Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,987,822	2 bp→CG	coding (578‑579/963 nt)	hemC ←	porphobilinogen deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,987,822	0	G	C	100.0%	989.5 / NA	275	G193A (GGC→GCG)	hemC	porphobilinogen deaminase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (131/144); total (131/144)
*	NC_000913	3,987,823	0	C	G	100.0%	985.7 / NA	275	G193A (GGC→GCG)	hemC	porphobilinogen deaminase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (130/145); total (130/145)

Only 100 of 281 total aligned reads displayed.

CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTTT  >  NC_000913/3987735‑3987908

||

cagcTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGgc                                                                                   <  1:690048/93‑1 (MQ=255)

cagcTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGgc                                                                                   <  2:2452026/93‑1 (MQ=255)

   cTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGcgcg                                                                                <  1:3636412/93‑1 (MQ=255)

   cTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGcgcg                                                                                >  1:4870856/1‑93 (MQ=255)

    tCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGa                                                                               >  2:1523094/1‑93 (MQ=255)

     cgcgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGaa                                                                              <  2:3151361/93‑1 (MQ=255)

     cgcgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGaa                                                                              <  1:5171038/93‑1 (MQ=255)

       cgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATa                                                                            <  1:2674314/93‑1 (MQ=255)

       cgAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATa                                                                            <  2:3280227/93‑1 (MQ=255)

        gAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATAc                                                                           >  2:2785434/1‑93 (MQ=255)

        gAGTGCGTGAATCATCAAGGCGGCATCCAATCCCCCCCGCACATTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATAc                                                                           <  1:3711681/93‑1 (MQ=255)

         aGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACg                                                                          >  1:3942318/1‑93 (MQ=255)

         aGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACg                                                                          >  2:4322322/1‑93 (MQ=255)

           tGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTg                                                                        >  2:3257543/1‑93 (MQ=255)

               tGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTc                                                                    >  1:5324131/1‑93 (MQ=255)

               tGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTc                                                                    >  1:2333900/1‑93 (MQ=255)

                gAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTcc                                                                   <  2:6035742/93‑1 (MQ=255)

                gAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTcc                                                                   <  1:6054795/93‑1 (MQ=255)

                gAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTcc                                                                   <  1:1917277/93‑1 (MQ=255)

                 aaTCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCa                                                                  <  2:3900753/93‑1 (MQ=255)

                   tcatcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGa                                                                >  2:4097006/1‑93 (MQ=255)

                   tcatcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGa                                                                >  1:1832599/1‑93 (MQ=255)

                   tcatcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGa                                                                >  2:2577536/1‑93 (MQ=255)

                     atcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGAcc                                                              >  2:4396927/1‑93 (MQ=255)

                      tcaAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCt                                                             >  2:6020356/1‑93 (MQ=255)

                       caAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTa                                                            >  2:1485676/1‑93 (MQ=255)

                        aaGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTaa                                                           <  1:77140/93‑1 (MQ=255)

                        aaGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTaa                                                           >  2:3848306/1‑93 (MQ=255)

                            cggcATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGt                                                       <  2:4875504/93‑1 (MQ=255)

                              gcATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGttt                                                     <  2:897696/93‑1 (MQ=255)

                               cATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGtttt                                                    <  2:660253/93‑1 (MQ=255)

                                 ttCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAg                                                  >  1:4230082/1‑93 (MQ=255)

                                 ttCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAg                                                  >  2:3959149/1‑93 (MQ=255)

                                  tCAATACCCCCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGt                                                 <  1:3769298/93‑1 (MQ=255)

                                    aaTCCCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTcc                                               <  2:2063151/93‑1 (MQ=255)

                                    aaTACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTcc                                               <  2:1449407/93‑1 (MQ=255)

                                     aTACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCg                                              <  2:3003110/93‑1 (MQ=255)

                                       aCCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGc                                            <  2:801034/93‑1 (MQ=255)

                                         ccACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTa                                          >  2:2175850/1‑93 (MQ=255)

                                         ccACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTa                                          <  1:5405321/93‑1 (MQ=255)

                                         ccACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTa                                          <  1:5965099/93‑1 (MQ=255)

                                         ccACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTa                                          <  2:4070028/93‑1 (MQ=255)

                                          cACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAc                                         <  2:2022744/93‑1 (MQ=255)

                                           aCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTCCg                                        >  2:3813624/1‑93 (MQ=255)

                                           aCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACg                                        >  1:129662/1‑93 (MQ=255)

                                           aCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACg                                        >  1:5696148/1‑93 (MQ=255)

                                             cGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGc                                      >  1:1198763/1‑93 (MQ=255)

                                             cGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGc                                      >  1:3519765/1‑93 (MQ=255)

                                              gCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCa                                     <  2:2770132/93‑1 (MQ=255)

                                               cACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCaa                                    <  1:2710973/93‑1 (MQ=255)

                                               cACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCaa                                    <  2:456326/93‑1 (MQ=255)

                                                aCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAg                                   >  2:1220138/1‑93 (MQ=255)

                                                aCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAg                                   >  2:3656354/1‑93 (MQ=255)

                                                aCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAg                                   >  1:492947/1‑93 (MQ=255)

                                                aCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAg                                   >  2:1934447/1‑93 (MQ=255)

                                                aCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAg                                   >  1:1810927/1‑93 (MQ=255)

                                                 ccTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGa                                  >  2:5130602/1‑93 (MQ=255)

                                                 ccTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGa                                  >  1:4661521/1‑93 (MQ=255)

                                                 ccTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGa                                  >  2:2169491/1‑93 (MQ=255)

                                                  cTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGaa                                 >  2:5219475/1‑93 (MQ=255)

                                                  cTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGaa                                 <  2:3059758/93‑1 (MQ=255)

                                                   ttGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAat                                <  1:2176208/93‑1 (MQ=255)

                                                   ttGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAat                                >  2:6084827/1‑93 (MQ=255)

                                                    tGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatg                               <  2:2501216/93‑1 (MQ=255)

                                                     gTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatga                              >  1:5062415/1‑93 (MQ=255)

                                                      tCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatgat                             <  1:3569468/93‑1 (MQ=255)

                                                       ccTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatgatg                            <  2:538638/93‑1 (MQ=255)

                                                       ccTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAatgatg                            >  1:2869521/1‑93 (MQ=255)

                                                        cTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATgg                           >  1:3036933/1‑93 (MQ=255)

                                                        cTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATgg                           <  2:2280197/93‑1 (MQ=255)

                                                         tACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGc                          >  1:4136774/1‑93 (MQ=255)

                                                         tACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGc                          >  2:1196676/1‑93 (MQ=255)

                                                         caCCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGc                          <  2:1587752/92‑1 (MQ=255)

                                                          aCCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCa                         <  1:743420/93‑1 (MQ=255)

                                                          aCCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCa                         <  1:883675/93‑1 (MQ=255)

                                                          aCCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCa                         >  1:634639/1‑93 (MQ=255)

                                                           ccgccgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAt                        <  1:3394690/93‑1 (MQ=255)

                                                           ccgccgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAt                        >  1:2396311/1‑93 (MQ=255)

                                                           ccgccgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAt                        <  1:4622125/93‑1 (MQ=255)

                                                           ccgccgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGGTTTAGTCCGGCTACGGCAAGTATGATGTCAt                        >  1:2571523/1‑93 (MQ=255)

                                                             gccgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCg                      >  2:2695688/1‑93 (MQ=255)

                                                               cgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTa                    >  1:4259673/1‑93 (MQ=255)

                                                               cgGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTa                    >  2:2959155/1‑93 (MQ=255)

                                                                  aaGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTc                 <  1:177613/93‑1 (MQ=255)

                                                                  aaGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTc                 >  2:5675776/1‑93 (MQ=255)

                                                                     agaAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGcc              <  1:1444079/93‑1 (MQ=255)

                                                                      gaAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCg             >  2:4938539/1‑93 (MQ=255)

                                                                       aaaTCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGt            <  2:3706746/93‑1 (MQ=255)

                                                                         aTCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTa          >  1:5268290/1‑93 (MQ=255)

                                                                          tctcGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTAt         >  2:4642382/1‑93 (MQ=255)

                                                                           ctcGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATc        >  2:135826/1‑93 (MQ=255)

                                                                            tcGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATcc       <  1:3120496/93‑1 (MQ=255)

                                                                            tcGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATcc       >  1:5518427/1‑93 (MQ=255)

                                                                              gggTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCTCCGTTATCCAg     <  1:1953983/93‑1 (MQ=255)

                                                                              gggTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAg     <  1:4510513/93‑1 (MQ=255)

                                                                              gggTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAg     >  1:2811361/1‑93 (MQ=255)

                                                                               ggTGGCACCGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGt    <  1:1560202/93‑1 (MQ=255)

                                                                               ggTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGt    <  1:4098294/93‑1 (MQ=255)

                                                                                gTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCTCCGTTATCCAGtt   <  2:3372132/93‑1 (MQ=255)

                                                                                gTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGtt   <  2:2948497/93‑1 (MQ=255)

||

CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTTT  >  NC_000913/3987735‑3987908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTTT  >  NC_000913/3987729‑3987908

GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAC                                                                                        >  SRR4302532.565631/1‑94 (MQ=60)

GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAC                                                                                        >  SRR4302532.565630/1‑94 (MQ=60)

GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAC                                                                                        >  SRR4302532.2964530/1‑94 (MQ=60)

GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAC                                                                                        <  SRR4302532.5385258/94‑1 (MQ=60)

      CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGTGTGGCAACGCGGCG                                                                                  <  SRR4302532.2893535/94‑1 (MQ=60)

      CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCG                                                                                  <  SRR4302532.713730/94‑1 (MQ=60)

      CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCG                                                                                  <  SRR4302532.58219/94‑1 (MQ=60)

      CAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCG                                                                                  <  SRR4302532.4871601/94‑1 (MQ=60)

      CACCTCGCGATTACGTGAATCATCAATGCGGCATTCAATACCCACCGCCCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCG                                                                                  <  SRR4302532.58220/94‑1 (MQ=60)

       AGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGC                                                                                 >  SRR4302532.1330933/1‑94 (MQ=60)

       AGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGC                                                                                 >  SRR4302532.2796219/1‑94 (MQ=60)

        GCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCG                                                                                >  SRR4302532.5038316/1‑94 (MQ=60)

        GCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCG                                                                                <  SRR4302532.1690658/94‑1 (MQ=60)

         CTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGA                                                                               <  SRR4302532.3758412/94‑1 (MQ=60)

           CGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAAT                                                                             <  SRR4302532.5349138/94‑1 (MQ=60)

           CGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAAT                                                                             <  SRR4302532.4916956/94‑1 (MQ=60)

            GCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATA                                                                            >  SRR4302532.5596915/1‑94 (MQ=60)

             CGAGTGCGTGAATCATCAAGGCGGCATTCAATCCCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATAC                                                                           <  SRR4302532.5582179/94‑1 (MQ=60)

             CGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATAC                                                                           >  SRR4302532.2956392/1‑94 (MQ=60)

             CGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATAC                                                                           <  SRR4302532.2764075/94‑1 (MQ=60)

              GAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACG                                                                          <  SRR4302532.6314803/94‑1 (MQ=60)

              GAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACG                                                                          >  SRR4302532.4075165/1‑94 (MQ=60)

              GAGTGCGTGAATCATCAAGGCGGCATCCAATCCCCCCCGCACATTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACG                                                                          <  SRR4302532.3836267/94‑1 (MQ=60)

               AGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGT                                                                         >  SRR4302532.5984361/1‑94 (MQ=60)

                GTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTG                                                                        <  SRR4302532.1449858/94‑1 (MQ=60)

                 TGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGA                                                                       >  SRR4302532.2836524/1‑94 (MQ=60)

                  GCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGAC                                                                      >  SRR4302532.3978921/1‑94 (MQ=60)

                   CGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACT                                                                     >  SRR4302532.3832444/1‑94 (MQ=60)

                    GTGAATCATCAAGGCGGCATTCAATCCCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                                    <  SRR4302532.3162666/94‑1 (MQ=60)

                    GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                                    >  SRR4302532.2411637/1‑94 (MQ=60)

                    GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                                    >  SRR4302532.5392380/1‑94 (MQ=60)

                    GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                                    >  SRR4302532.5508623/1‑94 (MQ=60)

                    GTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTC                                                                    >  SRR4302532.2875472/1‑94 (MQ=60)

                      GAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCA                                                                  <  SRR4302532.1981030/94‑1 (MQ=60)

                      GAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCA                                                                  <  SRR4302532.1981029/94‑1 (MQ=60)

                      GAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCA                                                                  <  SRR4302532.6263868/94‑1 (MQ=60)

                      GAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCA                                                                  <  SRR4302532.3736364/94‑1 (MQ=60)

                        ATCATCAAGGCTGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGA                                                                <  SRR4302532.4456978/94‑1 (MQ=60)

                        ATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGA                                                                >  SRR4302532.1893551/1‑94 (MQ=60)

                        ATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGA                                                                >  SRR4302532.4375503/1‑94 (MQ=60)

                           ATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCT                                                             >  SRR4302532.4689478/1‑94 (MQ=60)

                              AAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAA                                                          <  SRR4302532.79891/94‑1 (MQ=60)

                               AGGCGACATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGGCCTAAAC                                                         <  SRR4302532.1982163/94‑1 (MQ=60)

                                    GCATTCAATACCCACCGCACCTTGTCCTACCGCCTGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTT                                                    <  SRR4302532.5325983/94‑1 (MQ=60)

                                    GCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTT                                                    <  SRR4302532.3856158/94‑1 (MQ=60)

                                    GCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTT                                                    <  SRR4302532.3148013/94‑1 (MQ=60)

                                    GCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGAACTAAACGTTTT                                                    <  SRR4302532.6071530/94‑1 (MQ=60)

                                     CATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTA                                                   >  SRR4302532.6207074/1‑94 (MQ=60)

                                      ATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAG                                                  >  SRR4302532.1343914/1‑94 (MQ=60)

                                      ATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAG                                                  >  SRR4302532.4372080/1‑94 (MQ=60)

                                       TTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGT                                                 >  SRR4302532.4710483/1‑94 (MQ=60)

                                       TTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGT                                                 <  SRR4302532.2988286/94‑1 (MQ=60)

                                        TCAATACCCCCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTC                                                <  SRR4302532.3896157/94‑1 (MQ=60)

                                        TCAATACCCACCGCACCTGGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTC                                                <  SRR4302532.4384949/94‑1 (MQ=60)

                                         cactcCCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                               <  SRR4302532.1849138/89‑1 (MQ=60)

                                         CAATACCCCCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                               <  SRR4302532.2773111/94‑1 (MQ=60)

                                         CAATACCCACCGCTCCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCCACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                               <  SRR4302532.789508/94‑1 (MQ=60)

                                         CAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                               >  SRR4302532.719948/1‑94 (MQ=60)

                                         CAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                               >  SRR4302532.4788991/1‑94 (MQ=60)

                                         CAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCC                                               >  SRR4302532.3808267/1‑94 (MQ=60)

                                          AATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCG                                              >  SRR4302532.4096556/1‑94 (MQ=60)

                                          AATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCG                                              <  SRR4302532.3679474/94‑1 (MQ=60)

                                          AATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCG                                              >  SRR4302532.2757648/1‑94 (MQ=60)

                                          AATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCG                                              <  SRR4302532.444277/94‑1 (MQ=60)

                                           ATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGG                                             >  SRR4302532.791820/1‑94 (MQ=60)

                                           ATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGG                                             >  SRR4302532.513880/1‑94 (MQ=60)

                                           ATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGG                                             >  SRR4302532.3410639/1‑94 (MQ=60)

                                             ACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCT                                           <  SRR4302532.6306880/94‑1 (MQ=60)

                                               CCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAC                                         <  SRR4302532.5592593/94‑1 (MQ=60)

                                               CCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTAC                                         <  SRR4302532.6171362/94‑1 (MQ=60)

                                                CACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                                        >  SRR4302532.134049/1‑94 (MQ=60)

                                                CACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                                        >  SRR4302532.2116017/1‑94 (MQ=60)

                                                CACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                                        <  SRR4302532.963521/94‑1 (MQ=60)

                                                CACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                                        >  SRR4302532.5894857/1‑94 (MQ=60)

                                                CACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                                        <  SRR4302532.4324937/94‑1 (MQ=60)

                                                CACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACG                                        <  SRR4302532.2479020/94‑1 (MQ=60)

                                                 CCCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGG                                       <  SRR4302532.3352192/94‑1 (MQ=60)

                                                 ACCGGACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTTGCACTGCGGCGCGAATTCGTGACGCCAGACCTAAACGTGTTAGTCCGGCTACGG                                       >  SRR4302532.4584977/1‑94 (MQ=60)

                                                 ACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGG                                       >  SRR4302532.2993311/1‑94 (MQ=60)

                                                  CCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGC                                      >  SRR4302532.3637603/1‑94 (MQ=60)

                                                  CCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGC                                      >  SRR4302532.1238852/1‑94 (MQ=60)

                                                    GCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAA                                    >  SRR4302532.5773805/1‑94 (MQ=60)

                                                    GCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAA                                    <  SRR4302532.5086607/94‑1 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.5279219/1‑94 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.510173/1‑94 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.510172/1‑94 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.2640550/1‑94 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.1871320/1‑94 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.5458968/1‑94 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   <  SRR4302532.2802146/94‑1 (MQ=60)

                                                     CACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAG                                   >  SRR4302532.1871321/1‑94 (MQ=60)

                                                      ACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGA                                  >  SRR4302532.4820453/1‑94 (MQ=60)

                                                        CTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAAT                                >  SRR4302532.860288/1‑94 (MQ=60)

                                                         TTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATG                               <  SRR4302532.2248895/94‑1 (MQ=60)

                                                          TGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGA                              >  SRR4302532.5236218/1‑94 (MQ=60)

                                                           GTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGAT                             <  SRR4302532.2420214/94‑1 (MQ=60)

                                                           GTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGAT                             <  SRR4302532.436549/94‑1 (MQ=60)

                                                            TCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATG                            <  SRR4302532.3688915/94‑1 (MQ=60)

                                                            TCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATG                            >  SRR4302532.2965487/1‑94 (MQ=60)

                                                            TCCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATG                            <  SRR4302532.675274/94‑1 (MQ=60)

                                                             CCTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGG                           >  SRR4302532.3137141/1‑94 (MQ=60)

                                                              CTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGC                          >  SRR4302532.4275540/1‑94 (MQ=60)

                                                              CTACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGC                          <  SRR4302532.3967958/94‑1 (MQ=60)

                                                               TACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCA                         >  SRR4302532.656784/1‑94 (MQ=60)

                                                               TACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTACGGCTACGGCAAGAATGATGGCA                         >  SRR4302532.3511858/1‑94 (MQ=60)

                                                               CACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCA                         <  SRR4302532.1559600/94‑1 (MQ=60)

                                                                ACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAT                        <  SRR4302532.768841/94‑1 (MQ=60)

                                                                ACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAT                        <  SRR4302532.913885/94‑1 (MQ=60)

                                                                ACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCAT                        >  SRR4302532.2476111/1‑94 (MQ=60)

                                                                ACCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGGTTTAGTCCGGCTACGGCAAGTATGATGTCAT                        >  SRR4302532.2657394/1‑94 (MQ=60)

                                                                 CCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC                       <  SRR4302532.3508267/94‑1 (MQ=60)

                                                                 CCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC                       <  SRR4302532.2321773/94‑1 (MQ=60)

                                                                 CCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC                       <  SRR4302532.4779736/94‑1 (MQ=60)

                                                                 CCGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATC                       <  SRR4302532.1910998/94‑1 (MQ=60)

                                                                  CGCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCG                      >  SRR4302532.4262542/1‑94 (MQ=60)

                                                                   GCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGT                     <  SRR4302532.2679666/94‑1 (MQ=60)

                                                                   GCCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGT                     >  SRR4302532.1889394/1‑94 (MQ=60)

                                                                    CCGGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTA                    >  SRR4302532.4402977/1‑94 (MQ=60)

                                                                      GGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATT                  >  SRR4302532.2217332/1‑94 (MQ=60)

                                                                      GGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATT                  <  SRR4302532.367191/94‑1 (MQ=60)

                                                                      GGAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATT                  >  SRR4302532.5455566/1‑94 (MQ=60)

                                                                       GAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTC                 <  SRR4302532.5662085/94‑1 (MQ=60)

                                                                       GAAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTC                 <  SRR4302532.1727757/94‑1 (MQ=60)

                                                                        AAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCG                <  SRR4302532.2254101/94‑1 (MQ=60)

                                                                        AAGAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCG                <  SRR4302532.183566/94‑1 (MQ=60)

                                                                          GAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCC              <  SRR4302532.674711/94‑1 (MQ=60)

                                                                          GAGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCC              <  SRR4302532.1531429/94‑1 (MQ=60)

                                                                           AGAAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCG             <  SRR4302532.1492785/94‑1 (MQ=60)

                                                                             AAATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTT           >  SRR4302532.518009/1‑94 (MQ=60)

                                                                              AATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTA          >  SRR4302532.5449959/1‑94 (MQ=60)

                                                                               ATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTAT         <  SRR4302532.3626820/94‑1 (MQ=60)

                                                                               ATCTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTAT         <  SRR4302532.5554469/94‑1 (MQ=60)

                                                                                 CTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCC       <  SRR4302532.752015/94‑1 (MQ=60)

                                                                                 CTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCC       >  SRR4302532.979671/1‑94 (MQ=60)

                                                                                 CTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCC       >  SRR4302532.5709929/1‑94 (MQ=60)

                                                                                 CTCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCC       >  SRR4302532.1846115/1‑94 (MQ=60)

                                                                                  TCGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCA      <  SRR4302532.3224034/94‑1 (MQ=60)

                                                                                   CGGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAG     >  SRR4302532.2905488/1‑94 (MQ=60)

                                                                                    GGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCTCCGTTATCCAGT    <  SRR4302532.2018898/94‑1 (MQ=60)

                                                                                    GGGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGT    <  SRR4302532.4664513/94‑1 (MQ=60)

                                                                                    GGGTGGCAACGCGCCGCGAATACGTGACTCCCGACCTAAACGATTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCCTTATCCAGT    >  SRR4302532.1931638/1‑94 (MQ=60)

                                                                                     GGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTT   <  SRR4302532.3837099/94‑1 (MQ=60)

                                                                                     GGTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTT   <  SRR4302532.4235871/94‑1 (MQ=60)

                                                                                      GTGGCAACGCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTTT  <  SRR4302532.4113792/94‑1 (MQ=60)

GGCAAGCAGCTCGCGAGTGCGTGAATCATCAAGGCGGCATTCAATACCCACCGCACCTTGTCCTACCGCCGGAAGAGAAATCTCGGGTGGCAAGCCGGCGCGAATACGTGACTCCAGACCTAAACGTTTTAGTCCGGCTACGGCAAGAATGATGGCATCGTATTCGCCGTTATCCAGTTT  >  NC_000913/3987729‑3987908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`