Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,958,877 2 bp→CG coding (586‑587/2022 nt) rep → rep helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,958,8770GC100.0% 882.5 / NA 242A196R (GCC→CGC) reprep helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (133/109);  total (133/109)
*NC_0009133,958,8780CG100.0% 886.6 / NA 243A196R (GCC→CGC) reprep helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (133/110);  total (133/110)

Only 100 of 246 total aligned reads displayed.

TGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958790‑3958964
                                                                                       ||                                                                                      
   tATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTACTGCCGACGTTGCTGCTGCAACGCAATgaa                                                                                 <  1:1141823/93‑1 (MQ=255)
     tGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaaga                                                                               <  2:3922185/93‑1 (MQ=255)
      gggCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaagaa                                                                              <  1:4199455/93‑1 (MQ=255)
         cTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTc                                                                           <  2:540391/93‑1 (MQ=255)
            tATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGc                                                                        >  1:4118442/1‑93 (MQ=255)
             atgatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCa                                                                       >  1:3193086/1‑93 (MQ=255)
              tgatgCACAGCTGAAAGCCTGTAACGTGCTCGACTTCGATGAGCTGATGTTATGGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCaa                                                                      <  2:4039139/93‑1 (MQ=255)
              tgatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCaa                                                                      >  1:5277253/1‑93 (MQ=255)
               tatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAg                                                                     <  2:3334848/92‑1 (MQ=255)
               gatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCTGCAAg                                                                     >  1:6142755/1‑93 (MQ=255)
                atgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGGTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgc                                                                    >  2:5932178/1‑93 (MQ=255)
                 tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   >  1:1262602/1‑93 (MQ=255)
                 tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   <  2:5037723/93‑1 (MQ=255)
                  gCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  >  1:4090841/1‑93 (MQ=255)
                  gCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  >  2:6083033/1‑93 (MQ=255)
                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                >  2:3232656/1‑93 (MQ=255)
                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                >  2:2937492/1‑93 (MQ=255)
                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                >  2:2027038/1‑93 (MQ=255)
                    acacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTg                                                                <  1:5607163/93‑1 (MQ=255)
                     cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               <  2:928910/93‑1 (MQ=255)
                       ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  1:4381376/93‑1 (MQ=255)
                       ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             >  2:3424629/1‑93 (MQ=255)
                       ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  1:3973164/93‑1 (MQ=255)
                       ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  2:821340/93‑1 (MQ=255)
                       ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             >  2:5555570/1‑93 (MQ=255)
                         tGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGa                                                           <  1:497654/93‑1 (MQ=255)
                         tGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGa                                                           >  1:3094112/1‑93 (MQ=255)
                         tGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGa                                                           >  2:2347697/1‑93 (MQ=255)
                          gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  1:1358530/1‑93 (MQ=255)
                          gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          <  2:5592176/93‑1 (MQ=255)
                            aaGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACa                                                        >  2:2052971/1‑93 (MQ=255)
                             aGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaa                                                       >  2:575668/1‑93 (MQ=255)
                              gCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaa                                                      <  1:3786916/93‑1 (MQ=255)
                               ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  2:4012296/93‑1 (MQ=255)
                                cTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAt                                                    >  2:1335242/1‑93 (MQ=255)
                                  gTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTc                                                  <  1:3711616/93‑1 (MQ=255)
                                  gTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTc                                                  >  1:5864304/1‑93 (MQ=255)
                                    tgcGTTCTCGTCTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  2:5485725/91‑1 (MQ=255)
                                    aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  1:2648000/93‑1 (MQ=255)
                                    aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                >  1:1923024/1‑93 (MQ=255)
                                    aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  1:6110265/93‑1 (MQ=255)
                                    aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCAGCAAGCGCTGGCAGAACAAAATTCGc                                                >  2:1141461/1‑93 (MQ=255)
                                     aCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCt                                               <  2:4696122/93‑1 (MQ=255)
                                     aCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCt                                               >  1:868917/1‑93 (MQ=255)
                                           tcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgc                                         <  1:4268352/93‑1 (MQ=255)
                                           tcGACTTCGATGATCTGATTCTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgc                                         >  2:1753819/1‑93 (MQ=255)
                                            cGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgct                                        <  2:940273/93‑1 (MQ=255)
                                            cGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgct                                        <  1:1722849/93‑1 (MQ=255)
                                             gACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgctg                                       <  1:5377814/93‑1 (MQ=255)
                                                ttCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtg                                    >  1:1040369/1‑93 (MQ=255)
                                                ttCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtg                                    >  1:5217194/1‑93 (MQ=255)
                                                    atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                <  1:602050/93‑1 (MQ=255)
                                                    atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                >  2:6140449/1‑93 (MQ=255)
                                                    atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                >  2:5172391/1‑93 (MQ=255)
                                                    atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                <  1:4338102/93‑1 (MQ=255)
                                                      gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  1:2981461/1‑93 (MQ=255)
                                                      gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  2:3511890/1‑93 (MQ=255)
                                                       atCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGt                             >  1:3613419/1‑93 (MQ=255)
                                                         cTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAt                           <  1:5982553/93‑1 (MQ=255)
                                                         cTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAt                           <  1:1634385/93‑1 (MQ=255)
                                                         cTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAt                           <  2:2305832/93‑1 (MQ=255)
                                                          tGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATc                          >  1:1764367/1‑93 (MQ=255)
                                                          tGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATc                          <  2:4606036/93‑1 (MQ=255)
                                                           taTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         <  2:1707499/92‑1 (MQ=255)
                                                           gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATTAGTATCa                         >  2:1610262/1‑93 (MQ=255)
                                                            aTTTTATTGGCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        <  1:4569962/93‑1 (MQ=255)
                                                            aTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        >  1:1546500/1‑93 (MQ=255)
                                                            aTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        >  1:4829050/1‑93 (MQ=255)
                                                            aTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        >  1:2799857/1‑93 (MQ=255)
                                                            aTTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAg                        >  1:4226461/1‑93 (MQ=255)
                                                             ttttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAgg                       >  2:5864483/1‑93 (MQ=255)
                                                             ttttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAgg                       >  2:1504546/1‑93 (MQ=255)
                                                             ttttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAgg                       >  1:3304787/1‑93 (MQ=255)
                                                              tttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGa                      >  1:761973/1‑93 (MQ=255)
                                                               ttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGac                     <  2:797576/93‑1 (MQ=255)
                                                                 aTTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacac                   <  1:5806252/93‑1 (MQ=255)
                                                                 aTTGCCGACGTTGCTGCCGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacac                   >  2:4329616/1‑93 (MQ=255)
                                                                  ttGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacacc                  >  1:4702941/1‑93 (MQ=255)
                                                                   tGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacacca                 <  2:2181815/93‑1 (MQ=255)
                                                                    gCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaa                <  2:4399750/93‑1 (MQ=255)
                                                                    gCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaa                >  1:2205516/1‑93 (MQ=255)
                                                                      cGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacc              >  1:4630512/1‑92 (MQ=255)
                                                                      cGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaaca              <  2:1206904/93‑1 (MQ=255)
                                                                       gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  1:5623098/1‑93 (MQ=255)
                                                                       gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  2:2782292/1‑93 (MQ=255)
                                                                       gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  2:5709680/1‑93 (MQ=255)
                                                                        aCGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacc            >  1:2417310/1‑93 (MQ=255)
                                                                         cGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacca           <  2:3366333/93‑1 (MQ=255)
                                                                           ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         >  2:5311941/1‑93 (MQ=255)
                                                                           ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         >  2:1053323/1‑93 (MQ=255)
                                                                           ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         >  2:4851372/1‑93 (MQ=255)
                                                                           ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         >  1:4885856/1‑93 (MQ=255)
                                                                           ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         >  1:5260873/1‑93 (MQ=255)
                                                                           ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         >  1:3435967/1‑93 (MQ=255)
                                                                            tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        <  2:420532/93‑1 (MQ=255)
                                                                            tgctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcc        >  1:93875/1‑93 (MQ=255)
                                                                             actgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagcca       <  1:1160461/92‑1 (MQ=255)
                                                                              ctgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagccag      <  1:2516138/93‑1 (MQ=255)
                                                                                gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    <  1:5197222/93‑1 (MQ=255)
                                                                                 ctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAt   <  2:3685539/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958790‑3958964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA  >  NC_000913/3958784‑3958965
                                                                                             |                                                                                        
TCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                          <  SRR4302532.2464727/94‑1 (MQ=60)
GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                          >  SRR4302532.433714/1‑94 (MQ=60)
     TTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAAT                                                                                     >  SRR4302532.4178569/1‑94 (MQ=60)
       GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGA                                                                                   <  SRR4302532.3721769/94‑1 (MQ=60)
        TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAA                                                                                  <  SRR4302532.2884756/94‑1 (MQ=60)
         TATGGTCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                 <  SRR4302532.1554355/94‑1 (MQ=60)
         TATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                 <  SRR4302532.2524252/94‑1 (MQ=60)
         TATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTACTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                 <  SRR4302532.1179906/94‑1 (MQ=60)
          ATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGA                                                                                <  SRR4302532.5449997/94‑1 (MQ=60)
            GGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAG                                                                              <  SRR4302532.4340397/94‑1 (MQ=60)
             GGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGT                                                                             >  SRR4302532.5008305/1‑94 (MQ=60)
             GGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGT                                                                             <  SRR4302532.3161675/94‑1 (MQ=60)
               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                           >  SRR4302532.3292166/1‑94 (MQ=60)
                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTTCAACGCAATGAACAAGTCCG                                                                          >  SRR4302532.5772357/1‑94 (MQ=60)
                 GTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGC                                                                         >  SRR4302532.3402579/1‑94 (MQ=60)
                 GTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGC                                                                         >  SRR4302532.4256666/1‑94 (MQ=60)
                  TATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCA                                                                        >  SRR4302532.3299116/1‑94 (MQ=60)
                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAA                                                                       >  SRR4302532.5459245/1‑94 (MQ=60)
                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAA                                                                       >  SRR4302532.3602685/1‑94 (MQ=60)
                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAA                                                                       >  SRR4302532.4232739/1‑94 (MQ=60)
                    TGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCTGCAAG                                                                      >  SRR4302532.6354638/1‑94 (MQ=60)
                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                    >  SRR4302532.3772997/1‑94 (MQ=60)
                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                    >  SRR4302532.1304838/1‑94 (MQ=60)
                       TGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGC                                                                   >  SRR4302532.4228172/1‑94 (MQ=60)
                       TGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGC                                                                   >  SRR4302532.2983746/1‑94 (MQ=60)
                       TGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGC                                                                   <  SRR4302532.4979293/94‑1 (MQ=60)
                         CACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTG                                                                 >  SRR4302532.507389/1‑94 (MQ=60)
                          ACACGTGAAAGCCTGTAACGTTCTCGACTGCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGGCCGCAAGCGCTGG                                                                <  SRR4302532.1548825/94‑1 (MQ=60)
                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                >  SRR4302532.4857740/1‑94 (MQ=60)
                          ACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGG                                                                <  SRR4302532.5801791/94‑1 (MQ=60)
                           CACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGC                                                               >  SRR4302532.4352609/1‑94 (MQ=60)
                           CACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGC                                                               >  SRR4302532.4352610/1‑94 (MQ=60)
                             CCTGAAAGCCTGTAACGTTCTCGTCTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                             >  SRR4302532.1688219/1‑94 (MQ=60)
                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                             <  SRR4302532.4106994/94‑1 (MQ=60)
                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                             <  SRR4302532.4530210/94‑1 (MQ=60)
                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                             <  SRR4302532.4530211/94‑1 (MQ=60)
                              CTGAAAGCCTGTAACGTTCTCGACTTCGCTGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGA                                                            >  SRR4302532.1309162/1‑94 (MQ=60)
                              CTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGA                                                            <  SRR4302532.6228526/94‑1 (MQ=60)
                              CTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGA                                                            >  SRR4302532.3196678/1‑94 (MQ=60)
                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                           >  SRR4302532.2073655/1‑94 (MQ=60)
                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                           <  SRR4302532.515047/94‑1 (MQ=60)
                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                           >  SRR4302532.1404018/1‑94 (MQ=60)
                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                           >  SRR4302532.4449716/1‑94 (MQ=60)
                                    GCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAA                                                      <  SRR4302532.5978634/94‑1 (MQ=60)
                                    GCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAA                                                      <  SRR4302532.3914662/94‑1 (MQ=60)
                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                     <  SRR4302532.1874958/94‑1 (MQ=60)
                                      CTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATT                                                    >  SRR4302532.2857368/1‑94 (MQ=60)
                                      CTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATT                                                    >  SRR4302532.5459211/1‑94 (MQ=60)
                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                   <  SRR4302532.4668722/94‑1 (MQ=60)
                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                   >  SRR4302532.6067603/1‑94 (MQ=60)
                                       TGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTC                                                   >  SRR4302532.3180896/1‑94 (MQ=60)
                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                  >  SRR4302532.3679824/1‑94 (MQ=60)
                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                  <  SRR4302532.3836201/94‑1 (MQ=60)
                                         TAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGC                                                 >  SRR4302532.1986988/1‑94 (MQ=60)
                                         TAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGC                                                 >  SRR4302532.3901849/1‑94 (MQ=60)
                                         TAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGC                                                 >  SRR4302532.6172957/1‑94 (MQ=60)
                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                >  SRR4302532.898686/1‑94 (MQ=60)
                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                >  SRR4302532.898685/1‑94 (MQ=60)
                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                <  SRR4302532.6321170/94‑1 (MQ=60)
                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                <  SRR4302532.2736825/94‑1 (MQ=60)
                                            CGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAT                                              <  SRR4302532.2606075/94‑1 (MQ=60)
                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCTCTGGCAGAACAAAATTCGCTATC                                             <  SRR4302532.4385020/94‑1 (MQ=60)
                                              TTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCT                                            <  SRR4302532.1712996/94‑1 (MQ=60)
                                              TTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCACAACAAACTTCGCTATCG                                            >  SRR4302532.1226552/1‑94 (MQ=60)
                                               TCTCGACTTCGATGATTTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTG                                           <  SRR4302532.62205/94‑1 (MQ=60)
                                                 TCGACTTCGATGATCTTATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                         <  SRR4302532.1912512/94‑1 (MQ=60)
                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                         <  SRR4302532.4412061/94‑1 (MQ=60)
                                                 TCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCT                                         >  SRR4302532.2338277/1‑94 (MQ=60)
                                                  CGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTG                                        <  SRR4302532.1780874/94‑1 (MQ=60)
                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                       <  SRR4302532.5564097/94‑1 (MQ=60)
                                                    ACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGT                                      <  SRR4302532.5025389/94‑1 (MQ=60)
                                                    ACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGT                                      >  SRR4302532.3432754/1‑94 (MQ=60)
                                                    ACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGCCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGT                                      <  SRR4302532.2355526/94‑1 (MQ=60)
                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                     >  SRR4302532.4334548/1‑94 (MQ=60)
                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                     >  SRR4302532.5397120/1‑94 (MQ=60)
                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                     >  SRR4302532.1075453/1‑94 (MQ=60)
                                                      TTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGG                                    <  SRR4302532.1756175/94‑1 (MQ=60)
                                                      TTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGG                                    <  SRR4302532.903249/94‑1 (MQ=60)
                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGTT                                  <  SRR4302532.4729484/94‑1 (MQ=60)
                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAAAGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGGT                                  >  SRR4302532.4406006/1‑94 (MQ=60)
                                                         GATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                 <  SRR4302532.182012/94‑1 (MQ=60)
                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                <  SRR4302532.4484633/94‑1 (MQ=60)
                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                <  SRR4302532.6284090/94‑1 (MQ=60)
                                                          ATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGA                                <  SRR4302532.623079/94‑1 (MQ=60)
                                                          AGGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGATGTCCGCAAGCGCTGGCAGAACAAAATTCGCGATCTGCTGGTGGATGA                                >  SRR4302532.5691224/1‑94 (MQ=60)
                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                               <  SRR4302532.668986/94‑1 (MQ=60)
                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                               >  SRR4302532.3080065/1‑94 (MQ=60)
                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                               <  SRR4302532.5395716/94‑1 (MQ=60)
                                                            GATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGT                              >  SRR4302532.3734252/1‑94 (MQ=60)
                                                             ATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTA                             >  SRR4302532.1153998/1‑94 (MQ=60)
                                                               CTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATC                           >  SRR4302532.1823509/1‑94 (MQ=60)
                                                               CTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATC                           <  SRR4302532.6189265/94‑1 (MQ=60)
                                                               CTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATC                           <  SRR4302532.1689700/94‑1 (MQ=60)
                                                               CTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATC                           >  SRR4302532.5370408/1‑94 (MQ=60)
                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                          >  SRR4302532.664598/1‑94 (MQ=60)
                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                          <  SRR4302532.5463107/94‑1 (MQ=60)
                                                                 TATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                         <  SRR4302532.1195837/94‑1 (MQ=60)
                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                         >  SRR4302532.4995295/1‑94 (MQ=60)
                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                         >  SRR4302532.2893679/1‑94 (MQ=60)
                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                         >  SRR4302532.1598754/1‑94 (MQ=60)
                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                         <  SRR4302532.3370789/94‑1 (MQ=60)
                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                         >  SRR4302532.4368333/1‑94 (MQ=60)
                                                                  ATTTTATTGGCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                        <  SRR4302532.4725832/94‑1 (MQ=60)
                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                        >  SRR4302532.4453214/1‑94 (MQ=60)
                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                        >  SRR4302532.3414821/1‑94 (MQ=60)
                                                                   TTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGA                       >  SRR4302532.788225/1‑94 (MQ=60)
                                                                   TTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGA                       <  SRR4302532.6352671/94‑1 (MQ=60)
                                                                       ATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACC                   >  SRR4302532.4863379/1‑94 (MQ=60)
                                                                       ATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACC                   <  SRR4302532.6007996/94‑1 (MQ=60)
                                                                       ATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACC                   >  SRR4302532.1469823/1‑94 (MQ=60)
                                                                        TTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGGATCAGGACACCA                  >  SRR4302532.3896361/1‑94 (MQ=60)
                                                                         TGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAA                 >  SRR4302532.2278865/1‑94 (MQ=60)
                                                                          GCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAAC                <  SRR4302532.6028451/94‑1 (MQ=60)
                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACC               >  SRR4302532.4788399/1‑94 (MQ=60)
                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC              >  SRR4302532.5818220/1‑94 (MQ=60)
                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC              <  SRR4302532.5415802/94‑1 (MQ=60)
                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCACAAGAGCTGGCAGAACAAAATTAGCTATCTGCTGGTGGATGAGagacacgacaccaccac              >  SRR4302532.618715/1‑77 (MQ=60)
                                                                             GACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACC             >  SRR4302532.4112464/1‑94 (MQ=60)
                                                                             GACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACC             >  SRR4302532.2497688/1‑94 (MQ=60)
                                                                               CGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAG           >  SRR4302532.1217496/1‑94 (MQ=60)
                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC          >  SRR4302532.5207466/1‑94 (MQ=60)
                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC          >  SRR4302532.3551098/1‑94 (MQ=60)
                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC          >  SRR4302532.5053801/1‑94 (MQ=60)
                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC          >  SRR4302532.5442292/1‑94 (MQ=60)
                                                                                 TTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCC         >  SRR4302532.97152/1‑94 (MQ=60)
                                                                                   ACTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAG       <  SRR4302532.1199230/94‑1 (MQ=60)
                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT      <  SRR4302532.2600095/94‑1 (MQ=60)
                                                                                    CTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGT      >  SRR4302532.4797118/1‑94 (MQ=60)
                                                                                      GCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAT    <  SRR4302532.5811108/94‑1 (MQ=60)
                                                                                      GCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAT    <  SRR4302532.5376380/94‑1 (MQ=60)
                                                                                      GCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAT    <  SRR4302532.4541012/94‑1 (MQ=60)
                                                                                       CTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG   <  SRR4302532.3922790/94‑1 (MQ=60)
                                                                                       CTGCAACACAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG   >  SRR4302532.2417962/1‑94 (MQ=60)
                                                                                        TGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA  >  SRR4302532.199926/1‑94 (MQ=60)
                                                                                        TGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA  <  SRR4302532.5999272/94‑1 (MQ=60)
                                                                                        TGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA  >  SRR4302532.5639408/1‑94 (MQ=60)
                                                                                        TGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA  >  SRR4302532.3826651/1‑94 (MQ=60)
                                                                                             |                                                                                        
GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA  >  NC_000913/3958784‑3958965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑