Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,717	+G	coding (3/999 nt)	ysgA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,717	1	.	G	99.6%	801.6 / ‑3.8	224	coding (3/999 nt)	ysgA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (110/113); total (110/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 235 total aligned reads displayed.

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

tAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGc                                                                                          >  1:3539591/1‑93 (MQ=255)

  aGCAGGCTTTTTATCCCCTTGGGAAGGGATAGAGGTAAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcga                                                                                        <  1:1144476/93‑3 (MQ=255)

  aGCAGGCGTTTTATCCCTTTGTGAGGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcga                                                                                        <  1:1873916/93‑3 (MQ=255)

      ggCATTTTATCCCTTGGGGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                    <  2:3693112/93‑1 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGGGAGAGGCGATgca                                                                                    >  2:1215734/1‑93 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                    >  1:4631928/1‑93 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                    >  2:3791954/1‑93 (MQ=255)

      ggCATGTTACCCCCTTGTGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                    <  1:2459472/93‑1 (MQ=255)

         ttGTTCTCCCTTGGAGAAGGGATAGACGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcagca                                                                                 <  2:2113734/92‑1 (MQ=255)

          tGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                                >  1:5749522/1‑93 (MQ=255)

          tGTTACCCCTTTGTGAAGGGATAGAGGTGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                                <  2:2970761/93‑1 (MQ=255)

           tttACCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAgg                                                                               <  1:4913878/92‑1 (MQ=255)

            ttATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAggg                                                                              >  1:4683587/1‑92 (MQ=255)

            ttATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAggg                                                                              >  1:2220674/1‑92 (MQ=255)

            ttATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAggg                                                                              >  2:4623930/1‑92 (MQ=255)

            ttATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGt                                                                              >  2:2882001/1‑93 (MQ=255)

              aTCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGc                                                                            >  1:5625371/1‑93 (MQ=255)

               tCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCa                                                                           >  1:4403030/1‑93 (MQ=255)

                ccccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGCGGCGATGCAGCAGGTGCaa                                                                          >  2:1628863/1‑93 (MQ=255)

                  ccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAt                                                                        >  2:5246592/1‑93 (MQ=255)

                   tttGTAAAGGGAAAGGGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                       <  1:3634568/92‑1 (MQ=255)

                   cTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                       >  2:3446388/1‑93 (MQ=255)

                   cTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                       <  2:1843859/93‑1 (MQ=255)

                   cTTGTGAAGGGATAGAGGTAACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATc                                                                       <  2:878001/93‑1 (MQ=255)

                     tgtgAAGGGAGAGGTGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCa                                                                     <  1:6050650/93‑1 (MQ=255)

                       tgAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGa                                                                   >  1:5572591/1‑93 (MQ=255)

                        gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCACATCCAGAt                                                                  >  2:3266891/1‑93 (MQ=255)

                        gAAGGGAGAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                  <  1:5217518/93‑1 (MQ=255)

                         gaGGGAAAGGGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAtt                                                                 <  2:4320769/92‑1 (MQ=255)

                             gATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgttg                                                             <  1:889529/93‑1 (MQ=255)

                              aTAGAGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                            <  1:3864174/93‑1 (MQ=255)

                               tAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtg                                                           >  2:4530662/1‑93 (MQ=255)

                                 gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                         >  2:4248382/1‑93 (MQ=255)

                                 gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                         >  1:1331620/1‑93 (MQ=255)

                                  gggTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgttg                                                        <  1:176249/92‑1 (MQ=255)

                                  agGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgttg                                                        >  1:2245270/1‑93 (MQ=255)

                                    gTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTgttgtt                                                      <  1:292174/93‑1 (MQ=255)

                                     tGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGttgttg                                                     >  2:4065814/1‑93 (MQ=255)

                                      gAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                    >  2:2281885/1‑93 (MQ=255)

                                       aaGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                   >  2:2520187/1‑93 (MQ=255)

                                        aGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                  >  1:5388244/1‑93 (MQ=255)

                                          cccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATg                                                <  1:4508411/93‑1 (MQ=255)

                                          cccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATg                                                <  1:2875780/93‑1 (MQ=255)

                                           ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                               >  2:3634479/1‑93 (MQ=255)

                                           ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                               <  1:4723416/93‑1 (MQ=255)

                                           ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                               >  2:5663360/1‑93 (MQ=255)

                                            cGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGt                                              >  1:944145/1‑93 (MQ=255)

                                               cacaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAtt                                           >  2:4052053/1‑93 (MQ=255)

                                               cacaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAtt                                           >  1:2739907/1‑93 (MQ=255)

                                                acaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtc                                          >  1:5089787/1‑93 (MQ=255)

                                                acaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtc                                          >  1:1407728/1‑93 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                        >  2:3228433/1‑93 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                        >  1:3446248/1‑93 (MQ=255)

                                                    tCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCg                                      <  2:5093336/93‑1 (MQ=255)

                                                    tCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCg                                      <  2:96813/93‑1 (MQ=255)

                                                    tCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCg                                      <  2:6029825/93‑1 (MQ=255)

                                                      gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTa                                    <  2:2344027/93‑1 (MQ=255)

                                                        gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                 >  1:1027680/1‑93 (MQ=255)

                                                        gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                 >  1:4118910/1‑93 (MQ=255)

                                                         tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccc                                >  2:1558643/1‑92 (MQ=255)

                                                         tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccc                                <  1:2608580/93‑2 (MQ=255)

                                                         tcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccc                                <  1:1565057/93‑2 (MQ=255)

                                                            tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                             >  2:1351798/1‑89 (MQ=255)

                                                               ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaa                          >  2:4146837/1‑93 (MQ=255)

                                                               ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaa                          >  2:966403/1‑93 (MQ=255)

                                                               ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaa                          >  1:2283413/1‑93 (MQ=255)

                                                                 ggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaaaa                        >  2:2430244/1‑93 (MQ=255)

                                                                 ggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaaaa                        >  1:4218384/1‑93 (MQ=255)

                                                                 ggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaaaa                        <  1:5533450/93‑1 (MQ=255)

                                                                  gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaaaaa                       >  2:4827911/1‑93 (MQ=255)

                                                                  gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaaaaa                       <  1:3624164/93‑1 (MQ=255)

                                                                  gTCGGAACGATGGTCGAAGCTAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATaaaaa                       <  1:296111/93‑1 (MQ=255)

                                                                   tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAAt                      <  1:4341061/93‑1 (MQ=255)

                                                                   tCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAAt                      >  2:2615448/1‑93 (MQ=255)

                                                                     tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGt                    <  2:678197/93‑1 (MQ=255)

                                                                     tGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATCCTCCGTACCC‑TTATAAAAATGt                    <  1:2049591/93‑1 (MQ=255)

                                                                      gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGtt                   >  1:3773178/1‑93 (MQ=255)

                                                                        aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTgc                 >  1:5892590/1‑93 (MQ=255)

                                                                        aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTgc                 <  1:4967677/93‑1 (MQ=255)

                                                                          gATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTgcgc               <  2:472333/93‑1 (MQ=255)

                                                                            tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCaa             <  2:189645/93‑1 (MQ=255)

                                                                               tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTACTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGt          >  2:5946744/1‑93 (MQ=255)

                                                                                cGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGtt         <  1:228936/93‑1 (MQ=255)

                                                                                  aaGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTaa       <  2:217755/93‑1 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACt     >  2:2490678/1‑93 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACt     >  2:2637487/1‑93 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACt     >  1:4307962/1‑93 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTTATAAAAATGTTGCGCAATGTTAACt     <  1:1492865/93‑1 (MQ=255)

                                                                                     cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACta    <  2:3060995/93‑1 (MQ=255)

                                                                                     cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACta    <  2:105036/93‑1 (MQ=255)

                                                                                     cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACta    >  2:865039/1‑93 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtat   >  1:1844938/1‑93 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtat   <  2:5011050/93‑1 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtat   <  2:109252/93‑1 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtat   <  1:615067/93‑1 (MQ=255)

                                                                                       agagGCGATGCAGCAGGTGCAAGTCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtata  <  2:5491740/93‑1 (MQ=255)

                                                                                       agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtata  >  1:200849/1‑93 (MQ=255)

                                                                                       agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtata  >  2:6132155/1‑93 (MQ=255)

                                                                                       agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtata  <  1:3346419/93‑1 (MQ=255)

                                                                                       agagGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACtata  <  1:3791954/93‑1 (MQ=255)

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013624‑4013809

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC                                                                                                 >  SRR4302532.3658007/1‑94 (MQ=60)

   AGCAGGCTTTTTATCCCCTTGGGAAGGGATAGAGGTAAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgat                                                                                              <  SRR4302532.1182671/94‑4 (MQ=60)

   AGCAGGCGTTTTATCCCTTTGTGAGGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgat                                                                                              <  SRR4302532.1935977/94‑4 (MQ=60)

     gcggtattttacCCCCTTGTGAGGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                            <  SRR4302532.5591208/82‑1 (MQ=60)

     CAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                            >  SRR4302532.5077519/1‑94 (MQ=60)

      AGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                           >  SRR4302532.4789858/1‑94 (MQ=60)

      AGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                           >  SRR4302532.1723376/1‑94 (MQ=60)

       GGCATGTTACCCCCTTGTGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                          <  SRR4302532.2541286/94‑1 (MQ=60)

        gatttttaccccctgggaagggaATAGAGTTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGC                                                                                         <  SRR4302532.1406204/71‑1 (MQ=60)

         cttgtttccccttggtgaggggataggggagaacCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                        <  SRR4302532.719331/60‑1 (MQ=60)

         ctgtttaccccttgGTGAAGGAATGGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                        <  SRR4302532.422971/80‑1 (MQ=60)

         GATGTTACCCCGTTGTGAGGGGATAGAGGTAAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                        <  SRR4302532.5122959/94‑1 (MQ=60)

          ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                       >  SRR4302532.1427943/1‑94 (MQ=60)

          ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                       >  SRR4302532.6098378/1‑94 (MQ=60)

          ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                       >  SRR4302532.5949655/1‑94 (MQ=60)

           TGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                      <  SRR4302532.4472482/94‑1 (MQ=60)

           TGTTACCCCCTGGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                      <  SRR4302532.5382261/94‑1 (MQ=60)

            tttccCCCCTTGTGAAGGGATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                     <  SRR4302532.5082705/89‑1 (MQ=60)

            tttacCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                     <  SRR4302532.5082704/89‑1 (MQ=60)

            GTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGG                                                                                     >  SRR4302532.4843316/1‑94 (MQ=60)

            GTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGG                                                                                     >  SRR4302532.2294398/1‑94 (MQ=60)

             tacccccgtggggagGGGATAGAGGTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGATAGGCGATGCAGCAGGTG                                                                                    <  SRR4302532.3410/79‑1 (MQ=60)

              TATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGC                                                                                   >  SRR4302532.4333396/1‑94 (MQ=60)

              TATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGC                                                                                   >  SRR4302532.5820579/1‑94 (MQ=60)

               ATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCA                                                                                  >  SRR4302532.4552946/1‑94 (MQ=60)

                TCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAA                                                                                 >  SRR4302532.5188967/1‑94 (MQ=60)

                  CCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                               >  SRR4302532.5739164/1‑94 (MQ=60)

                  CCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                               >  SRR4302532.974034/1‑94 (MQ=60)

                    TTTGTAAAGGGAAAGGGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCC                                                                             <  SRR4302532.3756497/94‑1 (MQ=60)

                      TGTGAAGGGAGAGGTGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                           <  SRR4302532.6259588/94‑1 (MQ=60)

                      TGTGAAGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                           <  SRR4302532.6259589/94‑1 (MQ=60)

                       agGAAGGAATAGAGTTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGA                                                                          <  SRR4302532.1875075/92‑1 (MQ=60)

                       GTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGA                                                                          >  SRR4302532.5766090/1‑94 (MQ=60)

                         gagggaatggagttgagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                        <  SRR4302532.4154049/76‑1 (MQ=60)

                         GAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                        >  SRR4302532.1653025/1‑94 (MQ=60)

                         GAAGGGAGAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                        <  SRR4302532.5397457/94‑1 (MQ=60)

                          AAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                       >  SRR4302532.2475262/1‑94 (MQ=60)

                          AAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                       >  SRR4302532.1612645/1‑94 (MQ=60)

                          AAGGAATAGAGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                       <  SRR4302532.3927374/94‑1 (MQ=60)

                            GGGATAGAGTTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                     <  SRR4302532.4487419/94‑1 (MQ=60)

                            GGGAAAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                     <  SRR4302532.4799447/94‑1 (MQ=60)

                            GGAATAGAGGGAAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                     <  SRR4302532.5640343/94‑1 (MQ=60)

                              GATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                   <  SRR4302532.919917/94‑1 (MQ=60)

                               ATAGAGGTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                  <  SRR4302532.3994601/94‑1 (MQ=60)

                               ATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTG                                                                  >  SRR4302532.2324195/1‑94 (MQ=60)

                               ATAGAAATGAAGCCCCCCACAATCGCCTAGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCgatgacgcagacgcaaatccagcatgatgtg                                                                  >  SRR4302532.4426323/1‑63 (MQ=52)

                                 AGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                                >  SRR4302532.150722/1‑94 (MQ=60)

                                 AGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                                >  SRR4302532.1376130/1‑94 (MQ=60)

                                  GAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTG                                                               >  SRR4302532.2319793/1‑94 (MQ=60)

                                   GGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                              <  SRR4302532.182158/94‑1 (MQ=60)

                                    GGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                             <  SRR4302532.5049101/94‑1 (MQ=60)

                                    GGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                             <  SRR4302532.3593025/94‑1 (MQ=60)

                                     gtaaccCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                            <  SRR4302532.1698196/88‑1 (MQ=60)

                                     GTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                            <  SRR4302532.302240/94‑1 (MQ=60)

                                      TGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGC                                                           >  SRR4302532.2960799/1‑94 (MQ=60)

                                       GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                          >  SRR4302532.982710/1‑94 (MQ=60)

                                        AGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                         <  SRR4302532.5062808/94‑1 (MQ=60)

                                        AAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCA                                                         >  SRR4302532.5574894/1‑94 (MQ=60)

                                         AGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                        >  SRR4302532.5896260/1‑94 (MQ=60)

                                         AGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                        >  SRR4302532.3273391/1‑94 (MQ=60)

                                          GCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATG                                                       >  SRR4302532.5903744/1‑94 (MQ=60)

                                           CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                      <  SRR4302532.1282615/94‑1 (MQ=60)

                                           CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                      <  SRR4302532.4662344/94‑1 (MQ=60)

                                           CCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGG                                                      <  SRR4302532.2971941/94‑1 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                     >  SRR4302532.976478/1‑94 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                     >  SRR4302532.2104002/1‑94 (MQ=60)

                                            CCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGT                                                     <  SRR4302532.4884577/94‑1 (MQ=60)

                                             CGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTA                                                    >  SRR4302532.3502405/1‑94 (MQ=60)

                                             CGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTA                                                    <  SRR4302532.2297999/94‑1 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                   >  SRR4302532.5242562/1‑94 (MQ=60)

                                              GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                   <  SRR4302532.4727329/94‑1 (MQ=60)

                                               CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                  >  SRR4302532.2831970/1‑94 (MQ=60)

                                                CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                 <  SRR4302532.4120709/94‑1 (MQ=60)

                                                CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                 >  SRR4302532.1454910/1‑94 (MQ=60)

                                                CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                 >  SRR4302532.5264801/1‑94 (MQ=60)

                                                  CAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTC                                               >  SRR4302532.3561688/1‑94 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                              <  SRR4302532.5602076/94‑1 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                              <  SRR4302532.1927379/94‑1 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                              <  SRR4302532.5548793/94‑1 (MQ=60)

                                                   AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                              <  SRR4302532.4505187/94‑1 (MQ=60)

                                                    ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG                                             <  SRR4302532.3701760/94‑1 (MQ=60)

                                                        CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                        >  SRR4302532.4257143/1‑94 (MQ=60)

                                                        CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                        >  SRR4302532.1062426/1‑94 (MQ=60)

                                                          TCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑CT                                     <  SRR4302532.2695824/94‑1 (MQ=60)

                                                          TCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑CT                                     >  SRR4302532.3646071/1‑94 (MQ=60)

                                                          TCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑CT                                     <  SRR4302532.1618005/94‑1 (MQ=60)

                                                           CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCctt                                     <  SRR4302532.1972166/94‑4 (MQ=60)

                                                           CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCctt                                     >  SRR4302532.245117/1‑91 (MQ=60)

                                                             TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTAT                                   <  SRR4302532.284790/94‑1 (MQ=60)

                                                             TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTAT                                   <  SRR4302532.6281521/94‑1 (MQ=60)

                                                             TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTAT                                   >  SRR4302532.3227105/1‑94 (MQ=60)

                                                             GCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTAT                                   <  SRR4302532.626717/94‑1 (MQ=60)

                                                              CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATA                                  <  SRR4302532.5162731/94‑1 (MQ=60)

                                                              CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATA                                  <  SRR4302532.894160/94‑1 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAA                                 >  SRR4302532.2618937/1‑94 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAA                                 >  SRR4302532.2359196/1‑94 (MQ=60)

                                                               CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAA                                 >  SRR4302532.3851637/1‑94 (MQ=60)

                                                                 GGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAA                               >  SRR4302532.4359971/1‑94 (MQ=60)

                                                                  GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAA                              <  SRR4302532.5725475/94‑1 (MQ=60)

                                                                  GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAA                              >  SRR4302532.4596472/1‑94 (MQ=60)

                                                                   GTCTGAAGGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAAT                             <  SRR4302532.3745602/94‑1 (MQ=60)

                                                                   GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAAT                             <  SRR4302532.3745599/94‑1 (MQ=60)

                                                                   GTCGGAACGATGGTCGAAGCTAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAAT                             <  SRR4302532.306323/94‑1 (MQ=60)

                                                                    TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATG                            <  SRR4302532.4487686/94‑1 (MQ=60)

                                                                     CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGT                           <  SRR4302532.697755/94‑1 (MQ=60)

                                                                     CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGT                           <  SRR4302532.3864065/94‑1 (MQ=60)

                                                                      TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTT                          >  SRR4302532.3900215/1‑94 (MQ=60)

                                                                      TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATCCTCCGTACCC‑TTATAAAAATGTT                          <  SRR4302532.2117845/94‑1 (MQ=60)

                                                                        AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGC                        >  SRR4302532.6096658/1‑94 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCG                       <  SRR4302532.5138005/94‑1 (MQ=60)

                                                                         ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCG                       <  SRR4302532.4859788/94‑1 (MQ=60)

                                                                           GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCA                     <  SRR4302532.2055750/94‑1 (MQ=60)

                                                                             TGGTCGAAGCGAGAGGCGATGCATCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAAT                   <  SRR4302532.4448233/94‑1 (MQ=60)

                                                                              GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATG                  <  SRR4302532.5794588/94‑1 (MQ=60)

                                                                              GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATG                  <  SRR4302532.4709930/94‑1 (MQ=60)

                                                                               GTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGT                 <  SRR4302532.3045970/94‑1 (MQ=60)

                                                                                 CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTA               <  SRR4302532.236748/94‑1 (MQ=60)

                                                                                 CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTA               <  SRR4302532.236749/94‑1 (MQ=60)

                                                                                   AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAAC             <  SRR4302532.11844/94‑1 (MQ=60)

                                                                                   AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAAC             >  SRR4302532.2988981/1‑94 (MQ=60)

                                                                                   AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAAC             <  SRR4302532.5964373/94‑1 (MQ=60)

                                                                                    AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACT            >  SRR4302532.4453611/1‑94 (MQ=60)

                                                                                     GCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTTATAAAAATGTTGCGCAATGTTAACTA           <  SRR4302532.1543228/94‑1 (MQ=60)

                                                                                      CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT          >  SRR4302532.2162015/1‑94 (MQ=60)

                                                                                      CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302532.1391841/94‑1 (MQ=60)

                                                                                      CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302532.2795273/94‑1 (MQ=60)

                                                                                      CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTAT          >  SRR4302532.1906197/1‑94 (MQ=60)

                                                                                       GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA         <  SRR4302532.636509/94‑1 (MQ=60)

                                                                                       GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302532.207706/1‑94 (MQ=60)

                                                                                       GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302532.3034030/1‑94 (MQ=60)

                                                                                       GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302532.3900455/1‑94 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302532.3458129/94‑1 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302532.2307035/94‑1 (MQ=60)

                                                                                        AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAG        <  SRR4302532.3919898/94‑1 (MQ=60)

                                                                                         GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGT       >  SRR4302532.1538688/1‑94 (MQ=60)

                                                                                         GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGT       <  SRR4302532.4526715/94‑1 (MQ=60)

                                                                                             G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302532.5269776/1‑94 (MQ=60)

GTAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC‑TTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013624‑4013809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`