Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	521	238.2	12299412	96.0%	11807435	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,984	+C	coding (1250/1299 nt)	trkH →	TrkH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,983	1	.	C	99.5%	686.3 / ‑3.8	194	coding (1249/1299 nt)	trkH	TrkH
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (102/91); total (103/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

Only 100 of 216 total aligned reads displayed.

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTT  >  NC_000913/4031891‑4032073

aTCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCg                                                                                             <  1:2987298/93‑1 (MQ=255)

aTCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCg                                                                                             <  2:1031521/93‑1 (MQ=255)

  cgcgCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cc                                                                                          <  1:1142694/93‑2 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                         <  1:1008900/93‑3 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                         <  2:4349889/93‑3 (MQ=255)

   gcgcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑cca                                                                                         >  1:1846895/1‑91 (MQ=255)

     gcACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑ccacg                                                                                       >  2:4893103/1‑89 (MQ=255)

       aCTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACggg                                                                                      >  2:2471840/1‑93 (MQ=255)

         tGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCg                                                                                    <  2:5909379/93‑1 (MQ=255)

          gCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGt                                                                                   <  2:2124662/93‑1 (MQ=255)

          gCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGATGGCGATTATCGCCACGGGCGt                                                                                   >  1:2252364/1‑93 (MQ=255)

           ccGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTg                                                                                  <  2:5417809/93‑1 (MQ=255)

             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGa                                                                                >  2:5800223/1‑93 (MQ=255)

             ggAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGa                                                                                >  2:2538973/1‑93 (MQ=255)

               aaCGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATg                                                                              >  2:1474159/1‑93 (MQ=255)

                 cGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGAc                                                                            <  2:2047340/93‑1 (MQ=255)

                  gTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACt                                                                           >  1:2626616/1‑93 (MQ=255)

                  gTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACt                                                                           >  1:2380470/1‑93 (MQ=255)

                   tATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACtt                                                                          >  1:2889370/1‑93 (MQ=255)

                    ttCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACttt                                                                         <  1:4303074/92‑1 (MQ=255)

                    aTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACttt                                                                         >  2:2664472/1‑93 (MQ=255)

                     tCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGCGGATTACtttt                                                                        >  1:5394846/1‑93 (MQ=255)

                      ccTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTc                                                                       <  2:2986645/93‑1 (MQ=255)

                        tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTg                                                                     >  1:5421767/1‑93 (MQ=255)

                        tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTg                                                                     >  2:1616166/1‑93 (MQ=255)

                        tCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTg                                                                     >  2:4956982/1‑93 (MQ=255)

                          gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGcc                                                                   >  1:2873653/1‑93 (MQ=255)

                          gAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGcc                                                                   >  1:435465/1‑93 (MQ=255)

                            agacGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCTTGGATGACTTTTCTGCAtt                                                                 <  1:1937209/90‑1 (MQ=255)

                            aGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCtt                                                                 >  2:5544425/1‑93 (MQ=255)

                             gCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCttt                                                                <  2:1363846/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                             >  2:4563578/1‑93 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                             <  1:5050093/93‑1 (MQ=255)

                                gTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCg                                                             >  2:3891282/1‑93 (MQ=255)

                                 gttGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGt                                                            <  1:2290024/92‑1 (MQ=255)

                                    ggtgATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                         <  1:2124879/90‑1 (MQ=255)

                                    ggggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                         <  2:4014346/93‑1 (MQ=255)

                                    ggggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                         >  2:4101909/1‑93 (MQ=255)

                                    ggggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                         >  2:80770/1‑93 (MQ=255)

                                    ggggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCgg                                                         >  1:2317608/1‑93 (MQ=255)

                                     gggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgt                                                        <  1:2968943/93‑1 (MQ=255)

                                      ggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtt                                                       <  2:3597209/93‑1 (MQ=255)

                                      ggATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGgtt                                                       <  1:3591400/93‑1 (MQ=255)

                                          ttttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGttgttg                                                   >  1:3936813/1‑93 (MQ=255)

                                           tttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGc                                                  <  2:5148316/93‑1 (MQ=255)

                                           tttCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGc                                                  <  2:3918546/93‑1 (MQ=255)

                                             tctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGa                                                >  2:2808058/1‑93 (MQ=255)

                                             tctcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGa                                                >  2:1940126/1‑93 (MQ=255)

                                               tcCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACa                                              <  1:5836391/93‑1 (MQ=255)

                                                 cGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAtt                                            <  1:2219772/93‑1 (MQ=255)

                                                  gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                           >  2:5240435/1‑93 (MQ=255)

                                                  gCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTg                                           >  1:4942762/1‑93 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                        >  1:5733955/1‑93 (MQ=255)

                                                     tATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAt                                        >  2:1083754/1‑93 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                       <  1:5569956/93‑1 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                       >  2:6103208/1‑93 (MQ=255)

                                                      aTGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATa                                       <  1:403225/93‑1 (MQ=255)

                                                       tGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATaa                                      <  2:3678724/93‑1 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                     >  1:968090/1‑93 (MQ=255)

                                                        gCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAc                                     <  1:3098828/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                   <  2:3758770/93‑1 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                   >  1:4758818/1‑93 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                   >  1:3532164/1‑93 (MQ=255)

                                                          aTTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCt                                   >  1:3291036/1‑93 (MQ=255)

                                                           ttGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTg                                  <  1:3264423/93‑1 (MQ=255)

                                                            tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                                 <  2:3573952/93‑1 (MQ=255)

                                                            tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                                 >  1:1224549/1‑93 (MQ=255)

                                                            tGGTGTTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTgg                                 <  2:1107115/93‑1 (MQ=255)

                                                               tgtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGc                              >  2:255443/1‑93 (MQ=255)

                                                                gtTTATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGcc                             >  2:1220977/1‑93 (MQ=255)

                                                                 tttATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGGCa                            >  1:1400918/1‑93 (MQ=255)

                                                                 tttATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCa                            <  2:1750788/93‑1 (MQ=255)

                                                                  ttATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAg                           >  1:4201551/1‑93 (MQ=255)

                                                                   tATTGTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAgg                          >  1:4098329/1‑93 (MQ=255)

                                                                       gTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                      <  2:4058283/93‑1 (MQ=255)

                                                                       gTCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCtt                      <  1:1745875/93‑1 (MQ=255)

                                                                        tCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTg                     <  1:848476/93‑1 (MQ=255)

                                                                        tCAGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTg                     <  2:2509142/93‑1 (MQ=255)

                                                                          aGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGc                   <  2:3297364/93‑1 (MQ=255)

                                                                          aGTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGc                   <  2:3096484/93‑1 (MQ=255)

                                                                           gTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCg                  >  1:5871779/1‑93 (MQ=255)

                                                                           gTATGCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCg                  >  1:128844/1‑93 (MQ=255)

                                                                               gCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGt              >  1:2010595/1‑93 (MQ=255)

                                                                               gCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGt              <  1:4578645/93‑1 (MQ=255)

                                                                               gCTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGt              <  1:5216730/93‑1 (MQ=255)

                                                                                cTGGCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTgggt             >  1:1632596/1‑91 (MQ=255)

                                                                                  ggCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGc           <  1:6083899/93‑1 (MQ=255)

                                                                                   gCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt          >  1:2041989/1‑93 (MQ=255)

                                                                                   gCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt          >  1:801510/1‑93 (MQ=255)

                                                                                   gCGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCt          >  2:5073278/1‑93 (MQ=255)

                                                                                    cGATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTg         <  1:722119/93‑1 (MQ=255)

                                                                                     gATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGa        >  1:1861837/1‑93 (MQ=255)

                                                                                     gATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGa        <  2:5687347/93‑1 (MQ=255)

                                                                                     gATTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGa        >  2:4709014/1‑93 (MQ=255)

                                                                                      aTTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt       >  2:3590641/1‑93 (MQ=255)

                                                                                      aTTATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAt       >  1:485340/1‑93 (MQ=255)

                                                                                       ttATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATa      >  1:1544430/1‑93 (MQ=255)

                                                                                       ttATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATa      >  1:5200015/1‑93 (MQ=255)

                                                                                        tATCGCCACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATaa     <  2:2873653/93‑1 (MQ=255)

                                                                                          tCG‑CGCGGGGGTGGATGACTTTTCTGCCTTTGCGTCGGTTGGTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACtt  <  1:4717837/93‑1 (MQ=255)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑CACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTT  >  NC_000913/4031891‑4032073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C                                                                                                >  SRR4302532.1136880/1‑94 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C                                                                                                <  SRR4302532.3086054/94‑1 (MQ=60)

  CGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Cca                                                                                              >  SRR4302532.1908211/1‑92 (MQ=60)

  CGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Cca                                                                                              <  SRR4302532.1180813/94‑3 (MQ=60)

   GCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTCTTGTCAGTATGCTTGCGATTATCG‑Ccac                                                                                             >  SRR4302532.854545/1‑91 (MQ=60)

   GCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑Ccac                                                                                             <  SRR4302532.1043212/94‑4 (MQ=60)

    CGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACG                                                                                            >  SRR4302532.3048450/1‑94 (MQ=60)

     GCACTGCCGGAACGTAGCCTCGAAGCGGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGG                                                                                           <  SRR4302532.3104008/94‑1 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGG                                                                                          >  SRR4302532.1187731/1‑94 (MQ=60)

      CACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGG                                                                                          <  SRR4302532.2630517/94‑1 (MQ=60)

         TGCCGGAGCGTATCCTCGAAGCCGTTTGGGGATTTTTGTCCCCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGG                                                                                       <  SRR4302532.5179093/94‑1 (MQ=60)

         TGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGATGGCGATTATCGCC‑ACGGGCGT                                                                                       >  SRR4302532.2327125/1‑94 (MQ=60)

          GCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTG                                                                                      <  SRR4302532.2187068/94‑1 (MQ=60)

           CCGGAACGTATCCTCGATGCCGTTTGGGGATTTTTCTCTGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGG                                                                                     <  SRR4302532.1027778/94‑1 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGG                                                                                     >  SRR4302532.411628/1‑94 (MQ=60)

           CCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGG                                                                                     >  SRR4302532.5003218/1‑94 (MQ=60)

            CGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGA                                                                                    >  SRR4302532.6067311/1‑94 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAT                                                                                   <  SRR4302532.3629228/94‑1 (MQ=60)

             GGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGAT                                                                                   <  SRR4302532.1704416/94‑1 (MQ=60)

               AACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGA                                                                                 >  SRR4302532.5972500/1‑94 (MQ=60)

                ACGTATCGTCGAAGCCGTTTGGGTATTTTTCTCCTCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCTATTATCGCC‑ACGGGCGTGGATGAC                                                                                <  SRR4302532.2306864/94‑1 (MQ=60)

                ACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGAC                                                                                >  SRR4302532.53102/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACT                                                                               >  SRR4302532.2714582/1‑94 (MQ=60)

                 CGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACT                                                                               >  SRR4302532.2459785/1‑94 (MQ=60)

                  GTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTT                                                                              >  SRR4302532.2985903/1‑94 (MQ=60)

                   TATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTT                                                                             >  SRR4302532.2847817/1‑94 (MQ=60)

                    TTCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTT                                                                            <  SRR4302532.4448545/94‑1 (MQ=60)

                    ATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGCGGATTACTTTT                                                                            >  SRR4302532.5581768/1‑94 (MQ=60)

                      CCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCT                                                                          <  SRR4302532.4750693/94‑1 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTG                                                                         >  SRR4302532.5609684/1‑94 (MQ=60)

                       CTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGGTTATTGTCAGTATGCTGGCGATTATCG‑CGACTGGCGCGGATGACTTTTCTG                                                                         >  SRR4302532.5393073/1‑94 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCGTTGGCGTTTATTGTCAGTATGCTGGCGATTGTCGCC‑ACGGGCGTGGATGACTGTTCTGCC                                                                       >  SRR4302532.1969828/1‑94 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302532.450553/1‑94 (MQ=60)

                         CGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCC                                                                       >  SRR4302532.2969745/1‑94 (MQ=60)

                           AAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTT                                                                     <  SRR4302532.4932388/94‑1 (MQ=60)

                           AAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTCGTGATTATTGTCAGTATGCTGGCGATTATCGTC‑ACGGGCGTTGATGACTTTTTTGCCCT                                                                     >  SRR4302532.4525176/1‑94 (MQ=60)

                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTT                                                                    <  SRR4302532.5077481/94‑1 (MQ=60)

                            AGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTT                                                                    >  SRR4302532.2660824/1‑94 (MQ=60)

                            AGACGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCTTGGATGACTTTTCTGCATTT                                                                    <  SRR4302532.2001662/94‑1 (MQ=60)

                                GTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGT                                                                <  SRR4302532.5223381/94‑1 (MQ=60)

                                GTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGT                                                                >  SRR4302532.2159876/1‑94 (MQ=60)

                                 TTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               <  SRR4302532.3077594/94‑1 (MQ=60)

                                 GTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTC                                                               <  SRR4302532.2366038/94‑1 (MQ=60)

                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             >  SRR4302532.2394745/1‑94 (MQ=60)

                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             >  SRR4302532.2394747/1‑94 (MQ=60)

                                   TGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGG                                                             >  SRR4302532.2394746/1‑94 (MQ=60)

                                    GGTGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGT                                                            <  SRR4302532.2195875/94‑1 (MQ=60)

                                     GGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTT                                                           <  SRR4302532.3067238/94‑1 (MQ=60)

                                      GTATTTGTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTAGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          <  SRR4302532.3711516/94‑1 (MQ=60)

                                      GGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          <  SRR4302532.3711518/94‑1 (MQ=60)

                                      GGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTG                                                          >  SRR4302532.988780/1‑94 (MQ=60)

                                       GATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGT                                                         >  SRR4302532.1492313/1‑94 (MQ=60)

                                         TTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTG                                                       >  SRR4302532.4069467/1‑94 (MQ=60)

                                          TTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGC                                                      >  SRR4302532.3348798/1‑94 (MQ=60)

                                             TCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGAC                                                   >  SRR4302532.4362531/1‑94 (MQ=60)

                                               TCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACAT                                                 <  SRR4302532.6038984/94‑1 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               >  SRR4302532.5112412/1‑94 (MQ=60)

                                                 CGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTG                                               <  SRR4302532.2293469/94‑1 (MQ=60)

                                                  gcttatggtttgttgttaATTGTCAGTATGCTGGTAATTATCGCC‑CCGGGTGTGGATTACTTTTCCGCTTTTGCGTCGGTTGTTGCGACATTGA                                              <  SRR4302532.4597693/76‑1 (MQ=25)

                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             <  SRR4302532.3240236/94‑1 (MQ=60)

                                                   CCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAA                                             <  SRR4302532.4491459/94‑1 (MQ=60)

                                                    CTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAAT                                            >  SRR4302532.5933617/1‑94 (MQ=60)

                                                     TATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATA                                           <  SRR4302532.4903511/94‑1 (MQ=60)

                                                     TATGCATTGGGGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGGGGATGGCTTTTCTGGCCTTGCGTCGGgtggttcgcttttgtcta                                           >  SRR4302532.4609020/1‑76 (MQ=46)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302532.417313/94‑1 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302532.5763370/94‑1 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302532.1266186/94‑1 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          <  SRR4302532.417312/94‑1 (MQ=60)

                                                      ATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAA                                          >  SRR4302532.1725882/1‑94 (MQ=60)

                                                       TGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAAC                                         >  SRR4302532.1001125/1‑94 (MQ=60)

                                                        GCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACC                                        <  SRR4302532.3201585/94‑1 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302532.4921657/1‑94 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302532.3650365/1‑94 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302532.3400524/1‑94 (MQ=60)

                                                         CATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCT                                       >  SRR4302532.4264173/1‑94 (MQ=60)

                                                          ATTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTG                                      >  SRR4302532.1822792/1‑94 (MQ=60)

                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302532.3374880/94‑1 (MQ=60)

                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     <  SRR4302532.3372984/94‑1 (MQ=60)

                                                           TTGGTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGG                                     >  SRR4302532.1265512/1‑94 (MQ=60)

                                                            tgattTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302532.2603337/89‑1 (MQ=60)

                                                            TAGTGGTTATTGACAGAATGCTGACGATTATCGCC‑ACGTGCGTGGATGACTTTTCTGCCGTTGCGTCGGTTGTTGCGACATTGAATAACCTGGG                                    <  SRR4302532.1360251/94‑1 (MQ=60)

                                                              GTGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGC                                  <  SRR4302532.1644919/94‑1 (MQ=60)

                                                               TGTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCC                                 <  SRR4302532.2976574/94‑1 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGGCA                                >  SRR4302532.1447855/1‑94 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                >  SRR4302532.5629793/1‑94 (MQ=60)

                                                                GTTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTAGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCA                                >  SRR4302532.6361217/1‑94 (MQ=60)

                                                                 TTTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAG                               >  SRR4302532.4342592/1‑94 (MQ=60)

                                                                  TTATTGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGG                              >  SRR4302532.4235908/1‑94 (MQ=60)

                                                                      TGTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTT                          <  SRR4302532.2329778/94‑1 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         <  SRR4302532.1915500/94‑1 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         >  SRR4302532.772929/1‑94 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         <  SRR4302532.4261378/94‑1 (MQ=60)

                                                                       GTCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTG                         <  SRR4302532.1804594/94‑1 (MQ=60)

                                                                        TCAGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGG                        <  SRR4302532.877646/94‑1 (MQ=60)

                                                                          AGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      >  SRR4302532.133197/1‑94 (MQ=60)

                                                                          AGTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCG                      >  SRR4302532.6075242/1‑94 (MQ=60)

                                                                           GTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGT                     >  SRR4302532.5588200/1‑94 (MQ=60)

                                                                           GTATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGT                     <  SRR4302532.2472814/94‑1 (MQ=60)

                                                                            TATGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTG                    <  SRR4302532.6108410/94‑1 (MQ=60)

                                                                              TGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGT                  >  SRR4302532.2077652/1‑94 (MQ=60)

                                                                              TGCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGT                  >  SRR4302532.2077651/1‑94 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302532.5585503/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302532.5458862/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302532.5396643/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302532.4734765/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTT                 <  SRR4302532.4044737/94‑1 (MQ=60)

                                                                               GCTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGGT                 >  SRR4302532.1687857/1‑94 (MQ=60)

                                                                                CTGGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTG                >  SRR4302532.5918496/1‑94 (MQ=60)

                                                                                  GGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              >  SRR4302532.828894/1‑94 (MQ=60)

                                                                                  GGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              >  SRR4302532.2109995/1‑94 (MQ=60)

                                                                                  GGCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCT              <  SRR4302532.6293982/94‑1 (MQ=60)

                                                                                   GCGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTG             >  SRR4302532.2270237/1‑94 (MQ=60)

                                                                                    CGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGA            >  SRR4302532.1923546/1‑94 (MQ=60)

                                                                                    CGATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGA            <  SRR4302532.746758/94‑1 (MQ=60)

                                                                                     GATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302532.2012999/1‑94 (MQ=60)

                                                                                     GATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302532.502284/1‑94 (MQ=60)

                                                                                     GATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGAT           >  SRR4302532.6351645/1‑94 (MQ=60)

                                                                                      ATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA          >  SRR4302532.5379317/1‑94 (MQ=60)

                                                                                      ATTATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATA          >  SRR4302532.1596614/1‑94 (MQ=60)

                                                                                         ATCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACT       <  SRR4302532.385540/94‑1 (MQ=60)

                                                                                          TCGCC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTT      <  SRR4302532.5590616/94‑1 (MQ=60)

                                                                                          TCG‑C‑GCGGGGGTGGATGACTTTTCTGCCTTTGCGTCGGTTGGTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     <  SRR4302532.4878811/94‑1 (MQ=60)

                                                                                           cgcC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     <  SRR4302532.2739205/91‑1 (MQ=60)

                                                                                           cgcC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTT     >  SRR4302532.4016093/4‑94 (MQ=60)

                                                                                            gcC‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTA    <  SRR4302532.1232070/92‑1 (MQ=60)

                                                                                            gcC‑ACGGGCGTGGATGACTATTCTGCCTTTGCGTCGGTTGTTACGACTTTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTAATACCTTTA    <  SRR4302532.5670842/92‑1 (MQ=60)

                                                                                              C‑ACGGGCGTGTATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  <  SRR4302532.4373338/94‑1 (MQ=60)

                                                                                              C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  SRR4302532.2504518/1‑94 (MQ=60)

                                                                                              C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  <  SRR4302532.5578835/94‑1 (MQ=60)

ATCGCGCACTGCCGGAACGTATCCTCGAAGCCGTTTGGGGATTTTTCTCCGCCTATGCATTGGTGTTTATTGTCAGTATGCTGGCGATTATCG‑C‑ACGGGCGTGGATGACTTTTCTGCCTTTGCGTCGGTTGTTGCGACATTGAATAACCTGGGGCCAGGGCTTGGCGTGGTTGCTGATAACTTTACC  >  NC_000913/4031891‑4032077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`