Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,546	Δ1 bp	coding (1047/1386 nt)	yifK →	putative amino acid/amine transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,545	0	G	.	100.0%	1155.8 / NA	244	coding (1046/1386 nt)	yifK	putative amino acid/amine transport protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (135/109); total (135/109)

Only 100 of 252 total aligned reads displayed.

TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633

ttACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTg                                                                                          <  2:2066186/93‑1 (MQ=255)

  aCCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTgaa                                                                                        <  1:5362667/93‑3 (MQ=255)

     ggcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTa                                                                                    <  1:1633863/93‑1 (MQ=255)

      gcggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAc                                                                                   >  1:2259241/1‑93 (MQ=255)

        ggcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcat                                                                                 <  1:1135520/93‑1 (MQ=255)

         gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                                <  1:1561080/93‑1 (MQ=255)

         gcAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAcatc                                                                                >  2:1019430/1‑93 (MQ=255)

            aTGGCGAAAGTTTCCCGTCACGTCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAtt                                                                             <  1:3599355/93‑1 (MQ=255)

             tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTc                                                                            <  1:1098366/93‑1 (MQ=255)

             tGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTc                                                                            >  2:1720756/1‑93 (MQ=255)

              ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                           >  1:1362238/1‑93 (MQ=255)

              ggCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTcc                                                                           >  2:4654314/1‑93 (MQ=255)

                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         >  2:668864/1‑93 (MQ=255)

                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         <  2:5310823/93‑1 (MQ=255)

                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         <  2:3463189/93‑1 (MQ=255)

                cGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCa                                                                         >  2:3166754/1‑93 (MQ=255)

                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:1250777/93‑1 (MQ=255)

                   aaGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATc                                                                      <  2:1215810/93‑1 (MQ=255)

                     gTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCg                                                                    <  2:3097512/93‑1 (MQ=255)

                      tttCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGc                                                                   >  2:149402/1‑93 (MQ=255)

                         cccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGc                                                                <  2:5048096/93‑1 (MQ=255)

                         cccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGc                                                                <  1:6119089/93‑1 (MQ=255)

                          ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCg                                                               >  2:222345/1‑93 (MQ=255)

                          ccGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCg                                                               >  2:3656315/1‑93 (MQ=255)

                           cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              <  2:5226826/93‑1 (MQ=255)

                           cGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgt                                                              <  2:4100331/93‑1 (MQ=255)

                             tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgt                                                            >  2:436605/1‑93 (MQ=255)

                             tCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgt                                                            <  1:5509957/93‑1 (MQ=255)

                              cACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtg                                                           >  2:4206463/1‑93 (MQ=255)

                               aCGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCgtgtgt                                                          <  1:591701/93‑1 (MQ=255)

                                 ggCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGttt                                                        <  1:2518629/93‑1 (MQ=255)

                                  gCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTg                                                       <  2:6315670/93‑1 (MQ=255)

                                   cGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgt                                                      >  2:3055814/1‑93 (MQ=255)

                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     >  2:3964850/1‑93 (MQ=255)

                                    gTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtc                                                     >  2:45213/1‑93 (MQ=255)

                                     tACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtct                                                    >  2:4937095/1‑93 (MQ=255)

                                        cGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacg                                                 <  1:2575212/93‑1 (MQ=255)

                                        cGGTTGCGGGTGTGGCAGTATCTATTGCTATCCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacg                                                 <  2:3821825/93‑1 (MQ=255)

                                         ggTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgt                                                >  1:5668049/1‑93 (MQ=255)

                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtt                                               >  2:5482512/1‑92 (MQ=255)

                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               <  2:2304927/93‑1 (MQ=255)

                                          gTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtc                                               >  2:1429436/1‑93 (MQ=255)

                                           ttGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtct                                              >  2:2928733/1‑93 (MQ=255)

                                            tGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                             >  2:3454836/1‑93 (MQ=255)

                                            tGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                             <  2:2533887/93‑1 (MQ=255)

                                            tGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtcta                                             <  2:4695677/93‑1 (MQ=255)

                                             gCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTgtctacgtctac                                            <  1:583913/93‑1 (MQ=255)

                                              cGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACa                                           >  2:3249555/1‑93 (MQ=255)

                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          >  2:3363733/1‑93 (MQ=255)

                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          <  2:5422572/93‑1 (MQ=255)

                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          <  1:3812832/93‑1 (MQ=255)

                                               gggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAg                                          <  2:1006777/93‑1 (MQ=255)

                                                ggTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGt                                         >  2:2872824/1‑93 (MQ=255)

                                                   gtgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGcc                                      >  2:3863708/1‑93 (MQ=255)

                                                    tgGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCa                                     <  1:6248437/93‑1 (MQ=255)

                                                     ggCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                                    >  2:4468793/1‑93 (MQ=255)

                                                     ggCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                                    >  1:5906746/1‑93 (MQ=255)

                                                     ggCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAg                                    >  2:1833933/1‑93 (MQ=255)

                                                        aGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGt                                 <  1:3538675/93‑1 (MQ=255)

                                                         gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                <  1:1054108/93‑1 (MQ=255)

                                                         gTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTg                                >  1:4941112/1‑93 (MQ=255)

                                                           aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              <  2:2172568/93‑1 (MQ=255)

                                                           aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              <  1:6624881/93‑1 (MQ=255)

                                                           aTCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCt                              <  1:764059/93‑1 (MQ=255)

                                                            tCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTTTACAGTGCCAGCATGCtt                             >  2:1927867/1‑93 (MQ=255)

                                                             cTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTc                            <  2:6493663/93‑1 (MQ=255)

                                                              tATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTcc                           >  1:3137784/1‑93 (MQ=255)

                                                               aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          >  2:5185104/1‑93 (MQ=255)

                                                               aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          >  1:3279915/1‑93 (MQ=255)

                                                               aTTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCg                          <  1:5584478/93‑1 (MQ=255)

                                                                 tGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCggg                        <  1:3247223/93‑1 (MQ=255)

                                                                  gCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgggg                       >  2:2403021/1‑93 (MQ=255)

                                                                  gCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCgggg                       >  1:3406468/1‑93 (MQ=255)

                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      <  1:2201136/93‑1 (MQ=255)

                                                                   cTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGa                      >  1:231130/1‑93 (MQ=255)

                                                                    tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt                     <  2:6084630/93‑1 (MQ=255)

                                                                    tATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAt                     <  2:5691273/93‑1 (MQ=255)

                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  1:2403771/1‑93 (MQ=255)

                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  2:1570160/1‑93 (MQ=255)

                                                                      ttCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATgg                   >  2:456611/1‑93 (MQ=255)

                                                                       tCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  >  1:4869491/1‑93 (MQ=255)

                                                                       tCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGt                  <  1:4597673/93‑1 (MQ=255)

                                                                         tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                >  2:5921575/1‑93 (MQ=255)

                                                                         tGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGc                <  1:1904948/93‑1 (MQ=255)

                                                                          gCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGcc               >  1:2791767/1‑93 (MQ=255)

                                                                           cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              >  1:6557826/1‑93 (MQ=255)

                                                                           cTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCa              <  2:3334187/93‑1 (MQ=255)

                                                                            tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             >  1:4660559/1‑93 (MQ=255)

                                                                            tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             >  1:2969151/1‑93 (MQ=255)

                                                                            tAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAt             <  2:5523276/93‑1 (MQ=255)

                                                                              attggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATgg           >  1:4328527/5‑93 (MQ=255)

                                                                              attggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATgg           >  1:1178182/5‑93 (MQ=255)

                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        >  1:4884250/2‑93 (MQ=255)

                                                                                 ggCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGttt        >  2:5827149/2‑93 (MQ=255)

                                                                                   cTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtgt      >  1:798049/1‑93 (MQ=255)

                                                                                    tCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtgtg     <  1:5031851/93‑1 (MQ=255)

                                                                                    tCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTtgtg     >  2:3608873/1‑93 (MQ=255)

                                                                                     cATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGa    <  1:5775526/93‑1 (MQ=255)

                                                                                      aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  1:3683351/1‑93 (MQ=255)

                                                                                      aTGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGat   >  2:133859/1‑93 (MQ=255)

TTACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA  >  NC_000913/3979454‑3979633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  >  NC_000913/3979456‑3979638

ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAAC                                                                                           <  SRR4302547.5473283/94‑1 (MQ=60)

 CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCTGGAct                                                                                           >  SRR4302547.453698/1‑92 (MQ=60)

 CCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACT                                                                                          >  SRR4302547.5093764/1‑94 (MQ=60)

   GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAC                                                                                        >  SRR4302547.2304879/1‑94 (MQ=60)

   GGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTAC                                                                                        <  SRR4302547.1666186/94‑1 (MQ=60)

    GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACA                                                                                       >  SRR4302547.234512/1‑94 (MQ=60)

     CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                      >  SRR4302547.585545/1‑94 (MQ=60)

     CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                      >  SRR4302547.3387897/1‑94 (MQ=60)

     CGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACAT                                                                                      >  SRR4302547.128395/1‑94 (MQ=60)

      GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                     >  SRR4302547.5638127/1‑94 (MQ=60)

      GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                     <  SRR4302547.2052336/94‑1 (MQ=60)

      GGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATC                                                                                     <  SRR4302547.1158451/94‑1 (MQ=60)

       GCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCA                                                                                    <  SRR4302547.1592176/94‑1 (MQ=60)

        CAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCAT                                                                                   >  SRR4302547.5034242/1‑94 (MQ=60)

         AATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATT                                                                                  <  SRR4302547.5839672/94‑1 (MQ=60)

          ATGGCGAAAGTTTCCCGTCACGTCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                 <  SRR4302547.3672746/94‑1 (MQ=60)

          ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                 >  SRR4302547.6172573/1‑94 (MQ=60)

          ATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTC                                                                                 <  SRR4302547.1846930/94‑1 (MQ=60)

           TGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCC                                                                                >  SRR4302547.1389572/1‑94 (MQ=60)

           TGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCA                                                                                <  SRR4302547.1120421/94‑1 (MQ=60)

               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTGCATCATGCCCAAT                                                                            <  SRR4302547.3313724/94‑1 (MQ=60)

               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                            <  SRR4302547.3828461/94‑1 (MQ=60)

               GAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAAT                                                                            >  SRR4302547.4710335/1‑94 (MQ=60)

                AAAGTTTCCCGTCACGGCGTCCCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATC                                                                           <  SRR4302547.4973609/94‑1 (MQ=60)

                      TCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGC                                                                     <  SRR4302547.2655544/94‑1 (MQ=60)

                       CCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCG                                                                    <  SRR4302547.6246015/94‑1 (MQ=60)

                         CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                  >  SRR4302547.3098616/1‑94 (MQ=60)

                         CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                  <  SRR4302547.1087856/94‑1 (MQ=60)

                         CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                  >  SRR4302547.6687930/1‑94 (MQ=60)

                         CGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTG                                                                  <  SRR4302547.802000/94‑1 (MQ=60)

                           TCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTG                                                                <  SRR4302547.5623716/94‑1 (MQ=60)

                            CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                               >  SRR4302547.3312957/1‑94 (MQ=60)

                            CACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGT                                                               >  SRR4302547.219117/1‑94 (MQ=60)

                             ACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTT                                                              <  SRR4302547.602481/94‑1 (MQ=60)

                              CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                             <  SRR4302547.2450818/94‑1 (MQ=60)

                              CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                             >  SRR4302547.6003172/1‑94 (MQ=60)

                              CGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTT                                                             >  SRR4302547.3048011/1‑94 (MQ=60)

                               GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                            >  SRR4302547.534522/1‑94 (MQ=60)

                               GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                            <  SRR4302547.2569539/94‑1 (MQ=60)

                               GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTG                                                            >  SRR4302547.6002100/1‑94 (MQ=60)

                               GGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGGGTGTTTG                                                            >  SRR4302547.373390/1‑94 (MQ=60)

                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                         >  SRR4302547.1130651/1‑94 (MQ=60)

                                  GTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCT                                                         <  SRR4302547.2503876/94‑1 (MQ=60)

                                   TACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTA                                                        <  SRR4302547.934290/94‑1 (MQ=60)

                                     CCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACG                                                      >  SRR4302547.2577010/1‑94 (MQ=60)

                                      CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                     >  SRR4302547.5785079/1‑94 (MQ=60)

                                      CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                     <  SRR4302547.2627191/94‑1 (MQ=60)

                                      CGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGT                                                     >  SRR4302547.123108/1‑94 (MQ=60)

                                       GGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTC                                                    <  SRR4302547.4526981/94‑1 (MQ=60)

                                          TGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTAC                                                 <  SRR4302547.6772110/94‑1 (MQ=60)

                                           GCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACA                                                <  SRR4302547.594416/94‑1 (MQ=60)

                                            CGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAG                                               <  SRR4302547.4180702/94‑1 (MQ=60)

                                             GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                              >  SRR4302547.4792813/1‑94 (MQ=60)

                                             GGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGT                                              <  SRR4302547.3890531/94‑1 (MQ=60)

                                                 GTGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCA                                          >  SRR4302547.728640/1‑94 (MQ=60)

                                                  TGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                         <  SRR4302547.6378237/94‑1 (MQ=60)

                                                  TGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                         >  SRR4302547.2464017/1‑94 (MQ=60)

                                                  TGGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAG                                         >  SRR4302547.6029070/1‑94 (MQ=60)

                                                   GGCAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGC                                        <  SRR4302547.61257/94‑1 (MQ=60)

                                                     CAGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGT                                      >  SRR4302547.1397441/1‑94 (MQ=60)

                                                      AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                     >  SRR4302547.5043434/1‑94 (MQ=60)

                                                      AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                     <  SRR4302547.3610913/94‑1 (MQ=60)

                                                      AGTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTG                                     <  SRR4302547.3571446/94‑1 (MQ=60)

                                                       GTATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGC                                    <  SRR4302547.1075054/94‑1 (MQ=60)

                                                         ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                  <  SRR4302547.6762792/94‑1 (MQ=60)

                                                         ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                  >  SRR4302547.3212480/1‑94 (MQ=60)

                                                         ATCTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTT                                  <  SRR4302547.778460/94‑1 (MQ=60)

                                                           CTATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCC                                >  SRR4302547.3200101/1‑94 (MQ=60)

                                                            TATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCG                               >  SRR4302547.3345667/1‑94 (MQ=60)

                                                             ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                              <  SRR4302547.5699661/94‑1 (MQ=60)

                                                             ATTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGG                              <  SRR4302547.2270010/94‑1 (MQ=60)

                                                              TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                             >  SRR4302547.2304963/1‑94 (MQ=60)

                                                              TTGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGG                             >  SRR4302547.5426476/1‑94 (MQ=60)

                                                               TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                            >  SRR4302547.3475782/1‑94 (MQ=60)

                                                               TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                            <  SRR4302547.3312088/94‑1 (MQ=60)

                                                               TGCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGG                            >  SRR4302547.5918581/1‑94 (MQ=60)

                                                                GCTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGA                           >  SRR4302547.235479/1‑94 (MQ=60)

                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                          >  SRR4302547.982591/1‑94 (MQ=60)

                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                          >  SRR4302547.1180148/1‑94 (MQ=60)

                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                          <  SRR4302547.2245356/94‑1 (MQ=60)

                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                          <  SRR4302547.2877815/94‑1 (MQ=60)

                                                                 CTATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGAT                          >  SRR4302547.4103692/1‑94 (MQ=60)

                                                                  TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                         >  SRR4302547.482643/1‑94 (MQ=60)

                                                                  TATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATG                         <  SRR4302547.5651702/94‑1 (MQ=60)

                                                                   ATTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGG                        >  SRR4302547.2452449/1‑94 (MQ=60)

                                                                    TTCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGT                       >  SRR4302547.4970402/1‑94 (MQ=60)

                                                                     TCTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTG                      <  SRR4302547.4692822/94‑1 (MQ=60)

                                                                      CTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC                     <  SRR4302547.2666244/94‑1 (MQ=60)

                                                                      CTGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC                     >  SRR4302547.5772773/1‑94 (MQ=60)

                                                                       TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                    <  SRR4302547.1942642/94‑1 (MQ=60)

                                                                       TGCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCC                    >  SRR4302547.2847675/1‑94 (MQ=60)

                                                                        GCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                   >  SRR4302547.6694373/1‑94 (MQ=60)

                                                                        GCTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCA                   <  SRR4302547.5018351/94‑1 (MQ=60)

                                                                         ctgattgGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGAGATGGTGCCAT                  >  SRR4302547.6687307/8‑94 (MQ=60)

                                                                         CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                  >  SRR4302547.3028250/1‑94 (MQ=60)

                                                                         CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                  >  SRR4302547.4757082/1‑94 (MQ=60)

                                                                         CTAATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCAT                  >  SRR4302547.1123051/1‑94 (MQ=60)

                                                                           AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                >  SRR4302547.4417606/1‑94 (MQ=60)

                                                                           AATTGGCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGG                >  SRR4302547.1201988/1‑94 (MQ=60)

                                                                             TG‑GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTT             >  SRR4302547.4985434/1‑94 (MQ=60)

                                                                                GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGT           >  SRR4302547.813153/1‑94 (MQ=60)

                                                                                GCTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGT           <  SRR4302547.1634383/94‑1 (MQ=60)

                                                                                 CTCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTG          >  SRR4302547.3557364/1‑94 (MQ=60)

                                                                                  TCATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGA         <  SRR4302547.5135818/94‑1 (MQ=60)

                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT        >  SRR4302547.1614482/1‑94 (MQ=60)

                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT        >  SRR4302547.6374708/1‑94 (MQ=60)

                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT        >  SRR4302547.3758536/1‑94 (MQ=60)

                                                                                   CATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGAT        <  SRR4302547.5894983/94‑1 (MQ=60)

                                                                                    ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA       >  SRR4302547.6746160/1‑94 (MQ=60)

                                                                                    ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA       <  SRR4302547.601917/94‑1 (MQ=60)

                                                                                    ATGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATA       >  SRR4302547.3622837/1‑94 (MQ=60)

                                                                                     TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT      <  SRR4302547.5579098/94‑1 (MQ=60)

                                                                                     TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT      >  SRR4302547.1484998/1‑94 (MQ=60)

                                                                                     TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT      <  SRR4302547.3690084/94‑1 (MQ=60)

                                                                                     TGCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATAT      >  SRR4302547.2947693/1‑94 (MQ=60)

                                                                                      GCCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATT     >  SRR4302547.2416403/1‑94 (MQ=60)

                                                                                       CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG    <  SRR4302547.6496583/94‑1 (MQ=60)

                                                                                       CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG    <  SRR4302547.2540899/94‑1 (MQ=60)

                                                                                       CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG    <  SRR4302547.2549382/94‑1 (MQ=60)

                                                                                       CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG    <  SRR4302547.467797/94‑1 (MQ=60)

                                                                                       CCT‑GAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTG    >  SRR4302547.1099833/1‑94 (MQ=60)

                                                                                         ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA   <  SRR4302547.6502184/92‑1 (MQ=60)

                                                                                         ctGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGA   >  SRR4302547.1372265/3‑94 (MQ=60)

                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  <  SRR4302547.1804509/94‑1 (MQ=60)

                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  <  SRR4302547.6133661/94‑1 (MQ=60)

                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  >  SRR4302547.257519/1‑94 (MQ=60)

                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  >  SRR4302547.4565476/1‑94 (MQ=60)

                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  <  SRR4302547.2997723/94‑1 (MQ=60)

                                                                                          TGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  >  SRR4302547.3350904/1‑94 (MQ=60)

ACCGGCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATT‑GCTCATGCCTGGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGCCATGGTTTGTGATATTGAT  >  NC_000913/3979456‑3979638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`