Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,932,295 C→T L298F (CTC→TTC)  rbsA → ATP‑binding component of D‑ribose high‑affinity transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,932,2950CT100.0% 1056.4 / NA 281L298F (CTC→TTC) rbsAATP‑binding component of D‑ribose high‑affinity transport system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (162/119);  total (162/119)

Only 100 of 300 total aligned reads displayed.

GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAC  >  NC_000913/3932205‑3932384
                                                                                          |                                                                                         
gTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGttc                                                                                         <  1:1235582/93‑1 (MQ=255)
gTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGttc                                                                                         <  2:310221/93‑1 (MQ=255)
  tAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTa                                                                                       <  2:1887896/93‑1 (MQ=255)
   aaCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTAc                                                                                      >  2:2226407/1‑93 (MQ=255)
    aCGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACg                                                                                     >  1:2319688/1‑93 (MQ=255)
     cGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACgg                                                                                    >  1:551754/1‑93 (MQ=255)
      gATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgc                                                                                   >  2:2368584/1‑93 (MQ=255)
       aTGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgcg                                                                                  <  1:128599/93‑1 (MQ=255)
        tGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgcgc                                                                                 >  2:2507221/1‑93 (MQ=255)
        tGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGgcgc                                                                                 >  1:5639724/1‑93 (MQ=255)
         gTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCa                                                                                <  2:847851/93‑1 (MQ=255)
         gTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCa                                                                                >  1:4455405/1‑93 (MQ=255)
         gTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCa                                                                                >  2:5235852/1‑93 (MQ=255)
          tctcTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCAc                                                                               >  2:2134148/1‑93 (MQ=255)
           ctctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACt                                                                              <  2:3170878/93‑1 (MQ=255)
            tctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTa                                                                             >  1:3020655/1‑93 (MQ=255)
            tctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTa                                                                             >  1:5216182/1‑93 (MQ=255)
            tctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTa                                                                             >  1:3565600/1‑93 (MQ=255)
            tctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTATGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTa                                                                             >  2:4668407/1‑93 (MQ=255)
             ctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAc                                                                            <  2:6101046/93‑1 (MQ=255)
             ctTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAc                                                                            <  1:3208196/93‑1 (MQ=255)
              ttttACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAcc                                                                           >  1:6223727/1‑93 (MQ=255)
              ttttACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAcc                                                                           >  1:204224/1‑93 (MQ=255)
                ttactttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACcgc                                                                         <  1:693767/93‑1 (MQ=255)
                tgactttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATTGGCGCAGGTCGTGCCGAACTGATGAAAGTGTTCTACGGCGCACTACcgc                                                                         <  2:4396637/91‑1 (MQ=255)
                   ctttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCgcgcc                                                                      >  1:1640031/1‑92 (MQ=255)
                   ctttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCa                                                                      >  1:2084351/1‑93 (MQ=255)
                   ctttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCa                                                                      >  1:5452108/1‑93 (MQ=255)
                     ttacGCAAAGGCGAAATTCTTGTCGGCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCAcc                                                                    <  2:2012811/93‑1 (MQ=255)
                     ttacGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCAcc                                                                    >  1:4113343/1‑93 (MQ=255)
                        cGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGc                                                                 >  1:6572119/1‑93 (MQ=255)
                           aaaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGt                                                              >  2:107035/1‑93 (MQ=255)
                            aaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGtt                                                             <  1:2878004/93‑1 (MQ=255)
                            aaGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGtt                                                             <  2:3979193/93‑1 (MQ=255)
                             aGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTa                                                            <  2:5404765/93‑1 (MQ=255)
                              ggCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTAc                                                           >  2:2799414/1‑93 (MQ=255)
                               gCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACg                                                          >  2:5626996/1‑93 (MQ=255)
                                cGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGt                                                         >  2:5689984/1‑93 (MQ=255)
                                 gAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTc                                                        >  1:1877541/1‑93 (MQ=255)
                                  aaaTTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCa                                                       >  2:5091386/1‑93 (MQ=255)
                                   aaTTCTTGGCGTCTCTGGCTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGAGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAc                                                      >  1:5067403/1‑93 (MQ=255)
                                     ttCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAccc                                                    <  1:2260780/93‑1 (MQ=255)
                                     ttCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAccc                                                    >  1:2572130/1‑93 (MQ=255)
                                     ttCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAccc                                                    <  1:5001291/93‑1 (MQ=255)
                                        ttGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTgg                                                 >  1:4632296/1‑93 (MQ=255)
                                          ggCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGAt                                               >  2:3580822/1‑93 (MQ=255)
                                          ggCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGAt                                               <  1:4007593/93‑1 (MQ=255)
                                           gCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATg                                              <  2:2900039/93‑1 (MQ=255)
                                           gCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATg                                              <  2:150096/93‑1 (MQ=255)
                                            cGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATgg                                             <  2:6635137/93‑1 (MQ=255)
                                            cGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATgg                                             >  1:2465110/1‑93 (MQ=255)
                                              tctcTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGc                                           >  1:6363961/1‑93 (MQ=255)
                                              tctcTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGc                                           >  1:5833595/1‑93 (MQ=255)
                                              tctcTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCCGTTACGTCACCCTGGCTAGGc                                           >  1:4194065/1‑93 (MQ=255)
                                               ctctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCa                                          >  2:3245257/1‑93 (MQ=255)
                                               ctctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCa                                          <  1:3460387/93‑1 (MQ=255)
                                                tctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCAt                                         >  2:2717108/1‑93 (MQ=255)
                                                 ctGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATg                                        >  2:6568486/1‑93 (MQ=255)
                                                   ggTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGaa                                      >  1:3309941/1‑93 (MQ=255)
                                                       tGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCg                                  >  2:276406/1‑93 (MQ=255)
                                                        gATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGt                                 >  1:3791082/1‑93 (MQ=255)
                                                        gATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGt                                 <  2:4174330/93‑1 (MQ=255)
                                                        gATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGt                                 <  2:4294069/93‑1 (MQ=255)
                                                          tGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTa                               >  1:2512912/1‑93 (MQ=255)
                                                             gcgcAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAccc                            <  2:1577744/93‑1 (MQ=255)
                                                             gcgcAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAccc                            >  2:407380/1‑93 (MQ=255)
                                                             gcgcAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTAccc                            >  2:5752151/1‑93 (MQ=255)
                                                               gcAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGt                          >  2:1117554/1‑93 (MQ=255)
                                                                cAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGtt                         >  1:4820165/1‑93 (MQ=255)
                                                                  ggTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCa                       <  2:1305486/93‑1 (MQ=255)
                                                                   gTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAc                      >  1:1531628/1‑93 (MQ=255)
                                                                   gTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAc                      <  2:3873617/93‑1 (MQ=255)
                                                                   gTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAc                      >  1:5931804/1‑93 (MQ=255)
                                                                    tCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAcc                     >  2:4541686/1‑93 (MQ=255)
                                                                     cGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCg                    <  1:2798903/93‑1 (MQ=255)
                                                                      gTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGc                   >  2:4617952/1‑93 (MQ=255)
                                                                        aCCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAg                 <  2:5983161/93‑1 (MQ=255)
                                                                          cGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGa               >  2:1931850/1‑93 (MQ=255)
                                                                          cGAACTGATGAAAGTGTTCTACGCCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGa               <  2:5498054/93‑1 (MQ=255)
                                                                           gAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAt              <  2:944062/93‑1 (MQ=255)
                                                                           gAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAt              <  2:3904988/93‑1 (MQ=255)
                                                                           gAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAt              <  1:626562/93‑1 (MQ=255)
                                                                            aaCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATg             >  2:5139148/1‑93 (MQ=255)
                                                                            aaCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATg             >  1:1470402/1‑93 (MQ=255)
                                                                            aaCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATg             >  2:585307/1‑93 (MQ=255)
                                                                             aCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCTCCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATgg            <  2:3316531/93‑1 (MQ=255)
                                                                             aCTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATgg            <  2:1743374/93‑1 (MQ=255)
                                                                              cTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGc           <  1:2655256/93‑1 (MQ=255)
                                                                              cTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGCCACCCTGGATGGGCATGAAGTCTTTACCCGTTCACCGCAGGATGGc           <  1:5338113/93‑1 (MQ=255)
                                                                               tgatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGcc          >  2:3805064/1‑93 (MQ=255)
                                                                               tgatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGcc          <  2:1221637/93‑1 (MQ=255)
                                                                               tgatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGcc          >  1:3101764/1‑93 (MQ=255)
                                                                               tgatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGcc          <  1:5245280/93‑1 (MQ=255)
                                                                                gatgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCt         >  1:1015991/1‑93 (MQ=255)
                                                                                 atgaAGGTGTTCTACGGCGCACTACCGCGCACCAGCCGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTg        <  1:981101/93‑1 (MQ=255)
                                                                                 atgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTg        <  1:85563/93‑1 (MQ=255)
                                                                                  tgaAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCtcg       >  2:1427795/1‑91 (MQ=255)
                                                                                    aaaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCa     <  1:726626/93‑1 (MQ=255)
                                                                                     aaGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCaa    >  2:6457108/1‑93 (MQ=255)
                                                                                       gtgtTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAc  <  2:5608130/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAAC  >  NC_000913/3932205‑3932384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCA  >  NC_000913/3932204‑3932388
                                                                                           |                                                                                             
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTC                                                                                             <  SRR4302547.54285/94‑1 (MQ=60)
 GTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCT                                                                                            <  SRR4302547.1260513/94‑1 (MQ=60)
  TTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTA                                                                                           <  SRR4302547.5085083/94‑1 (MQ=60)
   TAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGGAAGTGTTCTAG                                                                                          >  SRR4302547.1732633/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                         <  SRR4302547.2121824/94‑1 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                         >  SRR4302547.132429/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                         >  SRR4302547.2366698/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                         >  SRR4302547.2187306/1‑94 (MQ=60)
    AACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACG                                                                                         >  SRR4302547.5711113/1‑94 (MQ=60)
     ACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGG                                                                                        >  SRR4302547.561702/1‑94 (MQ=60)
      CGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGC                                                                                       >  SRR4302547.2113723/1‑94 (MQ=60)
      CGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGC                                                                                       >  SRR4302547.6480506/1‑94 (MQ=60)
      CGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGC                                                                                       >  SRR4302547.3849251/1‑94 (MQ=60)
       GATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCG                                                                                      <  SRR4302547.3325833/94‑1 (MQ=60)
       GATGTCTCATTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCG                                                                                      >  SRR4302547.2579701/1‑94 (MQ=60)
        ATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGC                                                                                     >  SRR4302547.2615041/1‑94 (MQ=60)
        ATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGC                                                                                     >  SRR4302547.5756138/1‑94 (MQ=60)
        ATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGC                                                                                     <  SRR4302547.130927/94‑1 (MQ=60)
        ATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGC                                                                                     >  SRR4302547.1143375/1‑94 (MQ=60)
         TGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCA                                                                                    >  SRR4302547.723017/1‑94 (MQ=60)
         TGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCA                                                                                    >  SRR4302547.6463425/1‑94 (MQ=60)
         TGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCA                                                                                    >  SRR4302547.4547504/1‑94 (MQ=60)
           TCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACT                                                                                  <  SRR4302547.5143225/94‑1 (MQ=60)
            CTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTA                                                                                 >  SRR4302547.3080724/1‑94 (MQ=60)
            CTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTA                                                                                 >  SRR4302547.3638358/1‑94 (MQ=60)
            CTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTA                                                                                 <  SRR4302547.228097/94‑1 (MQ=60)
            CTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTA                                                                                 >  SRR4302547.4715844/1‑94 (MQ=60)
            CTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTA                                                                                 >  SRR4302547.5323548/1‑94 (MQ=60)
             TCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTAC                                                                                <  SRR4302547.6776960/94‑1 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTGCGGCGCACTACC                                                                               <  SRR4302547.4400489/94‑1 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                               <  SRR4302547.3272196/94‑1 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                               >  SRR4302547.3602044/1‑94 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                               >  SRR4302547.6352981/1‑94 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                               >  SRR4302547.4332000/1‑94 (MQ=60)
              CTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACC                                                                               >  SRR4302547.208044/1‑94 (MQ=60)
               TTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCG                                                                              <  SRR4302547.953497/94‑1 (MQ=60)
                 TTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCG                                                                            <  SRR4302547.706582/94‑1 (MQ=60)
                  TACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGC                                                                           >  SRR4302547.1115965/1‑94 (MQ=60)
                   ACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCC                                                                          >  SRR4302547.1672448/1‑94 (MQ=60)
                   ACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCA                                                                          >  SRR4302547.5564775/1‑94 (MQ=60)
                   ACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCA                                                                          >  SRR4302547.2125909/1‑94 (MQ=60)
                   ACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCA                                                                          >  SRR4302547.5321085/1‑94 (MQ=60)
                    CTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCAC                                                                         >  SRR4302547.3291142/1‑94 (MQ=60)
                    CTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCAC                                                                         >  SRR4302547.1669042/1‑94 (MQ=60)
                     TTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACC                                                                        >  SRR4302547.4196941/1‑94 (MQ=60)
                     TTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACC                                                                        >  SRR4302547.4232935/1‑94 (MQ=60)
                      TTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCA                                                                       >  SRR4302547.5549572/1‑94 (MQ=60)
                        acgaaagggcgaattttttggctcttgtggttggagGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGC                                                                     <  SRR4302547.4493776/58‑1 (MQ=60)
                        ACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGC                                                                     <  SRR4302547.5447779/94‑1 (MQ=60)
                        ACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGC                                                                     >  SRR4302547.4640483/1‑94 (MQ=60)
                        ACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGC                                                                     >  SRR4302547.6708947/1‑94 (MQ=60)
                          GCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGG                                                                   <  SRR4302547.2526665/94‑1 (MQ=60)
                           CAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGT                                                                  <  SRR4302547.5412286/94‑1 (MQ=60)
                            AAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTT                                                                 >  SRR4302547.4240529/1‑94 (MQ=60)
                            AAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTT                                                                 <  SRR4302547.3460904/94‑1 (MQ=60)
                             AAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTA                                                                <  SRR4302547.2935457/94‑1 (MQ=60)
                               GGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACG                                                              <  SRR4302547.2313068/94‑1 (MQ=60)
                               GGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACG                                                              >  SRR4302547.1525991/1‑94 (MQ=60)
                                 CGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTC                                                            >  SRR4302547.1914639/1‑94 (MQ=60)
                                  GAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCA                                                           >  SRR4302547.6187043/1‑94 (MQ=60)
                                   AAATTCTTGGCGTCTCTGGCTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGAGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCAC                                                          >  SRR4302547.5171959/1‑94 (MQ=60)
                                    AATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTCCCGCGCACCAGCGGTTACGTCACC                                                         <  SRR4302547.1834700/94‑1 (MQ=60)
                                    AATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACC                                                         >  SRR4302547.2435818/1‑94 (MQ=60)
                                     ATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCC                                                        >  SRR4302547.2624061/1‑94 (MQ=60)
                                      TTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCT                                                       <  SRR4302547.5104682/94‑1 (MQ=60)
                                      TTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCT                                                       <  SRR4302547.2306455/94‑1 (MQ=60)
                                      TTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCT                                                       <  SRR4302547.681803/94‑1 (MQ=60)
                                      ATCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCT                                                       <  SRR4302547.5522724/94‑1 (MQ=60)
                                        CTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGG                                                     >  SRR4302547.4728244/1‑94 (MQ=60)
                                         TTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGA                                                    >  SRR4302547.5328318/1‑94 (MQ=60)
                                           GGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATT                                                  <  SRR4302547.3505490/94‑1 (MQ=60)
                                           GGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATG                                                  >  SRR4302547.5211616/1‑94 (MQ=60)
                                           GGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATG                                                  <  SRR4302547.4089049/94‑1 (MQ=60)
                                            GCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGG                                                 >  SRR4302547.2514931/1‑94 (MQ=60)
                                            GCGTCGCTGTTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTTTTCTACGGCTCACTACCGCGCACCAGCGCTTACGTCACCCTGGATGG                                                 <  SRR4302547.2571453/94‑1 (MQ=60)
                                             CGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGG                                                >  SRR4302547.6377335/1‑94 (MQ=60)
                                             CGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGG                                                >  SRR4302547.1489423/1‑94 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                               >  SRR4302547.6496726/1‑94 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                               >  SRR4302547.5954296/1‑94 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                               >  SRR4302547.1993270/1‑94 (MQ=60)
                                              GTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCCGTTACGTCACCCTGGCTAGGC                                               >  SRR4302547.4279593/1‑94 (MQ=60)
                                              GTCTCTGGTTTGATGGCCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGC                                               <  SRR4302547.5616392/94‑1 (MQ=60)
                                               TCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCA                                              >  SRR4302547.1266324/1‑94 (MQ=60)
                                                CTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCAT                                             <  SRR4302547.4979576/94‑1 (MQ=60)
                                                CTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCAT                                             >  SRR4302547.126522/1‑94 (MQ=60)
                                                CTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCAT                                             <  SRR4302547.3530942/94‑1 (MQ=60)
                                                 TCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATG                                            >  SRR4302547.3268447/1‑94 (MQ=60)
                                                  CTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGA                                           >  SRR4302547.3872805/1‑94 (MQ=60)
                                                  CTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGA                                           <  SRR4302547.606403/94‑1 (MQ=60)
                                                   TGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAA                                          >  SRR4302547.3376682/1‑94 (MQ=60)
                                                   TGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAA                                          >  SRR4302547.1643631/1‑94 (MQ=60)
                                                    GGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAG                                         >  SRR4302547.2866324/1‑94 (MQ=60)
                                                    GGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAG                                         <  SRR4302547.6536528/94‑1 (MQ=60)
                                                     GTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGT                                        >  SRR4302547.418116/1‑94 (MQ=60)
                                                      TTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTC                                       >  SRR4302547.997579/1‑94 (MQ=60)
                                                        TGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGT                                     >  SRR4302547.847533/1‑94 (MQ=60)
                                                        TGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGT                                     >  SRR4302547.3868381/1‑94 (MQ=60)
                                                        TGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGT                                     >  SRR4302547.2561870/1‑94 (MQ=60)
                                                         GATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTT                                    <  SRR4302547.5335759/94‑1 (MQ=60)
                                                         AATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTT                                    <  SRR4302547.3193740/94‑1 (MQ=60)
                                                          ATGGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTA                                   >  SRR4302547.2563707/1‑94 (MQ=60)
                                                            GGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACC                                 <  SRR4302547.734288/94‑1 (MQ=60)
                                                            GGGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACC                                 >  SRR4302547.5790491/1‑94 (MQ=60)
                                                             GGCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCC                                >  SRR4302547.767785/1‑94 (MQ=60)
                                                              GCGCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCG                               >  SRR4302547.1561770/1‑94 (MQ=60)
                                                                GCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTT                             >  SRR4302547.4920055/1‑94 (MQ=60)
                                                                GCAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTT                             <  SRR4302547.5674815/94‑1 (MQ=60)
                                                                 CAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTC                            <  SRR4302547.3873042/94‑1 (MQ=60)
                                                                 CAGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTC                            <  SRR4302547.2805767/94‑1 (MQ=60)
                                                                  AGGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCA                           >  SRR4302547.3101775/1‑94 (MQ=60)
                                                                   GGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAC                          >  SRR4302547.1562118/1‑94 (MQ=60)
                                                                   GGTCGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCAC                          >  SRR4302547.6054780/1‑94 (MQ=60)
                                                                      CGTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGC                       <  SRR4302547.2854942/94‑1 (MQ=60)
                                                                       GTACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCA                      >  SRR4302547.2930584/1‑94 (MQ=60)
                                                                         ACCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGG                    >  SRR4302547.5875973/1‑94 (MQ=60)
                                                                          CCGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGA                   <  SRR4302547.6037528/94‑1 (MQ=60)
                                                                           CGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAT                  <  SRR4302547.5152806/94‑1 (MQ=60)
                                                                           CGAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGAT                  <  SRR4302547.602610/94‑1 (MQ=60)
                                                                            GAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATG                 <  SRR4302547.638130/94‑1 (MQ=60)
                                                                            GAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATG                 >  SRR4302547.1499774/1‑94 (MQ=60)
                                                                            GAACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATG                 >  SRR4302547.4889404/1‑94 (MQ=60)
                                                                             AACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGG                <  SRR4302547.3723465/94‑1 (MQ=60)
                                                                             AACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGG                <  SRR4302547.264576/94‑1 (MQ=60)
                                                                             AACTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGG                >  SRR4302547.195604/1‑94 (MQ=60)
                                                                               CTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC              <  SRR4302547.2708625/94‑1 (MQ=60)
                                                                               CTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC              >  SRR4302547.3163189/1‑94 (MQ=60)
                                                                               CTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCC              <  SRR4302547.2355631/94‑1 (MQ=60)
                                                                               CTGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGCCACCCTGGATGGGCATGAAGTCTTTACCCGTTCACCGCAGGATGGCC              <  SRR4302547.5448153/94‑1 (MQ=60)
                                                                                TGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCT             >  SRR4302547.1035887/1‑94 (MQ=60)
                                                                                TGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCT             <  SRR4302547.5353240/94‑1 (MQ=60)
                                                                                TGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCT             <  SRR4302547.356641/94‑1 (MQ=60)
                                                                                TGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCT             >  SRR4302547.304597/1‑94 (MQ=60)
                                                                                TGATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCT             <  SRR4302547.4918832/94‑1 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG            >  SRR4302547.3143747/1‑94 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG            <  SRR4302547.2794465/94‑1 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG            >  SRR4302547.2692293/1‑94 (MQ=60)
                                                                                 GATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTG            <  SRR4302547.4176144/94‑1 (MQ=60)
                                                                                  ATGAAGGTGTTCTACGGCGCACTACCGCGCACCAGCCGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGG           <  SRR4302547.1000194/94‑1 (MQ=60)
                                                                                  ATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGG           <  SRR4302547.6400189/94‑1 (MQ=60)
                                                                                  ATGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGG           <  SRR4302547.87143/94‑1 (MQ=60)
                                                                                   TGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGC          <  SRR4302547.2374477/94‑1 (MQ=60)
                                                                                   TGAAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGC          >  SRR4302547.5610199/1‑94 (MQ=60)
                                                                                     AAAGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAA        <  SRR4302547.740176/94‑1 (MQ=60)
                                                                                       AGTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGTCTGGCAAAC      <  SRR4302547.6636283/94‑1 (MQ=60)
                                                                                        GTGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACG     >  SRR4302547.5354685/1‑94 (MQ=60)
                                                                                         TGTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGG    >  SRR4302547.4434497/1‑94 (MQ=60)
                                                                                          GTTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGC   >  SRR4302547.2135718/1‑94 (MQ=60)
                                                                                           TTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCA  >  SRR4302547.925460/1‑94 (MQ=60)
                                                                                           TTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCA  >  SRR4302547.3248992/1‑94 (MQ=60)
                                                                                           |                                                                                             
CGTTAACGATGTCTCTTTTACTTTACGCAAAGGCGAAATTCTTGGCGTCTCTGGTTTGATGGGCGCAGGTCGTACCGAACTGATGAAAGTGCTCTACGGCGCACTACCGCGCACCAGCGGTTACGTCACCCTGGATGGGCATGAAGTCGTTACCCGTTCACCGCAGGATGGCCTGGCAAACGGCA  >  NC_000913/3932204‑3932388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑