Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,478	C→T	P116L (CCG→CTG)	ilvD →	dihydroxyacid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,478	0	C	T	98.6%	773.0 / ‑2.8	208	P116L (CCG→CTG)	ilvD	dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (111/93); total (114/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 215 total aligned reads displayed.

GCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG  >  NC_000913/3951388‑3951567

gcgAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTgg                                                                                         >  2:5984069/1‑93 (MQ=255)

   aaCTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGa                                                                                      >  1:1990026/1‑93 (MQ=255)

    aCTGATCGCTGATTCCGTTGAGTCTATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGAt                                                                                     <  1:3498230/93‑1 (MQ=255)

      tGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGc                                                                                   <  1:4078889/93‑1 (MQ=255)

        aTCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTg                                                                                 <  2:2495224/93‑1 (MQ=255)

         tCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGa                                                                                >  2:4502528/1‑93 (MQ=255)

         tCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGa                                                                                <  1:3025995/93‑1 (MQ=255)

         tCGCTGATTCCGTTGAGTATATGGTCAACGCCATCTGCGCCAACGCCATGGTCTGCATCTCTATCTGCGACAAGATCACCCTGGGGAAGCTgg                                                                                <  2:5468348/93‑2 (MQ=255)

          cGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACACAATCACCCTGGGGATGCTGAt                                                                               >  2:1532258/1‑93 (MQ=255)

          cGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGAt                                                                               <  1:283509/93‑1 (MQ=255)

          cGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGAt                                                                               >  1:2628645/1‑93 (MQ=255)

          cGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCAGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGAt                                                                               <  1:4257598/93‑1 (MQ=255)

           gCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATTACCCTGGGGATGCTGATg                                                                              <  2:2681362/93‑1 (MQ=255)

           gCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATg                                                                              >  1:4976654/1‑93 (MQ=255)

            cTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATgg                                                                             >  1:5281530/1‑93 (MQ=255)

            cTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATgg                                                                             >  1:3870571/1‑93 (MQ=255)

            cTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATAGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATgg                                                                             <  1:3078996/93‑1 (MQ=255)

               aTTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCCTGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCtt                                                                          >  1:5918688/1‑93 (MQ=255)

               aTTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCtt                                                                          <  2:2132807/93‑1 (MQ=255)

               aTTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCtt                                                                          >  1:3760355/1‑93 (MQ=255)

                ttCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGATTc                                                                         >  2:4377389/1‑93 (MQ=255)

                 tCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTcc                                                                        <  2:6084593/93‑1 (MQ=255)

                  ccGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTccc                                                                       <  1:3285289/93‑1 (MQ=255)

                  ccGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTccc                                                                       <  2:239341/93‑1 (MQ=255)

                  ccGTTGAGTATATGGTCAACCCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTccc                                                                       <  1:2415150/93‑1 (MQ=255)

                    gTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTg                                                                     <  1:2875379/93‑1 (MQ=255)

                    gTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTg                                                                     >  1:4541808/1‑93 (MQ=255)

                      tGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTgcg                                                                   >  2:5868452/1‑93 (MQ=255)

                       gAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTgcgc                                                                  >  2:671095/1‑93 (MQ=255)

                        aGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGcc                                                                 >  2:5263214/1‑93 (MQ=255)

                         gTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCt                                                                >  2:5407616/1‑93 (MQ=255)

                         gTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCt                                                                <  1:3658557/93‑1 (MQ=255)

                          tataTGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTg                                                               >  1:2264952/1‑93 (MQ=255)

                          tataTGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTg                                                               >  1:3158149/1‑93 (MQ=255)

                           atatGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGa                                                              >  1:2138781/1‑93 (MQ=255)

                            tatGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGaa                                                             <  2:797427/93‑1 (MQ=255)

                             atGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAat                                                            >  1:5365813/1‑93 (MQ=255)

                              tGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAata                                                           <  2:2896452/93‑1 (MQ=255)

                              tGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAata                                                           <  2:1075675/93‑1 (MQ=255)

                               ggTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAatat                                                          >  2:2691429/1‑93 (MQ=255)

                                 tCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTc                                                        <  2:140302/93‑1 (MQ=255)

                                  cAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTcc                                                       <  2:5789132/93‑1 (MQ=255)

                                  cAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTcc                                                       <  2:1747385/93‑1 (MQ=255)

                                  cAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTcc                                                       <  2:2027764/93‑1 (MQ=255)

                                  cAACGCCCACTCGGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCCCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTcc                                                       <  2:3036584/93‑1 (MQ=255)

                                   aaCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCg                                                      >  2:4861680/1‑93 (MQ=255)

                                   aaCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCg                                                      <  2:3112833/93‑1 (MQ=255)

                                   aaCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCg                                                      <  2:470707/93‑1 (MQ=255)

                                     cGCTCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGt                                                    <  2:5098379/93‑1 (MQ=255)

                                     cGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGt                                                    <  2:362877/93‑1 (MQ=255)

                                     cGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGt                                                    <  1:3546543/93‑1 (MQ=255)

                                       cccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGa                                                  <  1:3614358/93‑1 (MQ=255)

                                        ccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAt                                                 <  1:5844710/93‑1 (MQ=255)

                                        ccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAt                                                 >  1:4817354/1‑93 (MQ=255)

                                         cACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATc                                                >  2:2760794/1‑93 (MQ=255)

                                          aCTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCt                                               >  1:6147219/1‑93 (MQ=255)

                                          aCTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCt                                               >  2:307353/1‑93 (MQ=255)

                                          aCTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCt                                               <  1:4611235/93‑1 (MQ=255)

                                          aCTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCt                                               <  1:5121767/93‑1 (MQ=255)

                                           cTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCtt                                              >  2:2840790/1‑93 (MQ=255)

                                           cTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCtt                                              <  1:5642725/93‑1 (MQ=255)

                                             gcgcCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTg                                            >  2:3861853/1‑93 (MQ=255)

                                                ccGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGTTCTTTGttt                                         <  1:4896507/93‑1 (MQ=255)

                                                 cGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTc                                        >  1:5760712/1‑93 (MQ=255)

                                                  gACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTcc                                       >  2:3918784/1‑93 (MQ=255)

                                                  gACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTcc                                       <  1:1017588/93‑1 (MQ=255)

                                                   aCGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcg                                      >  1:3108983/1‑93 (MQ=255)

                                                   aCGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcg                                      <  2:3367138/93‑1 (MQ=255)

                                                    cGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcgg                                     <  2:5225695/93‑1 (MQ=255)

                                                     gCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggc                                    <  1:5569746/93‑1 (MQ=255)

                                                       cATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcgg                                  <  1:4431465/93‑1 (MQ=255)

                                                       cATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcgg                                  >  2:3377249/1‑93 (MQ=255)

                                                        aTGGTCTGCATCTCTAACTGCGACATAATCACCCTGGGGAAGCCGATGGCTTCCCTGCGTCAGAATATGCCGGCGATCTTTGATTCCggcggc                                 >  1:5454679/1‑93 (MQ=255)

                                                          ggTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGccc                               >  2:3985750/1‑93 (MQ=255)

                                                             cTGCATCTCTAACTGCGACAAAATCTCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGAt                            <  1:49565/93‑1 (MQ=255)

                                                               gCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATgg                          <  1:3325880/93‑1 (MQ=255)

                                                                cATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGa                         <  1:189731/93‑1 (MQ=255)

                                                                cATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGa                         <  1:915584/93‑1 (MQ=255)

                                                                cATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGa                         <  2:5040893/93‑1 (MQ=255)

                                                                 aTCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAg                        >  2:354057/1‑93 (MQ=255)

                                                                  tctcTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAgg                       >  1:4288331/1‑93 (MQ=255)

                                                                  tctcTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAgg                       >  2:227072/1‑93 (MQ=255)

                                                                   ctctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGc                      >  1:2205067/1‑93 (MQ=255)

                                                                    tctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGcc                     >  1:1883360/1‑93 (MQ=255)

                                                                      tAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCgg                   <  1:1525770/93‑1 (MQ=255)

                                                                       aaCTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCggg                  >  1:5521595/1‑93 (MQ=255)

                                                                        aCTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGa                 <  1:962145/93‑1 (MQ=255)

                                                                        aCTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGa                 <  2:31519/93‑1 (MQ=255)

                                                                          tGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGaaa               >  1:6088202/1‑93 (MQ=255)

                                                                            cGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAAc             <  2:1543750/93‑1 (MQ=255)

                                                                             gACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAAcc            <  2:1194459/93‑1 (MQ=255)

                                                                              aCAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCa           <  1:970701/93‑1 (MQ=255)

                                                                                aaaaTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCaaa         >  1:139879/1‑93 (MQ=255)

                                                                                 aaaTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAAc        >  2:4148529/1‑93 (MQ=255)

                                                                                   aTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACtt      <  2:344682/93‑1 (MQ=255)

                                                                                   aTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACtt      <  2:3230989/93‑1 (MQ=255)

                                                                                    tCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACttt     >  2:1891889/1‑93 (MQ=255)

                                                                                     cACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTc    <  2:1689695/93‑1 (MQ=255)

                                                                                      cccctGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTcc   <  1:818932/92‑1 (MQ=255)

                                                                                      aCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTcc   >  1:2891932/1‑93 (MQ=255)

GCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG  >  NC_000913/3951388‑3951567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCC‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGAT  >  NC_000913/3951385‑3951569

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT                                                                                              <  SRR4302532.205492/94‑1 (MQ=60)

 TCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑G                                                                                            <  SRR4302532.4492362/94‑1 (MQ=60)

     GAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGA                                                                                        >  SRR4302532.2056189/1‑94 (MQ=60)

      AACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGAT                                                                                       >  SRR4302532.3188576/1‑94 (MQ=60)

       ACTGATCGCTGATTCCGTTGAGTCTATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATG                                                                                      <  SRR4302532.3615432/94‑1 (MQ=60)

         TGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCT                                                                                    <  SRR4302532.4215855/94‑1 (MQ=60)

          GATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCCAACTGCGACAAAATCACCCT‑GGGGATGCTG                                                                                   >  SRR4302532.57334/1‑94 (MQ=60)

            TCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGAT                                                                                 <  SRR4302532.3125816/94‑1 (MQ=60)

            TCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGAT                                                                                 <  SRR4302532.3245508/94‑1 (MQ=60)

            TCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGAT                                                                                 >  SRR4302532.2716689/1‑94 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATG                                                                                >  SRR4302532.4867512/1‑94 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATG                                                                                <  SRR4302532.293308/94‑1 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATG                                                                                <  SRR4302532.371337/94‑1 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATG                                                                                >  SRR4302532.5147255/1‑94 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCAGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATG                                                                                <  SRR4302532.4400820/94‑1 (MQ=60)

              GCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGG                                                                               >  SRR4302532.3331294/1‑94 (MQ=60)

              GCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGG                                                                               >  SRR4302532.4001182/1‑94 (MQ=60)

              GCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGG                                                                               <  SRR4302532.937835/94‑1 (MQ=60)

              GCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGG                                                                               >  SRR4302532.5463688/1‑94 (MQ=60)

               CTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGC                                                                              >  SRR4302532.3976971/1‑94 (MQ=60)

               CTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATAGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGC                                                                              <  SRR4302532.3180757/94‑1 (MQ=60)

                TGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCT                                                                             >  SRR4302532.1019065/1‑94 (MQ=60)

                TGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCT                                                                             <  SRR4302532.2966517/94‑1 (MQ=60)

                 GATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCCTGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTT                                                                            >  SRR4302532.6123661/1‑94 (MQ=60)

                 GATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTT                                                                            >  SRR4302532.3886870/1‑94 (MQ=60)

                    TCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCC                                                                         >  SRR4302532.3128623/1‑94 (MQ=60)

                     CCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCT                                                                        <  SRR4302532.3394577/94‑1 (MQ=60)

                     CCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCT                                                                        >  SRR4302532.3764797/1‑94 (MQ=60)

                     CCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCT                                                                        >  SRR4302532.3640704/1‑94 (MQ=60)

                     CCGTTGAGTATATGGTCAACCCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCT                                                                        <  SRR4302532.2495477/94‑1 (MQ=60)

                      CGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTG                                                                       >  SRR4302532.4696837/1‑94 (MQ=60)

                      CGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTG                                                                       <  SRR4302532.1952009/94‑1 (MQ=60)

                       GTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGC                                                                      <  SRR4302532.2971527/94‑1 (MQ=60)

                         TGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGC                                                                    >  SRR4302532.2442344/1‑94 (MQ=60)

                         TGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGC                                                                    >  SRR4302532.2210949/1‑94 (MQ=60)

                          GAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCC                                                                   >  SRR4302532.512916/1‑94 (MQ=60)

                           AGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCT                                                                  >  SRR4302532.4413636/1‑94 (MQ=60)

                            GTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTG                                                                 <  SRR4302532.3781392/94‑1 (MQ=60)

                            GTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTG                                                                 >  SRR4302532.3262989/1‑94 (MQ=60)

                            GTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTG                                                                 >  SRR4302532.2340122/1‑94 (MQ=60)

                             TATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGA                                                                >  SRR4302532.2210207/1‑94 (MQ=60)

                              ATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAA                                                               >  SRR4302532.3424541/1‑94 (MQ=60)

                               TATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAAT                                                              >  SRR4302532.1001964/1‑94 (MQ=60)

                               TATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAAT                                                              >  SRR4302532.5551797/1‑94 (MQ=60)

                               TATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAAT                                                              <  SRR4302532.5615636/94‑1 (MQ=60)

                                   GTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTC                                                          <  SRR4302532.704674/94‑1 (MQ=60)

                                    TCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCC                                                         >  SRR4302532.5109916/1‑94 (MQ=60)

                                    CCAACCCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCC                                                         <  SRR4302532.3804359/94‑1 (MQ=60)

                                     CAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCG                                                        >  SRR4302532.5131724/1‑94 (MQ=60)

                                      AACGTCCTCTTCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCTCCCT‑GGGGATGCTGATGGCTTCCCTGCGCCCGAATAAGCCGG                                                       >  SRR4302532.5647107/1‑94 (MQ=60)

                                      AACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGCATATTCCTG                                                       >  SRR4302532.1672538/1‑94 (MQ=60)

                                        CGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTG                                                     <  SRR4302532.3665213/94‑1 (MQ=60)

                                         GCCCTCTGCGCCGACGCAATGGTCTGCATCTCTAACTGTGACAAAACCGCCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGA                                                    <  SRR4302532.3739651/94‑1 (MQ=60)

                                         GCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGA                                                    >  SRR4302532.5242812/1‑94 (MQ=60)

                                         GCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGA                                                    >  SRR4302532.634179/1‑94 (MQ=60)

                                         GCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGA                                                    <  SRR4302532.6249235/94‑1 (MQ=60)

                                          CCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAT                                                   <  SRR4302532.3735241/94‑1 (MQ=60)

                                          CCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAT                                                   >  SRR4302532.4983225/1‑94 (MQ=60)

                                           CCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATC                                                  <  SRR4302532.6047546/94‑1 (MQ=60)

                                            CACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCT                                                 >  SRR4302532.6359237/1‑94 (MQ=60)

                                             ACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTT                                                <  SRR4302532.5297953/94‑1 (MQ=60)

                                             ACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTT                                                <  SRR4302532.4768451/94‑1 (MQ=60)

                                             ACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTT                                                >  SRR4302532.2626489/1‑94 (MQ=60)

                                              CTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTT                                               <  SRR4302532.5838897/94‑1 (MQ=60)

                                                GCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGT                                             >  SRR4302532.4171669/1‑94 (MQ=60)

                                                GCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGT                                             <  SRR4302532.3125411/94‑1 (MQ=60)

                                                  GCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTT                                           >  SRR4302532.2263013/1‑94 (MQ=60)

                                                  GCCGACACCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTT                                           >  SRR4302532.3011257/1‑94 (MQ=60)

                                                   CCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGTTCTTTGTTTC                                          <  SRR4302532.5064789/94‑1 (MQ=60)

                                                   CCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTC                                          >  SRR4302532.5961206/1‑94 (MQ=60)

                                                    CGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCC                                         <  SRR4302532.5434723/94‑1 (MQ=60)

                                                     GACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                        <  SRR4302532.1052125/94‑1 (MQ=60)

                                                     GACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                        >  SRR4302532.3212110/1‑94 (MQ=60)

                                                     GACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                        >  SRR4302532.4691872/1‑94 (MQ=60)

                                                        GCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCG                                     <  SRR4302532.5763158/94‑1 (MQ=60)

                                                          CATGGTCTGCATCTCTAACTGCGACATAATCACCCT‑GGGGAAGCCGATGGCTTCCCTGCGTCAGAATATGCCGGCGATCTTTGATTCCGGCGGC                                   >  SRR4302532.5643751/1‑94 (MQ=53)

                                                          CATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGC                                   >  SRR4302532.4738065/1‑94 (MQ=60)

                                                          CATGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGC                                   <  SRR4302532.4582454/94‑1 (MQ=60)

                                                            TGGTCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCC                                 >  SRR4302532.348644/1‑94 (MQ=60)

                                                               TCTGCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGAT                              >  SRR4302532.59901/1‑94 (MQ=60)

                                                                CTGCATCTCTAACTGCGACAAAATCTCCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATG                             <  SRR4302532.51324/94‑1 (MQ=60)

                                                                  GCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGA                           >  SRR4302532.5047208/1‑94 (MQ=60)

                                                                  GCATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGA                           <  SRR4302532.3436792/94‑1 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTTCGGCGGGCCGATGGAG                          >  SRR4302532.2978861/1‑94 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                          >  SRR4302532.6082346/1‑94 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                          <  SRR4302532.595396/94‑1 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                          <  SRR4302532.946909/94‑1 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                          >  SRR4302532.1467056/1‑94 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                          <  SRR4302532.196120/94‑1 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                          <  SRR4302532.196121/94‑1 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCT‑GAAGATGCTGATGACTTCCCTGCGCTAGAATATTCCGGTGATCTTTGTTTCCGTCCGCCCGATGGAG                          <  SRR4302532.195647/94‑1 (MQ=60)

                                                                    ATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGG                         <  SRR4302532.631138/94‑1 (MQ=60)

                                                                    ATCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGG                         >  SRR4302532.4432987/1‑94 (MQ=60)

                                                                     TCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGC                        <  SRR4302532.5785832/94‑1 (MQ=60)

                                                                     TCTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGC                        >  SRR4302532.2278411/1‑94 (MQ=60)

                                                                      CTCTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCC                       >  SRR4302532.1945806/1‑94 (MQ=60)

                                                                        CTAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGG                     <  SRR4302532.4495144/94‑1 (MQ=60)

                                                                         TAACTGCGACAAAATCGCCCC‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGG                    <  SRR4302532.2318848/94‑1 (MQ=60)

                                                                         TAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGG                    <  SRR4302532.1577305/94‑1 (MQ=60)

                                                                         TAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGG                    >  SRR4302532.5477410/1‑94 (MQ=60)

                                                                         TAACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGG                    >  SRR4302532.5713199/1‑94 (MQ=60)

                                                                          AACTGCGATAAAATCACCCG‑GAGGATGATGATGGATTCCCTGCGCCTGAATATTGCGGTGATCGTTGTTTCTGGCGGCACGATGGAGGCCGtgc                   >  SRR4302532.332634/1‑91 (MQ=47)

                                                                           ACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAA                  <  SRR4302532.994983/94‑1 (MQ=60)

                                                                           ACTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAA                  <  SRR4302532.4307638/94‑1 (MQ=60)

                                                                            CTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAA                 >  SRR4302532.2080830/1‑94 (MQ=60)

                                                                            CTGCGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAA                 >  SRR4302532.6298401/1‑94 (MQ=60)

                                                                               CGACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACC              >  SRR4302532.4349275/1‑94 (MQ=60)

                                                                                 ACAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAA            <  SRR4302532.1003815/94‑1 (MQ=60)

                                                                                  CAAAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAA           >  SRR4302532.144606/1‑94 (MQ=60)

                                                                                   AAAATCACCCT‑GGGTATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAAC          <  SRR4302532.970166/94‑1 (MQ=60)

                                                                                    AAATCACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACT         <  SRR4302532.284142/94‑1 (MQ=60)

                                                                                    AAATCACCCG‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACT         >  SRR4302532.5668998/1‑94 (MQ=60)

                                                                                        CACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCC     >  SRR4302532.2988533/1‑94 (MQ=60)

                                                                                         ACCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG    >  SRR4302532.1368374/1‑94 (MQ=60)

                                                                                          CCCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGA   >  SRR4302532.2968096/1‑94 (MQ=60)

                                                                                          CCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG    <  SRR4302532.846873/94‑1 (MQ=60)

                                                                                          CCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG    <  SRR4302532.3601613/94‑1 (MQ=60)

                                                                                           CCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGAT  >  SRR4302532.1929104/1‑94 (MQ=60)

                                                                                           CCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGAT  >  SRR4302532.5254297/1‑94 (MQ=60)

                                                                                           CCT‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTCTCCGAT  >  SRR4302532.2182517/1‑94 (MQ=60)

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCC‑GGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGAT  >  NC_000913/3951385‑3951569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`