Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,932,295 C→T L298F (CTC→TTC)  rbsA → ATP‑binding component of D‑ribose high‑affinity transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,932,2950CT99.6% 889.6 / ‑5.5 242L298F (CTC→TTC) rbsAATP‑binding component of D‑ribose high‑affinity transport system
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (131/110);  total (131/111)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 268 total aligned reads displayed.

GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAC  >  NC_000913/3932205‑3932384
                                                                                          |                                                                                         
gTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGttc                                                                                         >  2:4895532/1‑93 (MQ=255)
gTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGttc                                                                                         <  1:3073028/93‑1 (MQ=255)
 ttAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGttct                                                                                        >  2:2995822/1‑93 (MQ=255)
  tAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTa                                                                                       <  2:68005/93‑1 (MQ=255)
   aaCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTAc                                                                                      <  2:4748981/93‑1 (MQ=255)
   aaCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTAc                                                                                      <  2:4983056/93‑1 (MQ=255)
   aaCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTAc                                                                                      >  1:648586/1‑93 (MQ=255)
    aCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACg                                                                                     <  1:4916431/93‑1 (MQ=255)
    aCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACg                                                                                     <  2:2943265/93‑1 (MQ=255)
    aCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACg                                                                                     >  1:5896624/1‑93 (MQ=255)
      gATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgc                                                                                   <  2:5383956/93‑1 (MQ=255)
      gATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGGGTTCTACggg                                                                                   >  1:1753578/1‑92 (MQ=255)
       aTGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgcg                                                                                  >  1:2842322/1‑93 (MQ=255)
        tGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgcgc                                                                                 >  2:2049815/1‑93 (MQ=255)
          tctcTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCAc                                                                               >  1:5309413/1‑93 (MQ=255)
          tctcTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCAc                                                                               >  1:4145902/1‑93 (MQ=255)
           ctctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACt                                                                              >  2:4542223/1‑93 (MQ=255)
           ctctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACt                                                                              >  1:3361668/1‑93 (MQ=255)
            tctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTa                                                                             <  1:4827096/93‑1 (MQ=255)
             ctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAc                                                                            <  1:1458824/93‑1 (MQ=255)
             ctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAc                                                                            <  2:3069402/93‑1 (MQ=255)
              ttttACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAcc                                                                           >  1:5824064/1‑93 (MQ=255)
               tttactttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACcg                                                                          >  2:4227155/1‑93 (MQ=255)
                ttactttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACcgc                                                                         <  2:4561501/93‑1 (MQ=255)
                 tactttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACcgcg                                                                        >  2:3339000/1‑93 (MQ=255)
                 tactttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACcgcg                                                                        >  1:3521820/1‑93 (MQ=255)
                  actttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTGTACGGCGCACTAcccaga                                                                       >  2:1928834/1‑89 (MQ=255)
                  actttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCgcgc                                                                       >  2:5278474/1‑93 (MQ=255)
                     ttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCAcc                                                                    <  1:1137416/93‑1 (MQ=255)
                       acGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAg                                                                  <  2:2230766/93‑1 (MQ=255)
                        cGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGc                                                                 <  1:5968388/93‑1 (MQ=255)
                          cAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCgg                                                               >  1:2325680/1‑93 (MQ=255)
                           aaaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGt                                                              >  1:1837250/1‑93 (MQ=255)
                           aaaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGt                                                              >  2:5070981/1‑93 (MQ=255)
                            aaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGtt                                                             >  1:4448989/1‑93 (MQ=255)
                            aaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGtt                                                             >  2:153333/1‑93 (MQ=255)
                             aGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTa                                                            <  2:1305659/93‑1 (MQ=255)
                               gCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACg                                                          >  2:169307/1‑93 (MQ=255)
                                  aaaTTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCa                                                       >  1:871479/1‑93 (MQ=255)
                                    aTTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAcc                                                     >  1:2294916/1‑93 (MQ=255)
                                     ttCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAccc                                                    <  2:768109/93‑1 (MQ=255)
                                      tCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCt                                                   >  2:3752775/1‑93 (MQ=255)
                                       cTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTg                                                  >  2:1022705/1‑93 (MQ=255)
                                        ttGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTgg                                                 >  1:1564264/1‑93 (MQ=255)
                                        ttGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTgg                                                 >  2:2488581/1‑93 (MQ=255)
                                         tGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGATACATAACCCTGGa                                                >  1:1360899/1‑93 (MQ=255)
                                            cGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATgg                                             <  1:4329386/93‑1 (MQ=255)
                                             gTCTCTTGTGGGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTATGGCGCACTACCGCGCACCAGCGTTTTCGTCACCCTTTATggg                                            >  1:5497155/1‑93 (MQ=255)
                                             gTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATggg                                            <  1:5741923/93‑1 (MQ=255)
                                             gTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATggg                                            <  1:4712825/93‑1 (MQ=255)
                                             gTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATggg                                            >  2:112074/1‑93 (MQ=255)
                                               ctctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTATCGCGCACCAGCGGTTACGTCACCCTGGATGGGCa                                          >  2:3884326/1‑93 (MQ=255)
                                               ctctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCa                                          >  1:1981708/1‑93 (MQ=255)
                                               ctctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCa                                          <  2:5782435/93‑1 (MQ=255)
                                                tctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCAt                                         >  2:6137188/1‑93 (MQ=255)
                                                 ctGTTTTGCTGGGCGCAGGTCGTACCGATCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATg                                        <  1:608283/93‑1 (MQ=255)
                                                 ctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATg                                        >  1:3732798/1‑93 (MQ=255)
                                                  tGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGAGTTCTACGGCGCACTACCGCGCACCCGCGGTTACGTCAACCTGGATGGGCATGa                                       >  1:4467835/1‑93 (MQ=255)
                                                   ggTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGaa                                      <  1:293246/93‑1 (MQ=255)
                                                   ggTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGaa                                      <  2:801511/93‑1 (MQ=255)
                                                    gTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAg                                     <  2:2413542/93‑1 (MQ=255)
                                                    gTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAg                                     <  1:2220739/93‑1 (MQ=255)
                                                     tttGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGt                                    <  1:1025831/93‑1 (MQ=255)
                                                      ttGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTc                                   <  2:1460228/93‑1 (MQ=255)
                                                       tGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCg                                  <  1:3670161/93‑1 (MQ=255)
                                                        gATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGt                                 <  2:3670975/93‑1 (MQ=255)
                                                         aTGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGtt                                >  1:2053453/1‑93 (MQ=255)
                                                          tGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCCCCCTGGATGGGCATGAAGTCGTTa                               <  2:2682074/93‑1 (MQ=255)
                                                          tGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTa                               <  1:4630204/93‑1 (MQ=255)
                                                           gggCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAc                              <  2:5905401/93‑1 (MQ=255)
                                                            ggCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAcc                             <  1:4053556/93‑1 (MQ=255)
                                                            ggCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAcc                             <  1:2585223/93‑1 (MQ=255)
                                                             gcgcAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAccc                            <  2:5589872/93‑1 (MQ=255)
                                                               gcAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTCAGTCACCCTGGATGGGCATGAAGTCGTTACCCGt                          >  2:5956994/1‑93 (MQ=255)
                                                                cAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGtt                         <  2:2759149/93‑1 (MQ=255)
                                                                 cggTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTc                        <  1:5126420/92‑1 (MQ=255)
                                                                 aGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTc                        <  2:894143/93‑1 (MQ=255)
                                                                  ggTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCa                       >  1:1386751/1‑93 (MQ=255)
                                                                   gTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAc                      >  2:4019513/1‑93 (MQ=255)
                                                                   gTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAc                      >  1:814861/1‑93 (MQ=255)
                                                                    tCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAcc                     <  2:4596562/93‑1 (MQ=255)
                                                                    tCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAcc                     <  2:3451831/93‑1 (MQ=255)
                                                                     cGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCg                    <  2:4250449/93‑1 (MQ=255)
                                                                         ccGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAgg                >  1:1630409/1‑93 (MQ=255)
                                                                          cGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGa               <  2:1820297/93‑1 (MQ=255)
                                                                           gAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAt              <  2:5718757/93‑1 (MQ=255)
                                                                              cTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGc           <  1:285594/93‑1 (MQ=255)
                                                                              cTGATGAAAGTATTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGc           <  1:5574212/93‑1 (MQ=255)
                                                                               tgatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGcc          <  1:1690718/93‑1 (MQ=255)
                                                                                gatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCt         <  1:2680699/93‑1 (MQ=255)
                                                                                gatgaAAGTGTTCTACGGCGCACTACAGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCt         <  2:2561238/93‑1 (MQ=255)
                                                                                 atgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTg        >  2:3223871/1‑93 (MQ=255)
                                                                                 atgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTg        >  1:2428179/1‑93 (MQ=255)
                                                                                    aaaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCa     >  2:2433207/1‑93 (MQ=255)
                                                                                    aaaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCa     >  1:3126829/1‑93 (MQ=255)
                                                                                    aaaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCa     >  1:4717467/1‑93 (MQ=255)
                                                                                    aaaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCa     >  2:3393426/1‑93 (MQ=255)
                                                                                     aaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCaa    >  2:1392197/1‑93 (MQ=255)
                                                                                      aGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCaaa   >  1:3873122/1‑93 (MQ=255)
                                                                                       gtgtTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAc  <  2:722408/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAC  >  NC_000913/3932205‑3932384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGC  >  NC_000913/3932204‑3932387
                                                                                           |                                                                                            
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTC                                                                                            >  SRR4302532.60009/1‑94 (MQ=60)
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTC                                                                                            >  SRR4302532.4606635/1‑94 (MQ=60)
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGGTC                                                                                            >  SRR4302532.5661583/1‑94 (MQ=60)
 GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCT                                                                                           <  SRR4302532.3174557/94‑1 (MQ=60)
 GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCT                                                                                           <  SRR4302532.1682002/94‑1 (MQ=60)
 GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCT                                                                                           >  SRR4302532.2314913/1‑94 (MQ=60)
 GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCT                                                                                           <  SRR4302532.469160/94‑1 (MQ=60)
  TTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTA                                                                                          <  SRR4302532.3676703/94‑1 (MQ=60)
  TTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTA                                                                                          >  SRR4302532.5487628/1‑94 (MQ=60)
   TAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTAC                                                                                         >  SRR4302532.6244960/1‑94 (MQ=60)
   TAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTAC                                                                                         >  SRR4302532.671098/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                        >  SRR4302532.1823028/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                        >  SRR4302532.6100828/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                        >  SRR4302532.6100827/1‑94 (MQ=60)
     ACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGG                                                                                       >  SRR4302532.5570675/1‑94 (MQ=60)
     ACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGG                                                                                       >  SRR4302532.3852448/1‑94 (MQ=60)
     ACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGG                                                                                       <  SRR4302532.5085322/94‑1 (MQ=60)
      CGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGGGTTCTACGGG                                                                                      >  SRR4302532.1812472/1‑94 (MQ=60)
       GATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCG                                                                                     >  SRR4302532.2937325/1‑94 (MQ=60)
       GATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCG                                                                                     <  SRR4302532.899501/94‑1 (MQ=60)
        ATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGC                                                                                    >  SRR4302532.3044414/1‑94 (MQ=60)
         TGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCA                                                                                   <  SRR4302532.5288775/94‑1 (MQ=60)
         TGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCA                                                                                   >  SRR4302532.3044204/1‑94 (MQ=60)
         TGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCA                                                                                   >  SRR4302532.1592552/1‑94 (MQ=60)
          GTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCAC                                                                                  >  SRR4302532.5493009/1‑94 (MQ=60)
          GTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCAC                                                                                  >  SRR4302532.4285005/1‑94 (MQ=60)
           TCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACT                                                                                 >  SRR4302532.3473985/1‑94 (MQ=60)
            CTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGATTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTA                                                                                >  SRR4302532.2068227/1‑94 (MQ=60)
             TCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAC                                                                               <  SRR4302532.4993276/94‑1 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                              >  SRR4302532.6026299/1‑94 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                              >  SRR4302532.1740429/1‑94 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                              <  SRR4302532.1507998/94‑1 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                              <  SRR4302532.6152019/94‑1 (MQ=60)
               TTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCG                                                                             <  SRR4302532.5824433/94‑1 (MQ=60)
                TTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGC                                                                            <  SRR4302532.3972007/94‑1 (MQ=60)
                TTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGC                                                                            >  SRR4302532.2846317/1‑94 (MQ=60)
                 TTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCG                                                                           >  SRR4302532.3639712/1‑94 (MQ=60)
                 TTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCG                                                                           <  SRR4302532.4305741/94‑1 (MQ=60)
                      TTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCA                                                                      <  SRR4302532.1175355/94‑1 (MQ=60)
                      TTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCA                                                                      <  SRR4302532.4502464/94‑1 (MQ=60)
                         CGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCG                                                                   <  SRR4302532.6174739/94‑1 (MQ=60)
                          GCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGG                                                                  >  SRR4302532.2403101/1‑94 (MQ=60)
                          GCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGG                                                                  >  SRR4302532.4565360/1‑94 (MQ=60)
                           CAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGT                                                                 >  SRR4302532.1898316/1‑94 (MQ=60)
                            AAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTT                                                                >  SRR4302532.4600611/1‑94 (MQ=60)
                             AAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTA                                                               >  SRR4302532.2716753/1‑94 (MQ=60)
                              AGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTAC                                                              <  SRR4302532.5948245/94‑1 (MQ=60)
                               GGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACG                                                             <  SRR4302532.777852/94‑1 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGTTTGATGGGCGCGGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCA                                                          >  SRR4302532.2379522/1‑94 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCA                                                          >  SRR4302532.1904769/1‑94 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCA                                                          >  SRR4302532.901317/1‑94 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCA                                                          <  SRR4302532.2192980/94‑1 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCA                                                          >  SRR4302532.817774/1‑94 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGGTTGATGGGCGCATGTCGTACCGAACTGGTGAAAGTGTTCTACCTCGAACTACCGCGCACCAGCGGTTACGGCA                                                          >  SRR4302532.1475215/1‑94 (MQ=60)
                                    AATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACC                                                        >  SRR4302532.2371113/1‑94 (MQ=60)
                                    AATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGGCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACC                                                        >  SRR4302532.2371114/1‑94 (MQ=60)
                                     ATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCC                                                       >  SRR4302532.1593658/1‑94 (MQ=60)
                                     ATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCC                                                       >  SRR4302532.3926138/1‑94 (MQ=60)
                                       TCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTG                                                     <  SRR4302532.4217702/94‑1 (MQ=60)
                                        CTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAGGTGTTCTACGGCGCACTACCCCGAACCAGCGGTTACGTCACCCTGG                                                    >  SRR4302532.2887552/1‑94 (MQ=60)
                                        CTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGG                                                    >  SRR4302532.1617175/1‑94 (MQ=60)
                                        CTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGG                                                    >  SRR4302532.2728414/1‑94 (MQ=60)
                                         TTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGATACATAACCCTGGA                                                   >  SRR4302532.1406475/1‑94 (MQ=60)
                                           GGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATG                                                 <  SRR4302532.975230/94‑1 (MQ=60)
                                            GCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGG                                                <  SRR4302532.4029760/94‑1 (MQ=60)
                                             CGTCTCTTGTGGGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTATGGCGCACTACCGCGCACCAGCGTTTTCGTCACCCTTTATGGG                                               >  SRR4302532.5687802/1‑94 (MQ=60)
                                             CGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGG                                               >  SRR4302532.3232294/1‑94 (MQ=60)
                                             CGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGG                                               >  SRR4302532.4939835/1‑94 (MQ=60)
                                             CGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGG                                               <  SRR4302532.4475649/94‑1 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                              <  SRR4302532.5941824/94‑1 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                              <  SRR4302532.4873630/94‑1 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                              <  SRR4302532.1366913/94‑1 (MQ=60)
                                               tatcaGGTTTGATGGGCGCAGGTCGTACCGAACTGATGCAAGTGTTCTTCGGCGCACTACCGCGCACCAGCGGTTCAGACACCCTGGATGAGCA                                             >  SRR4302532.2047544/6‑94 (MQ=60)
                                               TCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCA                                             >  SRR4302532.2047550/1‑94 (MQ=60)
                                                CTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGTAT                                            >  SRR4302532.5851786/1‑94 (MQ=60)
                                                CTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCAT                                            <  SRR4302532.2671271/94‑1 (MQ=60)
                                                 TCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATG                                           >  SRR4302532.3858196/1‑94 (MQ=60)
                                                  CTGTTTTGCTGGGCGCAGGTCGTACCGATCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGA                                          <  SRR4302532.629502/94‑1 (MQ=60)
                                                  CTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGA                                          <  SRR4302532.629501/94‑1 (MQ=60)
                                                  CTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGAGTTCTACGGCGCACTACCGCGCACCCGCGGTTACGTCAACCTGGATGGGCATGA                                          >  SRR4302532.4620158/1‑94 (MQ=60)
                                                   TGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAA                                         <  SRR4302532.4819439/94‑1 (MQ=60)
                                                   TGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAA                                         >  SRR4302532.140832/1‑94 (MQ=60)
                                                   TGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAA                                         <  SRR4302532.4819604/94‑1 (MQ=60)
                                                    GGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTGCGTCACCCTGGATGGGCATGAAG                                        >  SRR4302532.3617079/1‑94 (MQ=60)
                                                    GGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAG                                        <  SRR4302532.303352/94‑1 (MQ=60)
                                                     GTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGT                                       <  SRR4302532.2294463/94‑1 (MQ=60)
                                                      TTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTC                                      <  SRR4302532.1834738/94‑1 (MQ=60)
                                                      TTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTC                                      <  SRR4302532.1060542/94‑1 (MQ=60)
                                                      TTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTC                                      <  SRR4302532.575248/94‑1 (MQ=60)
                                                       TTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCG                                     <  SRR4302532.1779106/94‑1 (MQ=60)
                                                       TTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCCCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCG                                     <  SRR4302532.6104035/94‑1 (MQ=60)
                                                        TGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTGACCCTGGATGGGCATGAAGTCGT                                    >  SRR4302532.3633836/1‑94 (MQ=60)
                                                        TGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGT                                    <  SRR4302532.3793449/94‑1 (MQ=60)
                                                         GATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTT                                   >  SRR4302532.2121840/1‑94 (MQ=60)
                                                          ATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTA                                  <  SRR4302532.2937487/94‑1 (MQ=60)
                                                           TGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAC                                 <  SRR4302532.4788079/94‑1 (MQ=60)
                                                           TGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAC                                 <  SRR4302532.4666524/94‑1 (MQ=60)
                                                            GGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACC                                <  SRR4302532.162096/94‑1 (MQ=60)
                                                            GGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAAC                                >  SRR4302532.322508/1‑94 (MQ=60)
                                                             GGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCC                               <  SRR4302532.2671632/94‑1 (MQ=60)
                                                             GGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCC                               <  SRR4302532.2932603/94‑1 (MQ=60)
                                                             GGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCC                               <  SRR4302532.4189912/94‑1 (MQ=60)
                                                                  CGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCA                          <  SRR4302532.5302769/94‑1 (MQ=60)
                                                                  AGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCA                          >  SRR4302532.1433219/1‑94 (MQ=60)
                                                                  AGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCA                          >  SRR4302532.849368/1‑94 (MQ=60)
                                                                   GGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAC                         >  SRR4302532.842658/1‑94 (MQ=60)
                                                                      CGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGC                      >  SRR4302532.4877023/1‑94 (MQ=60)
                                                                      CGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGC                      <  SRR4302532.1209027/94‑1 (MQ=60)
                                                                        TACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAG                    >  SRR4302532.2489720/1‑94 (MQ=60)
                                                                        TACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAG                    <  SRR4302532.3703473/94‑1 (MQ=60)
                                                                         ACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGG                   <  SRR4302532.3024713/94‑1 (MQ=60)
                                                                         ACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGG                   >  SRR4302532.1806157/1‑94 (MQ=60)
                                                                         ACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGG                   >  SRR4302532.1685603/1‑94 (MQ=60)
                                                                           CGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAT                 <  SRR4302532.1442610/94‑1 (MQ=60)
                                                                            GAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCTCCGCAGGATG                <  SRR4302532.4323339/94‑1 (MQ=60)
                                                                            GAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATG                >  SRR4302532.2090029/1‑94 (MQ=60)
                                                                            GAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATG                >  SRR4302532.5051209/1‑94 (MQ=60)
                                                                               CTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC             >  SRR4302532.6337208/1‑94 (MQ=60)
                                                                               CTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC             <  SRR4302532.295458/94‑1 (MQ=60)
                                                                               CTGATGAAAGTATTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC             <  SRR4302532.5767765/94‑1 (MQ=60)
                                                                               CTGATGAAAGGGTTCTACGGCTCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC             <  SRR4302532.295459/94‑1 (MQ=60)
                                                                                TGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCT            <  SRR4302532.1747815/94‑1 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG           >  SRR4302532.1022142/1‑94 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG           <  SRR4302532.2770694/94‑1 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG           >  SRR4302532.2508882/1‑94 (MQ=60)
                                                                                  ATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGG          >  SRR4302532.1419592/1‑94 (MQ=60)
                                                                                   TGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGC         >  SRR4302532.5339949/1‑94 (MQ=60)
                                                                                    GAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCA        >  SRR4302532.4878425/1‑94 (MQ=60)
                                                                                    GAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCA        <  SRR4302532.4162956/94‑1 (MQ=60)
                                                                                    GAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCA        >  SRR4302532.2161417/1‑94 (MQ=60)
                                                                                    GAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCA        >  SRR4302532.3230622/1‑94 (MQ=60)
                                                                                    GAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCA        >  SRR4302532.1072526/1‑94 (MQ=60)
                                                                                     AAAGTGTGCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAA       <  SRR4302532.4884076/94‑1 (MQ=60)
                                                                                      AAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAA      >  SRR4302532.2501724/1‑94 (MQ=60)
                                                                                      AAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAA      >  SRR4302532.4003819/1‑94 (MQ=60)
                                                                                       AGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAC     >  SRR4302532.5067651/1‑94 (MQ=60)
                                                                                        GTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACG    <  SRR4302532.3435395/94‑1 (MQ=60)
                                                                                         TGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGG   <  SRR4302532.5698693/94‑1 (MQ=60)
                                                                                         TGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGG   >  SRR4302532.3563545/1‑94 (MQ=60)
                                                                                          GTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGC  >  SRR4302532.4337955/1‑94 (MQ=60)
                                                                                           |                                                                                            
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGC  >  NC_000913/3932204‑3932387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑