Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,765,203 A→T Q119L (CAG→CTG)  yibJ → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,765,2030AT98.0% 165.9 / NA 50Q119L (CAG→CTG) yibJhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (25/24);  minor base G (0/1);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292
                   |                                                                                         
cGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCg                  >  2:5728399/1‑93 (MQ=9)
 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 <  2:2406783/93‑1 (MQ=12)
 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 >  1:1015056/1‑93 (MQ=12)
 gCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCgg                 >  2:1551705/1‑93 (MQ=12)
  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                <  2:3226363/93‑1 (MQ=16)
  cTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGt                >  2:4116259/1‑93 (MQ=255)
   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  2:4198454/1‑93 (MQ=255)
   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               <  2:2672141/93‑1 (MQ=255)
   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:826424/1‑93 (MQ=255)
   tattatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTc               >  1:2253177/1‑93 (MQ=255)
    attatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGTGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCa              <  2:3825174/93‑1 (MQ=11)
     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             <  2:307340/93‑1 (MQ=255)
     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  2:1877967/1‑93 (MQ=255)
     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             >  1:2352516/1‑93 (MQ=255)
     ttatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAt             <  1:5554556/93‑1 (MQ=255)
      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:1577886/1‑93 (MQ=255)
      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            <  2:313104/93‑1 (MQ=255)
      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:3425773/1‑93 (MQ=255)
      tatGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATa            >  1:1577887/1‑93 (MQ=255)
       atGGCCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  2:1847053/93‑1 (MQ=255)
       atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  1:1932376/93‑1 (MQ=255)
       atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           >  2:1493085/1‑93 (MQ=255)
       atGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaa           <  2:456886/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:2258871/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  1:1822487/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  2:5816045/93‑1 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          >  1:3909788/1‑93 (MQ=255)
        tGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATaaa          <  1:376328/93‑1 (MQ=255)
         gACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         >  1:1611827/1‑93 (MQ=255)
         gACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAt         >  2:209237/1‑93 (MQ=255)
          aCCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATg        <  2:4110017/93‑1 (MQ=255)
           cccGCTGCTGGGTCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       <  2:2876585/93‑1 (MQ=255)
           cccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGt       >  1:3268822/1‑93 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:2779498/1‑93 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:3090721/1‑93 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:3083752/1‑93 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  2:3156771/93‑1 (MQ=255)
            ccGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      >  1:3054957/1‑93 (MQ=255)
            ccGCTGCGGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTa      <  1:3553604/93‑1 (MQ=255)
             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  2:1742578/93‑1 (MQ=255)
             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:779696/93‑1 (MQ=255)
             cGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAg     <  1:4321246/93‑1 (MQ=255)
              gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  2:306766/93‑1 (MQ=255)
              gctgctGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGa    <  1:5252966/93‑1 (MQ=255)
               ctgctgGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAt   <  2:4148134/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:389063/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  1:5345533/1‑93 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:4405198/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  2:4496857/1‑93 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  1:4635593/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:4797144/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  1:1715137/1‑93 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  <  2:6101815/93‑1 (MQ=255)
                tGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATc  >  2:736231/1‑93 (MQ=255)
                   |                                                                                         
CGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC  >  NC_000913/3765184‑3765292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCA  >  NC_000913/3765110‑3765296
                                                                                             |                                                                                             
ATCATCTGCAGCAGTTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCA                                                                                               <  SRR4302532.6203759/94‑1 (MQ=0)
ATCATCTGCAGCAGCTCATTCGCCTGCCTGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCT                                                                                               <  SRR4302532.2057841/94‑1 (MQ=0)
ATCATCTGCAGCAGCTCATTCGCCTGCCGGGTCAGCAGTATGGTGAGGGGTCCGGCCTGTATTACAACCGCCGCCGCTATTATGACCCGCTGCT                                                                                               >  SRR4302532.1714548/1‑94 (MQ=0)
ATCATCTGCAGCAGCTCATTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCT                                                                                               <  SRR4302532.6302795/94‑1 (MQ=0)
ATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCA                                                                                               <  SRR4302532.3890147/94‑1 (MQ=0)
   AGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGG                                                                                            <  SRR4302532.913978/94‑1 (MQ=0)
    GCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGG                                                                                           <  SRR4302532.1757407/94‑1 (MQ=0)
    GCTGCAGCAGCTTATCCGCCTGCAGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGG                                                                                           <  SRR4302532.3621667/94‑1 (MQ=0)
     CTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGG                                                                                          >  SRR4302532.1729610/1‑94 (MQ=0)
       GCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGCCCCGCTGCAGGGGCGG                                                                                        <  SRR4302532.889882/94‑1 (MQ=0)
       GCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGG                                                                                        >  SRR4302532.4415014/1‑94 (MQ=0)
       GCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGG                                                                                        <  SRR4302532.3703976/94‑1 (MQ=0)
         AGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTA                                                                                      >  SRR4302532.3684233/1‑94 (MQ=0)
          GCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTAT                                                                                     <  SRR4302532.2038396/94‑1 (MQ=0)
          GCAGCTCATTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTAT                                                                                     >  SRR4302532.684859/1‑94 (MQ=0)
            cgctctttCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACACCCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATAT                                                                                   <  SRR4302532.3985740/86‑1 (MQ=0)
            AGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATAT                                                                                   >  SRR4302532.3838677/1‑94 (MQ=0)
            AGCTCATGCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATAT                                                                                   <  SRR4302532.2074455/94‑1 (MQ=0)
             GCTCATTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATC                                                                                  <  SRR4302532.6119874/94‑1 (MQ=0)
             GCTCATTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATC                                                                                  >  SRR4302532.217535/1‑94 (MQ=0)
              cacattCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCA                                                                                 >  SRR4302532.1186725/7‑94 (MQ=0)
              CTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCA                                                                                 <  SRR4302532.3704481/94‑1 (MQ=0)
              CTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCA                                                                                 >  SRR4302532.3827481/1‑94 (MQ=0)
              CTCATTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCA                                                                                 <  SRR4302532.2982539/94‑1 (MQ=0)
              CTCATTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCA                                                                                 >  SRR4302532.2484561/1‑94 (MQ=0)
               tcattCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCAC                                                                                >  SRR4302532.5815299/6‑94 (MQ=0)
                 ATCCGCCTGCGGGGGCAGCAGTATGATCAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCGCTC                                                                              <  SRR4302532.1006487/94‑1 (MQ=0)
                  TTCGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCA                                                                             <  SRR4302532.2312831/94‑1 (MQ=0)
                  TCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCA                                                                             >  SRR4302532.416473/1‑94 (MQ=0)
                   CCGCCTGCCGGGTCAGCAGTATGATGAGGGGTCCGGCCTGTATTACAACCACCCCCGCTATTATGACCCGCTGCTGGGGCGGTATATCCCTCAG                                                                            <  SRR4302532.1262138/94‑1 (MQ=0)
                   CCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAG                                                                            >  SRR4302532.614138/1‑94 (MQ=0)
                    CGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGG                                                                           >  SRR4302532.4736798/1‑94 (MQ=0)
                    CGCCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGG                                                                           <  SRR4302532.4147888/94‑1 (MQ=0)
                     GCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGA                                                                          >  SRR4302532.1101154/1‑94 (MQ=0)
                      CCTGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGAT                                                                         <  SRR4302532.1764196/94‑1 (MQ=0)
                        TGCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCC                                                                       >  SRR4302532.4632028/1‑94 (MQ=0)
                        TGCCGGGTCAACAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCC                                                                       >  SRR4302532.1978869/1‑94 (MQ=0)
                         GCCGGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCG                                                                      <  SRR4302532.3579742/94‑1 (MQ=0)
                          CCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGA                                                                     >  SRR4302532.2211605/1‑94 (MQ=0)
                          CCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGA                                                                     <  SRR4302532.3850365/94‑1 (MQ=0)
                            gggtaAGCAGTATGATGAGGGGTCCGGACTGTATGACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATAGCACTCAGGACCCGATT                                                                   <  SRR4302532.4556485/89‑1 (MQ=0)
                            GGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATT                                                                   <  SRR4302532.5989515/94‑1 (MQ=0)
                            GGGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATT                                                                   <  SRR4302532.3349121/94‑1 (MQ=0)
                             GGTCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTG                                                                  <  SRR4302532.2889248/94‑1 (MQ=0)
                              GGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTAG                                                                 >  SRR4302532.5786037/1‑94 (MQ=0)
                               TCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGA                                                                <  SRR4302532.4833572/94‑1 (MQ=0)
                                 AGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCT                                                              <  SRR4302532.813439/94‑1 (MQ=0)
                                   CAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGA                                                            >  SRR4302532.2591914/1‑94 (MQ=0)
                                    AGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAA                                                           >  SRR4302532.4245947/1‑94 (MQ=0)
                                    AGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAA                                                           <  SRR4302532.5877325/94‑1 (MQ=0)
                                      TATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGG                                                         >  SRR4302532.1931568/1‑94 (MQ=0)
                                       ATGATGAGGAGTCCGGCCTGTATTACACCCGCCCCCGCTATTATGCCCCGCTGCAGGGGCGGTATATCCCTCAGGATCCGATTGGGCTGAAGGG                                                        <  SRR4302532.991118/94‑1 (MQ=0)
                                       ATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGG                                                        <  SRR4302532.5061452/94‑1 (MQ=0)
                                       ATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGG                                                        <  SRR4302532.4594227/94‑1 (MQ=0)
                                       ATGATGAGGAGTCCGACCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGACGGG                                                        <  SRR4302532.5683304/94‑1 (MQ=0)
                                        TGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGG                                                       >  SRR4302532.3678475/1‑94 (MQ=0)
                                         GATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGG                                                      <  SRR4302532.2172811/94‑1 (MQ=0)
                                         GATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCGGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGG                                                      <  SRR4302532.1188576/94‑1 (MQ=0)
                                          ATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGG                                                     >  SRR4302532.3200921/1‑94 (MQ=0)
                                          ATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGG                                                     >  SRR4302532.4467661/1‑94 (MQ=0)
                                          ATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGG                                                     <  SRR4302532.6181228/94‑1 (MQ=0)
                                           TGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGA                                                    >  SRR4302532.5033467/1‑94 (MQ=0)
                                           TGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGA                                                    <  SRR4302532.6004586/94‑1 (MQ=0)
                                            GAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGAG                                                   >  SRR4302532.6043754/1‑94 (MQ=0)
                                              GGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGG                                                 <  SRR4302532.5921963/94‑1 (MQ=0)
                                              GGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGG                                                 <  SRR4302532.3979565/94‑1 (MQ=0)
                                               GAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGA                                                <  SRR4302532.80146/94‑1 (MQ=0)
                                                AGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAT                                               >  SRR4302532.4940853/1‑94 (MQ=0)
                                                AGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAA                                               >  SRR4302532.1058378/1‑94 (MQ=0)
                                                 GTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAAT                                              >  SRR4302532.3681151/1‑94 (MQ=0)
                                                   CCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTT                                            <  SRR4302532.6091278/94‑1 (MQ=0)
                                                   CCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTT                                            >  SRR4302532.1855358/1‑94 (MQ=0)
                                                    cgcaCTGTATTACAACCGCCGCCGCTATTATGACCCGGTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTT                                           <  SRR4302532.1090997/90‑1 (MQ=0)
                                                     GGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTT                                          <  SRR4302532.1030028/94‑1 (MQ=0)
                                                       CCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTAT                                        >  SRR4302532.3747683/1‑94 (MQ=0)
                                                       CCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTAT                                        >  SRR4302532.5000425/1‑94 (MQ=0)
                                                       CCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTAT                                        >  SRR4302532.5500199/1‑94 (MQ=0)
                                                        CTGTATTACACCCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                       <  SRR4302532.2923730/94‑1 (MQ=0)
                                                        CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                       >  SRR4302532.808140/1‑94 (MQ=0)
                                                        CTGTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATC                                       >  SRR4302532.1308164/1‑94 (MQ=0)
                                                         TGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCA                                      <  SRR4302532.1890517/94‑1 (MQ=0)
                                                          GTATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAG                                     <  SRR4302532.89412/94‑1 (MQ=0)
                                                           TATTACACCCGCCACCGCTATTATGAGCCGCTGCTGGGGCGGTATATCACGCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGT                                    <  SRR4302532.2365062/94‑1 (MQ=0)
                                                            ATTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTA                                   <  SRR4302532.23260/94‑1 (MQ=0)
                                                            ATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTA                                   >  SRR4302532.3302086/1‑94 (MQ=0)
                                                            ATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTA                                   >  SRR4302532.3473962/1‑94 (MQ=0)
                                                             TTACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTAT                                  <  SRR4302532.424044/94‑1 (MQ=0)
                                                             TTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTAT                                  <  SRR4302532.1471810/94‑1 (MQ=0)
                                                              TACAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATC                                 <  SRR4302532.13680/94‑1 (MQ=0)
                                                                CAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCG                               >  SRR4302532.5780058/1‑94 (MQ=0)
                                                                CAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCG                               <  SRR4302532.3066879/94‑1 (MQ=0)
                                                                CAACCGCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGAATCCG                               >  SRR4302532.4747117/1‑94 (MQ=0)
                                                                 AACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGT                              >  SRR4302532.4637012/1‑94 (MQ=0)
                                                                 AACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGT                              >  SRR4302532.5032814/1‑94 (MQ=0)
                                                                    CGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGA                           >  SRR4302532.5656267/1‑94 (MQ=0)
                                                                     GCCACCGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAA                          <  SRR4302532.1099026/94‑1 (MQ=0)
                                                                      CCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAAT                         <  SRR4302532.3383766/94‑1 (MQ=0)
                                                                      CCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAAT                         >  SRR4302532.4482994/1‑94 (MQ=0)
                                                                        CCCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCC                       <  SRR4302532.803457/94‑1 (MQ=0)
                                                                         CCACTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCG                      <  SRR4302532.1456694/94‑1 (MQ=0)
                                                                          CGCTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGG                     >  SRR4302532.1049521/1‑94 (MQ=2)
                                                                           GCTATTATGACCCGCTGCAGGGGAGGTATATCACTCAGGATCCGATTGGGCTGGAGGGGGGATGGAGTTTTTATCAGTATCCGTTGAATCAGGT                    >  SRR4302532.3714296/1‑94 (MQ=0)
                                                                            CTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTC                   >  SRR4302532.854694/1‑94 (MQ=7)
                                                                            CTATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTC                   >  SRR4302532.2327968/1‑94 (MQ=7)
                                                                              ATTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCAT                 >  SRR4302532.2431011/1‑94 (MQ=11)
                                                                               TTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATA                <  SRR4302532.5747390/94‑1 (MQ=14)
                                                                               TTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATA                >  SRR4302532.3540544/1‑94 (MQ=14)
                                                                               TTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATA                >  SRR4302532.1631308/1‑94 (MQ=14)
                                                                               TTATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATA                >  SRR4302532.1631309/1‑94 (MQ=14)
                                                                                 ATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAA              <  SRR4302532.1996684/94‑1 (MQ=22)
                                                                                 ATGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAA              >  SRR4302532.4041614/1‑94 (MQ=22)
                                                                                  TGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAT             >  SRR4302532.1666379/1‑94 (MQ=24)
                                                                                  TGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAT             <  SRR4302532.1883197/94‑1 (MQ=24)
                                                                                  TGACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAAT             <  SRR4302532.389400/94‑1 (MQ=24)
                                                                                    ACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGTGGTTGGAATTTTTATCAGTATCGGTTGACTCCGGTCATAAATGA           >  SRR4302532.3377531/1‑94 (MQ=20)
                                                                                    ACCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGT           >  SRR4302532.3377532/1‑94 (MQ=29)
                                                                                     CCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA          >  SRR4302532.3193138/1‑94 (MQ=31)
                                                                                     CCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA          >  SRR4302532.3185762/1‑94 (MQ=31)
                                                                                     CCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA          >  SRR4302532.3155819/1‑94 (MQ=31)
                                                                                     CCCGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTA          >  SRR4302532.2872748/1‑94 (MQ=31)
                                                                                      CCGCTGCGGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAG         <  SRR4302532.3672504/94‑1 (MQ=34)
                                                                                       CGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGA        <  SRR4302532.806520/94‑1 (MQ=36)
                                                                                       CGCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGA        <  SRR4302532.4467286/94‑1 (MQ=36)
                                                                                        GCTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGAT       <  SRR4302532.5434060/94‑1 (MQ=39)
                                                                                         CTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC      >  SRR4302532.5530990/1‑94 (MQ=41)
                                                                                         CTGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATC      >  SRR4302532.1772952/1‑94 (MQ=41)
                                                                                          TGCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCC     <  SRR4302532.4793648/94‑1 (MQ=44)
                                                                                          TGCTGGGGCGGAATATCACTCAAGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATATATGTAGATCC     >  SRR4302532.4445223/1‑94 (MQ=28)
                                                                                           GCTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCG    <  SRR4302532.832844/94‑1 (MQ=47)
                                                                                           GCTGGGGCGGGATATCACTCAGGATCCGATTGGACTGAAGGGGGGTTGGAATTTTTATCAGTATCCGTTGAATCCGGGCATAAATGTAGTTCCG    >  SRR4302532.5419283/1‑94 (MQ=18)
                                                                                            CTGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGC   >  SRR4302532.2341084/1‑94 (MQ=49)
                                                                                             TGGGGCGGTATATCACTCAGGATCCGATTGGACTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGGAGATCCGCA  >  SRR4302532.3585193/1‑94 (MQ=38)
                                                                                             |                                                                                             
ATCAGCTGCAGCAGCTTATCCGCCTGCCGGGGCAGCAGTATGATGAGGAGTCCGGCCTGTATTACAACCGCCACCGCTATTATGACCCGCTGCAGGGGCGGTATATCACTCAGGATCCGATTGGGCTGAAGGGGGGATGGAATTTTTATCAGTATCCGTTGAATCCGGTCATAAATGTAGATCCGCA  >  NC_000913/3765110‑3765296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑