Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,004,979	+G	coding (1511/1833 nt)	recQ →	ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,004,979	1	.	G	99.5%	681.2 / ‑4.3	189	coding (1511/1833 nt)	recQ	ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (97/91); total (97/92)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060

cGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCt                                                                                      <  1:1060373/93‑1 (MQ=255)

cGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCt                                                                                      <  2:2698583/93‑1 (MQ=255)

cGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCt                                                                                      <  2:143774/93‑1 (MQ=255)

 gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCg‑‑c‑tgctg                                                                                     >  2:2839820/1‑92 (MQ=255)

  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCg‑‑c‑tgctgc                                                                                    >  2:2126643/1‑91 (MQ=255)

  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCtgc                                                                                    <  2:4813092/93‑3 (MQ=255)

  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCtgc                                                                                    >  1:295323/1‑91 (MQ=255)

    aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCtgcgc                                                                                  <  2:2631111/93‑5 (MQ=255)

        gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcg                                                                            >  1:4798325/1‑91 (MQ=255)

        cccAGCTGATTCACCTCGGCCTGGTGACGAAAAATATTGCGCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCg‑‑gcg                                                                            <  2:2570608/92‑3 (MQ=255)

                aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCctct                                                                      >  2:5530869/1‑93 (MQ=255)

                aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCctct                                                                      <  1:609470/93‑1 (MQ=255)

                 ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCtt                                                                     >  1:5648801/1‑93 (MQ=255)

                  tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCttt                                                                    <  1:3078095/93‑1 (MQ=255)

                  tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCttt                                                                    <  2:4258183/93‑1 (MQ=255)

                   cACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTg                                                                   <  1:3949835/93‑1 (MQ=255)

                    cccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGc                                                                  <  1:1881852/92‑1 (MQ=255)

                    cccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGc                                                                  <  2:1764047/92‑1 (MQ=255)

                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  2:5626980/1‑93 (MQ=255)

                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  2:2649514/1‑93 (MQ=255)

                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAAc                                                               >  2:647907/1‑93 (MQ=255)

                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                            >  2:4415834/1‑93 (MQ=255)

                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                            <  1:4888766/93‑1 (MQ=255)

                           ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgc                                                           <  2:3026203/93‑1 (MQ=255)

                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          <  1:2319468/93‑1 (MQ=255)

                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          >  2:1945712/1‑93 (MQ=255)

                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                          <  1:3721942/93‑1 (MQ=255)

                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         >  1:4225600/1‑93 (MQ=255)

                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                         <  1:1366513/93‑1 (MQ=255)

                              ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                        <  2:3845482/93‑1 (MQ=255)

                              ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                        <  2:5968971/93‑1 (MQ=255)

                                tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgc                                                      >  1:1559833/1‑93 (MQ=255)

                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                     >  1:737909/1‑93 (MQ=255)

                                     cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt                                                 >  1:2814729/1‑93 (MQ=255)

                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:1683698/1‑93 (MQ=255)

                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:1170528/1‑93 (MQ=255)

                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:2370855/1‑93 (MQ=255)

                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                <  1:3630741/93‑1 (MQ=255)

                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  2:4664803/1‑93 (MQ=255)

                                       aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  2:3489286/93‑1 (MQ=255)

                                       aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               >  2:3414661/1‑93 (MQ=255)

                                        aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  2:4431861/93‑1 (MQ=255)

                                         atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             <  2:3083277/93‑1 (MQ=255)

                                         atatTCCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             <  1:2727634/93‑1 (MQ=255)

                                           atTGCCCAGCATTGTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  2:498025/93‑1 (MQ=255)

                                           atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTCTCGTg                                           >  2:6066578/1‑93 (MQ=255)

                                           atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  1:4753517/93‑1 (MQ=255)

                                           atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGAATCGTg                                           >  1:1030131/1‑93 (MQ=255)

                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          <  2:1037462/93‑1 (MQ=255)

                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          <  1:5049261/93‑1 (MQ=255)

                                             tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:68579/93‑1 (MQ=255)

                                                ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      <  1:3289315/93‑1 (MQ=255)

                                                ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:5797120/1‑93 (MQ=255)

                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:42412/1‑93 (MQ=255)

                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:1363505/1‑93 (MQ=255)

                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  1:4655796/1‑93 (MQ=255)

                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:3145927/1‑93 (MQ=255)

                                                   gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  1:4998299/1‑93 (MQ=255)

                                                    cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  <  1:5977428/93‑1 (MQ=255)

                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  1:5450267/1‑93 (MQ=255)

                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  1:337056/1‑93 (MQ=255)

                                                     aTTCTGCCATACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  1:1477040/93‑1 (MQ=255)

                                                        cTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              >  2:4790687/1‑93 (MQ=255)

                                                         tGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             <  2:4809197/93‑1 (MQ=255)

                                                         tGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  2:3797509/1‑93 (MQ=255)

                                                           cccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  1:5771225/93‑1 (MQ=255)

                                                            ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  2:5234938/1‑93 (MQ=255)

                                                            ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  2:368662/1‑93 (MQ=255)

                                                            ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:892257/1‑93 (MQ=255)

                                                            ccTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:2440838/1‑93 (MQ=255)

                                                             cTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGTCGCTCAAACCGAAAGCg                         >  2:201437/1‑93 (MQ=255)

                                                             cTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         >  1:2544557/1‑93 (MQ=255)

                                                             cTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  1:6040366/93‑1 (MQ=255)

                                                              tACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:1985263/1‑93 (MQ=255)

                                                              tACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:1510820/1‑93 (MQ=255)

                                                              tACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  2:2192508/93‑1 (MQ=255)

                                                               aCAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       >  1:1690995/1‑93 (MQ=255)

                                                                cAACTGGCAGAGGCCGCGCGCCCGATGCTGCT‑CGGC‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATg                      <  2:2111542/93‑1 (MQ=255)

                                                                 aaCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     >  2:5389368/1‑93 (MQ=255)

                                                                 aaCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  2:1693106/93‑1 (MQ=255)

                                                                 aaCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  1:5357166/93‑1 (MQ=255)

                                                                  aCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  1:5131318/1‑93 (MQ=255)

                                                                  aCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  1:1233682/93‑1 (MQ=255)

                                                                  aCTGACAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  2:3896197/93‑1 (MQ=255)

                                                                       cAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  1:342652/93‑1 (MQ=255)

                                                                       cAGAGGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  2:1231476/93‑1 (MQ=255)

                                                                         gagGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  2:2973763/1‑93 (MQ=255)

                                                                          agGCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            <  1:4044773/93‑1 (MQ=255)

                                                                           ggCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  1:3896281/93‑1 (MQ=255)

                                                                           ggCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:3343673/93‑1 (MQ=255)

                                                                            gCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:6071625/93‑1 (MQ=255)

                                                                            gCCGCGCGCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:5869253/93‑1 (MQ=255)

                                                                              cgcgcgCCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg        >  2:4038532/1‑93 (MQ=255)

                                                                               gcgcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:315475/1‑93 (MQ=255)

                                                                               gcgcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:1830231/93‑1 (MQ=255)

                                                                                cgcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:6141102/93‑1 (MQ=255)

                                                                                cgcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      >  1:4459486/1‑93 (MQ=255)

                                                                                 gcgcCCG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg     <  1:4621932/93‑1 (MQ=255)

                                                                                    cccG‑‑G‑TGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  <  2:3742105/93‑1 (MQ=255)

                                                                                    cccG‑‑G‑TGCTGCGCGGCGAATCCTATTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  2:5425263/1‑93 (MQ=255)

CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCG‑‑G‑TGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063

GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T                                                                                           <  SRR4302532.3345422/94‑1 (MQ=60)

GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T                                                                                           <  SRR4302532.5580751/94‑1 (MQ=60)

GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T                                                                                           <  SRR4302532.4576651/94‑1 (MQ=60)

 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑G                                                                                       <  SRR4302532.947116/94‑1 (MQ=60)

 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑G                                                                                       <  SRR4302532.1096090/94‑1 (MQ=60)

  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgc                                                                                         >  SRR4302532.305508/1‑92 (MQ=60)

   TGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑‑GCG                                                                                    >  SRR4302532.1612025/1‑94 (MQ=60)

       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggc                                                                                    >  SRR4302532.2328261/1‑87 (MQ=60)

        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcg                                                                                   <  SRR4302532.1473149/94‑9 (MQ=60)

        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcg                                                                                   >  SRR4302532.4963583/1‑86 (MQ=60)

        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcg                                                                                   >  SRR4302532.5218456/1‑86 (MQ=60)

        CGCCAGCTGATTCACCTCGGCCTGGTAACGCAAAATATTGCCCAGAATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcg                                                                                   <  SRR4302532.4910769/94‑9 (MQ=60)

        CCCCAGCTGATTCCCCGCGGCCTGGTGACGCAAAATATTCCCCAGCATTCTGCCTTACAACTGCCAGGGCCCGCGCGCCCGGTGC‑Tgcgcggcg                                                                                   <  SRR4302532.1514566/94‑9 (MQ=57)

         GCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAAGTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcga                                                                                  >  SRR4302532.5327116/1‑85 (MQ=60)

         GCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcga                                                                                  >  SRR4302532.1756029/1‑85 (MQ=60)

          CCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGACCTACAACTGACAGAGACCGCGCGCCCGGTGC‑Tgcgcggcgaa                                                                                 >  SRR4302532.1179695/1‑84 (MQ=60)

           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaat                                                                                <  SRR4302532.6288835/94‑12 (MQ=60)

           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaat                                                                                >  SRR4302532.2539171/1‑83 (MQ=60)

            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaatc                                                                               >  SRR4302532.3830358/1‑82 (MQ=60)

            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaatc                                                                               <  SRR4302532.2195777/94‑13 (MQ=60)

             gcttgttccacctcGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaatcc                                                                              <  SRR4302532.2765530/80‑14 (MQ=60)

              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCT                                                                           >  SRR4302532.4826974/1‑94 (MQ=60)

               TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTC                                                                          >  SRR4302532.271349/1‑94 (MQ=60)

                 ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTT                                                                        <  SRR4302532.630719/94‑1 (MQ=60)

                 ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTT                                                                        >  SRR4302532.5845324/1‑94 (MQ=60)

                  TTCACCTCGGCCGGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTT                                                                       <  SRR4302532.4050680/94‑1 (MQ=60)

                   TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTG                                                                      <  SRR4302532.3179821/94‑1 (MQ=60)

                    CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGC                                                                     <  SRR4302532.4082946/94‑1 (MQ=60)

                     CCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCA                                                                    <  SRR4302532.1944241/94‑1 (MQ=60)

                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACT                                                                 <  SRR4302532.2931101/94‑1 (MQ=60)

                         CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTT                                                                <  SRR4302532.502225/94‑1 (MQ=60)

                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTG                                                               <  SRR4302532.2518226/94‑1 (MQ=60)

                           GCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGC                                                              <  SRR4302532.5056790/94‑1 (MQ=60)

                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCC                                                             >  SRR4302532.2642310/1‑94 (MQ=60)

                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCG                                                            <  SRR4302532.2396662/94‑1 (MQ=60)

                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCG                                                            <  SRR4302532.3846914/94‑1 (MQ=60)

                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCG                                                            >  SRR4302532.4367436/1‑94 (MQ=60)

                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGT                                                           <  SRR4302532.1412277/94‑1 (MQ=60)

                               GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                          >  SRR4302532.5860210/1‑94 (MQ=60)

                                GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGC                                                         >  SRR4302532.1612587/1‑94 (MQ=60)

                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302532.763042/1‑94 (MQ=60)

                                     GCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGT                                                    >  SRR4302532.2908941/1‑94 (MQ=60)

                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   >  SRR4302532.1293676/1‑94 (MQ=60)

                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   >  SRR4302532.495549/1‑94 (MQ=60)

                                       AAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAT                                                  <  SRR4302532.3752521/94‑1 (MQ=60)

                                         AATATTGCTGAGCATTCTGCCCTACAACTGACAGAGGCAGCGCGCCAGGTGC‑TGGCCGCC‑‑GAATCCTCTTTGCATCTTGCCGTGCCGCGGATCG                                                <  SRR4302532.2848008/94‑1 (MQ=48)

                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302532.5804768/1‑94 (MQ=60)

                                          ATATTCCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               <  SRR4302532.2819346/94‑1 (MQ=60)

                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              >  SRR4302532.4248085/1‑94 (MQ=60)

                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGAATCGTG                                              >  SRR4302532.1064936/1‑94 (MQ=60)

                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302532.4916073/94‑1 (MQ=60)

                                             TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                            <  SRR4302532.5222519/94‑1 (MQ=60)

                                               GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                          >  SRR4302532.5154066/1‑94 (MQ=60)

                                               GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                          >  SRR4302532.1965483/1‑94 (MQ=60)

                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302532.4472379/1‑94 (MQ=60)

                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        <  SRR4302532.3398737/94‑1 (MQ=60)

                                                  CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       >  SRR4302532.4814527/1‑94 (MQ=60)

                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302532.5169634/1‑94 (MQ=60)

                                                    GCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAA                                     >  SRR4302532.917104/1‑94 (MQ=60)

                                                    CCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGAGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCccgga                                     >  SRR4302532.307188/1‑89 (MQ=60)

                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302532.3453906/94‑1 (MQ=60)

                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302532.5639170/1‑94 (MQ=60)

                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302532.6184021/94‑1 (MQ=60)

                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302532.6184022/94‑1 (MQ=60)

                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302532.348731/1‑94 (MQ=60)

                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302532.2879525/1‑94 (MQ=60)

                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302532.6313159/1‑94 (MQ=60)

                                                      ATTCTGCCATACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302532.1526821/94‑1 (MQ=60)

                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  >  SRR4302532.5990555/1‑94 (MQ=60)

                                                        TCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGA                                 <  SRR4302532.4682352/94‑1 (MQ=60)

                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302532.5972042/94‑1 (MQ=60)

                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302532.922726/1‑94 (MQ=60)

                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302532.2521993/1‑94 (MQ=60)

                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            <  SRR4302532.4803146/94‑1 (MQ=60)

                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302532.2629425/1‑94 (MQ=60)

                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302532.3849496/1‑94 (MQ=60)

                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           <  SRR4302532.6248962/94‑1 (MQ=60)

                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302532.1561836/1‑94 (MQ=60)

                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302532.2051212/1‑94 (MQ=60)

                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          >  SRR4302532.1748103/1‑94 (MQ=60)

                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302532.4309249/94‑1 (MQ=60)

                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         >  SRR4302532.3086317/1‑94 (MQ=60)

                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302532.5307841/1‑94 (MQ=60)

                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302532.5542928/94‑1 (MQ=60)

                                                                   ACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      <  SRR4302532.1274958/94‑1 (MQ=60)

                                                                   ACTGACAGAGGCCGCCCCCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCGGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      <  SRR4302532.1551755/94‑1 (MQ=60)

                                                                      GACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAATGCGATGCAGAAA                   <  SRR4302532.3809417/94‑1 (MQ=60)

                                                                       ACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  <  SRR4302532.3235216/94‑1 (MQ=60)

                                                                        CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302532.3744020/94‑1 (MQ=60)

                                                                        CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302532.354534/94‑1 (MQ=60)

                                                                         AGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302532.4130855/1‑94 (MQ=60)

                                                                          cctGCCGCGCGCCCGGTGCGG‑‑‑CGCGGCGAATCCTCTTTGCAACTTCCCGTGCCGCGTATCGTGGCCCTCAATCCGAAAGCCATGCAGCCATCGT               <  SRR4302532.434318/91‑1 (MQ=37)

                                                                          GAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               <  SRR4302532.6277042/94‑1 (MQ=60)

                                                                           AGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              <  SRR4302532.4180957/94‑1 (MQ=60)

                                                                            GGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302532.4027711/94‑1 (MQ=60)

                                                                            GGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302532.5256968/94‑1 (MQ=60)

                                                                             GCCGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302532.6281310/94‑1 (MQ=60)

                                                                               CGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          <  SRR4302532.1687164/94‑1 (MQ=60)

                                                                               CGCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          >  SRR4302532.717551/1‑94 (MQ=60)

                                                                                GCGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         >  SRR4302532.4611505/1‑94 (MQ=60)

                                                                                 CGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302532.6352941/94‑1 (MQ=60)

                                                                                 CGCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        >  SRR4302532.3525170/1‑94 (MQ=60)

                                                                                  GCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       <  SRR4302532.4779535/94‑1 (MQ=60)

                                                                                  GCGCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGA       >  SRR4302532.2583901/1‑94 (MQ=60)

                                                                                    GCCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     >  SRR4302532.1081302/1‑94 (MQ=60)

                                                                                     CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    <  SRR4302532.5594246/94‑1 (MQ=60)

                                                                                     CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    >  SRR4302532.5024543/1‑94 (MQ=60)

                                                                                     CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    >  SRR4302532.2738554/1‑94 (MQ=60)

                                                                                     CCCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCAGCAAC    >  SRR4302532.2738553/1‑94 (MQ=60)

                                                                                      CCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   <  SRR4302532.2422984/94‑1 (MQ=60)

                                                                                      CCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   >  SRR4302532.992749/1‑94 (MQ=60)

                                                                                      CCGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATATATCGGCGGCAACT   >  SRR4302532.4610544/1‑94 (MQ=60)

                                                                                       CGGTGC‑TG‑‑CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  SRR4302532.362914/1‑94 (MQ=60)

GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`