Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	593	264.2	13311096	97.8%	13018251	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,013,717	+G	coding (3/999 nt)	ysgA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,013,717	1	.	G	100.0%	821.0 / NA	224	M1M (ATG→ATC) †	ysgA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (114/110); total (114/110)

Only 100 of 239 total aligned reads displayed.

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

 aaGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcg                                                                                        >  1:3875825/1‑92 (MQ=255)

  aGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcga                                                                                       >  2:1884549/1‑91 (MQ=255)

  aGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcga                                                                                       >  1:2759615/1‑91 (MQ=255)

  aGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcga                                                                                       >  1:1997175/1‑91 (MQ=255)

    cAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGTCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcgatg                                                                                     >  2:3569649/1‑89 (MQ=255)

    cAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGcgatg                                                                                     >  1:2495126/1‑89 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                   >  2:4926321/1‑93 (MQ=255)

      ggCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTAGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                   >  2:2897876/1‑93 (MQ=255)

      ggCATGTTACCCCTTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgca                                                                                   <  1:5760197/93‑1 (MQ=255)

       gCATGTTACCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcag                                                                                  <  1:871051/93‑1 (MQ=255)

         tttttATCCCCTTGTGAGGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATgcagca                                                                                <  1:1899824/90‑1 (MQ=255)

          tGTTATCCCTTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                               <  2:2612345/93‑1 (MQ=255)

          tGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                               >  2:503427/1‑93 (MQ=255)

          tGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                               >  2:2980281/1‑93 (MQ=255)

          tGTTATCCCCTTGGGAAGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                               <  1:5023446/93‑1 (MQ=255)

          tGTTACCCCTTGGAGAGGGGAAAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGcagcag                                                                               <  1:106341/93‑1 (MQ=255)

            ttACCCCGTTGTGAAGGGAAAGGGTTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGt                                                                             <  1:152830/93‑1 (MQ=255)

              aTCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGc                                                                           >  2:5855115/1‑93 (MQ=255)

               tCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCa                                                                          >  2:3330719/1‑93 (MQ=255)

                ccccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaa                                                                         >  2:3214221/1‑93 (MQ=255)

                 cccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCaaa                                                                        >  1:732226/1‑93 (MQ=255)

                  ccTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAt                                                                       >  2:4383924/1‑93 (MQ=255)

                    ttGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATcc                                                                     >  2:1434662/1‑93 (MQ=255)

                    ttGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGAAAATcc                                                                     >  2:1709508/1‑93 (MQ=255)

                    ttGTAAAGGGATAGAGCTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATcc                                                                     <  2:6331246/93‑1 (MQ=255)

                        gAAGGTAGAGCGGAGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                 <  1:5790875/93‑1 (MQ=255)

                        gAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAt                                                                 >  1:5723988/1‑93 (MQ=255)

                         aaGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAtt                                                                <  2:2742995/93‑1 (MQ=255)

                           gggATAGGGGTAACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgt                                                              <  1:5507359/93‑1 (MQ=255)

                           ggaaTAGGGGTAAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgt                                                              <  2:6165531/90‑1 (MQ=255)

                           ggaaTAGAGGTAAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgt                                                              <  2:5105121/90‑1 (MQ=255)

                            ggATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgtt                                                             >  2:2029083/1‑93 (MQ=255)

                            ggATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgtt                                                             >  1:6293439/1‑93 (MQ=255)

                             gATAGAGCTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAttgttg                                                            <  1:5374234/93‑1 (MQ=255)

                              aTAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgt                                                           >  1:320750/1‑93 (MQ=255)

                                 gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                        >  2:623654/1‑93 (MQ=255)

                                 gagGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgtt                                                        <  2:5383013/93‑1 (MQ=255)

                                  gggTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATtgttgtgttg                                                       <  1:3499297/92‑1 (MQ=255)

                                   ggTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTgttgtgttgt                                                      <  1:1084464/93‑1 (MQ=255)

                                   ggTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTgttgtgttgt                                                      >  2:6085560/1‑93 (MQ=255)

                                    gTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTgttgtt                                                     <  1:4845107/93‑1 (MQ=255)

                                     ggAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGttgttg                                                    <  1:6185188/92‑1 (MQ=255)

                                      gaggCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                   <  2:5540083/90‑1 (MQ=255)

                                      gAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                   <  1:3828055/93‑1 (MQ=255)

                                      gAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGc                                                   >  2:4210990/1‑93 (MQ=255)

                                       aaGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGCGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                  >  1:6264307/1‑93 (MQ=255)

                                       aaGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                  >  1:5607346/1‑93 (MQ=255)

                                       aaGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                  >  2:3825859/1‑93 (MQ=255)

                                       aaGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGcc                                                  >  1:1145546/1‑93 (MQ=255)

                                        accccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                 <  1:5990055/91‑1 (MQ=255)

                                        aGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCa                                                 <  2:1749388/93‑1 (MQ=255)

                                         gCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAt                                                >  1:2179760/1‑93 (MQ=255)

                                         gCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAt                                                <  2:6431046/93‑1 (MQ=255)

                                           ccGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATgg                                              >  2:6120866/1‑93 (MQ=255)

                                            cGCCACAATCGCGTCGTCCGGGGTCTAAACTATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGt                                             <  2:2568257/93‑1 (MQ=255)

                                              ccACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                           >  2:4191513/1‑93 (MQ=255)

                                              ccACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                           >  2:3638482/1‑93 (MQ=255)

                                              ccACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                           >  1:1838480/1‑93 (MQ=255)

                                              ccACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAt                                           <  1:4043845/93‑1 (MQ=255)

                                               cacaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAtt                                          <  1:1391144/93‑1 (MQ=255)

                                                acaATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtc                                         >  2:4808434/1‑93 (MQ=255)

                                                 caATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtct                                        <  1:2651170/93‑1 (MQ=255)

                                                 caATCGCGACGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtct                                        >  1:4308389/1‑93 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                       <  2:572819/93‑1 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                       <  1:3427545/93‑1 (MQ=255)

                                                  aaTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATtctc                                       <  2:1518628/93‑1 (MQ=255)

                                                   aTCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTcc                                      >  1:1444228/1‑93 (MQ=255)

                                                      gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTa                                   >  1:1223059/1‑93 (MQ=255)

                                                      gcgTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTa                                   <  2:6035366/93‑1 (MQ=255)

                                                        gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                >  1:3598774/1‑93 (MQ=255)

                                                        gtcgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑cc                                >  1:1713965/1‑93 (MQ=255)

                                                          cgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccct                              <  1:2578078/93‑3 (MQ=255)

                                                          cgtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccct                              >  2:3496492/1‑91 (MQ=255)

                                                           gtcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctt                             <  2:453064/93‑4 (MQ=255)

                                                            tcCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑ccctta                            >  2:2153041/1‑89 (MQ=255)

                                                              cGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTtata                           >  1:4889864/1‑93 (MQ=255)

                                                               ggggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaa                          >  2:1622161/1‑93 (MQ=255)

                                                                gggTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaa                         >  2:4253796/1‑93 (MQ=255)

                                                                  gTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATaaaaa                       >  2:1347875/1‑93 (MQ=255)

                                                                      gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGtt                   >  2:2881877/1‑93 (MQ=255)

                                                                      gAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGtt                   <  2:2713496/93‑1 (MQ=255)

                                                                       aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                  >  2:1475416/1‑93 (MQ=255)

                                                                       aaCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTg                  <  2:3972014/93‑1 (MQ=255)

                                                                        aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                 >  2:1211807/1‑93 (MQ=255)

                                                                        aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                 <  1:4222315/93‑1 (MQ=255)

                                                                        aCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTgc                 <  1:3058134/93‑1 (MQ=255)

                                                                           aTGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCa              >  1:3677087/1‑93 (MQ=255)

                                                                            tGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCaa             >  1:942328/1‑93 (MQ=255)

                                                                              gTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCGCCGTACCCTTATAAAAATGTTGCGCAATg           <  1:6048286/93‑1 (MQ=255)

                                                                               tCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGt          <  2:2932177/93‑1 (MQ=255)

                                                                                  aaGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTaa       <  2:5946445/93‑1 (MQ=255)

                                                                                  aaGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTaa       <  1:2273022/93‑1 (MQ=255)

                                                                                   aGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAc      <  2:804952/93‑1 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACt     <  2:1273658/93‑1 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACt     <  1:5434150/93‑1 (MQ=255)

                                                                                    gCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACt     >  1:2864677/1‑93 (MQ=255)

                                                                                     cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta    <  2:2363408/93‑1 (MQ=255)

                                                                                     cGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACta    <  1:3539870/93‑1 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtat   >  1:5261953/1‑93 (MQ=255)

                                                                                      gagaGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACtat   >  1:2920202/1‑93 (MQ=255)

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATA  >  NC_000913/4013625‑4013802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013625‑4013809

taaggaggcgtgtagtccctTTGTGAGGGGATAGGGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑G                                                                                              <  SRR4302547.5459824/74‑1 (MQ=60)

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑G                                                                                              >  SRR4302547.3954688/1‑94 (MQ=60)

 AAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCga                                                                                              >  SRR4302547.2814925/1‑92 (MQ=60)

 AAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCga                                                                                              >  SRR4302547.2036908/1‑92 (MQ=60)

 AAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCga                                                                                              >  SRR4302547.323427/1‑92 (MQ=60)

 AAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCga                                                                                              >  SRR4302547.2313770/1‑92 (MQ=60)

  agaagccttttttaccattagtaaagggataggggagaccCCCGCCACAATCGCGTCGTCGGGGGTCTGAGCGATGGTCGAAGCGAGAGGCgat                                                                                             <  SRR4302547.5838/54‑4 (MQ=60)

   GCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATG                                                                                            >  SRR4302547.2545539/1‑94 (MQ=60)

   GCAGGATTGTTACCCCTTTGGGAAGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATG                                                                                            <  SRR4302547.4836683/94‑1 (MQ=60)

    aaggcttttaacCCCCTGGTGAAGTGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGC                                                                                           <  SRR4302547.1719579/82‑1 (MQ=60)

     agcaattttaccccctggagaaggaagagaggtaagGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          <  SRR4302547.533629/58‑1 (MQ=60)

     TGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          >  SRR4302547.5497791/1‑94 (MQ=60)

     AGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCA                                                                                          >  SRR4302547.4314370/1‑94 (MQ=60)

      gtctgtgtatccggttgggaggagaaggaggagaccCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         <  SRR4302547.1514791/58‑1 (MQ=60)

      GGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         >  SRR4302547.2926229/1‑94 (MQ=60)

      GGCATGTTACCCCTTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         <  SRR4302547.5879314/94‑1 (MQ=60)

      GGCATGTTACCCCCTTGTGAGGGGATAGAGGTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAG                                                                                         <  SRR4302547.1966696/94‑1 (MQ=60)

       GCATGTTACCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGC                                                                                        <  SRR4302547.887755/94‑1 (MQ=60)

        CATTTTACCCCCTTGGGAGGGGATAGAGTTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                       <  SRR4302547.4776061/94‑1 (MQ=60)

        CATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                       >  SRR4302547.3886616/1‑94 (MQ=60)

        CATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                       >  SRR4302547.3112505/1‑94 (MQ=60)

        CATGTTACCCCATTGAGAAGGGAAAGAGGAGAGGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCA                                                                                       <  SRR4302547.136377/94‑1 (MQ=60)

         tttTTATCCCCTTGTGAGGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                      <  SRR4302547.1937412/91‑1 (MQ=60)

         ATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAG                                                                                      >  SRR4302547.5993672/1‑94 (MQ=60)

          tttcaccccttgggaaaggaatagaggtaagcCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     <  SRR4302547.3805826/62‑1 (MQ=60)

          tgttaccccttggagaggggaaAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     <  SRR4302547.108282/72‑1 (MQ=60)

          TGTTATCCCTTTGTGAAGGGATAGAGTTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     <  SRR4302547.895099/94‑1 (MQ=60)

          TGTTATCCCCTTGGGAAGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGG                                                                                     <  SRR4302547.5127242/94‑1 (MQ=60)

           TTTATCCCCTTGGGAGGGAATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGT                                                                                    <  SRR4302547.664966/94‑1 (MQ=60)

            TTACCCCGTTGTGAAGGGAAAGGGTTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTG                                                                                   <  SRR4302547.155618/94‑1 (MQ=60)

                CCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAA                                                                               >  SRR4302547.745901/1‑94 (MQ=60)

                CCCCTTGTGAAGGGATAGAGGGGAAGCCCGCCACAATCGCGGCGTCCGGGGCCTGAACGATGGTCGAAGCGAGAGGCgaggcagcaggggcgga                                                                               >  SRR4302547.3520279/1‑77 (MQ=60)

                 cctttgaaaagggaaaggggcgacGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              <  SRR4302547.789861/70‑1 (MQ=60)

                 CCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              >  SRR4302547.6154996/1‑94 (MQ=60)

                 CCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAAT                                                                              >  SRR4302547.2808920/1‑94 (MQ=60)

                  CTTTGTGAAGGAATAGAGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATC                                                                             <  SRR4302547.3277386/94‑1 (MQ=60)

                  CCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATC                                                                             >  SRR4302547.2476569/1‑94 (MQ=60)

                   CTTGTGAAGGGATAGTGGTGAAGCCCGCCGCAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCC                                                                            >  SRR4302547.494711/1‑94 (MQ=60)

                     TGAGAGGGGATAGGGGTGAGCCCCGCCACGATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAG                                                                          <  SRR4302547.6665512/94‑1 (MQ=60)

                       TGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGAT                                                                        >  SRR4302547.5842226/1‑94 (MQ=60)

                        gagggaaaaggggaGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                       <  SRR4302547.1453154/80‑1 (MQ=60)

                        gaaggtagagcggagacGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATT                                                                       <  SRR4302547.5910659/77‑1 (MQ=60)

                         AAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                      >  SRR4302547.1467073/1‑94 (MQ=60)

                         AAGGAATTGAGTTGAACCCCGCAACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTG                                                                      <  SRR4302547.833264/94‑1 (MQ=60)

                           GGGATAGGGGTAACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                    <  SRR4302547.5621070/94‑1 (MQ=60)

                           GGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTT                                                                    >  SRR4302547.6424264/1‑94 (MQ=60)

                             GATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                  >  SRR4302547.326712/1‑94 (MQ=60)

                             GATAGAGCTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGT                                                                  <  SRR4302547.5485148/94‑1 (MQ=60)

                               TAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGT                                                                >  SRR4302547.2648114/1‑94 (MQ=60)

                                agcgggagAGCCCGCCACAATCGCGTCGGCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                               <  SRR4302547.4351292/86‑1 (MQ=60)

                                AGAGGTGAAGCCCGCAACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTT                                                               >  SRR4302547.4136739/1‑94 (MQ=60)

                                  GGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                             <  SRR4302547.3570710/94‑1 (MQ=60)

                                  AGGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGT                                                             <  SRR4302547.2181796/94‑1 (MQ=60)

                                   GGTGAGCCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302547.1106130/94‑1 (MQ=60)

                                   GGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            >  SRR4302547.3655923/1‑94 (MQ=60)

                                   GGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302547.3570107/94‑1 (MQ=60)

                                   GGTGAACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTT                                                            <  SRR4302547.5912902/94‑1 (MQ=60)

                                    GTGACGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302547.4945513/94‑1 (MQ=60)

                                    GTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTG                                                           <  SRR4302547.6331948/94‑1 (MQ=60)

                                     GGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGC                                                          <  SRR4302547.6313569/94‑1 (MQ=60)

                                      GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGCGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         >  SRR4302547.6394431/1‑94 (MQ=60)

                                      GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         >  SRR4302547.1168704/1‑94 (MQ=60)

                                      GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         >  SRR4302547.5723041/1‑94 (MQ=60)

                                      GAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCC                                                         <  SRR4302547.3906023/94‑1 (MQ=60)

                                        AGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                       >  SRR4302547.2223409/1‑94 (MQ=60)

                                        AGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                       >  SRR4302547.423317/1‑94 (MQ=60)

                                        ACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                       <  SRR4302547.6114330/94‑1 (MQ=60)

                                        ACCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCAT                                                       <  SRR4302547.3735527/94‑1 (MQ=60)

                                             GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                  <  SRR4302547.5900609/94‑1 (MQ=60)

                                             GCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTAT                                                  >  SRR4302547.1874815/1‑94 (MQ=60)

                                              CCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATT                                                 <  SRR4302547.4125958/94‑1 (MQ=60)

                                               CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                >  SRR4302547.1169236/1‑94 (MQ=60)

                                               CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                <  SRR4302547.1418986/94‑1 (MQ=60)

                                               CACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTC                                                >  SRR4302547.5554313/1‑94 (MQ=60)

                                                ACAATCGCGACGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCT                                               >  SRR4302547.4397020/1‑94 (MQ=60)

                                                 CAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTC                                              <  SRR4302547.2704474/94‑1 (MQ=60)

                                                  AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                             <  SRR4302547.3497363/94‑1 (MQ=60)

                                                  AATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCC                                             >  SRR4302547.1473113/1‑94 (MQ=60)

                                                   ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG                                            <  SRR4302547.6641128/94‑1 (MQ=60)

                                                   ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCG                                            >  SRR4302547.5802563/1‑94 (MQ=60)

                                                   ATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCA                                            >  SRR4302547.1101361/1‑94 (MQ=60)

                                                     CGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA                                          >  SRR4302547.1247736/1‑94 (MQ=60)

                                                      GCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑C                                        <  SRR4302547.5233767/94‑1 (MQ=60)

                                                       CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       <  SRR4302547.2368568/94‑1 (MQ=60)

                                                       CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       >  SRR4302547.1747667/1‑94 (MQ=60)

                                                       CGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CC                                       >  SRR4302547.3672158/1‑94 (MQ=60)

                                                          CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCctt                                    <  SRR4302547.2630102/94‑4 (MQ=60)

                                                          CGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCctt                                    <  SRR4302547.6278012/94‑4 (MQ=60)

                                                           GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTA                                    <  SRR4302547.5290263/94‑1 (MQ=60)

                                                           GTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTA                                    >  SRR4302547.3940507/1‑94 (MQ=60)

                                                            TCCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTAT                                   <  SRR4302547.581986/94‑1 (MQ=60)

                                                             CCGGGGTCTGAGCGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  <  SRR4302547.881553/94‑1 (MQ=60)

                                                             CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  <  SRR4302547.4421724/94‑1 (MQ=60)

                                                             CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  >  SRR4302547.4991141/1‑94 (MQ=60)

                                                             CCGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATA                                  <  SRR4302547.4018276/94‑1 (MQ=60)

                                                              CGGGGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAA                                 >  SRR4302547.6283058/1‑94 (MQ=60)

                                                                 GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAA                              <  SRR4302547.2748351/94‑1 (MQ=60)

                                                                 GGTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAA                              <  SRR4302547.5094988/94‑1 (MQ=60)

                                                                  GTCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAAT                             <  SRR4302547.5515723/94‑1 (MQ=60)

                                                                   TCTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATG                            >  SRR4302547.17586/1‑94 (MQ=60)

                                                                    CTGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGT                           <  SRR4302547.957525/94‑1 (MQ=60)

                                                                     TGAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTT                          <  SRR4302547.6407931/94‑1 (MQ=60)

                                                                      GAACGATGGTCGAAGCGAGATGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         <  SRR4302547.1487239/94‑1 (MQ=60)

                                                                      GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         <  SRR4302547.2156571/94‑1 (MQ=60)

                                                                      GAACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTG                         <  SRR4302547.3980106/94‑1 (MQ=60)

                                                                       AACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGC                        <  SRR4302547.1693545/94‑1 (MQ=60)

                                                                        ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       <  SRR4302547.4308552/94‑1 (MQ=60)

                                                                        ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       <  SRR4302547.3337933/94‑1 (MQ=60)

                                                                        ACGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCG                       <  SRR4302547.3118981/94‑1 (MQ=60)

                                                                         CGATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGC                      <  SRR4302547.1176334/94‑1 (MQ=60)

                                                                          GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCA                     >  SRR4302547.3752108/1‑94 (MQ=60)

                                                                          GATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCA                     >  SRR4302547.1488139/1‑94 (MQ=60)

                                                                           ATGGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAA                    >  SRR4302547.960590/1‑94 (MQ=60)

                                                                             GGTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATG                  <  SRR4302547.1910774/94‑1 (MQ=60)

                                                                              GTCGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCGCCGTACCCTTATAAAAATGTTGCGCAATGT                 <  SRR4302547.6173771/94‑1 (MQ=60)

                                                                                CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               >  SRR4302547.3804677/1‑94 (MQ=60)

                                                                                CGAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTA               >  SRR4302547.2557186/1‑94 (MQ=60)

                                                                                 GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              >  SRR4302547.3599790/1‑94 (MQ=60)

                                                                                 GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              >  SRR4302547.2959338/1‑94 (MQ=60)

                                                                                 GAAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAA              >  SRR4302547.2914927/1‑94 (MQ=60)

                                                                                  AAGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAAC             <  SRR4302547.2318992/94‑1 (MQ=60)

                                                                                   AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACT            <  SRR4302547.1113733/94‑1 (MQ=60)

                                                                                   AGCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACT            >  SRR4302547.2921896/1‑94 (MQ=60)

                                                                                    GCGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTA           <  SRR4302547.5546480/94‑1 (MQ=60)

                                                                                     CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          >  SRR4302547.1853565/1‑94 (MQ=60)

                                                                                     CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          >  SRR4302547.5370311/1‑94 (MQ=60)

                                                                                     CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          <  SRR4302547.3612131/94‑1 (MQ=60)

                                                                                     CGAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTAT          >  SRR4302547.2978430/1‑94 (MQ=60)

                                                                                      GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302547.5844648/1‑94 (MQ=60)

                                                                                      GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302547.361326/1‑94 (MQ=60)

                                                                                      GAGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATA         >  SRR4302547.5720384/1‑94 (MQ=60)

                                                                                       AGAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATCG        >  SRR4302547.6001175/1‑94 (MQ=60)

                                                                                        GAGGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGT       <  SRR4302547.3396181/94‑1 (MQ=60)

                                                                                          GGCGATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCA     <  SRR4302547.3291558/94‑1 (MQ=60)

                                                                                            G‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTACCCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  SRR4302547.1002105/1‑94 (MQ=60)

TAAGCAGGCATGTTATCCCCTTGTGAAGGGATAGAGGTGAAGCCCGCCACAATCGCGTCGTCCGGGGTCTGAACGATGGTCGAAGCGAGAGGC‑ATGCAGCAGGTGCAAATCCAGATTGTTGTGTTGTTGCCATGGTATTCTCCGTA‑CCTTATAAAAATGTTGCGCAATGTTAACTATAGTCAGCA  >  NC_000913/4013625‑4013809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`