Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,9560GC100.0% 895.0 / NA 250G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (123/127);  total (123/127)
*NC_0009133,737,9570CG100.0% 905.1 / NA 250G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (123/127);  total (123/127)

Only 100 of 257 total aligned reads displayed.

AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043
                                                                                       ||                                                                                      
  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  <  1:4545075/93‑1 (MQ=255)
  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  <  2:2671791/93‑1 (MQ=255)
   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 >  1:4594519/1‑93 (MQ=255)
    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGCCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  1:929146/93‑1 (MQ=255)
    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  2:3260363/93‑1 (MQ=255)
    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  2:64757/93‑1 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  2:882764/1‑93 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  2:2937664/93‑1 (MQ=255)
      ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              >  2:6122329/1‑93 (MQ=255)
        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc                                                                            <  1:625468/93‑1 (MQ=255)
        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc                                                                            <  1:3070626/93‑1 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  1:5813322/1‑93 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  1:5684309/1‑93 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  2:2258744/1‑93 (MQ=255)
         tcctttGCGCATCCCGGCCGACAAAACCAACCGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           <  1:5237006/90‑1 (MQ=255)
          tatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          <  1:1290426/92‑1 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          >  2:2573004/1‑93 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTGGACGCGCTGGCGAATCAACACGGCAt                                                                          >  2:405100/1‑93 (MQ=255)
           atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTTACGCGCTGGCGAATCAACACGGCAtt                                                                         >  2:1632643/1‑93 (MQ=255)
           atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt                                                                         <  1:1732370/93‑1 (MQ=255)
           atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt                                                                         >  1:1249570/1‑93 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        >  2:2477546/1‑93 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  1:5423296/93‑1 (MQ=255)
             ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       <  1:383913/93‑1 (MQ=255)
             ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       <  1:1466626/93‑1 (MQ=255)
              tGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg                                                                      >  1:2541482/1‑93 (MQ=255)
               gCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc                                                                     <  1:1966813/93‑1 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg                                                                   <  1:1628765/93‑1 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg                                                                   >  2:557764/1‑93 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga                                                               <  2:5457959/93‑1 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga                                                               <  1:6005365/93‑1 (MQ=255)
                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              >  1:1551/1‑93 (MQ=255)
                       cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt                                                             <  2:162192/93‑1 (MQ=255)
                       cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt                                                             <  2:5910/93‑1 (MQ=255)
                        ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattg                                                            >  1:709927/1‑93 (MQ=255)
                         gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAATCCGCTGGtgattga                                                           >  2:1292406/1‑93 (MQ=255)
                          ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          <  1:2766898/93‑1 (MQ=255)
                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:596288/1‑93 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  1:112458/1‑93 (MQ=255)
                            cacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        <  1:4649345/92‑1 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  2:2595090/93‑1 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  2:2467056/93‑1 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       >  2:188099/1‑93 (MQ=255)
                              cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  1:5529709/1‑93 (MQ=255)
                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  2:3303210/1‑93 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:5806405/93‑1 (MQ=255)
                                  accAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:4231811/92‑1 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 >  1:1070373/1‑93 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 <  2:5793838/93‑1 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 >  1:6083732/1‑93 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 >  2:2390397/1‑93 (MQ=255)
                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                <  1:5709603/93‑1 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  2:5732084/1‑93 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  1:2797498/1‑93 (MQ=255)
                                        aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt                                            >  2:4428431/1‑93 (MQ=255)
                                        aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt                                            >  1:3351090/1‑93 (MQ=255)
                                          gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc                                          <  2:1103169/93‑1 (MQ=255)
                                            aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg                                        >  2:4465471/1‑93 (MQ=255)
                                             aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt                                       >  2:3592093/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  2:1364276/1‑93 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    >  1:3573054/1‑93 (MQ=255)
                                                 gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   <  2:746531/93‑1 (MQ=255)
                                                 gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   >  2:2043456/1‑93 (MQ=255)
                                                 gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   >  2:4746038/1‑93 (MQ=255)
                                                  aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg                                  <  1:5038310/93‑1 (MQ=255)
                                                  aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGCGATTGATAACGCTTATGGCGTCCCGTTCCCg                                  >  2:787833/1‑93 (MQ=255)
                                                   tttcTGACGAAGAGTTTCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 <  1:305345/90‑1 (MQ=255)
                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 >  2:3802916/1‑93 (MQ=255)
                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 <  2:4514356/93‑1 (MQ=255)
                                                     aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               >  1:4137009/1‑93 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  1:5951364/93‑1 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  1:4327938/93‑1 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  2:1795330/93‑1 (MQ=255)
                                                        gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatc                            <  2:4667234/93‑1 (MQ=255)
                                                         aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca                           >  1:3106225/1‑93 (MQ=255)
                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         >  2:4897137/1‑93 (MQ=255)
                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         >  2:4366026/1‑93 (MQ=255)
                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  1:6144037/93‑1 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        >  2:4574829/1‑93 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        <  2:5158112/93‑1 (MQ=255)
                                                             agagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCtt                       >  2:4514611/1‑93 (MQ=255)
                                                              gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc                      >  2:5226632/1‑93 (MQ=255)
                                                              gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc                      >  2:1091350/1‑93 (MQ=255)
                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTGCCGTTCCCGGGGATCATCTTCAGTGa                 >  1:4525038/1‑93 (MQ=255)
                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  2:2725600/93‑1 (MQ=255)
                                                                    cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  1:1790225/93‑1 (MQ=255)
                                                                    cTGAAGCGTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  1:2125689/93‑1 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:2293297/93‑1 (MQ=255)
                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           <  1:5117029/93‑1 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         <  2:2879208/93‑1 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         >  1:3806135/1‑93 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         >  2:5645255/1‑93 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         <  1:4960940/93‑1 (MQ=255)
                                                                            tGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGccc        >  2:5082721/1‑93 (MQ=255)
                                                                              aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      <  2:2500621/93‑1 (MQ=255)
                                                                               cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCt     <  1:5326520/93‑1 (MQ=255)
                                                                               cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCt     >  1:5635112/1‑93 (MQ=255)
                                                                                gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    >  2:36332/1‑93 (MQ=255)
                                                                                 cgcTGGCGAATCAACACGGCGTTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTGATATCTTCAGTGAAGAGCGCCCGCTAt   >  1:68954/1‑93 (MQ=255)
                                                                                  gcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  <  2:4253772/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737863‑3738047
                                                                                             |                                                                                            
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGC                                                                                              <  SRR4302532.376636/94‑1 (MQ=60)
       AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      <  SRR4302532.3404027/94‑1 (MQ=60)
        ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     <  SRR4302532.4700213/94‑1 (MQ=60)
        ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     >  SRR4302532.4751141/1‑94 (MQ=60)
        ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     <  SRR4302532.4081163/94‑1 (MQ=60)
         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    >  SRR4302532.4047259/1‑94 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGCCGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   <  SRR4302532.960972/94‑1 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   <  SRR4302532.4736820/94‑1 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGGAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302532.1325754/1‑94 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302532.3440675/1‑94 (MQ=60)
            GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 <  SRR4302532.4847288/94‑1 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               >  SRR4302532.5882733/1‑94 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               >  SRR4302532.6015266/1‑94 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302532.647320/94‑1 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302532.3172083/94‑1 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302532.4698726/94‑1 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               >  SRR4302532.5188163/1‑94 (MQ=60)
               tcctttgcgcaTCCCGGCCGACAAAACCAACCGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302532.5417587/83‑1 (MQ=60)
                TATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             <  SRR4302532.1333604/94‑1 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             >  SRR4302532.1291385/1‑94 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            <  SRR4302532.1790682/94‑1 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302532.294484/1‑94 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302532.664892/1‑94 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302532.5611262/94‑1 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302532.5557316/94‑1 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302532.1199558/94‑1 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302532.1817783/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          <  SRR4302532.3706120/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          <  SRR4302532.1516033/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          >  SRR4302532.2626242/1‑94 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          <  SRR4302532.397335/94‑1 (MQ=60)
                    TGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGC                                                                         >  SRR4302532.3938546/1‑94 (MQ=60)
                     GCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCT                                                                        <  SRR4302532.2032144/94‑1 (MQ=60)
                      CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTG                                                                       <  SRR4302532.5885148/94‑1 (MQ=60)
                       GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      <  SRR4302532.1683899/94‑1 (MQ=60)
                        TCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGT                                                                     <  SRR4302532.5006729/94‑1 (MQ=60)
                         CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302532.4516289/1‑94 (MQ=60)
                          TCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGA                                                                   >  SRR4302532.4722074/1‑94 (MQ=60)
                           CCTGGCCGCCGAATCGAACAGACAATGTGATTACTGACGAAGAGTTGCTGAAGCTTCACGCGCTGTCG‑AATCAACACGGCATTCAGCTGGTGAT                                                                  <  SRR4302532.1925950/94‑1 (MQ=57)
                           CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  <  SRR4302532.6212743/94‑1 (MQ=60)
                           CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302532.6256356/1‑94 (MQ=60)
                           CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302532.1609/1‑94 (MQ=60)
                           CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302532.1377492/1‑94 (MQ=60)
                             CGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTG                                                                >  SRR4302532.734185/1‑94 (MQ=60)
                               GCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGAT                                                              <  SRR4302532.1807829/94‑1 (MQ=60)
                               GCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGAT                                                              >  SRR4302532.5634169/1‑94 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             <  SRR4302532.2859800/94‑1 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             <  SRR4302532.2784679/94‑1 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302532.5282949/1‑94 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302532.6075201/1‑94 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302532.4567229/1‑94 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302532.116325/1‑94 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302532.116326/1‑94 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302532.5786902/1‑94 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302532.587507/1‑94 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302532.5577238/94‑1 (MQ=60)
                                  CACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302532.4807859/94‑1 (MQ=60)
                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          >  SRR4302532.5721607/1‑94 (MQ=60)
                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACCGGATTCCGCTGGTGACTGATAACG                                                          >  SRR4302532.803889/1‑94 (MQ=60)
                                    CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                         >  SRR4302532.541530/1‑94 (MQ=60)
                                     GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                        <  SRR4302532.1883762/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302532.5154473/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     >  SRR4302532.1106334/1‑94 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     >  SRR4302532.1722577/1‑94 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302532.2015275/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302532.5306476/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     >  SRR4302532.6293808/1‑94 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302532.6008153/94‑1 (MQ=60)
                                        ACCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302532.4373874/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302532.6169225/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302532.4310156/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302532.3668234/94‑1 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302532.5973478/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   <  SRR4302532.5908639/94‑1 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302532.3164278/1‑94 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  >  SRR4302532.2891256/1‑94 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302532.691423/94‑1 (MQ=60)
                                            ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 >  SRR4302532.5879767/1‑94 (MQ=60)
                                            ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 >  SRR4302532.3174385/1‑94 (MQ=60)
                                             CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                >  SRR4302532.3462970/1‑94 (MQ=60)
                                               GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              <  SRR4302532.393267/94‑1 (MQ=60)
                                                GCAATGTTATTACTGACGAAGAGTTGCTGCAGCTTGCCGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                             <  SRR4302532.5839890/94‑1 (MQ=60)
                                                GCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                             >  SRR4302532.73473/1‑94 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            <  SRR4302532.964738/94‑1 (MQ=60)
                                                  AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           >  SRR4302532.1628056/1‑94 (MQ=60)
                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302532.3692616/1‑94 (MQ=60)
                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGCTCC                                        >  SRR4302532.2214948/1‑94 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     <  SRR4302532.5211119/94‑1 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     <  SRR4302532.6308685/94‑1 (MQ=60)
                                                         TTTCTGACGAAGAGTTTCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    <  SRR4302532.315935/94‑1 (MQ=60)
                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    <  SRR4302532.315936/94‑1 (MQ=60)
                                                          TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                   >  SRR4302532.4275781/1‑94 (MQ=60)
                                                           TCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  <  SRR4302532.5973861/94‑1 (MQ=60)
                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCA                                  >  SRR4302532.2194157/1‑94 (MQ=60)
                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGCGATTGATAACGCTTATAGCGTCCCGTTCCCCGGTA                                  >  SRR4302532.2810122/1‑94 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302532.6332074/1‑94 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302532.4474168/94‑1 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302532.6157284/94‑1 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302532.3434036/1‑94 (MQ=60)
                                                              GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               >  SRR4302532.3209246/1‑94 (MQ=60)
                                                                CGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                             >  SRR4302532.1595037/1‑94 (MQ=60)
                                                                 GAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                            <  SRR4302532.6355960/94‑1 (MQ=60)
                                                                  AAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                           >  SRR4302532.4324902/1‑94 (MQ=60)
                                                                  AAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                           >  SRR4302532.1653255/1‑94 (MQ=60)
                                                                   AGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTC                          <  SRR4302532.2301736/94‑1 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        <  SRR4302532.1165431/94‑1 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTACCCGGGTATCATCTTCAG                        >  SRR4302532.2888526/1‑94 (MQ=60)
                                                                       TTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTG                      <  SRR4302532.1469743/94‑1 (MQ=60)
                                                                       TTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTG                      >  SRR4302532.4390220/1‑94 (MQ=60)
                                                                        TGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTGCCGTTCCCGGGGATCATCTTCAGTGA                     >  SRR4302532.4679492/1‑94 (MQ=60)
                                                                         GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    <  SRR4302532.939152/94‑1 (MQ=60)
                                                                         GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    >  SRR4302532.6236564/1‑94 (MQ=60)
                                                                          CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   >  SRR4302532.2655133/1‑94 (MQ=60)
                                                                          CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   <  SRR4302532.1850045/94‑1 (MQ=60)
                                                                          CTGAAGCGTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   <  SRR4302532.2196710/94‑1 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGGATCATCTTCAGTGAAGC                  >  SRR4302532.2560942/1‑94 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302532.6009101/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302532.639439/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302532.2369417/94‑1 (MQ=60)
                                                                               GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              <  SRR4302532.5293004/94‑1 (MQ=60)
                                                                                CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC             >  SRR4302532.3934624/1‑94 (MQ=60)
                                                                                 TTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCC            <  SRR4302532.5131095/94‑1 (MQ=60)
                                                                                    ACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCT         <  SRR4302532.6091731/94‑1 (MQ=60)
                                                                                    ACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCT         >  SRR4302532.5830843/1‑94 (MQ=60)
                                                                                     CGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA        <  SRR4302532.5511159/94‑1 (MQ=60)
                                                                                      GCGCTGGCG‑AATCAACACGGCGTTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTGATATCTTCAGTGAAGAGCGCCCGCTAT       >  SRR4302532.71427/1‑94 (MQ=60)
                                                                                      GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302532.4788081/94‑1 (MQ=60)
                                                                                      GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTAATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302532.2900400/94‑1 (MQ=60)
                                                                                       CGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG      <  SRR4302532.5803685/94‑1 (MQ=60)
                                                                                        GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302532.5498860/94‑1 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302532.3974847/1‑94 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    <  SRR4302532.1892248/94‑1 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302532.6020509/1‑94 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302532.5952656/1‑94 (MQ=60)
                                                                                            GGCGAATCACCACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  <  SRR4302532.2799334/94‑1 (MQ=60)
                                                                                            GGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  <  SRR4302532.4607992/94‑1 (MQ=60)
                                                                                             |                                                                                            
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737863‑3738047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑