Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 521 238.2 12299412 96.0% 11807435 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,001,717 +C coding (86/903 nt) rarD ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,001,7171.C100.0% 601.0 / NA 174T29R (ACG→AGG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (75/99);  total (75/99)

Only 100 of 200 total aligned reads displayed.

TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT  >  NC_000913/4001625‑4001803
                                                                                              |                                                                                      
tGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑g                                                                                         >  1:2635748/1‑93 (MQ=255)
tGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑g                                                                                         >  1:2120530/1‑93 (MQ=255)
 gCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑gc                                                                                        <  2:495630/93‑2 (MQ=255)
  cgcatcaCGACCACCATAAAGAAAAACGGCCGGATCACGCGAGGCGCCAGGATTTCATCGGCGGGCAGGTAGTAAATCAACTTGAAGTAC‑gct                                                                                       <  1:5302015/91‑3 (MQ=255)
  cTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑gct                                                                                       <  2:5804343/93‑3 (MQ=255)
   tcatcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑gctg                                                                                      <  2:4050605/93‑4 (MQ=255)
       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGgcgc                                                                                  <  2:6087076/93‑1 (MQ=255)
       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGgcgc                                                                                  <  1:5034771/93‑1 (MQ=255)
       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGgcgc                                                                                  <  2:320132/93‑1 (MQ=255)
       caGCACCACCATAAAAAAAAACCACCATATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGgcgc                                                                                  <  2:1357377/93‑1 (MQ=255)
         gCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATAAACTTGAAGTAC‑GCTGGCGCta                                                                                >  2:1550300/1‑93 (MQ=255)
          caccacCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCtat                                                                               <  2:271033/93‑1 (MQ=255)
          caccacCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGGAGTAAATCAACTTGAAGTAC‑GCTGGCGCtat                                                                               >  2:4902461/1‑93 (MQ=255)
           accaccATAAAGAGAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCtata                                                                              <  2:533372/93‑1 (MQ=255)
           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCtata                                                                              >  1:1659515/1‑93 (MQ=255)
           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCtata                                                                              <  1:3890732/93‑1 (MQ=255)
             caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAcc                                                                            >  1:2053391/1‑93 (MQ=255)
             caccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAcc                                                                            >  1:218967/1‑93 (MQ=255)
               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAcccc                                                                          >  2:2899598/1‑93 (MQ=255)
               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATAGGCGGGCCCGTAGTAAAAAAACTTAAAGTACTC‑TGGCGCTATcccca                                                                          >  1:3186417/1‑92 (MQ=255)
                caTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCa                                                                         <  1:1348725/93‑1 (MQ=255)
                  tAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaa                                                                       >  1:4759395/1‑93 (MQ=255)
                  aaaaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaa                                                                       <  2:2510150/92‑1 (MQ=255)
                   aaaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCCATACCCCcaat                                                                      >  1:2526726/1‑93 (MQ=255)
                     agagAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaataa                                                                    <  1:5459556/93‑1 (MQ=255)
                     aGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaataa                                                                    >  2:823429/1‑93 (MQ=255)
                      gAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaataaa                                                                   >  1:5177439/1‑93 (MQ=255)
                       aaaaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaataaaa                                                                  <  2:3824356/93‑1 (MQ=255)
                        aaaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaataaaat                                                                 >  1:6038209/1‑93 (MQ=255)
                        aaaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCaaataaaat                                                                 <  2:1125211/93‑1 (MQ=255)
                           accaCCAGAGCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAg                                                              <  2:528784/90‑1 (MQ=255)
                           aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAg                                                              <  2:2365572/93‑1 (MQ=255)
                           aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAg                                                              <  1:1519339/93‑1 (MQ=255)
                           aCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAg                                                              <  2:1950424/93‑1 (MQ=255)
                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCg                                                            <  1:74478/93‑1 (MQ=255)
                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCg                                                            <  1:1359258/93‑1 (MQ=255)
                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCg                                                            >  1:805341/1‑93 (MQ=255)
                              aCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCgg                                                           >  1:3789484/1‑93 (MQ=255)
                                cAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCa                                                         <  1:2224597/93‑1 (MQ=255)
                                cAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCa                                                         >  1:1647120/1‑93 (MQ=255)
                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                        <  2:4300379/93‑1 (MQ=255)
                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                        <  2:2148424/93‑1 (MQ=255)
                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGCAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGGCGCaa                                                        >  1:3370391/1‑93 (MQ=255)
                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  1:564322/93‑1 (MQ=255)
                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  1:63172/93‑1 (MQ=255)
                                     cACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGc                                                    <  2:2096557/93‑1 (MQ=255)
                                      aCGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAACCCAAATAAAATAAGCGGCCAGAGCg                                                   >  1:4397278/1‑93 (MQ=255)
                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCCAATAAAATAAACGGCAAgagaga                                                  >  2:2439723/1‑93 (MQ=255)
                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGa                                                  >  2:4829726/1‑93 (MQ=255)
                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGa                                                  <  2:5616769/93‑1 (MQ=255)
                                       cgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGa                                                  >  2:2947670/1‑93 (MQ=255)
                                        gcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGGGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAg                                                 >  1:4213361/1‑93 (MQ=255)
                                        gcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAg                                                 <  1:3277419/93‑1 (MQ=255)
                                          gATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTa                                               <  1:4141697/93‑1 (MQ=255)
                                           aTGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTaa                                              >  2:2763222/1‑93 (MQ=255)
                                           aTGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTaa                                              <  2:2303260/93‑1 (MQ=255)
                                             gCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGGAATa                                            >  2:3136813/1‑93 (MQ=255)
                                             gCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATa                                            <  1:2146284/93‑1 (MQ=255)
                                              cGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAc                                           <  1:3918166/93‑1 (MQ=255)
                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGGGAGTAATACGc                                         >  1:1858405/1‑93 (MQ=255)
                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGc                                         <  2:2335131/93‑1 (MQ=255)
                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGc                                         >  1:1468559/1‑93 (MQ=255)
                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGc                                         <  2:5236186/93‑1 (MQ=255)
                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                       <  1:727250/93‑1 (MQ=255)
                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCCAATAAAATAAGCGGCAAGAGCGAGGAATACGCCCt                                      >  1:1257730/1‑93 (MQ=255)
                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                      <  2:2643576/93‑1 (MQ=255)
                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                      <  2:638509/93‑1 (MQ=255)
                                                    gATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTAC‑GCTGGCGCTCTACCCCAAATAAAATAATTGGCCAGAGCGAGTAATACGCCCtc                                     >  1:2807794/1‑92 (MQ=255)
                                                     aTTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGGGAGTAATACGCCCTGc                                    >  1:6042139/1‑93 (MQ=255)
                                                       ttCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcg                                  >  2:2047488/1‑93 (MQ=255)
                                                       ttCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAA‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAAAACGAGTAATACGCCCTGCcg                                  >  2:4403912/1‑93 (MQ=255)
                                                         cATCGGCGGGCACGTAGTAAATCAACTTGAAGTAA‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcgcg                                >  2:5629025/1‑93 (MQ=255)
                                                            cggcggGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgttt                             <  1:2756605/93‑1 (MQ=255)
                                                            cggcggGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGAGAGTAATACGCCCTGCCGCgttt                             >  1:3492048/1‑93 (MQ=255)
                                                              gcggGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt                           >  2:3282322/1‑93 (MQ=255)
                                                              gcggGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt                           <  2:1470186/93‑1 (MQ=255)
                                                                gggCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGCGCGCGTAATACGCCCTGCCGCgttcgttt                         >  2:5923001/1‑93 (MQ=255)
                                                                  gCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTg                       <  1:4596228/93‑1 (MQ=255)
                                                                   cACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGc                      >  2:1601145/1‑93 (MQ=255)
                                                                   cACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGc                      <  1:2271274/93‑1 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc                   >  2:3618497/1‑93 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc                   <  2:601815/93‑1 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc                   <  2:3606703/93‑1 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGGTTTGcatc                   >  1:5390618/1‑93 (MQ=255)
                                                                        agtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                 <  2:4501604/93‑1 (MQ=255)
                                                                        agtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                 >  1:3565485/1‑93 (MQ=255)
                                                                        agtaAATCAACTTGAAGTAA‑GCTGGCGCTATACGCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                 <  1:5810643/93‑1 (MQ=255)
                                                                         gtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                <  2:4909774/93‑1 (MQ=255)
                                                                         gtaAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                <  2:1788225/93‑1 (MQ=255)
                                                                          taAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc               >  2:5695262/1‑93 (MQ=255)
                                                                           aaaTCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCg              <  1:1440671/93‑1 (MQ=255)
                                                                               cAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGaa          <  2:765067/93‑1 (MQ=255)
                                                                                aaCTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAt         >  2:5528820/1‑93 (MQ=255)
                                                                                 aCTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATa        <  1:244840/93‑1 (MQ=255)
                                                                                    tGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTc     >  2:539530/1‑93 (MQ=255)
                                                                                     gAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATAGGGAATACTCa    >  2:4092685/1‑93 (MQ=255)
                                                                                      aaGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt   >  2:1112555/1‑93 (MQ=255)
                                                                                      aaGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt   >  1:1526586/1‑93 (MQ=255)
                                                                                      aaGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt   <  1:1735010/93‑1 (MQ=255)
                                                                                       aGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAtt  <  2:3050582/93‑1 (MQ=255)
                                                                                              |                                                                                      
TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT  >  NC_000913/4001625‑4001803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT  >  NC_000913/4001624‑4001806
                                                                                             |                                                                                          
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG                                                                                            >  SRR4302532.2191373/1‑94 (MQ=60)
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG                                                                                            >  SRR4302532.2724071/1‑94 (MQ=60)
   cgcatcacgACCACCATAAAGAAAAACGGCCGGATCACGCGAGGCGCCAGGATTTCATCGGCGGGCAGGTAGTAAATCAACTTGAAGTACGctg                                                                                         <  SRR4302532.5485249/85‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGctg                                                                                         >  SRR4302532.3980436/1‑91 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCT                                                                                    >  SRR4302532.5903028/1‑94 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCT                                                                                    <  SRR4302532.5207452/94‑1 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCT                                                                                    >  SRR4302532.3252738/1‑94 (MQ=60)
           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATA                                                                                 >  SRR4302532.1715760/1‑94 (MQ=60)
            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAC                                                                                <  SRR4302532.4021999/94‑1 (MQ=60)
             CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACC                                                                               >  SRR4302532.2121775/1‑94 (MQ=60)
             CCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACC                                                                               >  SRR4302532.226437/1‑94 (MQ=60)
               ACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCC                                                                             >  SRR4302532.5593412/1‑94 (MQ=60)
                 CATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAA                                                                           <  SRR4302532.1393822/94‑1 (MQ=60)
                  ATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAA                                                                          >  SRR4302532.4922271/1‑94 (MQ=60)
                  ATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAA                                                                          >  SRR4302532.2297544/1‑94 (MQ=60)
                   TAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTCAATCAACTTGAAGTACGCTGGCGCCATACCCCCAAT                                                                         >  SRR4302532.1522569/1‑94 (MQ=60)
                   TAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCCATACCCCCAAT                                                                         >  SRR4302532.2611028/1‑94 (MQ=60)
                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATA                                                                        <  SRR4302532.5085874/94‑1 (MQ=60)
                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATA                                                                        >  SRR4302532.2259573/1‑94 (MQ=60)
                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATA                                                                        <  SRR4302532.90688/94‑1 (MQ=60)
                      AGAGAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAA                                                                      <  SRR4302532.5648839/94‑1 (MQ=60)
                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAA                                                                      >  SRR4302532.5355735/1‑94 (MQ=60)
                       AAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAA                                                                     <  SRR4302532.1508299/94‑1 (MQ=60)
                        AAAAACGAGCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAAT                                                                    <  SRR4302532.1358782/94‑1 (MQ=60)
                        AAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAAT                                                                    >  SRR4302532.6246751/1‑94 (MQ=60)
                         AAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATA                                                                   <  SRR4302532.4551612/94‑1 (MQ=60)
                          AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAA                                                                  <  SRR4302532.5745409/94‑1 (MQ=60)
                          AAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAA                                                                  <  SRR4302532.3863631/94‑1 (MQ=60)
                            ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGC                                                                <  SRR4302532.1570639/94‑1 (MQ=60)
                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCG                                                               >  SRR4302532.832827/1‑94 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGG                                                              <  SRR4302532.1404773/94‑1 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGG                                                              >  SRR4302532.3917332/1‑94 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGG                                                              <  SRR4302532.77142/94‑1 (MQ=60)
                               ACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCGATACCCCAAATAAAATAAGCGGC                                                             >  SRR4302532.3618430/1‑94 (MQ=60)
                                CCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCA                                                            >  SRR4302532.829147/1‑94 (MQ=60)
                                CCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCA                                                            >  SRR4302532.1702926/1‑94 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAA                                                           <  SRR4302532.2298428/94‑1 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGCAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGGCGCAA                                                           >  SRR4302532.3483076/1‑94 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACCTAGTAAATCAAATTGAAGCACGCTCGCGCTTTACCCCAAATAAAATAAGCcgcca                                                           >  SRR4302532.4777366/1‑89 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                        <  SRR4302532.65445/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                        <  SRR4302532.584006/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                        <  SRR4302532.1571543/94‑1 (MQ=60)
                                      CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAACCCAAATAAAATAAGCGGCCAGAGCG                                                      >  SRR4302532.4546910/1‑94 (MQ=60)
                                        CGCGATGCTGCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                    <  SRR4302532.334430/94‑1 (MQ=60)
                                        CGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGGGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAG                                                    >  SRR4302532.4354781/1‑94 (MQ=60)
                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGT                                                   <  SRR4302532.3386423/94‑1 (MQ=60)
                                          CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTA                                                  <  SRR4302532.1396764/94‑1 (MQ=60)
                                           GATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAA                                                 <  SRR4302532.4280639/94‑1 (MQ=60)
                                           GATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAAAAAGCGGCAAGAGCGAGTAA                                                 >  SRR4302532.4397766/1‑94 (MQ=60)
                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                              <  SRR4302532.1711396/94‑1 (MQ=60)
                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                              <  SRR4302532.283699/94‑1 (MQ=60)
                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                              <  SRR4302532.2217999/94‑1 (MQ=60)
                                               CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACG                                             <  SRR4302532.4050194/94‑1 (MQ=60)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGGGAGTAATACGC                                            >  SRR4302532.1920020/1‑94 (MQ=60)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                            >  SRR4302532.1518032/1‑94 (MQ=60)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                            >  SRR4302532.3650698/1‑94 (MQ=60)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                            >  SRR4302532.2884355/1‑94 (MQ=60)
                                                CTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                            >  SRR4302532.2374735/1‑94 (MQ=60)
                                                  CAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCC                                          >  SRR4302532.6248999/1‑94 (MQ=60)
                                                  CAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAACGGCAAGAGCGAGTAATAAGCCC                                          >  SRR4302532.158299/1‑94 (MQ=60)
                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCCAATAAAATAAGCGGCAAGAGCGAGGAATACGCCCT                                         >  SRR4302532.1299809/1‑94 (MQ=60)
                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                         <  SRR4302532.2208617/94‑1 (MQ=60)
                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                         <  SRR4302532.752037/94‑1 (MQ=60)
                                                    GGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGGAATACGCCCTC                                        >  SRR4302532.4346769/1‑94 (MQ=60)
                                                    GGATTTCATCGGCGGGCACGTAGGAAATCAACTTGAAGTACGCTGGCGCTCTACCCCAAATAAAATAATTGGCCAGAGCGAGTAATACGCCCTC                                        >  SRR4302532.2901826/1‑94 (MQ=60)
                                                     GATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGGGAGTAATACGCCCTGC                                       >  SRR4302532.6250771/1‑94 (MQ=60)
                                                      ATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCC                                      >  SRR4302532.1746722/1‑94 (MQ=60)
                                                           ATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                 >  SRR4302532.4076863/1‑94 (MQ=60)
                                                           ATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                 <  SRR4302532.1454234/94‑1 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGAGAGTAATACGCCCTGCCGCGTTT                                >  SRR4302532.3609055/1‑94 (MQ=60)
                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                               <  SRR4302532.6033577/94‑1 (MQ=60)
                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                               <  SRR4302532.2849174/94‑1 (MQ=60)
                                                               GCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                             <  SRR4302532.5546822/94‑1 (MQ=60)
                                                                   GCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGC                         <  SRR4302532.4752915/94‑1 (MQ=60)
                                                                    CACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                        <  SRR4302532.2346646/94‑1 (MQ=60)
                                                                    CACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                        <  SRR4302532.2346645/94‑1 (MQ=60)
                                                                      CGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                      >  SRR4302532.2635562/1‑94 (MQ=60)
                                                                      CGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGGTTTGCATC                      >  SRR4302532.5577368/1‑94 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                    >  SRR4302532.3684780/1‑94 (MQ=60)
                                                                        TAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCA                    <  SRR4302532.6275580/94‑1 (MQ=60)
                                                                         AGTAAATCAACTTGAAGTAAGCTGGCGCTATACGCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                   <  SRR4302532.6012507/94‑1 (MQ=60)
                                                                            AAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                >  SRR4302532.5224841/1‑94 (MQ=60)
                                                                            AAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                <  SRR4302532.1489227/94‑1 (MQ=60)
                                                                              ATCAACTTGAAGTACGCTGGAGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGA              >  SRR4302532.2590261/1‑94 (MQ=60)
                                                                                 AACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATA           >  SRR4302532.671394/1‑94 (MQ=60)
                                                                                  ACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAC          <  SRR4302532.253319/94‑1 (MQ=60)
                                                                                   CTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT         >  SRR4302532.760174/1‑94 (MQ=60)
                                                                                   CTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT         >  SRR4302532.4057266/1‑94 (MQ=60)
                                                                                     TGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCA       >  SRR4302532.1967414/1‑94 (MQ=60)
                                                                                      GAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT      <  SRR4302532.3542656/94‑1 (MQ=60)
                                                                                      GAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT      <  SRR4302532.2153378/94‑1 (MQ=60)
                                                                                      GAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT      >  SRR4302532.5359085/1‑94 (MQ=60)
                                                                                      GAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT      <  SRR4302532.3465861/94‑1 (MQ=60)
                                                                                      GAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAT      >  SRR4302532.1578157/1‑94 (MQ=60)
                                                                                       AAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT     <  SRR4302532.1793403/94‑1 (MQ=60)
                                                                                       AAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT     <  SRR4302532.1854906/94‑1 (MQ=60)
                                                                                         GTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTT   <  SRR4302532.3145230/94‑1 (MQ=60)
                                                                                          TACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT  <  SRR4302532.3066603/94‑1 (MQ=60)
                                                                                             |                                                                                          
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT  >  NC_000913/4001624‑4001806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑