Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 593 264.2 13311096 97.8% 13018251 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,001,717 +C coding (86/903 nt) rarD ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,001,7171.C100.0% 839.5 / NA 235T29R (ACG→AGG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (99/136);  total (99/136)

Only 100 of 268 total aligned reads displayed.

TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT  >  NC_000913/4001625‑4001803
                                                                                             |                                                                                      
 gCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgc                                                                                        >  2:6006392/1‑92 (MQ=255)
 gCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgc                                                                                        >  1:3022695/1‑92 (MQ=255)
  cTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgct                                                                                       <  1:4005483/93‑3 (MQ=255)
    catcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgctgg                                                                                     <  1:3866257/93‑5 (MQ=255)
    catcaGCACCACCAGAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACgctgg                                                                                     <  2:5089863/93‑5 (MQ=255)
      tcaGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGgcg                                                                                   <  2:2132122/93‑1 (MQ=255)
       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGgcgc                                                                                  <  1:3848854/93‑1 (MQ=255)
       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGgcgc                                                                                  <  1:4676901/93‑1 (MQ=255)
       caGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATAAACTTGAAATACGCTGgcgc                                                                                  >  1:6013835/1‑93 (MQ=255)
        aGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCt                                                                                 <  1:553133/93‑1 (MQ=255)
         gCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCta                                                                                >  2:5952362/1‑93 (MQ=255)
           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCtata                                                                              <  1:5792123/93‑1 (MQ=255)
           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCtata                                                                              >  1:575009/1‑93 (MQ=255)
           accaccATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCtata                                                                              >  2:5826520/1‑93 (MQ=255)
               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAcccc                                                                          <  1:967746/93‑1 (MQ=255)
               ccaTAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATAcccc                                                                          <  1:4040926/93‑1 (MQ=255)
                  tAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaa                                                                       <  1:2642879/93‑1 (MQ=255)
                   aaaGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaat                                                                      <  1:3042430/93‑1 (MQ=255)
                     aGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataa                                                                    <  2:6438806/93‑1 (MQ=255)
                       aaaaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataaaa                                                                  <  2:173374/93‑1 (MQ=255)
                        aaaaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCaaataaaat                                                                 <  2:2032814/93‑1 (MQ=255)
                          aaCGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAaataaaataa                                                               <  1:2829718/93‑1 (MQ=255)
                           actaCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAg                                                              <  2:1282604/90‑1 (MQ=255)
                            cGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCGCGTAGTAAATCAACTTGGAGTACGCGGGGGCTATACCCCAAATAAAATAAGc                                                             >  1:6161954/1‑93 (MQ=255)
                             gACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCg                                                            <  2:1272271/93‑1 (MQ=255)
                              aCCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCgg                                                           >  1:4797158/1‑93 (MQ=255)
                                cAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCa                                                         <  1:3257808/93‑1 (MQ=255)
                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                        <  2:147675/93‑1 (MQ=255)
                                 aGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCaa                                                        >  2:1586081/1‑93 (MQ=255)
                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  2:3199668/93‑1 (MQ=255)
                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  2:3594123/93‑1 (MQ=255)
                                   aTCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAaga                                                      <  2:4695513/93‑1 (MQ=255)
                                    tCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAagag                                                     <  2:808979/93‑1 (MQ=255)
                                     cACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGc                                                    <  2:2060021/93‑1 (MQ=255)
                                      ccgcgATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCg                                                   <  2:6402533/92‑1 (MQ=255)
                                          gATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTa                                               >  2:3265655/1‑93 (MQ=255)
                                           aTGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTaa                                              <  1:1152483/93‑1 (MQ=255)
                                            tGCGTCAGGATTTCATCGGTGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAt                                             >  2:2397743/1‑93 (MQ=255)
                                              cGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAc                                           <  2:1032689/93‑1 (MQ=255)
                                               gTCAGGATTTCATCGGCGGGCACGGAGGAAATCAACTTGAAGGACGCTGGCGCCAAACCCCAAATAAAAAAAGCGGCAAGAGCGAGGAATACg                                          >  1:6648787/1‑93 (MQ=255)
                                                tCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCCAATAAAATAAGCGGCAAGAGCGAGGAATACGc                                         >  2:1192895/1‑93 (MQ=255)
                                                 cAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGcc                                        >  1:3789275/1‑93 (MQ=255)
                                                 cAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGGAATACGcc                                        >  2:5808694/1‑93 (MQ=255)
                                                  aGGATTTCATCGGCGGGCCCGTAGTAAATCAACTTGAAGTACGCTTGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                       >  1:6388921/1‑93 (MQ=255)
                                                  aGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGccc                                       >  2:5177104/1‑93 (MQ=255)
                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                      <  2:4973453/93‑1 (MQ=255)
                                                   ggATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCt                                      >  1:2799316/1‑93 (MQ=255)
                                                     aTTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGc                                    <  2:592579/93‑1 (MQ=255)
                                                      tttCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGAGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGcc                                   <  2:3802390/93‑1 (MQ=255)
                                                       ttCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcg                                  >  1:6073560/1‑93 (MQ=255)
                                                        tCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCcgc                                 <  1:1671604/93‑1 (MQ=255)
                                                          aTCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                               <  2:6036497/93‑1 (MQ=255)
                                                          aTCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                               <  1:4642818/93‑1 (MQ=255)
                                                          aTCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgt                               >  1:277137/1‑93 (MQ=255)
                                                           tCGGCGGGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtt                              <  1:3211520/93‑1 (MQ=255)
                                                             ggcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttg                            <  1:6374690/93‑1 (MQ=255)
                                                             ggcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttg                            >  1:1229211/1‑93 (MQ=255)
                                                             ggcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttg                            >  2:463803/1‑93 (MQ=255)
                                                              gcgtGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt                           <  1:1127567/93‑1 (MQ=255)
                                                              gcggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgt                           >  1:5675316/1‑93 (MQ=255)
                                                               cggGCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgtt                          <  1:5843671/93‑1 (MQ=255)
                                                                gggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCCATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCgtttgttt                         >  1:4861716/1‑93 (MQ=255)
                                                                 ggCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGtttt                        <  1:4612862/93‑1 (MQ=255)
                                                                  gCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTg                       <  2:151125/93‑1 (MQ=255)
                                                                  gCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTg                       >  2:6162407/1‑93 (MQ=255)
                                                                  gCACGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTg                       <  1:4844876/93‑1 (MQ=255)
                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  1:5191722/1‑93 (MQ=255)
                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     <  2:2861472/93‑1 (MQ=255)
                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  1:4993702/1‑93 (MQ=255)
                                                                    aCGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGca                     >  2:1625015/1‑93 (MQ=255)
                                                                     cGTAGTAAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcat                    >  1:5539928/1‑93 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc                   <  1:5396929/93‑1 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc                   >  2:402553/1‑93 (MQ=255)
                                                                      gtagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatc                   <  2:6312115/93‑1 (MQ=255)
                                                                       tagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcc                  >  1:829452/1‑93 (MQ=255)
                                                                       tagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcc                  >  1:1378664/1‑93 (MQ=255)
                                                                       tagtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcc                  <  2:4223102/93‑1 (MQ=255)
                                                                        agtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                 <  2:33436/93‑1 (MQ=255)
                                                                        agtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatcca                 <  2:5656368/93‑1 (MQ=255)
                                                                         gtaAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccat                <  1:2065918/93‑1 (MQ=255)
                                                                          taAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc               <  1:4441194/93‑1 (MQ=255)
                                                                          taAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc               <  1:3461660/93‑1 (MQ=255)
                                                                          taAATCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGcatccatc               <  1:2491843/93‑1 (MQ=255)
                                                                             aTCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCggg            >  2:1801051/1‑93 (MQ=255)
                                                                              tCAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGa           >  2:4599218/1‑93 (MQ=255)
                                                                               cAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGaa          >  1:6495402/1‑93 (MQ=255)
                                                                               cAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGaa          <  1:7771/93‑1 (MQ=255)
                                                                               cAACTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGaa          <  2:3453651/93‑1 (MQ=255)
                                                                                aaCTTGAAGTACGCTGGCGCTATTCCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGAGTTTGTTTTGCATCCATCGGGAAt         <  2:2543781/93‑1 (MQ=255)
                                                                                aaCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAt         <  2:6582050/93‑1 (MQ=255)
                                                                                 aCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATa        <  2:4434025/93‑1 (MQ=255)
                                                                                 aCTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATa        <  2:1911493/93‑1 (MQ=255)
                                                                                  cTTGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAc       <  2:3400225/93‑1 (MQ=255)
                                                                                   ttGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACt      >  1:2319311/1‑93 (MQ=255)
                                                                                    tGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTc     <  1:4263914/93‑1 (MQ=255)
                                                                                    tGAAGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTc     >  1:3714754/1‑93 (MQ=255)
                                                                                      aaGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt   >  2:5426871/1‑93 (MQ=255)
                                                                                      aaGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt   <  1:68126/93‑1 (MQ=255)
                                                                                      aaGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAt   >  2:679761/1‑93 (MQ=255)
                                                                                       aGTACGCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCAtt  <  2:5399418/93‑1 (MQ=255)
                                                                                             |                                                                                      
TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACG‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT  >  NC_000913/4001625‑4001803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT  >  NC_000913/4001624‑4001806
                                                                                              |                                                                                          
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G                                                                                            <  SRR4302547.4564437/94‑1 (MQ=60)
 TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCCCGTAGTAAATCAACTTGAAGTAC‑G‑C                                                                                          >  SRR4302547.945476/1‑94 (MQ=60)
 TGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑C                                                                                          >  SRR4302547.3082803/1‑94 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gct                                                                                          >  SRR4302547.6166744/1‑92 (MQ=60)
  GCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gct                                                                                          <  SRR4302547.785732/94‑3 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctg                                                                                         <  SRR4302547.2867987/94‑4 (MQ=60)
   CTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctg                                                                                         <  SRR4302547.4086894/94‑4 (MQ=60)
    TCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGG                                                                                        <  SRR4302547.4101916/94‑1 (MQ=60)
    TCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGG                                                                                        <  SRR4302547.233421/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctggg                                                                                       >  SRR4302547.3070383/1‑89 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGC                                                                                       <  SRR4302547.2961104/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGC                                                                                       <  SRR4302547.3944953/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGC                                                                                       <  SRR4302547.3944952/94‑1 (MQ=60)
     CATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTAC‑GCTGGC                                                                                       >  SRR4302547.4757102/1‑94 (MQ=60)
       TCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATAAACTTGAAATAC‑GCTGGCGC                                                                                     >  SRR4302547.6138649/1‑94 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑Gctggcggt                                                                                    >  SRR4302547.6694871/1‑86 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCT                                                                                    <  SRR4302547.4773777/94‑1 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCT                                                                                    <  SRR4302547.3927210/94‑1 (MQ=60)
        CAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAA‑GCTGGCGCT                                                                                    >  SRR4302547.3730310/1‑94 (MQ=60)
         AGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTA                                                                                   >  SRR4302547.6487232/1‑94 (MQ=60)
         AGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTA                                                                                   <  SRR4302547.563096/94‑1 (MQ=60)
          GCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTAT                                                                                  >  SRR4302547.1181690/1‑94 (MQ=60)
           CACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATA                                                                                 >  SRR4302547.585323/1‑94 (MQ=60)
            ACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAC                                                                                <  SRR4302547.5911929/94‑1 (MQ=60)
                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCA                                                                            >  SRR4302547.4022111/1‑94 (MQ=60)
                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCA                                                                            <  SRR4302547.4122991/94‑1 (MQ=60)
                CCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCA                                                                            <  SRR4302547.986535/94‑1 (MQ=60)
                  ATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAA                                                                          >  SRR4302547.3939645/1‑94 (MQ=60)
                  ATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAA                                                                          >  SRR4302547.5584855/1‑94 (MQ=60)
                  ATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAA                                                                          <  SRR4302547.5995128/94‑1 (MQ=60)
                   TAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAAT                                                                         >  SRR4302547.1220664/1‑94 (MQ=60)
                   TAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAAT                                                                         <  SRR4302547.2696068/94‑1 (MQ=60)
                   TAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATAACCCAAAT                                                                         >  SRR4302547.3182415/1‑94 (MQ=60)
                    AAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATA                                                                        <  SRR4302547.3102981/94‑1 (MQ=60)
                     AAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAA                                                                       <  SRR4302547.2582279/94‑1 (MQ=60)
                      AGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTCAAGTAC‑GCTGGCGCTATACCCCAAATAAA                                                                      >  SRR4302547.593798/1‑94 (MQ=60)
                       GAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTACAC‑TGGCGCTATACCCCAAATAAAA                                                                     <  SRR4302547.4841420/94‑1 (MQ=60)
                           AACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAG                                                                 <  SRR4302547.2886302/94‑1 (MQ=60)
                            ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCGCGTAGTAAATCAACTTGGAGTAC‑GCGGGGGCTATACCCCAAATAAAATAAGC                                                                >  SRR4302547.6289835/1‑94 (MQ=60)
                            ACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGC                                                                <  SRR4302547.3095390/94‑1 (MQ=60)
                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCG                                                               <  SRR4302547.2731125/94‑1 (MQ=60)
                             CGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCG                                                               <  SRR4302547.4414305/94‑1 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGG                                                              >  SRR4302547.4896562/1‑94 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGG                                                              >  SRR4302547.4707665/1‑94 (MQ=60)
                              GACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGG                                                              >  SRR4302547.1792695/1‑94 (MQ=60)
                               ACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGC                                                             >  SRR4302547.4024239/1‑94 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAA                                                           <  SRR4302547.3322913/94‑1 (MQ=60)
                                 CAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAACGGCAA                                                           >  SRR4302547.1299645/1‑94 (MQ=60)
                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                          >  SRR4302547.3918273/1‑94 (MQ=60)
                                  AGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAG                                                          <  SRR4302547.5557864/94‑1 (MQ=60)
                                    ATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAG                                                        >  SRR4302547.2828818/1‑94 (MQ=60)
                                     TCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGC                                                       <  SRR4302547.5395584/94‑1 (MQ=60)
                                     TCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGC                                                       <  SRR4302547.4715688/94‑1 (MQ=60)
                                      CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCG                                                      >  SRR4302547.2255990/1‑94 (MQ=60)
                                      CACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAAATTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAAAAAGCGGCAAGAGCG                                                      >  SRR4302547.6520122/1‑94 (MQ=60)
                                       ACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGG                                                     >  SRR4302547.800991/1‑94 (MQ=60)
                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATATCCCAAATAAAATAAagcgcaaaaacgaga                                                   >  SRR4302547.6764523/1‑79 (MQ=60)
                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGT                                                   >  SRR4302547.6077117/1‑94 (MQ=60)
                                         GCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGA                                                   >  SRR4302547.1873669/1‑94 (MQ=60)
                                          CGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTA                                                  >  SRR4302547.4510144/1‑94 (MQ=60)
                                           GATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTTAAGTAC‑GCTGGCGCTATAACCCCAATAAAATAAGCCGCAAGAGCGcggaa                                                 >  SRR4302547.4643626/1‑89 (MQ=60)
                                            ATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAT                                                <  SRR4302547.1175812/94‑1 (MQ=60)
                                            ATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAAT                                                <  SRR4302547.5022160/94‑1 (MQ=60)
                                              GCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAC                                              <  SRR4302547.4883167/94‑1 (MQ=60)
                                               CGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACG                                             <  SRR4302547.3611496/94‑1 (MQ=60)
                                               CGTCAGGATTTCATCGGCGGGCACGGAGGAAATCAACTTGAAGGAC‑GCTGGCGCCAAACCCCAAATAAAAAAAGCGGCAAGAGCGAGGAATACG                                             >  SRR4302547.6787293/1‑94 (MQ=57)
                                                GTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGC                                            <  SRR4302547.3296356/94‑1 (MQ=60)
                                                 TCAGGATTTCATCGGCGGGCCCGTAGTAAAACAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCACGGGCGAGTAATGCGCC                                           >  SRR4302547.1989833/1‑94 (MQ=60)
                                                 TCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCC                                           >  SRR4302547.3866534/1‑94 (MQ=60)
                                                 TCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCC                                           <  SRR4302547.3147538/94‑1 (MQ=60)
                                                  CAGGATTTCATCGGCGGGCCCGTAGTAAATCAACTTGAAGTAC‑GCTTGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCC                                          >  SRR4302547.6522225/1‑94 (MQ=60)
                                                  CAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCC                                          <  SRR4302547.4947273/94‑1 (MQ=60)
                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                         >  SRR4302547.5648862/1‑94 (MQ=60)
                                                   AGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCT                                         >  SRR4302547.2855367/1‑94 (MQ=60)
                                                     GATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATAAGCCCTGC                                       >  SRR4302547.580776/1‑94 (MQ=60)
                                                       TTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCG                                     >  SRR4302547.6199557/1‑94 (MQ=60)
                                                         TCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCG                                   <  SRR4302547.1704535/94‑1 (MQ=60)
                                                         TCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCG                                   >  SRR4302547.499310/1‑94 (MQ=60)
                                                          CATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGT                                  >  SRR4302547.282310/1‑94 (MQ=60)
                                                           ATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                 >  SRR4302547.2482747/1‑94 (MQ=60)
                                                           ATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTT                                 <  SRR4302547.4738976/94‑1 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                <  SRR4302547.4260083/94‑1 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                >  SRR4302547.5164302/1‑94 (MQ=60)
                                                            TCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTT                                <  SRR4302547.3275587/94‑1 (MQ=60)
                                                             CGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTG                               >  SRR4302547.1254010/1‑94 (MQ=60)
                                                              GGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT                              <  SRR4302547.6507690/94‑1 (MQ=60)
                                                              GGCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGT                              >  SRR4302547.5792501/1‑94 (MQ=60)
                                                               GCGTGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                             <  SRR4302547.1150321/94‑1 (MQ=60)
                                                               GCGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTT                             <  SRR4302547.6599160/94‑1 (MQ=60)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                            <  SRR4302547.5964558/94‑1 (MQ=60)
                                                                CGGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCCATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTT                            >  SRR4302547.4962461/1‑94 (MQ=60)
                                                                 GGGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTT                           >  SRR4302547.3858261/1‑94 (MQ=60)
                                                                  GGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTG                          <  SRR4302547.4708348/94‑1 (MQ=60)
                                                                  GGCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTG                          <  SRR4302547.3813950/94‑1 (MQ=60)
                                                                   GCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGC                         <  SRR4302547.4945275/94‑1 (MQ=60)
                                                                   GCACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGC                         >  SRR4302547.4918619/1‑94 (MQ=60)
                                                                    CACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                        >  SRR4302547.5298572/1‑94 (MQ=60)
                                                                    CACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCA                        >  SRR4302547.5096944/1‑94 (MQ=60)
                                                                     ACGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCAT                       >  SRR4302547.5654266/1‑94 (MQ=60)
                                                                      CGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATC                      >  SRR4302547.6769201/1‑94 (MQ=60)
                                                                      CGTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGGTTGTTTTGCATC                      >  SRR4302547.4675547/1‑94 (MQ=60)
                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                     >  SRR4302547.1406302/1‑94 (MQ=60)
                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                     >  SRR4302547.3380469/1‑94 (MQ=60)
                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                     >  SRR4302547.845290/1‑94 (MQ=60)
                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCC                     <  SRR4302547.5508433/94‑1 (MQ=60)
                                                                       GTAGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCAGCGTTTGTTTTGCATCC                     >  SRR4302547.852464/1‑94 (MQ=60)
                                                                         AGTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCAT                   >  SRR4302547.5406676/1‑94 (MQ=60)
                                                                          GTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                  <  SRR4302547.1528572/94‑1 (MQ=60)
                                                                          GTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATC                  <  SRR4302547.2107142/94‑1 (MQ=60)
                                                                          GTAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATA                  <  SRR4302547.6208679/94‑1 (MQ=60)
                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 <  SRR4302547.5853754/94‑1 (MQ=60)
                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 <  SRR4302547.695382/94‑1 (MQ=60)
                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 <  SRR4302547.4533024/94‑1 (MQ=60)
                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 <  SRR4302547.2542175/94‑1 (MQ=60)
                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 <  SRR4302547.3532252/94‑1 (MQ=60)
                                                                           TAAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 <  SRR4302547.6361644/94‑1 (MQ=60)
                                                                           TAAATCAACTTAAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCG                 >  SRR4302547.4762033/1‑94 (MQ=60)
                                                                            AAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                <  SRR4302547.820175/94‑1 (MQ=60)
                                                                            AAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                <  SRR4302547.1156518/94‑1 (MQ=60)
                                                                            AAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                >  SRR4302547.5701703/1‑94 (MQ=60)
                                                                            AAATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGG                >  SRR4302547.494094/1‑94 (MQ=60)
                                                                             AATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGG               <  SRR4302547.2890909/94‑1 (MQ=60)
                                                                             AATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGG               <  SRR4302547.4322208/94‑1 (MQ=60)
                                                                              ATCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGA              >  SRR4302547.3295002/1‑94 (MQ=60)
                                                                               TCAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAA             >  SRR4302547.6630611/1‑94 (MQ=60)
                                                                                CAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAT            <  SRR4302547.7918/94‑1 (MQ=60)
                                                                                CAACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAT            <  SRR4302547.2556098/94‑1 (MQ=60)
                                                                                CAACTTGAAGTAC‑GCCGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAAT            <  SRR4302547.4746856/94‑1 (MQ=60)
                                                                                  ACTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATAC          <  SRR4302547.621017/94‑1 (MQ=60)
                                                                                   CTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT         <  SRR4302547.5931297/94‑1 (MQ=60)
                                                                                   CTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT         >  SRR4302547.2366313/1‑94 (MQ=60)
                                                                                   CTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT         >  SRR4302547.5897961/1‑94 (MQ=60)
                                                                                   CTTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACT         <  SRR4302547.1289435/94‑1 (MQ=60)
                                                                                    TTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTC        >  SRR4302547.3790580/1‑94 (MQ=60)
                                                                                    TTGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTC        <  SRR4302547.5722055/94‑1 (MQ=60)
                                                                                     TGAAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCA       <  SRR4302547.4351217/94‑1 (MQ=60)
                                                                                       AAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT     <  SRR4302547.691200/94‑1 (MQ=60)
                                                                                       AAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT     <  SRR4302547.69401/94‑1 (MQ=60)
                                                                                       AAGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATT     <  SRR4302547.2880055/94‑1 (MQ=60)
                                                                                        AGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTT    >  SRR4302547.1478060/1‑94 (MQ=60)
                                                                                        AGTAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTT    <  SRR4302547.6571281/94‑1 (MQ=60)
                                                                                          TAC‑GCTGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT  >  SRR4302547.5875153/1‑94 (MQ=60)
                                                                                              |                                                                                          
ATGCTCATCAGCACCACCATAAAGAAAAACGACCAGATCACGCGATGCGTCAGGATTTCATCGGCGGGCACGTAGTAAATCAACTTGAAGTAC‑G‑TGGCGCTATACCCCAAATAAAATAAGCGGCAAGAGCGAGTAATACGCCCTGCCGCGTTTGTTTTGCATCCATCGGGAATACTCATTTTT  >  NC_000913/4001624‑4001806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑