Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,872,4501.G99.6% 935.5 / ‑4.2 259coding (338/387 nt)yidWregulator protein for dgo operon
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (143/115);  total (143/116)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536
                                                                                              |                                                                                      
tCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑t                                                                                         <  2:2692331/93‑1 (MQ=255)
 cGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tg                                                                                        >  1:3018303/1‑92 (MQ=255)
 cGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tg                                                                                        >  2:1687170/1‑92 (MQ=255)
  gTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tgc                                                                                       >  2:2891607/1‑91 (MQ=255)
   ttAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gcc                                                                                     >  2:5773987/1‑93 (MQ=255)
    tAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccc                                                                                    <  2:3701462/93‑2 (MQ=255)
     aaaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccca                                                                                   >  2:3184763/1‑91 (MQ=255)
      aaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCaa                                                                                   >  1:868405/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCGGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAAc                                                                                  >  2:1200302/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAAc                                                                                  <  1:542225/93‑1 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACg                                                                                 >  2:3875899/1‑93 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACg                                                                                 <  1:1340900/93‑1 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACgg                                                                                <  2:6069150/93‑1 (MQ=255)
          cTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACggg                                                                               >  2:1124135/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGc                                                                              >  2:1596236/1‑93 (MQ=255)
             cgcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCaa                                                                            <  1:4898769/93‑1 (MQ=255)
              gcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAAt                                                                           <  1:4379610/93‑1 (MQ=255)
                ggTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCg                                                                         >  2:115599/1‑93 (MQ=255)
                ggTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCg                                                                         >  1:1988196/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGc                                                                        >  2:2046297/1‑93 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCATCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     <  1:127315/93‑1 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     <  2:492975/93‑1 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     <  1:4761563/93‑1 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     >  1:2519082/1‑93 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCgg                                                                     >  2:3256285/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGt                                                                    >  1:2847703/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGt                                                                    >  2:2595539/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGt                                                                    <  1:2645746/93‑1 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGt                                                                    >  1:3922075/1‑93 (MQ=255)
                      gATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGCCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGtt                                                                   <  2:142881/93‑1 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTcc                                                                 >  2:2374296/1‑93 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTcc                                                                 >  2:4555549/1‑93 (MQ=255)
                          gttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTccac                                                               >  1:2744846/1‑93 (MQ=255)
                          gttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTccac                                                               <  1:5899976/93‑1 (MQ=255)
                            tgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTccacca                                                             >  2:5403623/1‑93 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           >  1:4112355/1‑93 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           <  2:3290402/93‑1 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAt                                                          >  1:6121778/1‑93 (MQ=255)
                                aaTCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAtt                                                         >  1:644255/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGCttt                                                        >  2:901523/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  1:3507872/1‑93 (MQ=255)
                                   catcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCg                                                      <  1:3971423/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    <  2:3773650/93‑1 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAAGTCGCa                                                    >  2:5466561/1‑93 (MQ=255)
                                      ctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  2:5954932/93‑1 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 >  2:4320921/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               <  2:1986927/93‑1 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCAAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCCCCAGATTTCGCACTTCg                                               <  1:2285149/93‑1 (MQ=255)
                                            cAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCt                                             <  2:5191207/93‑1 (MQ=255)
                                             aGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTc                                            <  1:5974901/93‑1 (MQ=255)
                                              gcgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCa                                           <  1:2767576/93‑1 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         <  2:4429249/93‑1 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         >  2:3915174/1‑93 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         <  1:4496293/93‑1 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGAGCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         <  2:1587600/93‑1 (MQ=255)
                                                 ccGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATgg                                        <  2:3226615/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       <  1:21827/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       >  1:4632315/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  1:3536758/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  2:688624/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  2:180990/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  1:3846583/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    <  1:5838119/93‑1 (MQ=255)
                                                         aTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCTGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATa                                <  1:5665827/93‑1 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               >  2:4644339/1‑93 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               <  1:3296490/93‑1 (MQ=255)
                                                           cTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAg                              <  2:3492082/93‑1 (MQ=255)
                                                           cTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAg                              >  1:515857/1‑93 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:4006134/93‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:5232722/93‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            <  1:5757006/93‑1 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          >  2:2817691/1‑93 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          >  1:3151290/1‑93 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          <  2:4822994/93‑1 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          >  2:4380273/1‑93 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         <  2:2483785/93‑1 (MQ=255)
                                                                 cAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAATGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTgg                        <  2:5029258/93‑1 (MQ=255)
                                                                 cAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTgg                        <  1:4296491/93‑1 (MQ=255)
                                                                 cAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTgg                        <  2:3650636/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       >  2:1279844/1‑93 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  1:2725250/93‑1 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  1:2040326/1‑93 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  1:1030087/1‑93 (MQ=255)
                                                                     tCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCg                    <  1:1143126/93‑1 (MQ=255)
                                                                     tCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCg                    >  2:1074218/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   <  2:1329445/93‑1 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   <  1:1481622/93‑1 (MQ=255)
                                                                        cTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAg                 >  2:778098/1‑93 (MQ=255)
                                                                          ggAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTc               >  2:1766116/1‑93 (MQ=255)
                                                                          ggAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTc               >  2:6051238/1‑93 (MQ=255)
                                                                           gAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCa              <  1:1763117/93‑1 (MQ=255)
                                                                             aGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  1:5806235/93‑1 (MQ=255)
                                                                                cgcgcgCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTc         <  1:1679138/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        >  2:4449066/1‑93 (MQ=255)
                                                                                  cgcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCa       >  1:4206576/1‑93 (MQ=255)
                                                                                  cgcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCa       <  2:5691234/93‑1 (MQ=255)
                                                                                  cgcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGGCGTAGTCATTTTCCa       >  2:4967807/1‑93 (MQ=255)
                                                                                   gcgcTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCat      <  1:2362611/93‑2 (MQ=255)
                                                                                      ctctGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAc   >  2:2113311/1‑93 (MQ=255)
                                                                                       tctGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  >  1:819684/1‑93 (MQ=255)
                                                                                              |                                                                                      
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  >  NC_000913/3872358‑3872543
                                                                                             |                                                                                              
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑G                                                                                              >  SRR4302532.3117886/1‑94 (MQ=60)
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑G                                                                                              >  SRR4302532.3137717/1‑94 (MQ=60)
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑G                                                                                              <  SRR4302532.3376330/94‑1 (MQ=60)
 CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑GC                                                                                             >  SRR4302532.4366952/1‑94 (MQ=60)
  GTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCCGCGCCGATTCAATCTGCGCCAGATAGCTGGAAGTCGCGCGCTCTGC‑T‑GCC                                                                                            >  SRR4302532.1360319/1‑94 (MQ=60)
  GTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑GCC                                                                                            <  SRR4302532.5725271/94‑1 (MQ=60)
   TTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCC                                                                                            >  SRR4302532.2847364/1‑94 (MQ=60)
    TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA                                                                                           >  SRR4302532.1700815/1‑94 (MQ=60)
     AAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAA                                                                                          >  SRR4302532.898162/1‑94 (MQ=60)
       ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACG                                                                                        <  SRR4302532.561109/94‑1 (MQ=60)
        CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGG                                                                                       <  SRR4302532.1385743/94‑1 (MQ=60)
         GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGG                                                                                      <  SRR4302532.662851/94‑1 (MQ=60)
         CCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGG                                                                                      <  SRR4302532.1942423/94‑1 (MQ=60)
          CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGC                                                                                     >  SRR4302532.2658422/1‑94 (MQ=60)
          CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGC                                                                                     >  SRR4302532.3639212/1‑94 (MQ=60)
          CTTCGCGGTCCTGATTGTTGGCAATCATATCGTTAAGCGCCGATTAAATCTGCGTCAGATAGCTGGAAGTTGCGCGCTCTGC‑TGCCCAACAGGC                                                                                     >  SRR4302532.2928920/1‑94 (MQ=60)
            TCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAA                                                                                   <  SRR4302532.293324/94‑1 (MQ=60)
             CGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAAT                                                                                  <  SRR4302532.5067126/94‑1 (MQ=60)
              GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATC                                                                                 <  SRR4302532.4528349/94‑1 (MQ=60)
               CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCG                                                                                >  SRR4302532.2054270/1‑94 (MQ=60)
                GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTAGCGCGCTCTGC‑TGCCCAACGGGCAATCGC                                                                               >  SRR4302532.2243600/1‑94 (MQ=60)
                 GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCC                                                                              <  SRR4302532.5105995/94‑1 (MQ=60)
                 GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCC                                                                              <  SRR4302532.2477582/94‑1 (MQ=60)
                 GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCC                                                                              >  SRR4302532.4530814/1‑94 (MQ=60)
                  TCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCG                                                                             >  SRR4302532.6057152/1‑94 (MQ=60)
                   TCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302532.3786595/1‑94 (MQ=60)
                   CCTGATTGTTTGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGG                                                                            <  SRR4302532.4963703/94‑1 (MQ=60)
                   CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302532.5908632/1‑94 (MQ=60)
                   CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302532.4888227/1‑94 (MQ=60)
                   CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302532.2603131/1‑94 (MQ=60)
                   CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGG                                                                            >  SRR4302532.242394/1‑94 (MQ=60)
                    CTGATTGTTGGCAATCATCTCGTTCATCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGT                                                                           <  SRR4302532.131612/94‑1 (MQ=60)
                    CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGT                                                                           <  SRR4302532.4924596/94‑1 (MQ=60)
                    CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302532.2942896/1‑94 (MQ=60)
                    CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302532.4054209/1‑94 (MQ=60)
                    CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302532.1876160/1‑94 (MQ=60)
                    CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGT                                                                           >  SRR4302532.877612/1‑94 (MQ=60)
                     TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTT                                                                          <  SRR4302532.2734469/94‑1 (MQ=60)
                     TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTT                                                                          >  SRR4302532.1598468/1‑94 (MQ=60)
                     TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTT                                                                          >  SRR4302532.1522613/1‑94 (MQ=60)
                      GATTGTCGGCAACCATCTCGTTCAGCGCCGATTAAATCTGCGCGAGATCGCTGGAAGCCGCGTGCTCTGC‑TCCCCAACGGGCAATaacaggttc                                                                         <  SRR4302532.2645615/94‑10 (MQ=53)
                       ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGGCGGTTCC                                                                        >  SRR4302532.1302600/1‑94 (MQ=60)
                        TTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCC                                                                       >  SRR4302532.2768904/1‑94 (MQ=60)
                         TGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCAC                                                                      >  SRR4302532.2837043/1‑94 (MQ=60)
                          GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACC                                                                     >  SRR4302532.1435092/1‑94 (MQ=60)
                          GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACC                                                                     <  SRR4302532.6104284/94‑1 (MQ=60)
                          GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACC                                                                     <  SRR4302532.3586791/94‑1 (MQ=60)
                           TTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCA                                                                    >  SRR4302532.2394716/1‑94 (MQ=60)
                             GGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGA                                                                  >  SRR4302532.4250399/1‑94 (MQ=60)
                              GCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAT                                                                 >  SRR4302532.5468218/1‑94 (MQ=60)
                              GCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGAT                                                                 >  SRR4302532.6333039/1‑94 (MQ=60)
                               CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                >  SRR4302532.666635/1‑94 (MQ=60)
                               CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                <  SRR4302532.6340092/94‑1 (MQ=60)
                               CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                >  SRR4302532.2293544/1‑94 (MQ=60)
                               CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                                <  SRR4302532.5655227/94‑1 (MQ=60)
                                AATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                               >  SRR4302532.3625363/1‑94 (MQ=60)
                                 ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                              <  SRR4302532.5031558/94‑1 (MQ=60)
                                 ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                              >  SRR4302532.94386/1‑94 (MQ=60)
                                  TCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCG                                                             <  SRR4302532.5461021/94‑1 (MQ=60)
                                   CATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGC                                                            <  SRR4302532.4105205/94‑1 (MQ=60)
                                    ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                           >  SRR4302532.5980895/1‑94 (MQ=60)
                                    ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                           >  SRR4302532.4084903/1‑94 (MQ=60)
                                      CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAcaattttcact                                                         >  SRR4302532.5412381/1‑83 (MQ=60)
                                        CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                       >  SRR4302532.3431683/1‑94 (MQ=60)
                                         GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      >  SRR4302532.3938185/1‑94 (MQ=60)
                                         GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      >  SRR4302532.2831479/1‑94 (MQ=60)
                                         GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                      >  SRR4302532.4282633/1‑94 (MQ=60)
                                          TTCAGCGCCGATTCAATCTGCGCAAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCCCCAGATTTCGCACTTCGC                                                     <  SRR4302532.2360999/94‑1 (MQ=60)
                                             AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                                  <  SRR4302532.6181434/94‑1 (MQ=60)
                                             AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                                  >  SRR4302532.706946/1‑94 (MQ=60)
                                              GCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAT                                                 <  SRR4302532.2860503/94‑1 (MQ=60)
                                               CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                >  SRR4302532.4648235/1‑94 (MQ=60)
                                               CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                                >  SRR4302532.5902534/1‑94 (MQ=60)
                                                GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                               <  SRR4302532.4250009/94‑1 (MQ=60)
                                                GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                               <  SRR4302532.4649789/94‑1 (MQ=60)
                                                 CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                              >  SRR4302532.2741862/1‑94 (MQ=60)
                                                 CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                              >  SRR4302532.4790263/1‑94 (MQ=60)
                                                  CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             >  SRR4302532.3655094/1‑94 (MQ=60)
                                                  CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             <  SRR4302532.22699/94‑1 (MQ=60)
                                                  CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             <  SRR4302532.2338758/94‑1 (MQ=60)
                                                  CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             >  SRR4302532.1152533/1‑94 (MQ=60)
                                                  CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             <  SRR4302532.22698/94‑1 (MQ=60)
                                                  CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                             <  SRR4302532.2823594/94‑1 (MQ=60)
                                                   GATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAC                                            >  SRR4302532.3976462/1‑94 (MQ=60)
                                                     TTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTG                                          <  SRR4302532.4273801/94‑1 (MQ=60)
                                                     TTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTG                                          <  SRR4302532.6040749/94‑1 (MQ=60)
                                                       CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                        >  SRR4302532.6174593/1‑94 (MQ=60)
                                                        AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                       >  SRR4302532.4757320/1‑94 (MQ=60)
                                                        AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                       >  SRR4302532.4059320/1‑94 (MQ=60)
                                                         ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCTGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                      <  SRR4302532.5863323/94‑1 (MQ=60)
                                                         ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                      <  SRR4302532.3645284/94‑1 (MQ=60)
                                                          TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                     <  SRR4302532.3406181/94‑1 (MQ=60)
                                                          TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                     >  SRR4302532.533862/1‑94 (MQ=60)
                                                          TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                     <  SRR4302532.6022535/94‑1 (MQ=60)
                                                            TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   >  SRR4302532.4503849/1‑94 (MQ=60)
                                                            TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   <  SRR4302532.302798/94‑1 (MQ=60)
                                                            TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   >  SRR4302532.6041655/1‑94 (MQ=60)
                                                            TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                                   <  SRR4302532.1294706/94‑1 (MQ=60)
                                                             GCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                                  <  SRR4302532.5957388/94‑1 (MQ=60)
                                                              CGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                                 >  SRR4302532.4175351/1‑94 (MQ=60)
                                                              CGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                                 >  SRR4302532.3255857/1‑94 (MQ=60)
                                                               GCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                                >  SRR4302532.2999037/1‑94 (MQ=60)
                                                                CCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                               >  SRR4302532.6152050/1‑94 (MQ=60)
                                                                 CAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                              <  SRR4302532.4441615/94‑1 (MQ=60)
                                                                  AGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                             <  SRR4302532.2816909/94‑1 (MQ=60)
                                                                  AGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                             >  SRR4302532.4852004/1‑94 (MQ=60)
                                                                  AGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                             <  SRR4302532.2671140/94‑1 (MQ=60)
                                                                  AGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                             >  SRR4302532.626780/1‑94 (MQ=60)
                                                                  AGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                             >  SRR4302532.626781/1‑94 (MQ=60)
                                                                   GATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302532.2108290/1‑94 (MQ=60)
                                                                   GATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            <  SRR4302532.1787210/94‑1 (MQ=60)
                                                                   GATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302532.1064892/1‑94 (MQ=60)
                                                                   GATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                            >  SRR4302532.3290146/1‑94 (MQ=60)
                                                                    ATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGACAATCGGCTGTTCCACCAGATTTCGCACTTCGCTCATGGCATTGATAAGCCGTGGGTCG                           <  SRR4302532.3989787/94‑1 (MQ=60)
                                                                     TCGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGT                          <  SRR4302532.1181261/94‑1 (MQ=60)
                                                                      CGCTGGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                         <  SRR4302532.1531557/94‑1 (MQ=60)
                                                                         TGGAAATCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                      >  SRR4302532.5244872/1‑94 (MQ=60)
                                                                          GGAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                     >  SRR4302532.2882432/1‑94 (MQ=60)
                                                                           GAAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAT                    <  SRR4302532.1822237/94‑1 (MQ=60)
                                                                            AAGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATT                   >  SRR4302532.445638/1‑94 (MQ=60)
                                                                             AGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT                  <  SRR4302532.6007979/94‑1 (MQ=60)
                                                                             AGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT                  <  SRR4302532.3178355/94‑1 (MQ=60)
                                                                             AGTCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT                  >  SRR4302532.1346523/1‑94 (MQ=60)
                                                                              GTCGCGCGCTTTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTT                 <  SRR4302532.4363353/94‑1 (MQ=60)
                                                                               TCGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTC                <  SRR4302532.77186/94‑1 (MQ=60)
                                                                                CGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC               <  SRR4302532.1735903/94‑1 (MQ=60)
                                                                                CGCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC               <  SRR4302532.869653/94‑1 (MQ=60)
                                                                                 GCGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCA              >  SRR4302532.4347782/1‑94 (MQ=60)
                                                                                  CGCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAG             <  SRR4302532.1820236/94‑1 (MQ=60)
                                                                                   GCGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCATC            <  SRR4302532.2441417/94‑1 (MQ=60)
                                                                                    CGCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA           >  SRR4302532.2103130/1‑94 (MQ=60)
                                                                                     GCTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC          >  SRR4302532.5959627/1‑94 (MQ=60)
                                                                                      CTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC         >  SRR4302532.847655/1‑94 (MQ=60)
                                                                                      CTCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC         >  SRR4302532.2598222/1‑94 (MQ=60)
                                                                                       TCTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC        >  SRR4302532.2903936/1‑94 (MQ=60)
                                                                                        CTGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCTGCACCCA       >  SRR4302532.2629213/1‑94 (MQ=60)
                                                                                         TGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT      <  SRR4302532.2111323/94‑1 (MQ=60)
                                                                                         TGC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT      <  SRR4302532.2202492/94‑1 (MQ=60)
                                                                                          GC‑TGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATT     <  SRR4302532.374668/94‑1 (MQ=60)
                                                                                             G‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  <  SRR4302532.286260/94‑1 (MQ=60)
                                                                                             G‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  <  SRR4302532.5253544/94‑1 (MQ=60)
                                                                                             G‑CCCAACGGGCAATCGCCGGTTCAACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  <  SRR4302532.3629273/94‑1 (MQ=60)
                                                                                             |                                                                                              
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCATTGCA  >  NC_000913/3872358‑3872543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑