Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,979,546 Δ1 bp coding (1047/1386 nt) yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,979,5450G.100.0% 1102.0 / NA 234coding (1046/1386 nt)yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (125/109);  total (125/109)

Only 100 of 249 total aligned reads displayed.

TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633
                                                                                            |                                                                                        
ttACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTg                                                                                          >  2:5130881/1‑93 (MQ=255)
 tACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTga                                                                                         >  2:2012404/1‑92 (MQ=255)
  aCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaa                                                                                        <  2:1287056/93‑3 (MQ=255)
   ccGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaac                                                                                       <  2:3852623/93‑4 (MQ=255)
   ccGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTAATGCTATTCTGCTAATTGGCTCATGCCTgaac                                                                                       <  1:3417084/93‑4 (MQ=255)
    cggcggCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaact                                                                                      <  2:580992/93‑5 (MQ=255)
     ggcggcAATTGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                                    <  2:1641855/93‑1 (MQ=255)
     ggcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                                    <  1:945929/93‑1 (MQ=255)
     ggcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                                    <  2:1737160/93‑1 (MQ=255)
     ggcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                                    <  1:4045766/93‑1 (MQ=255)
       cggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAca                                                                                  <  2:829135/93‑1 (MQ=255)
        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                                 <  1:4712717/93‑1 (MQ=255)
        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                                 >  1:1875079/1‑93 (MQ=255)
        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                                 <  2:5206169/93‑1 (MQ=255)
          cAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatca                                                                               <  2:555402/93‑1 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              <  1:4027532/93‑1 (MQ=255)
           aaTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatcat                                                                              >  1:3971667/1‑93 (MQ=255)
            aTGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                             >  1:4179509/1‑93 (MQ=255)
             tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTc                                                                            >  2:2962337/1‑93 (MQ=255)
               gCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTccc                                                                          >  2:2501371/1‑93 (MQ=255)
                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         <  2:3107343/93‑1 (MQ=255)
                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         >  2:4769809/1‑93 (MQ=255)
                 gAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCaa                                                                        >  2:943689/1‑93 (MQ=255)
                  aaaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAt                                                                       <  1:99795/93‑1 (MQ=255)
                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  1:128481/93‑1 (MQ=255)
                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:5708690/93‑1 (MQ=255)
                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  1:5217507/93‑1 (MQ=255)
                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTCCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:47098/93‑1 (MQ=255)
                     gTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCg                                                                    <  2:5431761/93‑1 (MQ=255)
                      tttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGc                                                                   >  1:3961903/1‑93 (MQ=255)
                       ttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCa                                                                  <  2:3939317/93‑1 (MQ=255)
                        tCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAg                                                                 >  1:300563/1‑93 (MQ=255)
                          ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCg                                                               >  2:2731750/1‑93 (MQ=255)
                          ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCg                                                               <  2:762137/93‑1 (MQ=255)
                          ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCg                                                               >  1:5371014/1‑93 (MQ=255)
                           cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              <  1:3469853/93‑1 (MQ=255)
                           cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              <  1:728123/93‑1 (MQ=255)
                           cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              <  2:5278048/93‑1 (MQ=255)
                             tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgt                                                            <  2:2202253/93‑1 (MQ=255)
                                 ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        >  2:1452120/1‑93 (MQ=255)
                                  gCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                                       >  2:3486929/1‑93 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:6087631/93‑1 (MQ=255)
                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     <  1:1390659/93‑1 (MQ=255)
                                      aCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtcta                                                   >  1:2904961/1‑93 (MQ=255)
                                       ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCCGCGAGTGTTTgtctac                                                  >  1:2293256/1‑93 (MQ=255)
                                       ccGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctac                                                  >  2:2864375/1‑93 (MQ=255)
                                         ggTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgt                                                <  2:2653203/93‑1 (MQ=255)
                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  2:2535390/1‑93 (MQ=255)
                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  1:3844860/1‑93 (MQ=255)
                                            tGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                             <  1:5415912/93‑1 (MQ=255)
                                              cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                           <  2:5978765/93‑1 (MQ=255)
                                              cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                           <  2:4467621/93‑1 (MQ=255)
                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          <  2:5743046/93‑1 (MQ=255)
                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  1:1787651/1‑93 (MQ=255)
                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTAATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  1:5691793/1‑93 (MQ=255)
                                                ggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTCCAGt                                         >  2:3422731/1‑93 (MQ=255)
                                                 gtgtGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTg                                        >  1:3719266/1‑93 (MQ=255)
                                                  tttgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGc                                       >  2:5457571/3‑93 (MQ=255)
                                                  tgtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGc                                       <  2:3645381/93‑1 (MQ=255)
                                                   gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                                      >  2:1120931/1‑93 (MQ=255)
                                                    tgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     <  1:1584754/93‑1 (MQ=255)
                                                    tgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     <  2:5070985/93‑1 (MQ=255)
                                                    tgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     >  2:3677184/1‑93 (MQ=255)
                                                    gggCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     <  1:286200/92‑1 (MQ=255)
                                                      gCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGc                                   <  1:597366/93‑1 (MQ=255)
                                                        aGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                                 >  1:5097153/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  2:78118/1‑93 (MQ=255)
                                                         gTATCTATTGCTATTCTGCTAATTGGCTCATGACT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCGGCGTg                                >  1:3864202/1‑93 (MQ=255)
                                                          tATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGc                               >  1:4528545/1‑93 (MQ=255)
                                                           aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              <  1:2812112/93‑1 (MQ=255)
                                                             cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            <  1:32843/93‑1 (MQ=255)
                                                             cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            <  2:3912414/93‑1 (MQ=255)
                                                             cTATTGCTATGCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCATCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            <  1:32839/93‑1 (MQ=255)
                                                                ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         <  1:6024236/93‑1 (MQ=255)
                                                                ttGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgg                         >  1:3095674/1‑93 (MQ=255)
                                                                 tGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        >  1:4982895/1‑93 (MQ=255)
                                                                 tGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        <  1:1104394/93‑1 (MQ=255)
                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      >  2:6098949/1‑93 (MQ=255)
                                                                    tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTCCGTCTACAGTGCCAGCGTGCTTCCGGGGAt                     <  1:2433417/93‑1 (MQ=255)
                                                                    tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt                     >  2:4381088/1‑93 (MQ=255)
                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   <  2:2352688/93‑1 (MQ=255)
                                                                         tGCTAATTGGCTCATGCCT‑GAACTCCATCATCGCAAATCCGCAGCGTGTGTTTGTCTGCGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                <  1:3559782/93‑1 (MQ=255)
                                                                         tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                <  2:4910483/93‑1 (MQ=255)
                                                                          gCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGcc               >  1:352889/1‑93 (MQ=255)
                                                                           cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              <  2:4116433/93‑1 (MQ=255)
                                                                              attggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATgg           <  1:1658324/89‑1 (MQ=255)
                                                                               ttggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGt          >  1:2239603/4‑93 (MQ=255)
                                                                                tggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGtt         <  1:3707059/91‑1 (MQ=255)
                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        >  1:5236310/2‑93 (MQ=255)
                                                                                  gCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtg       >  1:1935661/1‑93 (MQ=255)
                                                                                  gCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtg       >  2:1584507/1‑93 (MQ=255)
                                                                                   cTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtgt      >  1:5772454/1‑93 (MQ=255)
                                                                                    tCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGCTTGTCTCCGTCTACACTGACAGAGTGCTTCCGGGGATGGTCCCATGGTTtgtg     >  2:3215724/1‑93 (MQ=255)
                                                                                     cATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa    >  1:4289653/1‑93 (MQ=255)
                                                                                     cATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa    <  1:1927099/93‑1 (MQ=255)
                                                                                      aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  2:4295476/1‑93 (MQ=255)
                                                                                      aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   <  2:2277965/93‑1 (MQ=255)
                                                                                       tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  <  2:3821158/93‑1 (MQ=255)
                                                                                       tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  >  2:2460015/1‑93 (MQ=255)
                                                                                       tGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGata  >  2:4618831/1‑93 (MQ=255)
                                                                                            |                                                                                        
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑G‑CTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979454‑3979639
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TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCTGA                                                                                               >  SRR4302532.1479217/1‑94 (MQ=60)
 TACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCTGAA                                                                                              >  SRR4302532.1249449/1‑94 (MQ=60)
  ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAAC                                                                                            <  SRR4302532.6355660/94‑1 (MQ=60)
  ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAAC                                                                                            <  SRR4302532.6268042/94‑1 (MQ=60)
   CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTAATGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACT                                                                                           <  SRR4302532.3531545/94‑1 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTAC                                                                                         <  SRR4302532.978308/94‑1 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTAC                                                                                         <  SRR4302532.5730664/94‑1 (MQ=60)
     GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTAC                                                                                         <  SRR4302532.4181967/94‑1 (MQ=60)
       CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACAT                                                                                       >  SRR4302532.1937186/1‑94 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAGTTGG‑CTCATGCCT‑GAACTACATC                                                                                      >  SRR4302532.3603737/1‑94 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATC                                                                                      <  SRR4302532.5501633/94‑1 (MQ=60)
        GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATC                                                                                      <  SRR4302532.4873517/94‑1 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCAT                                                                                    <  SRR4302532.2196956/94‑1 (MQ=60)
          CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCAT                                                                                    >  SRR4302532.4105453/1‑94 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATT                                                                                   <  SRR4302532.4163256/94‑1 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATT                                                                                   >  SRR4302532.4319700/1‑94 (MQ=60)
           AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATT                                                                                   >  SRR4302532.2654066/1‑94 (MQ=60)
            ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTC                                                                                  >  SRR4302532.306075/1‑94 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAAT                                                                             <  SRR4302532.4274597/94‑1 (MQ=60)
                 GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAAT                                                                             >  SRR4302532.1867644/1‑94 (MQ=60)
                  AAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATC                                                                            <  SRR4302532.103260/94‑1 (MQ=60)
                   AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302532.132820/94‑1 (MQ=60)
                   AAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCC                                                                           <  SRR4302532.5397445/94‑1 (MQ=60)
                     GTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGC                                                                         >  SRR4302532.4095388/1‑94 (MQ=60)
                       TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       >  SRR4302532.310962/1‑94 (MQ=60)
                       TTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAG                                                                       <  SRR4302532.6162010/94‑1 (MQ=60)
                        TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      <  SRR4302532.3748311/94‑1 (MQ=60)
                        TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      >  SRR4302532.5437322/1‑94 (MQ=60)
                        TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                      <  SRR4302532.5404704/94‑1 (MQ=60)
                         CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     >  SRR4302532.3892704/1‑94 (MQ=60)
                         CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                     >  SRR4302532.5557117/1‑94 (MQ=60)
                          CCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACCACATCATTCCCAATCCGCAGCGT                                                                    >  SRR4302532.3613464/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302532.4778565/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302532.1957474/94‑1 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302532.537375/1‑94 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302532.3586059/94‑1 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   <  SRR4302532.752933/94‑1 (MQ=60)
                           CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                   >  SRR4302532.3989807/1‑94 (MQ=60)
                           CGTCACGGCGGCCCGGTTGCGGGTGTGGCAGTATCGATTGCTATTCTGCTAATTGG‑CTCATGCC‑GGAACTACATGATTCCCAATCCGCAGaggg                                                                   <  SRR4302532.1313645/94‑5 (MQ=60)
                            GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  <  SRR4302532.4164866/94‑1 (MQ=60)
                            GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGT                                                                  >  SRR4302532.3206037/1‑94 (MQ=60)
                            GTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGG                                                                  >  SRR4302532.1467735/1‑94 (MQ=60)
                             TCACTGCGTAGCGGTTGTGGGTGTGGCAGTATCTATTGCTATTCTGGTAATTGG‑GGCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTG                                                                 <  SRR4302532.6233616/94‑1 (MQ=53)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              >  SRR4302532.3325893/1‑94 (MQ=60)
                                CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                              <  SRR4302532.2418875/94‑1 (MQ=60)
                                   CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           >  SRR4302532.231169/1‑94 (MQ=60)
                                   CGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTC                                                           >  SRR4302532.5595545/1‑94 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302532.6297813/94‑1 (MQ=60)
                                    GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                          <  SRR4302532.1437287/94‑1 (MQ=60)
                                     TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         >  SRR4302532.3001870/1‑94 (MQ=60)
                                     TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                         >  SRR4302532.3489362/1‑94 (MQ=60)
                                     TACCGGTTGCGGGGGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTTCCAATCCGCAGCGTGTGTTTGTCTA                                                         >  SRR4302532.3489361/1‑94 (MQ=60)
                                      ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCCGCGAGTGTTTGTCTAC                                                        >  SRR4302532.2369375/1‑94 (MQ=60)
                                      ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        <  SRR4302532.309525/94‑1 (MQ=60)
                                      ACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTAC                                                        <  SRR4302532.2934925/94‑1 (MQ=60)
                                        CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                      <  SRR4302532.4101908/94‑1 (MQ=60)
                                         GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302532.1984609/1‑94 (MQ=60)
                                         GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302532.3974679/1‑94 (MQ=60)
                                         GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                     >  SRR4302532.1778304/1‑94 (MQ=60)
                                            TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                  <  SRR4302532.5603593/94‑1 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302532.1847397/1‑94 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                <  SRR4302532.5372188/94‑1 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302532.4259745/1‑94 (MQ=60)
                                              CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTAATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                                >  SRR4302532.5890399/1‑94 (MQ=60)
                                               GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                               <  SRR4302532.816831/94‑1 (MQ=60)
                                                GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCTGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              <  SRR4302532.5205718/94‑1 (MQ=60)
                                                GGTGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTG                                              >  SRR4302532.3844116/1‑94 (MQ=60)
                                                  TGTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCC                                            >  SRR4302532.1343168/1‑94 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           >  SRR4302532.392628/1‑94 (MQ=60)
                                                   GTGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                           >  SRR4302532.514836/1‑94 (MQ=60)
                                                    TGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                          <  SRR4302532.296080/94‑1 (MQ=60)
                                                    TGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                          <  SRR4302532.1638392/94‑1 (MQ=60)
                                                    GGGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                          <  SRR4302532.296081/94‑1 (MQ=60)
                                                     GGCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGC                                         >  SRR4302532.3568555/1‑94 (MQ=60)
                                                      GCAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                                        <  SRR4302532.618213/94‑1 (MQ=60)
                                                      GCAGTATCTATTGCTATTCTGCTAATT‑GTCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCG                                        <  SRR4302532.1998902/94‑1 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       >  SRR4302532.5272415/1‑94 (MQ=60)
                                                       CAGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                       <  SRR4302532.4596421/94‑1 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                      >  SRR4302532.1010004/1‑94 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGCGCCAGCGTG                                      <  SRR4302532.4338840/94‑1 (MQ=60)
                                                        AGTATCTATTGCTATTCTGCTAATTGG‑CTCATGACT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCGGCGTG                                      >  SRR4302532.3994629/1‑94 (MQ=60)
                                                         GTATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                     >  SRR4302532.4683108/1‑94 (MQ=60)
                                                           ATCTATTGCTGTTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   <  SRR4302532.2344774/94‑1 (MQ=60)
                                                           ATCTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                   <  SRR4302532.2906255/94‑1 (MQ=60)
                                                             CTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302532.34081/94‑1 (MQ=60)
                                                             CTATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302532.34080/94‑1 (MQ=60)
                                                             CTATTGCTATGCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCATCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                 <  SRR4302532.34077/94‑1 (MQ=60)
                                                              TATTTCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                <  SRR4302532.1803722/94‑1 (MQ=60)
                                                              TATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                                <  SRR4302532.6085520/94‑1 (MQ=60)
                                                              TATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGGGCTTCCG                                >  SRR4302532.4153138/1‑94 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302532.3838933/1‑94 (MQ=60)
                                                               ATTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                               >  SRR4302532.3198305/1‑94 (MQ=60)
                                                                ctgcttttctgcgaattgG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCATCGTGTGTGTGTCTACGTCTACAGTGCCAGTGCGCTTCCGGG                              >  SRR4302532.5654141/19‑94 (MQ=60)
                                                                TTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              <  SRR4302532.6232295/94‑1 (MQ=60)
                                                                TTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              >  SRR4302532.4535491/1‑94 (MQ=60)
                                                                TTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              >  SRR4302532.5153707/1‑94 (MQ=60)
                                                                TTGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                              <  SRR4302532.4051241/94‑1 (MQ=60)
                                                                 TGCTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                             <  SRR4302532.1141312/94‑1 (MQ=60)
                                                                   CTATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                           >  SRR4302532.5144717/1‑94 (MQ=60)
                                                                    TATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTCCGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          <  SRR4302532.2514307/94‑1 (MQ=60)
                                                                    TATTCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                          <  SRR4302532.5902561/94‑1 (MQ=60)
                                                                       TCTGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGcgcggggg                       >  SRR4302532.549118/1‑86 (MQ=60)
                                                                         TGCTAATTGG‑CTCATGCCT‑GAACTCCATCATCGCAAATCCGCAGCGTGTGTTTGTCTGCGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     <  SRR4302532.3678894/94‑1 (MQ=60)
                                                                         TGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     >  SRR4302532.4064741/1‑94 (MQ=60)
                                                                         TGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     >  SRR4302532.365103/1‑94 (MQ=60)
                                                                         TGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     >  SRR4302532.3167238/1‑94 (MQ=60)
                                                                         TGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                     >  SRR4302532.1685204/1‑94 (MQ=60)
                                                                         TGCTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGAC                     >  SRR4302532.365104/1‑94 (MQ=60)
                                                                           CTAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                   >  SRR4302532.6232120/1‑94 (MQ=60)
                                                                            TAATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGCTGCCATG                  >  SRR4302532.4433610/1‑94 (MQ=60)
                                                                             AATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                 >  SRR4302532.2975658/1‑94 (MQ=60)
                                                                              ATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                <  SRR4302532.2385749/94‑1 (MQ=60)
                                                                              ATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                >  SRR4302532.2313919/1‑94 (MQ=60)
                                                                              ATTGG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGT                <  SRR4302532.1714531/94‑1 (MQ=60)
                                                                               ttgG‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTT               >  SRR4302532.4120441/4‑94 (MQ=60)
                                                                               TG‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              >  SRR4302532.5416877/1‑94 (MQ=60)
                                                                               TG‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT              <  SRR4302532.3831511/94‑1 (MQ=60)
                                                                                G‑G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTG             >  SRR4302532.2000071/1‑94 (MQ=60)
                                                                                G‑G‑CTCATGCCT‑GAAATACCTCATTCCCAATCAGGAGCGTGGGTTTGTGTACGTCTACAGTGACTGCAAGCTTCCGGGGATGGTGCCATGGTTTG             >  SRR4302532.3708577/1‑94 (MQ=26)
                                                                                  G‑CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGT            >  SRR4302532.5973297/1‑94 (MQ=60)
                                                                                     TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          >  SRR4302532.4434389/1‑94 (MQ=60)
                                                                                     TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA          >  SRR4302532.3806280/1‑94 (MQ=60)
                                                                                      CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         <  SRR4302532.1991223/94‑1 (MQ=60)
                                                                                      CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT         >  SRR4302532.817459/1‑94 (MQ=60)
                                                                                        TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT       >  SRR4302532.4413223/1‑94 (MQ=60)
                                                                                          CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG     >  SRR4302532.6086153/1‑94 (MQ=60)
                                                                                          CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG     <  SRR4302532.3364408/94‑1 (MQ=60)
                                                                                            ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    <  SRR4302532.528351/92‑1 (MQ=60)
                                                                                            ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA    <  SRR4302532.528350/92‑1 (MQ=60)
                                                                                              GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  <  SRR4302532.4585319/94‑1 (MQ=60)
                                                                                              GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  SRR4302532.2456855/1‑94 (MQ=60)
                                                                                              |                                                                                             
TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑G‑CTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGATA  >  NC_000913/3979454‑3979639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑