Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,951,478	C→T	P116L (CCG→CTG)	ilvD →	dihydroxyacid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,951,478	0	C	T	100.0%	944.9 / NA	243	P116L (CCG→CTG)	ilvD	dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (129/114); total (129/114)

Only 100 of 251 total aligned reads displayed.

GCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG  >  NC_000913/3951388‑3951567

 cgAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTggg                                                                                        <  2:3729231/93‑1 (MQ=255)

 cgAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTggg                                                                                        <  1:1828697/93‑1 (MQ=255)

 cgAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTggg                                                                                        <  1:655793/93‑1 (MQ=255)

  gAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTgggg                                                                                       >  2:1535697/1‑93 (MQ=255)

   aaCTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGa                                                                                      >  2:6125261/1‑93 (MQ=255)

   aaCTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGa                                                                                      >  1:3405574/1‑93 (MQ=255)

   aaCTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGa                                                                                      <  2:2328393/93‑1 (MQ=255)

   aaCTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGa                                                                                      <  2:4967405/93‑1 (MQ=255)

    aCTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGAt                                                                                     >  2:4175223/1‑93 (MQ=255)

       gATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCt                                                                                  >  2:1230896/1‑93 (MQ=255)

        aTCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTg                                                                                 >  1:6094859/1‑93 (MQ=255)

        aTCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTg                                                                                 >  1:2675484/1‑93 (MQ=255)

        aTCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTg                                                                                 >  1:1004017/1‑93 (MQ=255)

              gATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCt                                                                           <  2:1595376/93‑1 (MQ=255)

               aTTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCtt                                                                          >  2:485662/1‑93 (MQ=255)

                ttCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTc                                                                         >  1:1630938/1‑93 (MQ=255)

                ttCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTc                                                                         >  1:4810406/1‑93 (MQ=255)

                 tCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTcc                                                                        <  2:520790/93‑1 (MQ=255)

                 tCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTcc                                                                        <  2:3469166/93‑1 (MQ=255)

                   cGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCt                                                                      <  1:5257283/93‑1 (MQ=255)

                    gTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTg                                                                     >  2:1145497/1‑93 (MQ=255)

                       gAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTgcgc                                                                  >  1:592127/1‑93 (MQ=255)

                          tataTGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTg                                                               >  2:5705476/1‑93 (MQ=255)

                          tataTGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTg                                                               >  2:2789558/1‑93 (MQ=255)

                           atatGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGa                                                              <  2:1926945/93‑1 (MQ=255)

                           atatGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGa                                                              >  1:6373557/1‑93 (MQ=255)

                            tatGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGaa                                                             >  2:587644/1‑93 (MQ=255)

                              tGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAata                                                           >  1:4361281/1‑93 (MQ=255)

                              tGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAata                                                           <  2:6328891/93‑1 (MQ=255)

                               ggTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAatat                                                          <  2:146155/93‑1 (MQ=255)

                               ggTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAatat                                                          >  2:4261518/1‑93 (MQ=255)

                                  cAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTcc                                                       <  2:1546932/93‑1 (MQ=255)

                                   aaCTCCCACTGCCCCGACGCTATGGTGTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGTTTCCCTGCGCCTGAATATTCCg                                                      <  1:5355814/93‑1 (MQ=255)

                                   aaCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCg                                                      >  1:3091186/1‑93 (MQ=255)

                                    aCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCgg                                                     >  2:4652358/1‑93 (MQ=255)

                                    aCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCgg                                                     >  2:2546332/1‑93 (MQ=255)

                                    aCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCgg                                                     >  1:6225754/1‑93 (MQ=255)

                                    aCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCgg                                                     >  2:4065915/1‑93 (MQ=255)

                                    aCGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCgg                                                     <  1:5052413/93‑1 (MQ=255)

                                       cccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGa                                                  >  1:3493025/1‑93 (MQ=255)

                                       cccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGa                                                  >  2:4911211/1‑93 (MQ=255)

                                       cccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGa                                                  >  2:2083108/1‑93 (MQ=255)

                                        ccACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAt                                                 >  2:607580/1‑93 (MQ=255)

                                         cACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATc                                                <  1:2989773/93‑1 (MQ=255)

                                          aCTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCt                                               >  1:4320070/1‑93 (MQ=255)

                                           cTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCtt                                              <  1:1724886/93‑1 (MQ=255)

                                           cTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCtt                                              >  2:6223781/1‑93 (MQ=255)

                                           cTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCtt                                              >  1:2162430/1‑93 (MQ=255)

                                              cgcCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGt                                           <  2:5600574/93‑1 (MQ=255)

                                                 cGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTc                                        >  1:14298/1‑93 (MQ=255)

                                                  gACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTcc                                       >  1:6148639/1‑93 (MQ=255)

                                                  gACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTcc                                       >  1:5554897/1‑93 (MQ=255)

                                                  gACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTcc                                       >  1:4587187/1‑93 (MQ=255)

                                                  gACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTcc                                       <  2:649145/93‑1 (MQ=255)

                                                   aCGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcg                                      >  1:4966875/1‑93 (MQ=255)

                                                   aCGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcg                                      <  1:4003130/93‑1 (MQ=255)

                                                   aCGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcg                                      >  1:197990/1‑93 (MQ=255)

                                                   aCGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcg                                      <  2:3357602/93‑1 (MQ=255)

                                                    cGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcgg                                     >  1:4471506/1‑93 (MQ=255)

                                                    cGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcgg                                     <  2:3606288/93‑1 (MQ=255)

                                                    cGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcgg                                     <  1:5226624/93‑1 (MQ=255)

                                                     gCCATGGTCTGCAGCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggc                                    <  1:2273295/93‑1 (MQ=255)

                                                      ccATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcg                                   >  1:1328495/1‑93 (MQ=255)

                                                      ccATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcg                                   >  1:3671743/1‑93 (MQ=255)

                                                      ccATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcg                                   <  2:6439100/93‑1 (MQ=255)

                                                      ccATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcg                                   >  1:2460002/1‑93 (MQ=255)

                                                       cATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCcggcgg                                  >  2:4472941/1‑93 (MQ=255)

                                                       cATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATGCCGGTGATCTTTGTTTCcggcgg                                  >  2:3269150/1‑93 (MQ=255)

                                                        aTGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCggcggc                                 >  2:4724459/1‑93 (MQ=255)

                                                         tGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGcc                                >  1:1181713/1‑93 (MQ=255)

                                                         tGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGcc                                <  2:5965328/93‑1 (MQ=255)

                                                         tGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGcc                                <  2:1057070/93‑1 (MQ=255)

                                                           gTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCg                              >  2:35073/1‑93 (MQ=255)

                                                           gTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCg                              <  1:4611195/93‑1 (MQ=255)

                                                            tCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGa                             >  1:6438211/1‑93 (MQ=255)

                                                                 aTCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAg                        <  2:283200/93‑1 (MQ=255)

                                                                 aTCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAg                        <  2:199359/93‑1 (MQ=255)

                                                                   ctctAACTGCGACAAAATCGCCCTGGGGATGCTGATGGCTTCGCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGc                      <  2:1699165/93‑1 (MQ=255)

                                                                   ctctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGc                      <  1:5802087/93‑1 (MQ=255)

                                                                    tctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGcc                     >  2:5383869/1‑93 (MQ=255)

                                                                    tctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGcc                     >  2:2655890/1‑93 (MQ=255)

                                                                    tctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGcc                     >  1:5630898/1‑93 (MQ=255)

                                                                     ctAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCg                    <  1:3885224/93‑1 (MQ=255)

                                                                        aCTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCTGCGGCCCGATGGAGGCCGGGa                 >  2:851470/1‑93 (MQ=255)

                                                                        aCTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGa                 >  1:3967223/1‑93 (MQ=255)

                                                                          tGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGaaa               >  2:1861666/1‑93 (MQ=255)

                                                                          cgCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGaaa               <  1:5131957/92‑1 (MQ=255)

                                                                           gCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGaaaa              >  1:875839/1‑93 (MQ=255)

                                                                              aCAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCa           >  2:3092223/1‑93 (MQ=255)

                                                                               cAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTAAATATTCCGGTGATCTTTGTTTCCGGCGTCCCGATGGAGGCCGGCAAAACCaa          <  1:6290865/93‑1 (MQ=255)

                                                                                aaaaTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCaaa         <  1:5857108/93‑1 (MQ=255)

                                                                                 aaaTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAAc        <  2:2883243/93‑1 (MQ=255)

                                                                                  aaTCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACt       <  2:3065314/93‑1 (MQ=255)

                                                                                   accACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACtt      <  1:1383358/91‑1 (MQ=255)

                                                                                    tCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACttt     <  1:2546332/93‑1 (MQ=255)

                                                                                    tCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACttt     <  1:2059695/93‑1 (MQ=255)

                                                                                     cACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTc    <  2:318033/93‑1 (MQ=255)

                                                                                      aCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTcc   <  1:3604671/93‑1 (MQ=255)

                                                                                      aCCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTcc   >  1:3272959/1‑93 (MQ=255)

                                                                                       ccctGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCg  >  2:2735105/1‑93 (MQ=255)

GCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG  >  NC_000913/3951388‑3951567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCA  >  NC_000913/3951385‑3951571

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT                                                                                               <  SRR4302547.487263/94‑1 (MQ=60)

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT                                                                                               <  SRR4302547.2785954/94‑1 (MQ=60)

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCT                                                                                               <  SRR4302547.2538084/94‑1 (MQ=60)

 TCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTG                                                                                              <  SRR4302547.2145493/94‑1 (MQ=60)

 TCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTG                                                                                              <  SRR4302547.650446/94‑1 (MQ=60)

 TCGCGAACTGATCGCTGATTCCGTAGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCCGCACCTCTAACTGCGACAAAATCCCCCTG                                                                                              <  SRR4302547.4214804/94‑1 (MQ=60)

   GCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGG                                                                                            >  SRR4302547.4481179/1‑94 (MQ=60)

   GCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGG                                                                                            <  SRR4302547.1986568/94‑1 (MQ=60)

    CGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGG                                                                                           <  SRR4302547.667869/94‑1 (MQ=60)

    CGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGG                                                                                           <  SRR4302547.1864845/94‑1 (MQ=60)

     GAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGA                                                                                          >  SRR4302547.3474868/1‑94 (MQ=60)

     GAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGA                                                                                          >  SRR4302547.6566225/1‑94 (MQ=60)

      AACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGAT                                                                                         <  SRR4302547.5098372/94‑1 (MQ=60)

       ACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATG                                                                                        <  SRR4302547.5645680/94‑1 (MQ=60)

        CTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGC                                                                                       <  SRR4302547.4416435/94‑1 (MQ=60)

        CTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGC                                                                                       >  SRR4302547.4706622/1‑94 (MQ=60)

          GATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTG                                                                                     <  SRR4302547.1437175/94‑1 (MQ=60)

          GATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTG                                                                                     >  SRR4302547.1023605/1‑94 (MQ=60)

          GATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTG                                                                                     >  SRR4302547.2729217/1‑94 (MQ=60)

          GATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTG                                                                                     >  SRR4302547.6221295/1‑94 (MQ=60)

           ATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGA                                                                                    >  SRR4302547.1718829/1‑94 (MQ=60)

           ATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGA                                                                                    >  SRR4302547.6201754/1‑94 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCTCCCTGGGGATGCTGATG                                                                                  <  SRR4302547.1935185/94‑1 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATG                                                                                  <  SRR4302547.3108486/94‑1 (MQ=60)

             CGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATG                                                                                  <  SRR4302547.6622094/94‑1 (MQ=60)

              GCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGG                                                                                 >  SRR4302547.6673628/1‑94 (MQ=60)

              GCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGG                                                                                 >  SRR4302547.1814970/1‑94 (MQ=60)

                TGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCT                                                                               >  SRR4302547.2152531/1‑94 (MQ=60)

                TGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCT                                                                               <  SRR4302547.6734564/94‑1 (MQ=60)

                  ATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTC                                                                             >  SRR4302547.4910108/1‑94 (MQ=60)

                  ATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTC                                                                             >  SRR4302547.1663227/1‑94 (MQ=60)

                    TCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCC                                                                           <  SRR4302547.6302963/94‑1 (MQ=60)

                    TCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCC                                                                           >  SRR4302547.1455129/1‑94 (MQ=60)

                      CGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTG                                                                         >  SRR4302547.6043304/1‑94 (MQ=60)

                      CGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTG                                                                         <  SRR4302547.5365536/94‑1 (MQ=60)

                      CGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTG                                                                         <  SRR4302547.2885368/94‑1 (MQ=60)

                        TTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCG                                                                       <  SRR4302547.3960208/94‑1 (MQ=60)

                         TGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGC                                                                      >  SRR4302547.602920/1‑94 (MQ=60)

                         TGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGC                                                                      >  SRR4302547.602921/1‑94 (MQ=60)

                          GAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCC                                                                     <  SRR4302547.6358863/94‑1 (MQ=60)

                           AGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCT                                                                    <  SRR4302547.1395565/94‑1 (MQ=60)

                           AGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCT                                                                    >  SRR4302547.5689157/1‑94 (MQ=60)

                             TATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGA                                                                  <  SRR4302547.4919782/94‑1 (MQ=60)

                             TATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGA                                                                  >  SRR4302547.6506534/1‑94 (MQ=60)

                              ATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAA                                                                 <  SRR4302547.509993/94‑1 (MQ=60)

                              ATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAA                                                                 >  SRR4302547.1310483/1‑94 (MQ=60)

                              ATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAA                                                                 >  SRR4302547.230730/1‑94 (MQ=60)

                               TATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAAT                                                                <  SRR4302547.3804440/94‑1 (MQ=60)

                                ATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATT                                                               >  SRR4302547.1598296/1‑94 (MQ=60)

                                ATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATA                                                               <  SRR4302547.2649596/94‑1 (MQ=60)

                                ATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATA                                                               <  SRR4302547.3453616/94‑1 (MQ=60)

                                ATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATA                                                               <  SRR4302547.244554/94‑1 (MQ=60)

                                ATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATA                                                               >  SRR4302547.4450856/1‑94 (MQ=60)

                                  GGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGTCGATGCTGATGGCTTCCCTGCGACTGAATATT                                                             >  SRR4302547.5352502/1‑94 (MQ=60)

                                   GTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTC                                                            >  SRR4302547.5923201/1‑94 (MQ=60)

                                     CAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCG                                                          >  SRR4302547.3152486/1‑94 (MQ=60)

                                      AACTCCCACTGCCCCGACGCTATGGTGTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGTTTCCCTGCGCCTGAATATTCCGG                                                         <  SRR4302547.5466265/94‑1 (MQ=60)

                                      AACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGG                                                         >  SRR4302547.6355057/1‑94 (MQ=60)

                                       gctcttccgatcttgacattATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGT                                                        >  SRR4302547.5398441/21‑94 (MQ=60)

                                       ACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGT                                                        <  SRR4302547.5156722/94‑1 (MQ=60)

                                       ACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGT                                                        <  SRR4302547.4675088/94‑1 (MQ=60)

                                        CGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTG                                                       <  SRR4302547.3721495/94‑1 (MQ=60)

                                        CGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTG                                                       <  SRR4302547.2609574/94‑1 (MQ=60)

                                         GCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGGTGGCTTCCCTGCGCCTGAATATTCCGGTGA                                                      >  SRR4302547.4093246/1‑94 (MQ=60)

                                         GCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGA                                                      >  SRR4302547.3564312/1‑94 (MQ=60)

                                          CCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAT                                                     >  SRR4302547.5236963/1‑94 (MQ=60)

                                          CCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGAT                                                     <  SRR4302547.3264620/94‑1 (MQ=60)

                                           CCACTGCGCCGACGCCATGGTCTGGATATCTAACTGCGACAAATTCACACTGGGGATGATGATGGGTTCCCAGGGCCTGGATATTCCGGTGATC                                                    >  SRR4302547.1071591/1‑94 (MQ=42)

                                            CACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCT                                                   >  SRR4302547.4409001/1‑94 (MQ=60)

                                            CACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCT                                                   <  SRR4302547.3049256/94‑1 (MQ=60)

                                             ACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTT                                                  >  SRR4302547.2205655/1‑94 (MQ=60)

                                              CTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTT                                                 <  SRR4302547.1758790/94‑1 (MQ=60)

                                              CTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTT                                                 <  SRR4302547.5334750/94‑1 (MQ=60)

                                              CTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTT                                                 <  SRR4302547.6488341/94‑1 (MQ=60)

                                               TGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTG                                                <  SRR4302547.252417/94‑1 (MQ=60)

                                                 CGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTT                                              <  SRR4302547.4427113/94‑1 (MQ=60)

                                                  GCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTT                                             <  SRR4302547.6269751/94‑1 (MQ=60)

                                                  GCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTT                                             >  SRR4302547.1696357/1‑94 (MQ=60)

                                                   CCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTC                                            >  SRR4302547.14563/1‑94 (MQ=60)

                                                    CGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCC                                           >  SRR4302547.4682129/1‑94 (MQ=60)

                                                    CGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCC                                           >  SRR4302547.5669489/1‑94 (MQ=60)

                                                    CGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCC                                           >  SRR4302547.6276204/1‑94 (MQ=60)

                                                     GACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                          >  SRR4302547.4881176/1‑94 (MQ=60)

                                                     GACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                          >  SRR4302547.201690/1‑94 (MQ=60)

                                                     GACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                          >  SRR4302547.5069655/1‑94 (MQ=60)

                                                     CACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCG                                          <  SRR4302547.4600917/94‑1 (MQ=60)

                                                      ACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGG                                         <  SRR4302547.4084500/94‑1 (MQ=60)

                                                      ACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGG                                         >  SRR4302547.4563893/1‑94 (MQ=60)

                                                       CGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGC                                        <  SRR4302547.5334206/94‑1 (MQ=60)

                                                        GCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCG                                       >  SRR4302547.2509717/1‑94 (MQ=60)

                                                        GCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCG                                       >  SRR4302547.1355198/1‑94 (MQ=60)

                                                        GCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCG                                       >  SRR4302547.3746624/1‑94 (MQ=60)

                                                        GCCATGGTCTGCAGCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCG                                       <  SRR4302547.2319269/94‑1 (MQ=60)

                                                          CATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGC                                     >  SRR4302547.2022931/1‑94 (MQ=60)

                                                           ATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCC                                    >  SRR4302547.3972636/1‑94 (MQ=60)

                                                           ATGGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCC                                    >  SRR4302547.1205586/1‑94 (MQ=60)

                                                             GGTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCA                                  <  SRR4302547.3650240/94‑1 (MQ=60)

                                                              GTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGA                                 >  SRR4302547.1331725/1‑94 (MQ=60)

                                                              GTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGA                                 >  SRR4302547.5776030/1‑94 (MQ=60)

                                                              GTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGA                                 <  SRR4302547.4706644/94‑1 (MQ=60)

                                                              GTCTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGA                                 >  SRR4302547.6572386/1‑94 (MQ=60)

                                                                CTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATG                               <  SRR4302547.3376891/94‑1 (MQ=60)

                                                                CTGCATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATG                               <  SRR4302547.5441476/94‑1 (MQ=60)

                                                                   CATCTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAG                            >  SRR4302547.2346530/1‑94 (MQ=60)

                                                                      CTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCC                         >  SRR4302547.6481833/1‑94 (MQ=60)

                                                                      CTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCC                         >  SRR4302547.5747107/1‑94 (MQ=60)

                                                                      CTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCC                         <  SRR4302547.6319218/94‑1 (MQ=60)

                                                                      CTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCC                         <  SRR4302547.5922123/94‑1 (MQ=60)

                                                                      CTCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCC                         <  SRR4302547.5301593/94‑1 (MQ=60)

                                                                       TCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCG                        >  SRR4302547.5311374/1‑94 (MQ=60)

                                                                       TCTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCG                        <  SRR4302547.4053887/94‑1 (MQ=60)

                                                                        CTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGG                       <  SRR4302547.3964257/94‑1 (MQ=60)

                                                                        CTAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGG                       <  SRR4302547.5956242/94‑1 (MQ=60)

                                                                         TAACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGG                      >  SRR4302547.2118745/1‑94 (MQ=60)

                                                                          AACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGA                     >  SRR4302547.5717479/1‑94 (MQ=60)

                                                                          AACTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGA                     >  SRR4302547.4047920/1‑94 (MQ=60)

                                                                            CTGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAA                   >  SRR4302547.3483017/1‑94 (MQ=60)

                                                                             TGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAA                  >  SRR4302547.892651/1‑94 (MQ=60)

                                                                             CGCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAA                  <  SRR4302547.5237691/94‑1 (MQ=60)

                                                                              GCGACAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAAC                 <  SRR4302547.3093612/94‑1 (MQ=60)

                                                                                  CAAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTAAATATTCCGGTGATCTTTGTTTCCGGCGTCCCGATGGAGGCCGGCAAAACCAAA             <  SRR4302547.6421601/94‑1 (MQ=60)

                                                                                   AAAATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAAC            <  SRR4302547.5978298/94‑1 (MQ=60)

                                                                                     AATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTT          >  SRR4302547.973769/1‑94 (MQ=60)

                                                                                      ATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGTCCCGATGGAGGCCGGGAAAACCAAACTTT         <  SRR4302547.1168654/94‑1 (MQ=60)

                                                                                      ATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTT         >  SRR4302547.408/1‑94 (MQ=60)

                                                                                      ATCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTT         <  SRR4302547.4835255/94‑1 (MQ=60)

                                                                                      ACCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTT         <  SRR4302547.1411064/94‑1 (MQ=60)

                                                                                       TCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTC        <  SRR4302547.5445793/94‑1 (MQ=60)

                                                                                       TCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTC        <  SRR4302547.2597757/94‑1 (MQ=60)

                                                                                       TCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTC        >  SRR4302547.3621693/1‑94 (MQ=60)

                                                                                       TCACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTC        <  SRR4302547.2100796/94‑1 (MQ=60)

                                                                                        CACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCC       >  SRR4302547.3338508/1‑94 (MQ=60)

                                                                                         ACCCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCG      <  SRR4302547.3678180/94‑1 (MQ=60)

                                                                                           CCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGAT    <  SRR4302547.5079802/94‑1 (MQ=60)

                                                                                           CCTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGAT    >  SRR4302547.3833880/1‑94 (MQ=60)

                                                                                            CTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATC   <  SRR4302547.6456824/94‑1 (MQ=60)

                                                                                            CTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATC   >  SRR4302547.5730616/1‑94 (MQ=60)

                                                                                            CTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATC   <  SRR4302547.6330119/94‑1 (MQ=60)

                                                                                            CTGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTACCGGCGTCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATC   <  SRR4302547.174529/94‑1 (MQ=60)

                                                                                             TGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCA  >  SRR4302547.6582155/1‑94 (MQ=60)

                                                                                             TGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCA  <  SRR4302547.2000110/94‑1 (MQ=60)

CTCGCGAACTGATCGCTGATTCCGTTGAGTATATGGTCAACGCCCACTGCGCCGACGCCATGGTCTGCATCTCTAACTGCGACAAAATCACCCCGGGGATGCTGATGGCTTCCCTGCGCCTGAATATTCCGGTGATCTTTGTTTCCGGCGGCCCGATGGAGGCCGGGAAAACCAAACTTTCCGATCA  >  NC_000913/3951385‑3951571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`