Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,9560GC100.0% 908.6 / NA 246G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (132/114);  total (132/114)
*NC_0009133,737,9570CG99.6% 909.2 / NA 246G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (132/113);  total (132/114)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043
                                                                                       ||                                                                                      
aaaCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATc                                                                                    >  2:5518639/1‑93 (MQ=255)
  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  >  2:4165649/1‑93 (MQ=255)
  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  >  1:4104013/1‑93 (MQ=255)
  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  <  2:5200195/93‑1 (MQ=255)
   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 >  2:5774224/1‑93 (MQ=255)
   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  1:2996080/93‑1 (MQ=255)
    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  2:3592298/93‑1 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  1:5183704/1‑93 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  1:900164/93‑1 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  1:5770068/93‑1 (MQ=255)
      ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              <  2:4739034/93‑1 (MQ=255)
      ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              >  1:4807068/1‑93 (MQ=255)
        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc                                                                            <  2:2844087/93‑1 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  1:2693704/1‑93 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           <  1:3690996/93‑1 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          >  2:2943728/1‑93 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          <  2:2719081/93‑1 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          >  2:141886/1‑93 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  1:4856198/93‑1 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  1:5109215/93‑1 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  2:6309094/93‑1 (MQ=255)
             ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       >  2:717120/1‑93 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg                                                                   >  2:3999133/1‑93 (MQ=255)
                 gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg                                                                   >  2:312311/1‑93 (MQ=255)
                   ctcCCGGCCGACGTATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt                                                                 <  2:3790171/93‑1 (MQ=255)
                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt                                                                 <  1:5960053/93‑1 (MQ=255)
                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTAGCGAATCAACACGGCATTCCGCTggc                                                                 >  2:2612394/1‑92 (MQ=255)
                    tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAAGACGGCATTCCGCTGGtg                                                                >  1:6543393/1‑93 (MQ=255)
                    tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg                                                                >  2:5392621/1‑93 (MQ=255)
                    tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg                                                                <  2:3823554/93‑1 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga                                                               >  1:2272279/1‑93 (MQ=255)
                     cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga                                                               >  2:3963830/1‑93 (MQ=255)
                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              >  1:3438710/1‑93 (MQ=255)
                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              <  2:5107751/93‑1 (MQ=255)
                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              <  1:1231502/93‑1 (MQ=255)
                       cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt                                                             >  1:111267/1‑93 (MQ=255)
                           cggcgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:4102422/4‑93 (MQ=255)
                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  2:2031210/1‑93 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       >  1:4927081/1‑93 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  2:4251985/93‑1 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       >  1:3723848/1‑93 (MQ=255)
                             acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  1:2456524/93‑1 (MQ=255)
                              cgaATCCAACAGGCAATGTGATTACTGACGAAGAGCTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  1:6230246/1‑93 (MQ=255)
                                aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCTCTGGTGATTGATAACGCt                                                    <  1:3117368/93‑1 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  >  1:2936962/1‑93 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  2:623352/93‑1 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 <  1:548139/93‑1 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 >  2:1661281/1‑93 (MQ=255)
                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                >  2:5875516/1‑93 (MQ=255)
                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                >  2:6104302/1‑93 (MQ=255)
                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                <  2:1630112/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:2396091/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               >  2:3778147/1‑93 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:5914358/93‑1 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATggt                                              >  1:4449529/1‑92 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  2:3235891/93‑1 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  2:2257641/1‑93 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  1:4706401/93‑1 (MQ=255)
                                            aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg                                        >  2:2730801/1‑93 (MQ=255)
                                            aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg                                        <  1:5415821/93‑1 (MQ=255)
                                             aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt                                       >  1:6345615/1‑93 (MQ=255)
                                              tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  2:3308882/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  2:5985510/1‑93 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGGATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    >  1:5942699/1‑93 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    <  1:704930/93‑1 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    >  1:4188865/1‑93 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    >  1:103111/1‑93 (MQ=255)
                                                  aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg                                  <  2:4460131/93‑1 (MQ=255)
                                                  aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg                                  <  2:1014788/93‑1 (MQ=255)
                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 <  2:5552572/93‑1 (MQ=255)
                                                     aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               <  1:4502476/93‑1 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  1:1994526/93‑1 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  2:543240/93‑1 (MQ=255)
                                                       tGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTat                             >  1:6005177/1‑93 (MQ=255)
                                                         aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca                           >  1:2161407/1‑93 (MQ=255)
                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  1:1857850/93‑1 (MQ=255)
                                                           gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  2:6515926/93‑1 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        >  2:2000607/1‑93 (MQ=255)
                                                               agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     <  2:1365239/93‑1 (MQ=255)
                                                               agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     >  1:5651153/1‑93 (MQ=255)
                                                               agTTGCTGAAGCTTAACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa                     <  2:2460974/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  2:3379274/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    >  2:856532/1‑93 (MQ=255)
                                                                  tGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTg                  <  2:2891734/93‑1 (MQ=255)
                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  2:4963872/93‑1 (MQ=255)
                                                                    cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                >  1:1949621/1‑93 (MQ=255)
                                                                    cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  2:5129975/93‑1 (MQ=255)
                                                                     tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  1:1765849/93‑1 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         <  1:2643219/93‑1 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGGATCATCTTCAGTGAAGCGCGcc         >  1:2760956/1‑93 (MQ=255)
                                                                             gACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCg       >  1:5027223/1‑93 (MQ=255)
                                                                             gACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCg       <  2:5640289/93‑1 (MQ=255)
                                                                             gACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCg       >  1:3519249/1‑93 (MQ=255)
                                                                             gACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCg       >  2:660171/1‑93 (MQ=255)
                                                                              aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      <  2:2987307/93‑1 (MQ=255)
                                                                              aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      >  2:1229789/1‑93 (MQ=255)
                                                                              aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      >  1:1515616/1‑93 (MQ=255)
                                                                                gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    <  1:2800434/93‑1 (MQ=255)
                                                                                 cgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   <  2:5246553/93‑1 (MQ=255)
                                                                                 cgcTGGCGAATCAACACGCCATTCCGCTGGTGATTGATAACGCTTATGGCGCCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   <  1:3156732/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737868‑3738047
                                                                                        |                                                                                            
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATC                                                                                        >  SRR4302547.3280575/1‑94 (MQ=60)
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATC                                                                                        <  SRR4302547.3056998/94‑1 (MQ=60)
  AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      >  SRR4302547.4187447/1‑94 (MQ=60)
  AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      >  SRR4302547.2788472/1‑94 (MQ=60)
    CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    >  SRR4302547.6083630/1‑94 (MQ=60)
    CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302547.3055687/94‑1 (MQ=60)
     CGTGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   <  SRR4302547.1733075/94‑1 (MQ=60)
     CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAGAC                                                                                   >  SRR4302547.192237/1‑94 (MQ=60)
     CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   >  SRR4302547.5718539/1‑94 (MQ=60)
     CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   >  SRR4302547.5290385/1‑94 (MQ=60)
      GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302547.917488/94‑1 (MQ=60)
      GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302547.5889407/94‑1 (MQ=60)
      GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302547.4906694/1‑94 (MQ=60)
      GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302547.3030797/1‑94 (MQ=60)
       GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 <  SRR4302547.920984/94‑1 (MQ=60)
       GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 >  SRR4302547.271821/1‑94 (MQ=60)
        GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGC                                                                                <  SRR4302547.6702135/94‑1 (MQ=60)
         ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302547.655116/94‑1 (MQ=60)
         ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               >  SRR4302547.2747784/1‑94 (MQ=60)
          TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              >  SRR4302547.5740734/1‑94 (MQ=60)
          TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302547.3766348/94‑1 (MQ=60)
           GATTTCCGTCTCCCGGCGGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGCCGCGCTGGCA‑AATCAACACGGCATT                                                                             <  SRR4302547.1773219/94‑1 (MQ=60)
            ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            <  SRR4302547.639856/94‑1 (MQ=60)
             TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302547.4956811/94‑1 (MQ=60)
             TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302547.5214504/94‑1 (MQ=60)
             TTTGCGTCGCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302547.5887499/94‑1 (MQ=60)
                GCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCT                                                                        <  SRR4302547.2635635/94‑1 (MQ=60)
                GCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCT                                                                        <  SRR4302547.5236172/94‑1 (MQ=60)
                   TCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGT                                                                     <  SRR4302547.429280/94‑1 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAGACGGCATTCCGCTGGTG                                                                    >  SRR4302547.6679581/1‑94 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTcgcg                                                                    >  SRR4302547.547896/1‑90 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    <  SRR4302547.5176889/94‑1 (MQ=60)
                    CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    <  SRR4302547.6083640/94‑1 (MQ=60)
                     TCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGA                                                                   >  SRR4302547.2318237/1‑94 (MQ=60)
                      GCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302547.3508824/1‑94 (MQ=60)
                      CCCGGCCGACGAATCCAACAGGCGATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302547.2985418/1‑94 (MQ=60)
                      CCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGAT                                                                  >  SRR4302547.1992781/1‑94 (MQ=60)
                       CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATT                                                                 <  SRR4302547.1256339/94‑1 (MQ=60)
                       CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATT                                                                 >  SRR4302547.113294/1‑94 (MQ=60)
                           CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             <  SRR4302547.4564114/94‑1 (MQ=60)
                            CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302547.631957/1‑94 (MQ=60)
                            CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            <  SRR4302547.4953212/94‑1 (MQ=60)
                             GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302547.5029135/1‑94 (MQ=60)
                             GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302547.3799866/1‑94 (MQ=60)
                             GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302547.4838286/94‑1 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          <  SRR4302547.2506168/94‑1 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          >  SRR4302547.4408224/1‑94 (MQ=60)
                              ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGCTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          >  SRR4302547.6359655/1‑94 (MQ=60)
                               CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                         >  SRR4302547.6112127/1‑94 (MQ=60)
                                GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                        >  SRR4302547.1593248/1‑94 (MQ=60)
                                GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                        >  SRR4302547.873458/1‑94 (MQ=60)
                                 AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCTCTGGTGATTGATAACGCTT                                                       <  SRR4302547.3179160/94‑1 (MQ=60)
                                  ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTA                                                      >  SRR4302547.2995488/1‑94 (MQ=60)
                                   TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302547.3724977/94‑1 (MQ=60)
                                    CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302547.558039/94‑1 (MQ=60)
                                     CAACAGGCAATGTGATTGCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATATCGCTTATGG                                                   <  SRR4302547.6599821/94‑1 (MQ=60)
                                     CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302547.2883363/1‑94 (MQ=60)
                                     CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302547.2827311/1‑94 (MQ=60)
                                      AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGT                                                  >  SRR4302547.4541523/1‑94 (MQ=60)
                                      AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  >  SRR4302547.6067480/1‑94 (MQ=60)
                                       ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 <  SRR4302547.4803865/94‑1 (MQ=60)
                                         AGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTC                                               >  SRR4302547.3202036/1‑94 (MQ=60)
                                          GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              >  SRR4302547.2779508/1‑94 (MQ=60)
                                          GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              <  SRR4302547.2406578/94‑1 (MQ=60)
                                           GCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                             <  SRR4302547.2368276/94‑1 (MQ=60)
                                           GCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                             <  SRR4302547.1160143/94‑1 (MQ=60)
                                             AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           >  SRR4302547.5141851/1‑94 (MQ=60)
                                             AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           >  SRR4302547.6477788/1‑94 (MQ=60)
                                             AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           <  SRR4302547.5527861/94‑1 (MQ=60)
                                                GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGGATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302547.6065923/1‑94 (MQ=60)
                                                GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302547.5014947/1‑94 (MQ=60)
                                                GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302547.104998/1‑94 (MQ=60)
                                                GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302547.4274279/1‑94 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302547.717956/94‑1 (MQ=60)
                                                 TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302547.4063153/1‑94 (MQ=60)
                                                  GATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCG                                      >  SRR4302547.890057/1‑94 (MQ=60)
                                                    TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    >  SRR4302547.3444319/1‑94 (MQ=60)
                                                     TACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGT                                   <  SRR4302547.3535345/94‑1 (MQ=60)
                                                      ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  <  SRR4302547.4595460/94‑1 (MQ=60)
                                                      ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  <  SRR4302547.3072792/94‑1 (MQ=60)
                                                       CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302547.2167216/1‑94 (MQ=60)
                                                       CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302547.6129799/1‑94 (MQ=60)
                                                       CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302547.2034197/94‑1 (MQ=60)
                                                       CTGACGAAGAGGTGCTGAAGCTTGACGCGCTGGCC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302547.4857641/94‑1 (MQ=60)
                                                         GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               >  SRR4302547.2204604/1‑94 (MQ=60)
                                                         GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               >  SRR4302547.2212951/1‑94 (MQ=60)
                                                         GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               >  SRR4302547.3630497/1‑94 (MQ=60)
                                                           CGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGGTTGATAAAGCTTATtgggtccccctccccgggatcatc                             >  SRR4302547.5844967/1‑70 (MQ=60)
                                                           CGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                             >  SRR4302547.1195541/1‑94 (MQ=60)
                                                            GAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                            <  SRR4302547.1894547/94‑1 (MQ=60)
                                                               GAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                         >  SRR4302547.5767816/1‑94 (MQ=60)
                                                                AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        >  SRR4302547.556100/1‑94 (MQ=60)
                                                                 GTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGT                       <  SRR4302547.1065544/94‑1 (MQ=60)
                                                                   TGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                     >  SRR4302547.5028017/1‑94 (MQ=60)
                                                                    GCTGAAGCTTGACGCGCTGGCT‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    <  SRR4302547.1982988/94‑1 (MQ=60)
                                                                    GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    >  SRR4302547.1988276/1‑94 (MQ=60)
                                                                    GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    <  SRR4302547.2733163/94‑1 (MQ=60)
                                                                    GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    >  SRR4302547.5103025/1‑94 (MQ=60)
                                                                      TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  <  SRR4302547.1800874/94‑1 (MQ=60)
                                                                       GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302547.5954040/94‑1 (MQ=60)
                                                                       GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302547.2091144/94‑1 (MQ=60)
                                                                        AAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC                <  SRR4302547.4551285/94‑1 (MQ=60)
                                                                         AGTTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               >  SRR4302547.4273678/1‑94 (MQ=60)
                                                                          GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              >  SRR4302547.4761807/1‑94 (MQ=60)
                                                                           CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGGATCATCTTCAGTGAAGCGCGCC             >  SRR4302547.2816283/1‑94 (MQ=60)
                                                                            TTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCC            <  SRR4302547.2696413/94‑1 (MQ=60)
                                                                             TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302547.3591087/1‑94 (MQ=60)
                                                                             TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302547.5971376/1‑94 (MQ=60)
                                                                             TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302547.5131090/1‑94 (MQ=60)
                                                                             TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302547.3799207/1‑94 (MQ=60)
                                                                              GACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC          >  SRR4302547.1545816/1‑94 (MQ=60)
                                                                               ACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCT         <  SRR4302547.3123973/94‑1 (MQ=60)
                                                                               ACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCT         <  SRR4302547.1849822/94‑1 (MQ=60)
                                                                                 GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302547.6349836/94‑1 (MQ=60)
                                                                                 GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302547.2856509/94‑1 (MQ=60)
                                                                                  CGCTGGCG‑AATCAACACGCCATTCCGCTGGTGATTGATAACGCTTATGGCGCCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG      <  SRR4302547.3219507/94‑1 (MQ=60)
                                                                                   GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302547.5400429/94‑1 (MQ=60)
                                                                                   GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302547.382885/94‑1 (MQ=60)
                                                                                   GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     >  SRR4302547.1376236/1‑94 (MQ=60)
                                                                                    CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    <  SRR4302547.920554/94‑1 (MQ=60)
                                                                                       GGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  SRR4302547.846101/1‑94 (MQ=60)
                                                                                        |                                                                                            
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737868‑3738047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑