/94‑1 (MQ=60)
           GTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAA                                                                                <  SRR4302532.554315/94‑1 (MQ=60)
            TAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAA                                                                               <  SRR4302532.591579/94‑1 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                              >  SRR4302532.4088053/1‑94 (MQ=60)
             AAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAA                                                                              >  SRR4302532.1119816/1‑94 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             >  SRR4302532.4195757/1‑94 (MQ=60)
              AAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAAT                                                                             <  SRR4302532.4107918/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            >  SRR4302532.5302703/1‑94 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302532.3334135/94‑1 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            >  SRR4302532.832395/1‑94 (MQ=60)
               AGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATG                                                                            <  SRR4302532.4753631/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        <  SRR4302532.2428699/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        <  SRR4302532.2428698/94‑1 (MQ=60)
                   GCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCG                                                                        <  SRR4302532.4970197/94‑1 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       <  SRR4302532.4722040/94‑1 (MQ=60)
                    CGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGG                                                                       <  SRR4302532.285045/94‑1 (MQ=60)
                     GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                      >  SRR4302532.6190433/1‑94 (MQ=60)
                     GCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                      <  SRR4302532.1098198/94‑1 (MQ=60)
                     GCTGAGTGGCGCAGCAGCAGGCGCGGCCCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGC                                                                      <  SRR4302532.5409732/94‑1 (MQ=60)
                      CTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCA                                                                     >  SRR4302532.868764/1‑94 (MQ=60)
                      CTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCA                                                                     <  SRR4302532.1099898/94‑1 (MQ=60)
                       TGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCAC                                                                    >  SRR4302532.4244280/1‑94 (MQ=60)
                        GATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATTCGCGGCACT                                                                   <  SRR4302532.798261/94‑1 (MQ=60)
                         ATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTG                                                                  <  SRR4302532.51748/94‑1 (MQ=60)
                          TTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGG                                                                 <  SRR4302532.1434938/94‑1 (MQ=60)
                           TGGCACAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGT                                                                <  SRR4302532.2195815/94‑1 (MQ=60)
                            GGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTG                                                               <  SRR4302532.4980607/94‑1 (MQ=60)
                            GGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTG                                                               <  SRR4302532.4371951/94‑1 (MQ=60)
                             GCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGT                                                              >  SRR4302532.5355144/1‑94 (MQ=60)
                               GCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTA                                                            <  SRR4302532.1802120/94‑1 (MQ=60)
                               GCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTA                                                            <  SRR4302532.6001686/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           <  SRR4302532.4967608/94‑1 (MQ=60)
                                CAGCAGCAGGCGCAGCTCTGGGCGACGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAG                                                           <  SRR4302532.920615/94‑1 (MQ=60)
                                 AGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGT                                                          >  SRR4302532.1145256/1‑94 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302532.1691015/94‑1 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302532.4943180/94‑1 (MQ=60)
                                  GCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCG                                                         <  SRR4302532.6299892/94‑1 (MQ=60)
                                   CAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGT                                                        <  SRR4302532.5958344/94‑1 (MQ=60)
                                    AGCAGGTGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTTGTGTTAGCGTA                                                       <  SRR4302532.5225041/94‑1 (MQ=60)
                                    AGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTA                                                       >  SRR4302532.2251095/1‑94 (MQ=60)
                                      CAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAAC                                                     >  SRR4302532.3285259/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTACCC                                                    >  SRR4302532.898190/1‑94 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACC                                                    <  SRR4302532.5867126/94‑1 (MQ=60)
                                       AGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGGGTTAGCGTAACC                                                    >  SRR4302532.1318582/1‑94 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                   <  SRR4302532.4241562/94‑1 (MQ=60)
                                        GGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCC                                                   <  SRR4302532.6280212/94‑1 (MQ=60)
                                         GCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCG                                                  <  SRR4302532.4223060/94‑1 (MQ=60)
                                           GCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCA                                                >  SRR4302532.4541845/1‑94 (MQ=60)
                                           GCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCA                                                <  SRR4302532.1128156/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302532.1110471/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302532.4823724/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302532.5548477/94‑1 (MQ=60)
                                            CAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAG                                               <  SRR4302532.2354231/94‑1 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              >  SRR4302532.3531422/1‑94 (MQ=60)
                                             AGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGC                                              <  SRR4302532.403679/94‑1 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                           <  SRR4302532.644175/94‑1 (MQ=60)
                                                TCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGG                                           >  SRR4302532.4816558/1‑94 (MQ=60)
                                                 CTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGCTGTTAGCGTAACCCGCAGCGGGG                                          >  SRR4302532.3656728/1‑94 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         >  SRR4302532.5394758/1‑94 (MQ=60)
                                                  TGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGA                                         >  SRR4302532.231468/1‑94 (MQ=60)
                                                     GGGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGTTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAA                                      <  SRR4302532.1766164/94‑1 (MQ=60)
                                                     GCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAA                                      <  SRR4302532.860992/94‑1 (MQ=60)
                                                      CGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAAC                                     <  SRR4302532.5569724/94‑1 (MQ=60)
                                                       GGCGGAGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACA                                    >  SRR4302532.277687/1‑94 (MQ=60)
                                                        GCGGCGTTGGTTATTACATAGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACAT                                   <  SRR4302532.5858425/94‑1 (MQ=60)
                                                         CGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATT                                  >  SRR4302532.2017653/1‑94 (MQ=60)
                                                         CGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATT                                  <  SRR4302532.4910074/94‑1 (MQ=60)
                                                           GCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAT                                <  SRR4302532.5796853/94‑1 (MQ=60)
                                                           GCGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTAT                                <  SRR4302532.3955937/94‑1 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               >  SRR4302532.5236381/1‑94 (MQ=60)
                                                            CGTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATC                               >  SRR4302532.3019972/1‑94 (MQ=60)
                                                             GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              >  SRR4302532.1180109/1‑94 (MQ=60)
                                                             GTTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCC                              >  SRR4302532.1713353/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302532.983609/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             >  SRR4302532.4712280/1‑94 (MQ=60)
                                                              TTGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCT                             <  SRR4302532.3158851/94‑1 (MQ=60)
                                                               TGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTC                            >  SRR4302532.252318/1‑94 (MQ=60)
                                                               TGGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTC                            <  SRR4302532.962365/94‑1 (MQ=60)
                                                                GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                           <  SRR4302532.2074640/94‑1 (MQ=60)
                                                                GGTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCA                           <  SRR4302532.5377257/94‑1 (MQ=60)
                                                                 GTTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAA                          >  SRR4302532.5781283/1‑94 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         <  SRR4302532.4723501/94‑1 (MQ=60)
                                                                  TTATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAAT                         <  SRR4302532.1738612/94‑1 (MQ=60)
                                                                  TGATTACATGGATGTGCAGGAAGGGAGGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGAAAACATTATCCTCAAT                         <  SRR4302532.4427905/94‑1 (MQ=60)
                                                                   TATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        <  SRR4302532.4508162/94‑1 (MQ=60)
                                                                   TAGTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATA                        <  SRR4302532.3281115/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302532.1027676/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302532.3016669/94‑1 (MQ=60)
                                                                    ATTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATAT                       <  SRR4302532.368447/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                      <  SRR4302532.273104/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                      <  SRR4302532.5766904/94‑1 (MQ=60)
                                                                     TTACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATG                      <  SRR4302532.523648/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     <  SRR4302532.4929411/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     <  SRR4302532.6177258/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     <  SRR4302532.5403835/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     <  SRR4302532.410547/94‑1 (MQ=60)
                                                                      TACATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGC                     <  SRR4302532.4629228/94‑1 (MQ=60)
                                                                         ATGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGA                  >  SRR4302532.1966371/1‑94 (MQ=60)
                                                                          TGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAA                 >  SRR4302532.3711273/1‑94 (MQ=60)
                                                                          TGGATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAA                 <  SRR4302532.4543123/94‑1 (MQ=60)
                                                                            GATGTGCAGGAAGCGAATCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302532.3508394/94‑1 (MQ=60)
                                                                            GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302532.1263873/94‑1 (MQ=60)
                                                                            GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302532.3508396/94‑1 (MQ=60)
                                                                            GATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACA               <  SRR4302532.194627/94‑1 (MQ=60)
                                                                             ATGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAA              <  SRR4302532.1925327/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302532.2514431/94‑1 (MQ=60)
                                                                              TGTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302532.4845464/94‑1 (MQ=60)
                                                                              TGTGCAGAAAGCGACGCTGCGCGACAAAATGCGCGGTAGTGGTGTTAGCGTATCCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAAT             <  SRR4302532.2514430/94‑1 (MQ=60)
                                                                               GTGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATG            <  SRR4302532.2172396/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302532.3863294/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302532.2130395/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGT           <  SRR4302532.1473932/94‑1 (MQ=60)
                                                                                TGCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATCT           >  SRR4302532.3896675/1‑94 (MQ=60)
                                                                                 GCAGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTG          <  SRR4302532.1260503/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCCCTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302532.5223549/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302532.3997221/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        <  SRR4302532.2813587/94‑1 (MQ=60)
                                                                                   AGGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGAC        >  SRR4302532.2652562/1‑94 (MQ=60)
                                                                                    GGAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACC       >  SRR4302532.1004389/1‑94 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302532.2983269/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302532.193218/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302532.4415594/94‑1 (MQ=60)
                                                                                     GAAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      >  SRR4302532.4936563/1‑94 (MQ=60)
                                                                                     GAAGCGAAGCGGCGCGACAATATGCTCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCT      <  SRR4302532.2886413/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302532.1693064/94‑1 (MQ=60)
                                                                                      AAGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCCCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTT     <  SRR4302532.132292/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    >  SRR4302532.3261992/1‑94 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    >  SRR4302532.4190814/1‑94 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    <  SRR4302532.5060782/94‑1 (MQ=60)
                                                                                       AGCGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTC    <  SRR4302532.4808170/94‑1 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  SRR4302532.5811242/1‑94 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  SRR4302532.54789/1‑94 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  SRR4302532.1903260/1‑94 (MQ=60)
                                                                                         CGAAGCTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  <  SRR4302532.1242294/94‑1 (MQ=60)
                                                                                             |                                                                                         
GAAAGATCGCGGTAAAGGCGCGCTGATTGGCGCAGCAGCAGGCGCAGCTCTGGGCGGCGGCGTTGGTTATTACATGGATGTGCAGGAAGCGAACGTGCGCGACAAAATGCGCGGCACTGGTGTTAGCGTAACCCGCAGCGGGGATAACATTATCCTCAATATGCCGAACAATGTGACCTTCGA  >  NC_000913/3714343‑3714525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑